MD15G1052200.v1.1
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0014670
Physical Location & Seq
Reverse (-)
3584810 .. 3586448
1639 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1052200.v1.1.491

Sequence Viewer

Length: 1080 bp
ATGATGGAAAGAACACAAGATTATCTTGGCCACTGCCTACAAGTAGCCCAAGATAATGGATTCTTGAACCTTATTCACAACTCCAAAGACATTGGCCCACAACCATGCCCTCCCTCCTCCAATGTTGCCACTTCAAGTCCAAATGCCACCCCACAATTTCCAGCAACTCAACCACACCATTCTTCTGATCTAGCAGCTGTTACTGAGCAAGCCCAGCTAAATGGATGGTACATCCACCCAGGAGAGATTGAGATTGACGAAAAAATAGGGCAGGGAAGTACGGCTCACATATACAAAGGAACATGGCATGGACTTGAAGTGGCAGTGAAATGCATGTACCCTGATTTCTTTGAAACTAACGACAATGGTGTCGCATTTTTCGCTCAAGAGCTTGACACCTTACGTAGGCAGCGACATCCATACATACTTCAGCTAATGGGTGCATGCCTCGATCCTCCTAACTATGCTTGGGTGGTGACGGAGTTCATGAGCACAACACTGAAGGAGTGGCTTCACGGCCCCGGAAATAGGCGAAAAGAGAGGATGATACCACTTCCTCCGCTCAAAGAGAGGGTCGCTAGGGCATTGGAGATTGCTCTAGCCATGCAGTATCTTCATGGACAAAGGCCTATGGTGATTCATCGTGATCTCAAGCCTAGCAACATATTTTTGGACGATGCTATGCATGTACGAGTTGCAGATTTTGGTCATGCTCGGTTCTTGAGTGATGAAGAGAAGGCACTCACCGGCGAAACGGGAACTTATGTGTACATGGCACCTGAGGTCATCCAATGCGAGCCTTATAACGAAAAATGCGACGTATACAGTTTTGGGATTTTATTGAATGAGCTTATAACTGGAGATTATCCTTATATTGAGAAGGATTATGGTCCCATTAAGATTGCAATGGAAGTTGTGGAGGAGAACCTAAGGCCAAAACTGGCTGATGATCGTGATGGGCAACTTGGGGAACTAATTGACCTAATTTGCCTTTCATGGAATGCAGATGCGTCAGTGAGACCATCCTTTGCCGCCATTACAAGCAGTTTGAAGGCTCTTCAAAACAGATTATTTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

360

Amino Acids

40.54

Weight (kDa)

5.23

Isoelectric Point (pI)

