Rh6BG504300

Protein of unknown function (DUF1639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0015556
Physical Location & Seq
Reverse (-)
70284911 .. 70288390
3480 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG504300.1

Sequence Viewer

Length: 867 bp
ATGGAAAGAGACTATGTGAGGCACGCCAATATTAACGACACCAAGAATTCTAGAGACAGAACCGGAGGGGATAGCTCACTGCTGTTGAGATCGAGTCCTGATAAAACGACGACGTCGGAGTTCGTTTTGCAGTGGGGGAATCGCAAGCGCCTCCGGTGCATGAAGATCCAGGTCAAGGACGACTCCAGTGGTGTCCCGGTTACCAGAACCACGGTTCGGGTAGACCGCCGGGTCATTCGACCGGACAAGGACTCGTCCAATCAGCCCTCTACCACCAATAATAGTAACGGGTATTTGAACCTCCGTCAACGACCGTTATCGCCGCAACAACCACCACCTCAGCGTGTTCTCAGAAACTCGGAGACTTCAAGTGCAGGCATGAGAGGCCAGAGCAACGGAGCTATGAGGGGAATTATTTCGCCTGACAGGGGCGGCGTTGCGCACGATAAGAAAGGTACAACTAACAACCACAACCACAATCATCACCATCACCATAATGACAATCATCACAACCACAAGTCCGCTGCGTCATCGGAGACGGCGCACGATAGCAAGAAGGGCGGCTCCTCATCGGGGAGCGGCGACGCGGCAGCAGCACCGCCGATGGTGTGGCCGCCCAAGTTCGTGATTGCCTTGACCAACAAAGAGAAGGAAGAGGATTTCATGGCCATTAAGGGATCCAAGCTCCCTCAGAGACCCAAAAAGAGAGCAAAGATCATCCAACGCACCCTCAATCTCGTAAGCCCGGGTGCATGGCTGTGTGATCTGACGCTGGAACGGTACGAGGTCAGAGAGAAGAAGATCTCCAAGAAGAGACCGAGAGGATTGAAGGCGATGGGCACCATGGTGGAGTCGGACTCGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1639

Protein Analysis

288

Amino Acids

32.11

Weight (kDa)

10.27

Isoelectric Point (pI)

