pycom15g05000
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0014670
Physical Location & Seq
Reverse (-)
2997413 .. 2998507
1095 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g05000.2

Sequence Viewer

Length: 747 bp
ATGTACCCTGATTTCTTTGAAACGAACGACAATGGTGTCGCATTTTTCGCTCAAGAGCTTGACACCTTATGTAGGCAGCGACATCCATACATACTTCAGCTAATGGGTGCATGCCTCGATCCTCCTAACTATGCTTGGGTGGTGACGGAGTTCATGAGTACAACACTAAAGGAGTGGCTTCACGGCCCTGGAAATAGGCGAAAAGAGAGGATGATACCACTTCCTCCGCTCAAAGAGAGGGTCGCTAGGGCATTGGAGATTGCTCTAGCCATGCAGTATCTTCATGGACAAAAGCCTATGGTGATTCATCGTGATCTCAAGCCTAGCAACATATTTTTGGACGATGCTATGCATGTACGAGTTGCGGATTTTGGTCATGCTCGGTTCTTGAGTGATGAAGAGAAGGCACTCACTGGCGAAACAGGAACTTATGTGTACATGGCACCCGAGGTCATCCAATGCGAGCCTTATAACGAAAAATGTGACGTATACAGTTTTGGGATTTTATTGAATGAGCTTATAACTGGAGATTATCCTTACATTGAGAAAGATTACGGTCCCATTAAGATTGCGATGGAAGTTGTGGAGGACAACCTAAGGCCAAAACTGGCTGATGATCATGATGGGCAACTTGGGGAGCTAATTGACCTAATTTGCCTTTCATGGGATGCAGATGCGTCAGTGAGACCATCCTTTGCCGCCATTACAAGTAGTTTGAAGGCTCTTCAAAACAGATTGTTTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

249

Amino Acids

28.37

Weight (kDa)

4.94

Isoelectric Point (pI)

