MD08G1062700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0002925
Physical Location & Seq
Forward (+)
4945973 .. 4948732
2760 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1062700.v1.1.491

Sequence Viewer

Length: 810 bp
ATGGTGTGGATGGCAACTCAGCAGCTTGCAATCAGAGGGAAGAGACGGCGGATATGGGGAGGAGCGTTTCTGTGTTGGGTTTTGTTAATTCTGGTCACTCCCAAGATTCCTCACTCCCCCAAACACCATCTGTACGCCGACATGCGCAATCTTCTGGGAGTGCCTAATACCTTGAACGTGATCACAAATTTCCCCTTTTTGGTTGTTGGCGCTCTGGGTTTTGTGCTCTGCGTGCAAGGGGGGTTTTTCAACATCAGTTTGCCAGGCGAGGTTTGGGGTTGGGCGCTATTCTATGCTGGAACAGCAGGGGTTGCATTTGGGTCTGCTTATTATCATTTGAAGCCTGCCGATAGTAGAGTAGCGTGGGACACCTTGCCGATGATGATTGCATACTCCTCCCTTTTCTCTAGTTTTATAGTGGAGAGACTGGGAGGACGGATCGGTTTAAGCTGTCTGTTTGCACTTCTTTTCATTGCTTTTCTTAGCATTGCATATGACAGAACGTGTAATGATATCAGGTTGTACATGATGTTCCAGTTGATTCCGTGTATAGCAATTCCAACAATGTCATCTGTGTTCCCACCAAAATATACTCACTCAAGATATTGGCTTTGGGCCGCAGGGGTTTACCTTCTCTCCAAATTTGAATCTGTTGCTGACAAAAAAATCTACCGTGCAAATCATTTCATTATCAGCGGACACTCGTTGGAGCATTTGTGTTTAGTGATGGTCCCTGTTCTGCTCAGTATTATGCTCATGAATAGAAAGATTAAATGTCAAAGAATAGACGCCTTTAAAGAATGCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ceramidase

Protein Analysis

270

Amino Acids

30.47

Weight (kDa)

9.55

Isoelectric Point (pI)

