Rw6G044800

Enhancer of polycomb-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0010036
Physical Location & Seq
Reverse (-)
67919335 .. 67926243
6909 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G044800.1

Sequence Viewer

Length: 4338 bp
ATGGAAAATAGAATAGAGGTTTCACATGGCACTGAAATTCCCAGGAAATCAAGATCTTTGGATCTTAAGAGTTTGTACAAGTCCAGGAGTAGAAAAGAGGCTGAAAATCAGAGCCTGAAGAGAAAGGGGAGTGAGGACGACGATGGTGGGGACAAGAAGAAGAGGAGTAGGAAAGAGGTGTCTCTCAGTAGTCTCAAGAATGTTAATACTAGTAGTAGCAAGAAGAGTATAGATAAAGCTTATCATAGAGGGTTGGATTCTGGTTCACATGATCCGGACGCATTCAAATCGGGGTCAAGTCAAAAATTGGATAGCAGTAGTGGGTTAAATGGCGTTTCAAAGCTTAGTTTGGATAATAGTGGCATTCAAATCCCAAGGCGTAAAAGGGGATTCGTGGGGCGAAAAAAATGTGAGGGTGCTCAGCCGTTGAAGTTGTCGGATGAGTCTGCCGGTAGAGCAACTAGTGTTGATCAGAATCATCAGATAGCTAACTTAAGTGGTGATGATTTGGGTACTCAAGCTGAGGCCTGGAAGGCCAAAAGAAACAAAGGTTTGGATGAATGCAAAGAAAATATAAATAGTGAGTTGAATGCAACTTCACATGCAAAGAAAGAAAATGTTCTTGAGAGGCAGTCAGTTGCAAGCAATGGTAATTCATCTTTGAAAAAGTCCCAGAGGAATCGCCGGAAAAGGAAGGACTTGTCACCTGATAGTAAAACTGATGCAAAGGAAGCTGAGCCTTTAGTCAATAGTTCTACTAAAGCATGCCAGGCTTCACAAGAAGACGAGGAGGAGAATCTTGAAGAGAATGCAGCGAGGATGCTTTCATCGAGGTTTGATCCAAGCTGTACTGGGTTTTCATTAAACACCAAGGCTTCTGCGATGCAATCTTCAAACGGGTTGTCTAATCAGTCATCTCCTGGTCAGGATTTTGATAGTCACATGTCGAAGTCATTTTCTGGATCAGAATCCCAATCTGTTGATAATGCTGGTAGAGTGCTGAGGCCCAGGGCCAGGAAGCAGCACAAAGAAAAGAAAGGTACGAGGAAGAGGCGCCACTTCTATGAAATCTTCTTCGGGGACTTGGATGCTTGTTGGGTATTGAACCGTAAGATCAAGGTCTTCTGGCCTTTGGACCAAAGTTGGTATTATGGCCTTGTGAATGATTATGACAAGGAGAAAAAACTTCACCATATTAGATACGATGACCGTGAAGAAGAATGGGTTGATCTTCAACATGAGCGGTTCAAGCTCTTGCTACTTCCTACTGAAGTTCCTGGTAAGGCAAAGCAGAAAAAATCAATCATGCAAATTACAAGTTCTGAAGAGAGAGAAGGAAACTTGAAACCCAGAAAAGAGAAGAAAAAGAGAGACTCGATGTCGGAGGACGATAGCTGCATAGGCAGCTGTATGGATTCTGAACCTATTATTTCATGGTTGTCGCGATCTACCCGTCGGATCAAGTCTCCTTCTCATGCTGTGAAGAAGCAAAAAACAACTGGTCTATCTCCAAAGTCCGTGCCAACTTTGTCTGACAGTGCTGGCACTTTACATGGGTGTTTGAGTGATGTTTCCTCTAGAAGGGATACAAATAAATTATCTTGTAATTCCGGCAGATCATCTGATCCTGTGAGGGAAGAGAAGCCTGCACCAGAAGGCAACTCCGAAGATAGCAAAATGCCTATTGTTTATTATCGGAAACGATTGCGCAAGACTGGCTCTGTACTGTCTCAAATATACAAGGATGAGCATGCTTCCATGCATGGACATCATTGTTGTACTTCTGTTACTCCTGTTGAGGAGGTTTGGGACTTGGAAGAGCCGGATGATTTTGTTGTTATATTGGATCGCAGTTGGCCATTGTGGTATAGTGATGATGCAGGGTTGCTAAAGTTAACTCTTCCTTGGATAGAATCTGGAAAAGTCAAATTTAAATGCCTTCCACTGCATCCACTAATGAATGATTCTCTTGGAGTAGAGTTGTTACGGTTCTTTCAAGCTGCAATGCTGCTTCGGCATGGTGTAGTGGTGACCACATGGCCAAAGGTTCATTTGGAGATGCTCTTTGTTGATAATGTAGTTGGACTGAGGTTTCTTTTGTTTGAAGGTTGCTTGAAGCAGGCGGTGGCCTTTGTTTCTGTTATCCTGACATTATTTCATCACCCTAATGAACAGGGGAAGCTTACTGATTTCCAGTTACCAGCAACTTCAATCAGGTTTAAATTCTCCTGTGTTCAGCATCTTGGAAAGCAACTCGTGTCTGCTTTCTACAACTTCTGTCAAGTTAAAAATTCAACATGGATGTACCTAGACAATAAACTCAGGAGGCATTGTTTACTCGCCAAAAAACTGCCTCTGTCTGAATGCACCTATGATAATATCCAGGCACTTCAAAATGGAATAAATCAGTCACCTTCTATGTCTCTTTGCTGGCAACCCTCTGTCAAGGGTACACGGAAGAGGTCCAGGCAGGGCATTAACTTCATGGGCAGTTCTCGGGAAGTTAGTTTTGTAAATATCAGCCATTCTGCTACTCATTCTGATGAGCTTCACAGAAAGCTTCCCCCACTTGCTCTATCTTTTACAGCTGCACCTACTTTCTTCATTAATTTGCATCTTAAGTTGCTTATGGAACACAGTGTTGCTAATATCTGCTTTCAAGACCATGATTCAGAAATTACTCCCGAGAACAATTTGAAGGCTCCAGCAAATGTGGCTACCTCTGGTGGGTCCTGTACTAAAGTGGTAACAGAAACATCTCTTTCTATTTGCAGTCAGCGCGGTCGGTTAAAGTCATCTCAGCTTTACCAAAATTGTGTTGTTAATGTTGCTGGAGCCTCTTCTAGGACTGTCACTGGCAGAGATAAATCCGAAACTAGTTCCCGTTCTATTGTGAATGGGCTTACTGTTGAAATTCCATCTTTTGACCATTTTGAGAAGCCTGTGGAGAGGGAGTTACAAAATGCTCAACAGCCTACAGTTTTTTCATTGAATATGAATGGTAGCATTATCCCCAGTCCCAGCCCCACTGCTCCCAGAAGTACAGGGCAGCGAAATAGAAGTAGTATGTCATCATTTGGAAACCTCGCACATCGTTGGTCAGATGGAAAGGCTGACATTTTCCATAATGGTTTTGGTAATGGACCGAAGAAGCCCCGAACTCAAGTCTCATACACATTGCCTTTTGGAGGTTTTGATGTCAGCTCTAAGCAAAGAAATGTTCACAAAGGGCTTCCTAATAAGCGAATTAGAAGGGCCAGCGAGAAGAGGTCATCAGACATTTCCAGAGGTTCCGAAAGGAACTTGGAGCTCTTATCCTGTGAGGCAAATGTGTTAATTTCTGCAAGTGACAGAGGGTGGAGAGAATGTGGGGCACGTGTAGTATTAGAACTCTTTGATAGTAGTGAGTGGAAGCTTGCTGTAAAACTTTCAGGGACTACGAAGTATTCATACAAAGCACATCAATTTTTGCAGCCTGGGTCAACAAACCGCTATACTCATGTTATGATGTGGAAAGGAGGAAAGGATTGGATCTTGGAGTTTCCAGATAGGAGCCAGTGGGCCCTTTTCAAGGAGATGCATGAAGAGTGTTACAATCGGAACCTTCGGTCTGCGTCAGTTAAAAACATTCCTATTCCTGGTGTTCGCTTAGTAGAAGATATTGATGATAATGGAACAGAAATAGCATTTCTTCGTAGTTCTACCAAGTACTTTCAGCAGATGAAAACAGATGTTGAGATGGCTTTAGATCCATCTCGTCTCTTGTATGACATGGATAGTGATGATGAGCGATGGATTTTGAAGTTTCAAAATTCTTCAGAAGTTGAAAAGAGTAGCTCAATGGAGATTGGCGAGGAGATGTTTGAGAAGACAATGGACATGTTTGAGAAGGCTGCATATGTTCGGCAATGTGATCAGTTCACATCTGAAGAAATAGAAGAGTTCATGACTGGTATTGGGCCCATGGATTTGATTAAAACCATTTATGAGCATTGGCGTCAGAAACGGCTGAGAAAGGGAATGCCTTTAATCCGACATCTCCAGCCACCGTCATGGGAGACGTATCAAAAACAAGTGAGGGAGTGGGAGCAAGCTATGACCAAGATGAATAACACACTCGCAAATGGTGGCCGTGAGAAAGCTGCAGCAGTTGAGAAGCCACCGATGTATGCTTTTTGTCTGAAACCACGGGGCCTGGAAGTTCCCAATAAGGGATCAAAGCAACGGTCACAGAAGAAATTTTCTATTTCTGCACATACCAATGCGGTGTTAGGAGATCAGGATGGTTTCCATCCTATTGGTAACTTTTTTTTACCTCATTTAAGACGTAAATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPL1

Protein Analysis

1445

Amino Acids

163.38

Weight (kDa)

9.3

Isoelectric Point (pI)

57.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPL1 PF10513 1202 - 1297 1.4e-08 Enhancer of polycomb-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1709
AccB1I GGYRCC 1 cut(s) 1053
AccBSI CCGCTC 1 cut(s) 1243
AccII CGCG 2 cut(s) 1444, 2781
AccIII TCCGGA 1 cut(s) 274
AciI CCGC 5 cut(s) 1243, 2123, 2781, 3490, 4265
AcoI YGGCCR 3 cut(s) 1856, 2039, 4129
AcsI RAATTY 7 cut(s) 36, 1928, 2222, 2290, 2913, 3811, 4238
AcuI CTGAAG 5 cut(s) 137, 1290, 1344, 3801, 3948
AcvI CACGTG 1 cut(s) 3377
AcyI GRCGYC 2 cut(s) 1054, 3997
AfiI CCNNNNNNNGG 8 cut(s) 1014, 1581, 2727, 2844, 3188, 3571, 3638, 4211
AflII CTTAAG 3 cut(s) 65, 493, 2618
AflIII ACRYGT 3 cut(s) 942, 3376, 3879
AhdI GACNNNNNGTC 1 cut(s) 1378
AhlI ACTAGT 3 cut(s) 209, 461, 2876
Alw21I GWGCWC 2 cut(s) 421, 3312
Alw26I GTCTC 9 cut(s) 186, 197, 1365, 1470, 1734, 2427, 3172, 3764, 4052
AlwNI CAGNNNCTG 1 cut(s) 115
Ama87I CYCGRG 2 cut(s) 2496, 2684
Aor13HI TCCGGA 1 cut(s) 274
ApaI GGGCCC 2 cut(s) 3565, 3963
ApoI RAATTY 7 cut(s) 36, 1928, 2222, 2290, 2913, 3811, 4238
ArsI GACNNNNNNTTYG 4 cut(s) 887, 919, 4211, 4243
AseI ATTAAT 1 cut(s) 2607
Asp700I GAANNNNTTC 1 cut(s) 618
AspLEI GCGC 3 cut(s) 1056, 1710, 2781
AsuHPI GGTGA 6 cut(s) 512, 696, 1181, 2041, 2153, 2403
AvaI CYCGRG 2 cut(s) 2496, 2684
AvaII GGWCC 4 cut(s) 1135, 2463, 2730, 3143
BaeGI GKGCMC 3 cut(s) 3376, 3565, 3963
BalI TGGCCA 2 cut(s) 1858, 2041
BanI GGYRCC 1 cut(s) 1053
BanII GRGCYC 3 cut(s) 3312, 3565, 3963
BarI GAAGNNNNNNTAC 2 cut(s) 3434, 3466
BauI CACGAG 1 cut(s) 2255
BbrPI CACGTG 1 cut(s) 3377
BbsI GAAGAC 3 cut(s) 789, 1114, 3875
Bbv12I GWGCWC 2 cut(s) 421, 3312
BbvCI CCTCAGC 2 cut(s) 522, 1001
BccI CCATC 8 cut(s) 137, 2926, 3098, 3733, 3760, 3786, 4277, 4299
