Prupe.1G406200_v2.0.a1
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0014670
Physical Location & Seq
Reverse (-)
35683330 .. 35686672
3343 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G406200.1

Sequence Viewer

Length: 1320 bp
ATGAACCATCAGAAGAAAAAGGGCGGCGCGTTGTTTGGGTGGTCTCCTCAGTCGAGCAGTGCAAAGCAAGCGACGGCGACAACAACAACGGGATGCTGTGGAGGGATTCATGGCTGCTCAAGCTTGGAGGCAATTAACGTTGTTCCTCCTACGAACCAAAATTCAACCTCTAAATTGGAGCTGCAGGTAGCTGAACTTGAAAAGAAAGTCCTAAAGGAGAAAGAACTTCGGGCGGTGTACAAGAAGAGGATGGAAAGAACACAAGATTATCTGAGGAACTGCCTACAAGTGGCCCAAGATAATGGATTCTTGGACCTCATTCACAACTACAACAATAATATGGGTCCAGAACCAGGCCTTCTCTCCCCCAATATTCTCACTCCCAATACCAGCCCTCGATTGCCAACAACTCCTCTATACCAACCGTCGGATCTAGCTGTTCTTATTGATCAAGCCCACCTAAACGGATGGTACATCCATCCGGAAGAGATTGAATTCGAAGAGAAAATAGGGCAGGGAAGCACGGCTCACATATACAAAGGAACATGGCATGGGCTTGATGTGGCAATCAAATGCATATTCCCAGATTTCTTTGAATCGAATGAGAACGGTATCTCGTTTTTCGCACAAGAGCTCGACACATTATGCCGGCAGCGCCATAGATATGTACTTCAACTAATGGGCGCATGCCTCGATCCTCCTAACTATGCATGGGTGGTGACAGAGTTCTTGGGCACAACACTCAAGGAGTGGCTCCATGGCCCTGGCAACAGGCAAAGAGAGAGGATGATTCCACTTCCTCTGTTGAAAGAACGGTTGGATCGGGCATTGGAGATTTCTCAAGCCATGCAGTATTTACATGGGCAAAAGCCGAAGGTGATTCACCGCGACCTCAAACCTAGCAACATATTTTTGGACGATGCCATGCATGTAAGGGTTGCGGATTTTGGTCACGCTCGGTTCTTGAGTGACGAAGAGAAGGCCCTAACTGGTGAAACAGGAACTTATGTGTACATGGCACCAGAGGTGATCCAATGCGAGCCTTATAATGAAAAATGCGATGTGTACAGTTTTGGCATAATACTCAATGAGCTTTTAACTGGAAATTATCCATACATTGAGACAGATTATGGTCCCACCAAGATTGCGATGGAAGTTGTGGAGGCTAACCTAAGGCCAAGGCTTCCTGATGATGATGATGGGCAATTTCGGGAGCTAATTGACCTAATTTGCCTTTCATGGGATGGAGATGCTTCTGTGAGACCATCTTTTGCCACCATTACTTACAGTCTCAAGGATCTTCAAAACAGAATATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

440

Amino Acids

49.69

Weight (kDa)

5.52

Isoelectric Point (pI)

39.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014670)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g31250
malus_domestica MD08G1069800.v1.1 MD15G1052200.v1.1
prunus_persica Prupe.1G406200_v2.0.a1 Prupe.1G406200_v2.0.a1
pyrus_communis pycom15g05000
rosa_chinensis RchiOBHm_Chr6g0311551
rosa_laevigata RLG00000010394
rosa_multiflora Rmu_co8269907.1_g000001 Rmu_sc0007556.1_g000001
rosa_roxburghii Rroxscaffold_7G00157500
