Rroxscaffold_7G00158110

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0002925
Physical Location & Seq
Reverse (-)
1667652 .. 1670535
2884 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00158110.1

Sequence Viewer

Length: 669 bp
ATGGTGTGGATGGTAACTCAACAGTTTGTGATTAAAGGAAAGAGAAGGCGCATATGGGGAGGAGCTTTTCTCTGTTGGGTGCTGTTAATGTTGGTCACTCCCAAAATTTCTCACTCCCCAAATCACCATCACTACGTTGACATGCGCAATTTCCTTGGAGTGCCCAACACATTGAATGTGATCACCAGTTTCCCATTCTTGGTTGTTGGGGTTCTGGGTTTTGTTCTCTGTGTCCAAGGAGGCTTTTTCAATATCAGTTTGCCAGGTGAAGTTTGGGGTTGGGCACTGTTCTATGCAGGAATAGCAGGGCTGGCTTTTGGGTCTGCTTATTATCATTTGAAGCCTGATGATAGTAGAGTGACTTGGGATACCTTGCCGATGATGATTGCATTCTCCTCGCTTTTCTCTAGTTTTATAGTGGAGAGAGTGGGAGAGAGAATTGGCTTAAGTTGTCTATTTGCACTCCTCGTTATTGCGGTTCTTAGTACTGCTTATGAGAGAACATGCAATGATATCAGATTGTACATGATGTTCCAATTGATTCCATGTATAGCAATTCCAGGAATGACATTTGTGTTCCCACCCAAATATACTCACTCAAGATATTGGCTTTGGGCAGCAGTGACTACAGCAACTCTATACAGGGGCTTACATTCTCTCCAAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ceramidase

Protein Analysis

222

Amino Acids

25.2

Weight (kDa)

9.35

Isoelectric Point (pI)

