MD15G1114600.v1.1

Enhancer of polycomb-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010036
Physical Location & Seq
Reverse (-)
8081183 .. 8087821
6639 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1114600.v1.1.491

Sequence Viewer

Length: 4989 bp
ATGGAAAATAGAGTAGAGAACTCACATGGCACTGAAATTCCTGAAAAATCGAGATCTCTGGATCTTAAAACTTTGTATAAATCAAGAAGTAGGAAAGGTGTCGAAAACAAGAGCTTGAAGAGAAAAGTTAGTGCCAAGGATGGCGATGAGAACGGGGGCAAGACGAAGAAGAGTAAGAAAGAGGCTTCTCTTAGTAGTTTGAAGAATGTTAGTACCAGTAGCAAGAAGAGTTTAGATAAAGTGTATCATAGCGGGTTGAGTTCTGGTTCACATGATCCGGAGGCATTGAAATCTGGGTTGAGCGAGAGATTGGATAGCAGTAGTGGGTTGAATGGTGTTTCGTCGCTTAGTTTGAATAATAAGGTCATTCAAATCCCGAGGCGTAAACGGGGTTTTGTAGGGAGGAAGAAATTTGATGGTGGTCACGCTCACAAGCTGCCAGATGAGTCTGCTGGTAAAGTGGGTGTCGTTGATCAGACAAATCAGACAGCTACGTTAAATGTTGATGATTTAGGTGTTCAAGCAGAGTCCTTGAAGGTTAAACGAAAAAAGGGTCTTCATGATTTTAAAGAAAACATAAATAGTGAGTTGAATTCAGCACCACATGCTAAGAAAGAAGACGTTCCTACAAGTCAGTCGGCTGTAAGCAATGGGGATTCATCTTTGAAGAAGTCTCGGAGGAATCGTAGGAAGAGGAAGGATTTGGCATCTCACAGTAAAAGTTCTGATAAGGAAGCTGAGCCTTTGGTAGATGGTTCTACAGAAAAAGGCCATGATTTACAAGAAGATGATGAGGAGAATCTTGAACAGAATGCAGCAAGGATGCTTTCATCAAGGTTTGATCCTAGCTGCACTGGGTTTTCTTCAAACAACAAGGCTTCTGCAAATGGGTTGTCTTTTTTGTTATCTTCTGAACAGGATTTTGGTAGTCATAGGTCAAAGTCTATTTCTGGGTCAGAATCTAATTCGGTTGATAATTCTGGTAGAGTATTGAGGCCGAGGAAACAGCACAATGAAAAGGGACATTCACGGAAGAGGCGCCATTTCTATGAAGTTTTCTTTGGGAACTTGGATGCTTACTGGGTAGTGAACCAGAGAATCAAAGTCTTCTGGCCTCTAGACCAAAGTTGGTACTATGGTCTTGTGAATGACTATGACAAGGAAAAAAAACTTCATCATGTGAAGTACGATGATCGAGACGAAGAATGGATTGACCTTCAAAATGAGAGGTTCAAGCTCTTGTTACTCCCTAGTGAAGTTCCCGGTAAGACAGAGCAGAAAAAATCAAAGGTGAGGAATAGAAGTCCTGATGAGAGAAAAGGAGACATGAAATGCAGAAAAGAAAAGAAAAAGAGAGACTTGACTTCAGAGGACGGTAGCCGCATAGGCAGCTATATGGACACTGAACCCATTATTTCATGGTTGGCGCGATCTACGGGTCGGGTCAAGTCATCCTCCTGTGCTGTGAAGGAGCAAAAAACATCTGGTCTATCTTTAAAGTCTGTGCCACCATTGTCAGATGAAGATGCCACTTTGCATGAATCTTTGGGTGATGGCTCCTTTAGAAGAGATAAAAATAAAAAAATTTCTCGCCATCCTGGCAGATCATCTGATGATGTGAGGCAAGAAAAGTCTACCTCTCAGGGCAGCACTGGCCTCAAAGATAGCAAAATGCCTATTGTTTACTTTCGAAGGCGGCTTCGGAAGAATGAGTCTGAATTGTCTCATACATCCGAGGATGACCATGCCTCTGTGAGTAAGCTTGGGTCACTTTATGATTTCTTGGGAAGCTTGGATGTCAATGGACCATTGTGGTCTATTGATGATGCAGGACGGCTGAAATTAACTCCTCCACGGATAGAACCAGGAAGAGTTACTTTTGAGTTAGGCCTTCCAGTGCATTCAATCACTAATGATTCTTTTGGAGTAGAGTTTTGGTTGTTTCGTGCTGCAATGCTGTGTAGGTATGGTGCAGTGGTGATTTCATGGCCAAAGGTTTACTTGGAGATGCTTTTTGTTGACAATGTAGTTGGAGTGAGATTTCTGTTGTTTGAAGGTTGCTTGAAGCAGGCTGTGTCCTTTGTTTTTCTTGTCCTGTCATTGTTTCATCAACCCAATGACCAGGGGAAGTCTGTTGATTTCCAGTTACCAGCAACTTCAATCAGGTTCAAATTCTCCTGTGTTCAGCATCTTGGGAAGCAACTTGTGTTTGCATTCTACAACTTTTTGGAAGTAAAAAATTCAAAGTGGAAGTACCTAGACTCTCAACTTATGAGCCATTGTTTGCTTACCAAGAAACTGCCCCCGTCTGAATGCACTTATGATAGTATTCAAGCACTTCAAAATGGAAGAAATCAATCACCTTTTATGTCTCTTTGTGCGCATTCCTCCTTTGTCAAGGGTACACAGAGGAGGTCCAGGCAGGGTATTAACTTCATGGGTGGTTCCAGGGAATCTACATCTGTAAATATTAGTCATCCTACTTCTCGTAATGATGCGCTATGCCGAAAACTTCCTCCACTTGCCCTCTCTTTTGCTGCTGCTCCTACTTTCTTCATTAGTTTGCATCTTAAACTGCTCATGGAAAATTGTGTTGCTAATATCTGCTTTGGCGACCGTGATTCAGTGGAGCATGTCGAAAACTCCGGCAGTATGTTGGCAGTTGACTGGTCTATTGTTGAGGACTTTATTAGCGAAGGTTCGAAAATTACTCCTCAGAAAAATTTGAAGGCTCCACCAAGTGATGCTACTTCTGATGGGTCCTGTGCCAAACCAGATGCAGAAAATTCTATTTCTGTATGCCATGGAGCTCGGACAAATTCATCTCAGCATTTCCAAAATGGTGGTCTTTATGTTTCTGTATCCTCTGGTGGAACTGGTGTCCTTGAAAAGACTGGAACAGATGAAGTTGTACAGTCGAAGGTGTTGCAAAGCCATCATCCAGAGTCAGATCAATGTTCTTTATCCCCAAGGCCTTTGGTTGGTAGAGACAAGTCCGACACTGATTCCCAATCTTTTCCGAACGGTCTCACTGTTGAAATTCCATCATTTGACCGATTTGAGAAGCCTGTGGATAAGGAGGTGCAAAGTGCTCAACAGCCTACAGACTTTTCTTGGAATATGAATGGTAGCATTATCCCCAGCCCTAACCCCACTGCTCCCAGAAGTACAGGGCACCGAAATAGAAATAATTCATCACTTGGACACCTCTCACATAATTGGTCTGATGGAACTGATCTTTTCCATAATGGTTTTGGCAGTGGACCAAAGAAGCCGCGAACTCAGGTGTCCTACACTTTGCCTTATGGAGGTTTTGATTTTAGTTCGAAGCAAAGAAACCTTCAGAAGGGGCTTCCTCATAAACGAATCAGGAGGGCTAATAATGAGAAGAGGTCATCAGATGCTTCCAGAGGTTCCCAAAGAAACTTGGAGCTCTTATCATGCGAGGCAAATGTGTTAGTCAATGGCAGTGACAGAGGGTGGAGGGAATGTGGGGCCCATGTTGTATTAGAGCTTTTTGATCATAATGAGTGGAAGCTTGCTGTAAAAATTTCAGGGACTACAAAGTACTCATACAAAGCACATCAATTTTTGCAGCCTGGGACAACAAACCGCTATACTCATGCTATGATGTGGAAAGGAGGAAAGGATTGGAATTGGGGTTTAGAGTTTCCAGATAGGAGTCAGTGGGCACTTTTTAGGGAGATGCATGAAGAATGTTACAATCGGAATATCCGCTCTGCTTCAGTAAAAAATATTCCTATTCCTGGTGTCCGCTTAATTGAAGAAAGTGATGATAATTTAACTGAAATATCATTTCTTCGCAGCTCTGCAAAGTATTTCCGGCAAATAGAAACAGATGTTGAAATGGCATTGGACCCATCTCGGGTCTTGTATGATATGGATAGTGATGATGAGCAGTGGATTTTGAAGTTTCAAAATTCTTCAGAAGTTCACAATTGTGGCTCAACAGAGATCGAAGATGAGATGTTTGAGAAAACAATGGACATGTTTGAAAAGGCTGCATTTGATCAACAGTGTGATGAGTTCACGTCAGAAGAAATAGAAGAGCTTGTGGATGGTGCTGGAGTTGGGCCCATGGACGTGATCTTATCCATTTATGAGCATTGGCAACAGAAAAGGCTGAGGAAGGGAATGCCTTTAATCCGACATCTTCAGCCACCATCATGGGAAAGGTATCAACAACAAGTGAAAGAGTGGGAGCAAGCTATGATCAAAACCAATACCACGCTCCCCAATGGATGCCATTGGAAACCTGCATCAGTTGAAAAGCCACCAATGTTTGCTTTTTGTCTGAAACCACGGGGACTAGAAGTTCCCAACAAGGGATCTAAACAACGGTCACAGAGGAAATTTTCAGTTTCTGGACATAATGGTGTCATGTTAGGAGATCACGATGGTTTCCATGCTTTTGGAAGGAGATCAAATGGGTTTGCTTTCGGTGATGAAAGGGTGGTATATCCAGGCCATAACTATGACTCTTTAGAAGACTCCCCCTTGTCTCAGACATCGCCTGGGGTATTTTCACCAAGGGATGCAGCTAACATGTTGATGAGCAATGATGGGTTTGAGAGAAATCATCTCCGCAGAATTCATAGGAGCAAGTCAAAGAAATATGGGAGGACGGTATCTTCTGTTGGCCCCAGTCGTAGAGTCGTAGGAAACAGAAATGAAGTTCATCGATGGAATGCAGGTATCCCTGATTGGTCAAGCCAGCGGTATTATCAACCAGAGGTGTCTCCGAGGCACGGCATGGGACTGCTGGACGATTCTGATCTCGATGAGTTCAGATTGCGTGATGCATCTGGAGCCGCCCAGCATGCACATAAAATGGCAAGGATCAAGAGAGATAAAGCTCAGAGATTGTTTTACAGAGCAGATCTAGCAATCCACAGGGCCGTGGTTTCTCTCATGACCGCGGAAGCGATCAAAACTTCTTCCGAGGACTCCAGTGACGATGAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPL1

Protein Analysis

1663

Amino Acids

187.04

Weight (kDa)

9.01

Isoelectric Point (pI)

51.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPL1 PF10513 1245 - 1340 9.8e-11 Enhancer of polycomb-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2382
Acc36I ACCTGC 2 cut(s) 4285, 4703
AccB1I GGYRCC 2 cut(s) 1038, 3174
AccBSI CCGCTC 1 cut(s) 3738
AccI GTMKAC 1 cut(s) 1634
AccII CGCG 3 cut(s) 1429, 3277, 4940
AccIII TCCGGA 1 cut(s) 277
AclWI GGATC 5 cut(s) 69, 269, 836, 4357, 4868
AcoI YGGCCR 1 cut(s) 1988
AcuI CTGAAG 5 cut(s) 1350, 3326, 3729, 3930, 4160
AcyI GRCGYC 1 cut(s) 1039
AdeI CACNNNGTG 1 cut(s) 2741
AfaI GTAC 8 cut(s) 214, 1133, 1187, 2255, 2404, 2913, 3169, 3569
AfiI CCNNNNNNNGG 7 cut(s) 2981, 3308, 3346, 3767, 3886, 4346, 4769
AflIII ACRYGT 2 cut(s) 4008, 4566
AjiI CACGTC 2 cut(s) 4053, 4105
AjnI CCWGG 9 cut(s) 1597, 1864, 2121, 2417, 2447, 3598, 3766, 4483, 4534
AjuI GAANNNNNNNTTGG 4 cut(s) 906, 938, 1044, 1076
AleI CACNNNNGTG 1 cut(s) 3960
AloI GAACNNNNNNTCC 2 cut(s) 4320, 4352
Alw21I GWGCWC 3 cut(s) 2812, 3094, 3435
AlwI GGATC 5 cut(s) 69, 269, 836, 4357, 4868
AlwNI CAGNNNCTG 1 cut(s) 4385
Ama87I CYCGRG 2 cut(s) 376, 3885
Aor13HI TCCGGA 1 cut(s) 277
ApaI GGGCCC 2 cut(s) 3499, 4098
ArsI GACNNNNNNTTYG 6 cut(s) 538, 570, 878, 910, 2830, 2862
Asp700I GAANNNNTTC 2 cut(s) 621, 3190
AspLEI GCGC 4 cut(s) 1041, 1429, 2383, 2500
AsuC2I CCSGG 1 cut(s) 1263
AsuHPI GGTGA 6 cut(s) 1303, 1562, 1990, 2352, 4475, 4539
AsuII TTCGAA 3 cut(s) 1690, 2702, 3326
AvaI CYCGRG 2 cut(s) 376, 3885
AvaII GGWCC 5 cut(s) 1805, 2415, 2760, 3263, 3877
BaeGI GKGCMC 4 cut(s) 3177, 3499, 3694, 4098
BaeI ACNNNNGTAYC 2 cut(s) 1123, 1156
BalI TGGCCA 1 cut(s) 1990
BanI GGYRCC 2 cut(s) 1038, 3174
BanII GRGCYC 4 cut(s) 2812, 3435, 3499, 4098
BbsI GAAGAC 4 cut(s) 548, 624, 1099, 4515
Bbv12I GWGCWC 3 cut(s) 2812, 3094, 3435
BbvCI CCTCAGC 1 cut(s) 4145
BceAI ACGGC 3 cut(s) 1850, 4786, 4904
BcgI CGANNNNNNTGC 2 cut(s) 2908, 2942
BciT130I CCWGG 9 cut(s) 1599, 1866, 2123, 2419, 2449, 3600, 3768, 4485, 4536
BciVI GTATCC 2 cut(s) 2872, 4727
BclI TGATCA 4 cut(s) 472, 3520, 4030, 4233
BcnI CCSGG 1 cut(s) 1263
BfaI CTAG 6 cut(s) 846, 1118, 1251, 2258, 4331, 4904
BfmI CTRYAG 2 cut(s) 759, 3102
BfoI RGCGCY 1 cut(s) 1042
BfuAI ACCTGC 2 cut(s) 4285, 4703
BfuI GTATCC 2 cut(s) 2872, 4727
BglI GCCNNNNNGGC 2 cut(s) 1386, 1599
BglII AGATCT 2 cut(s) 53, 4900
BlpI GCTNAGC 1 cut(s) 738
BmcAI AGTACT 1 cut(s) 3569
Bme18I GGWCC 5 cut(s) 1805, 2415, 2760, 3263, 3877
BmeT110I CYCGRG 2 cut(s) 376, 3885
BmgBI CACGTC 2 cut(s) 4053, 4105
BmrI ACTGGG 3 cut(s) 864, 1090, 4659
BmuI ACTGGG 3 cut(s) 864, 1090, 4659
BpiI GAAGAC 4 cut(s) 548, 624, 1099, 4515
BpmI CTGGAG 3 cut(s) 4107, 4848, 4954
Bpu10I CCTNAGC 1 cut(s) 4145
Bpu1102I GCTNAGC 1 cut(s) 738
Bpu14I TTCGAA 3 cut(s) 1690, 2702, 3326
BpuMI CCSGG 1 cut(s) 1263
Bsa29I ATCGAT 1 cut(s) 4702
BsaBI GATNNNNATC 1 cut(s) 4794
BsaHI GRCGYC 1 cut(s) 1039
BsaI GGTCTC 1 cut(s) 3032
BsaWI WCCGGW 1 cut(s) 277
BsaXI ACNNNNNCTCC 4 cut(s) 670, 700, 3475, 3505
Bsc4I CCNNNNNNNGG 7 cut(s) 2981, 3308, 3346, 3767, 3886, 4346, 4769
Bse3DI GCAATG 3 cut(s) 655, 1959, 4585
Bse8I GATNNNNATC 1 cut(s) 4794
BseAI TCCGGA 1 cut(s) 277
BseBI CCWGG 9 cut(s) 1599, 1866, 2123, 2419, 2449, 3600, 3768, 4485, 4536
BseCI ATCGAT 1 cut(s) 4702
BseJI GATNNNNATC 1 cut(s) 4794
BseLI CCNNNNNNNGG 7 cut(s) 2981, 3308, 3346, 3767, 3886, 4346, 4769
BseMI GCAATG 3 cut(s) 655, 1959, 4585
BseMII CTCAG 8 cut(s) 729, 1655, 2729, 2840, 3296, 4136, 4538, 4892
BseRI GAGGAG 4 cut(s) 809, 1839, 2425, 2703
BseSI GKGCMC 4 cut(s) 3177, 3499, 3694, 4098
BseYI CCCAGC 2 cut(s) 3140, 4836
BsgI GTGCAG 2 cut(s) 835, 1992
Bsh1236I CGCG 3 cut(s) 1429, 3277, 4940
Bsh1285I CGRYCG 1 cut(s) 2617
BshNI GGYRCC 2 cut(s) 1038, 3174
BshVI ATCGAT 1 cut(s) 4702
BsiEI CGRYCG 1 cut(s) 2617
