Rh6AG504600
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0014670
Physical Location & Seq
Forward (+)
67698537 .. 67701210
2674 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG504600.1

Sequence Viewer

Length: 1224 bp
ATGAACAAATTTCAAGGGATATTTCAGAAGAAAAAGGGGTCTCCAACGGCGTTGCCGAGAATTGATCCTAGCGCAGCGAAGCAAGTAACGCCTACTCATTACACGCCGGCCACTGTGGCAGCTGTGGAGCCGCACGGTTGTTGTCGAGATTCGGTCAACGTTTTTTCGATGAGCCAGTCTACCAAGAAGTTGGAACAGCAGGTGGCAGAACTGGAAAAGAAATTAGCAAAGGAGAAAGAGCTTCGGATGATGTACATAAAGAAATTGGAAAGAACACAAGATTATTTGAGGTATTGTCTACAAGTAGCCCAAGAAAATGGGTTTTTGGACCTTGTACTCCATAACAAAGATCATGCCGACACTGTTAACAATGCCACTGGTAATACCAGTCCTCAATTGCCAACTACTCCTCTGCACCAGCACCACCCACCTTCTGATCTAGATGCTCTCATCGATCAAGCCAAGCTAAATGGATGGTTCATAAATACAGATGAGATTGAATTTGAAGAGAAGATAGGGCAGGGGAGCACAGCACAGATATACAAAGCAACATGGCATGGACTTGATGTCGCGGTGAAATGCATAGAACCTGAATTCTTTGAATCAAACGAGAATGGTGTCTCATTTTTCGCTCAAGAGCTCGATACGTTATGTAGGCAGCGTCATCGGTACGTGCTTCATCTAATGGGGGCGTGCCTTGATCCTCCTAACTATGCTTGGGTGGTGACCGAGTTCTTGAGCTCGACATTGAAAGAGTGGCTGCACGGCCCTGGAAATAGGCAAAAAGAGAGGATGATTGAGCTTCCTCCATTGGAAGAGAGGGTGGCCAGGGCATTGGAGATCGCTCAGGCCATGCAGTATCTCCACGAACAAAAGCCTAAGGTTCTTCATCGCGACCTAAAGCCTAGCAACATCTTTTTGGATGACGCTGGTCACGTAAGAGTTGCAGATTTTGGTCACGCTCGGTTCTTGAGTGATGAGGAGATGGCACTAACAGGGGAAACAGGAACATATGTGTACATGGCGCCAGAGGTGATCCAATGCGAGCCTTACAATGAGAAATGTGATGTATACAGCTTCGGGATTATACTCAATGAGATTATAACTGGAAATTATCCATATATTGAGACAGATTATGGTCCTACTATGGTACGTATTAGTACGTTAAACACCTCTTCCTCAAGTCAAGCAAATATGAATGTTAAACAAAAGCCTCACCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

407

Amino Acids

46.21

Weight (kDa)

5.83

Isoelectric Point (pI)

39.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 166 - 378 6.8e-38 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 167 - 377 3.1e-43 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014670)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g31250
malus_domestica MD08G1069800.v1.1 MD15G1052200.v1.1
prunus_persica Prupe.1G406200_v2.0.a1 Prupe.1G406200_v2.0.a1
pyrus_communis pycom15g05000
rosa_chinensis RchiOBHm_Chr6g0311551
rosa_laevigata RLG00000010394
rosa_multiflora Rmu_co8269907.1_g000001 Rmu_sc0007556.1_g000001
rosa_roxburghii Rroxscaffold_7G00157500
rosa_rugosa Rorug06G0389700
rosa_samantha Rh6AG504600 Rh6BG515300 Rh6CG521200 Rh6DG507100
rosa_wichuraiana Rw6G043940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1103
AarI CACCTGC 1 cut(s) 190
Acc36I ACCTGC 1 cut(s) 190
AccB1I GGYRCC 1 cut(s) 1024
AccI GTMKAC 3 cut(s) 179, 298, 1071
AccII CGCG 2 cut(s) 572, 894
AciI CCGC 2 cut(s) 131, 572
AclI AACGTT 1 cut(s) 159
AclWI GGATC 3 cut(s) 59, 695, 1030
AcoI YGGCCR 2 cut(s) 108, 825
AcsI RAATTY 3 cut(s) 8, 500, 593
AcyI GRCGYC 1 cut(s) 1025
AfaI GTAC 6 cut(s) 254, 336, 671, 1019, 1152, 1162
AgsI TTSAA 5 cut(s) 14, 500, 506, 602, 751
AhdI GACNNNNNGTC 1 cut(s) 566
AjnI CCWGG 2 cut(s) 769, 827
AluBI AGCT 7 cut(s) 122, 241, 466, 640, 741, 802, 1077
AluI AGCT 7 cut(s) 122, 241, 466, 640, 741, 802, 1077
Alw21I GWGCWC 3 cut(s) 530, 642, 743
Alw26I GTCTC 3 cut(s) 45, 625, 1121
AlwI GGATC 3 cut(s) 59, 695, 1030
AoxI GGCC 4 cut(s) 108, 766, 825, 849
ApeKI GCWGC 4 cut(s) 74, 119, 658, 760
ApoI RAATTY 3 cut(s) 8, 500, 593
AspLEI GCGC 2 cut(s) 74, 1027
AspS9I GGNCC 3 cut(s) 328, 767, 1139
AsuHPI GGTGA 4 cut(s) 586, 736, 1045, 1208
AvaII GGWCC 2 cut(s) 328, 1139
AxyI CCTNAGG 1 cut(s) 879
BalI TGGCCA 1 cut(s) 827
BanI GGYRCC 1 cut(s) 1024
BanII GRGCYC 2 cut(s) 642, 743
Bbv12I GWGCWC 3 cut(s) 530, 642, 743
BbvI GCAGC 4 cut(s) 86, 131, 670, 747
BccI CCATC 2 cut(s) 468, 979
BceAI ACGGC 2 cut(s) 63, 781
BcgI CGANNNNNNTGC 2 cut(s) 647, 681
BciT130I CCWGG 2 cut(s) 771, 829
BcoDI GTCTC 3 cut(s) 45, 625, 1121
BfaI CTAG 3 cut(s) 69, 440, 906
BfoI RGCGCY 1 cut(s) 1028
BfuAI ACCTGC 1 cut(s) 190
BglI GCCNNNNNGGC 1 cut(s) 116
BisI GCNGC 5 cut(s) 75, 120, 131, 659, 761
BlsI GCNGC 5 cut(s) 76, 121, 132, 660, 762
Bme1390I CCNGG 2 cut(s) 771, 829
Bme18I GGWCC 2 cut(s) 328, 1139
BmeRI GACNNNNNGTC 1 cut(s) 566
BmgT120I GGNCC 3 cut(s) 328, 767, 1139
BmiI GGNNCC 2 cut(s) 129, 1026
BmrFI CCNGG 2 cut(s) 771, 829
BmsI GCATC 1 cut(s) 433
BoxI GACNNNNGTC 1 cut(s) 930
Bpu10I CCTNAGC 1 cut(s) 846
BpuEI CTTGAG 4 cut(s) 618, 757, 991, 1165
Bsa29I ATCGAT 1 cut(s) 453
BsaAI YACGTR 3 cut(s) 673, 937, 1154
BsaHI GRCGYC 1 cut(s) 1025
BsaI GGTCTC 1 cut(s) 45
BsaJI CCNNGG 2 cut(s) 769, 828
Bse118I RCCGGY 1 cut(s) 106
Bse1I ACTGG 5 cut(s) 175, 216, 382, 387, 1111
Bse21I CCTNAGG 1 cut(s) 879