31.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ceramidase PF05875 57 - 181 6.3e-07 Ceramidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 146
AciI CCGC 1 cut(s) 476
AcsI RAATTY 2 cut(s) 105, 663
AfaI GTAC 2 cut(s) 487, 524
AfiI CCNNNNNNNGG 1 cut(s) 199
AflII CTTAAG 1 cut(s) 445
AgsI TTSAA 3 cut(s) 175, 250, 340
AjnI CCWGG 2 cut(s) 262, 559
AluBI AGCT 1 cut(s) 65
AluI AGCT 1 cut(s) 65
ApeKI GCWGC 1 cut(s) 617
ApoI RAATTY 2 cut(s) 105, 663
AspLEI GCGC 2 cut(s) 51, 147
AsuHPI GGTGA 3 cut(s) 116, 175, 278
BaeGI GKGCMC 2 cut(s) 165, 286
BbvI GCAGC 1 cut(s) 629
BccI CCATC 2 cut(s) 4, 135
BcgI CGANNNNNNTGC 2 cut(s) 378, 412
BciT130I CCWGG 2 cut(s) 264, 561
BciVI GTATCC 1 cut(s) 361
BclI TGATCA 1 cut(s) 180
BfaI CTAG 1 cut(s) 408
BfmI CTRYAG 1 cut(s) 627
BfrI CTTAAG 1 cut(s) 445
BfuI GTATCC 1 cut(s) 361
BisI GCNGC 1 cut(s) 618
BlsI GCNGC 1 cut(s) 619
BmcAI AGTACT 1 cut(s) 487
Bme1390I CCNGG 2 cut(s) 264, 561
BmrFI CCNGG 2 cut(s) 264, 561
BplI GAGNNNNNCTC 2 cut(s) 54, 86
BpuEI CTTGAG 1 cut(s) 583
BsaJI CCNNGG 2 cut(s) 154, 235
BsaXI ACNNNNNCTCC 1 cut(s) 642
Bsc4I CCNNNNNNNGG 1 cut(s) 199
Bse1I ACTGG 1 cut(s) 186
Bse3DI GCAATG 1 cut(s) 514
BseBI CCWGG 2 cut(s) 264, 561
BseDI CCNNGG 2 cut(s) 154, 235
BseGI GGATG 1 cut(s) 15
BseLI CCNNNNNNNGG 1 cut(s) 199
BseMI GCAATG 1 cut(s) 514
BseNI ACTGG 1 cut(s) 186
BseRI GAGGAG 3 cut(s) 75, 385, 455
BseSI GKGCMC 2 cut(s) 165, 286
BseXI GCAGC 1 cut(s) 629
BslI CCNNNNNNNGG 1 cut(s) 199
BsmI GAATGC 1 cut(s) 389
Bsp1286I GDGCHC 2 cut(s) 165, 286
Bsp1407I TGTACA 1 cut(s) 522
Bsp143I GATC 1 cut(s) 180
BspACI CCGC 1 cut(s) 476
BspTI CTTAAG 1 cut(s) 445
BsrDI GCAATG 1 cut(s) 514
BsrGI TGTACA 1 cut(s) 522
BsrI ACTGG 1 cut(s) 186
BssECI CCNNGG 2 cut(s) 154, 235
BssMI GATC 1 cut(s) 180
BssT1I CCWWGG 2 cut(s) 154, 235
Bst2UI CCWGG 2 cut(s) 264, 561
Bst4CI ACNGT 2 cut(s) 24, 288
BstAFI CTTAAG 1 cut(s) 445
BstAUI TGTACA 1 cut(s) 522
BstC8I GCNNGC 1 cut(s) 312
BstDEI CTNAG 1 cut(s) 482
BstF5I GGATG 1 cut(s) 15
BstHHI GCGC 2 cut(s) 51, 147
BstKTI GATC 1 cut(s) 183
BstMBI GATC 1 cut(s) 180
BstMWI GCNNNNNNNGC 2 cut(s) 302, 311
BstNI CCWGG 2 cut(s) 264, 561
BstNSI RCATGY 2 cut(s) 145, 507
BstSCI CCNGG 2 cut(s) 262, 559
BstSFI CTRYAG 1 cut(s) 627
BstSLI GKGCMC 2 cut(s) 165, 286
BstV1I GCAGC 1 cut(s) 629
BsuI GTATCC 1 cut(s) 361
BtsCI GGATG 1 cut(s) 15
BtsI GCAGTG 1 cut(s) 627
BtsIMutI CAGTG 2 cut(s) 284, 627
Cac8I GCNNGC 1 cut(s) 312
CfoI GCGC 2 cut(s) 51, 147
Csp6I GTAC 2 cut(s) 486, 523
CviAII CATG 4 cut(s) 142, 504, 526, 546
CviJI RGCY 8 cut(s) 65, 243, 310, 314, 343, 444, 610, 648
CviKI_1 RGCY 8 cut(s) 65, 243, 310, 314, 343, 444, 610, 648
CviQI GTAC 2 cut(s) 486, 523
DdeI CTNAG 1 cut(s) 482
DpnI GATC 1 cut(s) 182
DpnII GATC 1 cut(s) 180
Eco130I CCWWGG 2 cut(s) 154, 235
Eco32I GATATC 1 cut(s) 514
EcoRII CCWGG 2 cut(s) 262, 559
EcoRV GATATC 1 cut(s) 514
EcoT14I CCWWGG 2 cut(s) 154, 235
ErhI CCWWGG 2 cut(s) 154, 235
FaeI CATG 4 cut(s) 145, 507, 529, 549
