Rh6CG514900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0002925
Physical Location & Seq
Forward (+)
67290193 .. 67293869
3677 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG514900.1

Sequence Viewer

Length: 792 bp
ATGAGAGTCCATGACAAAAGGGCTCTCTCACCAGAAAAGGGTCGCATGAATTCACATGTATACATATGTGTAAAGCTTAAAGTAACTGCAACAAGAGCAAAGACCTTCCTTTTCTTTCTAGTTCTACACACAGAGAGAGTGAGGAGGAAGATGGTAACTCAACAGTTTGTGATTAAAGGAAAGAGAAGGCGCATATGGGGAGGAGCTTTTCTCTGTTGGGTTCTGTTAATGTTGGTCACTCCCAAAATTTCTCACTCCCCAAATCACCATCACTATGCTGACATGCGCAATTTCCTTGGAGTGCCCAACACATTGAATGTGATCACCAGTTTCCCATTCTTGGTTGTTGGGGTTCTGGGTTTTGTTCTCTGTGTCCAAGGAGGCTTTTTCAATATCAGTTTGCCAGGTGAAGTTTGGGGTTGGGCACTGTTCTATGCAGGAATAGCAGGGCTGGCTTTTGGGTCTGCTTATTATCATTTGAAGCCTGATGATAGTAGAGTGACTTGGGATACCTTGCCGATGATGATCGCATTCTCCTCGCTTTTCTCTAGTTTTATAGTGGAGAGAGCGGGAGAGAGAATTGGCTTAAGTTGTCTATTTGCACTCCTCGTTATTGCGGTTCTTAGTACTGCTTATGAGAGAACATGCAATGATATCAGATTGTACATGATGTTCCAATTGATTCCATGTATAGCAATTCCAGGAATGACATTTGTGTTCCCACCCAAATATACTCACTCAAGATATTGGCTTTGGGCAGCAGGTAGGTCATTCTCATCATTTTTCTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ceramidase

Protein Analysis

263

Amino Acids

30.03

Weight (kDa)

10.03

Isoelectric Point (pI)

