MD08G1073700.v1.1

Protein of unknown function (DUF1639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0015556
Physical Location & Seq
Forward (+)
5943326 .. 5950581
7256 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1073700.v1.1.491

Sequence Viewer

Length: 711 bp
ATGAAGATCCAGGTGAAGGAGAAGGACGACTCCAGCGGCCCCCCGGTTACCCGAACCACGGTCCGCGTCAGTCGCCGGGTTGTTCGACCCGATAAGGACCCGGCGAATCAGCTTTCCACCACCGTTAATCAGACGAGTAACGGCTATTTGAATATCCGTCAACGGCCGTTATCTCCCCAGCAACCGCCACCTCAGCGTATTCTCAGAAACTCAGAGACTACGAGCGCTGCTGCCATGAGAGGTCAGAGCAACGGCGGAGGAGGAGGGCTGAGGGGAATTGCCTCGCCCGACAGGGGTGCACACGATAAGAAAAACCACCACCGCCACCACCATAATGATGAGATTAATCATCACAATAACAAGTCCGCGGCGTCTTCGGACACGGCGCACGATAGCAAGAAGGGCGGGTCTTCGTCGGGGAGCGGAGAGGCGGCGGTGGGGCCAGAGATGGTGTGGCCGCCGAAGTTCGTGATTGCTTTGACCAATAAGGAGAAAGAAGAGGACTTTATGGCAATCAAAGGGTCGAAATTGCCTCAGAGACCAAAGAAGCGAGCCAAGTTCGTTCAACGCACCCTCAATCTTGTAAGCCCGGGAGCTTGGCTCTGTGATTTGACGCTGGAACGGTACGAGGTCCGGGAGAAGAAGATTTCGAAAAAGAGACCAAGAGGGTTAAAGGCAATGGGCAATGTGGTGGAGTCGGACTCTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1639

Protein Analysis

237

Amino Acids

25.98

Weight (kDa)

10.2

Isoelectric Point (pI)

