RLG00000010305

Enhancer of polycomb-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0010036
Physical Location & Seq
Forward (+)
431785 .. 438215
6431 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010305

Sequence Viewer

Length: 5265 bp
ATGGCATACTTTACGGAGAATGATTCTCAGTGTGACTACTGTGCGAACGTAAGCAGCCGAGCAGCAATCCAACATCCCAGATTGCGCCACGTGGCATTATCCAAAGGATTCGCGTATTTTCGATCGAAACCCTTTGTTATTTTGGTGGAAGTGGAACCGAAAAAGAAAACCCTAGCTCTCTCTGGGTTTCGCCTCAGGAGGCCGGATTCGAATCCCAAATCATGCTCTTGCTTCGTGATCTTCATGACTGTTGGAGGAAGCGATGATTCGGCGTGTTACGATCTCAAAAATGTGTGGGGGCATGTAGGACGGAATGTCAAGTTTGGGGCGTCTCGGTTTGAGGAATTGTTGATGGAAAATAGAATAGAGGTTTCACATGGCACTGAAATTCCCAGGAAATCGAGATCTTTGGATCTTAAGAGTTTGTACAAGTCCAGGAGTAGAAAAGAGGCTGAAAATCAGAGCCTGAAGAGAAAAGGGAGTGAGGACGACGACGGTGGGGACAAGAAGAAGAGGAGTAGGAAAGAGGTGTCTCTCAGTAGTCTCAAGAATGTTAATACTAGTAGTAGCAAGAAGAGTATAGATAAAGTTTATCATAGAGGGTTGGATTCTGGTTCACATGATCCGGACGCATTCAAATCGGGGTCAAGTCAGAAATTGGATAGCAGTAGTGGGTTAAATGGCGTTTCAAAGCTTAGTTTGGATAATAGTGGCATTCAAATCCCGAGGCGTAAGAGGGGATTGGTGGGGCGAAAAAAATGTGAGGGTGCTCAGCTGTTGAAGCTGTCAGATGAGTCTGCCGGTAGAGCAACTAGTGTTGATCAGAATCATCAGATAGCTAAGTTAAGTGGTGATGATTTGGGTACTCAAGCTGAGGCCTGGAAGGCCAAAAGAAACAAAGGTTTGGATGAATGCAAAGAAAATATAAATAGTGAGTTGAATGCAACTTCACATGCAAAGAAAGAAAATGTTCTTGAGAGGCAGTCAGTTGTAAGCAATGGTGATTCATCTTTGAAAAAGTCCCGGAGGAATTGTCGGAAAAGGAAGGACTTGTCACCTGATAGTAAAACTGATGCAAAGGACGCTGAGCCTTTAGTCAATAGTTCTACTAAAGCATGCCAGGCTTCACCAGAAGACGAGGAGGAGAATCTTGAAGAGAATGCAGCGAGGATGCTTTCATCGAGGTTTGATCCAAGCTGTACTGGGTTTTCATTAAACACCAAGGCTTCTGCGATGCAATCTTCAAACGGGTTGTCTAATCAGTCATCTCCTGGTCAGGATTTTGATAGTCACATGTCGAAGTCATTTTCTGGATCAGAATCTCAATCTGTTGATAATGCTGGTAGAGTGCTGAGGCCCAGGGCCAGGAAGCAGCACAAAGAAAAGAAAGGCACAAGGAAAAGGCGCCACTTCTATGAAATCTTCTTCGGGGACTTGGATGCTTGTTGGGTATTGAACCGTAGGATCAAGGTCTTCTGGCCTTTGGACCAAAGTTGGTATTATGGCCTTGTGAATGATTATGACAAGGAGAAAAAACTTCACCATATTAGATATGATGACCGTGAAGAAGAATGGGTTGATCTTCAGCATGAGCGGTTCAAGCTCTTGCTACTTCCTACTGAAGTTCCTGGTAAGGCAAAGCAGGAAAAATCAATCATGCAAATTACAAGTTCTGAAGAGAGAGAAGGAAACTTGAAACCCAGAAAAGAGAAGAAAAAGAGAGACTCGATGTCGGAGGACGATAGCTGCATAGGCAGCTGTATGGATTCTGAACCTATTATTTCATGGTTGTCGCGTCGGATCAAGTCTCCTTCTCATGCTGTGAAGAAGCAAAAAACAACTGGTCTATCTCCAAAGTCCGTGCCAACTTTGTCTGACAGTGCTGGCACTTTACATGGGTGTTTGAGTGATGTTTCCTCTAGTAGGGATACAAATAAATTATTTTGTAATTCTGGCAGATCATCTGATCCTGTGAGGGAAGAGAAGCCTGCTCCAGAGGGCAACTCCGAAGATAGCAAAATGCCTATTGTTTATTATCGGAAGCGATTGCGCAAGACTGGCTCTGTACTGTCTCAAATATACAAGGATGAGCATGCTTCCATGCATGGACATCATTGTTGTACTTCTGTTACTCCTGTTGAGGAGGTTTGGGACTTGGAAGAGCCAGATGATTTTGTTGTTATATTGGATCGCAGTTGGCCATTGTGGTATAGTGATGATGCAGGGTTGCTAAAGTTAACTCTTCCTTGGATAGAATCAGGAAAAGTCAAATTTAAATGCCTTCCACTGCATTCACTAATGAATGATTCTCTTGGAGTAGAGTTGTTACGGTTCTTTCATGCTGCAATGCTACTTCGGCATGGTGTAGTGGTGACCACATGGCCAAAGGTTCATTTGGAGATGCTCTTTGTTGATAATGTAGTTGGACTGAGGTTTCTTTTGTTTGAAGGTTGCTTGAAGCAGGCGGTGGCCTTTGTTTCTCTTATCCTGATATTATTTCATCACCCTAATGAACAGGGGAAGCTAACTGATTTCCAGTTACCAGCAACTTCAATCAGGTTTAAATTCTCCTGTGTTCAGCATCTTGGAAAGCAACTTGTGTCTGCTTTCTACAACTTCTGTCAAGTTAAAAATTCAACGTGGATGTACCTAGACAATAAACTCAGGAGGCATTGTTTACTTGCCAAAAAACTGCCTCTGTCTGAGTGCACCTATGATAATATCCAGGCACTTCAAAATGGAATAAATCAGTCACCTTCTATGTCTCGTTGCGGGCAACCCTCTGTCAAGGGTACACGGAAGAGGTCCAGGCAGGGCATTAACTTCATGGGCAGTTCTCGGGAAGTTAGTTTTGTAAATATCAGCCATTCTGCTATTCATTCTGATGAGCTTCACAGAAAGCTTCCCCCACTTGCTCTATCTTTTGCAGCTGCACCTACTTTCTTCATTAATTTGCATCTTAAGTTGCTTATGGAACACAGCGTTGCTAATATCTGCTTTCAAGACCATGATTCAGAAATTACTCCCGAGAACAATTTGAAGGCTCCAGCAACTGTGGCTACCTCTGGTGGGTCCTGTACTAAAGTGGTAACAGAAACATCTCTTTCTATTTGCAGTCAGCGCGGTCGGTTAAAGTCATCTCAGCTTTACCAAAATTGTGTTGTTAATGTTGCTGGAGCCTCTTCTAGAACTTTCACTGGCAGAGATAAATCCGAAACTAGTTCCCGTTCTATTGTGAATGGGCTTACTGTTGAAATTCCATCTTTTGACCATTTTGAGAAGCCTGTGGAGAGGGAGTTACAAAGTGCTCAACAGCCTACAGTTTTTTCATTGAATATGAATGGTAGCATTATCCCCAGTCCCAGCCCCACTGCTCCCAGAAGTACAGGGCTGCGAAATAGAAATAGTATGTCATCATTTGGAAACCTCGCACATCGTTGGTCAGATGGAAAGGCTGACATTTTCCATAATGGTTTTGGTAATGGACCGAAGAAGCCCCGAACTCAAGTCTCATACACATTGCCTTGTGGAGGTTTTGATGTCAGCTCTAAGCAAAGAAATGTTCACAAAGGGCTTCCTAATAAACGAATTAGAAGGGCCAGCGAGAAGAGGTCATCAGACATTTCCAGAGGTTCCGAAAGGAACTTGGAGCTCTTATCCTGTGAGGCAAATGTGTTAATTTCTGCAAGTGACAGAGGGTGGAGAGAATGTGGGGCACGTGTAGTATTAGAACTCTTTGATAATAGTGAGTGGAAGCTTGCTGTAAAACTTTCAGGGACTACGAAGTATTCATACAAAGCACATCAATTTTTGCAGCCTGGGTCAACAAACCGCTATACTCATGTTATGATGTGGAAAGGAGGAAAGGATTGGATCTTGGAGTTTCCAGATCGGAGCCAGTGGGCCCTTTTCAAGGAGATGCATGAAGAGTGTTACAATCGGAACCTTCGGTCTGCGTCAGTTAAAAACATTCCTATTCCTGGTGTTCGCTTAGTAGAAGATATTGATGATAATGGAACAGAAATAGCATTTCTTCGCAGCTCTACCAAGTACTTTCAGCAGATGAAAACAGATGTTGAGATGGCTTTAGATCCATCTTGTCTCTTGTATGACATGGATAGTGATGATGAGCGATGGATTTTGAAGTTTCAAAATTCTTCAGAAGTTGAAAAGAGTAGCTCAATGGAGATTGGCGAGGAGATGTTTGAGAAGACAGTGGACATGTTTGAGAAGGCTGCATATGTTCGGCAATGTGATCAGTTCACATCTGAAGAAATAGAAGAGTTCATGACTGGTATTGGGCCCATGGATTTGATTAAAACCATTTATGAGCATTGGCGTCAGAAACGGCTGAGAAAGGGAATGCCTTTAATCCGACATCTCCAGCCACCGTCATGGGAGACGTATCAAAAACAAGTGAGGGAGTGGGAGCAAGCTATGACCAAGATGAATAACACACTCGCAAATGGTGGCCGTGAGAAAGCTGCAGCAGTTGAGAAGCCACCGATGTATGCTTTTTGTCTGAAACCACGGGGCCTCGAAGTTCCCAATAAGGGATCAAAGCAACGGTCACAGAAGAAATTTTCTATTTCTGCACATACCAATGCGGTGTTAGGAGATCAGGATGGTTTCCATCCTATTGGAAGGAGATCAAATGGGTTTGCCTATGGTGATGAAAAAATTGCATATTCAGGCCATAACTATGAATCTTTAGATGACTCTCCATTGTCTCAGTCGTCGCCCAGGGTTTTTTCACCAAGGGATGCAGCAAACATCTGTATGAGCAATGATGGTTTTGAGAGGAATCATCTTCACAGAATTGACAGAAGCAAGTCAAAGAAATATCGGACAATAGCATCCTCTGTTGACCCCCAGATGGTGTCTCCATATAATCATAGAGTGGTACGAAACAGAAATGGAGTTCATAGAGGGAATATTGGTTTCCCGGAGTGGTCAAGCCAGTCATATTATCAACCAGATGTTGCTCAGAGGCTTGTAAATGCGCATGGCTTTGATCCTGATGAGTTTAGGCTTCGTGAGGCATCCGGTGCTGCTCAGCATGCACATAACTTAGCCAAGCGTAAGAGAGAAAATGCTCGCAGATTGTTTTACAGAGCAGATCTAGCAATGCACAAGGCTGTGGTTGCTCTCATGACTGCAGAAGCAATCAAAGCTTCTTCAGAGGACTACGACTATGACTACCACTCTGAGTCCGACTCCTTCTCCATCTCCAACTCTGACACCGATTCAGACAGTGAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPL1

Protein Analysis

1755

Amino Acids

198.06

Weight (kDa)

9.1

Isoelectric Point (pI)

58.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPL1 PF10513 1316 - 1411 1.3e-06 Enhancer of polycomb-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 2051, 5004
AccB1I GGYRCC 1 cut(s) 1404
AccBSI CCGCTC 1 cut(s) 1594
AccII CGCG 3 cut(s) 113, 1795, 3123
AccIII TCCGGA 1 cut(s) 625
AciI CCGC 6 cut(s) 1594, 2465, 2772, 3123, 3832, 4607
AcoI YGGCCR 3 cut(s) 2198, 2381, 4471
AcsI RAATTY 7 cut(s) 387, 2270, 2564, 2632, 3255, 4153, 4580
AcuI CTGAAG 7 cut(s) 488, 1568, 1641, 1695, 4143, 4290, 5163
AcvI CACGTG 2 cut(s) 91, 3719
AcyI GRCGYC 3 cut(s) 329, 1405, 4339
AfiI CCNNNNNNNGG 8 cut(s) 1365, 1923, 3069, 3530, 3913, 3980, 4553, 4876
AflII CTTAAG 2 cut(s) 416, 2960
AflIII ACRYGT 3 cut(s) 1293, 3718, 4221
AhdI GACNNNNNGTC 2 cut(s) 314, 1729
AhlI ACTAGT 3 cut(s) 560, 812, 3218
Alw21I GWGCWC 4 cut(s) 772, 2711, 3310, 3654
Alw44I GTGCAC 1 cut(s) 2707
AlwNI CAGNNNCTG 2 cut(s) 466, 3053
Ama87I CYCGRG 3 cut(s) 724, 2838, 3026
Aor13HI TCCGGA 1 cut(s) 625
ApaI GGGCCC 2 cut(s) 3907, 4305
ApaLI GTGCAC 1 cut(s) 2707
ApoI RAATTY 7 cut(s) 387, 2270, 2564, 2632, 3255, 4153, 4580
ArsI GACNNNNNNTTYG 4 cut(s) 1238, 1270, 4553, 4585
AseI ATTAAT 1 cut(s) 2949
Asp700I GAANNNNTTC 1 cut(s) 969
AspLEI GCGC 5 cut(s) 87, 1407, 2052, 3123, 5005
AsuC2I CCSGG 2 cut(s) 1024, 4946
AsuII TTCGAA 1 cut(s) 209
AvaI CYCGRG 3 cut(s) 724, 2838, 3026
