Rh6AG515600

Enhancer of polycomb-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0010036
Physical Location & Seq
Reverse (-)
68542375 .. 68549439
7065 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG515600.1

Sequence Viewer

Length: 4941 bp
ATGGAAAATAGAATAGAGGTTTCACATGGCACTGAAATTCCCAGGAAATCGAGATCTTTGGATCTTAAGAGTTTGTACAAGTCCAGGAGTAGAAAAGAGGCTGAAAATCAGAGCCTGAAGAGAAAAGGGAGTGAGGACGACGACGGTGGGGACAAGAAGAAGAGGAGTAGGAAAGAGGTGTCTCTCAGTAGTCTCAAGAATGTTAATACTAGTAGTAGCAAGAAGAGTATAGATAAAGCTTATCATAGAGGGTTGGATTCTGGTTCACATGATCCGGACGCATTCAAATCGGGGTCAAGTCAAAAATTGGATAGCAGTAGTGGGTTAAATGGCGTTTCAAAGCTTAGTTTGGATAATAGTGGCATTCAAATCCCGAGGCGTAAGAGGGGATTCGTGGGGCGAAAAAAATGTGAGGGTGCTCAGCTGTTGAAGCTGTCAGATGAGTCTGCCGGTAGAGCAACTAGTGTTGATCAGAATCATCAGATAGCTAACTTAAGTGGTGATGATTTGAGTACTCAAGCTGAGGCCTGGAAGGCCAAAAGAAACAAAGGTTTGGATGAATGCAAAGAAAATATAAATAGTGAGTTGAATGCAACTTCACATGCAAAGAAAGAAAATGTTCTTGAGAGGCAGTCCGTTGCAAACAATGGTAATTCATCTTTGAAAAAGTCCCGGAGGAATCGTCGGAAAAGGAAGGACTTGTCACCTGATAGTAAAACTGATGCCAAGGAAGCTGAGCCTTTAGTCAATAGTTCTACTAAAGCATGCCAGGCTTCACAAGAAGACGAGGAGGAGAATCTTGAAGAGAATGCAGCGAGGATGCTTTCATCGAGGTTTGATCCAAGCTGTACTGGGTTTTCATTAAACACCAAGGCTTCTGCGATGCAATCTTCAAACGGGTTGTCTAATCAGTCATCTCCTGGTCAGGATTTTGATAGTCACATGTCGAAGTCATTTTCTGGATCAGAATCTCAATCTGTTGATAATGCTGGTAGAGTGCTGAGGCCCAGGGCCAGGAAGCAGCACAAAGAAAAGAAAGGTACGAGGAAGAGGCGCCACTTCTATGAAATCTTCTTTGGGGACTTGGATGCTTGTTGGGTATTGAACCGTAAGATCAAGGTCTTCTGGCCTTTGGACCAAAGTTGGTATTATGGCCTTGTGAATGATTATGACAAGGAGAAAAAACTTCACCATATTAGATACGATGACCGTGAAGAAGAATGGGTTGATCTTCAACATGAGCGGTTCAAGCTCTTGCTACTTCCTACTGAAGTTCCTGGTAAGGCAAAACAGAAAAAATCAATCATGCAAATTACAAGTTCTGAAGAGAGAGAAGGAAACTTGAAACCCAGAAAAGAGAAGAAAAAGAGAGACTCGATGTCGGAGGACGATAGCTGCATAGGCAGCTGTATGGATTCTGAACCTATTATTTCATGGTTGTCGCGATCTACCCGTCGGAGCAAGTCTCCTTCTCATGCTGTGAAGAAGCAAAAAACAACTGGTCTATCTCCAAAGTCCGTGCCAACTTTGTCTGACAGTGCTGGCACTTTACATGGGTGTTTGAGTGATGTTTCCTCTAGAAGGGATACAAATAAATTATCTTGTAATTCCGGCAGATCATCTGATCCTGTGAGGGAAGAGAAGCCTGCTCCAGAAGGCAACTCCGAAGATAGCAAAATGCCTATTGTTTATTATCGGAAGCGATTGCGCAAGACTGGCTCTGTACTGTCTCAAATATACAAGGATGAGCATGCTTCCATGCATGGACATCATTGTTGTACTTCTGTTACTCCTGTTGAGGAGGTTTGGGACTTGGAAGAGCCGGATGATTTTGTTGTTATATTTGATCGCAGTTGGCCATTGTGGTATAGTGATGATGCAGGGTTGCTAAAGTTAACTCTTCCTTGGATAGAATCTGGAAAAGTCAAATTTAAATGCCTTCCACTGCATTCACTAATGAATGATTCTCTTGGAGTAGAGTTGTTACGGTTCTTTCAAGCTGCAATGCTACTTCGGCATGGTGTAGTGGTGACCACATGGCCAAAGGTTCATTTGGAGATGCTCTTTGTTGATAATGTAGTTGGACTGAGGTTTCTTTTGTTTGAAGGTTGCTTGAAGCAGGCGGTGGCCTTTGTTTCTCTTATCCTGACATTATTTCATCACCCTAATGAACAGGGGAAGCTTACTGATTTCCAGTTACCAGCAACTTCAATCAGGTTTAAATTCTCCTGTGTTCAGCATCTTGGAAAGCAACTTGTGTCTGCTTTCTACAACTTCTGTCAAGTTAAAAATTCAACATGGATGTACCTAGACAATAAACTCAGGAGGCATTGTTTACTCGCCAAAAAACTGCCTCTGTCTGAATGCACCTATGATAATATCCAGGCACTTCAAAATGGAATAAATCAGTCACCTTCTATGTCTCTTTGCAGGCAACCCTCTGTCAAGGGTACACGGAAGAGGTCCAGGCAGGGCATTAACTTCATGGGCAGTTCTCGGGAAGTTAGTTTTGTAAATATCAGCCATTCTGCTACTCATTCTGATGAGCTTCACAGAAAGCTTCCCCCACTTGCTCTATCTTTTACAGCTGCACCTACTTTCTTCATTAATTTGCATCTTAAGTTGCTTATGGAACACAGTGTTGCTAATATCTGCTTTCAAGACCATGATTCAGAAATTACTCCCGAGAACAATTTGAAGGCTCCCGCAAATGTGGCTACCTCTGGTGGGTCCTGTACTAAAGTGGTAACAGAAACATCTCTTTCTATTTGCAGTCAGCGCGGTCGGTTAAAGTCATCTCAGCTTTACCAAAATTGTGTTGTTAATGTTGCTGGAGCCTCTTCTAGGACTTTCACTGGCAGAGATAAATCTGAAACTAGTTCCCGTTCTATTGTGAATGGGCTTACTGTTGAAATTCCATCTTTTGACCATTTTGAGAAGCCTGTGGAGAGGGAGTTACAAAGTGCCCAACAGCCTACAGATTTTCAACAGCCTACAGTTTTTTCATTGAATATGAATGGTAGCATTATCCCCAGTCCCAGCCCCACTGCTCCCAGAAGTACAGGGCAGCGAAATAGAAGTAGTATGTCATCATTTGGAAACCTCGCACATCGTTGGTCAGATGGAAAGGCTGACATTTTCCATAATGGTTTTGGTAATGGACCGAAGAAGCCCCGAACTCAAGTCTCATACACATTGCCTTGTGGAGGTTTTGATGTCAGCTCTAAGCAAAGAAATGTTCACAAAGGGCTTCCTAATAAGCGAATTAGAAGGGCCAGCGAGAAGAGGTCATCAGACATTTCCAGAGGTTCCGAAAGGAACTTGGAGCTCTTATCCTGTGAGGCAAATGTGTTAATTTCTGCAAGTGACAGAGGGTGGAGAGAATGTGGGGCACGTGTAGTATTAGAACTCTTTGATAATAGTGAGTGGAAGCTTGCTGTAAAACTTTCAGGGACTACGAAGTATTCATACAAAGCACATCAATTTTTGCAGCCTGGGTCAACAAACCGCTATACTCATGTTATGATGTGGAAAGGAGGAAAGGATTGGATCTTGGAGTTTCCAGATAGGAGCCAGTGGGCCCTTTTCAAGGAGATGCATGAAGAGTGTTACAATCGGAACCTTCGGTCTGCGTCAGTTAAAAACATTCCTATTCCTGGTGTTCGCTTAGTAGAAGATATTGATGATAATGGAACAGAAATAGCATTTCTTCGCAGTTCTACCAAGTACTTTCAGCAGATGAAAACAGATGTTGAGATGGCTTTAGATCCATCTCGTCTCTTGTATGACATGGATAGTGATGATGAGCGATGGATTTTGAAGTTTCAAAATTCTTCAGAAGTTGAAAAGAGTAGCTCAATGGAGATTGGCGAGGAGATGTTTGAGAAGACAATGGACATGTTTGAGAAGGCTGCATATGTTCGGCAATGTGATCAGTTCACATCTGAAGAAATAGAAGAGTTCATGACTGGTATTGGGCCCATGGATTTGATTAAAACCATTTATGAGCATTGGCGTCAGAAACGGCTGAGAAAGGGAATGCCTTTAATCCGACATCTCCAGCCACCGTCATGGGAGACGTATCAAAAACAAGTGAGGGAGTGGGAGCAAGCTATGACCAAGATGAATAACACACTCGCTAATGGTGGCCGTGAGAAAGCTGCAGCAGTTGAGAAGCCACCTATGTATGCTTTTTGTCTGAAACCACGGGGCCTGGAAGTTCCCAATAAGGGATCAAAGCAACGGTCACAGAAGAAATTTTCTATTTCTGCACATACCAATGCGGTGTTAGGAGATCAGGATGGTTTCCATCCTATTGGAAGGAGATCAAATGGGTTTGCCTATGGTGATGAAAAAATTGCATATTCAGGCCATAACTATGAATCTTTAGATGACTCTCCATTGTCTCAGACGTCGCCCAGGGTTTTTTCACCAAGGGATGCAGCAAACATCTTTATGGGCAATGATGGTTTTGAGAGGAATCATCTTCACAGAATTGACAGAAGCAAGTCAAAGAAATATCGGACAATAGCATCCTCTGTTGACCCCCAGATGGTGTCTCCATATAATCATAGAGTGGTACGAAACAGAAATGGAGTTCATAGAGGGAATATTGGTTTCCCGGAGTGGTCAAGCCAGTCATATTATCAACCAGATGTTGCTCAGAGGCTTGTAAATGCGCATGGCTTTGATCATGATGAGTTTAGGCTTCGTGAGGCATCCGGTGCCGCTCAGCATGCACATAACTTAGCCAAGCGTAAGAGAGAAAATGCTCGCAGATTGTTTTACAGAGCGGATCTAGCAATGCACAAGGCTGTGGTTGCTCTCATGACTGCGGAAGCAATCAAAGCTTCTTCAGAGGACTACGACTATGACTACGACTCTGAGTCCGACTCCTTCTCCATCTCCGACTCTGACACCGATTCAGACAGTGAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPL1

Protein Analysis

1646

Amino Acids

186.25

Weight (kDa)

9.15

Isoelectric Point (pI)

58.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPL1 PF10513 1208 - 1303 1.7e-08 Enhancer of polycomb-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 4415
Acc16I TGCGCA 2 cut(s) 1709, 4680
AccB1I GGYRCC 2 cut(s) 1053, 4724
AccBSI CCGCTC 3 cut(s) 1243, 4730, 4793
AccII CGCG 2 cut(s) 1444, 2781
AccIII TCCGGA 1 cut(s) 274
AciI CCGC 9 cut(s) 1243, 2123, 2706, 2781, 3508, 4283, 4728, 4793, 4835
AclWI GGATC 9 cut(s) 69, 266, 833, 970, 1619, 3557, 3761, 4240, 4803
AcoI YGGCCR 3 cut(s) 1856, 2039, 4147
AcsI RAATTY 7 cut(s) 36, 1928, 2222, 2290, 2913, 3829, 4256
AcuI CTGAAG 6 cut(s) 137, 1290, 1344, 3819, 3966, 4839
AcvI CACGTG 1 cut(s) 3395
AcyI GRCGYC 3 cut(s) 1054, 4015, 4412
AfiI CCNNNNNNNGG 9 cut(s) 1014, 1581, 2727, 2844, 3206, 3589, 3656, 4229, 4552
AflII CTTAAG 3 cut(s) 65, 493, 2618
AflIII ACRYGT 3 cut(s) 942, 3394, 3897
AhdI GACNNNNNGTC 2 cut(s) 1378, 4885
AhlI ACTAGT 3 cut(s) 209, 461, 2876
AjuI GAANNNNNNNTTGG 2 cut(s) 1059, 1091
Alw21I GWGCWC 2 cut(s) 421, 3330
AlwI GGATC 9 cut(s) 69, 266, 833, 970, 1619, 3557, 3761, 4240, 4803
AlwNI CAGNNNCTG 1 cut(s) 115
Ama87I CYCGRG 3 cut(s) 373, 2496, 2684
Aor13HI TCCGGA 1 cut(s) 274
ApaI GGGCCC 2 cut(s) 3583, 3981
ApoI RAATTY 7 cut(s) 36, 1928, 2222, 2290, 2913, 3829, 4256
ArsI GACNNNNNNTTYG 4 cut(s) 887, 919, 4229, 4261
AseI ATTAAT 1 cut(s) 2607
Asp700I GAANNNNTTC 1 cut(s) 618
AspLEI GCGC 4 cut(s) 1056, 1710, 2781, 4681
AsuC2I CCSGG 2 cut(s) 673, 4622
AsuHPI GGTGA 8 cut(s) 512, 696, 1181, 2041, 2153, 2403, 4358, 4422
AvaI CYCGRG 3 cut(s) 373, 2496, 2684
AvaII GGWCC 4 cut(s) 1135, 2463, 2730, 3161
BaeGI GKGCMC 4 cut(s) 2968, 3394, 3583, 3981