BseBI CCWGG 2 cut(s) 771, 829
BseCI ATCGAT 1 cut(s) 453
BseDI CCNNGG 2 cut(s) 769, 828
BseGI GGATG 4 cut(s) 252, 479, 798, 928
BseMII CTCAG 1 cut(s) 860
BseNI ACTGG 5 cut(s) 175, 216, 382, 387, 1111
BseRI GAGGAG 2 cut(s) 399, 995
BseXI GCAGC 4 cut(s) 86, 131, 670, 747
BsgI GTGCAG 2 cut(s) 398, 746
Bsh1236I CGCG 2 cut(s) 572, 894
BshFI GGCC 4 cut(s) 110, 768, 827, 851
BshNI GGYRCC 1 cut(s) 1024
BshVI ATCGAT 1 cut(s) 453
BsiHKAI GWGCWC 3 cut(s) 530, 642, 743
BsiSI CCGG 1 cut(s) 107
BsmAI GTCTC 3 cut(s) 45, 625, 1121
BsnI GGCC 4 cut(s) 110, 768, 827, 851
Bso31I GGTCTC 1 cut(s) 45
Bsp1286I GDGCHC 3 cut(s) 530, 642, 743
Bsp1407I TGTACA 2 cut(s) 252, 1017
Bsp143I GATC 7 cut(s) 64, 349, 436, 454, 700, 840, 1035
Bsp68I TCGCGA 1 cut(s) 894
BspACI CCGC 2 cut(s) 131, 572
BspANI GGCC 4 cut(s) 110, 768, 827, 851
BspCNI CTCAG 1 cut(s) 859
BspDI ATCGAT 1 cut(s) 453
BspFNI CGCG 2 cut(s) 572, 894
BspLI GGNNCC 2 cut(s) 129, 1026
BspMI ACCTGC 1 cut(s) 190
BspPI GGATC 3 cut(s) 59, 695, 1030
BspT107I GGYRCC 1 cut(s) 1024
BspTNI GGTCTC 1 cut(s) 45
BsrFI RCCGGY 1 cut(s) 106
BsrGI TGTACA 2 cut(s) 252, 1017
BsrI ACTGG 5 cut(s) 175, 216, 382, 387, 1111
BssAI RCCGGY 1 cut(s) 106
BssECI CCNNGG 2 cut(s) 769, 828
BssMI GATC 7 cut(s) 64, 349, 436, 454, 700, 840, 1035
BssNAI GTATAC 1 cut(s) 1072
BssNI GRCGYC 1 cut(s) 1025
Bst1107I GTATAC 1 cut(s) 1072
Bst2UI CCWGG 2 cut(s) 771, 829
Bst4CI ACNGT 3 cut(s) 115, 137, 364
Bst6I CTCTTC 3 cut(s) 501, 810, 1180
BstACI GRCGYC 1 cut(s) 1025
BstAUI TGTACA 2 cut(s) 252, 1017
BstBAI YACGTR 3 cut(s) 673, 937, 1154
BstC8I GCNNGC 3 cut(s) 108, 694, 1046
BstDEI CTNAG 2 cut(s) 846, 879
BstEII GGTNACC 1 cut(s) 724
BstF5I GGATG 4 cut(s) 252, 479, 798, 928
BstFNI CGCG 2 cut(s) 572, 894
BstH2I RGCGCY 1 cut(s) 1028
BstHHI GCGC 2 cut(s) 74, 1027
BstKTI GATC 7 cut(s) 67, 352, 439, 457, 703, 843, 1038
BstMAI GTCTC 3 cut(s) 45, 625, 1121
BstMBI GATC 7 cut(s) 64, 349, 436, 454, 700, 840, 1035
BstMWI GCNNNNNNNGC 2 cut(s) 88, 116
BstNI CCWGG 2 cut(s) 771, 829
BstPAI GACNNNNGTC 1 cut(s) 930
BstPI GGTNACC 1 cut(s) 724
BstSCI CCNGG 2 cut(s) 769, 827
BstSNI TACGTA 1 cut(s) 1154
BstUI CGCG 2 cut(s) 572, 894
BstV1I GCAGC 4 cut(s) 86, 131, 670, 747
BstXI CCANNNNNNTGG 3 cut(s) 190, 317, 835
