FvH4_2g31810

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002925
Physical Location & Seq
Reverse (-)
24296642 .. 24300052
3411 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g31810.t1

Sequence Viewer

Length: 819 bp
ATGAAGATGGTGTGGATGGTTACTCGACAGCTTTCGATTAAAGGAAAGAGAAGGCGCATATGGGGAGGAGGAGCTTTTCTCTGTTGGGTTTTGTTAATGTTGGTCACTCCCAAGATTTCTCACTCCCCAAATCACCATCACTATGCTGACATGCGCAACTTCCTTGGGGTGCCCAACACATTGAATGTGATCACCAATTTCCCATTCTTGGTTGTTGGAGTTCTGGGTTTTGTTCTCTGCCTTCAAGGGGGGTTTTTCAATATCAGTTTGCCAGGTGAAGTTTGGGGTTGGGCACTGTTCTATGCAGGAATAGCAGGGTTGGCTTTTGGGTCTGCTTATTATCATTTGAAGCCTGATGATAGTAGAGTGACTTGGGATACCTTGCCGATGATGATCACATTATCCTCGCTTTTCTCTAGTTTTTTAGTCGAGAGAGTGGGAGAGAGAATCGGCTTAAGTTGTCTATTTGCACTCCTCTTCATTGCATTTCTCAGTACTGCCTATGAAAGGACATGTAATGATATTAGATTGTACATGATGTTCGAATTGATTCCGTGTATAGCAATTCCAGGAATGACATTTGTGTTCCCACCCAAATATACTCACTCAAGATATTGGCTTTGGGCAGCAGGGGCTTACATTCTCTCCAAATTTGAAGCCTTTGCTGACATGAAAATATACCATGCAAACCAGTACACTATCAGTGGGCACTCTTTGGAGCATCTATGTTTAGTAATCGTCCCTGTTCTTCTCAGTATTATGCTCATGCACAGAACCAGGAAATGTCAAAGAATAGGCCCCTTTAAAGAATGTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ceramidase

Protein Analysis

273

Amino Acids

31.04

Weight (kDa)

9.31

Isoelectric Point (pI)