BalI TGGCCA 2 cut(s) 1858, 2041
BanI GGYRCC 2 cut(s) 1053, 4724
BanII GRGCYC 3 cut(s) 3330, 3583, 3981
BarI GAAGNNNNNNTAC 2 cut(s) 3452, 3484
BbrPI CACGTG 1 cut(s) 3395
BbsI GAAGAC 3 cut(s) 789, 1114, 3893
Bbv12I GWGCWC 2 cut(s) 421, 3330
BbvCI CCTCAGC 2 cut(s) 522, 1001
BceAI ACGGC 2 cut(s) 4040, 4134
BcgI CGANNNNNNTGC 2 cut(s) 270, 304
BciVI GTATCC 1 cut(s) 1579
BclI TGATCA 3 cut(s) 469, 3931, 4690
BcnI CCSGG 2 cut(s) 673, 4622
BcuI ACTAGT 3 cut(s) 209, 461, 2876
BfaI CTAG 7 cut(s) 210, 462, 1578, 2309, 2844, 2877, 4799
BfmI CTRYAG 3 cut(s) 2976, 2994, 4161
BfoI RGCGCY 1 cut(s) 1057
BfrI CTTAAG 3 cut(s) 65, 493, 2618
BfuI GTATCC 1 cut(s) 1579
BglI GCCNNNNNGGC 1 cut(s) 533
BglII AGATCT 1 cut(s) 53
BlpI GCTNAGC 3 cut(s) 420, 735, 4731
BmcAI AGTACT 2 cut(s) 514, 3728
Bme18I GGWCC 4 cut(s) 1135, 2463, 2730, 3161
BmeRI GACNNNNNGTC 2 cut(s) 1378, 4885
BmeT110I CYCGRG 3 cut(s) 373, 2496, 2684
BmrI ACTGGG 2 cut(s) 861, 3027
BmuI ACTGGG 2 cut(s) 861, 3027
BpiI GAAGAC 3 cut(s) 789, 1114, 3893
BplI GAGNNNNNCTC 4 cut(s) 1450, 1482, 4877, 4909
BpmI CTGGAG 3 cut(s) 1635, 2853, 4043
Bpu10I CCTNAGC 2 cut(s) 522, 1001
Bpu1102I GCTNAGC 3 cut(s) 420, 735, 4731
BpuEI CTTGAG 4 cut(s) 179, 501, 644, 3165
BpuMI CCSGG 2 cut(s) 673, 4622
BsaAI YACGTR 1 cut(s) 3395
BsaBI GATNNNNATC 2 cut(s) 474, 967
BsaHI GRCGYC 3 cut(s) 1054, 4015, 4412
BsaWI WCCGGW 2 cut(s) 274, 4721
Bsc4I CCNNNNNNNGG 9 cut(s) 1014, 1581, 2727, 2844, 3206, 3589, 3656, 4229, 4552
Bse118I RCCGGY 1 cut(s) 449
Bse1I ACTGG 9 cut(s) 856, 1504, 1720, 2194, 2860, 3033, 3574, 3973, 4636
Bse3DI GCAATG 5 cut(s) 2010, 3194, 3932, 4468, 4809
Bse8I GATNNNNATC 2 cut(s) 474, 967
BseAI TCCGGA 1 cut(s) 274
BseJI GATNNNNATC 2 cut(s) 474, 967
BseLI CCNNNNNNNGG 9 cut(s) 1014, 1581, 2727, 2844, 3206, 3589, 3656, 4229, 4552
BseMI GCAATG 5 cut(s) 2010, 3194, 3932, 4468, 4809
BseNI ACTGG 9 cut(s) 856, 1504, 1720, 2194, 2860, 3033, 3574, 3973, 4636
BseRI GAGGAG 5 cut(s) 178, 803, 806, 1814, 3887
BseSI GKGCMC 4 cut(s) 2968, 3394, 3583, 3981
BseYI CCCAGC 1 cut(s) 3038
BsgI GTGCAG 2 cut(s) 2574, 4254
Bsh1236I CGCG 2 cut(s) 1444, 2781
Bsh1285I CGRYCG 1 cut(s) 2785
BshNI GGYRCC 2 cut(s) 1053, 4724
BsiEI CGRYCG 1 cut(s) 2785
BsiHKAI GWGCWC 2 cut(s) 421, 3330
BsiHKCI CYCGRG 3 cut(s) 373, 2496, 2684
BsiSI CCGG 7 cut(s) 275, 450, 673, 1611, 1823, 4622, 4722
