Rroxscaffold_7G00156580

Enhancer of polycomb-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0010036
Physical Location & Seq
Forward (+)
396102 .. 401832
5731 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00156580.1

Sequence Viewer

Length: 4821 bp
ATGGAAAATAGAATAGAGGTTTCACATGGCACTGAAATTCCCAGGAAATCGAGATCTTTGGATCTTAAGAGTTTGTACAAGTCCAGGAGTAGAAAAGAGGCTGAAAATCAGAGCCTGAAGAGAAAAGGGAGTGAGGACGACGACGGTGGGGACAAGAAGAAGAGGAGTAGGAAAGAGGTGTCTCTCAGTAGTCTCAAGAATGTTAATACTAGTAGTAGCAAGAAGAGTATAGATAAAGTTTATCATAGAGGGTTGGATTCTGGTTCACATGATCCGGACGCATTCAAATCGGGGTCAATTCAAAAATTGGATAGCAGTAGTGGGTTAAATGGCGTTTCAAAGCTTAGTTTGGATAATAGTGGCATTCAAATCCCGAGGCGTAAGAGGGGATTCGTGGGGCGAAAAAAATGTGAGGGTGCTCAGCTGTTGAAGCTGTCAGATGAGTCTGCCGGTAGAGCAACTAGTGTTGATCAGAATCATCAGATAGCTAACTTAAGTGGTGATGATTTGGGTACTCAAGCTGAGGCCTGGAAGGCCAAAAGAAACAAAGGTTTGGATGAATGCAAAGAAAATATAAATAGTGAGTTGAATGCAACTTCACATGCAAAGAAAGAAAATGTTCTTGAGAGGCAGTCAGTTGCAAGCAATGGTAATTCATCTTTGAAAAAGTCCCGGAGGAATCGTCGGAAAAGGAAGGACTTGTCACCTGATAGTAAAACTGATGCAAAGGAAGCTGAGCCTTTAGTCAATAGTTCTACTAAAGCATGCCAGGCTTCACAAGAAGACGAGGAGGAGAATCTTGAAGAGAATGCAGCGAGGATGCTTTCATCGAGGTTTGATCCAAGCTGCACTGGGTTTTCATTAAACACCAAGGCTTCTGCGATGCAATCTTCAAACGGGTTGTCTAATCAGTCATCTCCTGGTCAGGATTTTGATAGTCACATGTCGAAGTCATTTTCTGGATCAGAATCTCAATCTGTGGATAATGCTGGTAGAGTGCTGAGGCCCAGGGCCAGAAAGCAGCACAAAGAAAAGAAAGGTACGAGGAAGAGGCGCCACTTCTATGAAATCTTCTTCGGGGACTTGGATGCTTGTTGGGTATTGAACCGTAGGATCAAGGTCTTCTGGCCTTTGGACCAAAGTTGGTATTATGGCCTTGTGAATGATTATGACAAGGAGAAAAAACTTCACCATATTAGATACGATGACCGTGAAGAAGAATGGGTTGATCTTCAACATGAGCGGTTCAAGCTCTTGCTACTTCCTACTGAAGTTCCTGGTAAGGCAAAGCAGAAAAAATCAATCATGCAAATTACAAGTTCTGAAGAGAGAGAAGGAAACTTGAAACCCAGAAAAGAGAAGAAAAAGAGAGACTCGATGTCGGACGACGATAGCTGCATAGGCAGCTGTATGGATTCTGAACCTATTATTTCATGGTTGTCGCGATCTACCCGTCGGATCAAGTCTCCTTCTCATGCTGTGAAGAAGCAAAAAACAACTGGTCTATCTCCAAAGTCCGTGCCAACTTTGTCTGACAGTGCTGGCACTTTACATGGGTGTTTGAGTGATGTTTCCTCTAGAAGGGATACAAATAAATTATCTTGTAATTCCGGCAGATCATCTGATCCTGTGAGGGAAGAGAAGCCTGCTCCAGAAGGCAACTCCGAAGATAGCAAAATGCCTATTGTTTATTATCGGAAGCGATTGCGCAAGACTGGCTCTGTACTGTCTCAAATATACAAGGATGAGCATGCTTCCATGCATGGACATCATTGTTGTACTTCTGTTACTCCTGTTGAGGAGGGTTGGGACTTGGAAGAGCCAGATGATTTTGTTGTTATATTGGATCGCAGTTGGCCATTGTGGTATAGTGATGATGCAGGGTTGCTAAAGTTAACTCTTCCTTGGATAGAATCAGGAAAAGTCAAATTTAAATGCCTTCCACTGCATTCACTAATGAATGATTCTCTTGGAGTAGAGTTGTTACGTTTCTTTCATGCTGCAATGCTGCTTCGGCATGGTGTAGTGGTGACCACATGGCCAAAGGTTCATTTGGAGATGCTCTTTGTTGATAATGTAGTTGGACTGAGGTTTCTTTTGTTTGAAGGTTGCTTGAAGCAGGCAGTGGCCTTTGTTTCTCTTATCCTGACATTATTTCATCACCCTAATGAACAGGGGAAGCTTACTGATTTCCAGTTACCAGCAACTTCAATCAGGTTTAAATTCTCCTGTGTTCAGCATCTTGGAAAGCAACTTGTGTCTGCTTTCTACAACTTCTGTCAAGTTAAAAATTCAACATGGATGTACCTAGACAATAAACTCAGGAGGCATTGTTTACTCGCCAAAAAACTGCCTCTGTCTGAATGCACCTATGATAATATCCAGGCTCTTCAAAATGGAATAAATCAGTCACCTTCTATGTCTCTTTGTGGGCAACCCTCTGTCAAGATAATTATGCTTGCTATTTATTCCTCTTCAATGCTGTATTATGAAAGTATTCACTTCTGTGATTTGTCTCCAAGCAGGAAAGCTTCCCCACTTGCTCTATCTTTTACAGCTGCACCTACTTTCTTCATTAATTTGCATCTTAAGTTGCTTATGGAACACAGTGTTGCTAATATCTGCTTTCAAGACCATGATTCAGAAATTACTGCCGAGAACAATTTGAAGTCTCCAGCAAATGTGGCTACCTCTGGTGGGTCCTGTACTAAAGTGGTAACAGAAACATCTCTTTCTATTTGCAGTCAGCGCGGTCGGTTAAAGTCATCTCAGCTTTACCAAAATTGTGTTGTTAATGTTGCTGGAGCCTCTTCTAGGACTTTTGCTGGCAGAGATAAATCCGAAACTAGTTCCCGTTCTATTGTGAATGGGCTTACTGTTGAAATTCCATCTTTTGACCATTTTGAGAAGCCTGTGGAGAGGGAGTTACAAAGTGCTCAACAGCCTACAGTTTTTTCATTGAATATGAATGGTAGCATTATCCCCAGTCCCAGCCCCACTGCTCCCAGAAGTACAGGGCAGCGAAATAGAAATAGTATGTCATCATTTGGAAACCTCGCACATCGTTGGTCAGATGGAAAGGCTGACATTTTCCATAATGGTTTTGGGAATGGACCGAAGAAGCCCCGAACTCAAGTCTCATACACATTGCCTTGTGGAGGTTTTGATGTCAGCTCTAAGCAAAGAAATGTTCACAAAGGGCTTCCTAATAAGCGAATTAGAAGGGCCAGCGAGAAGAGGTCATCAGACATTTCCAGAGGTTCCGAAAGGAACTTGGAGCTCTTATCCTGTGAGGCAAATGTGTTAATTTCTGCAAGTGACAGAGGGTGGAGAGAATGTGGGGCACGTGTAGTATTAGAACTCTTTGATAATAGTGAGTGGAAGCTTGCTGTAAAACTTTCAGGGTCTACGAAGTATTCATACAAAGCACATCAATTTTTGCAGCCTGGGTCAACAAACCGCTATACTCATGTTATGATGTGGAAAGGAGGAAAGGATTGGATCTTGGAGTTTCCAGATAGGAGCCAGTGGGCCCTTTTCAAGGAGATGCATGAAGAGTGTTACAATCGGAACCTTCGGTCGGCGTCAGTTAAAAACATTCCTATTCCTGGTGTTCACTTAGTAGAAGATATTGATGATAATGGAACAGAAATAGCATTTCTTCGCAGTTCTACCAAGTACTTTCAGCAGATGAAAACAGATGTTGAGATGGCTTTAGATCCATCTCGTCTCTTGTATGACATGGATAGTGATGATGAGCGATGGATTTTGAAGTTTCAAAATTCTTCAGAAGTTGAAAAGAGTAGCTCAATGGAGATTGGCGAGGAGATGTTTGAGAAGACAATGGACATGTTTGAGAAGGCTGCATATGTTCGGCAATGTGATCAGTTCACATCTGAAGAAATAGAAGAGTTCATGACTGGTATTGGGCCCATGGATTTGATTAAAACCATTTATGAGCATTGGCGTCAGAAACGGCTGAGAAAGGGAATGCCTTTAATCCGACATCTCCAGCCACCGTCATGGGAGACGTATCAAAAACAAGTGAGGGAGTGGGAGCAAGCTATGACCAAGATGAATAACACACTCGCAAATGGTGGCCGTGAGAAAGCTGCAGCAGTTGAGAAGCCACCGATGTATGCTTTTTGTCTGAAACCACGGGGCCTGGAAGTTCCCAATAAGGGATCAAAGCAACGGTCACAGAAGAAATTTTCTATTTCTGCACATACCAATGCGGTGTTAGGAGATCAGGATGGTTTCCATCCTATAGATGACTCTCCATTGTCTCAGACGTCGCCCAGGGTTTTTTCACCAAGGGATGCAGCAAACATCTTTATGAGCAATGATGGTTTTGAGAGGAATCATCTTCACAGAATTGACAGAAGCAAGTCAAAGAAATATCGAACAATAGCGTCCTCTGTTGACCCCCAGATGGTGTCTCCATATAATCATAGAGTGGTACGAAACAGAAATGGAGTTCATAGAGGGAATATTGGTTTCCCGGAGTGGTCAAGCCAGTCATATTATCAACCAGATGTTGCTCAGAGGCTTGTAAATGCGCATGGCTTTGATCATGATGAGTTTAGGCTTCGTGAGGCATCCGGTGCTGCTCAGCATGCACATAACTTAGCCAAGCGTAAGAGAGAAAATGCTCGCAGATTGTTTTACAGAGCAGATCTAGCAATGCACAAGGCTGTGGTTGCTCTCATGACTGCGGAAGCAATCAAAGCTTCTTCAGAGGACTACGACTATGACTACGACTCTGAGTCCGACTCCTTCTCCATCTCCGACTCTGACACCAATTCAGACAGTGAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPL1

Protein Analysis

1606

Amino Acids

181.61

Weight (kDa)

9.07

Isoelectric Point (pI)

58.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPL1 PF10513 1192 - 1287 4.7e-08 Enhancer of polycomb-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 4295
Acc16I TGCGCA 2 cut(s) 1709, 4560
AccB1I GGYRCC 1 cut(s) 1053
AccBSI CCGCTC 1 cut(s) 1243
AccI GTMKAC 1 cut(s) 3407
AccII CGCG 2 cut(s) 1444, 2751
AccIII TCCGGA 1 cut(s) 274
AciI CCGC 5 cut(s) 1243, 2751, 3460, 4235, 4715
AcoI YGGCCR 3 cut(s) 1856, 2039, 4099
AcsI RAATTY 7 cut(s) 36, 1928, 2222, 2290, 2883, 3781, 4208
AcuI CTGAAG 6 cut(s) 137, 1290, 1344, 3771, 3918, 4719
AcvI CACGTG 1 cut(s) 3347
AcyI GRCGYC 4 cut(s) 1054, 3584, 3967, 4292
AfiI CCNNNNNNNGG 9 cut(s) 1581, 2697, 2814, 3158, 3541, 3580, 3608, 4181, 4432
AflII CTTAAG 3 cut(s) 65, 493, 2588
AflIII ACRYGT 3 cut(s) 942, 3346, 3849
AhdI GACNNNNNGTC 2 cut(s) 1378, 4765
AhlI ACTAGT 3 cut(s) 209, 461, 2846
AleI CACNNNNGTG 1 cut(s) 2505
Alw21I GWGCWC 3 cut(s) 421, 2938, 3282
AlwNI CAGNNNCTG 1 cut(s) 115
Ama87I CYCGRG 1 cut(s) 373
Aor13HI TCCGGA 1 cut(s) 274
ApaI GGGCCC 2 cut(s) 3535, 3933
ApoI RAATTY 7 cut(s) 36, 1928, 2222, 2290, 2883, 3781, 4208
ArsI GACNNNNNNTTYG 4 cut(s) 887, 919, 4181, 4213
AseI ATTAAT 1 cut(s) 2577
Asp700I GAANNNNTTC 2 cut(s) 618, 2496
AspLEI GCGC 4 cut(s) 1056, 1710, 2751, 4561
AsuC2I CCSGG 2 cut(s) 673, 4502
AsuHPI GGTGA 7 cut(s) 512, 696, 1181, 2041, 2153, 2403, 4302
AvaI CYCGRG 1 cut(s) 373
AvaII GGWCC 3 cut(s) 1135, 2700, 3113
BaeGI GKGCMC 3 cut(s) 3346, 3535, 3933
BalI TGGCCA 2 cut(s) 1858, 2041
BanI GGYRCC 1 cut(s) 1053
BanII GRGCYC 3 cut(s) 3282, 3535, 3933
BarI GAAGNNNNNNTAC 2 cut(s) 3404, 3436
BbrPI CACGTG 1 cut(s) 3347
BbsI GAAGAC 3 cut(s) 789, 1114, 3845
Bbv12I GWGCWC 3 cut(s) 421, 2938, 3282
BbvCI CCTCAGC 2 cut(s) 522, 1001
BceAI ACGGC 2 cut(s) 3992, 4086
BcgI CGANNNNNNTGC 2 cut(s) 270, 304
