MD15G1059000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002925
Physical Location & Seq
Reverse (-)
3937617 .. 3940293
2677 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1059000.v1.1.491

Sequence Viewer

Length: 810 bp
ATGGTGTGGATGGCAACTCAGCAGCTTGCAATCAGAGGGAAGAGACGGCGGATATGGGGAGGGGCGCTTCTGTGTTGGGTTTTGTTAATGCTGGTCACTCCCAAGATTTCTCACTCTCCCAAACACCATCTCTACGCTGACATGCGCAATTTTGTGGGAGTGCCCAGTACCTTGAATGTGATCACAAATTTCCCATTTTTGGTTGTTGGCGTTCTGGGTTTTGTGCTCTGCGTGCAAGGGGGGCTTTTTAACATCAGTTTGCCGGGTGAGGTTTGGGGTTGGGCACTATTCTATGCTGGAATGGCAGGGCTGGCATTTGGGTCTGCTTATTATCATTTGAAGCCTGATGATAGTAGAGTAACTTGGGACACCTTGCCGATGATGATTGCATACTCCTCTCTTTTCTCTAGTTTTATAGTGGAGAGAGTAGGAGAGCGGATCGGTTTGAGCTGTCTGTTTGCACTCCTTTTCATTGCTTTTCTTAGCATTGCATATGACAGAACGTATAATGATATCAGGTTGTCCATGATGTTCCAGTTGATTCCATGTATGGCAATTCCAGCAATGTCATTTGTGTTCCCACCTAAATATACTCACTCAAGATATTGGCTTTGGGCCGCTGGGGTTTACCTTCTCGCCAAATTTGAATCCGTTGCCGACGGCAAAATCTACCATGCAAATCGTTTCATTATCAGTGGACACTCGTTGGGGCATTTGTGTTTAGTGATGGTCCCTGTTCTGCTCAGTATCATGCTCATGAATAGAAACATTAAATGTCAAAGAATAGGCGCCTTTAAAGAATGTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ceramidase

Protein Analysis

270

Amino Acids

30.38

Weight (kDa)

9.49

Isoelectric Point (pI)