35.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ceramidase PF05875 57 - 190 8.8e-07 Ceramidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 146
AciI CCGC 3 cut(s) 49, 618, 696
AclWI GGATC 1 cut(s) 446
AcsI RAATTY 2 cut(s) 187, 641
AcyI GRCGYC 1 cut(s) 789
AfaI GTAC 2 cut(s) 134, 524
AfiI CCNNNNNNNGG 1 cut(s) 199
AflIII ACRYGT 1 cut(s) 503
AgsI TTSAA 4 cut(s) 175, 250, 340, 647
AjnI CCWGG 1 cut(s) 262
AluBI AGCT 2 cut(s) 25, 450
AluI AGCT 2 cut(s) 25, 450
Alw21I GWGCWC 1 cut(s) 228
Alw26I GTCTC 2 cut(s) 37, 418
AlwI GGATC 1 cut(s) 446
AoxI GGCC 1 cut(s) 615
ApeKI GCWGC 1 cut(s) 22
ApoI RAATTY 2 cut(s) 187, 641
AspLEI GCGC 3 cut(s) 147, 212, 286
AspS9I GGNCC 2 cut(s) 615, 730
AvaII GGWCC 1 cut(s) 730
Bbv12I GWGCWC 1 cut(s) 228
BbvI GCAGC 1 cut(s) 34
BccI CCATC 3 cut(s) 4, 135, 721
BceAI ACGGC 1 cut(s) 62
BciT130I CCWGG 1 cut(s) 264
BclI TGATCA 1 cut(s) 180
BcoDI GTCTC 2 cut(s) 37, 418
BfaI CTAG 1 cut(s) 408
BfoI RGCGCY 2 cut(s) 213, 287
BisI GCNGC 2 cut(s) 23, 618
BlsI GCNGC 2 cut(s) 24, 619
Bme1390I CCNGG 1 cut(s) 264
Bme18I GGWCC 1 cut(s) 730
BmgT120I GGNCC 2 cut(s) 615, 730
BmiI GGNNCC 1 cut(s) 732
BmrFI CCNGG 1 cut(s) 264
BmrI ACTGGG 1 cut(s) 437
BmuI ACTGGG 1 cut(s) 437
BpuEI CTTGAG 1 cut(s) 583
BsaHI GRCGYC 1 cut(s) 789
BsaXI ACNNNNNCTCC 4 cut(s) 620, 650, 701, 731
Bsc4I CCNNNNNNNGG 1 cut(s) 199
Bse1I ACTGG 2 cut(s) 432, 535
Bse3DI GCAATG 2 cut(s) 471, 486
BseBI CCWGG 1 cut(s) 264
BseGI GGATG 1 cut(s) 15
BseLI CCNNNNNNNGG 1 cut(s) 199
BseMI GCAATG 2 cut(s) 471, 486
BseMII CTCAG 2 cut(s) 32, 757
BseNI ACTGG 2 cut(s) 432, 535
BseRI GAGGAG 2 cut(s) 75, 385
BseXI GCAGC 1 cut(s) 34
BshFI GGCC 1 cut(s) 617
BsiHKAI GWGCWC 1 cut(s) 228
BslFI GGGAC 2 cut(s) 380, 716
BslI CCNNNNNNNGG 1 cut(s) 199
BsmAI GTCTC 2 cut(s) 37, 418
BsmBI CGTCTC 1 cut(s) 37
BsmFI GGGAC 2 cut(s) 380, 716
BsmI GAATGC 1 cut(s) 806
BsnI GGCC 1 cut(s) 617
Bsp1286I GDGCHC 1 cut(s) 228
Bsp1407I TGTACA 1 cut(s) 522
Bsp143I GATC 2 cut(s) 180, 438
BspACI CCGC 3 cut(s) 49, 618, 696
BspANI GGCC 1 cut(s) 617
BspCNI CTCAG 2 cut(s) 31, 756
BspHI TCATGA 1 cut(s) 756
BspLI GGNNCC 1 cut(s) 732
BspPI GGATC 1 cut(s) 446
BsrDI GCAATG 2 cut(s) 471, 486
BsrGI TGTACA 1 cut(s) 522
BsrI ACTGG 2 cut(s) 432, 535
BssMI GATC 2 cut(s) 180, 438
BssNI GRCGYC 1 cut(s) 789
Bst2UI CCWGG 1 cut(s) 264
Bst4CI ACNGT 1 cut(s) 674
Bst6I CTCTTC 1 cut(s) 35
BstACI GRCGYC 1 cut(s) 789
BstAPI GCANNNNNTGC 1 cut(s) 311
BstAUI TGTACA 1 cut(s) 522
BstC8I GCNNGC 3 cut(s) 27, 233, 345
BstDEI CTNAG 3 cut(s) 18, 482, 743
BstF5I GGATG 1 cut(s) 15
BstH2I RGCGCY 2 cut(s) 213, 287
BstHHI GCGC 3 cut(s) 147, 212, 286
BstKTI GATC 2 cut(s) 183, 441
BstMAI GTCTC 2 cut(s) 37, 418
BstMBI GATC 2 cut(s) 180, 438
BstMWI GCNNNNNNNGC 3 cut(s) 232, 302, 311
BstNI CCWGG 1 cut(s) 264
BstNSI RCATGY 1 cut(s) 145
BstSCI CCNGG 1 cut(s) 262
BstV1I GCAGC 1 cut(s) 34
BsuRI GGCC 1 cut(s) 617
BtsCI GGATG 1 cut(s) 15
Cac8I GCNNGC 3 cut(s) 27, 233, 345
CciI TCATGA 1 cut(s) 756
CfoI GCGC 3 cut(s) 147, 212, 286
Cfr13I GGNCC 2 cut(s) 615, 730
CseI GACGC 1 cut(s) 797
Csp6I GTAC 2 cut(s) 133, 523
CviAII CATG 3 cut(s) 142, 526, 757
CviJI RGCY 5 cut(s) 25, 343, 450, 610, 617
CviKI_1 RGCY 5 cut(s) 25, 343, 450, 610, 617
CviQI GTAC 2 cut(s) 133, 523
DdeI CTNAG 3 cut(s) 18, 482, 743
DpnI GATC 2 cut(s) 182, 440
DpnII GATC 2 cut(s) 180, 438
DraI TTTAAA 1 cut(s) 796
Eam1104I CTCTTC 1 cut(s) 35
