Rh6BG526900

Enhancer of polycomb-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0010036
Physical Location & Seq
Reverse (-)
71838349 .. 71845672
7324 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG526900.1

Sequence Viewer

Length: 4944 bp
ATGGAAAATAGAATAGAGGTTTCACATGGCACTGAAATTCCCAGGAAATCGAGATCTTTGGATCTTAAGAGTTTGTACAAGTCCAGGAGTAGAAAAGAGGCTGAAAATCAGAGCCTGAAGAGAAAAGGGAGTGAGGACGACGACGACGGTGGGGACAAGAAGAAGAGGAGTAGGAAAGAGGTGTCTCTCAGTAGTCTCAAGAATGTTAATACTAGTAGTAGCAAGAAGAGTATAGATAAAGTTTATCATAGAGGGTTGGATTCTGGTTCACATGATCCGGACGCATTCAAATCGGGGTCAAGTCAAAAATTGGATAGCAGTAGTGGGTTAAATGGCGTTTCAAAGCTTAGTTTGGATAATAGTGGCATTCAAATCCCGAGGCGTAAGAGGGGATTCGTGGGGCGAAAAAAATGTGAGGGTGCTCAGCTGTTGAAGCTGTCTGATGAGTCTGCTGGTAGAGCAACTAGTGTTGATCAGAATCATCAGATAGCTAAGTTAAGTGGTGATGATTTGGGTACTCAAGCTGAGGCCTGGAAGGCCATAAGAAACAAAGGTTTGGATGAATGCAAAGAAAATATAAATAGCGAGTTGAATGCAACTTCACATGCAAAGAAAGAAAATGTTCTTGAGAGGCAGTCAGTTGCAAGCAATGGTAATTCATCTTTGAAAAAGTCCCGGAGGAATCGTTGGAAAAGGAAGGACTTGTCACCTGATAGTAAAACTGATGCAAAGGAAGCTGAGCCTTTAGTCAATAGTTCTACTAAAGCATGCCAGACTTCACAAGAAGACGAGGAGGAGAATCTTGAAGAGAATGCAGCGAGGATGCTTTCATCGAGGTTTGATCCAAGCTGTACTGGGTTTTCATTAAACACCAAGGCTTCTGCGATGCAATCTTCAAACGGGTTGTCTAATCAGTCATCTCCTGGTCAGGATTTTGATAGTCACATGTCGAAGTCATTTTCTGGATCAGAATCTCAATCTGTTGATAATGCTGGTAGAGTGCTGAGGCCCAGGGCCAGGAAGCAGCACAAAGAAAAGAAAGGTACGAGGAAGAGGCGCCACTTCTATGAAATCTTCTTTGGGGACTTGGATGCTTGTTGGGTATTGAACCGTAGGATCAAGGTCTTCTGGCCTTTGGACCAAAGTTGGTATTATGGCCTTGTGAATGATTATGACAAGGAGAAAAAACTTCACCATATTAGATACGATGACCGTGAAGAAGAATGGGTTGATCTTCAACATGAGAGGTTCAAGCTCTTGCTACTTCCTACTGAAGTTCCTGGTAAGGCAAAGCAGAAAAAATCATTCATGCAAATTACAAGTTCTGAAGAGAGAGAAGGAAACTTGAAACCCAGAAAAGAGAAGAAAAAGAGAGACTCGATGTCGGAGGACGATAGCTGCATAGGCAGCTGTATGGATTCTGAACCTATTATTTCATGGTTGTCGCGATCTACCCGTCGGATCAAGTCTCCTTCTCATGCTGTGAAGAAGCAAAAAACAACTGGTCCATCTCCAAAGTCCGTGCCAACTTTGTCTGACAGTACTGGCACTTTACATGGGTGTTTGAGTGATGTTTCCTCTAGAAGGGATACAAATAAATTATCTTGTAATTCCGGCAGATCATCTGATCCTGTGAGGGAAGAGAAGCCTGCTCCAGAAGGCAACTCCGAAGATAGCAAAATGCCTATTGTTTATTATCGGAAGCGATTGCGCAAGACTGGCTCTGTACTGTCTCAAATATACAAGGATGAGCATGCTTCCATGCATGGACATCATTGTTGTACTTCTGTTACTCCTGTTGAGGAGGTTTGGGACTTGGAAGAGCCAGATGATTTTGTTGTTATATTGGATCGCAGTTGGCCATTGTGGTATAGTGATGATGCAGGGTTGCTAAAGTTAACTCTTCCTTGGATAGAATCAGGAAAAGTCAAATTTAAATGCCTTCCACTGCATTCACTAATGAATGATTCTCTTGGAGTAGAGTTGTTACGGTTCTTTCATGCTGCAATGCTGCTTCGGCATGGTGTAGTGGTGACCACATGGCCAAAGGTTCATTTGGAGATGCTCTTTGTTGATAATGTAGTTGGACTGAGGTTTCTTTTGTTTGAAGGTTGCTTGAAGCAGGCGGTGGCCTTTGTTTCTCTTATCCTGACATTATTTCATCACCCTAATGAACAGGGGAAGCTTACTGATTTCCAGTTACCAGCAACTTCAATCAGGTTTAAATTCTCCTGTGTTCAGCATCTTGGAAAGCAACTTGTGTCTGCTTTCTACAACTTCTGTCAAGTTAAAAATTCAACATGGATGTACCTAGACAATAAACTCAGGAGGCATTGTTTACTCGCCAAAAAACTACCTCTGTCTGAATGCACCTATGATAATATCCAGGCACTTCAAAATGGAATAAATCAGTCACCTTCTATGTCTCTTTGCGGGCAACCCTCTGTCAAGGGTACACGGAAGAGGTCCAGGCAGGGCATTAACTTCATGGGCAGTTCTCGGGAAGTTAGTTTTGTAAATATCAGCCATTCTGCTACTCATTCTGATGAGCTTCACAGAAAGCTTCCCCCACTTGCTCTATCTTTTACAGCTGCACCTACTTTCTTCATTAATTTGCATCTTAAGTTGCTTATGGAACACAGTGTTGCTAATATCTGCTTTCAAGACCATGATTCAGAAATTACTCCCGAGAACAATTTGAAGGCTCCAGAAAATGTGGCTACCTCTGGTGGGTCCTGTACTAAAGTGGTAACAGAAACATCTCTTTCTATTTGCAGTCAGCGCGGTCGGTTAAAGTCATCTCAGCTTTACCAAAATTGTGTTGTTAATGTTGCTGGAGCCTCTTCTAGGACTTTCGCTGGCAGAGATAAATCCGAAACTAGTTCCCGTTCTATTGTGAATGGGCTTACTGTTGAAATTCCATCTTTTGACCATTTTGAGAAGCCTGTGGAGAGGGAGTTACAAAGTGCTCAACAGCCTACAGATTTTCAACAGCCTACAGTTTTTTCATTGAATATGAATGGTAGCATTATCCCCAGTCCCAGCCCCACTGCTCCCAGAAGTACAGGGCAGCGAAATAGAAATAATATGTCATCATTTGGAAACCTCGCACATCGTTGGTCAGATGGAAAGGCTGACATTTTCCATAATGGTTTTGGTAATGGACCGAAGAAGCCCCGAACTCAAGTCTCATACACATTGCCTTGTGGAGGTTTTGATGTCAGCTCTAAGCAAAGAAATGTTCACAAAGGGCTTCCTAATAAGCGAATTAGAAGGGCCAGCGAGAAGAGGTCATCAGACATTTCCAGAGGTTCCGAAAGGAACTTGGAGCTCTTATCCTGTGAGGCAAATGTGTTAATTTCTGCAAGTGACAGAGGGTGGAGAGAATGTGGGGCACGTGTAGTATTAGAACTCTTTGATAATAGTGAGTGGAAGCTTGCTGTAAAACTTTCAGGGACTACGAAGTATTCATACAAAGCACATCAATTTTTGCAGCCTGGGTCAACAAACCGCTATACTCATGTTATGATGTGGAAAGGAGGAAAGGATTGGATCTTGGAGTTTCCAGATAGGAGCCAGTGGGCCCTTTTCAAGGAGATGCATGAAGAGTGTTACAATCGGAACCTTCGGTCTGCGTCAGTTAAAAACATACCTATTCCTGGTGTTCGCTTAGTAGAAGATATTGATGATAATGGAACAGAAATAGCATTTCTTCGCAGTTCTACCAAGTACTTTCAGCAGATGAAAACCGATGTTGAGATGGCTTTAGATCCATCCCGTCTCTTGTATGACATGGATAGTGATGATGAGCGATGGATTTTGAAGTTTCAAAATTCTTCAGAAGTTGAAAAGAGTAGCTCAATGGAGATTGGCGAGGAGATGTTTGAGAAGACAATGGACATGTTTGAGAAGGCTGCATATGTTCGGCAATGTGATCAGTTCACATCTGAAGAAATAGAAGAGTTCATGACTGGTATTGGGCCCATGGATTTGATTAAAACCATTTATGAGCATTGGCGTCAGAAACGGCTGAGAAAGGGAATGCCTTTAATCCGACATCTCCAGCCACCGTCATGGGAGACGTATCAAAAACAAGTGAGGGAGTGGGAGCAAGCTATGACCAAGATGAATAACACACTCGCAAATGGTGGCCGTGAGAAAGCTGCAGCAGTTGAGAAGCCACCGATGTATGCTTTTTGTCTGAAACCACGGGGCCTGGAAGTTCCCAATAAGGGATCAAAGCAACGGTCACAGAAGAAATTTTCTATTTCTGCACATACCAATGCGGTGTTAGGAGATCAGGATGGTTTCCATCCTATTGGAAGGAGATCAAATGGGTTTGCCTATGGTGATGAAAAAATTGCATATTCAGGCCATAACTATGAATCTTTAGATGACTCTCCATTGTCTCAGACGTCGCCCAGGGTTTTTTCACCAAGGGATGCAGCAAACATCTTTATGAGCAATGATGGTTTTGAGAGGAATCATCTTCACAGAATTGACAGAAGCAAGTCAAAGAAATATCGGACAATAGCATCCTCTGTTGACCCCCAGATGGTGTCTCCATATAATCATAGAGTGGTACGAAACAGAAATGGAGTTCATAGAGGGAATATTGGTTTCCCGGAGTGGTCAAGCCAGTCATATTATCAACCAGATGTTGCTCAGAGGCTTGTAAATGCGCATGGCTTTGATCATGATGAGTTTAGGCTTCGTGAGGCATCCGGTGCTGCTCAGCATGCACATAACTTAGCCAAGCGTAAGAGAGAAAATGCTCGCAGATTGTTTTACAGAGCAGATCTAGCAATGCACAAGGCTGTGGTTGCTCTCATGACTGCGGAAGCAATCAAAGCTTCTTCAGAGGACTACGACTATGACTACGACTCTGAGTCCGACTCCTTCTCCATCTCCGACTCTGACACCGATTCAGACAGTGAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPL1

Protein Analysis

1647

Amino Acids

186.48

Weight (kDa)

9.08

Isoelectric Point (pI)

58.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPL1 PF10513 1209 - 1304 1.7e-08 Enhancer of polycomb-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 4418
Acc16I TGCGCA 2 cut(s) 1712, 4683
AccB1I GGYRCC 1 cut(s) 1056
AccII CGCG 2 cut(s) 1447, 2784
AccIII TCCGGA 1 cut(s) 277
AciI CCGC 6 cut(s) 2126, 2433, 2784, 3511, 4286, 4838
AcoI YGGCCR 3 cut(s) 1859, 2042, 4150
AcsI RAATTY 7 cut(s) 36, 1931, 2225, 2293, 2916, 3832, 4259
AcuI CTGAAG 6 cut(s) 137, 1293, 1347, 3822, 3969, 4842
AcvI CACGTG 1 cut(s) 3398
AcyI GRCGYC 3 cut(s) 1057, 4018, 4415
AfiI CCNNNNNNNGG 9 cut(s) 1017, 1584, 2730, 2847, 3209, 3592, 3659, 4232, 4555
AflII CTTAAG 2 cut(s) 65, 2621
AflIII ACRYGT 3 cut(s) 945, 3397, 3900
AhdI GACNNNNNGTC 2 cut(s) 1381, 4888
AhlI ACTAGT 3 cut(s) 212, 464, 2879
AjuI GAANNNNNNNTTGG 2 cut(s) 1062, 1094
Alw21I GWGCWC 3 cut(s) 424, 2971, 3333
AlwNI CAGNNNCTG 1 cut(s) 115
Ama87I CYCGRG 3 cut(s) 376, 2499, 2687
Aor13HI TCCGGA 1 cut(s) 277
ApaI GGGCCC 2 cut(s) 3586, 3984
ApoI RAATTY 7 cut(s) 36, 1931, 2225, 2293, 2916, 3832, 4259
ArsI GACNNNNNNTTYG 4 cut(s) 890, 922, 4232, 4264
AseI ATTAAT 1 cut(s) 2610
Asp700I GAANNNNTTC 1 cut(s) 621
AspLEI GCGC 4 cut(s) 1059, 1713, 2784, 4684
AsuC2I CCSGG 2 cut(s) 676, 4625
AsuHPI GGTGA 8 cut(s) 515, 699, 1184, 2044, 2156, 2406, 4361, 4425
AvaI CYCGRG 3 cut(s) 376, 2499, 2687
AvaII GGWCC 5 cut(s) 1138, 1505, 2466, 2733, 3164