36.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ceramidase PF05875 58 - 188 4.9e-07 Ceramidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 146
AccB1I GGYRCC 1 cut(s) 788
AccBSI CCGCTC 1 cut(s) 436
AciI CCGC 3 cut(s) 49, 436, 618
AclWI GGATC 1 cut(s) 446
AcsI RAATTY 2 cut(s) 187, 641
AcyI GRCGYC 1 cut(s) 789
AfaI GTAC 1 cut(s) 169
AfiI CCNNNNNNNGG 1 cut(s) 199
AgsI TTSAA 3 cut(s) 175, 340, 647
AluBI AGCT 2 cut(s) 25, 450
AluI AGCT 2 cut(s) 25, 450
Alw21I GWGCWC 1 cut(s) 228
Alw26I GTCTC 1 cut(s) 37
AlwI GGATC 1 cut(s) 446
AoxI GGCC 1 cut(s) 615
ApeKI GCWGC 1 cut(s) 22
ApoI RAATTY 2 cut(s) 187, 641
AspLEI GCGC 3 cut(s) 67, 147, 791
AspS9I GGNCC 2 cut(s) 615, 730
AsuC2I CCSGG 1 cut(s) 264
AsuHPI GGTGA 1 cut(s) 278
AvaII GGWCC 1 cut(s) 730
BaeGI GKGCMC 2 cut(s) 165, 286
BanI GGYRCC 1 cut(s) 788
Bbv12I GWGCWC 1 cut(s) 228
BbvI GCAGC 1 cut(s) 34
BccI CCATC 3 cut(s) 4, 135, 721
BceAI ACGGC 2 cut(s) 62, 676
BclI TGATCA 1 cut(s) 180
BcnI CCSGG 1 cut(s) 264
BcoDI GTCTC 1 cut(s) 37
BfaI CTAG 1 cut(s) 408
BfoI RGCGCY 2 cut(s) 68, 792
BisI GCNGC 2 cut(s) 23, 618
BlsI GCNGC 2 cut(s) 24, 619
Bme1390I CCNGG 1 cut(s) 264
Bme18I GGWCC 1 cut(s) 730
BmgT120I GGNCC 2 cut(s) 615, 730
BmiI GGNNCC 2 cut(s) 732, 790
BmrFI CCNGG 1 cut(s) 264
BmrI ACTGGG 1 cut(s) 159
BmuI ACTGGG 1 cut(s) 159
BpuEI CTTGAG 1 cut(s) 583
BpuMI CCSGG 1 cut(s) 264
BsaHI GRCGYC 1 cut(s) 789
Bsc4I CCNNNNNNNGG 1 cut(s) 199
Bse1I ACTGG 2 cut(s) 165, 535
Bse3DI GCAATG 3 cut(s) 471, 486, 570
BseGI GGATG 1 cut(s) 15
BseLI CCNNNNNNNGG 1 cut(s) 199
BseMI GCAATG 3 cut(s) 471, 486, 570
BseMII CTCAG 2 cut(s) 32, 757
BseNI ACTGG 2 cut(s) 165, 535
BseRI GAGGAG 1 cut(s) 385
BseSI GKGCMC 2 cut(s) 165, 286
BseXI GCAGC 1 cut(s) 34
BseYI CCCAGC 1 cut(s) 620
BshFI GGCC 1 cut(s) 617
BshNI GGYRCC 1 cut(s) 788
BsiHKAI GWGCWC 1 cut(s) 228
BsiSI CCGG 1 cut(s) 263
BslFI GGGAC 2 cut(s) 380, 716
BslI CCNNNNNNNGG 1 cut(s) 199
BsmAI GTCTC 1 cut(s) 37
BsmBI CGTCTC 1 cut(s) 37
BsmFI GGGAC 2 cut(s) 380, 716
BsnI GGCC 1 cut(s) 617
Bsp1286I GDGCHC 3 cut(s) 165, 228, 286
Bsp143I GATC 2 cut(s) 180, 438
BspACI CCGC 3 cut(s) 49, 436, 618
BspANI GGCC 1 cut(s) 617
BspCNI CTCAG 2 cut(s) 31, 756
BspHI TCATGA 2 cut(s) 756, 806
BspLI GGNNCC 2 cut(s) 732, 790
BspPI GGATC 1 cut(s) 446
BspT107I GGYRCC 1 cut(s) 788
BsrBI CCGCTC 1 cut(s) 436
BsrDI GCAATG 3 cut(s) 471, 486, 570
BsrI ACTGG 2 cut(s) 165, 535
BssMI GATC 2 cut(s) 180, 438