36.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014670)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g31250
malus_domestica MD08G1069800.v1.1 MD15G1052200.v1.1
prunus_persica Prupe.1G406200_v2.0.a1 Prupe.1G406200_v2.0.a1
pyrus_communis pycom15g05000
rosa_chinensis RchiOBHm_Chr6g0311551
rosa_laevigata RLG00000010394
rosa_multiflora Rmu_co8269907.1_g000001 Rmu_sc0007556.1_g000001
rosa_roxburghii Rroxscaffold_7G00157500
rosa_rugosa Rorug06G0389700
rosa_samantha Rh6AG504600 Rh6BG515300 Rh6CG521200 Rh6DG507100
rosa_wichuraiana Rw6G043940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 471, 521
AasI GACNNNNNNGTC 1 cut(s) 35
AccB1I GGYRCC 1 cut(s) 442
AccBSI CCGCTC 1 cut(s) 229
AccI GTMKAC 1 cut(s) 489
AciI CCGC 3 cut(s) 227, 365, 699
AclWI GGATC 1 cut(s) 113
AcuI CTGAAG 1 cut(s) 80
AfaI GTAC 4 cut(s) 5, 160, 357, 437
AfiI CCNNNNNNNGG 2 cut(s) 72, 664
AgsI TTSAA 4 cut(s) 20, 511, 718, 728
AjnI CCWGG 1 cut(s) 187
AluBI AGCT 4 cut(s) 58, 100, 517, 640
AluI AGCT 4 cut(s) 58, 100, 517, 640
Alw26I GTCTC 1 cut(s) 679
AlwI GGATC 1 cut(s) 113
Ama87I CYCGRG 1 cut(s) 446
AoxI GGCC 2 cut(s) 184, 599
ApeKI GCWGC 1 cut(s) 76
ArsI GACNNNNNNTTYG 2 cut(s) 225, 257
AspS9I GGNCC 2 cut(s) 185, 557
AsuHPI GGTGA 2 cut(s) 154, 313
AvaI CYCGRG 1 cut(s) 446
AvaII GGWCC 1 cut(s) 557
AxyI CCTNAGG 1 cut(s) 596
BanI GGYRCC 1 cut(s) 442
BbvI GCAGC 1 cut(s) 88
BccI CCATC 3 cut(s) 568, 617, 697
BceAI ACGGC 1 cut(s) 199
BciT130I CCWGG 1 cut(s) 189
BclI TGATCA 1 cut(s) 616
BcoDI GTCTC 1 cut(s) 679
BfaI CTAG 3 cut(s) 246, 266, 324
BisI GCNGC 2 cut(s) 77, 699
BlsI GCNGC 2 cut(s) 78, 700
Bme1390I CCNGG 1 cut(s) 189
Bme18I GGWCC 1 cut(s) 557
BmeT110I CYCGRG 1 cut(s) 446
BmgT120I GGNCC 2 cut(s) 185, 557
BmiI GGNNCC 2 cut(s) 444, 559
BmrFI CCNGG 1 cut(s) 189
BmsI GCATC 3 cut(s) 334, 658, 664
BpmI CTGGAG 1 cut(s) 546
BpuEI CTTGAG 3 cut(s) 36, 302, 409
BsaI GGTCTC 1 cut(s) 679
BsaJI CCNNGG 2 cut(s) 187, 447
Bsc4I CCNNNNNNNGG 2 cut(s) 72, 664
Bse1I ACTGG 3 cut(s) 418, 529, 612
Bse21I CCTNAGG 1 cut(s) 596
BseBI CCWGG 1 cut(s) 189
BseDI CCNNGG 2 cut(s) 187, 447
BseGI GGATG 5 cut(s) 82, 216, 453, 673, 689
BseLI CCNNNNNNNGG 2 cut(s) 72, 664
BseNI ACTGG 3 cut(s) 418, 529, 612
BseXI GCAGC 1 cut(s) 88
BshFI GGCC 2 cut(s) 186, 601
BshNI GGYRCC 1 cut(s) 442
BsiHKCI CYCGRG 1 cut(s) 446
BslFI GGGAC 1 cut(s) 543
BslI CCNNNNNNNGG 2 cut(s) 72, 664
BsmAI GTCTC 1 cut(s) 679
BsmFI GGGAC 1 cut(s) 543
BsnI GGCC 2 cut(s) 186, 601
Bso31I GGTCTC 1 cut(s) 679
BsoBI CYCGRG 1 cut(s) 446
Bsp1407I TGTACA 1 cut(s) 435
Bsp143I GATC 3 cut(s) 118, 313, 616
BspACI CCGC 3 cut(s) 227, 365, 699