BstZ17I GTATAC 1 cut(s) 1072
Bsu15I ATCGAT 1 cut(s) 453
Bsu36I CCTNAGG 1 cut(s) 879
BsuRI GGCC 4 cut(s) 110, 768, 827, 851
BsuTUI ATCGAT 1 cut(s) 453
BtgZI GCGATG 1 cut(s) 875
BtsCI GGATG 4 cut(s) 252, 479, 798, 928
BtsIMutI CAGTG 3 cut(s) 111, 360, 375
BtuMI TCGCGA 1 cut(s) 894
BveI ACCTGC 1 cut(s) 190
Cac8I GCNNGC 3 cut(s) 108, 694, 1046
CfoI GCGC 2 cut(s) 74, 1027
Cfr10I RCCGGY 1 cut(s) 106
Cfr13I GGNCC 3 cut(s) 328, 767, 1139
ClaI ATCGAT 1 cut(s) 453
CseI GACGC 2 cut(s) 650, 935
Csp6I GTAC 6 cut(s) 253, 335, 670, 1018, 1151, 1161
CviAII CATG 5 cut(s) 353, 552, 557, 853, 1021
CviQI GTAC 6 cut(s) 253, 335, 670, 1018, 1151, 1161
DdeI CTNAG 2 cut(s) 846, 879
DinI GGCGCC 1 cut(s) 1026
DpnI GATC 7 cut(s) 66, 351, 438, 456, 702, 842, 1037
DpnII GATC 7 cut(s) 64, 349, 436, 454, 700, 840, 1035
DriI GACNNNNNGTC 1 cut(s) 566
EaeI YGGCCR 2 cut(s) 108, 825
Eam1104I CTCTTC 3 cut(s) 501, 810, 1180
Eam1105I GACNNNNNGTC 1 cut(s) 566
EarI CTCTTC 3 cut(s) 501, 810, 1180
Ecl136II GAGCTC 2 cut(s) 640, 741
Eco105I TACGTA 1 cut(s) 1154
Eco24I GRGCYC 2 cut(s) 642, 743
Eco31I GGTCTC 1 cut(s) 45
Eco47I GGWCC 2 cut(s) 328, 1139
Eco53kI GAGCTC 2 cut(s) 640, 741
Eco81I CCTNAGG 1 cut(s) 879
Eco91I GGTNACC 1 cut(s) 724
EcoICRI GAGCTC 2 cut(s) 640, 741
EcoO65I GGTNACC 1 cut(s) 724
EcoRI GAATTC 1 cut(s) 593
EcoRII CCWGG 2 cut(s) 769, 827
EcoT22I ATGCAT 1 cut(s) 584
EcoT38I GRGCYC 2 cut(s) 642, 743
EgeI GGCGCC 1 cut(s) 1026
EheI GGCGCC 1 cut(s) 1026
FaeI CATG 5 cut(s) 356, 555, 560, 856, 1024
FatI CATG 5 cut(s) 352, 551, 556, 852, 1020
FauNDI CATATG 1 cut(s) 1012
FblI GTMKAC 3 cut(s) 179, 298, 1071
Fnu4HI GCNGC 5 cut(s) 75, 120, 131, 659, 761
FokI GGATG 4 cut(s) 259, 486, 805, 935
FriOI GRGCYC 2 cut(s) 642, 743
Fsp4HI GCNGC 5 cut(s) 75, 120, 131, 659, 761
FspBI CTAG 3 cut(s) 69, 440, 906
GlaI GCGC 2 cut(s) 73, 1026
GluI GCNGC 5 cut(s) 75, 120, 131, 659, 761
HaeII RGCGCY 1 cut(s) 1028
HaeIII GGCC 4 cut(s) 110, 768, 827, 851
HapII CCGG 1 cut(s) 107
HgaI GACGC 2 cut(s) 650, 935
HhaI GCGC 2 cut(s) 74, 1027
Hin1I GRCGYC 1 cut(s) 1025
Hin1II CATG 5 cut(s) 356, 555, 560, 856, 1024
Hin6I GCGC 2 cut(s) 72, 1025
HinP1I GCGC 2 cut(s) 72, 1025
HincII GTYRAC 2 cut(s) 157, 367