45.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 83 - 350 1.6e-43 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 84 - 347 1.2e-44 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014670)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g31250
malus_domestica MD08G1069800.v1.1 MD15G1052200.v1.1
prunus_persica Prupe.1G406200_v2.0.a1 Prupe.1G406200_v2.0.a1
pyrus_communis pycom15g05000
rosa_chinensis RchiOBHm_Chr6g0311551
rosa_laevigata RLG00000010394
rosa_multiflora Rmu_co8269907.1_g000001 Rmu_sc0007556.1_g000001
rosa_roxburghii Rroxscaffold_7G00157500
rosa_rugosa Rorug06G0389700
rosa_samantha Rh6AG504600 Rh6BG515300 Rh6CG521200 Rh6DG507100
rosa_wichuraiana Rw6G043940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 804, 854
AasI GACNNNNNNGTC 1 cut(s) 368
AccB1I GGYRCC 1 cut(s) 775
AccBSI CCGCTC 1 cut(s) 562
AccI GTMKAC 1 cut(s) 822
AciI CCGC 2 cut(s) 560, 1032
AclWI GGATC 1 cut(s) 446
AcoI YGGCCR 1 cut(s) 28
AcuI CTGAAG 2 cut(s) 413, 521
AfaI GTAC 5 cut(s) 230, 280, 338, 690, 770
AfiI CCNNNNNNNGG 2 cut(s) 405, 528
AgsI TTSAA 7 cut(s) 67, 135, 317, 353, 844, 1051, 1061
AjnI CCWGG 1 cut(s) 238
AluBI AGCT 5 cut(s) 197, 217, 391, 433, 850
AluI AGCT 5 cut(s) 197, 217, 391, 433, 850
Alw21I GWGCWC 1 cut(s) 494
Alw26I GTCTC 1 cut(s) 1012
AlwI GGATC 1 cut(s) 446
AoxI GGCC 5 cut(s) 28, 94, 517, 626, 932
ApeKI GCWGC 2 cut(s) 194, 409
ArsI GACNNNNNNTTYG 2 cut(s) 558, 590
AspS9I GGNCC 3 cut(s) 95, 518, 890
AsuC2I CCSGG 1 cut(s) 522
AsuHPI GGTGA 3 cut(s) 487, 646, 736
AvaII GGWCC 1 cut(s) 890
AxyI CCTNAGG 2 cut(s) 780, 929
BalI TGGCCA 1 cut(s) 30
BanI GGYRCC 1 cut(s) 775
Bbv12I GWGCWC 1 cut(s) 494
BbvI GCAGC 2 cut(s) 206, 421
BccI CCATC 3 cut(s) 219, 950, 1030
BceAI ACGGC 2 cut(s) 297, 532
BciT130I CCWGG 1 cut(s) 240
BcnI CCSGG 1 cut(s) 522
BcoDI GTCTC 1 cut(s) 1012
BfaI CTAG 4 cut(s) 191, 579, 599, 657
BisI GCNGC 3 cut(s) 195, 410, 1032
BlsI GCNGC 3 cut(s) 196, 411, 1033
Bme1390I CCNGG 2 cut(s) 240, 522
Bme18I GGWCC 1 cut(s) 890
BmgT120I GGNCC 3 cut(s) 95, 518, 890
BmiI GGNNCC 3 cut(s) 520, 777, 892
BmrFI CCNGG 2 cut(s) 240, 522
BmsI GCATC 2 cut(s) 667, 997
BpmI CTGGAG 1 cut(s) 879
BpuEI CTTGAG 3 cut(s) 369, 635, 742
BpuMI CCSGG 1 cut(s) 522
BsaAI YACGTR 1 cut(s) 404
BsaI GGTCTC 1 cut(s) 1012
BsaJI CCNNGG 2 cut(s) 238, 520
Bsc4I CCNNNNNNNGG 2 cut(s) 405, 528
