MD08G1135600.v1.1

Enhancer of polycomb-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0010036
Physical Location & Seq
Reverse (-)
12863926 .. 12870541
6616 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1135600.v1.1.491

Sequence Viewer

Length: 5001 bp
ATGGAAAATAGAGTAGAGAACTCACATGGCACTGAAATTCCCGAAAAATCGAGATCTTTGGATCTGAAGAGTTTGTATAAATCGAGAAGTAGGAAAGATGTCGAAAACAAGAGCTTGAAGAGAAAAGTTAGTGCCGGGGATGGTGATGAGAACAGGGGCAAGACAAAGAAGAGTAAGAAAGAGGCTTCTCTTAGTAGTTTGAAGAATGTTAATACTAGTAGCAAAAAGAGTTTAGACAAAGTGTATCATAGCGGGTTGAATTCTGGTTCACATGATCCAGAGTCATGGAAATCTGGGTCGAGTGATAGATTGGATAGCAGTAGTGGGTTGAATGGTGTTTCGTCACTTAGTTTGAATAATAAGGTTATTCAAATCCCGAGGCGTAAACGGGGTTTTCTAGTGAGGAAGAAATTTGATGGTGGTCAGGCACCAAAGCTGCCAGATGAATCTGCTGGTAAAGCGGGTGTCATTGATCAGACTCATCAGATAGCTAAGTTAAATGGTGATGATTTAGGTACTCAATCTGAGTCCTTGAAAGTTAAACAAAAGAAGGGTCGGCATGATTTCAAAGAAAACATAAATAATGAGTTGAATTCAGCACCACATGCTAAGAAAGAAGACGTTCCTACAAGTCATTCGGCTGTAAGCAATGGTGATTCATCTTTGAAGAAGTCTCGGAGGAATCGTAGGAAGAGGAAGGAGTTGGCACCTGACAGTAAAAGCTCTGAAAAGGAAGCTGGGCCTTTGGTTGATAGTTCTATGAAAAAAGGCCATGATTTACAAGAAGATGATGAGGAGAATCTTGAACAGAATGCAGCAAGGATGCTTTCATCAAGGTTTGATCCCAGCTGCACTGGGTTTTCTTCAAACAACAAGGCTTCTGCAAATGGGTTGTCTTTTTTGTTGTCTTCTGGACAGGATTTTGATAGCCATAGGTCAAAGTCTATTTCTGGGTCAGAATCTCCTTCGGTTGACAATTCTGGTAGAGTATTGAGGCCGAGGAAACAACACAATGAAAAGGGACATTCGCGGAAAAGGCGCCATTTCTATGAAGTTTTCTTTGGGAACTTGGATGCTTACTGGGTTTTGAACCGGAGAATCAAAGTCTTCTGGCCTTTAGACCAAAGTTGGTACTATGGTCTTATTAATGACTATGACAAGGAAAAAAAACTTCATCATGTGAAATATGATGATCGTGACGAAGAATGGGTTGACCTTCAAAATGAGAGGTTCAAGCTCTTGTTACTCCCTAGTGAAGTTCCCGGTAGGACAGAGCGGAAAAAATCAAAGGTGCGGAATAGAAGTCCCGATGAGAGAAAAGGGGACAAGAAGTGCAGAAAAGAAAAGAAAAAGAGAGAATTGACTTCGGAGGATGATAGTGGCATAGGCAGCTATATAGACACTGAACCCATTATTTCATGGTTGGCACGATCTACGGGTCGGGTCAAGTCTCCTTCCTGTGCTGTGAAGAAGCAAAAAACATCTGGTCTATCTTTAAAGCCTGTGCCACCATTGTCTGATGAAGATGCCACTTTACATGAATCTTTGGGTGACAGCTCCTTTAAAAGGGATAAAAAAAATTCTCGCCATCCTGGCAGATCATCTGATGATGTGATGCAAGAAAAGCCTACCTCACAAGGCAGCACTGGCTCCAAAGATAGCAAAATGCCTATTGTTTATGTTCGAAGGCGGCTTCGGAAGAATGAGTCTGAATTGTCTCATACATCCAAGGATGACCATGACTCTGCGAGTAAACTTGGGTCACTATATGATTTCTTGGGAAGCTTGGATGCCAATGGACCATTGTGGTCTATTGATGATGCAGGACTGCTGAAATTAACTCCTCCACGGATAGAACCAGGAAGAGTTACTTTTGAGTTAGGCCTTCCAGTGCATTCAATTATTAATGATTCCTTTAGAGTAGAGTTTTGGTTATTTCGTGCCACAATGCTGCGTCGGTATGGTGCAGTGGTGATTTCATGGCCAAAGGTTTATTTGGAGATGCTTTTTGTTGACAATGTAGTTGGACTGAGATTTCTCTTGTTTGAAGGTTGCTTGAAGCAGGCTGTGGCCTTTGTTTTTCTTGTCCTGTCATTGTTTCATCAACCTAATGAGCAGGGGAAGTTTATTGATTTCCAGTTACCAGCAACTTCAATCAGGTTTAAATTCTCCAGTGTTCAGCATCTTGGGAAGCAACTTGTGTTTGCATTCTACAACTTTTCTGAAGTAAAGAATTCAAAGTGGAAGTACCTAGACTCTAAACTTACGAGCCATTGTTTGCTTACCAAGAAACTGCCCCCGTCTGAATGCACCTATGATAGTATTAAAGCACTTCAAAATGGAAGAAATCAGTCACCTTTTATGTCTCTTTGCGGGAATTCCTCCTTTGTCAAGGGTACACGGATTAGGCCCAGGCAGGGTATTAACTTCAAGGGGAGTTTCAGGGAATCTATATCTGTAAATAGTAGTGATTCTACTTCTCGTGATGATGAGCTCTGCAGAAAACTTCCTCCACTTGCCCTCTCTTTTGCTGCTGCACCTACTTTCTTCATTAGTTTGCATCTTAAGCTGCTTATGGAAAATTGTGTTGCTAATATCTGTTTTCGGGACCGTGATTCAGTGGAGCATGTCGAAAACTGTGACAATATGTTGGCAGTTGACTGGTCTGTTGTCGAGGACTTTATTAACGGAGGTTCGAAGATTACTCCTGAGAAAAATTTGAAGGCTGCACCAAGTAATGCTACTTCTGATGGATCCTGTGCCAAGCCAGATGCAGACAATGCTATTTCTCTATGCCATGGAGCTCGGACAAAGTCATCTCAGCATTTCCAAAATGGCAGTCTTGATGTTTCTGTATCCTCTGATGGTACTGGTGTCCTTGAAAAGACTGGAACAGATAAAGTTGTACAGTTGAAAGCGTTGCAAAGCCATCATCCAGAGTCAGATCAATGTTCTTTATCCCCAAGGCCTTTGGTTGGTAGAGACAAGTCCGACACTGATTCCCAATCTTTTCCGAATGGTCTCACTGTCGAAATTCCATCATTTGACCGATATGAGAAGCCTGTGGATAGGGAGGTGCAAAGTACTCAACAGCCTACAGAATTTTCTTGGAACATGAGTGGTAGCATTATTCCCAGCCCTAACCCCACTGCTCCCAGAAGTACAGGGCACCGAAATAGAAATAGTTCATCACTTGGACACCTCTCAAATAGTTGGACGGATGGAAAAGCTGACCTTTTCCACAATGGTTTTGGCAGTGGACCAAAGAAGCCCCGAACTCAGGTGTCCTATACATTGCCTTATGGAGGTTTTGACTTTAGTTCGAAGCAAAGAAACCTCCAGAAGGGGCTTTCTCATAAACGAATCAGGAGGGCTAATAATGAGAAGAGGTCATCAGATGCTTCCAGAGGTTCCCAAAGAAATTTGGAACTCTTATCATGTGAAACAAATGTGTTAGTCAATGGCAGTGACAGAGGGTGGAGGGAATGTGGGGCCCATGTTGTATTAGAGCTTTTTGATCATAATGAGTGGAAGCTTGCTGTAAAAATTTCAGGGACTACAAAGTACTCATACAAAGCACATCAATTTTTGCAGCCTGGGACAACAAACCGCTACACTCATGCTATGATGTGGAAAGGAGGAAAGGACTGGAATTGGGGTTTAGAGTTTCCAGATAGGAGTCAGTGGGCACTATTTAGGGAGATGCATGAAGAGTGTTACAATCGGAATATCCGCTCTGCTTCAGTAAAAAACATTCCTATTCCTGGTGTCCGCTTAATTGAAGAAAGTGATGATAATTCAATTGAAATATCATTTCTTCGCAGTTCTGCAAAGTATTTCCGGCAAATAGAAACAGATGTTGAAATGGCTTTGGACCCATCTCGGGTATTGTATGATATGGATAGTGATGATGAGCAGTGGATTTTGAAGTTTCAAAATTCTTCTGAAGTTCACAACAGTAGCTCAACAGAGATTGACGAGGAGATGTTTGAGAAAACAATGGACATGTTTGAAAAGGCTGCATATGATCAACAGTGTGATGAGTTCACATCTGAAGAAATAGAAGAGCTCATGGCTGGTGCTGGAGTTGGGCCCATGGATGTGATTTTATCCATTTATGAGCATTGGCTACAGAAAAGGCAGAGGAAGGGAATGCCTTTAATCCGACATCTTCAGCCACCATCATGGGAAAGGTATCAACAAGAAGTAAAAGAGTGGGAGCAAGCTATGATAAAAACCAATACCACCCTCCCCAATGGATGCTACGGGAAACCTGCATCAGTTGAGAAGCCACCAATGTTTGCTTTTTGTCTGAAACCACGGGGACTAGAAGTTCCCAACAAGGGATCAACACGGTCACAGAAGAAATTTTCGCTTTCTGGACATAATAGTGGCATGTTAGGAGATCACGATGGTTTCCATGCTATTGGAAGAAGATCAAATGGGTTTGCTTTCAGTGATGAAAGGCTGGCATATCCAGGCCATAACTATGACTCTTTAGACGACTCCCCCTTGTCTCAGACATCACCTGGGGTATTTTCACCAAGGGATGCAGCGAACATCTTGGTGAGCAATGATGGGTTTGAGAGAAATCATCTCCGCAGAATTCATAGAAGCAAGTCGAAGAAATTTGCGAGGACAGTATCTTATGTTGCCCCCCAGATGATGTCTTCATACAGTCCTAGAGTCGTAGGAAACAGAAATGAATTTCATCGATGGAATGCGGATATCCCTGATTGGTCAAGCCAGCGTTATTATCAACCAGAAGTGTCTCCGAGGCATGGCATGGGACTGTTGGATGATTCTGATCTCGATGAGTTCAGACTGCATGATGCATCTGGTGCCGCCCAACATGCACATAAAATGGCAAGGCTCAAGAGAGAGAGAGCTCGGAGATTGTTTTACAGAGCAGATCTAGCAATACACAGGGCCGTGGTTTCTCTCATGACCGCGGAAGCGATCAAAACTTCTTCTGAGGACTCCAGTGACGATGAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPL1

Protein Analysis

1667

Amino Acids

188.04

Weight (kDa)

9.11

Isoelectric Point (pI)

51.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPL1 PF10513 1243 - 1339 1.6e-11 Enhancer of polycomb-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 4282
AccB1I GGYRCC 5 cut(s) 427, 706, 1038, 3168, 4841
AccBSI CCGCTC 2 cut(s) 1276, 3735
AccII CGCG 2 cut(s) 1030, 4952
AclWI GGATC 6 cut(s) 69, 269, 836, 2748, 2761, 4354
AcoI YGGCCR 1 cut(s) 1982
AcuI CTGAAG 6 cut(s) 86, 2244, 3726, 3966, 4074, 4157
AcyI GRCGYC 1 cut(s) 1039
AfaI GTAC 9 cut(s) 517, 1133, 2249, 2398, 2869, 2907, 3085, 3163, 3566
AflII CTTAAG 1 cut(s) 2564
AflIII ACRYGT 1 cut(s) 4005
AhlI ACTAGT 1 cut(s) 215
AjnI CCWGG 7 cut(s) 1591, 1858, 2411, 3595, 3763, 4477, 4528
AjuI GAANNNNNNNTTGG 2 cut(s) 1044, 1076
AloI GAACNNNNNNTCC 2 cut(s) 4317, 4349
Alw21I GWGCWC 4 cut(s) 2496, 2806, 4071, 4891
Alw26I GTCTC 8 cut(s) 678, 1455, 1722, 2370, 2976, 3026, 4521, 4776
AlwI GGATC 6 cut(s) 69, 269, 836, 2748, 2761, 4354
Ama87I CYCGRG 2 cut(s) 376, 3882
ApaI GGGCCC 2 cut(s) 3496, 4095
ArsI GACNNNNNNTTYG 6 cut(s) 538, 570, 878, 910, 2824, 2856
AseI ATTAAT 2 cut(s) 1146, 1905
Asp700I GAANNNNTTC 2 cut(s) 621, 3184
AspLEI GCGC 1 cut(s) 1041
AsuC2I CCSGG 2 cut(s) 136, 1263
AsuHPI GGTGA 9 cut(s) 155, 515, 665, 1562, 1984, 2346, 4518, 4533, 4579
AsuII TTCGAA 3 cut(s) 1684, 2696, 3323
AvaI CYCGRG 2 cut(s) 376, 3882
AvaII GGWCC 4 cut(s) 1799, 2608, 3260, 3874
BaeGI GKGCMC 4 cut(s) 3171, 3496, 3691, 4095
BaeI ACNNNNGTAYC 4 cut(s) 1123, 1156, 2859, 2892