HindII GTYRAC 2 cut(s) 157, 367
HinfI GANTC 2 cut(s) 149, 602
HpaI GTTAAC 1 cut(s) 367
HpaII CCGG 1 cut(s) 107
HphI GGTGA 4 cut(s) 586, 736, 1045, 1208
Hpy166II GTNNAC 6 cut(s) 157, 180, 299, 367, 1018, 1072
Hpy188I TCNGA 3 cut(s) 27, 246, 436
Hpy188III TCNNGA 7 cut(s) 146, 440, 635, 736, 893, 970, 1081
Hpy8I GTNNAC 6 cut(s) 157, 180, 299, 367, 1018, 1072
HpyAV CCTTC 1 cut(s) 441
HpyCH4III ACNGT 3 cut(s) 115, 137, 364
HpyCH4IV ACGT 6 cut(s) 159, 647, 672, 936, 1153, 1163
HpyCH4V TGCA 5 cut(s) 415, 582, 763, 856, 947
HpyF10VI GCNNNNNNNGC 2 cut(s) 88, 116
HpyF3I CTNAG 2 cut(s) 846, 879
HpySE526I ACGT 6 cut(s) 159, 647, 672, 936, 1153, 1163
Hsp92I GRCGYC 1 cut(s) 1025
Hsp92II CATG 5 cut(s) 356, 555, 560, 856, 1024
HspAI GCGC 2 cut(s) 72, 1025
KasI GGCGCC 1 cut(s) 1024
KroI GCCGGC 1 cut(s) 106
KroNI GCCGGC 1 cut(s) 108
KspAI GTTAAC 1 cut(s) 367
Kzo9I GATC 7 cut(s) 64, 349, 436, 454, 700, 840, 1035
LmnI GCTCC 2 cut(s) 127, 525
Lsp1109I GCAGC 4 cut(s) 86, 131, 670, 747
LweI GCATC 1 cut(s) 433
MaeI CTAG 3 cut(s) 69, 440, 906
MaeII ACGT 6 cut(s) 159, 647, 672, 936, 1153, 1163
MaeIII GTNAC 4 cut(s) 85, 724, 932, 956
MalI GATC 7 cut(s) 66, 351, 438, 456, 702, 842, 1037
MboI GATC 7 cut(s) 64, 349, 436, 454, 700, 840, 1035
MboII GAAGA 6 cut(s) 40, 518, 523, 827, 878, 1167
MfeI CAATTG 1 cut(s) 395
MhlI GDGCHC 3 cut(s) 530, 642, 743
MlsI TGGCCA 1 cut(s) 827
MluCI AATT 8 cut(s) 8, 60, 221, 263, 395, 500, 593, 1111
MluNI TGGCCA 1 cut(s) 827
Mly113I GGCGCC 1 cut(s) 1025
MmeI TCCRAC 2 cut(s) 68, 171
Mox20I TGGCCA 1 cut(s) 827
Mph1103I ATGCAT 1 cut(s) 584
MroNI GCCGGC 1 cut(s) 106
MscI TGGCCA 1 cut(s) 827
MseI TTAA 4 cut(s) 366, 1166, 1203, 1222
Msp20I TGGCCA 1 cut(s) 827
MspA1I CMGCKG 1 cut(s) 122
MspI CCGG 1 cut(s) 107
MspR9I CCNGG 2 cut(s) 771, 829
MunI CAATTG 1 cut(s) 395
MvaI CCWGG 2 cut(s) 771, 829
MvnI CGCG 2 cut(s) 572, 894
MwoI GCNNNNNNNGC 2 cut(s) 88, 116
NaeI GCCGGC 1 cut(s) 108
NarI GGCGCC 1 cut(s) 1025
NdeI CATATG 1 cut(s) 1012
NdeII GATC 7 cut(s) 64, 349, 436, 454, 700, 840, 1035
NgoMIV GCCGGC 1 cut(s) 106
NlaIII CATG 5 cut(s) 356, 555, 560, 856, 1024
NlaIV GGNNCC 2 cut(s) 129, 1026
NmeAIII GCCGAG 1 cut(s) 81
NmuCI GTSAC 3 cut(s) 724, 932, 956
NruI TCGCGA 1 cut(s) 894