51.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1639 PF07797 212 - 261 5.5e-23 Protein of unknown function (DUF1639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015556)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g32200
malus_domestica MD08G1073700.v1.1
prunus_persica Prupe.1G415100_v2.0.a1
pyrus_communis pycom08g06100 pycom15g05800
rosa_chinensis RchiOBHm_Chr6g0310471
rosa_laevigata RLG00000010480 RLG00000018734
rosa_multiflora Rmu_sc0020729.1_g000006
rosa_rugosa Rorug06G0380600
rosa_samantha Rh4DG040600 Rh6AG494600 Rh6BG504300 Rh6CG509500 Rh6DG495500
rosa_wichuraiana Rw6G043020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 230
AatII GACGTC 1 cut(s) 116
Acc16I TGCGCA 1 cut(s) 441
AccB1I GGYRCC 1 cut(s) 839
AccBSI CCGCTC 1 cut(s) 579
AccI GTMKAC 1 cut(s) 222
AccII CGCG 1 cut(s) 587
AciI CCGC 9 cut(s) 226, 323, 432, 522, 561, 579, 587, 599, 614
AclWI GGATC 3 cut(s) 160, 672, 685
AcoI YGGCCR 2 cut(s) 611, 666
AcsI RAATTY 1 cut(s) 46
AcyI GRCGYC 1 cut(s) 113
AfaI GTAC 2 cut(s) 457, 782
AfiI CCNNNNNNNGG 4 cut(s) 175, 216, 428, 573
AgsI TTSAA 3 cut(s) 298, 369, 829
AjnI CCWGG 1 cut(s) 168
AleI CACNNNNGTG 1 cut(s) 845
AluBI AGCT 3 cut(s) 75, 401, 685
AluI AGCT 3 cut(s) 75, 401, 685
Alw26I GTCTC 6 cut(s) 3, 48, 356, 530, 688, 808
AlwI GGATC 3 cut(s) 160, 672, 685
Ama87I CYCGRG 1 cut(s) 745
AoxI GGCC 3 cut(s) 385, 611, 666
ApeKI GCWGC 3 cut(s) 524, 590, 593
ApoI RAATTY 1 cut(s) 46
Asp700I GAANNNNTTC 1 cut(s) 415
AspLEI GCGC 3 cut(s) 150, 442, 544
AsuC2I CCSGG 4 cut(s) 197, 230, 746, 747
AsuHPI GGTGA 2 cut(s) 476, 482
AvaI CYCGRG 1 cut(s) 745
BaeGI GKGCMC 1 cut(s) 842
BalI TGGCCA 1 cut(s) 668
BamHI GGATCC 1 cut(s) 677
BanI GGYRCC 1 cut(s) 839
BbvCI CCTCAGC 1 cut(s) 339
BbvI GCAGC 3 cut(s) 511, 602, 605
BccI CCATC 3 cut(s) 495, 598, 829
BceAI ACGGC 1 cut(s) 555
BciT130I CCWGG 1 cut(s) 170
BcnI CCSGG 4 cut(s) 197, 230, 746, 747
BcoDI GTCTC 6 cut(s) 3, 48, 356, 530, 688, 808
BfaI CTAG 1 cut(s) 51
BfoI RGCGCY 1 cut(s) 151
BglII AGATCT 1 cut(s) 801
BisI GCNGC 9 cut(s) 323, 433, 525, 562, 580, 588, 591, 594, 614
BlsI GCNGC 9 cut(s) 324, 434, 526, 563, 581, 589, 592, 595, 615
Bme1390I CCNGG 5 cut(s) 170, 197, 230, 746, 747
BmeT110I CYCGRG 1 cut(s) 745
BmiI GGNNCC 3 cut(s) 565, 679, 841
BmrFI CCNGG 5 cut(s) 170, 197, 230, 746, 747
BplI GAGNNNNNCTC 1 cut(s) 842
BpmI CTGGAG 1 cut(s) 169
Bpu10I CCTNAGC 1 cut(s) 339
BpuMI CCSGG 4 cut(s) 197, 230, 746, 747
BsaHI GRCGYC 1 cut(s) 113
BsaI GGTCTC 2 cut(s) 688, 808
BsaJI CCNNGG 3 cut(s) 210, 745, 843
BsaWI WCCGGW 3 cut(s) 62, 153, 241
Bsc4I CCNNNNNNNGG 4 cut(s) 175, 216, 428, 573
Bse1I ACTGG 1 cut(s) 186
