Rmu_sc0020729.1_g000006

Vacuolar-processing enzyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0020729.1
OG0015556
Physical Location & Seq
Forward (+)
19483 .. 30897
11415 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0020729.1_g000006.1.cds

Sequence Viewer

Length: 2538 bp
atgactcggttggccaccgtcgtcgccgtccttcttttcttcctagtctccctctcttcttccgccaccagtagccgcgacctgatcggagacgtcttgcgcttgccgtcggaagcttcgaggttcttccgcgccggcgatggtggcgccgatcagcaagacgacggctccgtcggcacgcggtgggccgttttgatcgccggctccaatggatactggaattacaggcatcaggctgatatatgccatgcctatcaattattgaagaaaggcgggctgaaagatgaaaatattgttgtatttatgtatgatgatattgctcacaatgaagagaaccctaggcaaggagtcatcatcaacagtcctcacggtgatgatgtttataaaggagttccgaaggattacaccggggatgatgttactgttaataatttctttgctgctattctattgagtagcaacttagatgaaacgatttctcctgctttggggatgcctaccagtccctatatttacgcagacaggctcatagaagttctaaagaagaagcatgctgcagggacatatgaaagtttggtattttatcttgaagcttgtgaatccggaagtatctttgagggtcttcttcctgaaggtttgaatatctttgcaactacagcatcaaatgcagaagagagcagttgggggacttactgcccgggagagtatcctagtcctcccccagaatatgaaacctgtttgggtgacttgtatagtgttgcttggatggaagatagtgacgtacacaacttgaagtcagaaactttgcgccagcagtatgaactggtcaaatcaaggacagcaagtgataattctgcttatggttctcatgtcatgcaatatggcgacatagggctcaacaagaacaaccttttcatgtatatggggacaaatcctgcaaatgataactacactttcatgcctcagaacttcttcaggccatcttcaaaagctgtcaaccagagggatgctgatctggtccatttctgggataagttccgcaaggctcctgaaggctcaccaaggaaggctcaagcccaaaaagaatttcttgaagcaatgtctcacagaatgcatatagatgaaagcatgaaacttattgggaaactcctgtttggaatcaaaaaaggtccagaggtcctcagcgctgttcgacctgctgggcagccacttgttgatgactgggactgtcttaagacaatggtgaggagttttgagacgtattgcggatctctatctcagtatgggatgaaacacatgcgttccctggcaaacatctgcaacgctggaatgacgaaggagcagatggccgaggcttcagcccaagcctgcgtcaatgttccttccggccgatggagctctctgcacaggggattcagatctaattcgaagcttcgtctctgtgttggtgttgagagagaaaaccagaagctcggaaagatacattcgaggagtttcggaacgtattccaagtggaatcgagaggtgatcgagaatttcaagcttagaggcacatttgcaagagatttgagcggtttcgtgaagaatccttctggtgcgccagcagagcaagatttccgaaacgaagaagtttcttctgcgagtttaatcttagtaaatacaggtgtacaaaaggagatggaaagagactatgtgaggcacgccaatattaacgacaccaagaattctagagacagaaccggaggggatagctcactgctgttgagatcgagtcctgataaaacgacgacgtccgagttcgttttgcagtgggggaatcgcaagcgcctccggtgcatgaagatccaggtcaaggacgactccagtggtgtcccggttaccagaaccacggttcgggtagaccgccgggtcattcgaccggacaaggactcgtccaatcagccctctaccaccaataatagtaacgcgtatttgaacctccgtcaacgaccgttatcgccgcaacaaccaccacctcagcgtgttctcagaaactcggagacttcgagtgcaggcatgagaggccagagcaacggagctatgaggggaattatttcacctgacaggggcggcgttgcgcacgataagaaaggtacaactaacaaccacaatcatcaccaccaccataatgacaatcatcacaaccacaagtccgctgcgtcatcggagacggcgcacgatagcaagaagggcggctcctcatcggggagcggcgatgcggcagcagcaccgccgatggtgtggccgcccaagttcgtgattgccttgaccaacaaagagaaggaagaggatttcatggccattaagggatccaagctccctcagagacccaaaaagagagcaaagatcatccaacgccccctcaatctcgtaagcccgggtgcatggctgtgtgatctgacgctggaacggtacgaggtcagagagaagaagatctccaagaagagaccgagaggattgaaggcgatgggcaccatggtggagtcggactcggaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04612 map04142 map04612
Pfam Domains
Protein Families
Peptidase_C13

Protein Analysis

845

Amino Acids

94.05

Weight (kDa)

8.97

Isoelectric Point (pI)

48.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015556)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g32200
malus_domestica MD08G1073700.v1.1
prunus_persica Prupe.1G415100_v2.0.a1
pyrus_communis pycom08g06100 pycom15g05800
rosa_chinensis RchiOBHm_Chr6g0310471
rosa_laevigata RLG00000010480 RLG00000018734
rosa_multiflora Rmu_sc0020729.1_g000006
rosa_rugosa Rorug06G0380600
rosa_samantha Rh4DG040600 Rh6AG494600 Rh6BG504300 Rh6CG509500 Rh6DG495500
rosa_wichuraiana Rw6G043020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 384
AasI GACNNNNNNGTC 2 cut(s) 170, 1907
AatII GACGTC 2 cut(s) 96, 1793
Acc16I TGCGCA 1 cut(s) 2118
Acc36I ACCTGC 1 cut(s) 1204
AccB1I GGYRCC 2 cut(s) 146, 2510
AccBSI CCGCTC 2 cut(s) 1572, 2250
AccI GTMKAC 1 cut(s) 1899
AccII CGCG 4 cut(s) 78, 132, 181, 1967
AccIII TCCGGA 1 cut(s) 602
AclWI GGATC 4 cut(s) 1276, 1837, 2343, 2356
AcoI YGGCCR 5 cut(s) 12, 1347, 1387, 2282, 2337
AcsI RAATTY 3 cut(s) 1085, 1534, 1723
AcuI CTGAAG 4 cut(s) 651, 958, 1071, 1341
AcyI GRCGYC 3 cut(s) 93, 147, 1790
AdeI CACNNNGTG 1 cut(s) 183
AfaI GTAC 4 cut(s) 783, 1668, 2134, 2453
AfeI AGCGCT 1 cut(s) 1186
AfiI CCNNNNNNNGG 8 cut(s) 344, 488, 1027, 1392, 1852, 1893, 2105, 2244
AflII CTTAAG 1 cut(s) 1232
AflIII ACRYGT 1 cut(s) 1965
AgsI TTSAA 9 cut(s) 265, 590, 640, 793, 987, 1094, 1540, 1975, 2500
AjnI CCWGG 2 cut(s) 1305, 1845
AjuI GAANNNNNNNTTGG 2 cut(s) 1071, 1103
AleI CACNNNNGTG 1 cut(s) 2516
Alw21I GWGCWC 1 cut(s) 1400
AlwI GGATC 4 cut(s) 1276, 1837, 2343, 2356
Ama87I CYCGRG 2 cut(s) 697, 2416
Aor13HI TCCGGA 1 cut(s) 602
Aor51HI AGCGCT 1 cut(s) 1186
AoxI GGCC 8 cut(s) 12, 186, 977, 1347, 1387, 2062, 2282, 2337
ApeKI GCWGC 6 cut(s) 440, 554, 1204, 2195, 2261, 2264
