FvH4_2g31250
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0014670
Physical Location & Seq
Reverse (-)
24004403 .. 24006750
2348 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g31250.t1

Sequence Viewer

Length: 1302 bp
ATGAACAAACTTCAAGGGATATTTCAGAAGAAAAAGGAGTCTTTAAAGGCGTCCCCGAGGATTGAGCGTACCTGTTACACGGGGGCTGTGGAGCCTCACGGTTGTTGTCGAGAGTCGGTTGACGTTGTTTCGATGAGCACTACCAACAAGTTGGAACAGCAGGTGGCAGAACTGGAAAAGAAACTTGAAAAGGAGAGAGAACTGCGGATGATGTACAGAAAGAAATTGGAAAGAACACAAGATTATTTGAGGTATTGTCTACAAGTAGCCCAAGAAAATGGGTTTTTGGACCTTATACTCCACAACACAGATCATGCCGGCCTTGTTAACGATGCCAATGGCCATCCCAGTCCACAGTTGCCAACTACTCCTCTGCATCAGCACCAACACAACGAACCTTCGGATCTAGATGCTCTGACTGATCAAGCCAAGCTAAATGGATGGTACATAGATCCAGATGAGATTGAATTCGAAGAGAAGATAGGGCAGGGGAGCACAGCGCAGATATACAAAGCAACATGGCATGGACTTGATGTTGCGGTGAAATGCATAGAACCTGACTTCTTTGAATCCAACGAGAATGGTGTCTCATTTTTCGCTCAAGAGCTCGAAACGTTATGCAGGCAGCGCCATCGGTATGTGCTTCATCTAATGGGGGCGTGCCTTGATCCTCCTAACTATGCTTGGGTGGTGACCGAGTTCTTGAGCTCCACATTGAAAGAGTGGCTGCATGGCCCGGGAAATAGGCAAAAAGAGAGGATGATTGAGCTTCCTCCATTAGCAGAGAGGGTGGCCAAGGCACTGGAGATTGCTCAGGCCATGCAGTATCTCCATGAACAAAAGCCTAAGGTTCTTCATCGTGACCTAAAGCCTAGCAACATCTTCTTGGACGATGCTGGTCACATTAGAGTAGCGGATTTCGGTCACGCTCGGTTCTTGAGTGATGAGGAGATGGCACTAACAGGGGAAACAGGAACATATGTGTATATGGCACCAGAGGTGATCCAGTGCGAGCCTTATAATGAGAAATGTGATGTATACAGCTTCGGGATTATACTCAATGAGATAATAACTGGAAATTATCCATACATTGAGACAGATTATGGTCCTACTATGATTGCAATGGAAGTTGTTGAGGGTAAACTGAGGCCAAGACTTCCCGATGACGACGATGGCCAACTTGGGGAGCTAATTGAGCTGATTTGCCTTTCATGGGATGGAGATGCTTCCATCAGACCATCCTTTGACACCATCACTTGCAGTCTGAAGAAGCTTCAATACATAATAGTCTACAATCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

434

Amino Acids

49.51

Weight (kDa)

5.1

Isoelectric Point (pI)

37.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 155 - 421 8.1e-42 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 156 - 367 1.8e-43 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014670)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g31250
malus_domestica MD08G1069800.v1.1 MD15G1052200.v1.1
prunus_persica Prupe.1G406200_v2.0.a1 Prupe.1G406200_v2.0.a1
pyrus_communis pycom15g05000
rosa_chinensis RchiOBHm_Chr6g0311551
rosa_laevigata RLG00000010394
rosa_multiflora Rmu_co8269907.1_g000001 Rmu_sc0007556.1_g000001
