FvH4_2g32200

Protein of unknown function (DUF1639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0015556
Physical Location & Seq
Forward (+)
24495206 .. 24500946
5741 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g32200.t1

Sequence Viewer

Length: 861 bp
ATGGAAAGAGACTGTGTGAGGCACCCCAATATTAACGACTCCAAGAATTCTAGAGACAGAAGCGGAGGGGATAGCTCACTGCTGTTGAGATCCAGTCCTGATAAAACGACGACGTCCGAGTTCGTTTTGCAGTGGGGTAATCGCAAGCGTCTCCGGTGCATGAAGATCCAGGTCAAGGACGACTCCAGCGGTCTCCCGGTTACCCGAACCACTGTTCGGGTCGACCGCCGGGTCATTCGATCCGACAAAGACTCGTCTAATCATCCCTCTAATAGTAACGGGTATTTGAACCTGCGTCAGAGGCCGTTGTCGCCGCAACAACCGCCGCTTCAGCGCGTTCTCAGAAACTCTGAGACTTCAAGTGCATCAGCCATGAGAGCCCATCAGAGCAACGGCGGAGCTGCCAGGGGGATTGTATCGCCTGACAGGGGCGGTGGTGCGCACGATAAGAAAGGTACAGCTAACAACCACAACCATCACAATCACCATAATGACAATCATCACAACAACCACAAGTCCGCCGCGTCTTCCGAGACGGCGCACGATAGCAAGAAAGGCGGCTCGTCTTCCGGGAGCGGCGACGCGGCTGCACCGCCCATGGTGTGGCCGCCCAAGTTCGTGATTGCCTTGACCAACAAAGAAAAGGAAGAGGATTTCATGGCCATTAAGGGATCCAAGCTCCCTCAGCGACCCAAAAAGAGAGCAAAGTTCATCCAACGCACCCTCAATCTGGTAAGCCCGGGCGCATGGCTGTGTGATCTGACCCTAGAACGGTACGAGGTCAGAGAGAAGAAGATTTCCAAGAAGAGACCGAGAGGATTGAAGGCGATGGGCACTATGGTGGAGTCGGACTCGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1639

Protein Analysis

287

Amino Acids

31.73

Weight (kDa)

10.25

Isoelectric Point (pI)

56.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1639 PF07797 210 - 259 9.9e-23 Protein of unknown function (DUF1639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015556)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g32200
malus_domestica MD08G1073700.v1.1
prunus_persica Prupe.1G415100_v2.0.a1
pyrus_communis pycom08g06100 pycom15g05800
rosa_chinensis RchiOBHm_Chr6g0310471
rosa_laevigata RLG00000010480 RLG00000018734
rosa_multiflora Rmu_sc0020729.1_g000006
rosa_rugosa Rorug06G0380600
rosa_samantha Rh4DG040600 Rh6AG494600 Rh6BG504300 Rh6CG509500 Rh6DG495500
rosa_wichuraiana Rw6G043020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 230
AatII GACGTC 1 cut(s) 116
Acc16I TGCGCA 1 cut(s) 441
Acc36I ACCTGC 1 cut(s) 300
AccB1I GGYRCC 1 cut(s) 21
AccB7I CCANNNNNTGG 1 cut(s) 603
AccBSI CCGCTC 1 cut(s) 576
AccI GTMKAC 1 cut(s) 222
AccII CGCG 3 cut(s) 336, 524, 584
AclWI GGATC 5 cut(s) 84, 160, 234, 666, 679
AcoI YGGCCR 2 cut(s) 605, 660
AcsI RAATTY 1 cut(s) 46
AcuI CTGAAG 1 cut(s) 314
AcyI GRCGYC 1 cut(s) 113
AfaI GTAC 2 cut(s) 457, 776
AfiI CCNNNNNNNGG 6 cut(s) 175, 216, 428, 603, 730, 771
AgsI TTSAA 3 cut(s) 289, 360, 823
AjnI CCWGG 2 cut(s) 168, 404
AleI CACNNNNGTG 1 cut(s) 839
AluBI AGCT 4 cut(s) 75, 401, 461, 679
AluI AGCT 4 cut(s) 75, 401, 461, 679
Alw26I GTCTC 7 cut(s) 3, 48, 155, 197, 347, 527, 802
AlwI GGATC 5 cut(s) 84, 160, 234, 666, 679
Ama87I CYCGRG 1 cut(s) 739
AoxI GGCC 3 cut(s) 302, 605, 660
ApeKI GCWGC 2 cut(s) 401, 587
ApoI RAATTY 1 cut(s) 46
AspLEI GCGC 4 cut(s) 336, 442, 541, 746
AsuC2I CCSGG 5 cut(s) 197, 230, 571, 740, 741
AsuHPI GGTGA 1 cut(s) 476
AvaI CYCGRG 1 cut(s) 739
BaeGI GKGCMC 1 cut(s) 836
BalI TGGCCA 1 cut(s) 662
BamHI GGATCC 1 cut(s) 671
BanI GGYRCC 1 cut(s) 21
BanII GRGCYC 1 cut(s) 382
BbsI GAAGAC 2 cut(s) 519, 558
BbvCI CCTCAGC 1 cut(s) 684
BbvI GCAGC 2 cut(s) 388, 574
BccI CCATC 3 cut(s) 390, 483, 823
BceAI ACGGC 3 cut(s) 289, 409, 552
BcgI CGANNNNNNTGC 2 cut(s) 569, 603
BciT130I CCWGG 2 cut(s) 170, 406
BcnI CCSGG 5 cut(s) 197, 230, 571, 740, 741
BcoDI GTCTC 7 cut(s) 3, 48, 155, 197, 347, 527, 802
BfaI CTAG 2 cut(s) 51, 767
BfuAI ACCTGC 1 cut(s) 300
BisI GCNGC 9 cut(s) 314, 326, 402, 522, 559, 577, 585, 588, 608
BlsI GCNGC 9 cut(s) 315, 327, 403, 523, 560, 578, 586, 589, 609
Bme1390I CCNGG 7 cut(s) 170, 197, 230, 406, 571, 740, 741
BmeT110I CYCGRG 1 cut(s) 739
BmiI GGNNCC 2 cut(s) 23, 673
BmrFI CCNGG 7 cut(s) 170, 197, 230, 406, 571, 740, 741
BmsI GCATC 1 cut(s) 374
BpiI GAAGAC 2 cut(s) 519, 558
BplI GAGNNNNNCTC 1 cut(s) 836
BpmI CTGGAG 1 cut(s) 169
Bpu10I CCTNAGC 1 cut(s) 684
BpuMI CCSGG 5 cut(s) 197, 230, 571, 740, 741
BsaHI GRCGYC 1 cut(s) 113
BsaI GGTCTC 2 cut(s) 197, 802