ApoI RAATTY 3 cut(s) 1085, 1534, 1723
Asp700I GAANNNNTTC 3 cut(s) 971, 1504, 2092
AspA2I CCTAGG 1 cut(s) 338
AspLEI GCGC 9 cut(s) 102, 134, 149, 810, 1187, 1600, 1827, 2119, 2215
AspS9I GGNCC 4 cut(s) 186, 1018, 1169, 1177
AsuC2I CCSGG 7 cut(s) 409, 698, 699, 1874, 1907, 2417, 2418
AsuHPI GGTGA 7 cut(s) 383, 755, 1050, 1255, 1537, 2088, 2147
AsuII TTCGAA 1 cut(s) 1427
AvaI CYCGRG 2 cut(s) 697, 2416
AvaII GGWCC 3 cut(s) 1018, 1169, 1177
AvrII CCTAGG 1 cut(s) 338
BaeGI GKGCMC 1 cut(s) 2513
BalI TGGCCA 2 cut(s) 14, 2339
BamHI GGATCC 1 cut(s) 2348
BanI GGYRCC 2 cut(s) 146, 2510
BanII GRGCYC 2 cut(s) 897, 1400
BbsI GAAGAC 1 cut(s) 614
Bbv12I GWGCWC 1 cut(s) 1400
BbvCI CCTCAGC 2 cut(s) 1181, 2016
BbvI GCAGC 6 cut(s) 427, 541, 1216, 2182, 2273, 2276
BccI CCATC 8 cut(s) 134, 760, 988, 1339, 1386, 1672, 2269, 2500
BceAI ACGGC 5 cut(s) 11, 91, 173, 181, 2226
BciT130I CCWGG 2 cut(s) 1307, 1847
BciVI GTATCC 2 cut(s) 206, 717
BcnI CCSGG 7 cut(s) 409, 698, 699, 1874, 1907, 2417, 2418
BfaI CTAG 4 cut(s) 44, 339, 711, 1728
BfmI CTRYAG 2 cut(s) 555, 654
BfoI RGCGCY 3 cut(s) 150, 1188, 1828
BfrI CTTAAG 1 cut(s) 1232
BfuAI ACCTGC 1 cut(s) 1204
BfuI GTATCC 2 cut(s) 206, 717
BglII AGATCT 2 cut(s) 1418, 2472
BlnI CCTAGG 1 cut(s) 338
Bme1390I CCNGG 9 cut(s) 409, 698, 699, 1307, 1847, 1874, 1907, 2417, 2418
Bme18I GGWCC 3 cut(s) 1018, 1169, 1177
BmeT110I CYCGRG 2 cut(s) 697, 2416
BmgT120I GGNCC 4 cut(s) 186, 1018, 1169, 1177
BmiI GGNNCC 7 cut(s) 148, 169, 205, 1047, 2236, 2350, 2512
BmrFI CCNGG 9 cut(s) 409, 698, 699, 1307, 1847, 1874, 1907, 2417, 2418
BmrI ACTGGG 1 cut(s) 1231
BmsI GCATC 5 cut(s) 238, 483, 668, 997, 2245
BmuI ACTGGG 1 cut(s) 1231
BpiI GAAGAC 1 cut(s) 614
BplI GAGNNNNNCTC 1 cut(s) 2513
BpmI CTGGAG 1 cut(s) 1846
Bpu10I CCTNAGC 2 cut(s) 1181, 2016
Bpu14I TTCGAA 1 cut(s) 1427
BpuEI CTTGAG 1 cut(s) 1056
BpuMI CCSGG 7 cut(s) 409, 698, 699, 1874, 1907, 2417, 2418
BsaBI GATNNNNATC 3 cut(s) 1011, 1273, 1417
BsaHI GRCGYC 3 cut(s) 93, 147, 1790
BsaI GGTCTC 2 cut(s) 2359, 2479
BsaJI CCNNGG 9 cut(s) 338, 408, 697, 1061, 1305, 1350, 1887, 2416, 2514
BsaWI WCCGGW 4 cut(s) 602, 1739, 1830, 1918
BsaXI ACNNNNNCTCC 4 cut(s) 152, 182, 463, 493
Bsc4I CCNNNNNNNGG 8 cut(s) 344, 488, 1027, 1392, 1852, 1893, 2105, 2244
Bse118I RCCGGY 2 cut(s) 134, 200
Bse1I ACTGG 6 cut(s) 69, 221, 501, 828, 1226, 1863
Bse3DI GCAATG 1 cut(s) 1104
Bse8I GATNNNNATC 3 cut(s) 1011, 1273, 1417
