pycom08g11460

Enhancer of polycomb-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0010036
Physical Location & Seq
Reverse (-)
9674675 .. 9680213
5539 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g11460.1

Sequence Viewer

Length: 5004 bp
ATGGAAAATAGAGTAGAGAACTCACATGGCACTGAAATTCCCGAAAAATCGAGATCTTTGGATCTGAAGAGTTTGTATAAATCGAGAAGTAGGAAAGATGTCGAAAACAAGAGCTTGAAGAGAAAAGTTAGTGCCGAGGATGGTGATGAGAACGGGGGCAAGACAAAGAAGAGTAAGAAAGAGGCTTCTCTTAGTAGTTTGAAGAATGTTAATACTAGTAGCAAAAAGAGTTTAGATAAAGTGTATCATAGCGGGTTGAATTCTGGTTCACATGATCCAGAGTCATGGAAATCTGGGTCGAGTGATAGATTGGATAGCAGTAGTGGGTTGAATGGTGTTTCGTCGCTTAGTTTGAATAATAAGGTTATTCAAATCCCGAGGCGTAAACGGGGTTTTCTAGTGAGGAAGAAATTTGATGGTGGTCAGGCACCGAAGCTGCCAGATGAATCTGCTGGTAAAGTGGGTGTCGTTGATCAGACTCATCAGATAGCTAAGTTAAATGGTGATGATTTAGGTACTCAATCTGAGTCCTTGAAAGTTAAACGAAAGAAGGGTCGGCATGATTTCAAAGAAAACATAAATAATGAGTTGAATTCAGCACCACATGCTAAGAAAGAAGACGTTCCTACAAGTCATTCGGCTGTAAGCAATGGTGATTCATCTTTGAAGAAGTCTCGGAGGAATCGTAGGAAGAGGAAGGAGTTGGCACCTGACAGTAAAAGCTCTGAAAAGGAAGCTGAGCCTTTGGTTGATAGTTCTATGAAAAAAGGCCATGATTTACAAGAAGATGATGAGGAGAATCTTGAACAGAATGCAGCAAGGATGCTTTCATCAAGGTTTGATCCCAGCTGCACTGGGTTTTCTTCAAACAACAAGGCTTCTGCAAATGGGTTGTCTTTTTTGTTGTCTTCTGGACAGGATTTTGATAGCCATAGGTCAAAGTCTATTTCTGGATCAGAATCTCCTTCAGTTGACAATTCTGGTAGAGTATTGAGGCCAAGGAAACAACACGATGAAAAGGGGCATTCACGGAAAAGGCGCCATTTCTATGAAGTTTTCTTTGGGAACTTGGATGCTTACTGGGTATTGAACCGGAGAATCAAAGTCTTCTGGCCTTTAGACCAAAGTTGGTACTATGGTCTTATTAATGACTATGACAAGGAAAAAAAACTTCATCATGTGAAATACGATGATCGTGACGAAGAATGGGTTGACCTTCAAAATGAGAGGTTCAAGCTCTTGTTACTCCCTAGTGAAGTTCCCGGTAGGACAGAGCGGAAAAAATCAAAGGTGCGGAATAGAAGTCCCGATGAGAGAAAAGGAGACATGAAATGCAGAAAAGAAATGAAAAAGAGAGAATTGACTTTGGGGGATGATAGTGGCATAGGCAGCTATATAGACACTGAACCCATTATTTCATGGTTGGCACGATCTACGGGTCGGGTCAAGTCTCCTTCCCGTGTTGTGAAGAAGCAAAAAACATCTGGTCTATCTTTAAAGCCTGTGCCACTATTGTCTGATGAAGATGCCACTTTACATGAATCTTTGGGTGACTGCTCCTTTAAAAGGGATAAAAAAATTTCTCGCCATCCTGGCAGATCATCTGATGATGTGATGCTAGAAAAGCCTACCTCACAAGGCAGCACTGGCTCCAAAGATAGCAAAATGCCTATTGTTTATGTTCGAAGGCGGCTTCGGAAGAATGAGTCTGAATTGTTTCATACATCCAAGGATGACCATGACTCTGCGAGTAAACTTGGGTCACTATATGATTTCTTGGGAAGCTTGGATGCCAATGGACCATTGTGGTCTATTGATGATGTAGGACTGCTGAAATTAACTCCTCCACGGATAGAACCAGGAAGAGTTACTTTTGAGTTAGGCCTTCCAGTGCATTCAATTATTAATGATTCCTTTAGAGTAGAGTTTTGGTTGTTTCGTGCCGCAATGCTGCGTCAGTATGGCGCAGTGGTGATTTCATGGCCAAAGGTTTATTTGGAGATGCTTTTTGTTGACAATGTTGTTGGACTGAGATTTCTCCTGTTTGAAGGTTGCTTGAAGCAGGCTGTAGCCTTTGTTTTTCTTGTCCTGTCATTGTTTCATCAACCTAATGAGCAGGGGAAGTTTATTGATTTCCAGTTACCAGCAACTTCAATCAGGTTTAAATTCTCCAGTGTTCAGCATCTTGGGAAGCAACTTGTGTTTGCATTCTACAACTTTTCTGAAGTAAAGAATTCAAAGTGGAAGTACCTAGACTCTAAACTTACGAGCCATTGTTTGCTTACCAAGAAACTGCCCCCGTCTGAATGCACCTATGATAGTATTAAAGCACTTAAAAATGGAAGAAATCAGTCACCTTTTATGTCTCTTTGCGGGAATTCCTCCTTTGTCAAGGGTACACAGAGTAGGCCCAGGCAGGGTATTAACTTCAAGGGGAGTTTCAGGGAATCTATATCTGTAAATAGTAGTCATTCTACTTCTCGTGATGATGAGCTCTGCAGAAAACTTCCTCCGCTTGCCCTCTCTTTTGCTGCTGCACCTACTTTCTTCATTAGTTTGCATCTTAAGCTGCTTATGGAAAATTGTGTTGCTAATATCTGTTTTCGGGACCGTGATTCAGTGGAGCATGTCGAAAACTGTGACAATATGTTGGCAGTTGACTGGTCTGTGGTCAAGGACTTTATTAATGGAGGTTCGAAGATTACTCCTGAGAAAAATTTGAAGGCTCCACCAAGTAATGCTACTTCTGATGGATCCTGTGCCAAGCCAGATGCAGACAATGCTATTTCTCTATGCCATGGAACTCGGACAAAGTCATCTCAGCATTTCCAAAATGGCAGTCTTGATGTTTCTGTATCCTCTGATGGTACTGGTGTCCTTGAAAAGACTGGAACAGATAAAGCTGTACAGTTGAAAGCGTTGCAAAGCCATCATCCAGAGTCAGATCGATGTTCTTTATCCCCAAGGCCTTTGGTTGGTAGAGACAAGTCTGACACTGATTCCCAATCTTTTCCGAATGGTCTCACTGTCGAAATTCCATCATTTGACCGATATGAGAAGCCTGTGGATAGGGAGGTGCAAAGTGCTCAACAGCCAACAGAATTTTCTTGGAATATGAGTGGTAGCATTATTCCCAGCTCTAACCCCACTGCTCCCAGAAGTACAGGGCACCGAAATAGAAATAGTTCATCACTTGGACACCTCTCAAATAGTTGGACGGATGGAAAAGCTGACCTTTTCCATAATGGTCTTGGCAGTGGACCAAAGAAGCCCCGAACTCAGGTGTCCTATACATTGCCTTATGGAGGTTTTGACTTTAGTTCGAAGCAAAGAAACCTCCAGAAGGGGCTTTCTCATAAACGAATCAGGAGGGCTAATAATGAGAAGAGGTCATCAGATGCTTCTAGAGGTTCCCAAAGAAATTTGGAACTCTTATCATGCGAAGCAAATGTGTTAGTCAATGGCAGTGACAGAGGGTGGAGGGAGTGTGGGGCCCATGTTGTATTAGAGCTTTTTGATCATCATGAGTGGAAGCTTGCTGTAAAAATTTCAGGGACTACAAAGTACTCATACAAAGCACATCAATTTTTGCAGCCTGGGACAACAAACCGCTACACTCATGCTATGATGTGGAAAGGAGGAAAGGACTGGAATTGGGGTTTAGAGTTTCCAGATAGGAGTCAGTGGGCACTATTTAGGGAGATGCATGAAGAGTGTTACAATCGGAATATCCGCTCTGCTTCAGTAAAAAACATTCCTATTCCTGGTGTCCGCTTAATTGAAGAAAGTGATGATAATTCAATTGAAATATCATTTCTTCGCAGTTCTGCAAAGTATTTCCGGCAAATAGAAACTGATGTTGAAATGGCTTTGGATCCATCTCGGGTATTGTATGATATGGATAGTGATGATGAGCAGTGGATTTTGAAGTTTCAAAATTCTTCTGAAGTTCACAACAGTAGCTCAACAGAGATTGACGAGGAGATGTTTGAGAAAACAATGGACGTGTTTGAAAAGGCTGCATATGATCAACAGTGTGATGAGTTCACATCTGAAGAAATAGAAGAGCTCATGGCTGGTGCTGGAGTTGGGCCCATGGATGTGATTTTATCCATTTATGAGCATTGGCTACAGAAAAGGCAGAGGAAGGGAATGCCTTTAATCCGACATCTTCAGCCACCATCTTGGGAAAGGTATCAACAAGAAGTAAAAGAGTGGGAGCAAGCTATGATCGAAACCAATACCACCCTCCCCAATGGATGCTACGGGAAACCTGCATCAGTTGAGAAGCCACCAATGTTTGCTTTTTGTCTGAAACCCCGGGGACTAGAAGTTCCCAACAAGGGATCAAAACAACGGTCACAGAAGAAATTTTCGCTTTCTGGACATAATGGTGCCATGTTAGGAGATCACGATGGTTTCCATGCTATTGGAAGAAGATCAAATGGGTTTGCTTTTGGTGATGAAAGGCTGGCATATCCAGGCCATAACTATGACTCTTTAGACGACTCCCCCTTGTCTCAGACATCACCTGGGGTATTTTCACCAAGGGATGCAGCTAACATCTTGGTGAGCAATGATGGGTTTGAGAGAAATCATCTCCGCAGAATTCATAGAAGCAAGTCAAAGAAATTTGCGAGGACAGTATCTTATGTTGCCCCCCAGATGGTGTCTTCATACAGTCCTAGAGTCGTAGGAAACAGAAATGAATTTCATCGATGGAATGCAGATATCCCTGATTGGTCAAGCCAGCGTTATTATCAACCAGAAGTGTCTCCGAGGCATGGCATGGGACTGTTGGATGATTCTGATCTCGATGAGTTCAGACTGCGTGATGCATCTGGTGCCGCTCAACATGCACATAAAATGGCAAGGCTCAAGAGAGAGAGAGCTCAGAGATTGTTTTACAGAGCAGATCTAGCAATACACAGGGCCGTGGTTTCTCTCATGACCGCGGAAGCGATCAAAACTTCTTCTGAGGACTCCAGTGACGATGAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPL1

Protein Analysis

1668

Amino Acids

188.2

Weight (kDa)

9.09

Isoelectric Point (pI)

50.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPL1 PF10513 1243 - 1339 8.2e-12 Enhancer of polycomb-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 4282
AccB1I GGYRCC 6 cut(s) 427, 706, 1038, 3168, 4394, 4844
AccBSI CCGCTC 3 cut(s) 1276, 3735, 4850
AccII CGCG 1 cut(s) 4955
AclWI GGATC 9 cut(s) 69, 269, 836, 961, 2748, 2761, 3869, 3882, 4354
AcoI YGGCCR 1 cut(s) 1982
AcuI CTGAAG 7 cut(s) 86, 951, 2244, 3726, 3966, 4074, 4157
AcyI GRCGYC 1 cut(s) 1039
AfaI GTAC 8 cut(s) 517, 1133, 2249, 2398, 2869, 2907, 3163, 3566
AflII CTTAAG 1 cut(s) 2564
AflIII ACRYGT 1 cut(s) 4005
AhlI ACTAGT 1 cut(s) 215
AjiI CACGTC 1 cut(s) 4006
AjnI CCWGG 7 cut(s) 1591, 1858, 2411, 3595, 3763, 4480, 4531
AjuI GAANNNNNNNTTGG 4 cut(s) 1044, 1076, 3088, 3120
AloI GAACNNNNNNTCC 2 cut(s) 4317, 4349
Alw21I GWGCWC 4 cut(s) 2496, 3088, 4071, 4894
Alw26I GTCTC 8 cut(s) 678, 1317, 1455, 2370, 2976, 3026, 4524, 4779
AlwI GGATC 9 cut(s) 69, 269, 836, 961, 2748, 2761, 3869, 3882, 4354
Ama87I CYCGRG 3 cut(s) 376, 3882, 4320
ApaI GGGCCC 2 cut(s) 3496, 4095
ArsI GACNNNNNNTTYG 8 cut(s) 538, 570, 878, 910, 2824, 2856, 4343, 4375
AseI ATTAAT 3 cut(s) 1146, 1905, 2685
Asp700I GAANNNNTTC 3 cut(s) 621, 1716, 3184
AspLEI GCGC 2 cut(s) 1041, 1967
AspS9I GGNCC 9 cut(s) 1799, 2409, 2608, 3260, 3492, 3493, 4091, 4092, 4932
AsuC2I CCSGG 3 cut(s) 1263, 4321, 4322
AsuII TTCGAA 3 cut(s) 1684, 2696, 3323
AvaI CYCGRG 3 cut(s) 376, 3882, 4320
AvaII GGWCC 3 cut(s) 1799, 2608, 3260
BaeGI GKGCMC 4 cut(s) 3171, 3496, 3691, 4095