BssNI GRCGYC 1 cut(s) 789
Bst6I CTCTTC 1 cut(s) 35
BstACI GRCGYC 1 cut(s) 789
BstC8I GCNNGC 3 cut(s) 27, 233, 312
BstDEI CTNAG 3 cut(s) 18, 482, 743
BstF5I GGATG 1 cut(s) 15
BstH2I RGCGCY 2 cut(s) 68, 792
BstHHI GCGC 3 cut(s) 67, 147, 791
BstKTI GATC 2 cut(s) 183, 441
BstMAI GTCTC 1 cut(s) 37
BstMBI GATC 2 cut(s) 180, 438
BstMWI GCNNNNNNNGC 5 cut(s) 232, 241, 302, 311, 560
BstNSI RCATGY 1 cut(s) 145
BstSCI CCNGG 1 cut(s) 262
BstSLI GKGCMC 2 cut(s) 165, 286
BstV1I GCAGC 1 cut(s) 34
BsuRI GGCC 1 cut(s) 617
BtsCI GGATG 1 cut(s) 15
BtsIMutI CAGTG 1 cut(s) 700
Cac8I GCNNGC 3 cut(s) 27, 233, 312
CciI TCATGA 2 cut(s) 756, 806
CfoI GCGC 3 cut(s) 67, 147, 791
Cfr13I GGNCC 2 cut(s) 615, 730
Csp6I GTAC 1 cut(s) 168
CviAII CATG 7 cut(s) 142, 526, 546, 674, 751, 757, 807
CviJI RGCY 7 cut(s) 25, 244, 310, 343, 450, 610, 617
CviKI_1 RGCY 7 cut(s) 25, 244, 310, 343, 450, 610, 617
CviQI GTAC 1 cut(s) 168
DdeI CTNAG 3 cut(s) 18, 482, 743
DinI GGCGCC 1 cut(s) 790
DpnI GATC 2 cut(s) 182, 440
DpnII GATC 2 cut(s) 180, 438
DraI TTTAAA 1 cut(s) 796
Eam1104I CTCTTC 1 cut(s) 35
EarI CTCTTC 1 cut(s) 35
EciI GGCGGA 1 cut(s) 64
Eco32I GATATC 1 cut(s) 514
Eco47I GGWCC 1 cut(s) 730
EcoRV GATATC 1 cut(s) 514
EgeI GGCGCC 1 cut(s) 790
EheI GGCGCC 1 cut(s) 790
Esp3I CGTCTC 1 cut(s) 37
FaeI CATG 7 cut(s) 145, 529, 549, 677, 754, 760, 810
FalI AAGNNNNNCTT 2 cut(s) 228, 260
FaqI GGGAC 2 cut(s) 380, 716
FatI CATG 7 cut(s) 141, 525, 545, 673, 750, 756, 806
FauNDI CATATG 1 cut(s) 493
FbaI TGATCA 1 cut(s) 180
Fnu4HI GCNGC 2 cut(s) 23, 618
FokI GGATG 1 cut(s) 22
Fsp4HI GCNGC 2 cut(s) 23, 618
FspBI CTAG 1 cut(s) 408
FspI TGCGCA 1 cut(s) 146
GlaI GCGC 3 cut(s) 66, 146, 790
GluI GCNGC 2 cut(s) 23, 618
GsaI CCCAGC 1 cut(s) 624
HaeII RGCGCY 2 cut(s) 68, 792
HaeIII GGCC 1 cut(s) 617
HapII CCGG 1 cut(s) 263
HhaI GCGC 3 cut(s) 67, 147, 791
Hin1I GRCGYC 1 cut(s) 789
Hin1II CATG 7 cut(s) 145, 529, 549, 677, 754, 760, 810
Hin6I GCGC 3 cut(s) 65, 145, 789
HinP1I GCGC 3 cut(s) 65, 145, 789
HinfI GANTC 2 cut(s) 541, 647
HpaII CCGG 1 cut(s) 263
HphI GGTGA 1 cut(s) 278
Hpy166II GTNNAC 2 cut(s) 628, 698
Hpy188I TCNGA 1 cut(s) 35
Hpy188III TCNNGA 3 cut(s) 600, 757, 807
Hpy8I GTNNAC 2 cut(s) 628, 698
Hpy99I CGWCG 1 cut(s) 662
HpyAV CCTTC 1 cut(s) 641
HpyCH4IV ACGT 1 cut(s) 503
HpyCH4V TGCA 6 cut(s) 29, 235, 389, 461, 491, 677
HpyF10VI GCNNNNNNNGC 5 cut(s) 232, 241, 302, 311, 560