rosa_roxburghii Rroxscaffold_7G00157500
rosa_rugosa Rorug06G0389700
rosa_samantha Rh6AG504600 Rh6BG515300 Rh6CG521200 Rh6DG507100
rosa_wichuraiana Rw6G043940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1020
AarI CACCTGC 1 cut(s) 151
Acc36I ACCTGC 1 cut(s) 151
AccB1I GGYRCC 1 cut(s) 991
AccI GTMKAC 3 cut(s) 259, 1038, 1290
AciI CCGC 3 cut(s) 205, 539, 914
AclI AACGTT 1 cut(s) 614
AclWI GGATC 4 cut(s) 411, 446, 662, 997
AcoI YGGCCR 3 cut(s) 340, 792, 1174
AcsI RAATTY 1 cut(s) 467
AcuI CTGAAG 1 cut(s) 1286
AcyI GRCGYC 1 cut(s) 50
AfaI GTAC 3 cut(s) 70, 215, 446
AfiI CCNNNNNNNGG 2 cut(s) 1183, 1213
AgsI TTSAA 6 cut(s) 14, 188, 467, 569, 718, 1277
AluBI AGCT 8 cut(s) 433, 607, 708, 769, 1044, 1189, 1198, 1273
AluI AGCT 8 cut(s) 433, 607, 708, 769, 1044, 1189, 1198, 1273
Alw21I GWGCWC 4 cut(s) 140, 497, 609, 710
Alw26I GTCTC 2 cut(s) 592, 1088
AlwI GGATC 4 cut(s) 411, 446, 662, 997
Ama87I CYCGRG 2 cut(s) 55, 736
AoxI GGCC 7 cut(s) 319, 340, 733, 792, 816, 1148, 1174
ApeKI GCWGC 2 cut(s) 625, 727
ApoI RAATTY 1 cut(s) 467
AspLEI GCGC 2 cut(s) 502, 630
AspS9I GGNCC 3 cut(s) 289, 734, 1106
AsuC2I CCSGG 2 cut(s) 737, 738
AsuHPI GGTGA 3 cut(s) 553, 703, 1012
AsuII TTCGAA 1 cut(s) 471
AvaI CYCGRG 2 cut(s) 55, 736
AvaII GGWCC 2 cut(s) 289, 1106
AxyI CCTNAGG 1 cut(s) 846
BalI TGGCCA 3 cut(s) 342, 794, 1176
BanI GGYRCC 1 cut(s) 991
BanII GRGCYC 2 cut(s) 609, 710
BarI GAAGNNNNNNTAC 2 cut(s) 1262, 1294
Bbv12I GWGCWC 4 cut(s) 140, 497, 609, 710
BbvI GCAGC 2 cut(s) 637, 714
BccI CCATC 9 cut(s) 351, 435, 639, 946, 1166, 1211, 1238, 1246, 1259
BcgI CGANNNNNNTGC 2 cut(s) 614, 648
BclI TGATCA 1 cut(s) 421
BcnI CCSGG 2 cut(s) 737, 738
BcoDI GTCTC 2 cut(s) 592, 1088
BfaI CTAG 2 cut(s) 407, 873
BfoI RGCGCY 1 cut(s) 631
BfuAI ACCTGC 1 cut(s) 151
BisI GCNGC 2 cut(s) 626, 728
BlsI GCNGC 2 cut(s) 627, 729
Bme1390I CCNGG 2 cut(s) 737, 738
Bme18I GGWCC 2 cut(s) 289, 1106
BmeT110I CYCGRG 2 cut(s) 55, 736
BmgT120I GGNCC 3 cut(s) 289, 734, 1106
BmiI GGNNCC 2 cut(s) 93, 993
BmrFI CCNGG 2 cut(s) 737, 738
BmrI ACTGGG 1 cut(s) 342
BmsI GCATC 5 cut(s) 322, 385, 400, 883, 1213
BmuI ACTGGG 1 cut(s) 342
BpmI CTGGAG 1 cut(s) 824
Bpu10I CCTNAGC 1 cut(s) 813