BaeI ACNNNNGTAYC 4 cut(s) 1123, 1156, 2859, 2892
BalI TGGCCA 1 cut(s) 1984
BamHI GGATCC 2 cut(s) 2753, 3874
BanI GGYRCC 6 cut(s) 427, 706, 1038, 3168, 4394, 4844
BanII GRGCYC 5 cut(s) 2496, 3496, 4071, 4095, 4894
BarI GAAGNNNNNNTAC 2 cut(s) 2728, 2760
BauI CACGAG 1 cut(s) 2481
BbsI GAAGAC 4 cut(s) 624, 900, 1099, 4665
Bbv12I GWGCWC 4 cut(s) 2496, 3088, 4071, 4894
BceAI ACGGC 1 cut(s) 4919
BciT130I CCWGG 7 cut(s) 1593, 1860, 2413, 3597, 3765, 4482, 4533
BciVI GTATCC 1 cut(s) 2866
BclI TGATCA 3 cut(s) 472, 3517, 4027
BcnI CCSGG 3 cut(s) 1263, 4321, 4322
BcoDI GTCTC 8 cut(s) 678, 1317, 1455, 2370, 2976, 3026, 4524, 4779
BcuI ACTAGT 1 cut(s) 215
BfaI CTAG 9 cut(s) 216, 398, 1251, 1619, 2252, 3405, 4328, 4686, 4919
BfmI CTRYAG 3 cut(s) 2067, 2497, 4130
BfoI RGCGCY 1 cut(s) 1042
BfrI CTTAAG 1 cut(s) 2564
BfuAI ACCTGC 1 cut(s) 4282
BfuI GTATCC 1 cut(s) 2866
BglI GCCNNNNNGGC 1 cut(s) 1593
BglII AGATCT 2 cut(s) 53, 4915
BlpI GCTNAGC 1 cut(s) 738
BmcAI AGTACT 1 cut(s) 3566
Bme18I GGWCC 3 cut(s) 1799, 2608, 3260
BmeT110I CYCGRG 3 cut(s) 376, 3882, 4320
BmgBI CACGTC 1 cut(s) 4006
BmgT120I GGNCC 9 cut(s) 1799, 2409, 2608, 3260, 3492, 3493, 4091, 4092, 4932
BmrI ACTGGG 2 cut(s) 864, 1090
BmuI ACTGGG 2 cut(s) 864, 1090
BpiI GAAGAC 4 cut(s) 624, 900, 1099, 4665
BpmI CTGGAG 4 cut(s) 2155, 3323, 4104, 4969
Bpu1102I GCTNAGC 1 cut(s) 738
Bpu14I TTCGAA 3 cut(s) 1684, 2696, 3323
BpuEI CTTGAG 1 cut(s) 4862
BpuMI CCSGG 3 cut(s) 1263, 4321, 4322
Bsa29I ATCGAT 2 cut(s) 2947, 4717
BsaBI GATNNNNATC 2 cut(s) 958, 4809
BsaHI GRCGYC 1 cut(s) 1039
BsaI GGTCTC 1 cut(s) 3026
BsaWI WCCGGW 1 cut(s) 1092
BsaXI ACNNNNNCTCC 4 cut(s) 670, 700, 3472, 3502
Bse3DI GCAATG 4 cut(s) 655, 1953, 3293, 4582
Bse8I GATNNNNATC 2 cut(s) 958, 4809
BseBI CCWGG 7 cut(s) 1593, 1860, 2413, 3597, 3765, 4482, 4533
BseCI ATCGAT 2 cut(s) 2947, 4717
BseJI GATNNNNATC 2 cut(s) 958, 4809
BseMI GCAATG 4 cut(s) 655, 1953, 3293, 4582
BseMII CTCAG 9 cut(s) 516, 729, 2021, 2700, 2834, 3293, 4535, 4907, 4968
BseRI GAGGAG 3 cut(s) 809, 1833, 3995
BseSI GKGCMC 4 cut(s) 3171, 3496, 3691, 4095
BseYI CCCAGC 2 cut(s) 845, 3134
BsgI GTGCAG 2 cut(s) 835, 2520
Bsh1236I CGCG 1 cut(s) 4955
BshNI GGYRCC 6 cut(s) 427, 706, 1038, 3168, 4394, 4844
BshVI ATCGAT 2 cut(s) 2947, 4717
BsiHKAI GWGCWC 4 cut(s) 2496, 3088, 4071, 4894
BsiHKCI CYCGRG 3 cut(s) 376, 3882, 4320