BalI TGGCCA 1 cut(s) 1984
BamHI GGATCC 1 cut(s) 2753
BanI GGYRCC 5 cut(s) 427, 706, 1038, 3168, 4841
BanII GRGCYC 6 cut(s) 2496, 2806, 3496, 4071, 4095, 4891
BarI GAAGNNNNNNTAC 2 cut(s) 2728, 2760
BauI CACGAG 1 cut(s) 2481
BbsI GAAGAC 4 cut(s) 624, 900, 1099, 4662
Bbv12I GWGCWC 4 cut(s) 2496, 2806, 4071, 4891
BceAI ACGGC 1 cut(s) 4916
BciT130I CCWGG 7 cut(s) 1593, 1860, 2413, 3597, 3765, 4479, 4530
BciVI GTATCC 1 cut(s) 2866
BclI TGATCA 3 cut(s) 472, 3517, 4027
BcnI CCSGG 2 cut(s) 136, 1263
BcoDI GTCTC 8 cut(s) 678, 1455, 1722, 2370, 2976, 3026, 4521, 4776
BcuI ACTAGT 1 cut(s) 215
BfaI CTAG 7 cut(s) 216, 398, 1251, 2252, 4328, 4683, 4916
BfmI CTRYAG 3 cut(s) 2497, 3096, 4130
BfoI RGCGCY 1 cut(s) 1042
BfrI CTTAAG 1 cut(s) 2564
BfuAI ACCTGC 1 cut(s) 4282
BfuI GTATCC 1 cut(s) 2866
BglI GCCNNNNNGGC 1 cut(s) 1593
BglII AGATCT 2 cut(s) 53, 4912
BmcAI AGTACT 2 cut(s) 3085, 3566
Bme1390I CCNGG 9 cut(s) 136, 1263, 1593, 1860, 2413, 3597, 3765, 4479, 4530
Bme18I GGWCC 4 cut(s) 1799, 2608, 3260, 3874
BmeT110I CYCGRG 2 cut(s) 376, 3882
BmrFI CCNGG 9 cut(s) 136, 1263, 1593, 1860, 2413, 3597, 3765, 4479, 4530
BmrI ACTGGG 2 cut(s) 864, 1090
BmuI ACTGGG 2 cut(s) 864, 1090
BpiI GAAGAC 4 cut(s) 624, 900, 1099, 4662
BpmI CTGGAG 4 cut(s) 2155, 3323, 4104, 4966
Bpu14I TTCGAA 3 cut(s) 1684, 2696, 3323
BpuEI CTTGAG 1 cut(s) 4859
BpuMI CCSGG 2 cut(s) 136, 1263
Bsa29I ATCGAT 1 cut(s) 4714
BsaBI GATNNNNATC 1 cut(s) 4806
BsaHI GRCGYC 1 cut(s) 1039
BsaI GGTCTC 1 cut(s) 3026
BsaWI WCCGGW 1 cut(s) 1092
BsaXI ACNNNNNCTCC 4 cut(s) 670, 700, 3472, 3502
Bse3DI GCAATG 3 cut(s) 655, 3293, 4579
Bse8I GATNNNNATC 1 cut(s) 4806
BseBI CCWGG 7 cut(s) 1593, 1860, 2413, 3597, 3765, 4479, 4530
BseCI ATCGAT 1 cut(s) 4714
BseJI GATNNNNATC 1 cut(s) 4806
BseMI GCAATG 3 cut(s) 655, 3293, 4579
BseMII CTCAG 7 cut(s) 516, 2021, 2700, 2834, 3293, 4532, 4965
BseRI GAGGAG 3 cut(s) 809, 1833, 3995
BseSI GKGCMC 4 cut(s) 3171, 3496, 3691, 4095
BseYI CCCAGC 3 cut(s) 737, 845, 3134
BsgI GTGCAG 5 cut(s) 835, 1354, 1986, 2520, 2712
Bsh1236I CGCG 2 cut(s) 1030, 4952
BshNI GGYRCC 5 cut(s) 427, 706, 1038, 3168, 4841
BshVI ATCGAT 1 cut(s) 4714
BsiHKAI GWGCWC 4 cut(s) 2496, 2806, 4071, 4891
BsiHKCI CYCGRG 2 cut(s) 376, 3882
BsiSI CCGG 4 cut(s) 135, 1093, 1263, 3841