AvaII GGWCC 4 cut(s) 1486, 2805, 3072, 3485
AxyI CCTNAGG 1 cut(s) 194
BaeGI GKGCMC 4 cut(s) 2711, 3718, 3907, 4305
BalI TGGCCA 2 cut(s) 2200, 2383
BanI GGYRCC 1 cut(s) 1404
BanII GRGCYC 3 cut(s) 3654, 3907, 4305
BarI GAAGNNNNNNTAC 2 cut(s) 3776, 3808
BbrPI CACGTG 2 cut(s) 91, 3719
BbsI GAAGAC 3 cut(s) 1140, 1465, 4217
Bbv12I GWGCWC 4 cut(s) 772, 2711, 3310, 3654
BbvCI CCTCAGC 2 cut(s) 873, 1352
BceAI ACGGC 2 cut(s) 4364, 4458
BcgI CGANNNNNNTGC 2 cut(s) 621, 655
BciVI GTATCC 1 cut(s) 1921
BclI TGATCA 2 cut(s) 820, 4255
BcnI CCSGG 2 cut(s) 1024, 4946
BcuI ACTAGT 3 cut(s) 560, 812, 3218
BfaI CTAG 8 cut(s) 173, 561, 813, 1920, 2651, 3186, 3219, 5123
BfmI CTRYAG 3 cut(s) 3318, 4485, 5157
BfoI RGCGCY 1 cut(s) 1408
BfrI CTTAAG 2 cut(s) 416, 2960
BfuI GTATCC 1 cut(s) 1921
BglI GCCNNNNNGGC 1 cut(s) 884
BglII AGATCT 2 cut(s) 404, 5119
BlpI GCTNAGC 3 cut(s) 771, 1086, 5055
BmcAI AGTACT 1 cut(s) 4052
Bme18I GGWCC 4 cut(s) 1486, 2805, 3072, 3485
BmeRI GACNNNNNGTC 2 cut(s) 314, 1729
BmeT110I CYCGRG 3 cut(s) 724, 2838, 3026
BmrI ACTGGG 2 cut(s) 1212, 3351
BmuI ACTGGG 2 cut(s) 1212, 3351
BpiI GAAGAC 3 cut(s) 1140, 1465, 4217
BplI GAGNNNNNCTC 4 cut(s) 1988, 2020, 5201, 5233
BpmI CTGGAG 4 cut(s) 1977, 3030, 3195, 4367
Bpu10I CCTNAGC 2 cut(s) 873, 1352
Bpu1102I GCTNAGC 3 cut(s) 771, 1086, 5055
Bpu14I TTCGAA 1 cut(s) 209
BpuEI CTTGAG 4 cut(s) 530, 852, 995, 3489
BpuMI CCSGG 2 cut(s) 1024, 4946
BsaAI YACGTR 2 cut(s) 91, 3719
BsaBI GATNNNNATC 3 cut(s) 210, 825, 1318
BsaHI GRCGYC 3 cut(s) 329, 1405, 4339
BsaWI WCCGGW 2 cut(s) 625, 5045
Bsc4I CCNNNNNNNGG 8 cut(s) 1365, 1923, 3069, 3530, 3913, 3980, 4553, 4876
Bse118I RCCGGY 1 cut(s) 800
Bse1I ACTGG 9 cut(s) 1207, 1846, 2062, 2536, 3202, 3357, 3898, 4297, 4960
Bse21I CCTNAGG 1 cut(s) 194
Bse3DI GCAATG 6 cut(s) 1003, 2352, 3518, 4256, 4792, 5133
Bse8I GATNNNNATC 3 cut(s) 210, 825, 1318
BseAI TCCGGA 1 cut(s) 625
BseJI GATNNNNATC 3 cut(s) 210, 825, 1318
BseLI CCNNNNNNNGG 8 cut(s) 1365, 1923, 3069, 3530, 3913, 3980, 4553, 4876
BseMI GCAATG 6 cut(s) 1003, 2352, 3518, 4256, 4792, 5133
BseNI ACTGG 9 cut(s) 1207, 1846, 2062, 2536, 3202, 3357, 3898, 4297, 4960
BseRI GAGGAG 5 cut(s) 529, 1154, 1157, 2156, 4211
BseSI GKGCMC 4 cut(s) 2711, 3718, 3907, 4305
BseYI CCCAGC 1 cut(s) 3362
BsgI GTGCAG 2 cut(s) 2916, 4578
Bsh1236I CGCG 3 cut(s) 113, 1795, 3123
Bsh1285I CGRYCG 2 cut(s) 125, 3127