BseAI TCCGGA 1 cut(s) 602
BseBI CCWGG 2 cut(s) 1307, 1847
BseDI CCNNGG 9 cut(s) 338, 408, 697, 1061, 1305, 1350, 1887, 2416, 2514
BseGI GGATG 6 cut(s) 418, 498, 771, 1012, 1293, 2388
BseJI GATNNNNATC 3 cut(s) 1011, 1273, 1417
BseLI CCNNNNNNNGG 8 cut(s) 344, 488, 1027, 1392, 1852, 1893, 2105, 2244
BseMI GCAATG 1 cut(s) 1104
BseMII CTCAG 6 cut(s) 977, 1195, 1292, 2030, 2041, 2375
BseNI ACTGG 6 cut(s) 69, 221, 501, 828, 1226, 1863
BseRI GAGGAG 3 cut(s) 1261, 1504, 2227
BseSI GKGCMC 1 cut(s) 2513
BseX3I CGGCCG 1 cut(s) 1387
BseXI GCAGC 6 cut(s) 427, 541, 1216, 2182, 2273, 2276
BseYI CCCAGC 1 cut(s) 1199
BsgI GTGCAG 2 cut(s) 1388, 2070
Bsh1236I CGCG 4 cut(s) 78, 132, 181, 1967
Bsh1285I CGRYCG 3 cut(s) 1390, 1919, 1991
BshFI GGCC 8 cut(s) 14, 188, 979, 1349, 1389, 2064, 2284, 2339
BshNI GGYRCC 2 cut(s) 146, 2510
BsiEI CGRYCG 3 cut(s) 1390, 1919, 1991
BsiHKAI GWGCWC 1 cut(s) 1400
BsiHKCI CYCGRG 2 cut(s) 697, 2416
BslFI GGGAC 6 cut(s) 489, 574, 700, 940, 1238, 1856
BslI CCNNNNNNNGG 8 cut(s) 344, 488, 1027, 1392, 1852, 1893, 2105, 2244
BsmBI CGTCTC 4 cut(s) 84, 1250, 1442, 2201
BsmFI GGGAC 6 cut(s) 489, 574, 700, 940, 1238, 1856
BsmI GAATGC 1 cut(s) 1116
BsnI GGCC 8 cut(s) 14, 188, 979, 1349, 1389, 2064, 2284, 2339
Bso31I GGTCTC 2 cut(s) 2359, 2479
BsoBI CYCGRG 2 cut(s) 697, 2416
Bsp119I TTCGAA 1 cut(s) 1427
Bsp1286I GDGCHC 3 cut(s) 897, 1400, 2513
Bsp13I TCCGGA 1 cut(s) 602
Bsp1407I TGTACA 1 cut(s) 1666
Bsp19I CCATGG 1 cut(s) 2514
BspANI GGCC 8 cut(s) 14, 188, 979, 1349, 1389, 2064, 2284, 2339
BspCNI CTCAG 6 cut(s) 976, 1194, 1291, 2029, 2040, 2374
BspEI TCCGGA 1 cut(s) 602
BspFNI CGCG 4 cut(s) 78, 132, 181, 1967
BspLI GGNNCC 7 cut(s) 148, 169, 205, 1047, 2236, 2350, 2512
BspMAI CTGCAG 1 cut(s) 559
BspMI ACCTGC 1 cut(s) 1204
BspPI GGATC 4 cut(s) 1276, 1837, 2343, 2356
BspT104I TTCGAA 1 cut(s) 1427
BspT107I GGYRCC 2 cut(s) 146, 2510
BspTI CTTAAG 1 cut(s) 1232
BspTNI GGTCTC 2 cut(s) 2359, 2479
BsrBI CCGCTC 2 cut(s) 1572, 2250
BsrDI GCAATG 1 cut(s) 1104
BsrFI RCCGGY 2 cut(s) 134, 200
BsrGI TGTACA 1 cut(s) 1666
BsrI ACTGG 6 cut(s) 69, 221, 501, 828, 1226, 1863
BssAI RCCGGY 2 cut(s) 134, 200
BssECI CCNNGG 9 cut(s) 338, 408, 697, 1061, 1305, 1350, 1887, 2416, 2514
BssNI GRCGYC 3 cut(s) 93, 147, 1790
BssT1I CCWWGG 3 cut(s) 338, 1061, 2514
Bst2UI CCWGG 2 cut(s) 1307, 1847
Bst4CI ACNGT 8 cut(s) 19, 362, 371, 424, 1229, 1891, 1992, 2451