rosa_rugosa Rorug06G0389700
rosa_samantha Rh6AG504600 Rh6BG515300 Rh6CG521200 Rh6DG507100
rosa_wichuraiana Rw6G043940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1047
Acc36I ACCTGC 1 cut(s) 175
AccB1I GGYRCC 1 cut(s) 1018
AccII CGCG 2 cut(s) 29, 888
AccIII TCCGGA 1 cut(s) 481
AciI CCGC 4 cut(s) 24, 233, 886, 941
AclI AACGTT 1 cut(s) 138
AclWI GGATC 5 cut(s) 438, 689, 828, 1024, 1305
AcsI RAATTY 2 cut(s) 160, 494
AfaI GTAC 5 cut(s) 239, 473, 669, 1013, 1067
AfiI CCNNNNNNNGG 4 cut(s) 289, 353, 427, 1240
AgsI TTSAA 7 cut(s) 165, 200, 494, 596, 674, 808, 1304
AjnI CCWGG 2 cut(s) 352, 763
AjuI GAANNNNNNNTTGG 2 cut(s) 800, 832
AluBI AGCT 7 cut(s) 123, 181, 191, 437, 634, 1093, 1216
AluI AGCT 7 cut(s) 123, 181, 191, 437, 634, 1093, 1216
Alw21I GWGCWC 1 cut(s) 636
Alw26I GTCTC 4 cut(s) 48, 1115, 1255, 1295
AlwI GGATC 5 cut(s) 438, 689, 828, 1024, 1305
Aor13HI TCCGGA 1 cut(s) 481
AoxI GGCC 5 cut(s) 291, 355, 760, 981, 1175
ApeKI GCWGC 3 cut(s) 114, 181, 652
ApoI RAATTY 2 cut(s) 160, 494
AspLEI GCGC 3 cut(s) 29, 657, 686
AspS9I GGNCC 6 cut(s) 292, 313, 344, 761, 982, 1133
AsuHPI GGTGA 5 cut(s) 730, 875, 889, 1004, 1039
AsuII TTCGAA 1 cut(s) 498
AvaII GGWCC 3 cut(s) 313, 344, 1133
AxyI CCTNAGG 1 cut(s) 1172
BaeGI GKGCMC 1 cut(s) 737
BanI GGYRCC 1 cut(s) 1018
BanII GRGCYC 1 cut(s) 636
Bbv12I GWGCWC 1 cut(s) 636
BbvI GCAGC 3 cut(s) 101, 168, 664
BccI CCATC 8 cut(s) 15, 244, 462, 486, 1144, 1193, 1238, 1273
BceAI ACGGC 2 cut(s) 90, 540
BciT130I CCWGG 2 cut(s) 354, 765
BclI TGATCA 1 cut(s) 448
BcoDI GTCTC 4 cut(s) 48, 1115, 1255, 1295
BfaI CTAG 2 cut(s) 434, 900
BfmI CTRYAG 1 cut(s) 182
BfoI RGCGCY 1 cut(s) 658
BfuAI ACCTGC 1 cut(s) 175
BisI GCNGC 4 cut(s) 25, 115, 182, 653
BlsI GCNGC 4 cut(s) 26, 116, 183, 654
Bme1390I CCNGG 2 cut(s) 354, 765
Bme18I GGWCC 3 cut(s) 313, 344, 1133
BmgT120I GGNCC 6 cut(s) 292, 313, 344, 761, 982, 1133
BmiI GGNNCC 4 cut(s) 345, 755, 1020, 1135
BmrFI CCNGG 2 cut(s) 354, 765
BmsI GCATC 3 cut(s) 83, 910, 1240
Bpu14I TTCGAA 1 cut(s) 498
BpuEI CTTGAG 5 cut(s) 103, 728, 825, 985, 1277
BsaI GGTCTC 2 cut(s) 48, 1255
BsaJI CCNNGG 3 cut(s) 757, 763, 1178
BsaWI WCCGGW 1 cut(s) 481