BciVI GTATCC 1 cut(s) 1579
BclI TGATCA 3 cut(s) 469, 3883, 4570
BcnI CCSGG 2 cut(s) 673, 4502
BcuI ACTAGT 3 cut(s) 209, 461, 2846
BfaI CTAG 7 cut(s) 210, 462, 1578, 2309, 2814, 2847, 4679
BfmI CTRYAG 3 cut(s) 2946, 4113, 4266
BfoI RGCGCY 1 cut(s) 1057
BfrI CTTAAG 3 cut(s) 65, 493, 2588
BfuI GTATCC 1 cut(s) 1579
BglI GCCNNNNNGGC 1 cut(s) 533
BglII AGATCT 2 cut(s) 53, 4675
BlpI GCTNAGC 3 cut(s) 420, 735, 4611
BmcAI AGTACT 1 cut(s) 3680
Bme18I GGWCC 3 cut(s) 1135, 2700, 3113
BmeRI GACNNNNNGTC 2 cut(s) 1378, 4765
BmeT110I CYCGRG 1 cut(s) 373
BmiI GGNNCC 9 cut(s) 1055, 2701, 2806, 3262, 3524, 3533, 3572, 3931, 4163
BmrI ACTGGG 2 cut(s) 861, 2979
BmuI ACTGGG 2 cut(s) 861, 2979
BpiI GAAGAC 3 cut(s) 789, 1114, 3845
BplI GAGNNNNNCTC 2 cut(s) 4757, 4789
BpmI CTGGAG 4 cut(s) 1635, 2658, 2823, 3995
Bpu10I CCTNAGC 2 cut(s) 522, 1001
Bpu1102I GCTNAGC 3 cut(s) 420, 735, 4611
BpuEI CTTGAG 4 cut(s) 179, 501, 644, 3117
BpuMI CCSGG 2 cut(s) 673, 4502
BsaAI YACGTR 1 cut(s) 3347
BsaBI GATNNNNATC 2 cut(s) 474, 967
BsaHI GRCGYC 4 cut(s) 1054, 3584, 3967, 4292
BsaWI WCCGGW 2 cut(s) 274, 4601
Bsc4I CCNNNNNNNGG 9 cut(s) 1581, 2697, 2814, 3158, 3541, 3580, 3608, 4181, 4432
Bse118I RCCGGY 1 cut(s) 449
Bse1I ACTGG 8 cut(s) 856, 1504, 1720, 2194, 2985, 3526, 3925, 4516
Bse3DI GCAATG 6 cut(s) 652, 2010, 3146, 3884, 4348, 4689
Bse8I GATNNNNATC 2 cut(s) 474, 967
BseAI TCCGGA 1 cut(s) 274
BseGI GGATG 9 cut(s) 562, 825, 1093, 1750, 2307, 4258, 4261, 4324, 4598
BseJI GATNNNNATC 2 cut(s) 474, 967
BseLI CCNNNNNNNGG 9 cut(s) 1581, 2697, 2814, 3158, 3541, 3580, 3608, 4181, 4432
BseMI GCAATG 6 cut(s) 652, 2010, 3146, 3884, 4348, 4689
BseNI ACTGG 8 cut(s) 856, 1504, 1720, 2194, 2985, 3526, 3925, 4516
BseRI GAGGAG 5 cut(s) 178, 803, 806, 1814, 3839
BseSI GKGCMC 3 cut(s) 3346, 3535, 3933
BseYI CCCAGC 1 cut(s) 2990
BsgI GTGCAG 3 cut(s) 832, 2544, 4206
Bsh1236I CGCG 2 cut(s) 1444, 2751
Bsh1285I CGRYCG 2 cut(s) 2755, 3581
BshNI GGYRCC 1 cut(s) 1053
BsiEI CGRYCG 2 cut(s) 2755, 3581
BsiHKAI GWGCWC 3 cut(s) 421, 2938, 3282
BsiHKCI CYCGRG 1 cut(s) 373
BsiSI CCGG 6 cut(s) 275, 450, 673, 1611, 4502, 4602
BslFI GGGAC 5 cut(s) 164, 655, 1094, 1823, 2973
BslI CCNNNNNNNGG 9 cut(s) 1581, 2697, 2814, 3158, 3541, 3580, 3608, 4181, 4432
BsmBI CGTCTC 2 cut(s) 3734, 4022
BsmFI GGGAC 5 cut(s) 164, 655, 1094, 1823, 2973
BsmI GAATGC 8 cut(s) 281, 363, 566, 595, 814, 1948, 2369, 3996