BshNI GGYRCC 1 cut(s) 1404
BsiEI CGRYCG 2 cut(s) 125, 3127
BsiHKAI GWGCWC 4 cut(s) 772, 2711, 3310, 3654
BsiHKCI CYCGRG 3 cut(s) 724, 2838, 3026
BsiSI CCGG 6 cut(s) 203, 626, 801, 1024, 4946, 5046
BslFI GGGAC 6 cut(s) 515, 1006, 1445, 2165, 3345, 3790
BslI CCNNNNNNNGG 8 cut(s) 1365, 1923, 3069, 3530, 3913, 3980, 4553, 4876
BsmBI CGTCTC 2 cut(s) 336, 4394
BsmFI GGGAC 6 cut(s) 515, 1006, 1445, 2165, 3345, 3790
BsmI GAATGC 7 cut(s) 632, 714, 917, 946, 1165, 2290, 4368
BsoBI CYCGRG 3 cut(s) 724, 2838, 3026
Bsp119I TTCGAA 1 cut(s) 209
Bsp120I GGGCCC 2 cut(s) 3903, 4301
Bsp1286I GDGCHC 7 cut(s) 772, 2711, 3310, 3654, 3718, 3907, 4305
Bsp13I TCCGGA 1 cut(s) 625
Bsp1407I TGTACA 1 cut(s) 426
Bsp1720I GCTNAGC 3 cut(s) 771, 1086, 5055
Bsp19I CCATGG 1 cut(s) 4305
BspACI CCGC 6 cut(s) 1594, 2465, 2772, 3123, 3832, 4607
BspEI TCCGGA 1 cut(s) 625
BspFNI CGCG 3 cut(s) 113, 1795, 3123
BspHI TCATGA 3 cut(s) 243, 4287, 5151
BspMAI CTGCAG 2 cut(s) 4489, 5161
BspQI GCTCTTC 1 cut(s) 2154
BspT104I TTCGAA 1 cut(s) 209
BspT107I GGYRCC 1 cut(s) 1404
BspTI CTTAAG 2 cut(s) 416, 2960
BsrBI CCGCTC 1 cut(s) 1594
BsrDI GCAATG 6 cut(s) 1003, 2352, 3518, 4256, 4792, 5133
BsrFI RCCGGY 1 cut(s) 800
BsrGI TGTACA 1 cut(s) 426
BsrI ACTGG 9 cut(s) 1207, 1846, 2062, 2536, 3202, 3357, 3898, 4297, 4960
BssAI RCCGGY 1 cut(s) 800
BssNI GRCGYC 3 cut(s) 329, 1405, 4339
BssT1I CCWWGG 4 cut(s) 1221, 2246, 4305, 4757
BstACI GRCGYC 3 cut(s) 329, 1405, 4339
BstAFI CTTAAG 2 cut(s) 416, 2960
BstAPI GCANNNNNTGC 1 cut(s) 5048
BstAUI TGTACA 1 cut(s) 426
BstBAI YACGTR 2 cut(s) 91, 3719
BstBI TTCGAA 1 cut(s) 209
BstDSI CCRYGG 2 cut(s) 4305, 4529
BstEII GGTNACC 1 cut(s) 2371
BstENI CCTNNNNNAGG 3 cut(s) 1921, 3528, 3911
BstFNI CGCG 3 cut(s) 113, 1795, 3123
BstH2I RGCGCY 1 cut(s) 1408
BstHHI GCGC 5 cut(s) 87, 1407, 2052, 3123, 5005
BstMCI CGRYCG 2 cut(s) 125, 3127
BstNSI RCATGY 7 cut(s) 305, 956, 1119, 1297, 2096, 4225, 5063
BstPI GGTNACC 1 cut(s) 2371
BstSFI CTRYAG 3 cut(s) 3318, 4485, 5157
BstSLI GKGCMC 4 cut(s) 2711, 3718, 3907, 4305
BstUI CGCG 3 cut(s) 113, 1795, 3123
BstV2I GAAGAC 3 cut(s) 1140, 1465, 4217
BstX2I RGATCY 5 cut(s) 404, 412, 3873, 4090, 5119
BstXI CCANNNNNNTGG 2 cut(s) 4395, 4640
BstYI RGATCY 5 cut(s) 404, 412, 3873, 4090, 5119
Bsu36I CCTNAGG 1 cut(s) 194
BsuI GTATCC 1 cut(s) 1921
BtgI CCRYGG 2 cut(s) 4305, 4529
