Rh6CG521200
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0014670
Physical Location & Seq
Forward (+)
67677915 .. 67680543
2629 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG521200.1

Sequence Viewer

Length: 1170 bp
ATGAACAAATTTCAAGGGATATTTCAGAAGAAAAAGGGGTCTCCAACGGCGTCTCCGAGAATTGATCCAAGTGCAGCGAAGCAAGTAACGCCTACTCATTACACGCCGGCCACTGTGGCAGCTGTGGAGCCGCACGGTTGTTGTCGAGATTCGGTTAACGTTGTTTCGATGAGCCAGTCTACCAAGAAGTTGGAACAGCAGGTGGTAGAACTGGAAAAGAAATTAGCAAAGGAGAAAGAGCTTCGGATGATGTACAGAAAGAAATTGGAAAGAACACAAGATTATTTGAGGTATTGTCTACAAGTAGCCCAAGAAAATGGGTTTTTGGACCTTATACTCCATAACAAAGATCATGCCGACACCGTTAACAATGCCACTGGTAATACCAGTCCTCAATTGCCAACTACTCCTCTGCACCAGCACCCGCACTACCCACCTTCTGATCTAGATGCTCTCATTGATCAAGCCAAGCTAAATGGATGGTTCATAAATCCAGATGAGATTGAATTTGAAGAGAAGATAGGGCAGGGGAGCACAGCACAGATATACAAAGCAACATGGCATGGACTTGATGTCGCGGTGAAATGCATAGAACCTGAATTCTTTGAATCAAACGAGAATGGTGTCTCATTTTTCGCTCAAGAGCTCGATACGTTGTGTAGGCAGCGTCATCGGTACGTGCTTCATCTAATGGGGGCGTGCCTTGATCCTCCTAACTATGCTTGGGTGGTGACCGAGTTCTTGAGCTCGACATTGAAAGAGTGGCTGCACGGCCCTGGAAATAGGCAAAAAGAGAGGATGATTGAGCTTCCTCCATTGGAAGAGAGGGTGACCAGGGCATTGGAGATCGCTCAGGCCATGCAGTATCTCCACGAACAAAAGCCTAAGGTTCTTCATCGCGACCTAAAGCCTACCAACATCTTTTTGGATGATGCTGGTCACGTAAGAGTTGCAGATTTTGGTCACGCTCGGTTCTTGAGTGATGAGGAGATGGCACTAACAGGGGAAACAGGAACATATGTGTACATGGCGCCAGAGGTGATCCAATGCGAGCCTTACAATGAGAAATGTGATGTATACAGCTTCGGGATTATACTCAATGAGATTATAACTGGAAATTATCCGTATATTGAGACAGATTATGGTCCTACTATGGTACGTATTAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

389

Amino Acids

44.35

Weight (kDa)

5.7

Isoelectric Point (pI)

40.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 168 - 379 9e-38 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 169 - 377 1.2e-42 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014670)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g31250
malus_domestica MD08G1069800.v1.1 MD15G1052200.v1.1
prunus_persica Prupe.1G406200_v2.0.a1 Prupe.1G406200_v2.0.a1
pyrus_communis pycom15g05000
rosa_chinensis RchiOBHm_Chr6g0311551
rosa_laevigata RLG00000010394
rosa_multiflora Rmu_co8269907.1_g000001 Rmu_sc0007556.1_g000001
rosa_roxburghii Rroxscaffold_7G00157500
rosa_rugosa Rorug06G0389700