BsoBI CYCGRG 1 cut(s) 373
Bsp120I GGGCCC 2 cut(s) 3531, 3929
Bsp1286I GDGCHC 6 cut(s) 421, 2938, 3282, 3346, 3535, 3933
Bsp13I TCCGGA 1 cut(s) 274
Bsp1407I TGTACA 1 cut(s) 75
Bsp1720I GCTNAGC 3 cut(s) 420, 735, 4611
Bsp19I CCATGG 1 cut(s) 3933
Bsp68I TCGCGA 1 cut(s) 1444
BspACI CCGC 5 cut(s) 1243, 2751, 3460, 4235, 4715
BspEI TCCGGA 1 cut(s) 274
BspFNI CGCG 2 cut(s) 1444, 2751
BspHI TCATGA 3 cut(s) 3915, 4573, 4707
BspLI GGNNCC 9 cut(s) 1055, 2701, 2806, 3262, 3524, 3533, 3572, 3931, 4163
BspMAI CTGCAG 1 cut(s) 4117
BspQI GCTCTTC 2 cut(s) 1812, 2394
BspT107I GGYRCC 1 cut(s) 1053
BspTI CTTAAG 3 cut(s) 65, 493, 2588
BsrBI CCGCTC 1 cut(s) 1243
BsrDI GCAATG 6 cut(s) 652, 2010, 3146, 3884, 4348, 4689
BsrFI RCCGGY 1 cut(s) 449
BsrGI TGTACA 1 cut(s) 75
BsrI ACTGG 8 cut(s) 856, 1504, 1720, 2194, 2985, 3526, 3925, 4516
BssAI RCCGGY 1 cut(s) 449
BssNI GRCGYC 4 cut(s) 1054, 3584, 3967, 4292
BssT1I CCWWGG 4 cut(s) 870, 1904, 3933, 4313
BstACI GRCGYC 4 cut(s) 1054, 3584, 3967, 4292
BstAFI CTTAAG 3 cut(s) 65, 493, 2588
BstAPI GCANNNNNTGC 1 cut(s) 4604
BstAUI TGTACA 1 cut(s) 75
BstBAI YACGTR 1 cut(s) 3347
BstDSI CCRYGG 2 cut(s) 3933, 4157
BstEII GGTNACC 1 cut(s) 2029
BstENI CCTNNNNNAGG 4 cut(s) 1579, 2812, 3156, 3539
BstF5I GGATG 9 cut(s) 562, 825, 1093, 1750, 2307, 4258, 4261, 4324, 4598
BstFNI CGCG 2 cut(s) 1444, 2751
BstH2I RGCGCY 1 cut(s) 1057
BstHHI GCGC 4 cut(s) 1056, 1710, 2751, 4561
BstMCI CGRYCG 2 cut(s) 2755, 3581
BstNSI RCATGY 6 cut(s) 605, 768, 946, 1754, 3853, 4619
BstPI GGTNACC 1 cut(s) 2029
BstSFI CTRYAG 3 cut(s) 2946, 4113, 4266
BstSLI GKGCMC 3 cut(s) 3346, 3535, 3933
BstUI CGCG 2 cut(s) 1444, 2751
BstV2I GAAGAC 3 cut(s) 789, 1114, 3845
BstX2I RGATCY 5 cut(s) 53, 61, 3501, 3718, 4675
BstXI CCANNNNNNTGG 1 cut(s) 4023
BstYI RGATCY 5 cut(s) 53, 61, 3501, 3718, 4675
BsuI GTATCC 1 cut(s) 1579
BtgI CCRYGG 2 cut(s) 3933, 4157
BtgZI GCGATG 2 cut(s) 896, 3775
BtsCI GGATG 9 cut(s) 562, 825, 1093, 1750, 2307, 4258, 4261, 4324, 4598
BtsI GCAGTG 3 cut(s) 1943, 2130, 2997
BtsIMutI CAGTG 9 cut(s) 30, 849, 1543, 1943, 2130, 2614, 2997, 3533, 4816
BtuMI TCGCGA 1 cut(s) 1444
CaiI CAGNNNCTG 1 cut(s) 115
CciI TCATGA 3 cut(s) 3915, 4573, 4707
CfoI GCGC 4 cut(s) 1056, 1710, 2751, 4561
Cfr10I RCCGGY 1 cut(s) 449
CseI GACGC 4 cut(s) 287, 3573, 3956, 4401
DinI GGCGCC 1 cut(s) 1055