BsiHKAI GWGCWC 3 cut(s) 2812, 3094, 3435
BsiHKCI CYCGRG 2 cut(s) 376, 3885
BsiSI CCGG 4 cut(s) 278, 1263, 2646, 3844
BslFI GGGAC 5 cut(s) 1035, 3571, 3616, 4341, 4791
BslI CCNNNNNNNGG 7 cut(s) 2981, 3308, 3346, 3767, 3886, 4346, 4769
BsmBI CGTCTC 1 cut(s) 1191
BsmFI GGGAC 5 cut(s) 1035, 3571, 3616, 4341, 4791
BsmI GAATGC 7 cut(s) 817, 1900, 2213, 2318, 2383, 4161, 4714
Bso31I GGTCTC 1 cut(s) 3032
BsoBI CYCGRG 2 cut(s) 376, 3885
Bsp119I TTCGAA 3 cut(s) 1690, 2702, 3326
Bsp120I GGGCCC 2 cut(s) 3495, 4094
Bsp1286I GDGCHC 7 cut(s) 2812, 3094, 3177, 3435, 3499, 3694, 4098
Bsp13I TCCGGA 1 cut(s) 277
Bsp1407I TGTACA 1 cut(s) 2911
Bsp1720I GCTNAGC 1 cut(s) 738
Bsp19I CCATGG 2 cut(s) 2803, 4098
BspCNI CTCAG 8 cut(s) 730, 1654, 2728, 2839, 3295, 4137, 4537, 4891
BspDI ATCGAT 1 cut(s) 4702
BspEI TCCGGA 1 cut(s) 277
BspFNI CGCG 3 cut(s) 1429, 3277, 4940
BspHI TCATGA 2 cut(s) 559, 4932
BspMI ACCTGC 2 cut(s) 4285, 4703
BspPI GGATC 5 cut(s) 69, 269, 836, 4357, 4868
BspQI GCTCTTC 1 cut(s) 4062
BspT104I TTCGAA 3 cut(s) 1690, 2702, 3326
BspT107I GGYRCC 2 cut(s) 1038, 3174
BspTNI GGTCTC 1 cut(s) 3032
BsrBI CCGCTC 1 cut(s) 3738
BsrDI GCAATG 3 cut(s) 655, 1959, 4585
BsrGI TGTACA 1 cut(s) 2911
BssNI GRCGYC 1 cut(s) 1039
BssT1I CCWWGG 5 cut(s) 135, 2803, 2969, 4098, 4550
Bst2UI CCWGG 9 cut(s) 1599, 1866, 2123, 2419, 2449, 3600, 3768, 4485, 4536
Bst4CI ACNGT 9 cut(s) 716, 1376, 2618, 2916, 3026, 3034, 4038, 4362, 4648
BstACI GRCGYC 1 cut(s) 1039
BstAPI GCANNNNNTGC 1 cut(s) 605
BstAUI TGTACA 1 cut(s) 2911
BstBI TTCGAA 3 cut(s) 1690, 2702, 3326
BstC8I GCNNGC 5 cut(s) 2070, 3540, 4227, 4736, 4842
BstDSI CCRYGG 6 cut(s) 1853, 2803, 4098, 4322, 4920, 4938
BstENI CCTNNNNNAGG 2 cut(s) 3306, 3344
BstFNI CGCG 3 cut(s) 1429, 3277, 4940
BstH2I RGCGCY 1 cut(s) 1042
BstHHI GCGC 4 cut(s) 1041, 1429, 2383, 2500
BstMCI CGRYCG 1 cut(s) 2617
BstMWI GCNNNNNNNGC 8 cut(s) 605, 1386, 1389, 1599, 1653, 4829, 4841, 4904
BstNI CCWGG 9 cut(s) 1599, 1866, 2123, 2419, 2449, 3600, 3768, 4485, 4536
BstNSI RCATGY 5 cut(s) 608, 2636, 4012, 4570, 4844
BstSFI CTRYAG 2 cut(s) 759, 3102
BstSLI GKGCMC 4 cut(s) 3177, 3499, 3694, 4098
BstUI CGCG 3 cut(s) 1429, 3277, 4940
BstV2I GAAGAC 4 cut(s) 548, 624, 1099, 4515
BstX2I RGATCY 4 cut(s) 53, 61, 4349, 4900
BstXI CCANNNNNNTGG 3 cut(s) 2843, 4188, 4433