rosa_samantha Rh6AG504600 Rh6BG515300 Rh6CG521200 Rh6DG507100
rosa_wichuraiana Rw6G043940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1109
AarI CACCTGC 1 cut(s) 190
Acc36I ACCTGC 1 cut(s) 190
AccB1I GGYRCC 1 cut(s) 1030
AccI GTMKAC 3 cut(s) 179, 298, 1077
AccII CGCG 2 cut(s) 578, 900
AciI CCGC 3 cut(s) 131, 425, 578
AclI AACGTT 1 cut(s) 159
AclWI GGATC 3 cut(s) 59, 701, 1036
AcoI YGGCCR 1 cut(s) 108
AcsI RAATTY 3 cut(s) 8, 506, 599
AcyI GRCGYC 2 cut(s) 50, 1031
AfaI GTAC 4 cut(s) 254, 677, 1025, 1158
AgsI TTSAA 5 cut(s) 14, 506, 512, 608, 757
AhdI GACNNNNNGTC 1 cut(s) 572
AjnI CCWGG 2 cut(s) 775, 833
AluBI AGCT 7 cut(s) 122, 241, 472, 646, 747, 808, 1083
AluI AGCT 7 cut(s) 122, 241, 472, 646, 747, 808, 1083
Alw21I GWGCWC 3 cut(s) 536, 648, 749
Alw26I GTCTC 4 cut(s) 45, 57, 631, 1127
AlwI GGATC 3 cut(s) 59, 701, 1036
AoxI GGCC 3 cut(s) 108, 772, 855
ApeKI GCWGC 4 cut(s) 74, 119, 664, 766
ApoI RAATTY 3 cut(s) 8, 506, 599
AspLEI GCGC 1 cut(s) 1033
AspS9I GGNCC 3 cut(s) 328, 773, 1145
AsuHPI GGTGA 4 cut(s) 592, 742, 841, 1051
AvaII GGWCC 2 cut(s) 328, 1145
AxyI CCTNAGG 1 cut(s) 885
BanI GGYRCC 1 cut(s) 1030
BanII GRGCYC 2 cut(s) 648, 749
Bbv12I GWGCWC 3 cut(s) 536, 648, 749
BbvI GCAGC 4 cut(s) 86, 131, 676, 753
BccI CCATC 2 cut(s) 474, 985
BceAI ACGGC 2 cut(s) 63, 787
BcgI CGANNNNNNTGC 2 cut(s) 653, 687
BciT130I CCWGG 2 cut(s) 777, 835
BclI TGATCA 1 cut(s) 460
BcoDI GTCTC 4 cut(s) 45, 57, 631, 1127
BfaI CTAG 1 cut(s) 446
BfoI RGCGCY 1 cut(s) 1034
BfuAI ACCTGC 1 cut(s) 190
BglI GCCNNNNNGGC 1 cut(s) 116
BisI GCNGC 5 cut(s) 75, 120, 131, 665, 767
BlsI GCNGC 5 cut(s) 76, 121, 132, 666, 768
Bme1390I CCNGG 2 cut(s) 777, 835
Bme18I GGWCC 2 cut(s) 328, 1145
BmeRI GACNNNNNGTC 1 cut(s) 572
BmgT120I GGNCC 3 cut(s) 328, 773, 1145
BmiI GGNNCC 2 cut(s) 129, 1032
BmrFI CCNGG 2 cut(s) 777, 835
BmsI GCATC 2 cut(s) 439, 922
Bpu10I CCTNAGC 1 cut(s) 852
BpuEI CTTGAG 3 cut(s) 624, 763, 997
BsaAI YACGTR 3 cut(s) 679, 943, 1160
BsaHI GRCGYC 2 cut(s) 50, 1031
BsaI GGTCTC 1 cut(s) 45
BsaJI CCNNGG 2 cut(s) 775, 834
BsaXI ACNNNNNCTCC 2 cut(s) 37, 67
Bse118I RCCGGY 1 cut(s) 106
Bse1I ACTGG 5 cut(s) 175, 216, 382, 387, 1117