BaeGI GKGCMC 3 cut(s) 3397, 3586, 3984
BalI TGGCCA 2 cut(s) 1861, 2044
BanI GGYRCC 1 cut(s) 1056
BanII GRGCYC 3 cut(s) 3333, 3586, 3984
BarI GAAGNNNNNNTAC 2 cut(s) 3455, 3487
BbrPI CACGTG 1 cut(s) 3398
BbsI GAAGAC 3 cut(s) 792, 1117, 3896
Bbv12I GWGCWC 3 cut(s) 424, 2971, 3333
BbvCI CCTCAGC 2 cut(s) 525, 1004
BceAI ACGGC 2 cut(s) 4043, 4137
BcgI CGANNNNNNTGC 4 cut(s) 273, 307, 575, 609
BciVI GTATCC 1 cut(s) 1582
BclI TGATCA 3 cut(s) 472, 3934, 4693
BcnI CCSGG 2 cut(s) 676, 4625
BcuI ACTAGT 3 cut(s) 212, 464, 2879
BfaI CTAG 7 cut(s) 213, 465, 1581, 2312, 2847, 2880, 4802
BfmI CTRYAG 3 cut(s) 2979, 2997, 4164
BfoI RGCGCY 1 cut(s) 1060
BfrI CTTAAG 2 cut(s) 65, 2621
BfuI GTATCC 1 cut(s) 1582
BglI GCCNNNNNGGC 1 cut(s) 536
BglII AGATCT 2 cut(s) 53, 4798
BlpI GCTNAGC 3 cut(s) 423, 738, 4734
BmcAI AGTACT 2 cut(s) 1543, 3731
Bme18I GGWCC 5 cut(s) 1138, 1505, 2466, 2733, 3164
BmeRI GACNNNNNGTC 2 cut(s) 1381, 4888
BmeT110I CYCGRG 3 cut(s) 376, 2499, 2687
BmrI ACTGGG 2 cut(s) 864, 3030
BmuI ACTGGG 2 cut(s) 864, 3030
BpiI GAAGAC 3 cut(s) 792, 1117, 3896
BplI GAGNNNNNCTC 2 cut(s) 4880, 4912
BpmI CTGGAG 4 cut(s) 1638, 2691, 2856, 4046
Bpu10I CCTNAGC 2 cut(s) 525, 1004
Bpu1102I GCTNAGC 3 cut(s) 423, 738, 4734
BpuEI CTTGAG 4 cut(s) 182, 504, 647, 3168
BpuMI CCSGG 2 cut(s) 676, 4625
BsaAI YACGTR 1 cut(s) 3398
BsaBI GATNNNNATC 2 cut(s) 477, 970
BsaHI GRCGYC 3 cut(s) 1057, 4018, 4415
BsaWI WCCGGW 2 cut(s) 277, 4724
Bsc4I CCNNNNNNNGG 9 cut(s) 1017, 1584, 2730, 2847, 3209, 3592, 3659, 4232, 4555
Bse1I ACTGG 9 cut(s) 859, 1507, 1549, 1723, 2197, 3036, 3577, 3976, 4639
Bse3DI GCAATG 6 cut(s) 655, 2013, 3197, 3935, 4471, 4812
Bse8I GATNNNNATC 2 cut(s) 477, 970
BseAI TCCGGA 1 cut(s) 277
BseJI GATNNNNATC 2 cut(s) 477, 970
BseLI CCNNNNNNNGG 9 cut(s) 1017, 1584, 2730, 2847, 3209, 3592, 3659, 4232, 4555
BseMI GCAATG 6 cut(s) 655, 2013, 3197, 3935, 4471, 4812
BseNI ACTGG 9 cut(s) 859, 1507, 1549, 1723, 2197, 3036, 3577, 3976, 4639
BseRI GAGGAG 5 cut(s) 181, 806, 809, 1817, 3890
BseSI GKGCMC 3 cut(s) 3397, 3586, 3984
BseYI CCCAGC 1 cut(s) 3041
BsgI GTGCAG 2 cut(s) 2577, 4257
Bsh1236I CGCG 2 cut(s) 1447, 2784
Bsh1285I CGRYCG 1 cut(s) 2788
BshNI GGYRCC 1 cut(s) 1056
BsiEI CGRYCG 1 cut(s) 2788
BsiHKAI GWGCWC 3 cut(s) 424, 2971, 3333