BsiSI CCGG 4 cut(s) 1093, 1263, 3841, 4321
BslFI GGGAC 6 cut(s) 1290, 2621, 3568, 3613, 4338, 4806
BsmAI GTCTC 8 cut(s) 678, 1317, 1455, 2370, 2976, 3026, 4524, 4779
BsmFI GGGAC 6 cut(s) 1290, 2621, 3568, 3613, 4338, 4806
BsmI GAATGC 7 cut(s) 817, 1024, 1894, 2207, 2312, 4158, 4729
Bso31I GGTCTC 1 cut(s) 3026
BsoBI CYCGRG 3 cut(s) 376, 3882, 4320
Bsp119I TTCGAA 3 cut(s) 1684, 2696, 3323
Bsp120I GGGCCC 2 cut(s) 3492, 4091
Bsp1286I GDGCHC 8 cut(s) 2496, 3088, 3171, 3496, 3691, 4071, 4095, 4894
Bsp1407I TGTACA 1 cut(s) 2905
Bsp1720I GCTNAGC 1 cut(s) 738
Bsp19I CCATGG 2 cut(s) 2797, 4095
BspCNI CTCAG 9 cut(s) 517, 730, 2022, 2701, 2833, 3292, 4534, 4906, 4969
BspDI ATCGAT 2 cut(s) 2947, 4717
BspFNI CGCG 1 cut(s) 4955
BspHI TCATGA 2 cut(s) 3523, 4947
BspMAI CTGCAG 1 cut(s) 2501
BspMI ACCTGC 1 cut(s) 4282
BspPI GGATC 9 cut(s) 69, 269, 836, 961, 2748, 2761, 3869, 3882, 4354
BspQI GCTCTTC 1 cut(s) 4059
BspT104I TTCGAA 3 cut(s) 1684, 2696, 3323
BspT107I GGYRCC 6 cut(s) 427, 706, 1038, 3168, 4394, 4844
BspTI CTTAAG 1 cut(s) 2564
BspTNI GGTCTC 1 cut(s) 3026
BsrBI CCGCTC 3 cut(s) 1276, 3735, 4850
BsrDI GCAATG 4 cut(s) 655, 1953, 3293, 4582
BsrGI TGTACA 1 cut(s) 2905
BssNI GRCGYC 1 cut(s) 1039
BssSI CACGAG 1 cut(s) 2481
BssT1I CCWWGG 6 cut(s) 998, 1728, 2797, 2963, 4095, 4547
Bst2BI CACGAG 1 cut(s) 2481
Bst2UI CCWGG 7 cut(s) 1593, 1860, 2413, 3597, 3765, 4482, 4533
Bst6I CTCTTC 9 cut(s) 62, 113, 164, 686, 1858, 3380, 3705, 4059, 4992
BstACI GRCGYC 1 cut(s) 1039
BstAFI CTTAAG 1 cut(s) 2564
BstAPI GCANNNNNTGC 3 cut(s) 605, 2780, 4844
BstAUI TGTACA 1 cut(s) 2905
BstBI TTCGAA 3 cut(s) 1684, 2696, 3323
BstC8I GCNNGC 6 cut(s) 2064, 2517, 3537, 4224, 4473, 4751
BstDSI CCRYGG 5 cut(s) 1847, 2797, 4095, 4935, 4953
BstENI CCTNNNNNAGG 3 cut(s) 1564, 3303, 3341
BstFNI CGCG 1 cut(s) 4955
BstH2I RGCGCY 1 cut(s) 1042
BstHHI GCGC 2 cut(s) 1041, 1967
BstMAI GTCTC 8 cut(s) 678, 1317, 1455, 2370, 2976, 3026, 4524, 4779
BstNI CCWGG 7 cut(s) 1593, 1860, 2413, 3597, 3765, 4482, 4533
BstNSI RCATGY 3 cut(s) 608, 2630, 4859
BstSFI CTRYAG 3 cut(s) 2067, 2497, 4130
BstSLI GKGCMC 4 cut(s) 3171, 3496, 3691, 4095
BstUI CGCG 1 cut(s) 4955
BstV2I GAAGAC 4 cut(s) 624, 900, 1099, 4665
BstX2I RGATCY 5 cut(s) 53, 61, 2753, 3874, 4915
BstXI CCANNNNNNTGG 3 cut(s) 285, 4185, 4430
BstYI RGATCY 5 cut(s) 53, 61, 2753, 3874, 4915