BstYI RGATCY 4 cut(s) 53, 61, 4349, 4900
Bsu15I ATCGAT 1 cut(s) 4702
BsuI GTATCC 2 cut(s) 2872, 4727
BsuTUI ATCGAT 1 cut(s) 4702
BtgI CCRYGG 6 cut(s) 1853, 2803, 4098, 4322, 4920, 4938
BtgZI GCGATG 2 cut(s) 159, 4515
BtrI CACGTC 2 cut(s) 4053, 4105
BtsI GCAGTG 5 cut(s) 1980, 3153, 3265, 3475, 3926
BveI ACCTGC 2 cut(s) 4285, 4703
Cac8I GCNNGC 5 cut(s) 2070, 3540, 4227, 4736, 4842
CaiI CAGNNNCTG 1 cut(s) 4385
CciI TCATGA 2 cut(s) 559, 4932
CfoI GCGC 4 cut(s) 1041, 1429, 2383, 2500
Cfr42I CCGCGG 1 cut(s) 4941
ClaI ATCGAT 1 cut(s) 4702
Csp6I GTAC 8 cut(s) 213, 1132, 1186, 2254, 2403, 2912, 3168, 3568
CviQI GTAC 8 cut(s) 213, 1132, 1186, 2254, 2403, 2912, 3168, 3568
DinI GGCGCC 1 cut(s) 1040
DraI TTTAAA 2 cut(s) 568, 1497
DraIII CACNNNGTG 1 cut(s) 2741
EaeI YGGCCR 1 cut(s) 1988
Ecl136II GAGCTC 2 cut(s) 2810, 3433
Eco130I CCWWGG 5 cut(s) 135, 2803, 2969, 4098, 4550
Eco147I AGGCCT 2 cut(s) 1890, 2974
Eco24I GRGCYC 4 cut(s) 2812, 3435, 3499, 4098
Eco31I GGTCTC 1 cut(s) 3032
Eco47I GGWCC 5 cut(s) 1805, 2415, 2760, 3263, 3877
Eco53kI GAGCTC 2 cut(s) 2810, 3433
Eco57I CTGAAG 5 cut(s) 1350, 3326, 3729, 3930, 4160
Eco88I CYCGRG 2 cut(s) 376, 3885
EcoICRI GAGCTC 2 cut(s) 2810, 3433
EcoNI CCTNNNNNAGG 2 cut(s) 3306, 3344
EcoO109I RGGNCCY 2 cut(s) 2760, 3495
EcoRI GAATTC 2 cut(s) 592, 4611
EcoRII CCWGG 9 cut(s) 1597, 1864, 2121, 2417, 2447, 3598, 3766, 4483, 4534
EcoT14I CCWWGG 5 cut(s) 135, 2803, 2969, 4098, 4550
EcoT22I ATGCAT 2 cut(s) 3711, 4825
EcoT38I GRGCYC 4 cut(s) 2812, 3435, 3499, 4098
EgeI GGCGCC 1 cut(s) 1040
EheI GGCGCC 1 cut(s) 1040
ErhI CCWWGG 5 cut(s) 135, 2803, 2969, 4098, 4550
Esp3I CGTCTC 1 cut(s) 1191
FalI AAGNNNNNCTT 6 cut(s) 1343, 1375, 1861, 1893, 1985, 2017
FaqI GGGAC 5 cut(s) 1035, 3571, 3616, 4341, 4791
FauI CCCGC 1 cut(s) 245
FbaI TGATCA 4 cut(s) 472, 3520, 4030, 4233
FblI GTMKAC 1 cut(s) 1634
FriOI GRGCYC 4 cut(s) 2812, 3435, 3499, 4098
FspAI RTGCGCAY 1 cut(s) 2382
FspBI CTAG 6 cut(s) 846, 1118, 1251, 2258, 4331, 4904
FspI TGCGCA 1 cut(s) 2382
GlaI GCGC 4 cut(s) 1040, 1428, 2382, 2499
GsaI CCCAGC 2 cut(s) 3144, 4840
GsuI CTGGAG 3 cut(s) 4107, 4848, 4954
HaeII RGCGCY 1 cut(s) 1042
HapII CCGG 4 cut(s) 278, 1263, 2646, 3844
HhaI GCGC 4 cut(s) 1041, 1429, 2383, 2500
Hin1I GRCGYC 1 cut(s) 1039
Hin6I GCGC 4 cut(s) 1039, 1427, 2381, 2498