BceAI ACGGC 3 cut(s) 409, 4022, 4116
BcgI CGANNNNNNTGC 2 cut(s) 270, 304
BciVI GTATCC 1 cut(s) 1579
BclI TGATCA 2 cut(s) 469, 3913
BcoDI GTCTC 9 cut(s) 186, 197, 1365, 1470, 1734, 2427, 3172, 3764, 4052
BcuI ACTAGT 3 cut(s) 209, 461, 2876
BfaI CTAG 6 cut(s) 210, 462, 1578, 2309, 2844, 2877
BfmI CTRYAG 2 cut(s) 2976, 4143
BfoI RGCGCY 1 cut(s) 1057
BfrI CTTAAG 3 cut(s) 65, 493, 2618
BfuI GTATCC 1 cut(s) 1579
BglI GCCNNNNNGGC 1 cut(s) 533
BglII AGATCT 1 cut(s) 53
BlpI GCTNAGC 2 cut(s) 420, 735
BmcAI AGTACT 1 cut(s) 3710
Bme18I GGWCC 4 cut(s) 1135, 2463, 2730, 3143
BmeRI GACNNNNNGTC 1 cut(s) 1378
BmeT110I CYCGRG 2 cut(s) 2496, 2684
BmrI ACTGGG 2 cut(s) 861, 3009
BmuI ACTGGG 2 cut(s) 861, 3009
BpiI GAAGAC 3 cut(s) 789, 1114, 3875
BpmI CTGGAG 3 cut(s) 2688, 2853, 4025
Bpu10I CCTNAGC 2 cut(s) 522, 1001
Bpu1102I GCTNAGC 2 cut(s) 420, 735
BpuEI CTTGAG 4 cut(s) 179, 501, 644, 3147
BsaAI YACGTR 1 cut(s) 3377
BsaBI GATNNNNATC 2 cut(s) 474, 967
BsaHI GRCGYC 2 cut(s) 1054, 3997
BsaJI CCNNGG 9 cut(s) 41, 374, 870, 1007, 1008, 1904, 3476, 3963, 4187
BsaWI WCCGGW 1 cut(s) 274
BsaXI ACNNNNNCTCC 6 cut(s) 2317, 2347, 2939, 2969, 3352, 3382
Bsc4I CCNNNNNNNGG 8 cut(s) 1014, 1581, 2727, 2844, 3188, 3571, 3638, 4211
Bse118I RCCGGY 1 cut(s) 449
Bse1I ACTGG 8 cut(s) 856, 1504, 1720, 2194, 2860, 3015, 3556, 3955
Bse3DI GCAATG 4 cut(s) 652, 2010, 3176, 3914
Bse8I GATNNNNATC 2 cut(s) 474, 967
BseAI TCCGGA 1 cut(s) 274
BseDI CCNNGG 9 cut(s) 41, 374, 870, 1007, 1008, 1904, 3476, 3963, 4187
BseJI GATNNNNATC 2 cut(s) 474, 967
BseLI CCNNNNNNNGG 8 cut(s) 1014, 1581, 2727, 2844, 3188, 3571, 3638, 4211
BseMI GCAATG 4 cut(s) 652, 2010, 3176, 3914
BseMII CTCAG 9 cut(s) 199, 434, 513, 726, 992, 2078, 2335, 2813, 4001
BseNI ACTGG 8 cut(s) 856, 1504, 1720, 2194, 2860, 3015, 3556, 3955
BseRI GAGGAG 5 cut(s) 178, 803, 806, 1814, 3869
BseSI GKGCMC 3 cut(s) 3376, 3565, 3963
BseYI CCCAGC 1 cut(s) 3020
BsgI GTGCAG 3 cut(s) 1632, 2574, 4236
Bsh1236I CGCG 2 cut(s) 1444, 2781
Bsh1285I CGRYCG 1 cut(s) 2785
BshNI GGYRCC 1 cut(s) 1053
BsiEI CGRYCG 1 cut(s) 2785
BsiHKAI GWGCWC 2 cut(s) 421, 3312
BsiHKCI CYCGRG 2 cut(s) 2496, 2684
BsiSI CCGG 5 cut(s) 275, 450, 685, 1611, 1823