Bse21I CCTNAGG 1 cut(s) 885
BseBI CCWGG 2 cut(s) 777, 835
BseDI CCNNGG 2 cut(s) 775, 834
BseGI GGATG 4 cut(s) 252, 485, 804, 934
BseMII CTCAG 1 cut(s) 866
BseNI ACTGG 5 cut(s) 175, 216, 382, 387, 1117
BseRI GAGGAG 2 cut(s) 399, 1001
BseXI GCAGC 4 cut(s) 86, 131, 676, 753
BsgI GTGCAG 3 cut(s) 93, 398, 752
Bsh1236I CGCG 2 cut(s) 578, 900
BshFI GGCC 3 cut(s) 110, 774, 857
BshNI GGYRCC 1 cut(s) 1030
BsiHKAI GWGCWC 3 cut(s) 536, 648, 749
BsiSI CCGG 1 cut(s) 107
BsmAI GTCTC 4 cut(s) 45, 57, 631, 1127
BsmBI CGTCTC 1 cut(s) 57
BsnI GGCC 3 cut(s) 110, 774, 857
Bso31I GGTCTC 1 cut(s) 45
Bsp1286I GDGCHC 3 cut(s) 536, 648, 749
Bsp1407I TGTACA 2 cut(s) 252, 1023
Bsp143I GATC 7 cut(s) 64, 349, 442, 460, 706, 846, 1041
Bsp68I TCGCGA 1 cut(s) 900
BspACI CCGC 3 cut(s) 131, 425, 578
BspANI GGCC 3 cut(s) 110, 774, 857
BspCNI CTCAG 1 cut(s) 865
BspFNI CGCG 2 cut(s) 578, 900
BspLI GGNNCC 2 cut(s) 129, 1032
BspMI ACCTGC 1 cut(s) 190
BspPI GGATC 3 cut(s) 59, 701, 1036
BspT107I GGYRCC 1 cut(s) 1030
BspTNI GGTCTC 1 cut(s) 45
BsrFI RCCGGY 1 cut(s) 106
BsrGI TGTACA 2 cut(s) 252, 1023
BsrI ACTGG 5 cut(s) 175, 216, 382, 387, 1117
BssAI RCCGGY 1 cut(s) 106
BssECI CCNNGG 2 cut(s) 775, 834
BssMI GATC 7 cut(s) 64, 349, 442, 460, 706, 846, 1041
BssNAI GTATAC 1 cut(s) 1078
BssNI GRCGYC 2 cut(s) 50, 1031
Bst1107I GTATAC 1 cut(s) 1078
Bst2UI CCWGG 2 cut(s) 777, 835
Bst4CI ACNGT 3 cut(s) 115, 137, 364
Bst6I CTCTTC 2 cut(s) 507, 816
BstACI GRCGYC 2 cut(s) 50, 1031
BstAUI TGTACA 2 cut(s) 252, 1023
BstBAI YACGTR 3 cut(s) 679, 943, 1160
BstC8I GCNNGC 3 cut(s) 108, 700, 1052
BstDEI CTNAG 2 cut(s) 852, 885
BstEII GGTNACC 2 cut(s) 730, 829
BstF5I GGATG 4 cut(s) 252, 485, 804, 934
BstFNI CGCG 2 cut(s) 578, 900
BstH2I RGCGCY 1 cut(s) 1034
BstHHI GCGC 1 cut(s) 1033
BstKTI GATC 7 cut(s) 67, 352, 445, 463, 709, 849, 1044
BstMAI GTCTC 4 cut(s) 45, 57, 631, 1127
BstMBI GATC 7 cut(s) 64, 349, 442, 460, 706, 846, 1041
BstMWI GCNNNNNNNGC 2 cut(s) 88, 116
BstNI CCWGG 2 cut(s) 777, 835
BstPI GGTNACC 2 cut(s) 730, 829
BstSCI CCNGG 2 cut(s) 775, 833
BstSNI TACGTA 1 cut(s) 1160
BstUI CGCG 2 cut(s) 578, 900
BstV1I GCAGC 4 cut(s) 86, 131, 676, 753