HinP1I GCGC 4 cut(s) 1039, 1427, 2381, 2498
HincII GTYRAC 2 cut(s) 2020, 2665
HindII GTYRAC 2 cut(s) 2020, 2665
HindIII AAGCTT 3 cut(s) 1760, 1789, 3536
HpaII CCGG 4 cut(s) 278, 1263, 2646, 3844
HphI GGTGA 6 cut(s) 1303, 1562, 1990, 2352, 4475, 4539
Hpy99I CGWCG 1 cut(s) 346
HpyCH4III ACNGT 9 cut(s) 716, 1376, 2618, 2916, 3026, 3034, 4038, 4362, 4648
HpyCH4IV ACGT 4 cut(s) 494, 621, 4052, 4104
HpyF10VI GCNNNNNNNGC 8 cut(s) 605, 1386, 1389, 1599, 1653, 4829, 4841, 4904
HpySE526I ACGT 4 cut(s) 494, 621, 4052, 4104
Hsp92I GRCGYC 1 cut(s) 1039
HspAI GCGC 4 cut(s) 1039, 1427, 2381, 2498
KasI GGCGCC 1 cut(s) 1038
Kpn2I TCCGGA 1 cut(s) 277
Ksp22I TGATCA 4 cut(s) 472, 3520, 4030, 4233
KspI CCGCGG 1 cut(s) 4941
LguI GCTCTTC 1 cut(s) 4062
MaeI CTAG 6 cut(s) 846, 1118, 1251, 2258, 4331, 4904
MaeII ACGT 4 cut(s) 494, 621, 4052, 4104
MaeIII GTNAC 9 cut(s) 422, 1242, 1767, 1873, 2145, 3470, 3719, 4362, 4973
MbiI CCGCTC 1 cut(s) 3738
MfeI CAATTG 1 cut(s) 3958
MflI RGATCY 4 cut(s) 53, 61, 4349, 4900
MhlI GDGCHC 7 cut(s) 2812, 3094, 3177, 3435, 3499, 3694, 4098
MlsI TGGCCA 1 cut(s) 1990
MluNI TGGCCA 1 cut(s) 1990
Mly113I GGCGCC 1 cut(s) 1039
MmeI TCCRAC 3 cut(s) 2011, 3021, 4192
Mox20I TGGCCA 1 cut(s) 1990
Mph1103I ATGCAT 2 cut(s) 3711, 4825
MroI TCCGGA 1 cut(s) 277
MroXI GAANNNNTTC 2 cut(s) 621, 3190
MscI TGGCCA 1 cut(s) 1990
MslI CAYNNNNRTG 7 cut(s) 1047, 3960, 4103, 4186, 4395, 4494, 4571
Msp20I TGGCCA 1 cut(s) 1990
MspA1I CMGCKG 2 cut(s) 4738, 4940
MspI CCGG 4 cut(s) 278, 1263, 2646, 3844
MunI CAATTG 1 cut(s) 3958
Mva1269I GAATGC 7 cut(s) 817, 1900, 2213, 2318, 2383, 4161, 4714
MvaI CCWGG 9 cut(s) 1599, 1866, 2123, 2419, 2449, 3600, 3768, 4485, 4536
MvnI CGCG 3 cut(s) 1429, 3277, 4940
MwoI GCNNNNNNNGC 8 cut(s) 605, 1386, 1389, 1599, 1653, 4829, 4841, 4904
NarI GGCGCC 1 cut(s) 1039
NciI CCSGG 1 cut(s) 1263
NcoI CCATGG 2 cut(s) 2803, 4098
NmeAIII GCCGAG 1 cut(s) 1023
NmuCI GTSAC 5 cut(s) 422, 1767, 3470, 4362, 4973
NsbI TGCGCA 1 cut(s) 2382
NsiI ATGCAT 2 cut(s) 3711, 4825
NspI RCATGY 5 cut(s) 608, 2636, 4012, 4570, 4844
NspV TTCGAA 3 cut(s) 1690, 2702, 3326
OliI CACNNNNGTG 1 cut(s) 3960
PaeI GCATGC 1 cut(s) 4844
PagI TCATGA 2 cut(s) 559, 4932
PceI AGGCCT 2 cut(s) 1890, 2974
PciI ACATGT 2 cut(s) 4008, 4566
PciSI GCTCTTC 1 cut(s) 4062
PcsI WCGNNNNNNNCGW 1 cut(s) 682