48.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1639 PF07797 160 - 209 3.1e-23 Protein of unknown function (DUF1639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015556)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g32200
malus_domestica MD08G1073700.v1.1
prunus_persica Prupe.1G415100_v2.0.a1
pyrus_communis pycom08g06100 pycom15g05800
rosa_chinensis RchiOBHm_Chr6g0310471
rosa_laevigata RLG00000010480 RLG00000018734
rosa_multiflora Rmu_sc0020729.1_g000006
rosa_rugosa Rorug06G0380600
rosa_samantha Rh4DG040600 Rh6AG494600 Rh6BG504300 Rh6CG509500 Rh6DG495500
rosa_wichuraiana Rw6G043020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 423
AccII CGCG 2 cut(s) 66, 368
AcoI YGGCCR 2 cut(s) 164, 455
AcyI GRCGYC 1 cut(s) 371
AfaI GTAC 1 cut(s) 626
AfeI AGCGCT 1 cut(s) 226
AfiI CCNNNNNNNGG 3 cut(s) 16, 58, 293
AgsI TTSAA 2 cut(s) 151, 566
AjnI CCWGG 1 cut(s) 9
AluBI AGCT 2 cut(s) 112, 596
AluI AGCT 2 cut(s) 112, 596
Alw21I GWGCWC 1 cut(s) 301
Alw26I GTCTC 3 cut(s) 209, 532, 652
Alw44I GTGCAC 1 cut(s) 297
Ama87I CYCGRG 1 cut(s) 589
Aor51HI AGCGCT 1 cut(s) 226
AoxI GGCC 4 cut(s) 37, 164, 440, 455
ApaLI GTGCAC 1 cut(s) 297
ApeKI GCWGC 2 cut(s) 227, 230
AseI ATTAAT 1 cut(s) 345
AspLEI GCGC 2 cut(s) 227, 388
AspS9I GGNCC 5 cut(s) 38, 61, 97, 440, 631
AsuC2I CCSGG 6 cut(s) 44, 77, 101, 590, 591, 635
AsuHPI GGTGA 1 cut(s) 25
AsuII TTCGAA 1 cut(s) 650
AvaI CYCGRG 1 cut(s) 589
AvaII GGWCC 3 cut(s) 61, 97, 631
BaeGI GKGCMC 1 cut(s) 301
BbsI GAAGAC 2 cut(s) 366, 402
Bbv12I GWGCWC 1 cut(s) 301
BbvCI CCTCAGC 2 cut(s) 192, 269
BbvI GCAGC 2 cut(s) 214, 217
BccI CCATC 1 cut(s) 442
BceAI ACGGC 5 cut(s) 151, 157, 179, 268, 399
BcgI CGANNNNNNTGC 2 cut(s) 278, 312
BciT130I CCWGG 1 cut(s) 11
BcnI CCSGG 6 cut(s) 44, 77, 101, 590, 591, 635
BcoDI GTCTC 3 cut(s) 209, 532, 652
BfoI RGCGCY 1 cut(s) 228
BisI GCNGC 6 cut(s) 37, 228, 231, 369, 432, 458
BlsI GCNGC 6 cut(s) 38, 229, 232, 370, 433, 459
Bme1390I CCNGG 7 cut(s) 11, 44, 77, 101, 590, 591, 635
Bme18I GGWCC 3 cut(s) 61, 97, 631
BmeT110I CYCGRG 1 cut(s) 589
BmgT120I GGNCC 5 cut(s) 38, 61, 97, 440, 631
BmiI GGNNCC 3 cut(s) 40, 99, 441
BmrFI CCNGG 7 cut(s) 11, 44, 77, 101, 590, 591, 635
BpiI GAAGAC 2 cut(s) 366, 402
BplI GAGNNNNNCTC 3 cut(s) 585, 617, 686
BpmI CTGGAG 1 cut(s) 16
Bpu10I CCTNAGC 2 cut(s) 192, 269
Bpu14I TTCGAA 1 cut(s) 650
BpuMI CCSGG 6 cut(s) 44, 77, 101, 590, 591, 635
BsaHI GRCGYC 1 cut(s) 371
BsaI GGTCTC 2 cut(s) 532, 652
BsaJI CCNNGG 4 cut(s) 42, 57, 366, 589
Bsc4I CCNNNNNNNGG 3 cut(s) 16, 58, 293
Bse3DI GCAATG 2 cut(s) 684, 691
BseBI CCWGG 1 cut(s) 11
BseDI CCNNGG 4 cut(s) 42, 57, 366, 589
BseLI CCNNNNNNNGG 3 cut(s) 16, 58, 293
BseMI GCAATG 2 cut(s) 684, 691
BseMII CTCAG 5 cut(s) 206, 217, 225, 260, 548
BseRI GAGGAG 2 cut(s) 273, 276
BseSI GKGCMC 1 cut(s) 301
BseX3I CGGCCG 1 cut(s) 164
BseXI GCAGC 2 cut(s) 214, 217
BseYI CCCAGC 1 cut(s) 177
Bsh1236I CGCG 2 cut(s) 66, 368
Bsh1285I CGRYCG 1 cut(s) 167
BshFI GGCC 4 cut(s) 39, 166, 442, 457
BsiEI CGRYCG 1 cut(s) 167
BsiHKAI GWGCWC 1 cut(s) 301
BsiHKCI CYCGRG 1 cut(s) 589