BsiHKCI CYCGRG 3 cut(s) 376, 2499, 2687
BsiSI CCGG 5 cut(s) 278, 676, 1614, 4625, 4725
BslFI GGGAC 6 cut(s) 167, 658, 1097, 1826, 3024, 3469
BslI CCNNNNNNNGG 9 cut(s) 1017, 1584, 2730, 2847, 3209, 3592, 3659, 4232, 4555
BsmBI CGTCTC 2 cut(s) 3785, 4073
BsmFI GGGAC 6 cut(s) 167, 658, 1097, 1826, 3024, 3469
BsmI GAATGC 8 cut(s) 284, 366, 569, 598, 817, 1951, 2372, 4047
BsoBI CYCGRG 3 cut(s) 376, 2499, 2687
Bsp120I GGGCCC 2 cut(s) 3582, 3980
Bsp1286I GDGCHC 6 cut(s) 424, 2971, 3333, 3397, 3586, 3984
Bsp13I TCCGGA 1 cut(s) 277
Bsp1407I TGTACA 1 cut(s) 75
Bsp1720I GCTNAGC 3 cut(s) 423, 738, 4734
Bsp19I CCATGG 1 cut(s) 3984
Bsp68I TCGCGA 1 cut(s) 1447
BspACI CCGC 6 cut(s) 2126, 2433, 2784, 3511, 4286, 4838
BspEI TCCGGA 1 cut(s) 277
BspFNI CGCG 2 cut(s) 1447, 2784
BspHI TCATGA 3 cut(s) 3966, 4696, 4830
BspMAI CTGCAG 1 cut(s) 4168
BspQI GCTCTTC 1 cut(s) 1815
BspT107I GGYRCC 1 cut(s) 1056
BspTI CTTAAG 2 cut(s) 65, 2621
BsrDI GCAATG 6 cut(s) 655, 2013, 3197, 3935, 4471, 4812
BsrGI TGTACA 1 cut(s) 75
BsrI ACTGG 9 cut(s) 859, 1507, 1549, 1723, 2197, 3036, 3577, 3976, 4639
BssNI GRCGYC 3 cut(s) 1057, 4018, 4415
BssT1I CCWWGG 4 cut(s) 873, 1907, 3984, 4436
BstACI GRCGYC 3 cut(s) 1057, 4018, 4415
BstAFI CTTAAG 2 cut(s) 65, 2621
BstAPI GCANNNNNTGC 1 cut(s) 4727
BstAUI TGTACA 1 cut(s) 75
BstBAI YACGTR 1 cut(s) 3398
BstDSI CCRYGG 2 cut(s) 3984, 4208
BstEII GGTNACC 1 cut(s) 2032
BstENI CCTNNNNNAGG 4 cut(s) 1582, 2845, 3207, 3590
BstFNI CGCG 2 cut(s) 1447, 2784
BstH2I RGCGCY 1 cut(s) 1060
BstHHI GCGC 4 cut(s) 1059, 1713, 2784, 4684
BstMCI CGRYCG 1 cut(s) 2788
BstNSI RCATGY 6 cut(s) 608, 771, 949, 1757, 3904, 4742
BstPI GGTNACC 1 cut(s) 2032
BstSFI CTRYAG 3 cut(s) 2979, 2997, 4164
BstSLI GKGCMC 3 cut(s) 3397, 3586, 3984
BstUI CGCG 2 cut(s) 1447, 2784
BstV2I GAAGAC 3 cut(s) 792, 1117, 3896
BstX2I RGATCY 5 cut(s) 53, 61, 3552, 3769, 4798
BstXI CCANNNNNNTGG 2 cut(s) 4074, 4319
BstYI RGATCY 5 cut(s) 53, 61, 3552, 3769, 4798
BsuI GTATCC 1 cut(s) 1582
BtgI CCRYGG 2 cut(s) 3984, 4208
BtgZI GCGATG 2 cut(s) 899, 3826
BtsI GCAGTG 2 cut(s) 1946, 3048
BtsIMutI CAGTG 6 cut(s) 30, 1946, 2647, 3048, 3584, 4939
BtuMI TCGCGA 1 cut(s) 1447
CaiI CAGNNNCTG 1 cut(s) 115
CciI TCATGA 3 cut(s) 3966, 4696, 4830