BsaJI CCNNGG 3 cut(s) 405, 597, 739
BsaWI WCCGGW 1 cut(s) 153
Bsc4I CCNNNNNNNGG 6 cut(s) 175, 216, 428, 603, 730, 771
Bse1I ACTGG 1 cut(s) 93
BseBI CCWGG 2 cut(s) 170, 406
BseDI CCNNGG 3 cut(s) 405, 597, 739
BseGI GGATG 2 cut(s) 262, 711
BseLI CCNNNNNNNGG 6 cut(s) 175, 216, 428, 603, 730, 771
BseMII CTCAG 3 cut(s) 342, 355, 698
BseNI ACTGG 1 cut(s) 93
BseSI GKGCMC 1 cut(s) 836
BseXI GCAGC 2 cut(s) 388, 574
BsgI GTGCAG 1 cut(s) 573
Bsh1236I CGCG 3 cut(s) 336, 524, 584
Bsh1285I CGRYCG 1 cut(s) 226
BshFI GGCC 3 cut(s) 304, 607, 662
BshNI GGYRCC 1 cut(s) 21
BsiEI CGRYCG 1 cut(s) 226
BsiHKCI CYCGRG 1 cut(s) 739
BsiSI CCGG 5 cut(s) 154, 197, 229, 570, 740
BslI CCNNNNNNNGG 6 cut(s) 175, 216, 428, 603, 730, 771
BsmAI GTCTC 7 cut(s) 3, 48, 155, 197, 347, 527, 802
BsmBI CGTCTC 2 cut(s) 155, 527
BsnI GGCC 3 cut(s) 304, 607, 662
Bso31I GGTCTC 2 cut(s) 197, 802
BsoBI CYCGRG 1 cut(s) 739
Bsp1286I GDGCHC 2 cut(s) 382, 836
Bsp143I GATC 5 cut(s) 89, 165, 239, 671, 757
Bsp19I CCATGG 1 cut(s) 597
BspANI GGCC 3 cut(s) 304, 607, 662
BspCNI CTCAG 3 cut(s) 343, 354, 697
BspFNI CGCG 3 cut(s) 336, 524, 584
BspLI GGNNCC 2 cut(s) 23, 673
BspMI ACCTGC 1 cut(s) 300
BspPI GGATC 5 cut(s) 84, 160, 234, 666, 679
BspT107I GGYRCC 1 cut(s) 21
BspTNI GGTCTC 2 cut(s) 197, 802
BsrBI CCGCTC 1 cut(s) 576
BsrI ACTGG 1 cut(s) 93
BssECI CCNNGG 3 cut(s) 405, 597, 739
BssMI GATC 5 cut(s) 89, 165, 239, 671, 757
BssNI GRCGYC 1 cut(s) 113
BssT1I CCWWGG 1 cut(s) 597
Bst2UI CCWGG 2 cut(s) 170, 406
Bst4CI ACNGT 3 cut(s) 14, 214, 774
Bst6I CTCTTC 2 cut(s) 642, 800
BstACI GRCGYC 1 cut(s) 113
BstC8I GCNNGC 1 cut(s) 146
BstDEI CTNAG 3 cut(s) 341, 351, 684
BstDSI CCRYGG 1 cut(s) 597
BstEII GGTNACC 1 cut(s) 199
BstF5I GGATG 2 cut(s) 262, 711
BstFNI CGCG 3 cut(s) 336, 524, 584
BstHHI GCGC 4 cut(s) 336, 442, 541, 746
BstKTI GATC 5 cut(s) 92, 168, 242, 674, 760
BstMAI GTCTC 7 cut(s) 3, 48, 155, 197, 347, 527, 802
BstMBI GATC 5 cut(s) 89, 165, 239, 671, 757
BstMCI CGRYCG 1 cut(s) 226
BstMWI GCNNNNNNNGC 7 cut(s) 301, 310, 322, 331, 377, 555, 685
BstNI CCWGG 2 cut(s) 170, 406
BstPI GGTNACC 1 cut(s) 199
BstSCI CCNGG 7 cut(s) 168, 195, 228, 404, 569, 738, 739
BstSLI GKGCMC 1 cut(s) 836
BstUI CGCG 3 cut(s) 336, 524, 584
BstV1I GCAGC 2 cut(s) 388, 574
BstV2I GAAGAC 2 cut(s) 519, 558
BstX2I RGATCY 3 cut(s) 89, 165, 671
BstYI RGATCY 3 cut(s) 89, 165, 671
BsuRI GGCC 3 cut(s) 304, 607, 662
BtgI CCRYGG 1 cut(s) 597
BtgZI GCGATG 1 cut(s) 842
BtsCI GGATG 2 cut(s) 262, 711
BtsI GCAGTG 2 cut(s) 77, 137
BtsIMutI CAGTG 3 cut(s) 77, 137, 210
BveI ACCTGC 1 cut(s) 300
Cac8I GCNNGC 1 cut(s) 146
CfoI GCGC 4 cut(s) 336, 442, 541, 746
Cfr9I CCCGGG 1 cut(s) 739
CseI GACGC 4 cut(s) 137, 284, 513, 590
Csp6I GTAC 2 cut(s) 456, 775