Bsc4I CCNNNNNNNGG 4 cut(s) 289, 353, 427, 1240
Bse118I RCCGGY 1 cut(s) 648
Bse1I ACTGG 2 cut(s) 994, 1105
Bse21I CCTNAGG 1 cut(s) 1172
BseAI TCCGGA 1 cut(s) 481
BseBI CCWGG 2 cut(s) 354, 765
BseDI CCNNGG 3 cut(s) 757, 763, 1178
BseGI GGATG 7 cut(s) 98, 255, 473, 474, 478, 792, 1249
BseLI CCNNNNNNNGG 4 cut(s) 289, 353, 427, 1240
BseMII CTCAG 2 cut(s) 62, 263
BseNI ACTGG 2 cut(s) 994, 1105
BseRI GAGGAG 2 cut(s) 36, 402
BseSI GKGCMC 1 cut(s) 737
BseXI GCAGC 3 cut(s) 101, 168, 664
Bsh1236I CGCG 2 cut(s) 29, 888
BshFI GGCC 5 cut(s) 293, 357, 762, 983, 1177
BshNI GGYRCC 1 cut(s) 1018
BsiHKAI GWGCWC 1 cut(s) 636
BsiSI CCGG 2 cut(s) 482, 649
BslFI GGGAC 1 cut(s) 1119
BslI CCNNNNNNNGG 4 cut(s) 289, 353, 427, 1240
BsmAI GTCTC 4 cut(s) 48, 1115, 1255, 1295
BsmFI GGGAC 1 cut(s) 1119
BsnI GGCC 5 cut(s) 293, 357, 762, 983, 1177
Bso31I GGTCTC 2 cut(s) 48, 1255
Bsp119I TTCGAA 1 cut(s) 498
Bsp1286I GDGCHC 2 cut(s) 636, 737
Bsp13I TCCGGA 1 cut(s) 481
Bsp1407I TGTACA 3 cut(s) 237, 1011, 1065
Bsp143I GATC 6 cut(s) 430, 448, 694, 820, 1029, 1297
Bsp19I CCATGG 1 cut(s) 757
BspACI CCGC 4 cut(s) 24, 233, 886, 941
BspANI GGCC 5 cut(s) 293, 357, 762, 983, 1177
BspCNI CTCAG 2 cut(s) 61, 264
BspEI TCCGGA 1 cut(s) 481
BspFNI CGCG 2 cut(s) 29, 888
BspLI GGNNCC 4 cut(s) 345, 755, 1020, 1135
BspMAI CTGCAG 1 cut(s) 186
BspMI ACCTGC 1 cut(s) 175
BspPI GGATC 5 cut(s) 438, 689, 828, 1024, 1305
BspT104I TTCGAA 1 cut(s) 498
BspT107I GGYRCC 1 cut(s) 1018
BspTNI GGTCTC 2 cut(s) 48, 1255
BsrFI RCCGGY 1 cut(s) 648
BsrGI TGTACA 3 cut(s) 237, 1011, 1065
BsrI ACTGG 2 cut(s) 994, 1105
BssAI RCCGGY 1 cut(s) 648
BssECI CCNNGG 3 cut(s) 757, 763, 1178
BssMI GATC 6 cut(s) 430, 448, 694, 820, 1029, 1297
BssT1I CCWWGG 2 cut(s) 757, 1178
Bst2UI CCWGG 2 cut(s) 354, 765
Bst4CI ACNGT 5 cut(s) 426, 611, 816, 1070, 1289
Bst6I CTCTTC 4 cut(s) 239, 480, 495, 969
BstAUI TGTACA 3 cut(s) 237, 1011, 1065
BstBI TTCGAA 1 cut(s) 498
BstC8I GCNNGC 4 cut(s) 69, 650, 688, 1040
BstDEI CTNAG 3 cut(s) 48, 272, 1172
BstDSI CCRYGG 1 cut(s) 757
BstF5I GGATG 7 cut(s) 98, 255, 473, 474, 478, 792, 1249
BstFNI CGCG 2 cut(s) 29, 888
BstH2I RGCGCY 1 cut(s) 658
BstHHI GCGC 3 cut(s) 29, 657, 686