CfoI GCGC 4 cut(s) 1059, 1713, 2784, 4684
CseI GACGC 3 cut(s) 290, 3624, 4007
DinI GGCGCC 1 cut(s) 1058
DraI TTTAAA 2 cut(s) 1936, 2224
DriI GACNNNNNGTC 2 cut(s) 1381, 4888
EaeI YGGCCR 3 cut(s) 1859, 2042, 4150
Eam1105I GACNNNNNGTC 2 cut(s) 1381, 4888
Ecl136II GAGCTC 1 cut(s) 3331
Eco130I CCWWGG 4 cut(s) 873, 1907, 3984, 4436
Eco147I AGGCCT 1 cut(s) 530
Eco24I GRGCYC 3 cut(s) 3333, 3586, 3984
Eco47I GGWCC 5 cut(s) 1138, 1505, 2466, 2733, 3164
Eco53kI GAGCTC 1 cut(s) 3331
Eco57I CTGAAG 6 cut(s) 137, 1293, 1347, 3822, 3969, 4842
Eco72I CACGTG 1 cut(s) 3398
Eco88I CYCGRG 3 cut(s) 376, 2499, 2687
Eco91I GGTNACC 1 cut(s) 2032
EcoICRI GAGCTC 1 cut(s) 3331
EcoNI CCTNNNNNAGG 4 cut(s) 1582, 2845, 3207, 3590
EcoO109I RGGNCCY 3 cut(s) 2733, 3583, 4213
EcoO65I GGTNACC 1 cut(s) 2032
EcoT14I CCWWGG 4 cut(s) 873, 1907, 3984, 4436
EcoT22I ATGCAT 2 cut(s) 1767, 3603
EcoT38I GRGCYC 3 cut(s) 3333, 3586, 3984
EgeI GGCGCC 1 cut(s) 1058
EheI GGCGCC 1 cut(s) 1058
ErhI CCWWGG 4 cut(s) 873, 1907, 3984, 4436
Esp3I CGTCTC 2 cut(s) 3785, 4073
FaqI GGGAC 6 cut(s) 167, 658, 1097, 1826, 3024, 3469
FauI CCCGC 1 cut(s) 2426
FauNDI CATATG 1 cut(s) 3919
FbaI TGATCA 3 cut(s) 472, 3934, 4693
FriOI GRGCYC 3 cut(s) 3333, 3586, 3984
FspAI RTGCGCAY 1 cut(s) 4683
FspBI CTAG 7 cut(s) 213, 465, 1581, 2312, 2847, 2880, 4802
FspI TGCGCA 2 cut(s) 1712, 4683
GlaI GCGC 4 cut(s) 1058, 1712, 2783, 4683
GsaI CCCAGC 1 cut(s) 3045
GsuI CTGGAG 4 cut(s) 1638, 2691, 2856, 4046
HaeII RGCGCY 1 cut(s) 1060
HapII CCGG 5 cut(s) 278, 676, 1614, 4625, 4725
HgaI GACGC 3 cut(s) 290, 3624, 4007
HhaI GCGC 4 cut(s) 1059, 1713, 2784, 4684
Hin1I GRCGYC 3 cut(s) 1057, 4018, 4415
Hin6I GCGC 4 cut(s) 1057, 1711, 2782, 4682
HinP1I GCGC 4 cut(s) 1057, 1711, 2782, 4682
HincII GTYRAC 3 cut(s) 1899, 3504, 4546
HindII GTYRAC 3 cut(s) 1899, 3504, 4546
HindIII AAGCTT 5 cut(s) 344, 2183, 2561, 3434, 4851
HpaI GTTAAC 1 cut(s) 1899
HpaII CCGG 5 cut(s) 278, 676, 1614, 4625, 4725
HphI GGTGA 8 cut(s) 515, 699, 1184, 2044, 2156, 2406, 4361, 4425
Hpy166II GTNNAC 8 cut(s) 269, 1899, 2339, 2456, 3244, 3504, 3942, 4546
Hpy8I GTNNAC 8 cut(s) 269, 1899, 2339, 2456, 3244, 3504, 3942, 4546
Hpy99I CGWCG 5 cut(s) 143, 146, 149, 1461, 4420
HpyCH4IV ACGT 3 cut(s) 3397, 4082, 4415
HpySE526I ACGT 3 cut(s) 3397, 4082, 4415