BslFI GGGAC 6 cut(s) 164, 655, 1094, 1823, 3021, 3466
BslI CCNNNNNNNGG 9 cut(s) 1014, 1581, 2727, 2844, 3206, 3589, 3656, 4229, 4552
BsmBI CGTCTC 2 cut(s) 3782, 4070
BsmFI GGGAC 6 cut(s) 164, 655, 1094, 1823, 3021, 3466
BsmI GAATGC 8 cut(s) 281, 363, 566, 595, 814, 1948, 2369, 4044
BsoBI CYCGRG 3 cut(s) 373, 2496, 2684
Bsp120I GGGCCC 2 cut(s) 3579, 3977
Bsp1286I GDGCHC 6 cut(s) 421, 2968, 3330, 3394, 3583, 3981
Bsp13I TCCGGA 1 cut(s) 274
Bsp1407I TGTACA 1 cut(s) 75
Bsp1720I GCTNAGC 3 cut(s) 420, 735, 4731
Bsp19I CCATGG 1 cut(s) 3981
Bsp68I TCGCGA 1 cut(s) 1444
BspACI CCGC 9 cut(s) 1243, 2123, 2706, 2781, 3508, 4283, 4728, 4793, 4835
BspEI TCCGGA 1 cut(s) 274
BspFNI CGCG 2 cut(s) 1444, 2781
BspHI TCATGA 3 cut(s) 3963, 4693, 4827
BspMAI CTGCAG 1 cut(s) 4165
BspPI GGATC 9 cut(s) 69, 266, 833, 970, 1619, 3557, 3761, 4240, 4803
BspQI GCTCTTC 1 cut(s) 1812
BspT107I GGYRCC 2 cut(s) 1053, 4724
BspTI CTTAAG 3 cut(s) 65, 493, 2618
BsrBI CCGCTC 3 cut(s) 1243, 4730, 4793
BsrDI GCAATG 5 cut(s) 2010, 3194, 3932, 4468, 4809
BsrFI RCCGGY 1 cut(s) 449
BsrGI TGTACA 1 cut(s) 75
BsrI ACTGG 9 cut(s) 856, 1504, 1720, 2194, 2860, 3033, 3574, 3973, 4636
BssAI RCCGGY 1 cut(s) 449
BssNI GRCGYC 3 cut(s) 1054, 4015, 4412
BssT1I CCWWGG 5 cut(s) 726, 870, 1904, 3981, 4433
BstACI GRCGYC 3 cut(s) 1054, 4015, 4412
BstAFI CTTAAG 3 cut(s) 65, 493, 2618
BstAPI GCANNNNNTGC 1 cut(s) 4724
BstAUI TGTACA 1 cut(s) 75
BstBAI YACGTR 1 cut(s) 3395
BstDSI CCRYGG 2 cut(s) 3981, 4205
BstEII GGTNACC 1 cut(s) 2029
BstENI CCTNNNNNAGG 4 cut(s) 1579, 2842, 3204, 3587
BstFNI CGCG 2 cut(s) 1444, 2781
BstH2I RGCGCY 1 cut(s) 1057
BstHHI GCGC 4 cut(s) 1056, 1710, 2781, 4681
BstMCI CGRYCG 1 cut(s) 2785
BstNSI RCATGY 6 cut(s) 605, 768, 946, 1754, 3901, 4739
BstPI GGTNACC 1 cut(s) 2029
BstSFI CTRYAG 3 cut(s) 2976, 2994, 4161
BstSLI GKGCMC 4 cut(s) 2968, 3394, 3583, 3981
BstUI CGCG 2 cut(s) 1444, 2781
BstV2I GAAGAC 3 cut(s) 789, 1114, 3893
BstX2I RGATCY 5 cut(s) 53, 61, 3549, 3766, 4795
BstXI CCANNNNNNTGG 2 cut(s) 4071, 4316
BstYI RGATCY 5 cut(s) 53, 61, 3549, 3766, 4795
BsuI GTATCC 1 cut(s) 1579
BtgI CCRYGG 2 cut(s) 3981, 4205
BtgZI GCGATG 2 cut(s) 896, 3823
BtsI GCAGTG 2 cut(s) 1943, 3045
BtsIMutI CAGTG 8 cut(s) 30, 1543, 1943, 2644, 2853, 3045, 3581, 4936
BtuMI TCGCGA 1 cut(s) 1444
CaiI CAGNNNCTG 1 cut(s) 115