PctI GAATGC 7 cut(s) 817, 1900, 2213, 2318, 2383, 4161, 4714
PdmI GAANNNNTTC 2 cut(s) 621, 3190
PluTI GGCGCC 1 cut(s) 1042
PpuMI RGGWCCY 1 cut(s) 2760
PscI ACATGT 2 cut(s) 4008, 4566
Psp124BI GAGCTC 2 cut(s) 2812, 3435
Psp5II RGGWCCY 1 cut(s) 2760
Psp6I CCWGG 9 cut(s) 1597, 1864, 2121, 2417, 2447, 3598, 3766, 4483, 4534
PspFI CCCAGC 2 cut(s) 3140, 4836
PspGI CCWGG 9 cut(s) 1597, 1864, 2121, 2417, 2447, 3598, 3766, 4483, 4534
PspOMI GGGCCC 2 cut(s) 3495, 4094
PspPPI RGGWCCY 1 cut(s) 2760
PstNI CAGNNNCTG 1 cut(s) 4385
PsuI RGATCY 4 cut(s) 53, 61, 4349, 4900
RsaI GTAC 8 cut(s) 214, 1133, 1187, 2255, 2404, 2913, 3169, 3569
RsaNI GTAC 8 cut(s) 213, 1132, 1186, 2254, 2403, 2912, 3168, 3568
RseI CAYNNNNRTG 7 cut(s) 1047, 3960, 4103, 4186, 4395, 4494, 4571
SacI GAGCTC 2 cut(s) 2812, 3435
SacII CCGCGG 1 cut(s) 4941
SapI GCTCTTC 1 cut(s) 4062
ScaI AGTACT 1 cut(s) 3569
SduI GDGCHC 7 cut(s) 2812, 3094, 3177, 3435, 3499, 3694, 4098
SfcI CTRYAG 2 cut(s) 759, 3102
SfoI GGCGCC 1 cut(s) 1040
Sfr303I CCGCGG 1 cut(s) 4941
SfuI TTCGAA 3 cut(s) 1690, 2702, 3326
SgrBI CCGCGG 1 cut(s) 4941
SinI GGWCC 5 cut(s) 1805, 2415, 2760, 3263, 3877
SmiMI CAYNNNNRTG 7 cut(s) 1047, 3960, 4103, 4186, 4395, 4494, 4571
SphI GCATGC 1 cut(s) 4844
SseBI AGGCCT 2 cut(s) 1890, 2974
SspDI GGCGCC 1 cut(s) 1038
SspI AATATT 2 cut(s) 2470, 3757
SspMI CTAG 6 cut(s) 846, 1118, 1251, 2258, 4331, 4904
SstI GAGCTC 2 cut(s) 2812, 3435
StuI AGGCCT 2 cut(s) 1890, 2974
StyI CCWWGG 5 cut(s) 135, 2803, 2969, 4098, 4550
TaaI ACNGT 9 cut(s) 716, 1376, 2618, 2916, 3026, 3034, 4038, 4362, 4648
TaiI ACGT 4 cut(s) 497, 624, 4055, 4107
TaqII GACCGA 1 cut(s) 3069
TatI WGTACW 3 cut(s) 2911, 3167, 3567
TauI GCSGC 4 cut(s) 1383, 1699, 3277, 4835
TseFI GTSAC 5 cut(s) 422, 1767, 3470, 4362, 4973
Tsp45I GTSAC 5 cut(s) 422, 1767, 3470, 4362, 4973
TspGWI ACGGA 2 cut(s) 1045, 1870
VpaK11BI GGWCC 5 cut(s) 1805, 2415, 2760, 3263, 3877
XagI CCTNNNNNAGG 2 cut(s) 3306, 3344
XbaI TCTAGA 1 cut(s) 1117
XceI RCATGY 5 cut(s) 608, 2636, 4012, 4570, 4844
XcmI CCANNNNNNNNNTGG 1 cut(s) 3251
XmiI GTMKAC 1 cut(s) 1634
XmnI GAANNNNTTC 2 cut(s) 621, 3190
XspI CTAG 6 cut(s) 846, 1118, 1251, 2258, 4331, 4904
ZrmI AGTACT 1 cut(s) 3569
Zsp2I ATGCAT 2 cut(s) 3711, 4825
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.