Bst6I CTCTTC 5 cut(s) 61, 324, 666, 2319, 2477
BstACI GRCGYC 3 cut(s) 93, 147, 1790
BstAFI CTTAAG 1 cut(s) 1232
BstAPI GCANNNNNTGC 1 cut(s) 665
BstAUI TGTACA 1 cut(s) 1666
BstBI TTCGAA 1 cut(s) 1427
BstDEI CTNAG 9 cut(s) 463, 963, 1181, 1278, 1544, 1651, 2016, 2027, 2361
BstDSI CCRYGG 2 cut(s) 1887, 2514
BstEII GGTNACC 1 cut(s) 1876
BstENI CCTNNNNNAGG 1 cut(s) 342
BstF5I GGATG 6 cut(s) 418, 498, 771, 1012, 1293, 2388
BstFNI CGCG 4 cut(s) 78, 132, 181, 1967
BstH2I RGCGCY 3 cut(s) 150, 1188, 1828
BstHHI GCGC 9 cut(s) 102, 134, 149, 810, 1187, 1600, 1827, 2119, 2215
BstMCI CGRYCG 3 cut(s) 1390, 1919, 1991
BstNI CCWGG 2 cut(s) 1307, 1847
BstNSI RCATGY 2 cut(s) 554, 1300
BstPI GGTNACC 1 cut(s) 1876
BstSCI CCNGG 9 cut(s) 407, 696, 697, 1305, 1845, 1872, 1905, 2415, 2416
BstSFI CTRYAG 2 cut(s) 555, 654
BstSLI GKGCMC 1 cut(s) 2513
BstUI CGCG 4 cut(s) 78, 132, 181, 1967
BstV1I GCAGC 6 cut(s) 427, 541, 1216, 2182, 2273, 2276
BstV2I GAAGAC 1 cut(s) 614
BstX2I RGATCY 5 cut(s) 1268, 1418, 1842, 2348, 2472
BstYI RGATCY 5 cut(s) 1268, 1418, 1842, 2348, 2472
BstZI CGGCCG 1 cut(s) 1387
BsuI GTATCC 2 cut(s) 206, 717
BsuRI GGCC 8 cut(s) 14, 188, 979, 1349, 1389, 2064, 2284, 2339
BtgI CCRYGG 2 cut(s) 1887, 2514
BtgZI GCGATG 3 cut(s) 153, 2268, 2519
BtsCI GGATG 6 cut(s) 418, 498, 771, 1012, 1293, 2388
BtsI GCAGTG 2 cut(s) 1754, 1814
BtsIMutI CAGTG 3 cut(s) 1754, 1814, 1870
BveI ACCTGC 1 cut(s) 1204
CfoI GCGC 9 cut(s) 102, 134, 149, 810, 1187, 1600, 1827, 2119, 2215
Cfr10I RCCGGY 2 cut(s) 134, 200
Cfr13I GGNCC 4 cut(s) 186, 1018, 1169, 1177
Cfr9I CCCGGG 2 cut(s) 697, 2416
CseI GACGC 3 cut(s) 1360, 2187, 2449
Csp6I GTAC 4 cut(s) 782, 1667, 2133, 2452
CviQI GTAC 4 cut(s) 782, 1667, 2133, 2452
DdeI CTNAG 9 cut(s) 463, 963, 1181, 1278, 1544, 1651, 2016, 2027, 2361
DinI GGCGCC 1 cut(s) 148
DraIII CACNNNGTG 1 cut(s) 183
DrdI GACNNNNNNGTC 2 cut(s) 170, 1907
DseDI GACNNNNNNGTC 2 cut(s) 170, 1907
EaeI YGGCCR 5 cut(s) 12, 1347, 1387, 2282, 2337
EagI CGGCCG 1 cut(s) 1387
Eam1104I CTCTTC 5 cut(s) 61, 324, 666, 2319, 2477
EarI CTCTTC 5 cut(s) 61, 324, 666, 2319, 2477
EciI GGCGGA 1 cut(s) 52
Ecl136II GAGCTC 1 cut(s) 1398
EclXI CGGCCG 1 cut(s) 1387
Eco130I CCWWGG 3 cut(s) 338, 1061, 2514
Eco24I GRGCYC 2 cut(s) 897, 1400
Eco31I GGTCTC 2 cut(s) 2359, 2479
Eco47I GGWCC 3 cut(s) 1018, 1169, 1177
Eco47III AGCGCT 1 cut(s) 1186
Eco52I CGGCCG 1 cut(s) 1387