EarI CTCTTC 1 cut(s) 35
EciI GGCGGA 1 cut(s) 64
Eco32I GATATC 1 cut(s) 514
Eco47I GGWCC 1 cut(s) 730
EcoRII CCWGG 1 cut(s) 262
EcoRV GATATC 1 cut(s) 514
Esp3I CGTCTC 1 cut(s) 37
FaeI CATG 3 cut(s) 145, 529, 760
FaqI GGGAC 2 cut(s) 380, 716
FatI CATG 3 cut(s) 141, 525, 756
FauNDI CATATG 1 cut(s) 493
FbaI TGATCA 1 cut(s) 180
Fnu4HI GCNGC 2 cut(s) 23, 618
FokI GGATG 1 cut(s) 22
Fsp4HI GCNGC 2 cut(s) 23, 618
FspBI CTAG 1 cut(s) 408
FspI TGCGCA 1 cut(s) 146
GlaI GCGC 3 cut(s) 146, 211, 285
GluI GCNGC 2 cut(s) 23, 618
HaeII RGCGCY 2 cut(s) 213, 287
HaeIII GGCC 1 cut(s) 617
HgaI GACGC 1 cut(s) 797
HhaI GCGC 3 cut(s) 147, 212, 286
Hin1I GRCGYC 1 cut(s) 789
Hin1II CATG 3 cut(s) 145, 529, 760
Hin6I GCGC 3 cut(s) 145, 210, 284
HinP1I GCGC 3 cut(s) 145, 210, 284
HinfI GANTC 3 cut(s) 106, 541, 647
Hpy166II GTNNAC 1 cut(s) 628
Hpy188I TCNGA 1 cut(s) 35
Hpy188III TCNNGA 2 cut(s) 600, 757
Hpy8I GTNNAC 1 cut(s) 628
HpyAV CCTTC 1 cut(s) 641
HpyCH4III ACNGT 1 cut(s) 674
HpyCH4IV ACGT 2 cut(s) 177, 503
HpyCH4V TGCA 7 cut(s) 29, 235, 314, 389, 461, 491, 677
HpyF10VI GCNNNNNNNGC 3 cut(s) 232, 302, 311
HpyF3I CTNAG 3 cut(s) 18, 482, 743
HpySE526I ACGT 2 cut(s) 177, 503
Hsp92I GRCGYC 1 cut(s) 789
Hsp92II CATG 3 cut(s) 145, 529, 760
HspAI GCGC 3 cut(s) 145, 210, 284
Ksp22I TGATCA 1 cut(s) 180
Kzo9I GATC 2 cut(s) 180, 438
LmnI GCTCC 2 cut(s) 62, 709
Lsp1109I GCAGC 1 cut(s) 34
MaeI CTAG 1 cut(s) 408
MaeII ACGT 2 cut(s) 177, 503
MaeIII GTNAC 1 cut(s) 94
MalI GATC 2 cut(s) 182, 440
MboI GATC 2 cut(s) 180, 438
MboII GAAGA 2 cut(s) 52, 143
MhlI GDGCHC 1 cut(s) 228
MluCI AATT 4 cut(s) 87, 187, 555, 641
MmeI TCCRAC 2 cut(s) 584, 687
MnlI CCTC 6 cut(s) 29, 53, 120, 262, 406, 425
MseI TTAA 4 cut(s) 86, 446, 771, 795
MspA1I CMGCKG 1 cut(s) 696
MspR9I CCNGG 1 cut(s) 264
Mva1269I GAATGC 1 cut(s) 806
MvaI CCWGG 1 cut(s) 264
MwoI GCNNNNNNNGC 3 cut(s) 232, 302, 311
NdeI CATATG 1 cut(s) 493
NdeII GATC 2 cut(s) 180, 438
NlaIII CATG 3 cut(s) 145, 529, 760
NlaIV GGNNCC 1 cut(s) 732
NmuCI GTSAC 1 cut(s) 94
NsbI TGCGCA 1 cut(s) 146
NspI RCATGY 1 cut(s) 145
PagI TCATGA 1 cut(s) 756
PctI GAATGC 1 cut(s) 806
PfeI GAWTC 3 cut(s) 106, 541, 647
PkrI GCNGC 2 cut(s) 24, 619
Psp6I CCWGG 1 cut(s) 262
PspGI CCWGG 1 cut(s) 262
PspN4I GGNNCC 1 cut(s) 732
PspPI GGNCC 2 cut(s) 615, 730
PsrI GAACNNNNNNTAC 2 cut(s) 515, 547
RsaI GTAC 2 cut(s) 134, 524
RsaNI GTAC 2 cut(s) 133, 523
SaqAI TTAA 4 cut(s) 86, 446, 771, 795
SatI GCNGC 2 cut(s) 23, 618
Sau3AI GATC 2 cut(s) 180, 438
Sau96I GGNCC 2 cut(s) 615, 730
ScrFI CCNGG 1 cut(s) 264
SduI GDGCHC 1 cut(s) 228
SetI ASST 9 cut(s) 27, 173, 180, 273, 374, 452, 506, 521, 633
SinI GGWCC 1 cut(s) 730
SmlI CTYRAG 1 cut(s) 598
SmoI CTYRAG 1 cut(s) 598
Sse9I AATT 4 cut(s) 87, 187, 555, 641
SsiI CCGC 3 cut(s) 49, 618, 696
SspMI CTAG 1 cut(s) 408
StyD4I CCNGG 1 cut(s) 262
TaaI ACNGT 1 cut(s) 674
TaiI ACGT 2 cut(s) 180, 506
TasI AATT 4 cut(s) 87, 187, 555, 641
TatI WGTACW 1 cut(s) 522
TauI GCSGC 1 cut(s) 620
TfiI GAWTC 3 cut(s) 106, 541, 647
Tru1I TTAA 4 cut(s) 86, 446, 771, 795
Tru9I TTAA 4 cut(s) 86, 446, 771, 795
TseFI GTSAC 1 cut(s) 94
TseI GCWGC 1 cut(s) 22
Tsp45I GTSAC 1 cut(s) 94
TspDTI ATGAA 3 cut(s) 460, 676, 773
TspGWI ACGGA 2 cut(s) 451, 534
VpaK11BI GGWCC 1 cut(s) 730
XapI RAATTY 2 cut(s) 187, 641
XceI RCATGY 1 cut(s) 145
XcmI CCANNNNNNNNNTGG 1 cut(s) 270
XspI CTAG 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.