BslFI GGGAC 6 cut(s) 164, 655, 1094, 1823, 3003, 3448
BslI CCNNNNNNNGG 8 cut(s) 1014, 1581, 2727, 2844, 3188, 3571, 3638, 4211
BsmAI GTCTC 9 cut(s) 186, 197, 1365, 1470, 1734, 2427, 3172, 3764, 4052
BsmBI CGTCTC 2 cut(s) 3764, 4052
BsmFI GGGAC 6 cut(s) 164, 655, 1094, 1823, 3003, 3448
BsmI GAATGC 7 cut(s) 281, 363, 566, 595, 814, 2369, 4026
BsoBI CYCGRG 2 cut(s) 2496, 2684
Bsp120I GGGCCC 2 cut(s) 3561, 3959
Bsp1286I GDGCHC 5 cut(s) 421, 3312, 3376, 3565, 3963
Bsp13I TCCGGA 1 cut(s) 274
Bsp1407I TGTACA 1 cut(s) 75
Bsp1720I GCTNAGC 2 cut(s) 420, 735
Bsp19I CCATGG 1 cut(s) 3963
Bsp68I TCGCGA 1 cut(s) 1444
BspACI CCGC 5 cut(s) 1243, 2123, 2781, 3490, 4265
BspCNI CTCAG 9 cut(s) 198, 433, 514, 727, 993, 2079, 2334, 2812, 4002
BspEI TCCGGA 1 cut(s) 274
BspFNI CGCG 2 cut(s) 1444, 2781
BspHI TCATGA 1 cut(s) 3945
BspMAI CTGCAG 1 cut(s) 4147
BspQI GCTCTTC 1 cut(s) 1812
BspT107I GGYRCC 1 cut(s) 1053
BspTI CTTAAG 3 cut(s) 65, 493, 2618
BsrBI CCGCTC 1 cut(s) 1243
BsrDI GCAATG 4 cut(s) 652, 2010, 3176, 3914
BsrFI RCCGGY 1 cut(s) 449
BsrGI TGTACA 1 cut(s) 75
BsrI ACTGG 8 cut(s) 856, 1504, 1720, 2194, 2860, 3015, 3556, 3955
BssAI RCCGGY 1 cut(s) 449
BssECI CCNNGG 9 cut(s) 41, 374, 870, 1007, 1008, 1904, 3476, 3963, 4187
BssNI GRCGYC 2 cut(s) 1054, 3997
BssSI CACGAG 1 cut(s) 2255
BssT1I CCWWGG 4 cut(s) 374, 870, 1904, 3963
Bst2BI CACGAG 1 cut(s) 2255
BstACI GRCGYC 2 cut(s) 1054, 3997
BstAFI CTTAAG 3 cut(s) 65, 493, 2618
BstAUI TGTACA 1 cut(s) 75
BstBAI YACGTR 1 cut(s) 3377
BstDSI CCRYGG 2 cut(s) 3963, 4187
BstEII GGTNACC 1 cut(s) 2029
BstENI CCTNNNNNAGG 4 cut(s) 1579, 2842, 3186, 3569
BstFNI CGCG 2 cut(s) 1444, 2781
BstH2I RGCGCY 1 cut(s) 1057
BstHHI GCGC 3 cut(s) 1056, 1710, 2781
BstMAI GTCTC 9 cut(s) 186, 197, 1365, 1470, 1734, 2427, 3172, 3764, 4052
BstMCI CGRYCG 1 cut(s) 2785
BstNSI RCATGY 5 cut(s) 605, 768, 946, 1754, 3883
BstPI GGTNACC 1 cut(s) 2029
BstSFI CTRYAG 2 cut(s) 2976, 4143
BstSLI GKGCMC 3 cut(s) 3376, 3565, 3963
BstUI CGCG 2 cut(s) 1444, 2781
BstV2I GAAGAC 3 cut(s) 789, 1114, 3875
BstX2I RGATCY 4 cut(s) 53, 61, 3531, 3748
BstXI CCANNNNNNTGG 2 cut(s) 4053, 4298
BstYI RGATCY 4 cut(s) 53, 61, 3531, 3748