BtgZI GCGATG 3 cut(s) 276, 1247, 4147
BtsI GCAGTG 2 cut(s) 2285, 3369
BtsIMutI CAGTG 9 cut(s) 35, 381, 1885, 2285, 3195, 3369, 3905, 4221, 5260
CaiI CAGNNNCTG 2 cut(s) 466, 3053
CciI TCATGA 3 cut(s) 243, 4287, 5151
CfoI GCGC 5 cut(s) 87, 1407, 2052, 3123, 5005
Cfr10I RCCGGY 1 cut(s) 800
CseI GACGC 6 cut(s) 318, 638, 1091, 1784, 3945, 4328
DinI GGCGCC 1 cut(s) 1406
DraI TTTAAA 2 cut(s) 2275, 2563
DriI GACNNNNNGTC 2 cut(s) 314, 1729
EaeI YGGCCR 3 cut(s) 2198, 2381, 4471
Eam1105I GACNNNNNGTC 2 cut(s) 314, 1729
Ecl136II GAGCTC 1 cut(s) 3652
Eco130I CCWWGG 4 cut(s) 1221, 2246, 4305, 4757
Eco147I AGGCCT 1 cut(s) 878
Eco24I GRGCYC 3 cut(s) 3654, 3907, 4305
Eco47I GGWCC 4 cut(s) 1486, 2805, 3072, 3485
Eco53kI GAGCTC 1 cut(s) 3652
Eco57I CTGAAG 7 cut(s) 488, 1568, 1641, 1695, 4143, 4290, 5163
Eco72I CACGTG 2 cut(s) 91, 3719
Eco81I CCTNAGG 1 cut(s) 194
Eco88I CYCGRG 3 cut(s) 724, 2838, 3026
Eco91I GGTNACC 1 cut(s) 2371
EcoICRI GAGCTC 1 cut(s) 3652
EcoNI CCTNNNNNAGG 3 cut(s) 1921, 3528, 3911
EcoO109I RGGNCCY 3 cut(s) 3072, 3904, 4534
EcoO65I GGTNACC 1 cut(s) 2371
EcoT14I CCWWGG 4 cut(s) 1221, 2246, 4305, 4757
EcoT22I ATGCAT 2 cut(s) 2106, 3924
EcoT38I GRGCYC 3 cut(s) 3654, 3907, 4305
EgeI GGCGCC 1 cut(s) 1406
EheI GGCGCC 1 cut(s) 1406
ErhI CCWWGG 4 cut(s) 1221, 2246, 4305, 4757
Esp3I CGTCTC 2 cut(s) 336, 4394
FaqI GGGAC 6 cut(s) 515, 1006, 1445, 2165, 3345, 3790
FauI CCCGC 1 cut(s) 2765
FauNDI CATATG 1 cut(s) 4240
FbaI TGATCA 2 cut(s) 820, 4255
FriOI GRGCYC 3 cut(s) 3654, 3907, 4305
FspAI RTGCGCAY 1 cut(s) 5004
FspBI CTAG 8 cut(s) 173, 561, 813, 1920, 2651, 3186, 3219, 5123
FspI TGCGCA 2 cut(s) 2051, 5004
GlaI GCGC 5 cut(s) 86, 1406, 2051, 3122, 5004
GsaI CCCAGC 1 cut(s) 3366
GsuI CTGGAG 4 cut(s) 1977, 3030, 3195, 4367
HaeII RGCGCY 1 cut(s) 1408
HapII CCGG 6 cut(s) 203, 626, 801, 1024, 4946, 5046
HgaI GACGC 6 cut(s) 318, 638, 1091, 1784, 3945, 4328
HhaI GCGC 5 cut(s) 87, 1407, 2052, 3123, 5005
Hin1I GRCGYC 3 cut(s) 329, 1405, 4339
Hin6I GCGC 5 cut(s) 85, 1405, 2050, 3121, 5003
HinP1I GCGC 5 cut(s) 85, 1405, 2050, 3121, 5003
HincII GTYRAC 3 cut(s) 2238, 3825, 4867
HindII GTYRAC 3 cut(s) 2238, 3825, 4867
HindIII AAGCTT 4 cut(s) 692, 2900, 3755, 5172
HpaI GTTAAC 1 cut(s) 2238
HpaII CCGG 6 cut(s) 203, 626, 801, 1024, 4946, 5046
Hpy99I CGWCG 4 cut(s) 494, 497, 1800, 4741
HpyCH4IV ACGT 5 cut(s) 48, 90, 2639, 3718, 4403