Bse118I RCCGGY 1 cut(s) 746
Bse1I ACTGG 2 cut(s) 862, 945
Bse21I CCTNAGG 2 cut(s) 780, 929
Bse3DI GCAATG 1 cut(s) 912
BseBI CCWGG 1 cut(s) 240
BseDI CCNNGG 2 cut(s) 238, 520
BseGI GGATG 6 cut(s) 230, 231, 415, 549, 786, 1022
BseLI CCNNNNNNNGG 2 cut(s) 405, 528
BseMI GCAATG 1 cut(s) 912
BseMII CTCAG 2 cut(s) 195, 771
BseNI ACTGG 2 cut(s) 862, 945
BseRI GAGGAG 2 cut(s) 106, 935
BseXI GCAGC 2 cut(s) 206, 421
BseYI CCCAGC 1 cut(s) 213
BshFI GGCC 5 cut(s) 30, 96, 519, 628, 934
BshNI GGYRCC 1 cut(s) 775
BsiHKAI GWGCWC 1 cut(s) 494
BsiSI CCGG 2 cut(s) 522, 747
BslFI GGGAC 1 cut(s) 876
BslI CCNNNNNNNGG 2 cut(s) 405, 528
BsmAI GTCTC 1 cut(s) 1012
BsmFI GGGAC 1 cut(s) 876
BsmI GAATGC 1 cut(s) 1006
BsnI GGCC 5 cut(s) 30, 96, 519, 628, 934
Bso31I GGTCTC 1 cut(s) 1012
Bsp1286I GDGCHC 1 cut(s) 494
Bsp1407I TGTACA 1 cut(s) 768
Bsp143I GATC 4 cut(s) 187, 451, 646, 949
BspACI CCGC 2 cut(s) 560, 1032
BspANI GGCC 5 cut(s) 30, 96, 519, 628, 934
BspCNI CTCAG 2 cut(s) 196, 772
BspHI TCATGA 1 cut(s) 486
BspLI GGNNCC 3 cut(s) 520, 777, 892
BspPI GGATC 1 cut(s) 446
BspQI GCTCTTC 1 cut(s) 1062
BspT107I GGYRCC 1 cut(s) 775
BspTNI GGTCTC 1 cut(s) 1012
BsrBI CCGCTC 1 cut(s) 562
BsrDI GCAATG 1 cut(s) 912
BsrFI RCCGGY 1 cut(s) 746
BsrGI TGTACA 1 cut(s) 768
BsrI ACTGG 2 cut(s) 862, 945
BssAI RCCGGY 1 cut(s) 746
BssECI CCNNGG 2 cut(s) 238, 520
BssMI GATC 4 cut(s) 187, 451, 646, 949
BssNAI GTATAC 1 cut(s) 823
Bst1107I GTATAC 1 cut(s) 823
Bst2UI CCWGG 1 cut(s) 240
Bst4CI ACNGT 1 cut(s) 827
Bst6I CTCTTC 2 cut(s) 726, 1062
BstAUI TGTACA 1 cut(s) 768
BstBAI YACGTR 1 cut(s) 404
BstC8I GCNNGC 3 cut(s) 210, 445, 797
BstDEI CTNAG 3 cut(s) 204, 780, 929
BstENI CCTNNNNNAGG 1 cut(s) 403
BstF5I GGATG 6 cut(s) 230, 231, 415, 549, 786, 1022
BstKTI GATC 4 cut(s) 190, 454, 649, 952
BstMAI GTCTC 1 cut(s) 1012
BstMBI GATC 4 cut(s) 187, 451, 646, 949
BstMWI GCNNNNNNNGC 2 cut(s) 214, 380
BstNI CCWGG 1 cut(s) 240
BstNSI RCATGY 3 cut(s) 337, 447, 689
BstSCI CCNGG 2 cut(s) 238, 520
BstSNI TACGTA 1 cut(s) 404
BstV1I GCAGC 2 cut(s) 206, 421
BstXI CCANNNNNNTGG 2 cut(s) 56, 221
BstZ17I GTATAC 1 cut(s) 823
Bsu36I CCTNAGG 2 cut(s) 780, 929
BsuRI GGCC 5 cut(s) 30, 96, 519, 628, 934
BtsCI GGATG 6 cut(s) 230, 231, 415, 549, 786, 1022
BtsI GCAGTG 2 cut(s) 31, 330
BtsIMutI CAGTG 4 cut(s) 31, 330, 497, 1020
Cac8I GCNNGC 3 cut(s) 210, 445, 797