DraI TTTAAA 2 cut(s) 1933, 2221
DriI GACNNNNNGTC 2 cut(s) 1378, 4765
EaeI YGGCCR 3 cut(s) 1856, 2039, 4099
Eam1105I GACNNNNNGTC 2 cut(s) 1378, 4765
Ecl136II GAGCTC 1 cut(s) 3280
Eco130I CCWWGG 4 cut(s) 870, 1904, 3933, 4313
Eco147I AGGCCT 1 cut(s) 527
Eco24I GRGCYC 3 cut(s) 3282, 3535, 3933
Eco47I GGWCC 3 cut(s) 1135, 2700, 3113
Eco53kI GAGCTC 1 cut(s) 3280
Eco57I CTGAAG 6 cut(s) 137, 1290, 1344, 3771, 3918, 4719
Eco72I CACGTG 1 cut(s) 3347
Eco88I CYCGRG 1 cut(s) 373
Eco91I GGTNACC 1 cut(s) 2029
EcoICRI GAGCTC 1 cut(s) 3280
EcoNI CCTNNNNNAGG 4 cut(s) 1579, 2812, 3156, 3539
EcoO109I RGGNCCY 3 cut(s) 2700, 3532, 4162
EcoO65I GGTNACC 1 cut(s) 2029
EcoT14I CCWWGG 4 cut(s) 870, 1904, 3933, 4313
EcoT22I ATGCAT 2 cut(s) 1764, 3552
EcoT38I GRGCYC 3 cut(s) 3282, 3535, 3933
EgeI GGCGCC 1 cut(s) 1055
EheI GGCGCC 1 cut(s) 1055
ErhI CCWWGG 4 cut(s) 870, 1904, 3933, 4313
Esp3I CGTCTC 2 cut(s) 3734, 4022
FaqI GGGAC 5 cut(s) 164, 655, 1094, 1823, 2973
FauNDI CATATG 1 cut(s) 3868
FbaI TGATCA 3 cut(s) 469, 3883, 4570
FblI GTMKAC 1 cut(s) 3407
FokI GGATG 9 cut(s) 569, 832, 1100, 1757, 2314, 4248, 4265, 4331, 4585
FriOI GRGCYC 3 cut(s) 3282, 3535, 3933
FspAI RTGCGCAY 1 cut(s) 4560
FspBI CTAG 7 cut(s) 210, 462, 1578, 2309, 2814, 2847, 4679
FspI TGCGCA 2 cut(s) 1709, 4560
GlaI GCGC 4 cut(s) 1055, 1709, 2750, 4560
GsaI CCCAGC 1 cut(s) 2994
GsuI CTGGAG 4 cut(s) 1635, 2658, 2823, 3995
HaeII RGCGCY 1 cut(s) 1057
HapII CCGG 6 cut(s) 275, 450, 673, 1611, 4502, 4602
HgaI GACGC 4 cut(s) 287, 3573, 3956, 4401
HhaI GCGC 4 cut(s) 1056, 1710, 2751, 4561
Hin1I GRCGYC 4 cut(s) 1054, 3584, 3967, 4292
Hin6I GCGC 4 cut(s) 1054, 1708, 2749, 4559
HinP1I GCGC 4 cut(s) 1054, 1708, 2749, 4559
HincII GTYRAC 3 cut(s) 1896, 3453, 4423
HindII GTYRAC 3 cut(s) 1896, 3453, 4423
HindIII AAGCTT 5 cut(s) 341, 2180, 2529, 3383, 4728
HpaI GTTAAC 1 cut(s) 1896
HpaII CCGG 6 cut(s) 275, 450, 673, 1611, 4502, 4602
HphI GGTGA 7 cut(s) 512, 696, 1181, 2041, 2153, 2403, 4302
Hpy166II GTNNAC 9 cut(s) 266, 1896, 2336, 3193, 3408, 3453, 3616, 3891, 4423
Hpy8I GTNNAC 9 cut(s) 266, 1896, 2336, 3193, 3408, 3453, 3616, 3891, 4423
Hpy99I CGWCG 6 cut(s) 143, 146, 687, 1391, 1458, 4297
HpyCH4IV ACGT 4 cut(s) 1987, 3346, 4031, 4292
HpySE526I ACGT 4 cut(s) 1987, 3346, 4031, 4292
Hsp92I GRCGYC 4 cut(s) 1054, 3584, 3967, 4292
HspAI GCGC 4 cut(s) 1054, 1708, 2749, 4559