HpySE526I ACGT 5 cut(s) 48, 90, 2639, 3718, 4403
Hsp92I GRCGYC 3 cut(s) 329, 1405, 4339
HspAI GCGC 5 cut(s) 85, 1405, 2050, 3121, 5003
KasI GGCGCC 1 cut(s) 1404
Kpn2I TCCGGA 1 cut(s) 625
Ksp22I TGATCA 2 cut(s) 820, 4255
KspAI GTTAAC 1 cut(s) 2238
LguI GCTCTTC 1 cut(s) 2154
LmnI GCTCC 7 cut(s) 1996, 3049, 3176, 3379, 3649, 3894, 4429
MaeI CTAG 8 cut(s) 173, 561, 813, 1920, 2651, 3186, 3219, 5123
MaeII ACGT 5 cut(s) 48, 90, 2639, 3718, 4403
MbiI CCGCTC 1 cut(s) 1594
MflI RGATCY 5 cut(s) 404, 412, 3873, 4090, 5119
MhlI GDGCHC 7 cut(s) 772, 2711, 3310, 3654, 3718, 3907, 4305
MlsI TGGCCA 2 cut(s) 2200, 2383
MluNI TGGCCA 2 cut(s) 2200, 2383
Mly113I GGCGCC 1 cut(s) 1405
MlyI GAGTC 5 cut(s) 803, 1718, 4712, 5210, 5219
Mox20I TGGCCA 2 cut(s) 2200, 2383
Mph1103I ATGCAT 2 cut(s) 2106, 3924
MroI TCCGGA 1 cut(s) 625
MroXI GAANNNNTTC 1 cut(s) 969
MscI TGGCCA 2 cut(s) 2200, 2383
MslI CAYNNNNRTG 7 cut(s) 1413, 2508, 2883, 4393, 4602, 4701, 4778
Msp20I TGGCCA 2 cut(s) 2200, 2383
MspA1I CMGCKG 3 cut(s) 775, 1758, 2930
MspCI CTTAAG 2 cut(s) 416, 2960
MspI CCGG 6 cut(s) 203, 626, 801, 1024, 4946, 5046
Mva1269I GAATGC 7 cut(s) 632, 714, 917, 946, 1165, 2290, 4368
MvnI CGCG 3 cut(s) 113, 1795, 3123
NarI GGCGCC 1 cut(s) 1405
NciI CCSGG 2 cut(s) 1024, 4946
NcoI CCATGG 1 cut(s) 4305
NdeI CATATG 1 cut(s) 4240
NmeAIII GCCGAG 1 cut(s) 83
NmuCI GTSAC 7 cut(s) 32, 1053, 1289, 2371, 2751, 3689, 4569
NsbI TGCGCA 2 cut(s) 2051, 5004
NsiI ATGCAT 2 cut(s) 2106, 3924
NspI RCATGY 7 cut(s) 305, 956, 1119, 1297, 2096, 4225, 5063
NspV TTCGAA 1 cut(s) 209
PaeI GCATGC 3 cut(s) 1119, 2096, 5063
PagI TCATGA 3 cut(s) 243, 4287, 5151
PasI CCCWGGG 2 cut(s) 1359, 4743
PceI AGGCCT 1 cut(s) 878
PciI ACATGT 2 cut(s) 1293, 4221
PciSI GCTCTTC 1 cut(s) 2154
PctI GAATGC 7 cut(s) 632, 714, 917, 946, 1165, 2290, 4368
PdmI GAANNNNTTC 1 cut(s) 969
PfoI TCCNGGA 3 cut(s) 434, 1022, 4944
Ple19I CGATCG 1 cut(s) 125
PleI GAGTC 5 cut(s) 802, 1718, 4712, 5210, 5218
PluTI GGCGCC 1 cut(s) 1408
PmaCI CACGTG 2 cut(s) 91, 3719
PmlI CACGTG 2 cut(s) 91, 3719
PpsI GAGTC 5 cut(s) 802, 1718, 4712, 5210, 5218
Ppu21I YACGTR 2 cut(s) 91, 3719
PpuMI RGGWCCY 1 cut(s) 3072
PscI ACATGT 2 cut(s) 1293, 4221
PshBI ATTAAT 1 cut(s) 2949
Psp124BI GAGCTC 1 cut(s) 3654
Psp5II RGGWCCY 1 cut(s) 3072
PspCI CACGTG 2 cut(s) 91, 3719
PspEI GGTNACC 1 cut(s) 2371
PspFI CCCAGC 1 cut(s) 3362