Bsu15I ATCGAT 2 cut(s) 2947, 4717
BsuI GTATCC 1 cut(s) 2866
BsuTUI ATCGAT 2 cut(s) 2947, 4717
BtgI CCRYGG 5 cut(s) 1847, 2797, 4095, 4935, 4953
BtrI CACGTC 1 cut(s) 4006
BtsI GCAGTG 5 cut(s) 1974, 3147, 3262, 3472, 3923
BveI ACCTGC 1 cut(s) 4282
Cac8I GCNNGC 6 cut(s) 2064, 2517, 3537, 4224, 4473, 4751
CciI TCATGA 2 cut(s) 3523, 4947
CfoI GCGC 2 cut(s) 1041, 1967
Cfr13I GGNCC 9 cut(s) 1799, 2409, 2608, 3260, 3492, 3493, 4091, 4092, 4932
Cfr42I CCGCGG 1 cut(s) 4956
Cfr9I CCCGGG 1 cut(s) 4320
ClaI ATCGAT 2 cut(s) 2947, 4717
CseI GACGC 1 cut(s) 1943
Csp6I GTAC 8 cut(s) 516, 1132, 2248, 2397, 2868, 2906, 3162, 3565
CviQI GTAC 8 cut(s) 516, 1132, 2248, 2397, 2868, 2906, 3162, 3565
DinI GGCGCC 1 cut(s) 1040
DraI TTTAAA 3 cut(s) 1497, 1564, 2164
EaeI YGGCCR 1 cut(s) 1982
Eam1104I CTCTTC 9 cut(s) 62, 113, 164, 686, 1858, 3380, 3705, 4059, 4992
EarI CTCTTC 9 cut(s) 62, 113, 164, 686, 1858, 3380, 3705, 4059, 4992
Ecl136II GAGCTC 3 cut(s) 2494, 4069, 4892
Eco130I CCWWGG 6 cut(s) 998, 1728, 2797, 2963, 4095, 4547
Eco147I AGGCCT 2 cut(s) 1884, 2968
Eco24I GRGCYC 5 cut(s) 2496, 3496, 4071, 4095, 4894
Eco31I GGTCTC 1 cut(s) 3026
Eco32I GATATC 1 cut(s) 4732
Eco47I GGWCC 3 cut(s) 1799, 2608, 3260
Eco53kI GAGCTC 3 cut(s) 2494, 4069, 4892
Eco57I CTGAAG 7 cut(s) 86, 951, 2244, 3726, 3966, 4074, 4157
Eco88I CYCGRG 3 cut(s) 376, 3882, 4320
EcoICRI GAGCTC 3 cut(s) 2494, 4069, 4892
EcoNI CCTNNNNNAGG 3 cut(s) 1564, 3303, 3341
EcoO109I RGGNCCY 1 cut(s) 3492
EcoRI GAATTC 5 cut(s) 259, 592, 2233, 2377, 4608
EcoRII CCWGG 7 cut(s) 1591, 1858, 2411, 3595, 3763, 4480, 4531
EcoRV GATATC 1 cut(s) 4732
EcoT14I CCWWGG 6 cut(s) 998, 1728, 2797, 2963, 4095, 4547
EcoT22I ATGCAT 2 cut(s) 3708, 4840
EcoT38I GRGCYC 5 cut(s) 2496, 3496, 4071, 4095, 4894
EgeI GGCGCC 1 cut(s) 1040
EheI GGCGCC 1 cut(s) 1040
ErhI CCWWGG 6 cut(s) 998, 1728, 2797, 2963, 4095, 4547
FalI AAGNNNNNCTT 4 cut(s) 1855, 1887, 3219, 3251
FaqI GGGAC 6 cut(s) 1290, 2621, 3568, 3613, 4338, 4806
FauI CCCGC 2 cut(s) 245, 2366
FauNDI CATATG 1 cut(s) 4024
FbaI TGATCA 3 cut(s) 472, 3517, 4027
FriOI GRGCYC 5 cut(s) 2496, 3496, 4071, 4095, 4894
FspBI CTAG 9 cut(s) 216, 398, 1251, 1619, 2252, 3405, 4328, 4686, 4919
GlaI GCGC 2 cut(s) 1040, 1966
GsaI CCCAGC 2 cut(s) 849, 3138
GsuI CTGGAG 4 cut(s) 2155, 3323, 4104, 4969