Bpu14I TTCGAA 1 cut(s) 471
BpuEI CTTGAG 3 cut(s) 585, 724, 958
BpuMI CCSGG 2 cut(s) 737, 738
BsaHI GRCGYC 1 cut(s) 50
BsaJI CCNNGG 3 cut(s) 56, 736, 795
Bsc4I CCNNNNNNNGG 2 cut(s) 1183, 1213
Bse118I RCCGGY 1 cut(s) 317
Bse1I ACTGG 5 cut(s) 177, 348, 807, 1006, 1078
Bse21I CCTNAGG 1 cut(s) 846
Bse3DI GCAATG 1 cut(s) 1128
BseDI CCNNGG 3 cut(s) 56, 736, 795
BseGI GGATG 6 cut(s) 213, 343, 446, 765, 1222, 1238
BseLI CCNNNNNNNGG 2 cut(s) 1183, 1213
BseMI GCAATG 1 cut(s) 1128
BseMII CTCAG 2 cut(s) 827, 1136
BseNI ACTGG 5 cut(s) 177, 348, 807, 1006, 1078
BseRI GAGGAG 2 cut(s) 360, 962
BseXI GCAGC 2 cut(s) 637, 714
BshFI GGCC 7 cut(s) 321, 342, 735, 794, 818, 1150, 1176
BshNI GGYRCC 1 cut(s) 991
BsiHKAI GWGCWC 4 cut(s) 140, 497, 609, 710
BsiHKCI CYCGRG 2 cut(s) 55, 736
BsiSI CCGG 2 cut(s) 318, 737
BslFI GGGAC 1 cut(s) 37
BslI CCNNNNNNNGG 2 cut(s) 1183, 1213
BsmAI GTCTC 2 cut(s) 592, 1088
BsmFI GGGAC 1 cut(s) 37
BsnI GGCC 7 cut(s) 321, 342, 735, 794, 818, 1150, 1176
BsoBI CYCGRG 2 cut(s) 55, 736
Bsp119I TTCGAA 1 cut(s) 471
Bsp1286I GDGCHC 4 cut(s) 140, 497, 609, 710
Bsp1407I TGTACA 1 cut(s) 213
Bsp143I GATC 6 cut(s) 310, 403, 421, 451, 667, 1002
BspACI CCGC 3 cut(s) 205, 539, 914
BspANI GGCC 7 cut(s) 321, 342, 735, 794, 818, 1150, 1176
BspCNI CTCAG 2 cut(s) 826, 1137
BspLI GGNNCC 2 cut(s) 93, 993
BspMI ACCTGC 1 cut(s) 151
BspPI GGATC 4 cut(s) 411, 446, 662, 997
BspT104I TTCGAA 1 cut(s) 471
BspT107I GGYRCC 1 cut(s) 991
BsrDI GCAATG 1 cut(s) 1128
BsrFI RCCGGY 1 cut(s) 317
BsrGI TGTACA 1 cut(s) 213
BsrI ACTGG 5 cut(s) 177, 348, 807, 1006, 1078
BssAI RCCGGY 1 cut(s) 317
BssECI CCNNGG 3 cut(s) 56, 736, 795
BssMI GATC 6 cut(s) 310, 403, 421, 451, 667, 1002
BssNAI GTATAC 1 cut(s) 1039
BssNI GRCGYC 1 cut(s) 50
BssT1I CCWWGG 1 cut(s) 795
Bst1107I GTATAC 1 cut(s) 1039
Bst4CI ACNGT 2 cut(s) 101, 357
Bst6I CTCTTC 1 cut(s) 468
BstACI GRCGYC 1 cut(s) 50
BstAUI TGTACA 1 cut(s) 213
BstBI TTCGAA 1 cut(s) 471
BstC8I GCNNGC 4 cut(s) 319, 623, 661, 1013
BstDEI CTNAG 4 cut(s) 813, 846, 1145, 1299
BstEII GGTNACC 1 cut(s) 691
BstF5I GGATG 6 cut(s) 213, 343, 446, 765, 1222, 1238
BstH2I RGCGCY 1 cut(s) 631
BstHHI GCGC 2 cut(s) 502, 630
BstKTI GATC 6 cut(s) 313, 406, 424, 454, 670, 1005