KasI GGCGCC 1 cut(s) 1053
Kpn2I TCCGGA 1 cut(s) 274
Ksp22I TGATCA 3 cut(s) 469, 3883, 4570
KspAI GTTAAC 1 cut(s) 1896
LguI GCTCTTC 2 cut(s) 1812, 2394
LmnI GCTCC 6 cut(s) 1654, 2804, 3007, 3277, 3522, 4057
MaeI CTAG 7 cut(s) 210, 462, 1578, 2309, 2814, 2847, 4679
MaeII ACGT 4 cut(s) 1987, 3346, 4031, 4292
MbiI CCGCTC 1 cut(s) 1243
MflI RGATCY 5 cut(s) 53, 61, 3501, 3718, 4675
MhlI GDGCHC 6 cut(s) 421, 2938, 3282, 3346, 3535, 3933
MlsI TGGCCA 2 cut(s) 1858, 2041
MluNI TGGCCA 2 cut(s) 1858, 2041
Mly113I GGCGCC 1 cut(s) 1054
MlyI GAGTC 7 cut(s) 452, 1367, 4268, 4754, 4766, 4775, 4784
MmeI TCCRAC 8 cut(s) 234, 665, 1362, 1436, 2062, 4027, 4794, 4812
Mox20I TGGCCA 2 cut(s) 1858, 2041
Mph1103I ATGCAT 2 cut(s) 1764, 3552
MroI TCCGGA 1 cut(s) 274
MroXI GAANNNNTTC 2 cut(s) 618, 2496
MscI TGGCCA 2 cut(s) 1858, 2041
MslI CAYNNNNRTG 6 cut(s) 1062, 2166, 2505, 4021, 4230, 4334
Msp20I TGGCCA 2 cut(s) 1858, 2041
MspA1I CMGCKG 3 cut(s) 424, 1407, 2558
MspCI CTTAAG 3 cut(s) 65, 493, 2588
MspI CCGG 6 cut(s) 275, 450, 673, 1611, 4502, 4602
Mva1269I GAATGC 8 cut(s) 281, 363, 566, 595, 814, 1948, 2369, 3996
MvnI CGCG 2 cut(s) 1444, 2751
NarI GGCGCC 1 cut(s) 1054
NciI CCSGG 2 cut(s) 673, 4502
NcoI CCATGG 1 cut(s) 3933
NdeI CATATG 1 cut(s) 3868
NlaIV GGNNCC 9 cut(s) 1055, 2701, 2806, 3262, 3524, 3533, 3572, 3931, 4163
NmeAIII GCCGAG 1 cut(s) 2680
NmuCI GTSAC 6 cut(s) 702, 938, 2029, 2409, 3317, 4197
NruI TCGCGA 1 cut(s) 1444
NsbI TGCGCA 2 cut(s) 1709, 4560
NsiI ATGCAT 2 cut(s) 1764, 3552
NspI RCATGY 6 cut(s) 605, 768, 946, 1754, 3853, 4619
OliI CACNNNNGTG 1 cut(s) 2505
PaeI GCATGC 3 cut(s) 768, 1754, 4619
PagI TCATGA 3 cut(s) 3915, 4573, 4707
PasI CCCWGGG 2 cut(s) 1008, 4299
PceI AGGCCT 1 cut(s) 527
PciI ACATGT 2 cut(s) 942, 3849
PciSI GCTCTTC 2 cut(s) 1812, 2394
PctI GAATGC 8 cut(s) 281, 363, 566, 595, 814, 1948, 2369, 3996
PdmI GAANNNNTTC 2 cut(s) 618, 2496
PfoI TCCNGGA 3 cut(s) 83, 671, 4500
PleI GAGTC 7 cut(s) 451, 1367, 4268, 4754, 4766, 4774, 4784
PluTI GGCGCC 1 cut(s) 1057
PmaCI CACGTG 1 cut(s) 3347
PmlI CACGTG 1 cut(s) 3347
PpsI GAGTC 7 cut(s) 451, 1367, 4268, 4754, 4766, 4774, 4784
Ppu21I YACGTR 1 cut(s) 3347
PpuMI RGGWCCY 1 cut(s) 2700
PscI ACATGT 2 cut(s) 942, 3849
PshBI ATTAAT 1 cut(s) 2577
Psp124BI GAGCTC 1 cut(s) 3282
Psp5II RGGWCCY 1 cut(s) 2700
PspCI CACGTG 1 cut(s) 3347
PspEI GGTNACC 1 cut(s) 2029