BstXI CCANNNNNNTGG 3 cut(s) 190, 317, 841
BstZ17I GTATAC 1 cut(s) 1078
Bsu36I CCTNAGG 1 cut(s) 885
BsuRI GGCC 3 cut(s) 110, 774, 857
BtgZI GCGATG 1 cut(s) 881
BtsCI GGATG 4 cut(s) 252, 485, 804, 934
BtsIMutI CAGTG 2 cut(s) 111, 375
BtuMI TCGCGA 1 cut(s) 900
BveI ACCTGC 1 cut(s) 190
Cac8I GCNNGC 3 cut(s) 108, 700, 1052
CfoI GCGC 1 cut(s) 1033
Cfr10I RCCGGY 1 cut(s) 106
Cfr13I GGNCC 3 cut(s) 328, 773, 1145
CseI GACGC 2 cut(s) 39, 656
Csp6I GTAC 4 cut(s) 253, 676, 1024, 1157
CviAII CATG 5 cut(s) 353, 558, 563, 859, 1027
CviQI GTAC 4 cut(s) 253, 676, 1024, 1157
DdeI CTNAG 2 cut(s) 852, 885
DinI GGCGCC 1 cut(s) 1032
DpnI GATC 7 cut(s) 66, 351, 444, 462, 708, 848, 1043
DpnII GATC 7 cut(s) 64, 349, 442, 460, 706, 846, 1041
DriI GACNNNNNGTC 1 cut(s) 572
EaeI YGGCCR 1 cut(s) 108
Eam1104I CTCTTC 2 cut(s) 507, 816
Eam1105I GACNNNNNGTC 1 cut(s) 572
EarI CTCTTC 2 cut(s) 507, 816
Ecl136II GAGCTC 2 cut(s) 646, 747
Eco105I TACGTA 1 cut(s) 1160
Eco24I GRGCYC 2 cut(s) 648, 749
Eco31I GGTCTC 1 cut(s) 45
Eco47I GGWCC 2 cut(s) 328, 1145
Eco53kI GAGCTC 2 cut(s) 646, 747
Eco81I CCTNAGG 1 cut(s) 885
Eco91I GGTNACC 2 cut(s) 730, 829
EcoICRI GAGCTC 2 cut(s) 646, 747
EcoO65I GGTNACC 2 cut(s) 730, 829
EcoRI GAATTC 1 cut(s) 599
EcoRII CCWGG 2 cut(s) 775, 833
EcoT22I ATGCAT 1 cut(s) 590
EcoT38I GRGCYC 2 cut(s) 648, 749
EgeI GGCGCC 1 cut(s) 1032
EheI GGCGCC 1 cut(s) 1032
Esp3I CGTCTC 1 cut(s) 57
FaeI CATG 5 cut(s) 356, 561, 566, 862, 1030
FatI CATG 5 cut(s) 352, 557, 562, 858, 1026
FauI CCCGC 1 cut(s) 432
FauNDI CATATG 1 cut(s) 1018
FbaI TGATCA 1 cut(s) 460
FblI GTMKAC 3 cut(s) 179, 298, 1077
Fnu4HI GCNGC 5 cut(s) 75, 120, 131, 665, 767
FokI GGATG 4 cut(s) 259, 492, 811, 941
FriOI GRGCYC 2 cut(s) 648, 749
Fsp4HI GCNGC 5 cut(s) 75, 120, 131, 665, 767
FspBI CTAG 1 cut(s) 446
GlaI GCGC 1 cut(s) 1032
GluI GCNGC 5 cut(s) 75, 120, 131, 665, 767
HaeII RGCGCY 1 cut(s) 1034
HaeIII GGCC 3 cut(s) 110, 774, 857
HapII CCGG 1 cut(s) 107
HgaI GACGC 2 cut(s) 39, 656
HhaI GCGC 1 cut(s) 1033
Hin1I GRCGYC 2 cut(s) 50, 1031
Hin1II CATG 5 cut(s) 356, 561, 566, 862, 1030
Hin6I GCGC 1 cut(s) 1031
HinP1I GCGC 1 cut(s) 1031
HincII GTYRAC 2 cut(s) 157, 367