CciI TCATGA 3 cut(s) 3963, 4693, 4827
CfoI GCGC 4 cut(s) 1056, 1710, 2781, 4681
Cfr10I RCCGGY 1 cut(s) 449
CseI GACGC 3 cut(s) 287, 3621, 4004
DinI GGCGCC 1 cut(s) 1055
DraI TTTAAA 2 cut(s) 1933, 2221
DriI GACNNNNNGTC 2 cut(s) 1378, 4885
EaeI YGGCCR 3 cut(s) 1856, 2039, 4147
Eam1105I GACNNNNNGTC 2 cut(s) 1378, 4885
Ecl136II GAGCTC 1 cut(s) 3328
Eco130I CCWWGG 5 cut(s) 726, 870, 1904, 3981, 4433
Eco147I AGGCCT 1 cut(s) 527
Eco24I GRGCYC 3 cut(s) 3330, 3583, 3981
Eco47I GGWCC 4 cut(s) 1135, 2463, 2730, 3161
Eco53kI GAGCTC 1 cut(s) 3328
Eco57I CTGAAG 6 cut(s) 137, 1290, 1344, 3819, 3966, 4839
Eco72I CACGTG 1 cut(s) 3395
Eco88I CYCGRG 3 cut(s) 373, 2496, 2684
Eco91I GGTNACC 1 cut(s) 2029
EcoICRI GAGCTC 1 cut(s) 3328
EcoNI CCTNNNNNAGG 4 cut(s) 1579, 2842, 3204, 3587
EcoO109I RGGNCCY 3 cut(s) 2730, 3580, 4210
EcoO65I GGTNACC 1 cut(s) 2029
EcoT14I CCWWGG 5 cut(s) 726, 870, 1904, 3981, 4433
EcoT22I ATGCAT 2 cut(s) 1764, 3600
EcoT38I GRGCYC 3 cut(s) 3330, 3583, 3981
EgeI GGCGCC 1 cut(s) 1055
EheI GGCGCC 1 cut(s) 1055
ErhI CCWWGG 5 cut(s) 726, 870, 1904, 3981, 4433
Esp3I CGTCTC 2 cut(s) 3782, 4070
FaqI GGGAC 6 cut(s) 164, 655, 1094, 1823, 3021, 3466
FauI CCCGC 1 cut(s) 2713
FauNDI CATATG 1 cut(s) 3916
FbaI TGATCA 3 cut(s) 469, 3931, 4690
FriOI GRGCYC 3 cut(s) 3330, 3583, 3981
FspAI RTGCGCAY 1 cut(s) 4680
FspBI CTAG 7 cut(s) 210, 462, 1578, 2309, 2844, 2877, 4799
FspI TGCGCA 2 cut(s) 1709, 4680
GlaI GCGC 4 cut(s) 1055, 1709, 2780, 4680
GsaI CCCAGC 1 cut(s) 3042
GsuI CTGGAG 3 cut(s) 1635, 2853, 4043
HaeII RGCGCY 1 cut(s) 1057
HapII CCGG 7 cut(s) 275, 450, 673, 1611, 1823, 4622, 4722
HgaI GACGC 3 cut(s) 287, 3621, 4004
HhaI GCGC 4 cut(s) 1056, 1710, 2781, 4681
Hin1I GRCGYC 3 cut(s) 1054, 4015, 4412
Hin6I GCGC 4 cut(s) 1054, 1708, 2779, 4679
HinP1I GCGC 4 cut(s) 1054, 1708, 2779, 4679
HincII GTYRAC 3 cut(s) 1896, 3501, 4543
HindII GTYRAC 3 cut(s) 1896, 3501, 4543
HindIII AAGCTT 6 cut(s) 237, 341, 2180, 2558, 3431, 4848
HpaI GTTAAC 1 cut(s) 1896
HpaII CCGG 7 cut(s) 275, 450, 673, 1611, 1823, 4622, 4722
HphI GGTGA 8 cut(s) 512, 696, 1181, 2041, 2153, 2403, 4358, 4422
Hpy166II GTNNAC 8 cut(s) 266, 1896, 2336, 2453, 3241, 3501, 3939, 4543
Hpy8I GTNNAC 8 cut(s) 266, 1896, 2336, 2453, 3241, 3501, 3939, 4543
Hpy99I CGWCG 5 cut(s) 143, 146, 687, 1458, 4417
HpyCH4IV ACGT 3 cut(s) 3394, 4079, 4412