BsuI GTATCC 1 cut(s) 1579
BtgI CCRYGG 2 cut(s) 3963, 4187
BtgZI GCGATG 2 cut(s) 896, 3805
BtsI GCAGTG 2 cut(s) 1943, 3027
BtsIMutI CAGTG 7 cut(s) 30, 1543, 1943, 2644, 2853, 3027, 3563
BtuMI TCGCGA 1 cut(s) 1444
CaiI CAGNNNCTG 1 cut(s) 115
CciI TCATGA 1 cut(s) 3945
CfoI GCGC 3 cut(s) 1056, 1710, 2781
Cfr10I RCCGGY 1 cut(s) 449
CseI GACGC 3 cut(s) 287, 3603, 3986
DinI GGCGCC 1 cut(s) 1055
DraI TTTAAA 2 cut(s) 1933, 2221
DriI GACNNNNNGTC 1 cut(s) 1378
EaeI YGGCCR 3 cut(s) 1856, 2039, 4129
Eam1105I GACNNNNNGTC 1 cut(s) 1378
Ecl136II GAGCTC 1 cut(s) 3310
Eco130I CCWWGG 4 cut(s) 374, 870, 1904, 3963
Eco147I AGGCCT 1 cut(s) 527
Eco24I GRGCYC 3 cut(s) 3312, 3565, 3963
Eco47I GGWCC 4 cut(s) 1135, 2463, 2730, 3143
Eco53kI GAGCTC 1 cut(s) 3310
Eco57I CTGAAG 5 cut(s) 137, 1290, 1344, 3801, 3948
Eco72I CACGTG 1 cut(s) 3377
Eco88I CYCGRG 2 cut(s) 2496, 2684
Eco91I GGTNACC 1 cut(s) 2029
EcoICRI GAGCTC 1 cut(s) 3310
EcoNI CCTNNNNNAGG 4 cut(s) 1579, 2842, 3186, 3569
EcoO109I RGGNCCY 3 cut(s) 2730, 3562, 4192
EcoO65I GGTNACC 1 cut(s) 2029
EcoT14I CCWWGG 4 cut(s) 374, 870, 1904, 3963
EcoT22I ATGCAT 2 cut(s) 1764, 3582
EcoT38I GRGCYC 3 cut(s) 3312, 3565, 3963
EgeI GGCGCC 1 cut(s) 1055
EheI GGCGCC 1 cut(s) 1055
ErhI CCWWGG 4 cut(s) 374, 870, 1904, 3963
Esp3I CGTCTC 2 cut(s) 3764, 4052
FaqI GGGAC 6 cut(s) 164, 655, 1094, 1823, 3003, 3448
FauNDI CATATG 1 cut(s) 3898
FbaI TGATCA 2 cut(s) 469, 3913
FriOI GRGCYC 3 cut(s) 3312, 3565, 3963
FspBI CTAG 6 cut(s) 210, 462, 1578, 2309, 2844, 2877
FspI TGCGCA 1 cut(s) 1709
GlaI GCGC 3 cut(s) 1055, 1709, 2780
GsaI CCCAGC 1 cut(s) 3024
GsuI CTGGAG 3 cut(s) 2688, 2853, 4025
HaeII RGCGCY 1 cut(s) 1057
HapII CCGG 5 cut(s) 275, 450, 685, 1611, 1823
HgaI GACGC 3 cut(s) 287, 3603, 3986
HhaI GCGC 3 cut(s) 1056, 1710, 2781
Hin1I GRCGYC 2 cut(s) 1054, 3997
Hin6I GCGC 3 cut(s) 1054, 1708, 2779
HinP1I GCGC 3 cut(s) 1054, 1708, 2779
HincII GTYRAC 2 cut(s) 1896, 3483
HindII GTYRAC 2 cut(s) 1896, 3483
HindIII AAGCTT 5 cut(s) 237, 341, 2180, 2558, 3413
HpaI GTTAAC 1 cut(s) 1896
HpaII CCGG 5 cut(s) 275, 450, 685, 1611, 1823
HphI GGTGA 6 cut(s) 512, 696, 1181, 2041, 2153, 2403
Hpy166II GTNNAC 7 cut(s) 266, 1896, 2336, 2453, 3223, 3483, 3921
