pycom08g06100

Protein of unknown function (DUF1639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0015556
Physical Location & Seq
Forward (+)
4658858 .. 4664723
5866 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g06100.4

Sequence Viewer

Length: 852 bp
ATGCAGATGGAAAGGGACTGTACCAGCAGTAGAGATAGAACCGGTGGGGACGCATCCGCCTCCGTCTCCGCCTCGGTTGAAGTCCGACACCAAACGACGTCGGATTTCGTCTTGCAGTGGGGGAACCGCAAGCGCCTCCGGTGTATGAAGATCCAGGTGAAGGAGAAGGACGACTCCAGCGGCCCTCCGGTTACTCGAACCACGGTCCGCATCAGTCGCCGGGTTGTTCGACCCGATAAGGACCCGGCGAATCACCTTTCCACCACCGTTAATCAGACGAGTAACGGCTATTTGAATATCCGTCAACGGCCGTTATCTCCCCAGCAACCGCTACCTCAGCGTATTCTCAGAAACTCAGAGACTACGAGCGCTGCTGCCATGAGAGGCCAAAGCAACGGCGGAGTAGGAGGGCTGAGGGGAATTGCCTCGCCCGACAGGGGTGCGCACGATAAGAAAAACCACCACCACCACCATAATGATGAGACTAATCATCACAATAACAAGTCCGCGGCGTCTTCGGACACAGCGCACGATAGCAAGAAGGGCGGGTCTTCGTCGGGGAGCGGAGAGGCGGCGGCGGGGCCACAGATGGTGTGGCCGCCGAAGTTCGTGATTGCTTTGACCAATAAGGAGAAAGAAGAGGACTTTATGGCAATCAAGGGGTCGAAATTGCCTCAGAGACCAAAGAAGCGAGCCAAGTTCGTTCAACGCACCCTCAATCTTGTAAGCCCGGGAGCTTGGCTTTGTGATTTGACGCTGGAACGGTATGAGGTCCGGGAGAAGAAGATTTCGAAAAAGAGACCAAGAGGGTTAAAGGCAATGGGCAATGTGGTTGAGTCGGACTCTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1639

Protein Analysis

284

Amino Acids

31.27

Weight (kDa)

10.16

Isoelectric Point (pI)