HpySE526I ACGT 3 cut(s) 3394, 4079, 4412
Hsp92I GRCGYC 3 cut(s) 1054, 4015, 4412
HspAI GCGC 4 cut(s) 1054, 1708, 2779, 4679
KasI GGCGCC 1 cut(s) 1053
Kpn2I TCCGGA 1 cut(s) 274
Ksp22I TGATCA 3 cut(s) 469, 3931, 4690
KspAI GTTAAC 1 cut(s) 1896
LguI GCTCTTC 1 cut(s) 1812
LmnI GCTCC 8 cut(s) 1458, 1654, 2707, 2834, 3055, 3325, 3570, 4105
MaeI CTAG 7 cut(s) 210, 462, 1578, 2309, 2844, 2877, 4799
MaeII ACGT 3 cut(s) 3394, 4079, 4412
MbiI CCGCTC 3 cut(s) 1243, 4730, 4793
MflI RGATCY 5 cut(s) 53, 61, 3549, 3766, 4795
MhlI GDGCHC 6 cut(s) 421, 2968, 3330, 3394, 3583, 3981
MlsI TGGCCA 2 cut(s) 1858, 2041
MluNI TGGCCA 2 cut(s) 1858, 2041
Mly113I GGCGCC 1 cut(s) 1054
MlyI GAGTC 7 cut(s) 452, 1367, 4388, 4874, 4886, 4895, 4904
MmeI TCCRAC 8 cut(s) 234, 665, 1362, 1436, 2062, 4075, 4914, 4932
Mox20I TGGCCA 2 cut(s) 1858, 2041
Mph1103I ATGCAT 2 cut(s) 1764, 3600
MroI TCCGGA 1 cut(s) 274
MroXI GAANNNNTTC 1 cut(s) 618
MscI TGGCCA 2 cut(s) 1858, 2041
MslI CAYNNNNRTG 7 cut(s) 1062, 2166, 2541, 4069, 4278, 4377, 4454
Msp20I TGGCCA 2 cut(s) 1858, 2041
MspA1I CMGCKG 3 cut(s) 424, 1407, 2588
MspCI CTTAAG 3 cut(s) 65, 493, 2618
MspI CCGG 7 cut(s) 275, 450, 673, 1611, 1823, 4622, 4722
Mva1269I GAATGC 8 cut(s) 281, 363, 566, 595, 814, 1948, 2369, 4044
MvnI CGCG 2 cut(s) 1444, 2781
NarI GGCGCC 1 cut(s) 1054
NciI CCSGG 2 cut(s) 673, 4622
NcoI CCATGG 1 cut(s) 3981
NdeI CATATG 1 cut(s) 3916
NmuCI GTSAC 6 cut(s) 702, 938, 2029, 2409, 3365, 4245
NruI TCGCGA 1 cut(s) 1444
NsbI TGCGCA 2 cut(s) 1709, 4680
NsiI ATGCAT 2 cut(s) 1764, 3600
NspI RCATGY 6 cut(s) 605, 768, 946, 1754, 3901, 4739
PaeI GCATGC 3 cut(s) 768, 1754, 4739
PagI TCATGA 3 cut(s) 3963, 4693, 4827
PasI CCCWGGG 2 cut(s) 1008, 4419
PceI AGGCCT 1 cut(s) 527
PciI ACATGT 2 cut(s) 942, 3897
PciSI GCTCTTC 1 cut(s) 1812
PctI GAATGC 8 cut(s) 281, 363, 566, 595, 814, 1948, 2369, 4044
PdmI GAANNNNTTC 1 cut(s) 618
PfoI TCCNGGA 3 cut(s) 83, 671, 4620
PleI GAGTC 7 cut(s) 451, 1367, 4388, 4874, 4886, 4894, 4904
PluTI GGCGCC 1 cut(s) 1057
PmaCI CACGTG 1 cut(s) 3395
PmlI CACGTG 1 cut(s) 3395
PpsI GAGTC 7 cut(s) 451, 1367, 4388, 4874, 4886, 4894, 4904
Ppu21I YACGTR 1 cut(s) 3395
PpuMI RGGWCCY 1 cut(s) 2730
PscI ACATGT 2 cut(s) 942, 3897
PshBI ATTAAT 1 cut(s) 2607
Psp124BI GAGCTC 1 cut(s) 3330
Psp5II RGGWCCY 1 cut(s) 2730
PspCI CACGTG 1 cut(s) 3395
PspEI GGTNACC 1 cut(s) 2029