Hpy8I GTNNAC 7 cut(s) 266, 1896, 2336, 2453, 3223, 3483, 3921
Hpy99I CGWCG 2 cut(s) 143, 1458
HpyCH4IV ACGT 3 cut(s) 3376, 4061, 4327
HpySE526I ACGT 3 cut(s) 3376, 4061, 4327
Hsp92I GRCGYC 2 cut(s) 1054, 3997
HspAI GCGC 3 cut(s) 1054, 1708, 2779
KasI GGCGCC 1 cut(s) 1053
Kpn2I TCCGGA 1 cut(s) 274
Ksp22I TGATCA 2 cut(s) 469, 3913
KspAI GTTAAC 1 cut(s) 1896
LguI GCTCTTC 1 cut(s) 1812
LmnI GCTCC 6 cut(s) 2707, 2834, 3037, 3307, 3552, 4087
MaeI CTAG 6 cut(s) 210, 462, 1578, 2309, 2844, 2877
MaeII ACGT 3 cut(s) 3376, 4061, 4327
MbiI CCGCTC 1 cut(s) 1243
MflI RGATCY 4 cut(s) 53, 61, 3531, 3748
MhlI GDGCHC 5 cut(s) 421, 3312, 3376, 3565, 3963
MlsI TGGCCA 2 cut(s) 1858, 2041
MluNI TGGCCA 2 cut(s) 1858, 2041
Mly113I GGCGCC 1 cut(s) 1054
MlyI GAGTC 2 cut(s) 452, 1367
MmeI TCCRAC 6 cut(s) 234, 417, 1362, 1436, 2062, 4057
Mox20I TGGCCA 2 cut(s) 1858, 2041
Mph1103I ATGCAT 2 cut(s) 1764, 3582
MroI TCCGGA 1 cut(s) 274
MroXI GAANNNNTTC 1 cut(s) 618
MscI TGGCCA 2 cut(s) 1858, 2041
MslI CAYNNNNRTG 5 cut(s) 1062, 2166, 2541, 4051, 4260
Msp20I TGGCCA 2 cut(s) 1858, 2041
MspA1I CMGCKG 2 cut(s) 1407, 2588
MspCI CTTAAG 3 cut(s) 65, 493, 2618
MspI CCGG 5 cut(s) 275, 450, 685, 1611, 1823
Mva1269I GAATGC 7 cut(s) 281, 363, 566, 595, 814, 2369, 4026
MvnI CGCG 2 cut(s) 1444, 2781
NarI GGCGCC 1 cut(s) 1054
NcoI CCATGG 1 cut(s) 3963
NdeI CATATG 1 cut(s) 3898
NmuCI GTSAC 7 cut(s) 702, 938, 2029, 2409, 2851, 3347, 4227
NruI TCGCGA 1 cut(s) 1444
NsbI TGCGCA 1 cut(s) 1709
NsiI ATGCAT 2 cut(s) 1764, 3582
NspI RCATGY 5 cut(s) 605, 768, 946, 1754, 3883
PaeI GCATGC 2 cut(s) 768, 1754
PagI TCATGA 1 cut(s) 3945
PasI CCCWGGG 1 cut(s) 1008
PceI AGGCCT 1 cut(s) 527
PciI ACATGT 2 cut(s) 942, 3879
PciSI GCTCTTC 1 cut(s) 1812
PctI GAATGC 7 cut(s) 281, 363, 566, 595, 814, 2369, 4026
PdmI GAANNNNTTC 1 cut(s) 618
PfoI TCCNGGA 1 cut(s) 83
PleI GAGTC 2 cut(s) 451, 1367
PluTI GGCGCC 1 cut(s) 1057
PmaCI CACGTG 1 cut(s) 3377
PmlI CACGTG 1 cut(s) 3377
PpsI GAGTC 2 cut(s) 451, 1367
Ppu21I YACGTR 1 cut(s) 3377
PpuMI RGGWCCY 1 cut(s) 2730
PscI ACATGT 2 cut(s) 942, 3879
PshBI ATTAAT 1 cut(s) 2607
Psp124BI GAGCTC 1 cut(s) 3312
Psp5II RGGWCCY 1 cut(s) 2730