37.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ceramidase PF05875 60 - 175 1.1e-06 Ceramidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 155
AccB1I GGYRCC 1 cut(s) 169
AcsI RAATTY 1 cut(s) 650
AfaI GTAC 3 cut(s) 496, 533, 695
AfiI CCNNNNNNNGG 3 cut(s) 208, 247, 507
AflII CTTAAG 1 cut(s) 454
AflIII ACRYGT 1 cut(s) 512
AgsI TTSAA 5 cut(s) 184, 245, 259, 349, 656
AjnI CCWGG 3 cut(s) 271, 568, 776
AjuI GAANNNNNNNTTGG 2 cut(s) 188, 220
AluBI AGCT 2 cut(s) 31, 74
AluI AGCT 2 cut(s) 31, 74
AoxI GGCC 1 cut(s) 796
ApeKI GCWGC 1 cut(s) 626
ApoI RAATTY 1 cut(s) 650
Asp700I GAANNNNTTC 1 cut(s) 549
AspLEI GCGC 2 cut(s) 57, 156
AspS9I GGNCC 1 cut(s) 797
AsuHPI GGTGA 3 cut(s) 125, 184, 287
AsuII TTCGAA 1 cut(s) 543
BaeGI GKGCMC 3 cut(s) 174, 295, 711
BanI GGYRCC 1 cut(s) 169
BbvI GCAGC 1 cut(s) 638
BccI CCATC 2 cut(s) 10, 144
BciT130I CCWGG 3 cut(s) 273, 570, 778
BciVI GTATCC 1 cut(s) 370
BclI TGATCA 2 cut(s) 189, 393
BfaI CTAG 1 cut(s) 417
BfrI CTTAAG 1 cut(s) 454
BfuI GTATCC 1 cut(s) 370
BisI GCNGC 1 cut(s) 627
BlsI GCNGC 1 cut(s) 628
BmcAI AGTACT 1 cut(s) 496
Bme1390I CCNGG 3 cut(s) 273, 570, 778
BmgT120I GGNCC 1 cut(s) 797
BmiI GGNNCC 2 cut(s) 171, 799
BmrFI CCNGG 3 cut(s) 273, 570, 778
BmsI GCATC 1 cut(s) 730
BplI GAGNNNNNCTC 2 cut(s) 63, 95
Bpu14I TTCGAA 1 cut(s) 543
BpuEI CTTGAG 1 cut(s) 592
BsaBI GATNNNNATC 1 cut(s) 392
BsaJI CCNNGG 1 cut(s) 163
BsaXI ACNNNNNCTCC 2 cut(s) 629, 659
Bsc4I CCNNNNNNNGG 3 cut(s) 208, 247, 507
Bse1I ACTGG 1 cut(s) 691
Bse3DI GCAATG 1 cut(s) 480
Bse8I GATNNNNATC 1 cut(s) 392
BseBI CCWGG 3 cut(s) 273, 570, 778
BseDI CCNNGG 1 cut(s) 163
BseGI GGATG 1 cut(s) 21
BseJI GATNNNNATC 1 cut(s) 392
BseLI CCNNNNNNNGG 3 cut(s) 208, 247, 507
BseMI GCAATG 1 cut(s) 480
BseMII CTCAG 2 cut(s) 505, 766
BseNI ACTGG 1 cut(s) 691
BseRI GAGGAG 3 cut(s) 81, 84, 464
BseSI GKGCMC 3 cut(s) 174, 295, 711
BseXI GCAGC 1 cut(s) 638
BshFI GGCC 1 cut(s) 798
BshNI GGYRCC 1 cut(s) 169
BslFI GGGAC 1 cut(s) 725
BslI CCNNNNNNNGG 3 cut(s) 208, 247, 507
BsmFI GGGAC 1 cut(s) 725
BsnI GGCC 1 cut(s) 798
Bsp119I TTCGAA 1 cut(s) 543
Bsp1286I GDGCHC 3 cut(s) 174, 295, 711
Bsp1407I TGTACA 1 cut(s) 531
Bsp143I GATC 2 cut(s) 189, 393
BspANI GGCC 1 cut(s) 798
BspCNI CTCAG 2 cut(s) 504, 765
BspLI GGNNCC 2 cut(s) 171, 799
BspT104I TTCGAA 1 cut(s) 543
BspT107I GGYRCC 1 cut(s) 169
BspTI CTTAAG 1 cut(s) 454
BsrDI GCAATG 1 cut(s) 480
BsrGI TGTACA 1 cut(s) 531
BsrI ACTGG 1 cut(s) 691
BssECI CCNNGG 1 cut(s) 163