BseBI CCWGG 1 cut(s) 170
BseDI CCNNGG 3 cut(s) 210, 745, 843
BseGI GGATG 1 cut(s) 717
BseLI CCNNNNNNNGG 4 cut(s) 175, 216, 428, 573
BseMII CTCAG 3 cut(s) 353, 364, 704
BseNI ACTGG 1 cut(s) 186
BseRI GAGGAG 1 cut(s) 556
BseSI GKGCMC 1 cut(s) 842
BseXI GCAGC 3 cut(s) 511, 602, 605
BsgI GTGCAG 1 cut(s) 393
Bsh1236I CGCG 1 cut(s) 587
Bsh1285I CGRYCG 2 cut(s) 242, 314
BshFI GGCC 3 cut(s) 387, 613, 668
BshNI GGYRCC 1 cut(s) 839
BsiEI CGRYCG 2 cut(s) 242, 314
BsiHKCI CYCGRG 1 cut(s) 745
BsiSI CCGG 6 cut(s) 63, 154, 197, 229, 242, 746
BslFI GGGAC 1 cut(s) 179
BslI CCNNNNNNNGG 4 cut(s) 175, 216, 428, 573
BsmAI GTCTC 6 cut(s) 3, 48, 356, 530, 688, 808
BsmBI CGTCTC 1 cut(s) 530
BsmFI GGGAC 1 cut(s) 179
BsnI GGCC 3 cut(s) 387, 613, 668
Bso31I GGTCTC 2 cut(s) 688, 808
BsoBI CYCGRG 1 cut(s) 745
Bsp1286I GDGCHC 1 cut(s) 842
Bsp143I GATC 6 cut(s) 89, 165, 677, 714, 763, 801
Bsp19I CCATGG 1 cut(s) 843
BspACI CCGC 9 cut(s) 226, 323, 432, 522, 561, 579, 587, 599, 614
BspANI GGCC 3 cut(s) 387, 613, 668
BspCNI CTCAG 3 cut(s) 352, 363, 703
BspFNI CGCG 1 cut(s) 587
BspLI GGNNCC 3 cut(s) 565, 679, 841
BspPI GGATC 3 cut(s) 160, 672, 685
BspT107I GGYRCC 1 cut(s) 839
BspTNI GGTCTC 2 cut(s) 688, 808
BsrBI CCGCTC 1 cut(s) 579
BsrI ACTGG 1 cut(s) 186
BssECI CCNNGG 3 cut(s) 210, 745, 843
BssMI GATC 6 cut(s) 89, 165, 677, 714, 763, 801
BssNI GRCGYC 1 cut(s) 113
BssT1I CCWWGG 1 cut(s) 843
Bst2UI CCWGG 1 cut(s) 170
Bst4CI ACNGT 3 cut(s) 214, 315, 780
Bst6I CTCTTC 2 cut(s) 648, 806
BstACI GRCGYC 1 cut(s) 113
BstC8I GCNNGC 3 cut(s) 24, 146, 376
BstDEI CTNAG 3 cut(s) 339, 350, 690
BstDSI CCRYGG 2 cut(s) 210, 843
BstEII GGTNACC 1 cut(s) 199
BstF5I GGATG 1 cut(s) 717
BstFNI CGCG 1 cut(s) 587
BstH2I RGCGCY 1 cut(s) 151
BstHHI GCGC 3 cut(s) 150, 442, 544
BstKTI GATC 6 cut(s) 92, 168, 680, 717, 766, 804
BstMAI GTCTC 6 cut(s) 3, 48, 356, 530, 688, 808
BstMBI GATC 6 cut(s) 89, 165, 677, 714, 763, 801
BstMCI CGRYCG 2 cut(s) 242, 314
BstMWI GCNNNNNNNGC 4 cut(s) 156, 384, 558, 593
BstNI CCWGG 1 cut(s) 170
BstPI GGTNACC 1 cut(s) 199
BstSCI CCNGG 5 cut(s) 168, 195, 228, 744, 745
BstSLI GKGCMC 1 cut(s) 842
BstUI CGCG 1 cut(s) 587
BstV1I GCAGC 3 cut(s) 511, 602, 605
BstX2I RGATCY 3 cut(s) 165, 677, 801
BstYI RGATCY 3 cut(s) 165, 677, 801
BsuRI GGCC 3 cut(s) 387, 613, 668
BtgI CCRYGG 2 cut(s) 210, 843
BtgZI GCGATG 1 cut(s) 848
BtsCI GGATG 1 cut(s) 717
BtsI GCAGTG 2 cut(s) 77, 137
BtsIMutI CAGTG 3 cut(s) 77, 137, 193
Cac8I GCNNGC 3 cut(s) 24, 146, 376
CfoI GCGC 3 cut(s) 150, 442, 544
Cfr9I CCCGGG 1 cut(s) 745
CseI GACGC 3 cut(s) 516, 593, 778
Csp6I GTAC 2 cut(s) 456, 781
CviAII CATG 5 cut(s) 160, 379, 664, 753, 844