Hsp92I GRCGYC 3 cut(s) 1057, 4018, 4415
HspAI GCGC 4 cut(s) 1057, 1711, 2782, 4682
KasI GGCGCC 1 cut(s) 1056
Kpn2I TCCGGA 1 cut(s) 277
Ksp22I TGATCA 3 cut(s) 472, 3934, 4693
KspAI GTTAAC 1 cut(s) 1899
LguI GCTCTTC 1 cut(s) 1815
LmnI GCTCC 7 cut(s) 1657, 2710, 2837, 3058, 3328, 3573, 4108
MaeI CTAG 7 cut(s) 213, 465, 1581, 2312, 2847, 2880, 4802
MaeII ACGT 3 cut(s) 3397, 4082, 4415
MflI RGATCY 5 cut(s) 53, 61, 3552, 3769, 4798
MhlI GDGCHC 6 cut(s) 424, 2971, 3333, 3397, 3586, 3984
MlsI TGGCCA 2 cut(s) 1861, 2044
MluNI TGGCCA 2 cut(s) 1861, 2044
Mly113I GGCGCC 1 cut(s) 1057
MlyI GAGTC 7 cut(s) 455, 1370, 4391, 4877, 4889, 4898, 4907
MmeI TCCRAC 8 cut(s) 237, 668, 1365, 1439, 2065, 4078, 4917, 4935
Mox20I TGGCCA 2 cut(s) 1861, 2044
Mph1103I ATGCAT 2 cut(s) 1767, 3603
MroI TCCGGA 1 cut(s) 277
MroXI GAANNNNTTC 1 cut(s) 621
MscI TGGCCA 2 cut(s) 1861, 2044
MslI CAYNNNNRTG 7 cut(s) 1065, 2169, 2544, 4072, 4281, 4380, 4457
Msp20I TGGCCA 2 cut(s) 1861, 2044
MspA1I CMGCKG 3 cut(s) 427, 1410, 2591
MspCI CTTAAG 2 cut(s) 65, 2621
MspI CCGG 5 cut(s) 278, 676, 1614, 4625, 4725
Mva1269I GAATGC 8 cut(s) 284, 366, 569, 598, 817, 1951, 2372, 4047
MvnI CGCG 2 cut(s) 1447, 2784
NarI GGCGCC 1 cut(s) 1057
NciI CCSGG 2 cut(s) 676, 4625
NcoI CCATGG 1 cut(s) 3984
NdeI CATATG 1 cut(s) 3919
NmuCI GTSAC 6 cut(s) 705, 941, 2032, 2412, 3368, 4248
NruI TCGCGA 1 cut(s) 1447
NsbI TGCGCA 2 cut(s) 1712, 4683
NsiI ATGCAT 2 cut(s) 1767, 3603
NspI RCATGY 6 cut(s) 608, 771, 949, 1757, 3904, 4742
PaeI GCATGC 3 cut(s) 771, 1757, 4742
PagI TCATGA 3 cut(s) 3966, 4696, 4830
PasI CCCWGGG 2 cut(s) 1011, 4422
PceI AGGCCT 1 cut(s) 530
PciI ACATGT 2 cut(s) 945, 3900
PciSI GCTCTTC 1 cut(s) 1815
PctI GAATGC 8 cut(s) 284, 366, 569, 598, 817, 1951, 2372, 4047
PdmI GAANNNNTTC 1 cut(s) 621
PfoI TCCNGGA 3 cut(s) 83, 674, 4623
PleI GAGTC 7 cut(s) 454, 1370, 4391, 4877, 4889, 4897, 4907
PluTI GGCGCC 1 cut(s) 1060
PmaCI CACGTG 1 cut(s) 3398
PmlI CACGTG 1 cut(s) 3398
PpsI GAGTC 7 cut(s) 454, 1370, 4391, 4877, 4889, 4897, 4907
Ppu21I YACGTR 1 cut(s) 3398
PpuMI RGGWCCY 1 cut(s) 2733
PscI ACATGT 2 cut(s) 945, 3900
PshBI ATTAAT 1 cut(s) 2610
Psp124BI GAGCTC 1 cut(s) 3333
Psp5II RGGWCCY 1 cut(s) 2733
PspCI CACGTG 1 cut(s) 3398
PspEI GGTNACC 1 cut(s) 2032