BssMI GATC 2 cut(s) 189, 393
BssT1I CCWWGG 1 cut(s) 163
Bst2UI CCWGG 3 cut(s) 273, 570, 778
Bst4CI ACNGT 1 cut(s) 297
Bst6I CTCTTC 1 cut(s) 482
BstAFI CTTAAG 1 cut(s) 454
BstAUI TGTACA 1 cut(s) 531
BstBI TTCGAA 1 cut(s) 543
BstDEI CTNAG 2 cut(s) 491, 752
BstENI CCTNNNNNAGG 1 cut(s) 505
BstF5I GGATG 1 cut(s) 21
BstHHI GCGC 2 cut(s) 57, 156
BstKTI GATC 2 cut(s) 192, 396
BstMBI GATC 2 cut(s) 189, 393
BstMWI GCNNNNNNNGC 3 cut(s) 311, 320, 632
BstNI CCWGG 3 cut(s) 273, 570, 778
BstNSI RCATGY 2 cut(s) 154, 516
BstSCI CCNGG 3 cut(s) 271, 568, 776
BstSLI GKGCMC 3 cut(s) 174, 295, 711
BstV1I GCAGC 1 cut(s) 638
BsuI GTATCC 1 cut(s) 370
BsuRI GGCC 1 cut(s) 798
BtsCI GGATG 1 cut(s) 21
BtsIMutI CAGTG 2 cut(s) 293, 709
CfoI GCGC 2 cut(s) 57, 156
Cfr13I GGNCC 1 cut(s) 797
Csp6I GTAC 3 cut(s) 495, 532, 694
CviAII CATG 6 cut(s) 151, 513, 535, 670, 683, 766
CviJI RGCY 9 cut(s) 31, 74, 323, 352, 453, 619, 635, 659, 798
CviKI_1 RGCY 9 cut(s) 31, 74, 323, 352, 453, 619, 635, 659, 798
CviQI GTAC 3 cut(s) 495, 532, 694
DdeI CTNAG 2 cut(s) 491, 752
DpnI GATC 2 cut(s) 191, 395
DpnII GATC 2 cut(s) 189, 393
DraI TTTAAA 1 cut(s) 805
Eam1104I CTCTTC 1 cut(s) 482
EarI CTCTTC 1 cut(s) 482
Eco130I CCWWGG 1 cut(s) 163
EcoNI CCTNNNNNAGG 1 cut(s) 505
EcoO109I RGGNCCY 1 cut(s) 797
EcoRII CCWGG 3 cut(s) 271, 568, 776
EcoT14I CCWWGG 1 cut(s) 163
ErhI CCWWGG 1 cut(s) 163
FaeI CATG 6 cut(s) 154, 516, 538, 673, 686, 769
FaqI GGGAC 1 cut(s) 725
FatI CATG 6 cut(s) 150, 512, 534, 669, 682, 765
FauNDI CATATG 1 cut(s) 59
FbaI TGATCA 2 cut(s) 189, 393
Fnu4HI GCNGC 1 cut(s) 627
FokI GGATG 1 cut(s) 28
Fsp4HI GCNGC 1 cut(s) 627
FspBI CTAG 1 cut(s) 417
FspI TGCGCA 1 cut(s) 155
GlaI GCGC 2 cut(s) 56, 155
GluI GCNGC 1 cut(s) 627
HaeIII GGCC 1 cut(s) 798
HhaI GCGC 2 cut(s) 57, 156
Hin1II CATG 6 cut(s) 154, 516, 538, 673, 686, 769
Hin6I GCGC 2 cut(s) 55, 154
HinP1I GCGC 2 cut(s) 55, 154
HinfI GANTC 2 cut(s) 447, 550
HphI GGTGA 3 cut(s) 125, 184, 287
Hpy166II GTNNAC 1 cut(s) 696
Hpy188III TCNNGA 3 cut(s) 430, 609, 816
Hpy8I GTNNAC 1 cut(s) 696
HpyAV CCTTC 2 cut(s) 45, 251
HpyCH4III ACNGT 1 cut(s) 297
HpyCH4V TGCA 5 cut(s) 305, 470, 485, 686, 769
HpyF10VI GCNNNNNNNGC 3 cut(s) 311, 320, 632
HpyF3I CTNAG 2 cut(s) 491, 752
Hsp92II CATG 6 cut(s) 154, 516, 538, 673, 686, 769
HspAI GCGC 2 cut(s) 55, 154
Ksp22I TGATCA 2 cut(s) 189, 393
Kzo9I GATC 2 cut(s) 189, 393
LmnI GCTCC 2 cut(s) 71, 718
Lsp1109I GCAGC 1 cut(s) 638