FatI CATG 4 cut(s) 141, 503, 525, 545
FauNDI CATATG 1 cut(s) 53
FbaI TGATCA 1 cut(s) 180
Fnu4HI GCNGC 1 cut(s) 618
FokI GGATG 1 cut(s) 22
Fsp4HI GCNGC 1 cut(s) 618
FspBI CTAG 1 cut(s) 408
FspI TGCGCA 1 cut(s) 146
GlaI GCGC 2 cut(s) 50, 146
GluI GCNGC 1 cut(s) 618
HhaI GCGC 2 cut(s) 51, 147
Hin1II CATG 4 cut(s) 145, 507, 529, 549
Hin6I GCGC 2 cut(s) 49, 145
HinP1I GCGC 2 cut(s) 49, 145
HincII GTYRAC 1 cut(s) 139
HindII GTYRAC 1 cut(s) 139
HinfI GANTC 1 cut(s) 541
HphI GGTGA 3 cut(s) 116, 175, 278
Hpy166II GTNNAC 1 cut(s) 139
Hpy188I TCNGA 1 cut(s) 518
Hpy188III TCNNGA 1 cut(s) 600
Hpy8I GTNNAC 1 cut(s) 139
HpyAV CCTTC 1 cut(s) 39
HpyCH4III ACNGT 2 cut(s) 24, 288
HpyCH4IV ACGT 1 cut(s) 135
HpyCH4V TGCA 4 cut(s) 296, 389, 461, 507
HpyF10VI GCNNNNNNNGC 2 cut(s) 302, 311
HpyF3I CTNAG 1 cut(s) 482
HpySE526I ACGT 1 cut(s) 135
Hsp92II CATG 4 cut(s) 145, 507, 529, 549
HspAI GCGC 2 cut(s) 49, 145
Ksp22I TGATCA 1 cut(s) 180
Kzo9I GATC 1 cut(s) 180
LmnI GCTCC 1 cut(s) 62
Lsp1109I GCAGC 1 cut(s) 629
MaeI CTAG 1 cut(s) 408
MaeII ACGT 1 cut(s) 135
MaeIII GTNAC 4 cut(s) 13, 94, 358, 622
MalI GATC 1 cut(s) 182
MboI GATC 1 cut(s) 180
MfeI CAATTG 1 cut(s) 536
MhlI GDGCHC 2 cut(s) 165, 286
MluCI AATT 6 cut(s) 105, 148, 438, 536, 555, 663
MnlI CCTC 4 cut(s) 53, 233, 406, 476
MseI TTAA 3 cut(s) 33, 86, 446
MspCI CTTAAG 1 cut(s) 445
MspR9I CCNGG 2 cut(s) 264, 561
MunI CAATTG 1 cut(s) 536
Mva1269I GAATGC 1 cut(s) 389
MvaI CCWGG 2 cut(s) 264, 561
MwoI GCNNNNNNNGC 2 cut(s) 302, 311
NdeI CATATG 1 cut(s) 53
NdeII GATC 1 cut(s) 180
NlaIII CATG 4 cut(s) 145, 507, 529, 549
NmuCI GTSAC 3 cut(s) 94, 358, 622
NsbI TGCGCA 1 cut(s) 146
NspI RCATGY 2 cut(s) 145, 507
PctI GAATGC 1 cut(s) 389
PfeI GAWTC 1 cut(s) 541
PfoI TCCNGGA 1 cut(s) 559
PkrI GCNGC 1 cut(s) 619
Psp6I CCWGG 2 cut(s) 262, 559
PspGI CCWGG 2 cut(s) 262, 559
PsrI GAACNNNNNNTAC 2 cut(s) 515, 547
RsaI GTAC 2 cut(s) 487, 524
RsaNI GTAC 2 cut(s) 486, 523
SaqAI TTAA 3 cut(s) 33, 86, 446
SatI GCNGC 1 cut(s) 618
Sau3AI GATC 1 cut(s) 180
ScaI AGTACT 1 cut(s) 487
ScrFI CCNGG 2 cut(s) 264, 561
SduI GDGCHC 2 cut(s) 165, 286
SetI ASST 4 cut(s) 67, 138, 268, 374
SfcI CTRYAG 1 cut(s) 627
SmlI CTYRAG 2 cut(s) 445, 598
SmoI CTYRAG 2 cut(s) 445, 598
Sse9I AATT 6 cut(s) 105, 148, 438, 536, 555, 663
SsiI CCGC 1 cut(s) 476
SspMI CTAG 1 cut(s) 408
StyD4I CCNGG 2 cut(s) 262, 559
StyI CCWWGG 2 cut(s) 154, 235
TaaI ACNGT 2 cut(s) 24, 288
TaiI ACGT 1 cut(s) 138
TasI AATT 6 cut(s) 105, 148, 438, 536, 555, 663
TatI WGTACW 2 cut(s) 485, 522
TfiI GAWTC 1 cut(s) 541
Tru1I TTAA 3 cut(s) 33, 86, 446
Tru9I TTAA 3 cut(s) 33, 86, 446
TscAI CASTG 2 cut(s) 291, 627
TseFI GTSAC 3 cut(s) 94, 358, 622
TseI GCWGC 1 cut(s) 617
Tsp45I GTSAC 3 cut(s) 94, 358, 622
TspRI CASTG 2 cut(s) 291, 627
Vha464I CTTAAG 1 cut(s) 445
XapI RAATTY 2 cut(s) 105, 663
XceI RCATGY 2 cut(s) 145, 507
XcmI CCANNNNNNNNNTGG 1 cut(s) 270
XspI CTAG 1 cut(s) 408
ZrmI AGTACT 1 cut(s) 487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.