Eco53kI GAGCTC 1 cut(s) 1398
Eco57I CTGAAG 4 cut(s) 651, 958, 1071, 1341
Eco88I CYCGRG 2 cut(s) 697, 2416
Eco91I GGTNACC 1 cut(s) 1876
EcoICRI GAGCTC 1 cut(s) 1398
EcoNI CCTNNNNNAGG 1 cut(s) 342
EcoO109I RGGNCCY 1 cut(s) 1177
EcoO65I GGTNACC 1 cut(s) 1876
EcoRI GAATTC 1 cut(s) 1723
EcoRII CCWGG 2 cut(s) 1305, 1845
EcoT14I CCWWGG 3 cut(s) 338, 1061, 2514
EcoT22I ATGCAT 1 cut(s) 1116
EcoT38I GRGCYC 2 cut(s) 897, 1400
EgeI GGCGCC 1 cut(s) 148
EheI GGCGCC 1 cut(s) 148
ErhI CCWWGG 3 cut(s) 338, 1061, 2514
Esp3I CGTCTC 4 cut(s) 84, 1250, 1442, 2201
FalI AAGNNNNNCTT 2 cut(s) 1074, 1106
FaqI GGGAC 6 cut(s) 489, 574, 700, 940, 1238, 1856
FauI CCCGC 1 cut(s) 266
FauNDI CATATG 1 cut(s) 565
FblI GTMKAC 1 cut(s) 1899
FokI GGATG 6 cut(s) 425, 505, 778, 1019, 1300, 2375
FriOI GRGCYC 2 cut(s) 897, 1400
FspBI CTAG 4 cut(s) 44, 339, 711, 1728
FspI TGCGCA 1 cut(s) 2118
GlaI GCGC 9 cut(s) 101, 133, 148, 809, 1186, 1599, 1826, 2118, 2214
GsaI CCCAGC 1 cut(s) 1203
GsuI CTGGAG 1 cut(s) 1846
HaeII RGCGCY 3 cut(s) 150, 1188, 1828
HaeIII GGCC 8 cut(s) 14, 188, 979, 1349, 1389, 2064, 2284, 2339
HgaI GACGC 3 cut(s) 1360, 2187, 2449
HhaI GCGC 9 cut(s) 102, 134, 149, 810, 1187, 1600, 1827, 2119, 2215
Hin1I GRCGYC 3 cut(s) 93, 147, 1790
Hin6I GCGC 9 cut(s) 100, 132, 147, 808, 1185, 1598, 1825, 2117, 2213
HinP1I GCGC 9 cut(s) 100, 132, 147, 808, 1185, 1598, 1825, 2117, 2213
HincII GTYRAC 2 cut(s) 997, 1985
HindII GTYRAC 2 cut(s) 997, 1985
HindIII AAGCTT 4 cut(s) 114, 591, 1430, 1541
HphI GGTGA 7 cut(s) 383, 755, 1050, 1255, 1537, 2088, 2147
Hpy166II GTNNAC 5 cut(s) 784, 997, 1667, 1900, 1985
Hpy8I GTNNAC 5 cut(s) 784, 997, 1667, 1900, 1985
Hpy99I CGWCG 7 cut(s) 23, 26, 112, 167, 176, 1789, 1792
HpyCH4III ACNGT 8 cut(s) 19, 362, 371, 424, 1229, 1891, 1992, 2451
HpyCH4IV ACGT 5 cut(s) 93, 780, 1259, 1502, 1790
HpyF3I CTNAG 9 cut(s) 463, 963, 1181, 1278, 1544, 1651, 2016, 2027, 2361
HpySE526I ACGT 5 cut(s) 93, 780, 1259, 1502, 1790
Hsp92I GRCGYC 3 cut(s) 93, 147, 1790
HspAI GCGC 9 cut(s) 100, 132, 147, 808, 1185, 1598, 1825, 2117, 2213
KasI GGCGCC 1 cut(s) 146
Kpn2I TCCGGA 1 cut(s) 602
KroI GCCGGC 2 cut(s) 134, 200
KroNI GCCGGC 2 cut(s) 136, 202
LmnI GCTCC 9 cut(s) 173, 209, 1051, 1339, 1395, 2075, 2240, 2247, 2361
Lsp1109I GCAGC 6 cut(s) 427, 541, 1216, 2182, 2273, 2276
LweI GCATC 5 cut(s) 238, 483, 668, 997, 2245
MaeI CTAG 4 cut(s) 44, 339, 711, 1728