LweI GCATC 1 cut(s) 730
MaeI CTAG 1 cut(s) 417
MaeIII GTNAC 3 cut(s) 19, 103, 367
MalI GATC 2 cut(s) 191, 395
MboI GATC 2 cut(s) 189, 393
MboII GAAGA 3 cut(s) 16, 469, 740
MhlI GDGCHC 3 cut(s) 174, 295, 711
MluCI AATT 4 cut(s) 196, 545, 564, 650
MmeI TCCRAC 1 cut(s) 196
MnlI CCTC 4 cut(s) 59, 62, 415, 485
MroXI GAANNNNTTC 1 cut(s) 549
MseI TTAA 4 cut(s) 39, 95, 455, 804
MslI CAYNNNNRTG 1 cut(s) 141
MspCI CTTAAG 1 cut(s) 454
MspR9I CCNGG 3 cut(s) 273, 570, 778
MvaI CCWGG 3 cut(s) 273, 570, 778
MwoI GCNNNNNNNGC 3 cut(s) 311, 320, 632
NdeI CATATG 1 cut(s) 59
NdeII GATC 2 cut(s) 189, 393
NlaIII CATG 6 cut(s) 154, 516, 538, 673, 686, 769
NlaIV GGNNCC 2 cut(s) 171, 799
NmuCI GTSAC 2 cut(s) 103, 367
NsbI TGCGCA 1 cut(s) 155
NspI RCATGY 2 cut(s) 154, 516
NspV TTCGAA 1 cut(s) 543
PciI ACATGT 1 cut(s) 512
PdmI GAANNNNTTC 1 cut(s) 549
PfeI GAWTC 2 cut(s) 447, 550
PfoI TCCNGGA 1 cut(s) 568
PkrI GCNGC 1 cut(s) 628
PscI ACATGT 1 cut(s) 512
Psp6I CCWGG 3 cut(s) 271, 568, 776
PspGI CCWGG 3 cut(s) 271, 568, 776
PspN4I GGNNCC 2 cut(s) 171, 799
PspPI GGNCC 1 cut(s) 797
PsrI GAACNNNNNNTAC 2 cut(s) 524, 556
RsaI GTAC 3 cut(s) 496, 533, 695
RsaNI GTAC 3 cut(s) 495, 532, 694
RseI CAYNNNNRTG 1 cut(s) 141
SaqAI TTAA 4 cut(s) 39, 95, 455, 804
SatI GCNGC 1 cut(s) 627
Sau3AI GATC 2 cut(s) 189, 393
Sau96I GGNCC 1 cut(s) 797
ScaI AGTACT 1 cut(s) 496
ScrFI CCNGG 3 cut(s) 273, 570, 778
SduI GDGCHC 3 cut(s) 174, 295, 711
SetI ASST 4 cut(s) 33, 76, 277, 383
SfaNI GCATC 1 cut(s) 730
SfuI TTCGAA 1 cut(s) 543
SmiMI CAYNNNNRTG 1 cut(s) 141
SmlI CTYRAG 2 cut(s) 454, 607
SmoI CTYRAG 2 cut(s) 454, 607
Sse9I AATT 4 cut(s) 196, 545, 564, 650
SspMI CTAG 1 cut(s) 417
StyD4I CCNGG 3 cut(s) 271, 568, 776
StyI CCWWGG 1 cut(s) 163
TaaI ACNGT 1 cut(s) 297
TaqI TCGA 4 cut(s) 25, 35, 429, 543
TasI AATT 4 cut(s) 196, 545, 564, 650
TatI WGTACW 3 cut(s) 494, 531, 693
TfiI GAWTC 2 cut(s) 447, 550
Tru1I TTAA 4 cut(s) 39, 95, 455, 804
Tru9I TTAA 4 cut(s) 39, 95, 455, 804
TscAI CASTG 2 cut(s) 300, 709
TseFI GTSAC 2 cut(s) 103, 367
TseI GCWGC 1 cut(s) 626
Tsp45I GTSAC 2 cut(s) 103, 367
TspDTI ATGAA 4 cut(s) 17, 469, 519, 686
TspGWI ACGGA 1 cut(s) 543
TspRI CASTG 2 cut(s) 300, 709
Vha464I CTTAAG 1 cut(s) 454
XagI CCTNNNNNAGG 1 cut(s) 505
XapI RAATTY 1 cut(s) 650
XceI RCATGY 2 cut(s) 154, 516
XcmI CCANNNNNNNNNTGG 1 cut(s) 279
XmnI GAANNNNTTC 1 cut(s) 549
XspI CTAG 1 cut(s) 417
ZrmI AGTACT 1 cut(s) 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.