CciI TCATGA 1 cut(s) 486
Cfr10I RCCGGY 1 cut(s) 746
Cfr13I GGNCC 3 cut(s) 95, 518, 890
CseI GACGC 1 cut(s) 999
Csp6I GTAC 5 cut(s) 229, 279, 337, 689, 769
CviQI GTAC 5 cut(s) 229, 279, 337, 689, 769
DdeI CTNAG 3 cut(s) 204, 780, 929
DpnI GATC 4 cut(s) 189, 453, 648, 951
DpnII GATC 4 cut(s) 187, 451, 646, 949
DrdI GACNNNNNNGTC 1 cut(s) 368
DseDI GACNNNNNNGTC 1 cut(s) 368
EaeI YGGCCR 1 cut(s) 28
Eam1104I CTCTTC 2 cut(s) 726, 1062
EarI CTCTTC 2 cut(s) 726, 1062
Eco105I TACGTA 1 cut(s) 404
Eco147I AGGCCT 1 cut(s) 628
Eco31I GGTCTC 1 cut(s) 1012
Eco47I GGWCC 1 cut(s) 890
Eco57I CTGAAG 2 cut(s) 413, 521
Eco81I CCTNAGG 2 cut(s) 780, 929
EcoNI CCTNNNNNAGG 1 cut(s) 403
EcoRII CCWGG 1 cut(s) 238
EcoT22I ATGCAT 2 cut(s) 335, 687
FalI AAGNNNNNCTT 2 cut(s) 9, 41
FaqI GGGAC 1 cut(s) 876
FblI GTMKAC 1 cut(s) 822
Fnu4HI GCNGC 3 cut(s) 195, 410, 1032
FokI GGATG 6 cut(s) 218, 237, 402, 556, 773, 1009
Fsp4HI GCNGC 3 cut(s) 195, 410, 1032
FspBI CTAG 4 cut(s) 191, 579, 599, 657
GluI GCNGC 3 cut(s) 195, 410, 1032
GsaI CCCAGC 1 cut(s) 217
GsuI CTGGAG 1 cut(s) 879
HaeIII GGCC 5 cut(s) 30, 96, 519, 628, 934
HapII CCGG 2 cut(s) 522, 747
HgaI GACGC 1 cut(s) 999
HinfI GANTC 2 cut(s) 60, 637
HpaII CCGG 2 cut(s) 522, 747
HphI GGTGA 3 cut(s) 487, 646, 736
Hpy166II GTNNAC 2 cut(s) 769, 823
Hpy188I TCNGA 1 cut(s) 187
Hpy188III TCNNGA 6 cut(s) 64, 386, 487, 644, 721, 953
Hpy8I GTNNAC 2 cut(s) 769, 823
Hpy99I CGWCG 1 cut(s) 821
HpyAV CCTTC 4 cut(s) 496, 730, 874, 1045
HpyCH4III ACNGT 1 cut(s) 827
HpyCH4IV ACGT 2 cut(s) 403, 819
HpyCH4V TGCA 7 cut(s) 333, 443, 607, 685, 698, 905, 1004
HpyF10VI GCNNNNNNNGC 2 cut(s) 214, 380
HpyF3I CTNAG 3 cut(s) 204, 780, 929
HpySE526I ACGT 2 cut(s) 403, 819
Kzo9I GATC 4 cut(s) 187, 451, 646, 949
LguI GCTCTTC 1 cut(s) 1062
Lsp1109I GCAGC 2 cut(s) 206, 421
LweI GCATC 2 cut(s) 667, 997
MaeI CTAG 4 cut(s) 191, 579, 599, 657
MaeII ACGT 2 cut(s) 403, 819
MaeIII GTNAC 2 cut(s) 199, 475
MalI GATC 4 cut(s) 189, 453, 648, 951
MbiI CCGCTC 1 cut(s) 562
MboI GATC 4 cut(s) 187, 451, 646, 949
MboII GAAGA 4 cut(s) 174, 605, 743, 1049
MhlI GDGCHC 1 cut(s) 494
MlsI TGGCCA 1 cut(s) 30
MluCI AATT 3 cut(s) 155, 975, 984
MluNI TGGCCA 1 cut(s) 30
Mox20I TGGCCA 1 cut(s) 30
Mph1103I ATGCAT 2 cut(s) 335, 687
MscI TGGCCA 1 cut(s) 30
MseI TTAA 1 cut(s) 897
MslI CAYNNNNRTG 1 cut(s) 103