CviAII CATG 5 cut(s) 160, 373, 598, 658, 747
CviQI GTAC 2 cut(s) 456, 775
DdeI CTNAG 3 cut(s) 341, 351, 684
DpnI GATC 5 cut(s) 91, 167, 241, 673, 759
DpnII GATC 5 cut(s) 89, 165, 239, 671, 757
DrdI GACNNNNNNGTC 1 cut(s) 230
DseDI GACNNNNNNGTC 1 cut(s) 230
EaeI YGGCCR 2 cut(s) 605, 660
Eam1104I CTCTTC 2 cut(s) 642, 800
EarI CTCTTC 2 cut(s) 642, 800
EciI GGCGGA 2 cut(s) 411, 508
Eco130I CCWWGG 1 cut(s) 597
Eco24I GRGCYC 1 cut(s) 382
Eco31I GGTCTC 2 cut(s) 197, 802
Eco57I CTGAAG 1 cut(s) 314
Eco88I CYCGRG 1 cut(s) 739
Eco91I GGTNACC 1 cut(s) 199
EcoO65I GGTNACC 1 cut(s) 199
EcoRI GAATTC 1 cut(s) 46
EcoRII CCWGG 2 cut(s) 168, 404
EcoT14I CCWWGG 1 cut(s) 597
EcoT38I GRGCYC 1 cut(s) 382
ErhI CCWWGG 1 cut(s) 597
Esp3I CGTCTC 2 cut(s) 155, 527
FaeI CATG 5 cut(s) 163, 376, 601, 661, 750
FaiI YATR 7 cut(s) 161, 374, 489, 599, 659, 748, 839
FatI CATG 5 cut(s) 159, 372, 597, 657, 746
FblI GTMKAC 1 cut(s) 222
Fnu4HI GCNGC 9 cut(s) 314, 326, 402, 522, 559, 577, 585, 588, 608
FokI GGATG 2 cut(s) 249, 698
FriOI GRGCYC 1 cut(s) 382
Fsp4HI GCNGC 9 cut(s) 314, 326, 402, 522, 559, 577, 585, 588, 608
FspAI RTGCGCAY 1 cut(s) 441
FspBI CTAG 2 cut(s) 51, 767
FspI TGCGCA 1 cut(s) 441
GlaI GCGC 4 cut(s) 335, 441, 540, 745
GluI GCNGC 9 cut(s) 314, 326, 402, 522, 559, 577, 585, 588, 608
GsuI CTGGAG 1 cut(s) 169
HaeIII GGCC 3 cut(s) 304, 607, 662
HapII CCGG 5 cut(s) 154, 197, 229, 570, 740
HgaI GACGC 4 cut(s) 137, 284, 513, 590
HhaI GCGC 4 cut(s) 336, 442, 541, 746
Hin1I GRCGYC 1 cut(s) 113
Hin1II CATG 5 cut(s) 163, 376, 601, 661, 750
Hin6I GCGC 4 cut(s) 334, 440, 539, 744
HinP1I GCGC 4 cut(s) 334, 440, 539, 744
HincII GTYRAC 1 cut(s) 223
HindII GTYRAC 1 cut(s) 223
HinfI GANTC 5 cut(s) 38, 182, 251, 845, 851
HpaII CCGG 5 cut(s) 154, 197, 229, 570, 740
HphI GGTGA 1 cut(s) 476
Hpy166II GTNNAC 1 cut(s) 223
Hpy188III TCNNGA 3 cut(s) 51, 98, 619
Hpy8I GTNNAC 1 cut(s) 223
Hpy99I CGWCG 3 cut(s) 112, 115, 584
HpyAV CCTTC 1 cut(s) 817
HpyCH4III ACNGT 3 cut(s) 14, 214, 774
HpyCH4IV ACGT 1 cut(s) 113
HpyCH4V TGCA 4 cut(s) 130, 159, 365, 590
HpyF10VI GCNNNNNNNGC 7 cut(s) 301, 310, 322, 331, 377, 555, 685
HpyF3I CTNAG 3 cut(s) 341, 351, 684
HpySE526I ACGT 1 cut(s) 113
Hsp92I GRCGYC 1 cut(s) 113
Hsp92II CATG 5 cut(s) 163, 376, 601, 661, 750
HspAI GCGC 4 cut(s) 334, 440, 539, 744
Kzo9I GATC 5 cut(s) 89, 165, 239, 671, 757
LmnI GCTCC 3 cut(s) 398, 573, 684
Lsp1109I GCAGC 2 cut(s) 388, 574
LweI GCATC 1 cut(s) 374
MaeI CTAG 2 cut(s) 51, 767
MaeII ACGT 1 cut(s) 113
MaeIII GTNAC 2 cut(s) 199, 275
MalI GATC 5 cut(s) 91, 167, 241, 673, 759
MbiI CCGCTC 1 cut(s) 576
MboI GATC 5 cut(s) 89, 165, 239, 671, 757
MboII GAAGA 7 cut(s) 175, 519, 558, 659, 802, 805, 817
MflI RGATCY 3 cut(s) 89, 165, 671