MaeII ACGT 5 cut(s) 93, 780, 1259, 1502, 1790
MaeIII GTNAC 5 cut(s) 418, 743, 776, 1876, 1961
MbiI CCGCTC 2 cut(s) 1572, 2250
MflI RGATCY 5 cut(s) 1268, 1418, 1842, 2348, 2472
MhlI GDGCHC 3 cut(s) 897, 1400, 2513
MlsI TGGCCA 2 cut(s) 14, 2339
MluCI AATT 9 cut(s) 220, 257, 430, 850, 1085, 1423, 1534, 1723, 2088
MluI ACGCGT 1 cut(s) 1965
MluNI TGGCCA 2 cut(s) 14, 2339
Mly113I GGCGCC 1 cut(s) 147
MlyI GAGTC 6 cut(s) 357, 1780, 1853, 1922, 2522, 2531
MmeI TCCRAC 3 cut(s) 90, 2416, 2505
Mox20I TGGCCA 2 cut(s) 14, 2339
Mph1103I ATGCAT 1 cut(s) 1116
MreI CGCCGGCG 1 cut(s) 134
MroI TCCGGA 1 cut(s) 602
MroNI GCCGGC 2 cut(s) 134, 200
MroXI GAANNNNTTC 3 cut(s) 971, 1504, 2092
MscI TGGCCA 2 cut(s) 14, 2339
MseI TTAA 5 cut(s) 426, 1233, 1646, 1710, 2343
MslI CAYNNNNRTG 7 cut(s) 372, 920, 956, 1119, 2166, 2428, 2516
Msp20I TGGCCA 2 cut(s) 14, 2339
MspA1I CMGCKG 1 cut(s) 2195
MspCI CTTAAG 1 cut(s) 1232
MspR9I CCNGG 9 cut(s) 409, 698, 699, 1307, 1847, 1874, 1907, 2417, 2418
Mva1269I GAATGC 1 cut(s) 1116
MvaI CCWGG 2 cut(s) 1307, 1847
MvnI CGCG 4 cut(s) 78, 132, 181, 1967
NaeI GCCGGC 2 cut(s) 136, 202
NarI GGCGCC 1 cut(s) 147
NciI CCSGG 7 cut(s) 409, 698, 699, 1874, 1907, 2417, 2418
NcoI CCATGG 1 cut(s) 2514
NdeI CATATG 1 cut(s) 565
NgoMIV GCCGGC 2 cut(s) 134, 200
NlaIV GGNNCC 7 cut(s) 148, 169, 205, 1047, 2236, 2350, 2512
NmeAIII GCCGAG 1 cut(s) 1375
NmuCI GTSAC 2 cut(s) 743, 776
NsbI TGCGCA 1 cut(s) 2118
NsiI ATGCAT 1 cut(s) 1116
NspI RCATGY 2 cut(s) 554, 1300
NspV TTCGAA 1 cut(s) 1427
OliI CACNNNNGTG 1 cut(s) 2516
PaeI GCATGC 1 cut(s) 554
PcsI WCGNNNNNNNCGW 3 cut(s) 116, 168, 2042
PctI GAATGC 1 cut(s) 1116
PdiI GCCGGC 2 cut(s) 136, 202
PdmI GAANNNNTTC 3 cut(s) 971, 1504, 2092
PfeI GAWTC 6 cut(s) 599, 1158, 1413, 1516, 1585, 1816
PflFI GACNNNGTC 1 cut(s) 1789
PleI GAGTC 6 cut(s) 356, 1779, 1853, 1922, 2522, 2530
PluTI GGCGCC 1 cut(s) 150
PpsI GAGTC 6 cut(s) 356, 1779, 1853, 1922, 2522, 2530
PpuMI RGGWCCY 1 cut(s) 1177
PsiI TTATAA 1 cut(s) 384
Psp124BI GAGCTC 1 cut(s) 1400
Psp5II RGGWCCY 1 cut(s) 1177
Psp6I CCWGG 2 cut(s) 1305, 1845
PspEI GGTNACC 1 cut(s) 1876
PspFI CCCAGC 1 cut(s) 1199
PspGI CCWGG 2 cut(s) 1305, 1845
PspN4I GGNNCC 7 cut(s) 148, 169, 205, 1047, 2236, 2350, 2512
PspPI GGNCC 4 cut(s) 186, 1018, 1169, 1177
PspPPI RGGWCCY 1 cut(s) 1177
PstI CTGCAG 1 cut(s) 559
PsuI RGATCY 5 cut(s) 1268, 1418, 1842, 2348, 2472
PsyI GACNNNGTC 1 cut(s) 1789