PspFI CCCAGC 1 cut(s) 3041
PspOMI GGGCCC 2 cut(s) 3582, 3980
PspPPI RGGWCCY 1 cut(s) 2733
PstI CTGCAG 1 cut(s) 4168
PstNI CAGNNNCTG 1 cut(s) 115
PsuI RGATCY 5 cut(s) 53, 61, 3552, 3769, 4798
PvuII CAGCTG 3 cut(s) 427, 1410, 2591
RruI TCGCGA 1 cut(s) 1447
RseI CAYNNNNRTG 7 cut(s) 1065, 2169, 2544, 4072, 4281, 4380, 4457
SacI GAGCTC 1 cut(s) 3333
SapI GCTCTTC 1 cut(s) 1815
ScaI AGTACT 2 cut(s) 1543, 3731
SchI GAGTC 7 cut(s) 455, 1370, 4391, 4877, 4889, 4898, 4907
SduI GDGCHC 6 cut(s) 424, 2971, 3333, 3397, 3586, 3984
SfcI CTRYAG 3 cut(s) 2979, 2997, 4164
SfiI GGCCNNNNNGGCC 1 cut(s) 536
SfoI GGCGCC 1 cut(s) 1058
SinI GGWCC 5 cut(s) 1138, 1505, 2466, 2733, 3164
SmiI ATTTAAAT 1 cut(s) 1936
SmiMI CAYNNNNRTG 7 cut(s) 1065, 2169, 2544, 4072, 4281, 4380, 4457
SmlI CTYRAG 6 cut(s) 65, 197, 519, 626, 2621, 3183
SmoI CTYRAG 6 cut(s) 65, 197, 519, 626, 2621, 3183
SpeI ACTAGT 3 cut(s) 212, 464, 2879
SphI GCATGC 3 cut(s) 771, 1757, 4742
SseBI AGGCCT 1 cut(s) 530
SsiI CCGC 6 cut(s) 2126, 2433, 2784, 3511, 4286, 4838
SspDI GGCGCC 1 cut(s) 1056
SspI AATATT 1 cut(s) 4615
SspMI CTAG 7 cut(s) 213, 465, 1581, 2312, 2847, 2880, 4802
SstI GAGCTC 1 cut(s) 3333
StuI AGGCCT 1 cut(s) 530
StyI CCWWGG 4 cut(s) 873, 1907, 3984, 4436
SwaI ATTTAAAT 1 cut(s) 1936
TaiI ACGT 3 cut(s) 3400, 4085, 4418
TaqI TCGA 4 cut(s) 50, 833, 950, 1379
TaqII GACCGA 2 cut(s) 3181, 3618
TatI WGTACW 8 cut(s) 75, 851, 1541, 1726, 1781, 2738, 3062, 3729
TscAI CASTG 6 cut(s) 37, 1953, 2647, 3055, 3584, 4939
TseFI GTSAC 6 cut(s) 705, 941, 2032, 2412, 3368, 4248
Tsp45I GTSAC 6 cut(s) 705, 941, 2032, 2412, 3368, 4248
TspGWI ACGGA 2 cut(s) 1510, 2473
TspRI CASTG 6 cut(s) 37, 1953, 2647, 3055, 3584, 4939
Vha464I CTTAAG 2 cut(s) 65, 2621
VpaK11BI GGWCC 5 cut(s) 1138, 1505, 2466, 2733, 3164
VspI ATTAAT 1 cut(s) 2610
XagI CCTNNNNNAGG 4 cut(s) 1582, 2845, 3207, 3590
XapI RAATTY 7 cut(s) 36, 1931, 2225, 2293, 2916, 3832, 4259
XbaI TCTAGA 1 cut(s) 1580
XceI RCATGY 6 cut(s) 608, 771, 949, 1757, 3904, 4742
XcmI CCANNNNNNNNNTGG 1 cut(s) 3152
XmnI GAANNNNTTC 1 cut(s) 621
XspI CTAG 7 cut(s) 213, 465, 1581, 2312, 2847, 2880, 4802
ZraI GACGTC 1 cut(s) 4416
ZrmI AGTACT 2 cut(s) 1543, 3731
Zsp2I ATGCAT 2 cut(s) 1767, 3603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.