Msp20I TGGCCA 1 cut(s) 30
MspA1I CMGCKG 1 cut(s) 197
MspI CCGG 2 cut(s) 522, 747
MspR9I CCNGG 2 cut(s) 240, 522
Mva1269I GAATGC 1 cut(s) 1006
MvaI CCWGG 1 cut(s) 240
MwoI GCNNNNNNNGC 2 cut(s) 214, 380
NciI CCSGG 1 cut(s) 522
NdeII GATC 4 cut(s) 187, 451, 646, 949
NlaIV GGNNCC 3 cut(s) 520, 777, 892
NmuCI GTSAC 1 cut(s) 475
NsiI ATGCAT 2 cut(s) 335, 687
NspI RCATGY 3 cut(s) 337, 447, 689
PaeI GCATGC 1 cut(s) 447
PagI TCATGA 1 cut(s) 486
PceI AGGCCT 1 cut(s) 628
PciSI GCTCTTC 1 cut(s) 1062
PcsI WCGNNNNNNNCGW 2 cut(s) 409, 813
PctI GAATGC 1 cut(s) 1006
PfeI GAWTC 2 cut(s) 60, 637
PkrI GCNGC 3 cut(s) 196, 411, 1033
Ppu21I YACGTR 1 cut(s) 404
PsiI TTATAA 2 cut(s) 804, 854
Psp6I CCWGG 1 cut(s) 238
PspFI CCCAGC 1 cut(s) 213
PspGI CCWGG 1 cut(s) 238
PspN4I GGNNCC 3 cut(s) 520, 777, 892
PspPI GGNCC 3 cut(s) 95, 518, 890
PvuII CAGCTG 1 cut(s) 197
RsaI GTAC 5 cut(s) 230, 280, 338, 690, 770
RsaNI GTAC 5 cut(s) 229, 279, 337, 689, 769
RseI CAYNNNNRTG 1 cut(s) 103
SapI GCTCTTC 1 cut(s) 1062
SaqAI TTAA 1 cut(s) 897
SatI GCNGC 3 cut(s) 195, 410, 1032
Sau3AI GATC 4 cut(s) 187, 451, 646, 949
Sau96I GGNCC 3 cut(s) 95, 518, 890
ScrFI CCNGG 2 cut(s) 240, 522
SduI GDGCHC 1 cut(s) 494
SfaNI GCATC 2 cut(s) 667, 997
SgrAI CRCCGGYG 1 cut(s) 746
SinI GGWCC 1 cut(s) 890
SmiMI CAYNNNNRTG 1 cut(s) 103
SmlI CTYRAG 3 cut(s) 384, 650, 721
SmoI CTYRAG 3 cut(s) 384, 650, 721
SnaBI TACGTA 1 cut(s) 404
SphI GCATGC 1 cut(s) 447
Sse9I AATT 3 cut(s) 155, 975, 984
SseBI AGGCCT 1 cut(s) 628
SsiI CCGC 2 cut(s) 560, 1032
SspMI CTAG 4 cut(s) 191, 579, 599, 657
StuI AGGCCT 1 cut(s) 628
StyD4I CCNGG 2 cut(s) 238, 520
TaaI ACNGT 1 cut(s) 827
TaiI ACGT 2 cut(s) 406, 822
TaqI TCGA 1 cut(s) 450
TasI AATT 3 cut(s) 155, 975, 984
TatI WGTACW 1 cut(s) 768
TauI GCSGC 1 cut(s) 1034
TfiI GAWTC 2 cut(s) 60, 637
Tru1I TTAA 1 cut(s) 897
Tru9I TTAA 1 cut(s) 897
TscAI CASTG 4 cut(s) 38, 330, 504, 1020
TseFI GTSAC 1 cut(s) 475
TseI GCWGC 2 cut(s) 194, 409
Tsp45I GTSAC 1 cut(s) 475
TspDTI ATGAA 5 cut(s) 475, 605, 629, 744, 984
TspGWI ACGGA 1 cut(s) 494
TspRI CASTG 4 cut(s) 38, 330, 504, 1020
VpaK11BI GGWCC 1 cut(s) 890
XagI CCTNNNNNAGG 1 cut(s) 403
XceI RCATGY 3 cut(s) 337, 447, 689
XmiI GTMKAC 1 cut(s) 822
XspI CTAG 4 cut(s) 191, 579, 599, 657
Zsp2I ATGCAT 2 cut(s) 335, 687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.