BstKTI GATC 6 cut(s) 433, 451, 697, 823, 1032, 1300
BstMAI GTCTC 4 cut(s) 48, 1115, 1255, 1295
BstMBI GATC 6 cut(s) 430, 448, 694, 820, 1029, 1297
BstMWI GCNNNNNNNGC 3 cut(s) 68, 120, 654
BstNI CCWGG 2 cut(s) 354, 765
BstNSI RCATGY 2 cut(s) 690, 932
BstSCI CCNGG 2 cut(s) 352, 763
BstSFI CTRYAG 1 cut(s) 182
BstSLI GKGCMC 1 cut(s) 737
BstUI CGCG 2 cut(s) 29, 888
BstV1I GCAGC 3 cut(s) 101, 168, 664
BstX2I RGATCY 2 cut(s) 430, 1297
BstXI CCANNNNNNTGG 2 cut(s) 302, 764
BstYI RGATCY 2 cut(s) 430, 1297
Bsu36I CCTNAGG 1 cut(s) 1172
BsuRI GGCC 5 cut(s) 293, 357, 762, 983, 1177
BtgI CCRYGG 1 cut(s) 757
BtgZI GCGATG 2 cut(s) 1074, 1163
BtsCI GGATG 7 cut(s) 98, 255, 473, 474, 478, 792, 1249
BtsI GCAGTG 1 cut(s) 64
BtsIMutI CAGTG 1 cut(s) 64
BveI ACCTGC 1 cut(s) 175
Cac8I GCNNGC 4 cut(s) 69, 650, 688, 1040
CfoI GCGC 3 cut(s) 29, 657, 686
Cfr10I RCCGGY 1 cut(s) 648
Cfr13I GGNCC 6 cut(s) 292, 313, 344, 761, 982, 1133
Csp6I GTAC 5 cut(s) 238, 472, 668, 1012, 1066
CspCI CAANNNNNGTGG 2 cut(s) 1126, 1161
CviQI GTAC 5 cut(s) 238, 472, 668, 1012, 1066
DdeI CTNAG 3 cut(s) 48, 272, 1172
DpnI GATC 6 cut(s) 432, 450, 696, 822, 1031, 1299
DpnII GATC 6 cut(s) 430, 448, 694, 820, 1029, 1297
Eam1104I CTCTTC 4 cut(s) 239, 480, 495, 969
EarI CTCTTC 4 cut(s) 239, 480, 495, 969
Ecl136II GAGCTC 1 cut(s) 634
Eco130I CCWWGG 2 cut(s) 757, 1178
Eco147I AGGCCT 1 cut(s) 357
Eco24I GRGCYC 1 cut(s) 636
Eco31I GGTCTC 2 cut(s) 48, 1255
Eco47I GGWCC 3 cut(s) 313, 344, 1133
Eco53kI GAGCTC 1 cut(s) 634
Eco81I CCTNAGG 1 cut(s) 1172
EcoICRI GAGCTC 1 cut(s) 634
EcoO109I RGGNCCY 1 cut(s) 982
EcoRI GAATTC 1 cut(s) 494
EcoRII CCWGG 2 cut(s) 352, 763
EcoT14I CCWWGG 2 cut(s) 757, 1178
EcoT22I ATGCAT 3 cut(s) 578, 712, 930
EcoT38I GRGCYC 1 cut(s) 636
ErhI CCWWGG 2 cut(s) 757, 1178
FaqI GGGAC 1 cut(s) 1119
FbaI TGATCA 1 cut(s) 448
Fnu4HI GCNGC 4 cut(s) 25, 115, 182, 653
FokI GGATG 7 cut(s) 105, 262, 461, 465, 480, 799, 1256
FriOI GRGCYC 1 cut(s) 636
Fsp4HI GCNGC 4 cut(s) 25, 115, 182, 653
FspBI CTAG 2 cut(s) 434, 900
GlaI GCGC 3 cut(s) 28, 656, 685
GluI GCNGC 4 cut(s) 25, 115, 182, 653
HaeII RGCGCY 1 cut(s) 658
HaeIII GGCC 5 cut(s) 293, 357, 762, 983, 1177