HindII GTYRAC 2 cut(s) 157, 367
HinfI GANTC 2 cut(s) 149, 608
HpaI GTTAAC 2 cut(s) 157, 367
HpaII CCGG 1 cut(s) 107
HphI GGTGA 4 cut(s) 592, 742, 841, 1051
Hpy166II GTNNAC 6 cut(s) 157, 180, 299, 367, 1024, 1078
Hpy188I TCNGA 4 cut(s) 27, 57, 246, 442
Hpy188III TCNNGA 8 cut(s) 146, 446, 494, 641, 742, 899, 976, 1087
Hpy8I GTNNAC 6 cut(s) 157, 180, 299, 367, 1024, 1078
HpyAV CCTTC 1 cut(s) 447
HpyCH4III ACNGT 3 cut(s) 115, 137, 364
HpyCH4IV ACGT 5 cut(s) 159, 653, 678, 942, 1159
HpyCH4V TGCA 6 cut(s) 74, 415, 588, 769, 862, 953
HpyF10VI GCNNNNNNNGC 2 cut(s) 88, 116
HpyF3I CTNAG 2 cut(s) 852, 885
HpySE526I ACGT 5 cut(s) 159, 653, 678, 942, 1159
Hsp92I GRCGYC 2 cut(s) 50, 1031
Hsp92II CATG 5 cut(s) 356, 561, 566, 862, 1030
HspAI GCGC 1 cut(s) 1031
KasI GGCGCC 1 cut(s) 1030
KroI GCCGGC 1 cut(s) 106
KroNI GCCGGC 1 cut(s) 108
Ksp22I TGATCA 1 cut(s) 460
KspAI GTTAAC 2 cut(s) 157, 367
Kzo9I GATC 7 cut(s) 64, 349, 442, 460, 706, 846, 1041
LmnI GCTCC 2 cut(s) 127, 531
Lsp1109I GCAGC 4 cut(s) 86, 131, 676, 753
LweI GCATC 2 cut(s) 439, 922
MaeI CTAG 1 cut(s) 446
MaeII ACGT 5 cut(s) 159, 653, 678, 942, 1159
MaeIII GTNAC 5 cut(s) 85, 730, 829, 938, 962
MalI GATC 7 cut(s) 66, 351, 444, 462, 708, 848, 1043
MboI GATC 7 cut(s) 64, 349, 442, 460, 706, 846, 1041
MboII GAAGA 5 cut(s) 40, 524, 529, 833, 884
MfeI CAATTG 1 cut(s) 395
MhlI GDGCHC 3 cut(s) 536, 648, 749
MluCI AATT 8 cut(s) 8, 60, 221, 263, 395, 506, 599, 1117
Mly113I GGCGCC 1 cut(s) 1031
MmeI TCCRAC 2 cut(s) 68, 171
MnlI CCTC 9 cut(s) 282, 402, 420, 720, 789, 819, 822, 979, 1030
Mph1103I ATGCAT 1 cut(s) 590
MroNI GCCGGC 1 cut(s) 106
MseI TTAA 3 cut(s) 156, 366, 1168
MspA1I CMGCKG 1 cut(s) 122
MspI CCGG 1 cut(s) 107
MspR9I CCNGG 2 cut(s) 777, 835
MunI CAATTG 1 cut(s) 395
MvaI CCWGG 2 cut(s) 777, 835
MvnI CGCG 2 cut(s) 578, 900
MwoI GCNNNNNNNGC 2 cut(s) 88, 116
NaeI GCCGGC 1 cut(s) 108
NarI GGCGCC 1 cut(s) 1031
NdeI CATATG 1 cut(s) 1018
NdeII GATC 7 cut(s) 64, 349, 442, 460, 706, 846, 1041
NgoMIV GCCGGC 1 cut(s) 106
NlaIII CATG 5 cut(s) 356, 561, 566, 862, 1030
NlaIV GGNNCC 2 cut(s) 129, 1032
NmuCI GTSAC 4 cut(s) 730, 829, 938, 962
NruI TCGCGA 1 cut(s) 900