HaeII RGCGCY 1 cut(s) 1042
HapII CCGG 4 cut(s) 1093, 1263, 3841, 4321
HgaI GACGC 1 cut(s) 1943
HhaI GCGC 2 cut(s) 1041, 1967
Hin1I GRCGYC 1 cut(s) 1039
Hin6I GCGC 2 cut(s) 1039, 1965
HinP1I GCGC 2 cut(s) 1039, 1965
HincII GTYRAC 4 cut(s) 973, 1213, 2014, 2659
HindII GTYRAC 4 cut(s) 973, 1213, 2014, 2659
HindIII AAGCTT 2 cut(s) 1783, 3533
HpaII CCGG 4 cut(s) 1093, 1263, 3841, 4321
Hpy99I CGWCG 1 cut(s) 346
HpyCH4IV ACGT 2 cut(s) 621, 4005
HpySE526I ACGT 2 cut(s) 621, 4005
Hsp92I GRCGYC 1 cut(s) 1039
HspAI GCGC 2 cut(s) 1039, 1965
KasI GGCGCC 1 cut(s) 1038
Ksp22I TGATCA 3 cut(s) 472, 3517, 4027
KspI CCGCGG 1 cut(s) 4956
LguI GCTCTTC 1 cut(s) 4059
LmnI GCTCC 6 cut(s) 1562, 1655, 2623, 2731, 3157, 4219
MaeI CTAG 9 cut(s) 216, 398, 1251, 1619, 2252, 3405, 4328, 4686, 4919
MaeII ACGT 2 cut(s) 621, 4005
MbiI CCGCTC 3 cut(s) 1276, 3735, 4850
MfeI CAATTG 1 cut(s) 3801
MflI RGATCY 5 cut(s) 53, 61, 2753, 3874, 4915
MhlI GDGCHC 8 cut(s) 2496, 3088, 3171, 3496, 3691, 4071, 4095, 4894
MlsI TGGCCA 1 cut(s) 1984
MluNI TGGCCA 1 cut(s) 1984
Mly113I GGCGCC 1 cut(s) 1039
MmeI TCCRAC 4 cut(s) 2005, 3194, 4189, 4779
Mox20I TGGCCA 1 cut(s) 1984
Mph1103I ATGCAT 2 cut(s) 3708, 4840
MroXI GAANNNNTTC 3 cut(s) 621, 1716, 3184
MscI TGGCCA 1 cut(s) 1984
MslI CAYNNNNRTG 5 cut(s) 1047, 4100, 4392, 4491, 4568
Msp20I TGGCCA 1 cut(s) 1984
MspA1I CMGCKG 2 cut(s) 849, 4955
MspCI CTTAAG 1 cut(s) 2564
MspI CCGG 4 cut(s) 1093, 1263, 3841, 4321
MunI CAATTG 1 cut(s) 3801
Mva1269I GAATGC 7 cut(s) 817, 1024, 1894, 2207, 2312, 4158, 4729
MvaI CCWGG 7 cut(s) 1593, 1860, 2413, 3597, 3765, 4482, 4533
MvnI CGCG 1 cut(s) 4955
NarI GGCGCC 1 cut(s) 1039
NciI CCSGG 3 cut(s) 1263, 4321, 4322
NcoI CCATGG 2 cut(s) 2797, 4095
NdeI CATATG 1 cut(s) 4024
NmeAIII GCCGAG 1 cut(s) 160
NmuCI GTSAC 8 cut(s) 1196, 1550, 1761, 2352, 2639, 3467, 4359, 4988
NsiI ATGCAT 2 cut(s) 3708, 4840
NspI RCATGY 3 cut(s) 608, 2630, 4859
NspV TTCGAA 3 cut(s) 1684, 2696, 3323
PagI TCATGA 2 cut(s) 3523, 4947
PceI AGGCCT 2 cut(s) 1884, 2968
PciSI GCTCTTC 1 cut(s) 4059
PcsI WCGNNNNNNNCGW 1 cut(s) 682
PctI GAATGC 7 cut(s) 817, 1024, 1894, 2207, 2312, 4158, 4729
PdmI GAANNNNTTC 3 cut(s) 621, 1716, 3184
PflFI GACNNNGTC 1 cut(s) 2812
PluTI GGCGCC 1 cut(s) 1042
PshBI ATTAAT 3 cut(s) 1146, 1905, 2685
Psp124BI GAGCTC 3 cut(s) 2496, 4071, 4894