BstMAI GTCTC 2 cut(s) 592, 1088
BstMBI GATC 6 cut(s) 310, 403, 421, 451, 667, 1002
BstMWI GCNNNNNNNGC 2 cut(s) 627, 1195
BstPI GGTNACC 1 cut(s) 691
BstSCI CCNGG 2 cut(s) 735, 736
BstV1I GCAGC 2 cut(s) 637, 714
BstX2I RGATCY 2 cut(s) 403, 451
BstXI CCANNNNNNTGG 3 cut(s) 151, 278, 802
BstYI RGATCY 2 cut(s) 403, 451
BstZ17I GTATAC 1 cut(s) 1039
Bsu36I CCTNAGG 1 cut(s) 846
BsuRI GGCC 7 cut(s) 321, 342, 735, 794, 818, 1150, 1176
BtsCI GGATG 6 cut(s) 213, 343, 446, 765, 1222, 1238
BtsIMutI CAGTG 2 cut(s) 800, 1013
BveI ACCTGC 1 cut(s) 151
Cac8I GCNNGC 4 cut(s) 319, 623, 661, 1013
CfoI GCGC 2 cut(s) 502, 630
Cfr10I RCCGGY 1 cut(s) 317
Cfr13I GGNCC 3 cut(s) 289, 734, 1106
Cfr9I CCCGGG 1 cut(s) 736
CseI GACGC 1 cut(s) 39
Csp6I GTAC 3 cut(s) 69, 214, 445
CviAII CATG 7 cut(s) 314, 519, 524, 731, 820, 833, 1212
CviQI GTAC 3 cut(s) 69, 214, 445
DdeI CTNAG 4 cut(s) 813, 846, 1145, 1299
DpnI GATC 6 cut(s) 312, 405, 423, 453, 669, 1004
DpnII GATC 6 cut(s) 310, 403, 421, 451, 667, 1002
DraI TTTAAA 1 cut(s) 45
EaeI YGGCCR 3 cut(s) 340, 792, 1174
Eam1104I CTCTTC 1 cut(s) 468
EarI CTCTTC 1 cut(s) 468
Ecl136II GAGCTC 2 cut(s) 607, 708
Eco130I CCWWGG 1 cut(s) 795
Eco24I GRGCYC 2 cut(s) 609, 710
Eco47I GGWCC 2 cut(s) 289, 1106
Eco53kI GAGCTC 2 cut(s) 607, 708
Eco57I CTGAAG 1 cut(s) 1286
Eco81I CCTNAGG 1 cut(s) 846
Eco88I CYCGRG 2 cut(s) 55, 736
Eco91I GGTNACC 1 cut(s) 691
EcoICRI GAGCTC 2 cut(s) 607, 708
EcoO65I GGTNACC 1 cut(s) 691
EcoRI GAATTC 1 cut(s) 467
EcoT14I CCWWGG 1 cut(s) 795
EcoT22I ATGCAT 1 cut(s) 551
EcoT38I GRGCYC 2 cut(s) 609, 710
ErhI CCWWGG 1 cut(s) 795
FaeI CATG 7 cut(s) 317, 522, 527, 734, 823, 836, 1215
FaqI GGGAC 1 cut(s) 37
FatI CATG 7 cut(s) 313, 518, 523, 730, 819, 832, 1211
FauNDI CATATG 1 cut(s) 979
FbaI TGATCA 1 cut(s) 421
FblI GTMKAC 3 cut(s) 259, 1038, 1290
Fnu4HI GCNGC 2 cut(s) 626, 728
FokI GGATG 6 cut(s) 220, 330, 453, 772, 1225, 1229
FriOI GRGCYC 2 cut(s) 609, 710
Fsp4HI GCNGC 2 cut(s) 626, 728
FspBI CTAG 2 cut(s) 407, 873
GlaI GCGC 2 cut(s) 501, 629
GluI GCNGC 2 cut(s) 626, 728
GsuI CTGGAG 1 cut(s) 824
HaeII RGCGCY 1 cut(s) 631
HaeIII GGCC 7 cut(s) 321, 342, 735, 794, 818, 1150, 1176
HapII CCGG 2 cut(s) 318, 737
HgaI GACGC 1 cut(s) 39