NsiI ATGCAT 1 cut(s) 590
PaqCI CACCTGC 1 cut(s) 190
PcsI WCGNNNNNNNCGW 1 cut(s) 53
PdiI GCCGGC 1 cut(s) 108
PfeI GAWTC 2 cut(s) 149, 608
PkrI GCNGC 5 cut(s) 76, 121, 132, 666, 768
PluTI GGCGCC 1 cut(s) 1034
Ppu21I YACGTR 3 cut(s) 679, 943, 1160
PsiI TTATAA 1 cut(s) 1109
Psp124BI GAGCTC 2 cut(s) 648, 749
Psp1406I AACGTT 1 cut(s) 159
Psp6I CCWGG 2 cut(s) 775, 833
PspEI GGTNACC 2 cut(s) 730, 829
PspGI CCWGG 2 cut(s) 775, 833
PspN4I GGNNCC 2 cut(s) 129, 1032
PspPI GGNCC 3 cut(s) 328, 773, 1145
PvuII CAGCTG 1 cut(s) 122
RruI TCGCGA 1 cut(s) 900
RsaI GTAC 4 cut(s) 254, 677, 1025, 1158
RsaNI GTAC 4 cut(s) 253, 676, 1024, 1157
SacI GAGCTC 2 cut(s) 648, 749
SaqAI TTAA 3 cut(s) 156, 366, 1168
SatI GCNGC 5 cut(s) 75, 120, 131, 665, 767
Sau3AI GATC 7 cut(s) 64, 349, 442, 460, 706, 846, 1041
Sau96I GGNCC 3 cut(s) 328, 773, 1145
ScrFI CCNGG 2 cut(s) 777, 835
SduI GDGCHC 3 cut(s) 536, 648, 749
SfaNI GCATC 2 cut(s) 439, 922
SfoI GGCGCC 1 cut(s) 1032
SinI GGWCC 2 cut(s) 328, 1145
SmlI CTYRAG 3 cut(s) 639, 742, 976
SmoI CTYRAG 3 cut(s) 639, 742, 976
SnaBI TACGTA 1 cut(s) 1160
Sse9I AATT 8 cut(s) 8, 60, 221, 263, 395, 506, 599, 1117
SsiI CCGC 3 cut(s) 131, 425, 578
SspDI GGCGCC 1 cut(s) 1030
SspMI CTAG 1 cut(s) 446
SstI GAGCTC 2 cut(s) 648, 749
StyD4I CCNGG 2 cut(s) 775, 833
TaaI ACNGT 3 cut(s) 115, 137, 364
TaiI ACGT 5 cut(s) 162, 656, 681, 945, 1162
TaqI TCGA 4 cut(s) 145, 167, 648, 749
TaqII GACCGA 1 cut(s) 749
TasI AATT 8 cut(s) 8, 60, 221, 263, 395, 506, 599, 1117
TatI WGTACW 2 cut(s) 252, 1023
TauI GCSGC 1 cut(s) 133
TfiI GAWTC 2 cut(s) 149, 608
Tru1I TTAA 3 cut(s) 156, 366, 1168
Tru9I TTAA 3 cut(s) 156, 366, 1168
TscAI CASTG 2 cut(s) 118, 382
TseFI GTSAC 4 cut(s) 730, 829, 938, 962
TseI GCWGC 4 cut(s) 74, 119, 664, 766
Tsp45I GTSAC 4 cut(s) 730, 829, 938, 962
TspDTI ATGAA 4 cut(s) 17, 475, 674, 884
TspGWI ACGGA 1 cut(s) 1113
TspRI CASTG 2 cut(s) 118, 382
VpaK11BI GGWCC 2 cut(s) 328, 1145
XapI RAATTY 3 cut(s) 8, 506, 599
XbaI TCTAGA 1 cut(s) 445
XcmI CCANNNNNNNNNTGG 1 cut(s) 922
XmiI GTMKAC 3 cut(s) 179, 298, 1077
XspI CTAG 1 cut(s) 446
Zsp2I ATGCAT 1 cut(s) 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.