CviQI GTAC 2 cut(s) 456, 781
DdeI CTNAG 3 cut(s) 339, 350, 690
DpnI GATC 6 cut(s) 91, 167, 679, 716, 765, 803
DpnII GATC 6 cut(s) 89, 165, 677, 714, 763, 801
DrdI GACNNNNNNGTC 1 cut(s) 230
DseDI GACNNNNNNGTC 1 cut(s) 230
EaeI YGGCCR 2 cut(s) 611, 666
Eam1104I CTCTTC 2 cut(s) 648, 806
EarI CTCTTC 2 cut(s) 648, 806
Eco130I CCWWGG 1 cut(s) 843
Eco31I GGTCTC 2 cut(s) 688, 808
Eco88I CYCGRG 1 cut(s) 745
Eco91I GGTNACC 1 cut(s) 199
EcoO65I GGTNACC 1 cut(s) 199
EcoRI GAATTC 1 cut(s) 46
EcoRII CCWGG 1 cut(s) 168
EcoT14I CCWWGG 1 cut(s) 843
ErhI CCWWGG 1 cut(s) 843
Esp3I CGTCTC 1 cut(s) 530
FaeI CATG 5 cut(s) 163, 382, 667, 756, 847
FaiI YATR 8 cut(s) 15, 161, 380, 404, 495, 665, 754, 845
FaqI GGGAC 1 cut(s) 179
FatI CATG 5 cut(s) 159, 378, 663, 752, 843
FblI GTMKAC 1 cut(s) 222
Fnu4HI GCNGC 9 cut(s) 323, 433, 525, 562, 580, 588, 591, 594, 614
FokI GGATG 1 cut(s) 704
Fsp4HI GCNGC 9 cut(s) 323, 433, 525, 562, 580, 588, 591, 594, 614
FspBI CTAG 1 cut(s) 51
FspI TGCGCA 1 cut(s) 441
GlaI GCGC 3 cut(s) 149, 441, 543
GluI GCNGC 9 cut(s) 323, 433, 525, 562, 580, 588, 591, 594, 614
GsuI CTGGAG 1 cut(s) 169
HaeII RGCGCY 1 cut(s) 151
HaeIII GGCC 3 cut(s) 387, 613, 668
HapII CCGG 6 cut(s) 63, 154, 197, 229, 242, 746
HgaI GACGC 3 cut(s) 516, 593, 778
HhaI GCGC 3 cut(s) 150, 442, 544
Hin1I GRCGYC 1 cut(s) 113
Hin1II CATG 5 cut(s) 163, 382, 667, 756, 847
Hin6I GCGC 3 cut(s) 148, 440, 542
HinP1I GCGC 3 cut(s) 148, 440, 542
HincII GTYRAC 1 cut(s) 308
HindII GTYRAC 1 cut(s) 308
HinfI GANTC 6 cut(s) 94, 139, 182, 251, 851, 857
HpaII CCGG 6 cut(s) 63, 154, 197, 229, 242, 746
HphI GGTGA 2 cut(s) 476, 482
Hpy166II GTNNAC 2 cut(s) 223, 308
Hpy188I TCNGA 9 cut(s) 118, 353, 361, 535, 693, 768, 791, 856, 862
Hpy188III TCNNGA 3 cut(s) 51, 98, 625
Hpy8I GTNNAC 2 cut(s) 223, 308
Hpy99I CGWCG 4 cut(s) 112, 115, 118, 587
HpyAV CCTTC 3 cut(s) 550, 643, 823
HpyCH4III ACNGT 3 cut(s) 214, 315, 780
HpyCH4IV ACGT 1 cut(s) 113
HpyCH4V TGCA 4 cut(s) 130, 159, 374, 752
HpyF10VI GCNNNNNNNGC 4 cut(s) 156, 384, 558, 593
HpyF3I CTNAG 3 cut(s) 339, 350, 690
HpySE526I ACGT 1 cut(s) 113
Hsp92I GRCGYC 1 cut(s) 113
Hsp92II CATG 5 cut(s) 163, 382, 667, 756, 847
HspAI GCGC 3 cut(s) 148, 440, 542
Kzo9I GATC 6 cut(s) 89, 165, 677, 714, 763, 801
LmnI GCTCC 4 cut(s) 398, 569, 576, 690
Lsp1109I GCAGC 3 cut(s) 511, 602, 605
MaeI CTAG 1 cut(s) 51
MaeII ACGT 1 cut(s) 113
MaeIII GTNAC 2 cut(s) 199, 284
MalI GATC 6 cut(s) 91, 167, 679, 716, 765, 803
MbiI CCGCTC 1 cut(s) 579
MboI GATC 6 cut(s) 89, 165, 677, 714, 763, 801
MboII GAAGA 5 cut(s) 175, 665, 808, 811, 823
MflI RGATCY 3 cut(s) 165, 677, 801
MhlI GDGCHC 1 cut(s) 842