HhaI GCGC 2 cut(s) 502, 630
Hin1I GRCGYC 1 cut(s) 50
Hin1II CATG 7 cut(s) 317, 522, 527, 734, 823, 836, 1215
Hin6I GCGC 2 cut(s) 500, 628
HinP1I GCGC 2 cut(s) 500, 628
HincII GTYRAC 2 cut(s) 121, 328
HindII GTYRAC 2 cut(s) 121, 328
HindIII AAGCTT 1 cut(s) 1271
HinfI GANTC 3 cut(s) 38, 113, 569
HpaI GTTAAC 1 cut(s) 328
HpaII CCGG 2 cut(s) 318, 737
HphI GGTGA 3 cut(s) 553, 703, 1012
Hpy166II GTNNAC 7 cut(s) 121, 260, 328, 353, 1039, 1142, 1291
Hpy188I TCNGA 5 cut(s) 27, 403, 417, 1235, 1266
Hpy188III TCNNGA 9 cut(s) 110, 407, 455, 602, 703, 860, 937, 1048, 1160
Hpy8I GTNNAC 7 cut(s) 121, 260, 328, 353, 1039, 1142, 1291
Hpy99I CGWCG 1 cut(s) 1172
HpyAV CCTTC 1 cut(s) 408
HpyCH4III ACNGT 2 cut(s) 101, 357
HpyCH4IV ACGT 2 cut(s) 123, 614
HpyCH4V TGCA 7 cut(s) 376, 549, 621, 730, 823, 1121, 1260
HpyF10VI GCNNNNNNNGC 2 cut(s) 627, 1195
HpyF3I CTNAG 4 cut(s) 813, 846, 1145, 1299
HpySE526I ACGT 2 cut(s) 123, 614
Hsp92I GRCGYC 1 cut(s) 50
Hsp92II CATG 7 cut(s) 317, 522, 527, 734, 823, 836, 1215
HspAI GCGC 2 cut(s) 500, 628
KroI GCCGGC 1 cut(s) 317
KroNI GCCGGC 1 cut(s) 319
Ksp22I TGATCA 1 cut(s) 421
KspAI GTTAAC 1 cut(s) 328
Kzo9I GATC 6 cut(s) 310, 403, 421, 451, 667, 1002
LmnI GCTCC 4 cut(s) 91, 492, 713, 1186
Lsp1109I GCAGC 2 cut(s) 637, 714
LweI GCATC 5 cut(s) 322, 385, 400, 883, 1213
MaeI CTAG 2 cut(s) 407, 873
MaeII ACGT 2 cut(s) 123, 614
MaeIII GTNAC 5 cut(s) 74, 691, 860, 899, 923
MalI GATC 6 cut(s) 312, 405, 423, 453, 669, 1004
MboI GATC 6 cut(s) 310, 403, 421, 451, 667, 1002
MboII GAAGA 6 cut(s) 40, 485, 490, 845, 874, 1279
MflI RGATCY 2 cut(s) 403, 451
MhlI GDGCHC 4 cut(s) 140, 497, 609, 710
MlsI TGGCCA 3 cut(s) 342, 794, 1176
MluCI AATT 4 cut(s) 224, 467, 1078, 1191
MluNI TGGCCA 3 cut(s) 342, 794, 1176
MlyI GAGTC 2 cut(s) 47, 122
MmeI TCCRAC 2 cut(s) 132, 597
Mox20I TGGCCA 3 cut(s) 342, 794, 1176
Mph1103I ATGCAT 1 cut(s) 551
MroNI GCCGGC 1 cut(s) 317
MscI TGGCCA 3 cut(s) 342, 794, 1176
MseI TTAA 2 cut(s) 44, 327
MslI CAYNNNNRTG 1 cut(s) 636
Msp20I TGGCCA 3 cut(s) 342, 794, 1176
MspI CCGG 2 cut(s) 318, 737
MspR9I CCNGG 2 cut(s) 737, 738
MwoI GCNNNNNNNGC 2 cut(s) 627, 1195
NaeI GCCGGC 1 cut(s) 319
NciI CCSGG 2 cut(s) 737, 738
NdeI CATATG 1 cut(s) 979