45.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015556)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g32200
malus_domestica MD08G1073700.v1.1
prunus_persica Prupe.1G415100_v2.0.a1
pyrus_communis pycom08g06100 pycom15g05800
rosa_chinensis RchiOBHm_Chr6g0310471
rosa_laevigata RLG00000010480 RLG00000018734
rosa_multiflora Rmu_sc0020729.1_g000006
rosa_rugosa Rorug06G0380600
rosa_samantha Rh4DG040600 Rh6AG494600 Rh6BG504300 Rh6CG509500 Rh6DG495500
rosa_wichuraiana Rw6G043020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 101
Acc16I TGCGCA 1 cut(s) 444
AccBSI CCGCTC 1 cut(s) 564
AccII CGCG 1 cut(s) 509
AclWI GGATC 1 cut(s) 145
AcoI YGGCCR 2 cut(s) 308, 596
AcyI GRCGYC 2 cut(s) 98, 512
AfaI GTAC 1 cut(s) 22
AfeI AGCGCT 1 cut(s) 370
AfiI CCNNNNNNNGG 2 cut(s) 160, 437
AgeI ACCGGT 1 cut(s) 41
AgsI TTSAA 3 cut(s) 80, 295, 707
AjnI CCWGG 1 cut(s) 153
AluBI AGCT 1 cut(s) 737
AluI AGCT 1 cut(s) 737
Alw26I GTCTC 5 cut(s) 70, 353, 476, 673, 793
AlwI GGATC 1 cut(s) 145
Ama87I CYCGRG 1 cut(s) 730
Aor51HI AGCGCT 1 cut(s) 370
AoxI GGCC 5 cut(s) 181, 308, 385, 581, 596
ApeKI GCWGC 2 cut(s) 371, 374
AsiGI ACCGGT 1 cut(s) 41
AspLEI GCGC 4 cut(s) 135, 371, 445, 529
AspS9I GGNCC 5 cut(s) 182, 205, 241, 581, 772
AsuC2I CCSGG 5 cut(s) 221, 245, 731, 732, 776
AsuHPI GGTGA 2 cut(s) 169, 245
AsuII TTCGAA 1 cut(s) 791
AvaI CYCGRG 1 cut(s) 730
AvaII GGWCC 3 cut(s) 205, 241, 772
BbsI GAAGAC 2 cut(s) 507, 543
BbvCI CCTCAGC 2 cut(s) 336, 413
BbvI GCAGC 2 cut(s) 358, 361
BccI CCATC 1 cut(s) 583
BceAI ACGGC 4 cut(s) 295, 301, 323, 412
BcgI CGANNNNNNTGC 2 cut(s) 422, 456
BciT130I CCWGG 1 cut(s) 155
BcnI CCSGG 5 cut(s) 221, 245, 731, 732, 776
BcoDI GTCTC 5 cut(s) 70, 353, 476, 673, 793
BfoI RGCGCY 2 cut(s) 136, 372
BisI GCNGC 7 cut(s) 181, 372, 375, 510, 573, 576, 599
BlsI GCNGC 7 cut(s) 182, 373, 376, 511, 574, 577, 600
Bme1390I CCNGG 6 cut(s) 155, 221, 245, 731, 732, 776
Bme18I GGWCC 3 cut(s) 205, 241, 772
BmeT110I CYCGRG 1 cut(s) 730
BmgT120I GGNCC 5 cut(s) 182, 205, 241, 581, 772
BmiI GGNNCC 3 cut(s) 125, 243, 582
BmrFI CCNGG 6 cut(s) 155, 221, 245, 731, 732, 776
BmsI GCATC 2 cut(s) 62, 219
BpiI GAAGAC 2 cut(s) 507, 543
BplI GAGNNNNNCTC 1 cut(s) 827
BpmI CTGGAG 1 cut(s) 160
Bpu10I CCTNAGC 2 cut(s) 336, 413
Bpu14I TTCGAA 1 cut(s) 791
BpuMI CCSGG 5 cut(s) 221, 245, 731, 732, 776
BsaHI GRCGYC 2 cut(s) 98, 512
BsaI GGTCTC 2 cut(s) 673, 793
BsaJI CCNNGG 4 cut(s) 72, 201, 507, 730
BsaWI WCCGGW 3 cut(s) 41, 138, 187