MlsI TGGCCA 1 cut(s) 668
MluCI AATT 2 cut(s) 46, 411
MluNI TGGCCA 1 cut(s) 668
MlyI GAGTC 5 cut(s) 103, 176, 245, 851, 860
MmeI TCCRAC 3 cut(s) 96, 745, 834
Mox20I TGGCCA 1 cut(s) 668
MroXI GAANNNNTTC 1 cut(s) 415
MscI TGGCCA 1 cut(s) 668
MseI TTAA 2 cut(s) 33, 672
MslI CAYNNNNRTG 3 cut(s) 495, 757, 845
Msp20I TGGCCA 1 cut(s) 668
MspA1I CMGCKG 1 cut(s) 524
MspI CCGG 6 cut(s) 63, 154, 197, 229, 242, 746
MspR9I CCNGG 5 cut(s) 170, 197, 230, 746, 747
MvaI CCWGG 1 cut(s) 170
MvnI CGCG 1 cut(s) 587
MwoI GCNNNNNNNGC 4 cut(s) 156, 384, 558, 593
NciI CCSGG 4 cut(s) 197, 230, 746, 747
NcoI CCATGG 1 cut(s) 843
NdeII GATC 6 cut(s) 89, 165, 677, 714, 763, 801
NlaIII CATG 5 cut(s) 163, 382, 667, 756, 847
NlaIV GGNNCC 3 cut(s) 565, 679, 841
NsbI TGCGCA 1 cut(s) 441
OliI CACNNNNGTG 1 cut(s) 845
PdmI GAANNNNTTC 1 cut(s) 415
PfeI GAWTC 1 cut(s) 139
PflFI GACNNNGTC 1 cut(s) 112
PkrI GCNGC 9 cut(s) 324, 434, 526, 563, 581, 589, 592, 595, 615
PleI GAGTC 5 cut(s) 102, 176, 245, 851, 859
PpsI GAGTC 5 cut(s) 102, 176, 245, 851, 859
Psp6I CCWGG 1 cut(s) 168
PspEI GGTNACC 1 cut(s) 199
PspGI CCWGG 1 cut(s) 168
PspN4I GGNNCC 3 cut(s) 565, 679, 841
PsuI RGATCY 3 cut(s) 165, 677, 801
PsyI GACNNNGTC 1 cut(s) 112
RsaI GTAC 2 cut(s) 457, 782
RsaNI GTAC 2 cut(s) 456, 781
RseI CAYNNNNRTG 3 cut(s) 495, 757, 845
SaqAI TTAA 2 cut(s) 33, 672
SatI GCNGC 9 cut(s) 323, 433, 525, 562, 580, 588, 591, 594, 614
Sau3AI GATC 6 cut(s) 89, 165, 677, 714, 763, 801
SchI GAGTC 5 cut(s) 103, 176, 245, 851, 860
ScrFI CCNGG 5 cut(s) 170, 197, 230, 746, 747
SduI GDGCHC 1 cut(s) 842
SetI ASST 9 cut(s) 77, 116, 174, 303, 340, 403, 457, 687, 789
SmaI CCCGGG 1 cut(s) 747
SmiMI CAYNNNNRTG 3 cut(s) 495, 757, 845
Sse9I AATT 2 cut(s) 46, 411
SsiI CCGC 9 cut(s) 226, 323, 432, 522, 561, 579, 587, 599, 614
SspI AATATT 1 cut(s) 31
SspMI CTAG 1 cut(s) 51
StyD4I CCNGG 5 cut(s) 168, 195, 228, 744, 745
StyI CCWWGG 1 cut(s) 843
TaaI ACNGT 3 cut(s) 214, 315, 780
TaiI ACGT 1 cut(s) 116
TaqI TCGA 2 cut(s) 92, 238
TaqII GACCGA 1 cut(s) 832
TasI AATT 2 cut(s) 46, 411
TauI GCSGC 6 cut(s) 325, 435, 564, 582, 590, 616
TfiI GAWTC 1 cut(s) 139
Tru1I TTAA 2 cut(s) 33, 672
Tru9I TTAA 2 cut(s) 33, 672
TscAI CASTG 3 cut(s) 84, 137, 193
TseI GCWGC 3 cut(s) 524, 590, 593
TspDTI ATGAA 2 cut(s) 176, 652
TspGWI ACGGA 2 cut(s) 293, 411
TspMI CCCGGG 1 cut(s) 745
TspRI CASTG 3 cut(s) 84, 137, 193
Tth111I GACNNNGTC 1 cut(s) 112
XapI RAATTY 1 cut(s) 46
XbaI TCTAGA 1 cut(s) 50
XmaI CCCGGG 1 cut(s) 745
XmiI GTMKAC 1 cut(s) 222
XmnI GAANNNNTTC 1 cut(s) 415
XspI CTAG 1 cut(s) 51
ZraI GACGTC 1 cut(s) 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.