BslFI GGGAC 8 cut(s) 1035, 1290, 1337, 2621, 3568, 3613, 4338, 4803
BsmAI GTCTC 8 cut(s) 678, 1455, 1722, 2370, 2976, 3026, 4521, 4776
BsmFI GGGAC 8 cut(s) 1035, 1290, 1337, 2621, 3568, 3613, 4338, 4803
BsmI GAATGC 6 cut(s) 817, 1894, 2207, 2312, 4158, 4726
Bso31I GGTCTC 1 cut(s) 3026
BsoBI CYCGRG 2 cut(s) 376, 3882
Bsp119I TTCGAA 3 cut(s) 1684, 2696, 3323
Bsp120I GGGCCC 2 cut(s) 3492, 4091
Bsp1286I GDGCHC 8 cut(s) 2496, 2806, 3171, 3496, 3691, 4071, 4095, 4891
Bsp1407I TGTACA 1 cut(s) 2905
Bsp19I CCATGG 2 cut(s) 2797, 4095
BspCNI CTCAG 7 cut(s) 517, 2022, 2701, 2833, 3292, 4531, 4966
BspDI ATCGAT 1 cut(s) 4714
BspFNI CGCG 2 cut(s) 1030, 4952
BspHI TCATGA 1 cut(s) 4944
BspMAI CTGCAG 1 cut(s) 2501
BspMI ACCTGC 1 cut(s) 4282
BspPI GGATC 6 cut(s) 69, 269, 836, 2748, 2761, 4354
BspQI GCTCTTC 1 cut(s) 4059
BspT104I TTCGAA 3 cut(s) 1684, 2696, 3323
BspT107I GGYRCC 5 cut(s) 427, 706, 1038, 3168, 4841
BspTI CTTAAG 1 cut(s) 2564
BspTNI GGTCTC 1 cut(s) 3026
BsrBI CCGCTC 2 cut(s) 1276, 3735
BsrDI GCAATG 3 cut(s) 655, 3293, 4579
BsrGI TGTACA 1 cut(s) 2905
BssNI GRCGYC 1 cut(s) 1039
BssSI CACGAG 1 cut(s) 2481
BssT1I CCWWGG 5 cut(s) 1728, 2797, 2963, 4095, 4544
Bst2BI CACGAG 1 cut(s) 2481
Bst2UI CCWGG 7 cut(s) 1593, 1860, 2413, 3597, 3765, 4479, 4530
Bst6I CTCTTC 9 cut(s) 62, 113, 164, 686, 1858, 3380, 3705, 4059, 4989
BstACI GRCGYC 1 cut(s) 1039
BstAFI CTTAAG 1 cut(s) 2564
BstAPI GCANNNNNTGC 3 cut(s) 605, 2780, 4841
BstAUI TGTACA 1 cut(s) 2905
BstBI TTCGAA 3 cut(s) 1684, 2696, 3323
BstC8I GCNNGC 5 cut(s) 2064, 3537, 4224, 4470, 4748
BstDSI CCRYGG 6 cut(s) 1847, 2797, 4095, 4319, 4932, 4950
BstENI CCTNNNNNAGG 3 cut(s) 1564, 3303, 3341
BstFNI CGCG 2 cut(s) 1030, 4952
BstH2I RGCGCY 1 cut(s) 1042
BstHHI GCGC 1 cut(s) 1041
BstMAI GTCTC 8 cut(s) 678, 1455, 1722, 2370, 2976, 3026, 4521, 4776
BstNI CCWGG 7 cut(s) 1593, 1860, 2413, 3597, 3765, 4479, 4530
BstNSI RCATGY 5 cut(s) 608, 2630, 4009, 4399, 4856
BstSCI CCNGG 9 cut(s) 134, 1261, 1591, 1858, 2411, 3595, 3763, 4477, 4528
BstSFI CTRYAG 3 cut(s) 2497, 3096, 4130
BstSLI GKGCMC 4 cut(s) 3171, 3496, 3691, 4095
BstUI CGCG 2 cut(s) 1030, 4952
BstV2I GAAGAC 4 cut(s) 624, 900, 1099, 4662
BstX2I RGATCY 4 cut(s) 53, 61, 2753, 4912
BstXI CCANNNNNNTGG 3 cut(s) 285, 4185, 4427
BstYI RGATCY 4 cut(s) 53, 61, 2753, 4912