NsiI ATGCAT 1 cut(s) 584
PaqCI CACCTGC 1 cut(s) 190
PcsI WCGNNNNNNNCGW 2 cut(s) 53, 933
PdiI GCCGGC 1 cut(s) 108
PfeI GAWTC 2 cut(s) 149, 602
PkrI GCNGC 5 cut(s) 76, 121, 132, 660, 762
PluTI GGCGCC 1 cut(s) 1028
Ppu21I YACGTR 3 cut(s) 673, 937, 1154
PshAI GACNNNNGTC 1 cut(s) 930
PsiI TTATAA 1 cut(s) 1103
Psp124BI GAGCTC 2 cut(s) 642, 743
Psp1406I AACGTT 1 cut(s) 159
Psp6I CCWGG 2 cut(s) 769, 827
PspEI GGTNACC 1 cut(s) 724
PspGI CCWGG 2 cut(s) 769, 827
PspN4I GGNNCC 2 cut(s) 129, 1026
PspPI GGNCC 3 cut(s) 328, 767, 1139
PvuII CAGCTG 1 cut(s) 122
RruI TCGCGA 1 cut(s) 894
RsaI GTAC 6 cut(s) 254, 336, 671, 1019, 1152, 1162
RsaNI GTAC 6 cut(s) 253, 335, 670, 1018, 1151, 1161
SacI GAGCTC 2 cut(s) 642, 743
SaqAI TTAA 4 cut(s) 366, 1166, 1203, 1222
SatI GCNGC 5 cut(s) 75, 120, 131, 659, 761
Sau3AI GATC 7 cut(s) 64, 349, 436, 454, 700, 840, 1035
Sau96I GGNCC 3 cut(s) 328, 767, 1139
ScrFI CCNGG 2 cut(s) 771, 829
SduI GDGCHC 3 cut(s) 530, 642, 743
SfaNI GCATC 1 cut(s) 433
SfoI GGCGCC 1 cut(s) 1026
SinI GGWCC 2 cut(s) 328, 1139
SmlI CTYRAG 4 cut(s) 633, 736, 970, 1180
SmoI CTYRAG 4 cut(s) 633, 736, 970, 1180
SnaBI TACGTA 1 cut(s) 1154
Sse9I AATT 8 cut(s) 8, 60, 221, 263, 395, 500, 593, 1111
SsiI CCGC 2 cut(s) 131, 572
SspDI GGCGCC 1 cut(s) 1024
SspMI CTAG 3 cut(s) 69, 440, 906
SstI GAGCTC 2 cut(s) 642, 743
StyD4I CCNGG 2 cut(s) 769, 827
TaaI ACNGT 3 cut(s) 115, 137, 364
TaiI ACGT 6 cut(s) 162, 650, 675, 939, 1156, 1166
TaqI TCGA 5 cut(s) 145, 167, 453, 642, 743
TaqII GACCGA 2 cut(s) 142, 743
TasI AATT 8 cut(s) 8, 60, 221, 263, 395, 500, 593, 1111
TatI WGTACW 3 cut(s) 252, 334, 1017
TauI GCSGC 1 cut(s) 133
TfiI GAWTC 2 cut(s) 149, 602
Tru1I TTAA 4 cut(s) 366, 1166, 1203, 1222
Tru9I TTAA 4 cut(s) 366, 1166, 1203, 1222
TscAI CASTG 3 cut(s) 118, 367, 382
TseFI GTSAC 3 cut(s) 724, 932, 956
TseI GCWGC 4 cut(s) 74, 119, 658, 760
Tsp45I GTSAC 3 cut(s) 724, 932, 956
TspDTI ATGAA 5 cut(s) 17, 469, 668, 878, 1211
TspRI CASTG 3 cut(s) 118, 367, 382
VpaK11BI GGWCC 2 cut(s) 328, 1139
XapI RAATTY 3 cut(s) 8, 500, 593
XbaI TCTAGA 1 cut(s) 439
XmiI GTMKAC 3 cut(s) 179, 298, 1071
XspI CTAG 3 cut(s) 69, 440, 906
Zsp2I ATGCAT 1 cut(s) 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.