Rh6DG495500

Protein of unknown function (DUF1639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0015556
Physical Location & Seq
Reverse (-)
65367671 .. 65373151
5481 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG495500.1

Sequence Viewer

Length: 870 bp
ATGGAAAGAGACTATGTGAGGCACGCCAATATTAACGACGCCAAGAATTCTAGAGACAGAACCGGAGGGGATAGCTCACTGCTGTTGAGATCGAGTCCTGATAAAACGACGACGACGTCGGAGTTCGTTTTGCAGTGGGGGAATCGCAAGCGCCTCCGGTGCATGAAGATCCAGGTCAAGGACGACTCCAGTGGTGTCCCGGTTACCAGAACCACGGTTCGGGTAGACCGCCGGGTCATTCGACCGGACAAGGACTCGTCCAATCAGCCCTCTACCACCAATAATAGTAACGGGTATTTGAACCTCCGTCAACGACCGTTATCGCCGCAACAACCACCACCTCAGCGTGTTCTCAGAAACTCGGAGACTTCAAGTGCAGGCATGAGAGGCCAGAGCAACGGAGCTATGAGGGGAATTATTTCGCCTGACAGGGGCGGCGTTGCGCACGATAAGAAAGGTACAACTAACAACCACAACCACAATCATCACCATCACCATAATGACAATCATCACAACCACAAGTCCGCTGCGTCATCGGAGACGGCGCACGATAGCAAGAAGGGCGGCTCCTCATCGGGGAGCGGCGACGCGGCAGCAGCACCGCCGATGGTGTGGCCGCCCAAGTTCGTGATTGCCTTGACCAACAAAGAGAAGGAAGAGGATTTCATGGCCATTAAGGGATCCAAGCTCCCTCAGAGACCCAAAAAGAGAGCAAAGATCATCCAACGCACCCTCAATCTCGTAAGCCCGGGTGCATGGCTGTGTGATCTGACGCTGGAACGGTACGAGGTCAGAGAGAAGAAGATCTCCAAGAAGAGACCGAGAGGATTGAAGGCGATGGGCACCATGGTGGAGTCGGACTCGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1639

Protein Analysis

289

Amino Acids

32.18

Weight (kDa)

10.27

Isoelectric Point (pI)