Bsc4I CCNNNNNNNGG 2 cut(s) 160, 437
Bse118I RCCGGY 1 cut(s) 41
Bse3DI GCAATG 2 cut(s) 825, 832
BseBI CCWGG 1 cut(s) 155
BseDI CCNNGG 4 cut(s) 72, 201, 507, 730
BseGI GGATG 1 cut(s) 53
BseLI CCNNNNNNNGG 2 cut(s) 160, 437
BseMI GCAATG 2 cut(s) 825, 832
BseMII CTCAG 5 cut(s) 350, 361, 369, 404, 689
BseX3I CGGCCG 1 cut(s) 308
BseXI GCAGC 2 cut(s) 358, 361
BseYI CCCAGC 1 cut(s) 321
Bsh1236I CGCG 1 cut(s) 509
Bsh1285I CGRYCG 1 cut(s) 311
BshFI GGCC 5 cut(s) 183, 310, 387, 583, 598
BshTI ACCGGT 1 cut(s) 41
BsiEI CGRYCG 1 cut(s) 311
BsiHKCI CYCGRG 1 cut(s) 730
BsiSI CCGG 7 cut(s) 42, 139, 188, 220, 245, 731, 775
BslFI GGGAC 2 cut(s) 29, 62
BslI CCNNNNNNNGG 2 cut(s) 160, 437
BsmAI GTCTC 5 cut(s) 70, 353, 476, 673, 793
BsmBI CGTCTC 1 cut(s) 70
BsmFI GGGAC 2 cut(s) 29, 62
BsnI GGCC 5 cut(s) 183, 310, 387, 583, 598
Bso31I GGTCTC 2 cut(s) 673, 793
BsoBI CYCGRG 1 cut(s) 730
Bsp119I TTCGAA 1 cut(s) 791
Bsp143I GATC 1 cut(s) 150
BspANI GGCC 5 cut(s) 183, 310, 387, 583, 598
BspCNI CTCAG 5 cut(s) 349, 360, 368, 405, 688
BspFNI CGCG 1 cut(s) 509
BspLI GGNNCC 3 cut(s) 125, 243, 582
BspPI GGATC 1 cut(s) 145
BspT104I TTCGAA 1 cut(s) 791
BspTNI GGTCTC 2 cut(s) 673, 793
BsrBI CCGCTC 1 cut(s) 564
BsrDI GCAATG 2 cut(s) 825, 832
BsrFI RCCGGY 1 cut(s) 41
BssAI RCCGGY 1 cut(s) 41
BssECI CCNNGG 4 cut(s) 72, 201, 507, 730
BssMI GATC 1 cut(s) 150
BssNI GRCGYC 2 cut(s) 98, 512
Bst2UI CCWGG 1 cut(s) 155
Bst4CI ACNGT 4 cut(s) 20, 205, 268, 765
Bst6I CTCTTC 1 cut(s) 633
BstACI GRCGYC 2 cut(s) 98, 512
BstBI TTCGAA 1 cut(s) 791
BstC8I GCNNGC 2 cut(s) 131, 693
BstDEI CTNAG 5 cut(s) 336, 347, 355, 413, 675
BstDSI CCRYGG 2 cut(s) 201, 507
BstF5I GGATG 1 cut(s) 53
BstFNI CGCG 1 cut(s) 509
BstH2I RGCGCY 2 cut(s) 136, 372
BstHHI GCGC 4 cut(s) 135, 371, 445, 529
BstKTI GATC 1 cut(s) 153
BstMAI GTCTC 5 cut(s) 70, 353, 476, 673, 793
BstMBI GATC 1 cut(s) 150
BstMCI CGRYCG 1 cut(s) 311
BstMWI GCNNNNNNNGC 3 cut(s) 216, 337, 543
BstNI CCWGG 1 cut(s) 155
BstSCI CCNGG 6 cut(s) 153, 219, 243, 729, 730, 774
BstUI CGCG 1 cut(s) 509
BstV1I GCAGC 2 cut(s) 358, 361
BstV2I GAAGAC 2 cut(s) 507, 543
BstX2I RGATCY 1 cut(s) 150
BstYI RGATCY 1 cut(s) 150
BstZI CGGCCG 1 cut(s) 308
BsuRI GGCC 5 cut(s) 183, 310, 387, 583, 598
BtgI CCRYGG 2 cut(s) 201, 507
BtsCI GGATG 1 cut(s) 53
BtsI GCAGTG 1 cut(s) 122
BtsIMutI CAGTG 1 cut(s) 122
Cac8I GCNNGC 2 cut(s) 131, 693
CfoI GCGC 4 cut(s) 135, 371, 445, 529
Cfr10I RCCGGY 1 cut(s) 41
Cfr13I GGNCC 5 cut(s) 182, 205, 241, 581, 772
Cfr42I CCGCGG 1 cut(s) 510