BspANI GGCC 2 cut(s) 186, 601
BspHI TCATGA 2 cut(s) 153, 619
BspLI GGNNCC 2 cut(s) 444, 559
BspPI GGATC 1 cut(s) 113
BspQI GCTCTTC 1 cut(s) 729
BspT107I GGYRCC 1 cut(s) 442
BspTNI GGTCTC 1 cut(s) 679
BsrBI CCGCTC 1 cut(s) 229
BsrGI TGTACA 1 cut(s) 435
BsrI ACTGG 3 cut(s) 418, 529, 612
BssECI CCNNGG 2 cut(s) 187, 447
BssMI GATC 3 cut(s) 118, 313, 616
BssNAI GTATAC 1 cut(s) 490
Bst1107I GTATAC 1 cut(s) 490
Bst2UI CCWGG 1 cut(s) 189
Bst4CI ACNGT 2 cut(s) 494, 557
Bst6I CTCTTC 2 cut(s) 393, 729
BstAUI TGTACA 1 cut(s) 435
BstC8I GCNNGC 2 cut(s) 112, 464
BstDEI CTNAG 1 cut(s) 596
BstENI CCTNNNNNAGG 1 cut(s) 70
BstF5I GGATG 5 cut(s) 82, 216, 453, 673, 689
BstKTI GATC 3 cut(s) 121, 316, 619
BstMAI GTCTC 1 cut(s) 679
BstMBI GATC 3 cut(s) 118, 313, 616
BstMWI GCNNNNNNNGC 1 cut(s) 47
BstNI CCWGG 1 cut(s) 189
BstNSI RCATGY 2 cut(s) 114, 356
BstSCI CCNGG 1 cut(s) 187
BstV1I GCAGC 1 cut(s) 88
BstZ17I GTATAC 1 cut(s) 490
Bsu36I CCTNAGG 1 cut(s) 596
BsuRI GGCC 2 cut(s) 186, 601
BtgZI GCGATG 1 cut(s) 587
BtsCI GGATG 5 cut(s) 82, 216, 453, 673, 689
BtsIMutI CAGTG 2 cut(s) 411, 687
Cac8I GCNNGC 2 cut(s) 112, 464
CciI TCATGA 2 cut(s) 153, 619
Cfr13I GGNCC 2 cut(s) 185, 557
CseI GACGC 1 cut(s) 666
Csp6I GTAC 4 cut(s) 4, 159, 356, 436
CviAII CATG 9 cut(s) 111, 154, 271, 284, 353, 377, 439, 620, 663
CviQI GTAC 4 cut(s) 4, 159, 356, 436
DdeI CTNAG 1 cut(s) 596
DpnI GATC 3 cut(s) 120, 315, 618
DpnII GATC 3 cut(s) 118, 313, 616
DrdI GACNNNNNNGTC 1 cut(s) 35
DseDI GACNNNNNNGTC 1 cut(s) 35
Eam1104I CTCTTC 2 cut(s) 393, 729
EarI CTCTTC 2 cut(s) 393, 729
Eco31I GGTCTC 1 cut(s) 679
Eco47I GGWCC 1 cut(s) 557
Eco57I CTGAAG 1 cut(s) 80
Eco81I CCTNAGG 1 cut(s) 596
Eco88I CYCGRG 1 cut(s) 446
EcoNI CCTNNNNNAGG 1 cut(s) 70
EcoRII CCWGG 1 cut(s) 187
EcoT22I ATGCAT 1 cut(s) 354
FaeI CATG 9 cut(s) 114, 157, 274, 287, 356, 380, 442, 623, 666
FaqI GGGAC 1 cut(s) 543
FatI CATG 9 cut(s) 110, 153, 270, 283, 352, 376, 438, 619, 662
FbaI TGATCA 1 cut(s) 616
FblI GTMKAC 1 cut(s) 489
Fnu4HI GCNGC 2 cut(s) 77, 699
FokI GGATG 5 cut(s) 69, 223, 440, 676, 680
Fsp4HI GCNGC 2 cut(s) 77, 699
FspBI CTAG 3 cut(s) 246, 266, 324
GluI GCNGC 2 cut(s) 77, 699
GsuI CTGGAG 1 cut(s) 546
HaeIII GGCC 2 cut(s) 186, 601
HgaI GACGC 1 cut(s) 666
Hin1II CATG 9 cut(s) 114, 157, 274, 287, 356, 380, 442, 623, 666
HinfI GANTC 1 cut(s) 304
HphI GGTGA 2 cut(s) 154, 313
Hpy166II GTNNAC 2 cut(s) 436, 490
Hpy188III TCNNGA 5 cut(s) 53, 154, 311, 388, 620
Hpy8I GTNNAC 2 cut(s) 436, 490
HpyAV CCTTC 2 cut(s) 397, 712
HpyCH4III ACNGT 2 cut(s) 494, 557
HpyCH4IV ACGT 1 cut(s) 486