MhlI GDGCHC 2 cut(s) 382, 836
MlsI TGGCCA 1 cut(s) 662
MluCI AATT 1 cut(s) 46
MluNI TGGCCA 1 cut(s) 662
MlyI GAGTC 5 cut(s) 32, 176, 245, 845, 854
MmeI TCCRAC 3 cut(s) 267, 739, 828
MnlI CCTC 9 cut(s) 12, 59, 277, 294, 643, 693, 734, 772, 809
Mox20I TGGCCA 1 cut(s) 662
MscI TGGCCA 1 cut(s) 662
MseI TTAA 2 cut(s) 33, 666
MslI CAYNNNNRTG 3 cut(s) 489, 751, 839
Msp20I TGGCCA 1 cut(s) 662
MspA1I CMGCKG 1 cut(s) 189
MspI CCGG 5 cut(s) 154, 197, 229, 570, 740
MspR9I CCNGG 7 cut(s) 170, 197, 230, 406, 571, 740, 741
MvaI CCWGG 2 cut(s) 170, 406
MvnI CGCG 3 cut(s) 336, 524, 584
MwoI GCNNNNNNNGC 7 cut(s) 301, 310, 322, 331, 377, 555, 685
NciI CCSGG 5 cut(s) 197, 230, 571, 740, 741
NcoI CCATGG 1 cut(s) 597
NdeII GATC 5 cut(s) 89, 165, 239, 671, 757
NlaIII CATG 5 cut(s) 163, 376, 601, 661, 750
NlaIV GGNNCC 2 cut(s) 23, 673
NsbI TGCGCA 1 cut(s) 441
OliI CACNNNNGTG 1 cut(s) 839
PflFI GACNNNGTC 1 cut(s) 112
PflMI CCANNNNNTGG 1 cut(s) 603
PfoI TCCNGGA 1 cut(s) 569
PkrI GCNGC 9 cut(s) 315, 327, 403, 523, 560, 578, 586, 589, 609
PleI GAGTC 5 cut(s) 32, 176, 245, 845, 853
PpsI GAGTC 5 cut(s) 32, 176, 245, 845, 853
Psp6I CCWGG 2 cut(s) 168, 404
PspEI GGTNACC 1 cut(s) 199
PspGI CCWGG 2 cut(s) 168, 404
PspN4I GGNNCC 2 cut(s) 23, 673
PsuI RGATCY 3 cut(s) 89, 165, 671
PsyI GACNNNGTC 1 cut(s) 112
RsaI GTAC 2 cut(s) 457, 776
RsaNI GTAC 2 cut(s) 456, 775
RseI CAYNNNNRTG 3 cut(s) 489, 751, 839
SalI GTCGAC 1 cut(s) 221
SaqAI TTAA 2 cut(s) 33, 666
SatI GCNGC 9 cut(s) 314, 326, 402, 522, 559, 577, 585, 588, 608
Sau3AI GATC 5 cut(s) 89, 165, 239, 671, 757
SchI GAGTC 5 cut(s) 32, 176, 245, 845, 854
ScrFI CCNGG 7 cut(s) 170, 197, 230, 406, 571, 740, 741
SduI GDGCHC 2 cut(s) 382, 836
SetI ASST 9 cut(s) 77, 116, 174, 294, 403, 457, 463, 681, 783
SfaNI GCATC 1 cut(s) 374
SmaI CCCGGG 1 cut(s) 741
SmiMI CAYNNNNRTG 3 cut(s) 489, 751, 839
SrfI GCCCGGGC 1 cut(s) 741
Sse9I AATT 1 cut(s) 46
SspI AATATT 1 cut(s) 31
SspMI CTAG 2 cut(s) 51, 767
StyD4I CCNGG 7 cut(s) 168, 195, 228, 404, 569, 738, 739
StyI CCWWGG 1 cut(s) 597
TaaI ACNGT 3 cut(s) 14, 214, 774
TaiI ACGT 1 cut(s) 116
TaqI TCGA 2 cut(s) 222, 238
TaqII GACCGA 1 cut(s) 826
TasI AATT 1 cut(s) 46
TauI GCSGC 7 cut(s) 316, 328, 524, 561, 579, 587, 610
Tru1I TTAA 2 cut(s) 33, 666
Tru9I TTAA 2 cut(s) 33, 666
TscAI CASTG 3 cut(s) 84, 137, 217
TseI GCWGC 2 cut(s) 401, 587
TspDTI ATGAA 3 cut(s) 176, 646, 700
TspMI CCCGGG 1 cut(s) 739
TspRI CASTG 3 cut(s) 84, 137, 217
Tth111I GACNNNGTC 1 cut(s) 112
Van91I CCANNNNNTGG 1 cut(s) 603
XapI RAATTY 1 cut(s) 46
XbaI TCTAGA 1 cut(s) 50
XmaI CCCGGG 1 cut(s) 739
XmiI GTMKAC 1 cut(s) 222
XspI CTAG 2 cut(s) 51, 767
ZraI GACGTC 1 cut(s) 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.