Cfr9I CCCGGG 1 cut(s) 730
CpoI CGGWCCG 1 cut(s) 205
CseI GACGC 3 cut(s) 59, 501, 763
Csp6I GTAC 1 cut(s) 21
CspAI ACCGGT 1 cut(s) 41
CspI CGGWCCG 1 cut(s) 205
CviAII CATG 1 cut(s) 379
CviQI GTAC 1 cut(s) 21
DdeI CTNAG 5 cut(s) 336, 347, 355, 413, 675
DpnI GATC 1 cut(s) 152
DpnII GATC 1 cut(s) 150
EaeI YGGCCR 2 cut(s) 308, 596
EagI CGGCCG 1 cut(s) 308
Eam1104I CTCTTC 1 cut(s) 633
EarI CTCTTC 1 cut(s) 633
EciI GGCGGA 3 cut(s) 46, 58, 414
EclXI CGGCCG 1 cut(s) 308
Eco31I GGTCTC 2 cut(s) 673, 793
Eco47I GGWCC 3 cut(s) 205, 241, 772
Eco47III AGCGCT 1 cut(s) 370
Eco52I CGGCCG 1 cut(s) 308
Eco88I CYCGRG 1 cut(s) 730
EcoO109I RGGNCCY 1 cut(s) 241
EcoRII CCWGG 1 cut(s) 153
Esp3I CGTCTC 1 cut(s) 70
FaeI CATG 1 cut(s) 382
FaiI YATR 5 cut(s) 146, 380, 474, 650, 768
FaqI GGGAC 2 cut(s) 29, 62
FatI CATG 1 cut(s) 378
FauI CCCGC 2 cut(s) 539, 571
Fnu4HI GCNGC 7 cut(s) 181, 372, 375, 510, 573, 576, 599
FokI GGATG 1 cut(s) 40
Fsp4HI GCNGC 7 cut(s) 181, 372, 375, 510, 573, 576, 599
FspAI RTGCGCAY 1 cut(s) 444
FspI TGCGCA 1 cut(s) 444
GlaI GCGC 4 cut(s) 134, 370, 444, 528
GluI GCNGC 7 cut(s) 181, 372, 375, 510, 573, 576, 599
GsaI CCCAGC 1 cut(s) 325
GsuI CTGGAG 1 cut(s) 160
HaeII RGCGCY 2 cut(s) 136, 372
HaeIII GGCC 5 cut(s) 183, 310, 387, 583, 598
HapII CCGG 7 cut(s) 42, 139, 188, 220, 245, 731, 775
HgaI GACGC 3 cut(s) 59, 501, 763
HhaI GCGC 4 cut(s) 135, 371, 445, 529
Hin1I GRCGYC 2 cut(s) 98, 512
Hin1II CATG 1 cut(s) 382
Hin6I GCGC 4 cut(s) 133, 369, 443, 527
HinP1I GCGC 4 cut(s) 133, 369, 443, 527
HincII GTYRAC 1 cut(s) 305
HindII GTYRAC 1 cut(s) 305
HinfI GANTC 4 cut(s) 173, 250, 836, 842
HpaII CCGG 7 cut(s) 42, 139, 188, 220, 245, 731, 775
HphI GGTGA 2 cut(s) 169, 245
Hpy166II GTNNAC 1 cut(s) 305
Hpy188I TCNGA 9 cut(s) 86, 103, 276, 350, 358, 520, 678, 841, 847
Hpy188III TCNNGA 1 cut(s) 610
Hpy8I GTNNAC 1 cut(s) 305
Hpy99I CGWCG 3 cut(s) 100, 103, 559
HpyAV CCTTC 3 cut(s) 154, 160, 535
HpyCH4III ACNGT 4 cut(s) 20, 205, 268, 765
HpyCH4IV ACGT 1 cut(s) 98
HpyCH4V TGCA 2 cut(s) 4, 115
HpyF10VI GCNNNNNNNGC 3 cut(s) 216, 337, 543
HpyF3I CTNAG 5 cut(s) 336, 347, 355, 413, 675
HpySE526I ACGT 1 cut(s) 98
Hsp92I GRCGYC 2 cut(s) 98, 512
Hsp92II CATG 1 cut(s) 382
HspAI GCGC 4 cut(s) 133, 369, 443, 527
KspI CCGCGG 1 cut(s) 510
Kzo9I GATC 1 cut(s) 150
LmnI GCTCC 2 cut(s) 561, 734
Lsp1109I GCAGC 2 cut(s) 358, 361
LweI GCATC 2 cut(s) 62, 219
MaeII ACGT 1 cut(s) 98
MaeIII GTNAC 2 cut(s) 190, 281
MalI GATC 1 cut(s) 152