BsiSI CCGG 5 cut(s) 44, 76, 101, 590, 634
BslI CCNNNNNNNGG 3 cut(s) 16, 58, 293
BsmAI GTCTC 3 cut(s) 209, 532, 652
BsnI GGCC 4 cut(s) 39, 166, 442, 457
Bso31I GGTCTC 2 cut(s) 532, 652
BsoBI CYCGRG 1 cut(s) 589
Bsp119I TTCGAA 1 cut(s) 650
Bsp1286I GDGCHC 1 cut(s) 301
Bsp143I GATC 1 cut(s) 6
BspANI GGCC 4 cut(s) 39, 166, 442, 457
BspCNI CTCAG 5 cut(s) 205, 216, 224, 261, 547
BspFNI CGCG 2 cut(s) 66, 368
BspLI GGNNCC 3 cut(s) 40, 99, 441
BspT104I TTCGAA 1 cut(s) 650
BspTNI GGTCTC 2 cut(s) 532, 652
BsrBI CCGCTC 1 cut(s) 423
BsrDI GCAATG 2 cut(s) 684, 691
BssECI CCNNGG 4 cut(s) 42, 57, 366, 589
BssMI GATC 1 cut(s) 6
BssNI GRCGYC 1 cut(s) 371
Bst2UI CCWGG 1 cut(s) 11
Bst4CI ACNGT 3 cut(s) 61, 124, 624
Bst6I CTCTTC 1 cut(s) 492
BstACI GRCGYC 1 cut(s) 371
BstBI TTCGAA 1 cut(s) 650
BstC8I GCNNGC 1 cut(s) 552
BstDEI CTNAG 5 cut(s) 192, 203, 211, 269, 534
BstDSI CCRYGG 2 cut(s) 57, 366
BstEII GGTNACC 1 cut(s) 46
BstFNI CGCG 2 cut(s) 66, 368
BstH2I RGCGCY 1 cut(s) 228
BstHHI GCGC 2 cut(s) 227, 388
BstKTI GATC 1 cut(s) 9
BstMAI GTCTC 3 cut(s) 209, 532, 652
BstMBI GATC 1 cut(s) 6
BstMCI CGRYCG 1 cut(s) 167
BstMWI GCNNNNNNNGC 3 cut(s) 72, 193, 402
BstNI CCWGG 1 cut(s) 11
BstPI GGTNACC 1 cut(s) 46
BstSCI CCNGG 7 cut(s) 9, 42, 75, 99, 588, 589, 633
BstSLI GKGCMC 1 cut(s) 301
BstUI CGCG 2 cut(s) 66, 368
BstV1I GCAGC 2 cut(s) 214, 217
BstV2I GAAGAC 2 cut(s) 366, 402
BstX2I RGATCY 1 cut(s) 6
BstYI RGATCY 1 cut(s) 6
BstZI CGGCCG 1 cut(s) 164
BsuRI GGCC 4 cut(s) 39, 166, 442, 457
BtgI CCRYGG 2 cut(s) 57, 366
Cac8I GCNNGC 1 cut(s) 552
CfoI GCGC 2 cut(s) 227, 388
Cfr13I GGNCC 5 cut(s) 38, 61, 97, 440, 631
Cfr42I CCGCGG 1 cut(s) 369
Cfr9I CCCGGG 1 cut(s) 589
CpoI CGGWCCG 1 cut(s) 61
CseI GACGC 3 cut(s) 55, 360, 622
Csp6I GTAC 1 cut(s) 625
CspI CGGWCCG 1 cut(s) 61
CviAII CATG 1 cut(s) 235
CviQI GTAC 1 cut(s) 625
DdeI CTNAG 5 cut(s) 192, 203, 211, 269, 534
DpnI GATC 1 cut(s) 8
DpnII GATC 1 cut(s) 6
EaeI YGGCCR 2 cut(s) 164, 455
EagI CGGCCG 1 cut(s) 164
Eam1104I CTCTTC 1 cut(s) 492
EarI CTCTTC 1 cut(s) 492
EciI GGCGGA 1 cut(s) 270
EclXI CGGCCG 1 cut(s) 164
Eco31I GGTCTC 2 cut(s) 532, 652
Eco47I GGWCC 3 cut(s) 61, 97, 631
Eco47III AGCGCT 1 cut(s) 226
Eco52I CGGCCG 1 cut(s) 164
Eco88I CYCGRG 1 cut(s) 589
Eco91I GGTNACC 1 cut(s) 46
EcoO109I RGGNCCY 1 cut(s) 97
EcoO65I GGTNACC 1 cut(s) 46
EcoRII CCWGG 1 cut(s) 9
FaeI CATG 1 cut(s) 238
FaiI YATR 3 cut(s) 236, 333, 509
FatI CATG 1 cut(s) 234
FauI CCCGC 1 cut(s) 398
Fnu4HI GCNGC 6 cut(s) 37, 228, 231, 369, 432, 458
Fsp4HI GCNGC 6 cut(s) 37, 228, 231, 369, 432, 458
GlaI GCGC 2 cut(s) 226, 387
GluI GCNGC 6 cut(s) 37, 228, 231, 369, 432, 458
GsaI CCCAGC 1 cut(s) 181
GsuI CTGGAG 1 cut(s) 16
HaeII RGCGCY 1 cut(s) 228
HaeIII GGCC 4 cut(s) 39, 166, 442, 457
HapII CCGG 5 cut(s) 44, 76, 101, 590, 634
HgaI GACGC 3 cut(s) 55, 360, 622
HhaI GCGC 2 cut(s) 227, 388
Hin1I GRCGYC 1 cut(s) 371
Hin1II CATG 1 cut(s) 238
Hin6I GCGC 2 cut(s) 225, 386
HinP1I GCGC 2 cut(s) 225, 386
HincII GTYRAC 1 cut(s) 161
HindII GTYRAC 1 cut(s) 161
HinfI GANTC 4 cut(s) 29, 106, 695, 701