Bsu15I ATCGAT 1 cut(s) 4714
BsuI GTATCC 1 cut(s) 2866
BsuTUI ATCGAT 1 cut(s) 4714
BtgI CCRYGG 6 cut(s) 1847, 2797, 4095, 4319, 4932, 4950
BtsI GCAGTG 5 cut(s) 1974, 3147, 3262, 3472, 3923
BveI ACCTGC 1 cut(s) 4282
Cac8I GCNNGC 5 cut(s) 2064, 3537, 4224, 4470, 4748
CciI TCATGA 1 cut(s) 4944
CfoI GCGC 1 cut(s) 1041
Cfr42I CCGCGG 1 cut(s) 4953
ClaI ATCGAT 1 cut(s) 4714
CseI GACGC 1 cut(s) 1943
Csp6I GTAC 9 cut(s) 516, 1132, 2248, 2397, 2868, 2906, 3084, 3162, 3565
CviQI GTAC 9 cut(s) 516, 1132, 2248, 2397, 2868, 2906, 3084, 3162, 3565
DinI GGCGCC 1 cut(s) 1040
DraI TTTAAA 3 cut(s) 1497, 1564, 2164
EaeI YGGCCR 1 cut(s) 1982
Eam1104I CTCTTC 9 cut(s) 62, 113, 164, 686, 1858, 3380, 3705, 4059, 4989
EarI CTCTTC 9 cut(s) 62, 113, 164, 686, 1858, 3380, 3705, 4059, 4989
Ecl136II GAGCTC 4 cut(s) 2494, 2804, 4069, 4889
Eco130I CCWWGG 5 cut(s) 1728, 2797, 2963, 4095, 4544
Eco147I AGGCCT 2 cut(s) 1884, 2968
Eco24I GRGCYC 6 cut(s) 2496, 2806, 3496, 4071, 4095, 4891
Eco31I GGTCTC 1 cut(s) 3026
Eco32I GATATC 1 cut(s) 4729
Eco47I GGWCC 4 cut(s) 1799, 2608, 3260, 3874
Eco53kI GAGCTC 4 cut(s) 2494, 2804, 4069, 4889
Eco57I CTGAAG 6 cut(s) 86, 2244, 3726, 3966, 4074, 4157
Eco88I CYCGRG 2 cut(s) 376, 3882
EcoICRI GAGCTC 4 cut(s) 2494, 2804, 4069, 4889
EcoNI CCTNNNNNAGG 3 cut(s) 1564, 3303, 3341
EcoO109I RGGNCCY 1 cut(s) 3492
EcoRI GAATTC 5 cut(s) 259, 592, 2233, 2377, 4605
EcoRII CCWGG 7 cut(s) 1591, 1858, 2411, 3595, 3763, 4477, 4528
EcoRV GATATC 1 cut(s) 4729
EcoT14I CCWWGG 5 cut(s) 1728, 2797, 2963, 4095, 4544
EcoT22I ATGCAT 2 cut(s) 3708, 4837
EcoT38I GRGCYC 6 cut(s) 2496, 2806, 3496, 4071, 4095, 4891
EgeI GGCGCC 1 cut(s) 1040
EheI GGCGCC 1 cut(s) 1040
ErhI CCWWGG 5 cut(s) 1728, 2797, 2963, 4095, 4544
FalI AAGNNNNNCTT 4 cut(s) 1855, 1887, 3219, 3251
FaqI GGGAC 8 cut(s) 1035, 1290, 1337, 2621, 3568, 3613, 4338, 4803
FauI CCCGC 3 cut(s) 245, 454, 2366
FauNDI CATATG 1 cut(s) 4024
FbaI TGATCA 3 cut(s) 472, 3517, 4027
FriOI GRGCYC 6 cut(s) 2496, 2806, 3496, 4071, 4095, 4891
FspBI CTAG 7 cut(s) 216, 398, 1251, 2252, 4328, 4683, 4916
GlaI GCGC 1 cut(s) 1040
GsaI CCCAGC 3 cut(s) 741, 849, 3138
GsuI CTGGAG 4 cut(s) 2155, 3323, 4104, 4966
HaeII RGCGCY 1 cut(s) 1042
HapII CCGG 4 cut(s) 135, 1093, 1263, 3841
HgaI GACGC 1 cut(s) 1943
HhaI GCGC 1 cut(s) 1041