MbiI CCGCTC 1 cut(s) 564
MboI GATC 1 cut(s) 150
MboII GAAGA 6 cut(s) 160, 507, 543, 650, 793, 796
MflI RGATCY 1 cut(s) 150
MluCI AATT 2 cut(s) 420, 668
MlyI GAGTC 3 cut(s) 167, 836, 845
MmeI TCCRAC 3 cut(s) 81, 109, 819
MseI TTAA 2 cut(s) 270, 812
MslI CAYNNNNRTG 2 cut(s) 474, 477
MspA1I CMGCKG 2 cut(s) 180, 509
MspI CCGG 7 cut(s) 42, 139, 188, 220, 245, 731, 775
MspR9I CCNGG 6 cut(s) 155, 221, 245, 731, 732, 776
MvaI CCWGG 1 cut(s) 155
MvnI CGCG 1 cut(s) 509
MwoI GCNNNNNNNGC 3 cut(s) 216, 337, 543
NciI CCSGG 5 cut(s) 221, 245, 731, 732, 776
NdeII GATC 1 cut(s) 150
NlaIII CATG 1 cut(s) 382
NlaIV GGNNCC 3 cut(s) 125, 243, 582
NsbI TGCGCA 1 cut(s) 444
NspV TTCGAA 1 cut(s) 791
PfeI GAWTC 1 cut(s) 250
PfoI TCCNGGA 1 cut(s) 774
PinAI ACCGGT 1 cut(s) 41
PkrI GCNGC 7 cut(s) 182, 373, 376, 511, 574, 577, 600
PleI GAGTC 3 cut(s) 167, 836, 844
PpsI GAGTC 3 cut(s) 167, 836, 844
PpuMI RGGWCCY 1 cut(s) 241
Psp5II RGGWCCY 1 cut(s) 241
Psp6I CCWGG 1 cut(s) 153
PspFI CCCAGC 1 cut(s) 321
PspGI CCWGG 1 cut(s) 153
PspN4I GGNNCC 3 cut(s) 125, 243, 582
PspPI GGNCC 5 cut(s) 182, 205, 241, 581, 772
PspPPI RGGWCCY 1 cut(s) 241
PsuI RGATCY 1 cut(s) 150
RsaI GTAC 1 cut(s) 22
RsaNI GTAC 1 cut(s) 21
RseI CAYNNNNRTG 2 cut(s) 474, 477
Rsr2I CGGWCCG 1 cut(s) 205
RsrII CGGWCCG 1 cut(s) 205
SacII CCGCGG 1 cut(s) 510
SaqAI TTAA 2 cut(s) 270, 812
SatI GCNGC 7 cut(s) 181, 372, 375, 510, 573, 576, 599
Sau3AI GATC 1 cut(s) 150
Sau96I GGNCC 5 cut(s) 182, 205, 241, 581, 772
SchI GAGTC 3 cut(s) 167, 836, 845
ScrFI CCNGG 6 cut(s) 155, 221, 245, 731, 732, 776
SetI ASST 6 cut(s) 101, 159, 258, 337, 739, 774
SfaNI GCATC 2 cut(s) 62, 219
Sfr303I CCGCGG 1 cut(s) 510
SfuI TTCGAA 1 cut(s) 791
SgrBI CCGCGG 1 cut(s) 510
SinI GGWCC 3 cut(s) 205, 241, 772
SmaI CCCGGG 1 cut(s) 732
SmiMI CAYNNNNRTG 2 cut(s) 474, 477
Sse9I AATT 2 cut(s) 420, 668
StyD4I CCNGG 6 cut(s) 153, 219, 243, 729, 730, 774
TaaI ACNGT 4 cut(s) 20, 205, 268, 765
TaiI ACGT 1 cut(s) 101
TaqI TCGA 4 cut(s) 196, 229, 665, 791
TasI AATT 2 cut(s) 420, 668
TauI GCSGC 5 cut(s) 183, 512, 575, 578, 601
TfiI GAWTC 1 cut(s) 250
Tru1I TTAA 2 cut(s) 270, 812
Tru9I TTAA 2 cut(s) 270, 812
TscAI CASTG 1 cut(s) 122
TseI GCWGC 2 cut(s) 371, 374
TspDTI ATGAA 1 cut(s) 161
TspGWI ACGGA 2 cut(s) 52, 290
TspMI CCCGGG 1 cut(s) 730
TspRI CASTG 1 cut(s) 122
VpaK11BI GGWCC 3 cut(s) 205, 241, 772
XcmI CCANNNNNNNNNTGG 1 cut(s) 591
XmaI CCCGGG 1 cut(s) 730
ZraI GACGTC 1 cut(s) 99
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.