HpyCH4V TGCA 4 cut(s) 110, 274, 352, 671
HpyF10VI GCNNNNNNNGC 1 cut(s) 47
HpyF3I CTNAG 1 cut(s) 596
HpySE526I ACGT 1 cut(s) 486
Hsp92II CATG 9 cut(s) 114, 157, 274, 287, 356, 380, 442, 623, 666
Ksp22I TGATCA 1 cut(s) 616
Kzo9I GATC 3 cut(s) 118, 313, 616
LguI GCTCTTC 1 cut(s) 729
LmnI GCTCC 1 cut(s) 637
LpnPI CCDG 7 cut(s) 21, 174, 201, 399, 408, 510, 593
Lsp1109I GCAGC 1 cut(s) 88
LweI GCATC 3 cut(s) 334, 658, 664
MaeI CTAG 3 cut(s) 246, 266, 324
MaeII ACGT 1 cut(s) 486
MaeIII GTNAC 2 cut(s) 142, 482
MalI GATC 3 cut(s) 120, 315, 618
MbiI CCGCTC 1 cut(s) 229
MboI GATC 3 cut(s) 118, 313, 616
MboII GAAGA 3 cut(s) 272, 410, 716
MluCI AATT 2 cut(s) 642, 651
MnlI CCTC 7 cut(s) 125, 132, 201, 231, 234, 442, 580
Mph1103I ATGCAT 1 cut(s) 354
MseI TTAA 1 cut(s) 564
MspR9I CCNGG 1 cut(s) 189
MvaI CCWGG 1 cut(s) 189
MwoI GCNNNNNNNGC 1 cut(s) 47
NdeII GATC 3 cut(s) 118, 313, 616
NlaIII CATG 9 cut(s) 114, 157, 274, 287, 356, 380, 442, 623, 666
NlaIV GGNNCC 2 cut(s) 444, 559
NmuCI GTSAC 2 cut(s) 142, 482
NsiI ATGCAT 1 cut(s) 354
NspI RCATGY 2 cut(s) 114, 356
PaeI GCATGC 1 cut(s) 114
PagI TCATGA 2 cut(s) 153, 619
PciSI GCTCTTC 1 cut(s) 729
PfeI GAWTC 1 cut(s) 304
PkrI GCNGC 2 cut(s) 78, 700
PsiI TTATAA 2 cut(s) 471, 521
Psp6I CCWGG 1 cut(s) 187
PspGI CCWGG 1 cut(s) 187
PspN4I GGNNCC 2 cut(s) 444, 559
PspPI GGNCC 2 cut(s) 185, 557
RsaI GTAC 4 cut(s) 5, 160, 357, 437
RsaNI GTAC 4 cut(s) 4, 159, 356, 436
SapI GCTCTTC 1 cut(s) 729
SaqAI TTAA 1 cut(s) 564
SatI GCNGC 2 cut(s) 77, 699
Sau3AI GATC 3 cut(s) 118, 313, 616
Sau96I GGNCC 2 cut(s) 185, 557
ScrFI CCNGG 1 cut(s) 189
SetI ASST 9 cut(s) 60, 68, 102, 453, 489, 519, 597, 642, 651
SfaNI GCATC 3 cut(s) 334, 658, 664
SinI GGWCC 1 cut(s) 557
SmlI CTYRAG 3 cut(s) 51, 317, 388
SmoI CTYRAG 3 cut(s) 51, 317, 388
SphI GCATGC 1 cut(s) 114
Sse9I AATT 2 cut(s) 642, 651
SsiI CCGC 3 cut(s) 227, 365, 699
SspMI CTAG 3 cut(s) 246, 266, 324
StyD4I CCNGG 1 cut(s) 187
TaaI ACNGT 2 cut(s) 494, 557
TaiI ACGT 1 cut(s) 489
TaqI TCGA 1 cut(s) 117
TasI AATT 2 cut(s) 642, 651
TatI WGTACW 2 cut(s) 158, 435
TauI GCSGC 1 cut(s) 701
TfiI GAWTC 1 cut(s) 304
Tru1I TTAA 1 cut(s) 564
Tru9I TTAA 1 cut(s) 564
TscAI CASTG 2 cut(s) 418, 687
TseFI GTSAC 2 cut(s) 142, 482
TseI GCWGC 1 cut(s) 76
Tsp45I GTSAC 2 cut(s) 142, 482
TspDTI ATGAA 5 cut(s) 142, 272, 296, 411, 651
TspGWI ACGGA 1 cut(s) 161
TspRI CASTG 2 cut(s) 418, 687
VpaK11BI GGWCC 1 cut(s) 557
XagI CCTNNNNNAGG 1 cut(s) 70
XceI RCATGY 2 cut(s) 114, 356
XmiI GTMKAC 1 cut(s) 489
XspI CTAG 3 cut(s) 246, 266, 324
Zsp2I ATGCAT 1 cut(s) 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.