PspFI CCCAGC 1 cut(s) 2990
PspN4I GGNNCC 9 cut(s) 1055, 2701, 2806, 3262, 3524, 3533, 3572, 3931, 4163
PspOMI GGGCCC 2 cut(s) 3531, 3929
PspPPI RGGWCCY 1 cut(s) 2700
PstI CTGCAG 1 cut(s) 4117
PstNI CAGNNNCTG 1 cut(s) 115
PsuI RGATCY 5 cut(s) 53, 61, 3501, 3718, 4675
PvuII CAGCTG 3 cut(s) 424, 1407, 2558
RruI TCGCGA 1 cut(s) 1444
RseI CAYNNNNRTG 6 cut(s) 1062, 2166, 2505, 4021, 4230, 4334
SacI GAGCTC 1 cut(s) 3282
SapI GCTCTTC 2 cut(s) 1812, 2394
ScaI AGTACT 1 cut(s) 3680
SchI GAGTC 7 cut(s) 452, 1367, 4268, 4754, 4766, 4775, 4784
SduI GDGCHC 6 cut(s) 421, 2938, 3282, 3346, 3535, 3933
SfcI CTRYAG 3 cut(s) 2946, 4113, 4266
SfiI GGCCNNNNNGGCC 1 cut(s) 533
SfoI GGCGCC 1 cut(s) 1055
SinI GGWCC 3 cut(s) 1135, 2700, 3113
SmiI ATTTAAAT 1 cut(s) 1933
SmiMI CAYNNNNRTG 6 cut(s) 1062, 2166, 2505, 4021, 4230, 4334
SmlI CTYRAG 7 cut(s) 65, 194, 493, 516, 623, 2588, 3132
SmoI CTYRAG 7 cut(s) 65, 194, 493, 516, 623, 2588, 3132
SpeI ACTAGT 3 cut(s) 209, 461, 2846
SphI GCATGC 3 cut(s) 768, 1754, 4619
SseBI AGGCCT 1 cut(s) 527
SsiI CCGC 5 cut(s) 1243, 2751, 3460, 4235, 4715
SspDI GGCGCC 1 cut(s) 1053
SspI AATATT 1 cut(s) 4492
SspMI CTAG 7 cut(s) 210, 462, 1578, 2309, 2814, 2847, 4679
SstI GAGCTC 1 cut(s) 3282
StuI AGGCCT 1 cut(s) 527
StyI CCWWGG 4 cut(s) 870, 1904, 3933, 4313
SwaI ATTTAAAT 1 cut(s) 1933
TaiI ACGT 4 cut(s) 1990, 3349, 4034, 4295
TaqI TCGA 5 cut(s) 50, 830, 947, 1376, 4402
TaqII GACCGA 2 cut(s) 3130, 3567
TatI WGTACW 6 cut(s) 75, 1723, 1778, 2705, 3011, 3678
TscAI CASTG 9 cut(s) 37, 856, 1543, 1950, 2130, 2614, 3004, 3533, 4816
TseFI GTSAC 6 cut(s) 702, 938, 2029, 2409, 3317, 4197
Tsp45I GTSAC 6 cut(s) 702, 938, 2029, 2409, 3317, 4197
TspGWI ACGGA 1 cut(s) 1507
TspRI CASTG 9 cut(s) 37, 856, 1543, 1950, 2130, 2614, 3004, 3533, 4816
Vha464I CTTAAG 3 cut(s) 65, 493, 2588
VpaK11BI GGWCC 3 cut(s) 1135, 2700, 3113
VspI ATTAAT 1 cut(s) 2577
XagI CCTNNNNNAGG 4 cut(s) 1579, 2812, 3156, 3539
XapI RAATTY 7 cut(s) 36, 1928, 2222, 2290, 2883, 3781, 4208
XbaI TCTAGA 1 cut(s) 1577
XceI RCATGY 6 cut(s) 605, 768, 946, 1754, 3853, 4619
XcmI CCANNNNNNNNNTGG 1 cut(s) 3101
XmiI GTMKAC 1 cut(s) 3407
XmnI GAANNNNTTC 2 cut(s) 618, 2496
XspI CTAG 7 cut(s) 210, 462, 1578, 2309, 2814, 2847, 4679
ZraI GACGTC 1 cut(s) 4293
ZrmI AGTACT 1 cut(s) 3680
Zsp2I ATGCAT 2 cut(s) 1764, 3552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.