HpyF3I CTNAG 3 cut(s) 18, 482, 743
HpySE526I ACGT 1 cut(s) 503
Hsp92I GRCGYC 1 cut(s) 789
Hsp92II CATG 7 cut(s) 145, 529, 549, 677, 754, 760, 810
HspAI GCGC 3 cut(s) 65, 145, 789
KasI GGCGCC 1 cut(s) 788
Ksp22I TGATCA 1 cut(s) 180
Kzo9I GATC 2 cut(s) 180, 438
Lsp1109I GCAGC 1 cut(s) 34
MaeI CTAG 1 cut(s) 408
MaeII ACGT 1 cut(s) 503
MaeIII GTNAC 2 cut(s) 94, 358
MalI GATC 2 cut(s) 182, 440
MbiI CCGCTC 1 cut(s) 436
MboI GATC 2 cut(s) 180, 438
MboII GAAGA 1 cut(s) 52
MhlI GDGCHC 3 cut(s) 165, 228, 286
MluCI AATT 4 cut(s) 148, 187, 555, 641
Mly113I GGCGCC 1 cut(s) 789
MnlI CCTC 4 cut(s) 29, 53, 262, 406
MseI TTAA 4 cut(s) 86, 249, 771, 795
MslI CAYNNNNRTG 1 cut(s) 755
MspA1I CMGCKG 1 cut(s) 620
MspI CCGG 1 cut(s) 263
MspR9I CCNGG 1 cut(s) 264
MwoI GCNNNNNNNGC 5 cut(s) 232, 241, 302, 311, 560
NarI GGCGCC 1 cut(s) 789
NciI CCSGG 1 cut(s) 264
NdeI CATATG 1 cut(s) 493
NdeII GATC 2 cut(s) 180, 438
NlaIII CATG 7 cut(s) 145, 529, 549, 677, 754, 760, 810
NlaIV GGNNCC 2 cut(s) 732, 790
NmuCI GTSAC 1 cut(s) 94
NsbI TGCGCA 1 cut(s) 146
NspI RCATGY 1 cut(s) 145
PagI TCATGA 2 cut(s) 756, 806
PfeI GAWTC 2 cut(s) 541, 647
PkrI GCNGC 2 cut(s) 24, 619
PluTI GGCGCC 1 cut(s) 792
PspFI CCCAGC 1 cut(s) 620
PspN4I GGNNCC 2 cut(s) 732, 790
PspPI GGNCC 2 cut(s) 615, 730
RsaI GTAC 1 cut(s) 169
RsaNI GTAC 1 cut(s) 168
RseI CAYNNNNRTG 1 cut(s) 755
SaqAI TTAA 4 cut(s) 86, 249, 771, 795
SatI GCNGC 2 cut(s) 23, 618
Sau3AI GATC 2 cut(s) 180, 438
Sau96I GGNCC 2 cut(s) 615, 730
ScrFI CCNGG 1 cut(s) 264
SduI GDGCHC 3 cut(s) 165, 228, 286
SetI ASST 9 cut(s) 27, 173, 273, 374, 452, 506, 521, 586, 633
SfoI GGCGCC 1 cut(s) 790
SinI GGWCC 1 cut(s) 730
SmiMI CAYNNNNRTG 1 cut(s) 755
SmlI CTYRAG 1 cut(s) 598
SmoI CTYRAG 1 cut(s) 598
Sse9I AATT 4 cut(s) 148, 187, 555, 641
SsiI CCGC 3 cut(s) 49, 436, 618
SspDI GGCGCC 1 cut(s) 788
SspMI CTAG 1 cut(s) 408
StyD4I CCNGG 1 cut(s) 262
TaiI ACGT 1 cut(s) 506
TasI AATT 4 cut(s) 148, 187, 555, 641
TauI GCSGC 1 cut(s) 620
TfiI GAWTC 2 cut(s) 541, 647
Tru1I TTAA 4 cut(s) 86, 249, 771, 795
Tru9I TTAA 4 cut(s) 86, 249, 771, 795
TscAI CASTG 1 cut(s) 700
TseFI GTSAC 1 cut(s) 94
TseI GCWGC 1 cut(s) 22
Tsp45I GTSAC 1 cut(s) 94
TspDTI ATGAA 4 cut(s) 460, 676, 773, 795
TspGWI ACGGA 1 cut(s) 640
TspRI CASTG 1 cut(s) 700
VpaK11BI GGWCC 1 cut(s) 730
XapI RAATTY 2 cut(s) 187, 641
XceI RCATGY 1 cut(s) 145
XspI CTAG 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.