PspCI CACGTG 1 cut(s) 3377
PspEI GGTNACC 1 cut(s) 2029
PspFI CCCAGC 1 cut(s) 3020
PspOMI GGGCCC 2 cut(s) 3561, 3959
PspPPI RGGWCCY 1 cut(s) 2730
PstI CTGCAG 1 cut(s) 4147
PstNI CAGNNNCTG 1 cut(s) 115
PsuI RGATCY 4 cut(s) 53, 61, 3531, 3748
PvuII CAGCTG 2 cut(s) 1407, 2588
RruI TCGCGA 1 cut(s) 1444
RseI CAYNNNNRTG 5 cut(s) 1062, 2166, 2541, 4051, 4260
SacI GAGCTC 1 cut(s) 3312
SapI GCTCTTC 1 cut(s) 1812
ScaI AGTACT 1 cut(s) 3710
SchI GAGTC 2 cut(s) 452, 1367
SduI GDGCHC 5 cut(s) 421, 3312, 3376, 3565, 3963
SfcI CTRYAG 2 cut(s) 2976, 4143
SfiI GGCCNNNNNGGCC 1 cut(s) 533
SfoI GGCGCC 1 cut(s) 1055
SinI GGWCC 4 cut(s) 1135, 2463, 2730, 3143
SmiI ATTTAAAT 1 cut(s) 1933
SmiMI CAYNNNNRTG 5 cut(s) 1062, 2166, 2541, 4051, 4260
SmlI CTYRAG 7 cut(s) 65, 194, 493, 516, 623, 2618, 3162
SmoI CTYRAG 7 cut(s) 65, 194, 493, 516, 623, 2618, 3162
SpeI ACTAGT 3 cut(s) 209, 461, 2876
SphI GCATGC 2 cut(s) 768, 1754
SseBI AGGCCT 1 cut(s) 527
SsiI CCGC 5 cut(s) 1243, 2123, 2781, 3490, 4265
SspDI GGCGCC 1 cut(s) 1053
SspMI CTAG 6 cut(s) 210, 462, 1578, 2309, 2844, 2877
SstI GAGCTC 1 cut(s) 3312
StuI AGGCCT 1 cut(s) 527
StyI CCWWGG 4 cut(s) 374, 870, 1904, 3963
SwaI ATTTAAAT 1 cut(s) 1933
TaiI ACGT 3 cut(s) 3379, 4064, 4330
TaqI TCGA 3 cut(s) 830, 947, 1376
TaqII GACCGA 2 cut(s) 3160, 3597
TatI WGTACW 7 cut(s) 75, 848, 1723, 1778, 2735, 3041, 3708
TscAI CASTG 7 cut(s) 37, 1543, 1950, 2644, 2860, 3034, 3563
TseFI GTSAC 7 cut(s) 702, 938, 2029, 2409, 2851, 3347, 4227
Tsp45I GTSAC 7 cut(s) 702, 938, 2029, 2409, 2851, 3347, 4227
TspGWI ACGGA 2 cut(s) 1507, 2470
TspRI CASTG 7 cut(s) 37, 1543, 1950, 2644, 2860, 3034, 3563
Vha464I CTTAAG 3 cut(s) 65, 493, 2618
VpaK11BI GGWCC 4 cut(s) 1135, 2463, 2730, 3143
VspI ATTAAT 1 cut(s) 2607
XagI CCTNNNNNAGG 4 cut(s) 1579, 2842, 3186, 3569
XapI RAATTY 7 cut(s) 36, 1928, 2222, 2290, 2913, 3811, 4238
XbaI TCTAGA 1 cut(s) 1577
XceI RCATGY 5 cut(s) 605, 768, 946, 1754, 3883
XcmI CCANNNNNNNNNTGG 1 cut(s) 3131
XmnI GAANNNNTTC 1 cut(s) 618
XspI CTAG 6 cut(s) 210, 462, 1578, 2309, 2844, 2877
ZrmI AGTACT 1 cut(s) 3710
Zsp2I ATGCAT 2 cut(s) 1764, 3582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.