RsaI GTAC 4 cut(s) 783, 1668, 2134, 2453
RsaNI GTAC 4 cut(s) 782, 1667, 2133, 2452
RseI CAYNNNNRTG 7 cut(s) 372, 920, 956, 1119, 2166, 2428, 2516
SacI GAGCTC 1 cut(s) 1400
SaqAI TTAA 5 cut(s) 426, 1233, 1646, 1710, 2343
Sau96I GGNCC 4 cut(s) 186, 1018, 1169, 1177
SchI GAGTC 6 cut(s) 357, 1780, 1853, 1922, 2522, 2531
ScrFI CCNGG 9 cut(s) 409, 698, 699, 1307, 1847, 1874, 1907, 2417, 2418
SduI GDGCHC 3 cut(s) 897, 1400, 2513
SfaNI GCATC 5 cut(s) 238, 483, 668, 997, 2245
SfcI CTRYAG 2 cut(s) 555, 654
SfoI GGCGCC 1 cut(s) 148
SfuI TTCGAA 1 cut(s) 1427
SgrAI CRCCGGYG 1 cut(s) 134
SinI GGWCC 3 cut(s) 1018, 1169, 1177
SmaI CCCGGG 2 cut(s) 699, 2418
SmiMI CAYNNNNRTG 7 cut(s) 372, 920, 956, 1119, 2166, 2428, 2516
SmlI CTYRAG 2 cut(s) 1071, 1232
SmoI CTYRAG 2 cut(s) 1071, 1232
SphI GCATGC 1 cut(s) 554
Sse9I AATT 9 cut(s) 220, 257, 430, 850, 1085, 1423, 1534, 1723, 2088
SspDI GGCGCC 1 cut(s) 146
SspI AATATT 2 cut(s) 292, 1708
SspMI CTAG 4 cut(s) 44, 339, 711, 1728
SstI GAGCTC 1 cut(s) 1400
StyD4I CCNGG 9 cut(s) 407, 696, 697, 1305, 1845, 1872, 1905, 2415, 2416
StyI CCWWGG 3 cut(s) 338, 1061, 2514
TaaI ACNGT 8 cut(s) 19, 362, 371, 424, 1229, 1891, 1992, 2451
TaiI ACGT 5 cut(s) 96, 783, 1262, 1505, 1793
TaqI TCGA 9 cut(s) 119, 1192, 1427, 1487, 1519, 1530, 1769, 1915, 2045
TaqII GACCGA 1 cut(s) 2503
TasI AATT 9 cut(s) 220, 257, 430, 850, 1085, 1423, 1534, 1723, 2088
TatI WGTACW 1 cut(s) 1666
TauI GCSGC 7 cut(s) 78, 2002, 2112, 2235, 2253, 2261, 2287
TfiI GAWTC 6 cut(s) 599, 1158, 1413, 1516, 1585, 1816
Tru1I TTAA 5 cut(s) 426, 1233, 1646, 1710, 2343
Tru9I TTAA 5 cut(s) 426, 1233, 1646, 1710, 2343
TscAI CASTG 3 cut(s) 1761, 1814, 1870
TseFI GTSAC 2 cut(s) 743, 776
TseI GCWGC 6 cut(s) 440, 554, 1204, 2195, 2261, 2264
Tsp45I GTSAC 2 cut(s) 743, 776
TspGWI ACGGA 3 cut(s) 160, 1970, 2088
TspMI CCCGGG 2 cut(s) 697, 2416
TspRI CASTG 3 cut(s) 1761, 1814, 1870
Tth111I GACNNNGTC 1 cut(s) 1789
Vha464I CTTAAG 1 cut(s) 1232
VpaK11BI GGWCC 3 cut(s) 1018, 1169, 1177
XagI CCTNNNNNAGG 1 cut(s) 342
XapI RAATTY 3 cut(s) 1085, 1534, 1723
XbaI TCTAGA 1 cut(s) 1727
XceI RCATGY 2 cut(s) 554, 1300
XmaI CCCGGG 2 cut(s) 697, 2416
XmaJI CCTAGG 1 cut(s) 338
XmiI GTMKAC 1 cut(s) 1899
XmnI GAANNNNTTC 3 cut(s) 971, 1504, 2092
XspI CTAG 4 cut(s) 44, 339, 711, 1728
ZraI GACGTC 2 cut(s) 94, 1791
Zsp2I ATGCAT 1 cut(s) 1116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.