Psp6I CCWGG 7 cut(s) 1591, 1858, 2411, 3595, 3763, 4480, 4531
PspFI CCCAGC 2 cut(s) 845, 3134
PspGI CCWGG 7 cut(s) 1591, 1858, 2411, 3595, 3763, 4480, 4531
PspOMI GGGCCC 2 cut(s) 3492, 4091
PspPI GGNCC 9 cut(s) 1799, 2409, 2608, 3260, 3492, 3493, 4091, 4092, 4932
PstI CTGCAG 1 cut(s) 2501
PsuI RGATCY 5 cut(s) 53, 61, 2753, 3874, 4915
PsyI GACNNNGTC 1 cut(s) 2812
PvuII CAGCTG 1 cut(s) 849
RsaI GTAC 8 cut(s) 517, 1133, 2249, 2398, 2869, 2907, 3163, 3566
RsaNI GTAC 8 cut(s) 516, 1132, 2248, 2397, 2868, 2906, 3162, 3565
RseI CAYNNNNRTG 5 cut(s) 1047, 4100, 4392, 4491, 4568
SacI GAGCTC 3 cut(s) 2496, 4071, 4894
SacII CCGCGG 1 cut(s) 4956
SapI GCTCTTC 1 cut(s) 4059
Sau96I GGNCC 9 cut(s) 1799, 2409, 2608, 3260, 3492, 3493, 4091, 4092, 4932
ScaI AGTACT 1 cut(s) 3566
SduI GDGCHC 8 cut(s) 2496, 3088, 3171, 3496, 3691, 4071, 4095, 4894
SfcI CTRYAG 3 cut(s) 2067, 2497, 4130
SfoI GGCGCC 1 cut(s) 1040
Sfr303I CCGCGG 1 cut(s) 4956
SfuI TTCGAA 3 cut(s) 1684, 2696, 3323
SgrBI CCGCGG 1 cut(s) 4956
SinI GGWCC 3 cut(s) 1799, 2608, 3260
SmaI CCCGGG 1 cut(s) 4322
SmiMI CAYNNNNRTG 5 cut(s) 1047, 4100, 4392, 4491, 4568
SmlI CTYRAG 2 cut(s) 2564, 4877
SmoI CTYRAG 2 cut(s) 2564, 4877
SpeI ACTAGT 1 cut(s) 215
SseBI AGGCCT 2 cut(s) 1884, 2968
SspDI GGCGCC 1 cut(s) 1038
SspMI CTAG 9 cut(s) 216, 398, 1251, 1619, 2252, 3405, 4328, 4686, 4919
SstI GAGCTC 3 cut(s) 2496, 4071, 4894
StuI AGGCCT 2 cut(s) 1884, 2968
StyI CCWWGG 6 cut(s) 998, 1728, 2797, 2963, 4095, 4547
TaiI ACGT 2 cut(s) 624, 4008
TaqII GACCGA 1 cut(s) 3063
TatI WGTACW 3 cut(s) 2905, 3161, 3564
TauI GCSGC 3 cut(s) 1693, 1946, 4850
TseFI GTSAC 8 cut(s) 1196, 1550, 1761, 2352, 2639, 3467, 4359, 4988
Tsp45I GTSAC 8 cut(s) 1196, 1550, 1761, 2352, 2639, 3467, 4359, 4988
TspGWI ACGGA 3 cut(s) 1045, 1864, 3233
TspMI CCCGGG 1 cut(s) 4320
Tth111I GACNNNGTC 1 cut(s) 2812
Vha464I CTTAAG 1 cut(s) 2564
VpaK11BI GGWCC 3 cut(s) 1799, 2608, 3260
VspI ATTAAT 3 cut(s) 1146, 1905, 2685
XagI CCTNNNNNAGG 3 cut(s) 1564, 3303, 3341
XbaI TCTAGA 1 cut(s) 3404
XceI RCATGY 3 cut(s) 608, 2630, 4859
XcmI CCANNNNNNNNNTGG 1 cut(s) 3248
XmaI CCCGGG 1 cut(s) 4320
XmnI GAANNNNTTC 3 cut(s) 621, 1716, 3184
XspI CTAG 9 cut(s) 216, 398, 1251, 1619, 2252, 3405, 4328, 4686, 4919
ZrmI AGTACT 1 cut(s) 3566
Zsp2I ATGCAT 2 cut(s) 3708, 4840
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.