HapII CCGG 2 cut(s) 482, 649
HhaI GCGC 3 cut(s) 29, 657, 686
Hin6I GCGC 3 cut(s) 27, 655, 684
HinP1I GCGC 3 cut(s) 27, 655, 684
HindIII AAGCTT 1 cut(s) 121
HinfI GANTC 5 cut(s) 106, 306, 596, 790, 880
HpaII CCGG 2 cut(s) 482, 649
HphI GGTGA 5 cut(s) 730, 875, 889, 1004, 1039
Hpy166II GTNNAC 3 cut(s) 238, 1012, 1066
Hpy188I TCNGA 3 cut(s) 12, 273, 430
Hpy188III TCNNGA 5 cut(s) 347, 482, 964, 1187, 1211
Hpy8I GTNNAC 3 cut(s) 238, 1012, 1066
Hpy99I CGWCG 2 cut(s) 76, 430
HpyAV CCTTC 3 cut(s) 368, 868, 973
HpyCH4III ACNGT 5 cut(s) 426, 611, 816, 1070, 1289
HpyCH4IV ACGT 1 cut(s) 138
HpyCH4V TGCA 6 cut(s) 62, 184, 576, 710, 850, 928
HpyF10VI GCNNNNNNNGC 3 cut(s) 68, 120, 654
HpyF3I CTNAG 3 cut(s) 48, 272, 1172
HpySE526I ACGT 1 cut(s) 138
HspAI GCGC 3 cut(s) 27, 655, 684
Kpn2I TCCGGA 1 cut(s) 481
KroI GCCGGC 1 cut(s) 648
KroNI GCCGGC 1 cut(s) 650
Ksp22I TGATCA 1 cut(s) 448
Kzo9I GATC 6 cut(s) 430, 448, 694, 820, 1029, 1297
LmnI GCTCC 3 cut(s) 178, 759, 1213
Lsp1109I GCAGC 3 cut(s) 101, 168, 664
LweI GCATC 3 cut(s) 83, 910, 1240
MaeI CTAG 2 cut(s) 434, 900
MaeII ACGT 1 cut(s) 138
MaeIII GTNAC 3 cut(s) 718, 950, 968
MalI GATC 6 cut(s) 432, 450, 696, 822, 1031, 1299
MboI GATC 6 cut(s) 430, 448, 694, 820, 1029, 1297
MboII GAAGA 6 cut(s) 25, 256, 497, 512, 986, 1292
MflI RGATCY 2 cut(s) 430, 1297
MhlI GDGCHC 2 cut(s) 636, 737
MluCI AATT 8 cut(s) 132, 160, 173, 494, 1105, 1205, 1218, 1227
MmeI TCCRAC 2 cut(s) 408, 798
Mph1103I ATGCAT 3 cut(s) 578, 712, 930
MroI TCCGGA 1 cut(s) 481
MroNI GCCGGC 1 cut(s) 648
MseI TTAA 2 cut(s) 135, 1097
MslI CAYNNNNRTG 1 cut(s) 663
MspI CCGG 2 cut(s) 482, 649
MspR9I CCNGG 2 cut(s) 354, 765
MvaI CCWGG 2 cut(s) 354, 765
MvnI CGCG 2 cut(s) 29, 888
MwoI GCNNNNNNNGC 3 cut(s) 68, 120, 654
NaeI GCCGGC 1 cut(s) 650
NcoI CCATGG 1 cut(s) 757
NdeII GATC 6 cut(s) 430, 448, 694, 820, 1029, 1297
NgoMIV GCCGGC 1 cut(s) 648
NlaIV GGNNCC 4 cut(s) 345, 755, 1020, 1135
NmuCI GTSAC 3 cut(s) 718, 950, 968
NsiI ATGCAT 3 cut(s) 578, 712, 930
NspI RCATGY 2 cut(s) 690, 932
NspV TTCGAA 1 cut(s) 498
PaeI GCATGC 1 cut(s) 690
PceI AGGCCT 1 cut(s) 357
PdiI GCCGGC 1 cut(s) 650