36.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ceramidase PF05875 104 - 221 6.1e-07 Ceramidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 287
Acc36I ACCTGC 1 cut(s) 752
AccBSI CCGCTC 1 cut(s) 569
AccI GTMKAC 1 cut(s) 60
AciI CCGC 2 cut(s) 569, 617
AcsI RAATTY 2 cut(s) 49, 246
AfaI GTAC 2 cut(s) 628, 665
AfiI CCNNNNNNNGG 2 cut(s) 38, 340
AflII CTTAAG 1 cut(s) 586
AflIII ACRYGT 1 cut(s) 55
AgsI TTSAA 3 cut(s) 316, 391, 481
AjnI CCWGG 2 cut(s) 403, 700
AluBI AGCT 2 cut(s) 76, 206
AluI AGCT 2 cut(s) 76, 206
ApeKI GCWGC 1 cut(s) 758
ApoI RAATTY 2 cut(s) 49, 246
AspLEI GCGC 2 cut(s) 192, 288
AsuHPI GGTGA 4 cut(s) 21, 257, 316, 419
BaeGI GKGCMC 2 cut(s) 306, 427
BanII GRGCYC 1 cut(s) 25
BbvI GCAGC 1 cut(s) 770
BccI CCATC 2 cut(s) 145, 276
BcgI CGANNNNNNTGC 2 cut(s) 519, 553
BciT130I CCWGG 2 cut(s) 405, 702
BciVI GTATCC 1 cut(s) 502
BclI TGATCA 1 cut(s) 321
BfaI CTAG 2 cut(s) 119, 549
BfrI CTTAAG 1 cut(s) 586
BfuAI ACCTGC 1 cut(s) 752
BfuI GTATCC 1 cut(s) 502
BisI GCNGC 1 cut(s) 759
BlsI GCNGC 1 cut(s) 760
BmcAI AGTACT 1 cut(s) 628
Bme1390I CCNGG 2 cut(s) 405, 702
BmrFI CCNGG 2 cut(s) 405, 702
BplI GAGNNNNNCTC 2 cut(s) 195, 227
BpuEI CTTGAG 1 cut(s) 724
BsaBI GATNNNNATC 1 cut(s) 524
BsaJI CCNNGG 2 cut(s) 295, 376
Bsc4I CCNNNNNNNGG 2 cut(s) 38, 340
Bse1I ACTGG 1 cut(s) 327
Bse3DI GCAATG 1 cut(s) 655
Bse8I GATNNNNATC 1 cut(s) 524
BseBI CCWGG 2 cut(s) 405, 702
BseDI CCNNGG 2 cut(s) 295, 376
BseJI GATNNNNATC 1 cut(s) 524
BseLI CCNNNNNNNGG 2 cut(s) 38, 340
BseMI GCAATG 1 cut(s) 655
BseNI ACTGG 1 cut(s) 327
BseRI GAGGAG 4 cut(s) 157, 216, 526, 596
BseSI GKGCMC 2 cut(s) 306, 427
BseXI GCAGC 1 cut(s) 770
BslI CCNNNNNNNGG 2 cut(s) 38, 340
BsmI GAATGC 1 cut(s) 530
Bsp1286I GDGCHC 3 cut(s) 25, 306, 427
Bsp1407I TGTACA 1 cut(s) 663
Bsp143I GATC 2 cut(s) 321, 525
BspACI CCGC 2 cut(s) 569, 617
BspMI ACCTGC 1 cut(s) 752
BspTI CTTAAG 1 cut(s) 586
BsrBI CCGCTC 1 cut(s) 569
BsrDI GCAATG 1 cut(s) 655
BsrGI TGTACA 1 cut(s) 663
BsrI ACTGG 1 cut(s) 327
BssECI CCNNGG 2 cut(s) 295, 376
BssMI GATC 2 cut(s) 321, 525
BssNAI GTATAC 1 cut(s) 61
BssT1I CCWWGG 2 cut(s) 295, 376
Bst1107I GTATAC 1 cut(s) 61
Bst2UI CCWGG 2 cut(s) 405, 702
Bst4CI ACNGT 2 cut(s) 165, 429
BstAFI CTTAAG 1 cut(s) 586
BstAUI TGTACA 1 cut(s) 663
BstC8I GCNNGC 1 cut(s) 453
BstDEI CTNAG 1 cut(s) 623
BstHHI GCGC 2 cut(s) 192, 288
BstKTI GATC 2 cut(s) 324, 528
BstMBI GATC 2 cut(s) 321, 525
BstMWI GCNNNNNNNGC 3 cut(s) 95, 443, 452
BstNI CCWGG 2 cut(s) 405, 702
BstNSI RCATGY 3 cut(s) 59, 286, 648
BstSCI CCNGG 2 cut(s) 403, 700
BstSLI GKGCMC 2 cut(s) 306, 427
BstV1I GCAGC 1 cut(s) 770
BstZ17I GTATAC 1 cut(s) 61
BsuI GTATCC 1 cut(s) 502
BtsIMutI CAGTG 1 cut(s) 425
BveI ACCTGC 1 cut(s) 752
Cac8I GCNNGC 1 cut(s) 453
CfoI GCGC 2 cut(s) 192, 288
Csp6I GTAC 2 cut(s) 627, 664
CviAII CATG 7 cut(s) 11, 46, 56, 283, 645, 667, 687
CviJI RGCY 9 cut(s) 23, 76, 206, 384, 451, 455, 484, 585, 751
CviKI_1 RGCY 9 cut(s) 23, 76, 206, 384, 451, 455, 484, 585, 751
CviQI GTAC 2 cut(s) 627, 664
DdeI CTNAG 1 cut(s) 623
DpnI GATC 2 cut(s) 323, 527
DpnII GATC 2 cut(s) 321, 525
Eco130I CCWWGG 2 cut(s) 295, 376
Eco24I GRGCYC 1 cut(s) 25
Eco32I GATATC 1 cut(s) 655
EcoRI GAATTC 1 cut(s) 49
EcoRII CCWGG 2 cut(s) 403, 700
EcoRV GATATC 1 cut(s) 655
EcoT14I CCWWGG 2 cut(s) 295, 376
EcoT38I GRGCYC 1 cut(s) 25
ErhI CCWWGG 2 cut(s) 295, 376
FaeI CATG 7 cut(s) 14, 49, 59, 286, 648, 670, 690