NdeII GATC 6 cut(s) 310, 403, 421, 451, 667, 1002
NgoMIV GCCGGC 1 cut(s) 317
NlaIII CATG 7 cut(s) 317, 522, 527, 734, 823, 836, 1215
NlaIV GGNNCC 2 cut(s) 93, 993
NmuCI GTSAC 4 cut(s) 691, 860, 899, 923
NsiI ATGCAT 1 cut(s) 551
NspV TTCGAA 1 cut(s) 471
PaqCI CACCTGC 1 cut(s) 151
PdiI GCCGGC 1 cut(s) 319
PfeI GAWTC 1 cut(s) 569
PkrI GCNGC 2 cut(s) 627, 729
PleI GAGTC 2 cut(s) 46, 121
PpsI GAGTC 2 cut(s) 46, 121
PsiI TTATAA 1 cut(s) 1020
Psp124BI GAGCTC 2 cut(s) 609, 710
Psp1406I AACGTT 1 cut(s) 614
PspEI GGTNACC 1 cut(s) 691
PspN4I GGNNCC 2 cut(s) 93, 993
PspPI GGNCC 3 cut(s) 289, 734, 1106
PsuI RGATCY 2 cut(s) 403, 451
RsaI GTAC 3 cut(s) 70, 215, 446
RsaNI GTAC 3 cut(s) 69, 214, 445
RseI CAYNNNNRTG 1 cut(s) 636
SacI GAGCTC 2 cut(s) 609, 710
SaqAI TTAA 2 cut(s) 44, 327
SatI GCNGC 2 cut(s) 626, 728
Sau3AI GATC 6 cut(s) 310, 403, 421, 451, 667, 1002
Sau96I GGNCC 3 cut(s) 289, 734, 1106
SchI GAGTC 2 cut(s) 47, 122
ScrFI CCNGG 2 cut(s) 737, 738
SduI GDGCHC 4 cut(s) 140, 497, 609, 710
SfaNI GCATC 5 cut(s) 322, 385, 400, 883, 1213
SfuI TTCGAA 1 cut(s) 471
SinI GGWCC 2 cut(s) 289, 1106
SmaI CCCGGG 1 cut(s) 738
SmiMI CAYNNNNRTG 1 cut(s) 636
SmlI CTYRAG 3 cut(s) 600, 703, 937
SmoI CTYRAG 3 cut(s) 600, 703, 937
Sse9I AATT 4 cut(s) 224, 467, 1078, 1191
SsiI CCGC 3 cut(s) 205, 539, 914
SspMI CTAG 2 cut(s) 407, 873
SstI GAGCTC 2 cut(s) 609, 710
StyD4I CCNGG 2 cut(s) 735, 736
StyI CCWWGG 1 cut(s) 795
TaaI ACNGT 2 cut(s) 101, 357
TaiI ACGT 2 cut(s) 126, 617
TaqI TCGA 4 cut(s) 109, 131, 471, 609
TaqII GACCGA 2 cut(s) 710, 911
TasI AATT 4 cut(s) 224, 467, 1078, 1191
TatI WGTACW 1 cut(s) 213
TfiI GAWTC 1 cut(s) 569
Tru1I TTAA 2 cut(s) 44, 327
Tru9I TTAA 2 cut(s) 44, 327
TscAI CASTG 2 cut(s) 807, 1013
TseFI GTSAC 4 cut(s) 691, 860, 899, 923
TseI GCWGC 2 cut(s) 625, 727
Tsp45I GTSAC 4 cut(s) 691, 860, 899, 923
TspDTI ATGAA 5 cut(s) 17, 635, 845, 849, 1200
TspMI CCCGGG 1 cut(s) 736
TspRI CASTG 2 cut(s) 807, 1013
VpaK11BI GGWCC 2 cut(s) 289, 1106
XapI RAATTY 1 cut(s) 467
XbaI TCTAGA 1 cut(s) 406
XmaI CCCGGG 1 cut(s) 736
XmiI GTMKAC 3 cut(s) 259, 1038, 1290
XspI CTAG 2 cut(s) 407, 873
Zsp2I ATGCAT 1 cut(s) 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.