PspOMI GGGCCC 2 cut(s) 3903, 4301
PspPPI RGGWCCY 1 cut(s) 3072
PstI CTGCAG 2 cut(s) 4489, 5161
PstNI CAGNNNCTG 2 cut(s) 466, 3053
PsuI RGATCY 5 cut(s) 404, 412, 3873, 4090, 5119
PvuI CGATCG 1 cut(s) 125
PvuII CAGCTG 3 cut(s) 775, 1758, 2930
RseI CAYNNNNRTG 7 cut(s) 1413, 2508, 2883, 4393, 4602, 4701, 4778
SacI GAGCTC 1 cut(s) 3654
SapI GCTCTTC 1 cut(s) 2154
ScaI AGTACT 1 cut(s) 4052
SchI GAGTC 5 cut(s) 803, 1718, 4712, 5210, 5219
SduI GDGCHC 7 cut(s) 772, 2711, 3310, 3654, 3718, 3907, 4305
SfcI CTRYAG 3 cut(s) 3318, 4485, 5157
SfiI GGCCNNNNNGGCC 1 cut(s) 884
SfoI GGCGCC 1 cut(s) 1406
SfuI TTCGAA 1 cut(s) 209
SinI GGWCC 4 cut(s) 1486, 2805, 3072, 3485
SmiI ATTTAAAT 1 cut(s) 2275
SmiMI CAYNNNNRTG 7 cut(s) 1413, 2508, 2883, 4393, 4602, 4701, 4778
SmlI CTYRAG 6 cut(s) 416, 545, 867, 974, 2960, 3504
SmoI CTYRAG 6 cut(s) 416, 545, 867, 974, 2960, 3504
SpeI ACTAGT 3 cut(s) 560, 812, 3218
SphI GCATGC 3 cut(s) 1119, 2096, 5063
SseBI AGGCCT 1 cut(s) 878
SsiI CCGC 6 cut(s) 1594, 2465, 2772, 3123, 3832, 4607
SspDI GGCGCC 1 cut(s) 1404
SspI AATATT 1 cut(s) 4936
SspMI CTAG 8 cut(s) 173, 561, 813, 1920, 2651, 3186, 3219, 5123
SstI GAGCTC 1 cut(s) 3654
StuI AGGCCT 1 cut(s) 878
StyI CCWWGG 4 cut(s) 1221, 2246, 4305, 4757
SwaI ATTTAAAT 1 cut(s) 2275
TaiI ACGT 5 cut(s) 51, 93, 2642, 3721, 4406
TaqI TCGA 8 cut(s) 121, 125, 209, 401, 1181, 1298, 1727, 4539
TaqII GACCGA 2 cut(s) 3502, 3939
TatI WGTACW 7 cut(s) 426, 1199, 2065, 2120, 3077, 3383, 4050
TscAI CASTG 9 cut(s) 35, 388, 1885, 2292, 3202, 3376, 3905, 4221, 5260
TseFI GTSAC 7 cut(s) 32, 1053, 1289, 2371, 2751, 3689, 4569
Tsp45I GTSAC 7 cut(s) 32, 1053, 1289, 2371, 2751, 3689, 4569
TspGWI ACGGA 4 cut(s) 29, 325, 1849, 2812
TspRI CASTG 9 cut(s) 35, 388, 1885, 2292, 3202, 3376, 3905, 4221, 5260
Vha464I CTTAAG 2 cut(s) 416, 2960
VneI GTGCAC 1 cut(s) 2707
VpaK11BI GGWCC 4 cut(s) 1486, 2805, 3072, 3485
VspI ATTAAT 1 cut(s) 2949
XagI CCTNNNNNAGG 3 cut(s) 1921, 3528, 3911
XapI RAATTY 7 cut(s) 387, 2270, 2564, 2632, 3255, 4153, 4580
XbaI TCTAGA 1 cut(s) 3185
XceI RCATGY 7 cut(s) 305, 956, 1119, 1297, 2096, 4225, 5063
XcmI CCANNNNNNNNNTGG 1 cut(s) 3473
XmnI GAANNNNTTC 1 cut(s) 969
XspI CTAG 8 cut(s) 173, 561, 813, 1920, 2651, 3186, 3219, 5123
ZrmI AGTACT 1 cut(s) 4052
Zsp2I ATGCAT 2 cut(s) 2106, 3924
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.