Hin1I GRCGYC 1 cut(s) 1039
Hin6I GCGC 1 cut(s) 1039
HinP1I GCGC 1 cut(s) 1039
HincII GTYRAC 4 cut(s) 973, 1213, 2014, 2659
HindII GTYRAC 4 cut(s) 973, 1213, 2014, 2659
HindIII AAGCTT 2 cut(s) 1783, 3533
HpaII CCGG 4 cut(s) 135, 1093, 1263, 3841
HphI GGTGA 9 cut(s) 155, 515, 665, 1562, 1984, 2346, 4518, 4533, 4579
Hpy99I CGWCG 1 cut(s) 1959
HpyCH4IV ACGT 1 cut(s) 621
HpySE526I ACGT 1 cut(s) 621
Hsp92I GRCGYC 1 cut(s) 1039
HspAI GCGC 1 cut(s) 1039
KasI GGCGCC 1 cut(s) 1038
Ksp22I TGATCA 3 cut(s) 472, 3517, 4027
KspI CCGCGG 1 cut(s) 4953
LguI GCTCTTC 1 cut(s) 4059
LmnI GCTCC 6 cut(s) 1562, 1655, 2623, 2801, 3157, 4219
MaeI CTAG 7 cut(s) 216, 398, 1251, 2252, 4328, 4683, 4916
MaeII ACGT 1 cut(s) 621
MbiI CCGCTC 2 cut(s) 1276, 3735
MfeI CAATTG 1 cut(s) 3801
MflI RGATCY 4 cut(s) 53, 61, 2753, 4912
MhlI GDGCHC 8 cut(s) 2496, 2806, 3171, 3496, 3691, 4071, 4095, 4891
MlsI TGGCCA 1 cut(s) 1984
MluNI TGGCCA 1 cut(s) 1984
Mly113I GGCGCC 1 cut(s) 1039
MmeI TCCRAC 5 cut(s) 2005, 3015, 3194, 4189, 4776
Mox20I TGGCCA 1 cut(s) 1984
Mph1103I ATGCAT 2 cut(s) 3708, 4837
MroXI GAANNNNTTC 2 cut(s) 621, 3184
MscI TGGCCA 1 cut(s) 1984
MslI CAYNNNNRTG 6 cut(s) 1047, 4100, 4183, 4389, 4488, 4565
Msp20I TGGCCA 1 cut(s) 1984
MspA1I CMGCKG 2 cut(s) 849, 4952
MspCI CTTAAG 1 cut(s) 2564
MspI CCGG 4 cut(s) 135, 1093, 1263, 3841
MspR9I CCNGG 9 cut(s) 136, 1263, 1593, 1860, 2413, 3597, 3765, 4479, 4530
MunI CAATTG 1 cut(s) 3801
Mva1269I GAATGC 6 cut(s) 817, 1894, 2207, 2312, 4158, 4726
MvaI CCWGG 7 cut(s) 1593, 1860, 2413, 3597, 3765, 4479, 4530
MvnI CGCG 2 cut(s) 1030, 4952
NarI GGCGCC 1 cut(s) 1039
NciI CCSGG 2 cut(s) 136, 1263
NcoI CCATGG 2 cut(s) 2797, 4095
NdeI CATATG 1 cut(s) 4024
NmeAIII GCCGAG 1 cut(s) 1023
NmuCI GTSAC 9 cut(s) 342, 1196, 1550, 1761, 2352, 2639, 3467, 4356, 4985
NsiI ATGCAT 2 cut(s) 3708, 4837
NspI RCATGY 5 cut(s) 608, 2630, 4009, 4399, 4856
NspV TTCGAA 3 cut(s) 1684, 2696, 3323
PagI TCATGA 1 cut(s) 4944
PceI AGGCCT 2 cut(s) 1884, 2968
PciI ACATGT 1 cut(s) 4005
PciSI GCTCTTC 1 cut(s) 4059
PcsI WCGNNNNNNNCGW 1 cut(s) 682
PctI GAATGC 6 cut(s) 817, 1894, 2207, 2312, 4158, 4726
PdmI GAANNNNTTC 2 cut(s) 621, 3184
PflFI GACNNNGTC 1 cut(s) 2812
PluTI GGCGCC 1 cut(s) 1042
PscI ACATGT 1 cut(s) 4005
PshBI ATTAAT 2 cut(s) 1146, 1905
Psp124BI GAGCTC 4 cut(s) 2496, 2806, 4071, 4891