PspFI CCCAGC 1 cut(s) 3038
PspOMI GGGCCC 2 cut(s) 3579, 3977
PspPPI RGGWCCY 1 cut(s) 2730
PstI CTGCAG 1 cut(s) 4165
PstNI CAGNNNCTG 1 cut(s) 115
PsuI RGATCY 5 cut(s) 53, 61, 3549, 3766, 4795
PvuII CAGCTG 3 cut(s) 424, 1407, 2588
RruI TCGCGA 1 cut(s) 1444
RseI CAYNNNNRTG 7 cut(s) 1062, 2166, 2541, 4069, 4278, 4377, 4454
SacI GAGCTC 1 cut(s) 3330
SapI GCTCTTC 1 cut(s) 1812
ScaI AGTACT 2 cut(s) 514, 3728
SchI GAGTC 7 cut(s) 452, 1367, 4388, 4874, 4886, 4895, 4904
SduI GDGCHC 6 cut(s) 421, 2968, 3330, 3394, 3583, 3981
SfcI CTRYAG 3 cut(s) 2976, 2994, 4161
SfiI GGCCNNNNNGGCC 1 cut(s) 533
SfoI GGCGCC 1 cut(s) 1055
SinI GGWCC 4 cut(s) 1135, 2463, 2730, 3161
SmiI ATTTAAAT 1 cut(s) 1933
SmiMI CAYNNNNRTG 7 cut(s) 1062, 2166, 2541, 4069, 4278, 4377, 4454
SmlI CTYRAG 7 cut(s) 65, 194, 493, 516, 623, 2618, 3180
SmoI CTYRAG 7 cut(s) 65, 194, 493, 516, 623, 2618, 3180
SpeI ACTAGT 3 cut(s) 209, 461, 2876
SphI GCATGC 3 cut(s) 768, 1754, 4739
SseBI AGGCCT 1 cut(s) 527
SsiI CCGC 9 cut(s) 1243, 2123, 2706, 2781, 3508, 4283, 4728, 4793, 4835
SspDI GGCGCC 1 cut(s) 1053
SspI AATATT 1 cut(s) 4612
SspMI CTAG 7 cut(s) 210, 462, 1578, 2309, 2844, 2877, 4799
SstI GAGCTC 1 cut(s) 3330
StuI AGGCCT 1 cut(s) 527
StyI CCWWGG 5 cut(s) 726, 870, 1904, 3981, 4433
SwaI ATTTAAAT 1 cut(s) 1933
TaiI ACGT 3 cut(s) 3397, 4082, 4415
TaqI TCGA 4 cut(s) 50, 830, 947, 1376
TaqII GACCGA 2 cut(s) 3178, 3615
TatI WGTACW 8 cut(s) 75, 512, 848, 1723, 1778, 2735, 3059, 3726
TauI GCSGC 1 cut(s) 4730
TscAI CASTG 8 cut(s) 37, 1543, 1950, 2644, 2860, 3052, 3581, 4936
TseFI GTSAC 6 cut(s) 702, 938, 2029, 2409, 3365, 4245
Tsp45I GTSAC 6 cut(s) 702, 938, 2029, 2409, 3365, 4245
TspGWI ACGGA 3 cut(s) 625, 1507, 2470
TspRI CASTG 8 cut(s) 37, 1543, 1950, 2644, 2860, 3052, 3581, 4936
Vha464I CTTAAG 3 cut(s) 65, 493, 2618
VpaK11BI GGWCC 4 cut(s) 1135, 2463, 2730, 3161
VspI ATTAAT 1 cut(s) 2607
XagI CCTNNNNNAGG 4 cut(s) 1579, 2842, 3204, 3587
XapI RAATTY 7 cut(s) 36, 1928, 2222, 2290, 2913, 3829, 4256
XbaI TCTAGA 1 cut(s) 1577
XceI RCATGY 6 cut(s) 605, 768, 946, 1754, 3901, 4739
XcmI CCANNNNNNNNNTGG 1 cut(s) 3149
XmnI GAANNNNTTC 1 cut(s) 618
XspI CTAG 7 cut(s) 210, 462, 1578, 2309, 2844, 2877, 4799
ZraI GACGTC 1 cut(s) 4413
ZrmI AGTACT 2 cut(s) 514, 3728
Zsp2I ATGCAT 2 cut(s) 1764, 3600
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.