PfeI GAWTC 5 cut(s) 106, 306, 596, 790, 880
PkrI GCNGC 4 cut(s) 26, 116, 183, 654
PsiI TTATAA 1 cut(s) 1047
Psp124BI GAGCTC 1 cut(s) 636
Psp1406I AACGTT 1 cut(s) 138
Psp6I CCWGG 2 cut(s) 352, 763
PspGI CCWGG 2 cut(s) 352, 763
PspN4I GGNNCC 4 cut(s) 345, 755, 1020, 1135
PspPI GGNCC 6 cut(s) 292, 313, 344, 761, 982, 1133
PstI CTGCAG 1 cut(s) 186
PsuI RGATCY 2 cut(s) 430, 1297
RsaI GTAC 5 cut(s) 239, 473, 669, 1013, 1067
RsaNI GTAC 5 cut(s) 238, 472, 668, 1012, 1066
RseI CAYNNNNRTG 1 cut(s) 663
SacI GAGCTC 1 cut(s) 636
SaqAI TTAA 2 cut(s) 135, 1097
SatI GCNGC 4 cut(s) 25, 115, 182, 653
Sau3AI GATC 6 cut(s) 430, 448, 694, 820, 1029, 1297
Sau96I GGNCC 6 cut(s) 292, 313, 344, 761, 982, 1133
ScrFI CCNGG 2 cut(s) 354, 765
SduI GDGCHC 2 cut(s) 636, 737
SfaNI GCATC 3 cut(s) 83, 910, 1240
SfcI CTRYAG 1 cut(s) 182
SfuI TTCGAA 1 cut(s) 498
SinI GGWCC 3 cut(s) 313, 344, 1133
SmiMI CAYNNNNRTG 1 cut(s) 663
SmlI CTYRAG 5 cut(s) 118, 743, 840, 964, 1292
SmoI CTYRAG 5 cut(s) 118, 743, 840, 964, 1292
SphI GCATGC 1 cut(s) 690
Sse9I AATT 8 cut(s) 132, 160, 173, 494, 1105, 1205, 1218, 1227
SseBI AGGCCT 1 cut(s) 357
SsiI CCGC 4 cut(s) 24, 233, 886, 941
SspI AATATT 2 cut(s) 373, 1314
SspMI CTAG 2 cut(s) 434, 900
SstI GAGCTC 1 cut(s) 636
StuI AGGCCT 1 cut(s) 357
StyD4I CCNGG 2 cut(s) 352, 763
StyI CCWWGG 2 cut(s) 757, 1178
TaaI ACNGT 5 cut(s) 426, 611, 816, 1070, 1289
TaiI ACGT 1 cut(s) 141
TaqI TCGA 6 cut(s) 53, 397, 498, 599, 636, 693
TasI AATT 8 cut(s) 132, 160, 173, 494, 1105, 1205, 1218, 1227
TatI WGTACW 4 cut(s) 237, 667, 1011, 1065
TauI GCSGC 1 cut(s) 27
TfiI GAWTC 5 cut(s) 106, 306, 596, 790, 880
Tru1I TTAA 2 cut(s) 135, 1097
Tru9I TTAA 2 cut(s) 135, 1097
TscAI CASTG 1 cut(s) 64
TseFI GTSAC 3 cut(s) 718, 950, 968
TseI GCWGC 3 cut(s) 114, 181, 652
Tsp45I GTSAC 3 cut(s) 718, 950, 968
TspDTI ATGAA 4 cut(s) 17, 98, 1065, 1227
TspGWI ACGGA 1 cut(s) 480
TspRI CASTG 1 cut(s) 64
VpaK11BI GGWCC 3 cut(s) 313, 344, 1133
XapI RAATTY 2 cut(s) 160, 494
XceI RCATGY 2 cut(s) 690, 932
XcmI CCANNNNNNNNNTGG 1 cut(s) 1147
XspI CTAG 2 cut(s) 434, 900
Zsp2I ATGCAT 3 cut(s) 578, 712, 930
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.