HpaII CCGG 5 cut(s) 44, 76, 101, 590, 634
HphI GGTGA 1 cut(s) 25
Hpy166II GTNNAC 2 cut(s) 161, 299
Hpy188I TCNGA 8 cut(s) 132, 206, 214, 246, 379, 537, 700, 706
Hpy188III TCNNGA 1 cut(s) 469
Hpy8I GTNNAC 2 cut(s) 161, 299
Hpy99I CGWCG 1 cut(s) 418
HpyAV CCTTC 3 cut(s) 10, 16, 394
HpyCH4III ACNGT 3 cut(s) 61, 124, 624
HpyCH4V TGCA 1 cut(s) 299
HpyF10VI GCNNNNNNNGC 3 cut(s) 72, 193, 402
HpyF3I CTNAG 5 cut(s) 192, 203, 211, 269, 534
Hsp92I GRCGYC 1 cut(s) 371
Hsp92II CATG 1 cut(s) 238
HspAI GCGC 2 cut(s) 225, 386
KspI CCGCGG 1 cut(s) 369
Kzo9I GATC 1 cut(s) 6
LmnI GCTCC 2 cut(s) 420, 593
Lsp1109I GCAGC 2 cut(s) 214, 217
MaeIII GTNAC 2 cut(s) 46, 137
MalI GATC 1 cut(s) 8
MbiI CCGCTC 1 cut(s) 423
MboI GATC 1 cut(s) 6
MboII GAAGA 6 cut(s) 16, 366, 402, 509, 652, 655
MflI RGATCY 1 cut(s) 6
MhlI GDGCHC 1 cut(s) 301
MluCI AATT 2 cut(s) 276, 527
MlyI GAGTC 3 cut(s) 23, 695, 704
MmeI TCCRAC 1 cut(s) 678
MseI TTAA 3 cut(s) 126, 345, 671
MslI CAYNNNNRTG 2 cut(s) 333, 336
MspA1I CMGCKG 2 cut(s) 36, 368
MspI CCGG 5 cut(s) 44, 76, 101, 590, 634
MspR9I CCNGG 7 cut(s) 11, 44, 77, 101, 590, 591, 635
MvaI CCWGG 1 cut(s) 11
MvnI CGCG 2 cut(s) 66, 368
MwoI GCNNNNNNNGC 3 cut(s) 72, 193, 402
NciI CCSGG 6 cut(s) 44, 77, 101, 590, 591, 635
NdeII GATC 1 cut(s) 6
NlaIII CATG 1 cut(s) 238
NlaIV GGNNCC 3 cut(s) 40, 99, 441
NspV TTCGAA 1 cut(s) 650
PfeI GAWTC 1 cut(s) 106
PfoI TCCNGGA 1 cut(s) 633
PkrI GCNGC 6 cut(s) 38, 229, 232, 370, 433, 459
PleI GAGTC 3 cut(s) 23, 695, 703
PpsI GAGTC 3 cut(s) 23, 695, 703
PpuMI RGGWCCY 1 cut(s) 97
PshBI ATTAAT 1 cut(s) 345
Psp5II RGGWCCY 1 cut(s) 97
Psp6I CCWGG 1 cut(s) 9
PspEI GGTNACC 1 cut(s) 46
PspFI CCCAGC 1 cut(s) 177
PspGI CCWGG 1 cut(s) 9
PspN4I GGNNCC 3 cut(s) 40, 99, 441
PspPI GGNCC 5 cut(s) 38, 61, 97, 440, 631
PspPPI RGGWCCY 1 cut(s) 97
PsuI RGATCY 1 cut(s) 6
RsaI GTAC 1 cut(s) 626
RsaNI GTAC 1 cut(s) 625
RseI CAYNNNNRTG 2 cut(s) 333, 336
Rsr2I CGGWCCG 1 cut(s) 61
RsrII CGGWCCG 1 cut(s) 61
SacII CCGCGG 1 cut(s) 369
SaqAI TTAA 3 cut(s) 126, 345, 671
SatI GCNGC 6 cut(s) 37, 228, 231, 369, 432, 458
Sau3AI GATC 1 cut(s) 6
Sau96I GGNCC 5 cut(s) 38, 61, 97, 440, 631
SchI GAGTC 3 cut(s) 23, 695, 704
ScrFI CCNGG 7 cut(s) 11, 44, 77, 101, 590, 591, 635
SduI GDGCHC 1 cut(s) 301
SetI ASST 6 cut(s) 15, 114, 193, 244, 598, 633
Sfr303I CCGCGG 1 cut(s) 369
SfuI TTCGAA 1 cut(s) 650
SgrBI CCGCGG 1 cut(s) 369
SinI GGWCC 3 cut(s) 61, 97, 631
SmaI CCCGGG 1 cut(s) 591
SmiMI CAYNNNNRTG 2 cut(s) 333, 336
Sse9I AATT 2 cut(s) 276, 527
StyD4I CCNGG 7 cut(s) 9, 42, 75, 99, 588, 589, 633
TaaI ACNGT 3 cut(s) 61, 124, 624
TaqI TCGA 3 cut(s) 85, 524, 650
TasI AATT 2 cut(s) 276, 527
TauI GCSGC 4 cut(s) 39, 371, 434, 460
TfiI GAWTC 1 cut(s) 106
Tru1I TTAA 3 cut(s) 126, 345, 671
Tru9I TTAA 3 cut(s) 126, 345, 671
TseI GCWGC 2 cut(s) 227, 230
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 146
TspMI CCCGGG 1 cut(s) 589
VneI GTGCAC 1 cut(s) 297
VpaK11BI GGWCC 3 cut(s) 61, 97, 631
VspI ATTAAT 1 cut(s) 345
XcmI CCANNNNNNNNNTGG 1 cut(s) 450
XmaI CCCGGG 1 cut(s) 589
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.