FatI CATG 7 cut(s) 10, 45, 55, 282, 644, 666, 686
FauI CCCGC 1 cut(s) 562
FauNDI CATATG 2 cut(s) 65, 194
FbaI TGATCA 1 cut(s) 321
FblI GTMKAC 1 cut(s) 60
Fnu4HI GCNGC 1 cut(s) 759
FriOI GRGCYC 1 cut(s) 25
Fsp4HI GCNGC 1 cut(s) 759
FspBI CTAG 2 cut(s) 119, 549
FspI TGCGCA 1 cut(s) 287
GlaI GCGC 2 cut(s) 191, 287
GluI GCNGC 1 cut(s) 759
HhaI GCGC 2 cut(s) 192, 288
Hin1II CATG 7 cut(s) 14, 49, 59, 286, 648, 670, 690
Hin6I GCGC 2 cut(s) 190, 286
HinP1I GCGC 2 cut(s) 190, 286
HindIII AAGCTT 1 cut(s) 74
HinfI GANTC 2 cut(s) 6, 682
HphI GGTGA 4 cut(s) 21, 257, 316, 419
Hpy166II GTNNAC 1 cut(s) 61
Hpy188I TCNGA 1 cut(s) 659
Hpy188III TCNNGA 2 cut(s) 741, 789
Hpy8I GTNNAC 1 cut(s) 61
HpyAV CCTTC 2 cut(s) 115, 180
HpyCH4III ACNGT 2 cut(s) 165, 429
HpyCH4V TGCA 4 cut(s) 89, 437, 602, 648
HpyF10VI GCNNNNNNNGC 3 cut(s) 95, 443, 452
HpyF3I CTNAG 1 cut(s) 623
Hsp92II CATG 7 cut(s) 14, 49, 59, 286, 648, 670, 690
HspAI GCGC 2 cut(s) 190, 286
Ksp22I TGATCA 1 cut(s) 321
Kzo9I GATC 2 cut(s) 321, 525
LmnI GCTCC 1 cut(s) 203
Lsp1109I GCAGC 1 cut(s) 770
MaeI CTAG 2 cut(s) 119, 549
MaeIII GTNAC 4 cut(s) 82, 154, 235, 499
MalI GATC 2 cut(s) 323, 527
MbiI CCGCTC 1 cut(s) 569
MboI GATC 2 cut(s) 321, 525
MboII GAAGA 1 cut(s) 160
MfeI CAATTG 1 cut(s) 677
MhlI GDGCHC 3 cut(s) 25, 306, 427
MluCI AATT 6 cut(s) 49, 246, 289, 579, 677, 696
MlyI GAGTC 1 cut(s) 15
MnlI CCTC 6 cut(s) 135, 138, 194, 374, 547, 617
MseI TTAA 4 cut(s) 78, 174, 227, 587
MslI CAYNNNNRTG 1 cut(s) 273
MspCI CTTAAG 1 cut(s) 586
MspR9I CCNGG 2 cut(s) 405, 702
MunI CAATTG 1 cut(s) 677
Mva1269I GAATGC 1 cut(s) 530
MvaI CCWGG 2 cut(s) 405, 702
MwoI GCNNNNNNNGC 3 cut(s) 95, 443, 452
NdeI CATATG 2 cut(s) 65, 194
NdeII GATC 2 cut(s) 321, 525
NlaIII CATG 7 cut(s) 14, 49, 59, 286, 648, 670, 690
NmuCI GTSAC 2 cut(s) 235, 499
NsbI TGCGCA 1 cut(s) 287
NspI RCATGY 3 cut(s) 59, 286, 648
PciI ACATGT 1 cut(s) 55
PctI GAATGC 1 cut(s) 530
PfeI GAWTC 1 cut(s) 682
PfoI TCCNGGA 1 cut(s) 700
PkrI GCNGC 1 cut(s) 760
PleI GAGTC 1 cut(s) 14
PpsI GAGTC 1 cut(s) 14
PscI ACATGT 1 cut(s) 55
Psp6I CCWGG 2 cut(s) 403, 700
PspGI CCWGG 2 cut(s) 403, 700
PsrI GAACNNNNNNTAC 2 cut(s) 656, 688
RsaI GTAC 2 cut(s) 628, 665
RsaNI GTAC 2 cut(s) 627, 664
RseI CAYNNNNRTG 1 cut(s) 273
SaqAI TTAA 4 cut(s) 78, 174, 227, 587
SatI GCNGC 1 cut(s) 759
Sau3AI GATC 2 cut(s) 321, 525
ScaI AGTACT 1 cut(s) 628
SchI GAGTC 1 cut(s) 15
ScrFI CCNGG 2 cut(s) 405, 702
SduI GDGCHC 3 cut(s) 25, 306, 427
SetI ASST 7 cut(s) 78, 107, 208, 409, 515, 766, 770
SmiMI CAYNNNNRTG 1 cut(s) 273
SmlI CTYRAG 2 cut(s) 586, 739
SmoI CTYRAG 2 cut(s) 586, 739
Sse9I AATT 6 cut(s) 49, 246, 289, 579, 677, 696
SsiI CCGC 2 cut(s) 569, 617
SspMI CTAG 2 cut(s) 119, 549
StyD4I CCNGG 2 cut(s) 403, 700
StyI CCWWGG 2 cut(s) 295, 376
TaaI ACNGT 2 cut(s) 165, 429
TasI AATT 6 cut(s) 49, 246, 289, 579, 677, 696
TatI WGTACW 2 cut(s) 626, 663
TfiI GAWTC 1 cut(s) 682
Tru1I TTAA 4 cut(s) 78, 174, 227, 587
Tru9I TTAA 4 cut(s) 78, 174, 227, 587
TscAI CASTG 1 cut(s) 432
TseFI GTSAC 2 cut(s) 235, 499
TseI GCWGC 1 cut(s) 758
Tsp45I GTSAC 2 cut(s) 235, 499
TspDTI ATGAA 1 cut(s) 62
TspRI CASTG 1 cut(s) 432
Vha464I CTTAAG 1 cut(s) 586
XapI RAATTY 2 cut(s) 49, 246
XceI RCATGY 3 cut(s) 59, 286, 648
XcmI CCANNNNNNNNNTGG 1 cut(s) 411
XmiI GTMKAC 1 cut(s) 60
XspI CTAG 2 cut(s) 119, 549
ZrmI AGTACT 1 cut(s) 628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.