Psp6I CCWGG 7 cut(s) 1591, 1858, 2411, 3595, 3763, 4477, 4528
PspFI CCCAGC 3 cut(s) 737, 845, 3134
PspGI CCWGG 7 cut(s) 1591, 1858, 2411, 3595, 3763, 4477, 4528
PspOMI GGGCCC 2 cut(s) 3492, 4091
PstI CTGCAG 1 cut(s) 2501
PsuI RGATCY 4 cut(s) 53, 61, 2753, 4912
PsyI GACNNNGTC 1 cut(s) 2812
PvuII CAGCTG 1 cut(s) 849
RsaI GTAC 9 cut(s) 517, 1133, 2249, 2398, 2869, 2907, 3085, 3163, 3566
RsaNI GTAC 9 cut(s) 516, 1132, 2248, 2397, 2868, 2906, 3084, 3162, 3565
RseI CAYNNNNRTG 6 cut(s) 1047, 4100, 4183, 4389, 4488, 4565
SacI GAGCTC 4 cut(s) 2496, 2806, 4071, 4891
SacII CCGCGG 1 cut(s) 4953
SapI GCTCTTC 1 cut(s) 4059
ScaI AGTACT 2 cut(s) 3085, 3566
ScrFI CCNGG 9 cut(s) 136, 1263, 1593, 1860, 2413, 3597, 3765, 4479, 4530
SduI GDGCHC 8 cut(s) 2496, 2806, 3171, 3496, 3691, 4071, 4095, 4891
SfcI CTRYAG 3 cut(s) 2497, 3096, 4130
SfoI GGCGCC 1 cut(s) 1040
Sfr303I CCGCGG 1 cut(s) 4953
SfuI TTCGAA 3 cut(s) 1684, 2696, 3323
SgrBI CCGCGG 1 cut(s) 4953
SinI GGWCC 4 cut(s) 1799, 2608, 3260, 3874
SmiMI CAYNNNNRTG 6 cut(s) 1047, 4100, 4183, 4389, 4488, 4565
SmlI CTYRAG 2 cut(s) 2564, 4874
SmoI CTYRAG 2 cut(s) 2564, 4874
SpeI ACTAGT 1 cut(s) 215
SseBI AGGCCT 2 cut(s) 1884, 2968
SspDI GGCGCC 1 cut(s) 1038
SspMI CTAG 7 cut(s) 216, 398, 1251, 2252, 4328, 4683, 4916
SstI GAGCTC 4 cut(s) 2496, 2806, 4071, 4891
StuI AGGCCT 2 cut(s) 1884, 2968
StyD4I CCNGG 9 cut(s) 134, 1261, 1591, 1858, 2411, 3595, 3763, 4477, 4528
StyI CCWWGG 5 cut(s) 1728, 2797, 2963, 4095, 4544
TaiI ACGT 1 cut(s) 624
TaqII GACCGA 1 cut(s) 3063
TatI WGTACW 4 cut(s) 2905, 3083, 3161, 3564
TauI GCSGC 2 cut(s) 1693, 4847
TseFI GTSAC 9 cut(s) 342, 1196, 1550, 1761, 2352, 2639, 3467, 4356, 4985
Tsp45I GTSAC 9 cut(s) 342, 1196, 1550, 1761, 2352, 2639, 3467, 4356, 4985
TspGWI ACGGA 4 cut(s) 1864, 2416, 2703, 3233
Tth111I GACNNNGTC 1 cut(s) 2812
Vha464I CTTAAG 1 cut(s) 2564
VpaK11BI GGWCC 4 cut(s) 1799, 2608, 3260, 3874
VspI ATTAAT 2 cut(s) 1146, 1905
XagI CCTNNNNNAGG 3 cut(s) 1564, 3303, 3341
XceI RCATGY 5 cut(s) 608, 2630, 4009, 4399, 4856
XcmI CCANNNNNNNNNTGG 1 cut(s) 3248
XmnI GAANNNNTTC 2 cut(s) 621, 3184
XspI CTAG 7 cut(s) 216, 398, 1251, 2252, 4328, 4683, 4916
ZrmI AGTACT 2 cut(s) 3085, 3566
Zsp2I ATGCAT 2 cut(s) 3708, 4837
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.