52.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1639 PF07797 213 - 262 5.5e-23 Protein of unknown function (DUF1639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015556)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g32200
malus_domestica MD08G1073700.v1.1
prunus_persica Prupe.1G415100_v2.0.a1
pyrus_communis pycom08g06100 pycom15g05800
rosa_chinensis RchiOBHm_Chr6g0310471
rosa_laevigata RLG00000010480 RLG00000018734
rosa_multiflora Rmu_sc0020729.1_g000006
rosa_rugosa Rorug06G0380600
rosa_samantha Rh4DG040600 Rh6AG494600 Rh6BG504300 Rh6CG509500 Rh6DG495500
rosa_wichuraiana Rw6G043020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 115, 233
AatII GACGTC 1 cut(s) 119
Acc16I TGCGCA 1 cut(s) 444
AccB1I GGYRCC 1 cut(s) 842
AccBSI CCGCTC 1 cut(s) 582
AccI GTMKAC 1 cut(s) 225
AccII CGCG 1 cut(s) 590
AciI CCGC 9 cut(s) 229, 326, 435, 525, 564, 582, 590, 602, 617
AclWI GGATC 3 cut(s) 163, 675, 688
AcoI YGGCCR 2 cut(s) 614, 669
AcsI RAATTY 1 cut(s) 46
AcyI GRCGYC 2 cut(s) 39, 116
AfaI GTAC 2 cut(s) 460, 785
AfiI CCNNNNNNNGG 4 cut(s) 178, 219, 431, 576
AgsI TTSAA 3 cut(s) 301, 372, 832
AjnI CCWGG 1 cut(s) 171
AleI CACNNNNGTG 1 cut(s) 848
AluBI AGCT 3 cut(s) 75, 404, 688
AluI AGCT 3 cut(s) 75, 404, 688
Alw26I GTCTC 6 cut(s) 3, 48, 359, 533, 691, 811
AlwI GGATC 3 cut(s) 163, 675, 688
Ama87I CYCGRG 1 cut(s) 748
AoxI GGCC 3 cut(s) 388, 614, 669
ApeKI GCWGC 3 cut(s) 527, 593, 596
ApoI RAATTY 1 cut(s) 46
Asp700I GAANNNNTTC 1 cut(s) 418
AspLEI GCGC 3 cut(s) 153, 445, 547
AsuC2I CCSGG 4 cut(s) 200, 233, 749, 750
AsuHPI GGTGA 2 cut(s) 479, 485
AvaI CYCGRG 1 cut(s) 748
BaeGI GKGCMC 1 cut(s) 845
BalI TGGCCA 1 cut(s) 671
BamHI GGATCC 1 cut(s) 680
BanI GGYRCC 1 cut(s) 842
BbvCI CCTCAGC 1 cut(s) 342
BbvI GCAGC 3 cut(s) 514, 605, 608
BccI CCATC 3 cut(s) 498, 601, 832
BceAI ACGGC 1 cut(s) 558
BciT130I CCWGG 1 cut(s) 173
BcnI CCSGG 4 cut(s) 200, 233, 749, 750
BcoDI GTCTC 6 cut(s) 3, 48, 359, 533, 691, 811
BfaI CTAG 1 cut(s) 51
BfoI RGCGCY 1 cut(s) 154
BglII AGATCT 1 cut(s) 804
BisI GCNGC 9 cut(s) 326, 436, 528, 565, 583, 591, 594, 597, 617
BlsI GCNGC 9 cut(s) 327, 437, 529, 566, 584, 592, 595, 598, 618
Bme1390I CCNGG 5 cut(s) 173, 200, 233, 749, 750
BmeT110I CYCGRG 1 cut(s) 748
BmiI GGNNCC 3 cut(s) 568, 682, 844
BmrFI CCNGG 5 cut(s) 173, 200, 233, 749, 750
BplI GAGNNNNNCTC 1 cut(s) 845
BpmI CTGGAG 1 cut(s) 172
Bpu10I CCTNAGC 1 cut(s) 342
BpuMI CCSGG 4 cut(s) 200, 233, 749, 750
BsaHI GRCGYC 2 cut(s) 39, 116
BsaI GGTCTC 2 cut(s) 691, 811
BsaJI CCNNGG 3 cut(s) 213, 748, 846
BsaWI WCCGGW 3 cut(s) 62, 156, 244
Bsc4I CCNNNNNNNGG 4 cut(s) 178, 219, 431, 576
Bse1I ACTGG 1 cut(s) 189
BseBI CCWGG 1 cut(s) 173
BseDI CCNNGG 3 cut(s) 213, 748, 846
BseGI GGATG 1 cut(s) 720
BseLI CCNNNNNNNGG 4 cut(s) 178, 219, 431, 576
BseMII CTCAG 3 cut(s) 356, 367, 707
BseNI ACTGG 1 cut(s) 189
BseRI GAGGAG 1 cut(s) 559
BseSI GKGCMC 1 cut(s) 845
BseXI GCAGC 3 cut(s) 514, 605, 608
BsgI GTGCAG 1 cut(s) 396
Bsh1236I CGCG 1 cut(s) 590
Bsh1285I CGRYCG 2 cut(s) 245, 317
BshFI GGCC 3 cut(s) 390, 616, 671
BshNI GGYRCC 1 cut(s) 842
BsiEI CGRYCG 2 cut(s) 245, 317
BsiHKCI CYCGRG 1 cut(s) 748
BsiSI CCGG 6 cut(s) 63, 157, 200, 232, 245, 749
BslFI GGGAC 1 cut(s) 182
BslI CCNNNNNNNGG 4 cut(s) 178, 219, 431, 576
BsmAI GTCTC 6 cut(s) 3, 48, 359, 533, 691, 811
BsmBI CGTCTC 1 cut(s) 533
BsmFI GGGAC 1 cut(s) 182
BsnI GGCC 3 cut(s) 390, 616, 671
Bso31I GGTCTC 2 cut(s) 691, 811
BsoBI CYCGRG 1 cut(s) 748
Bsp1286I GDGCHC 1 cut(s) 845
Bsp143I GATC 6 cut(s) 89, 168, 680, 717, 766, 804
Bsp19I CCATGG 1 cut(s) 846
BspACI CCGC 9 cut(s) 229, 326, 435, 525, 564, 582, 590, 602, 617
BspANI GGCC 3 cut(s) 390, 616, 671
BspCNI CTCAG 3 cut(s) 355, 366, 706
BspFNI CGCG 1 cut(s) 590
BspLI GGNNCC 3 cut(s) 568, 682, 844
BspPI GGATC 3 cut(s) 163, 675, 688
BspT107I GGYRCC 1 cut(s) 842
BspTNI GGTCTC 2 cut(s) 691, 811
BsrBI CCGCTC 1 cut(s) 582
BsrI ACTGG 1 cut(s) 189
BssECI CCNNGG 3 cut(s) 213, 748, 846
BssMI GATC 6 cut(s) 89, 168, 680, 717, 766, 804
BssNI GRCGYC 2 cut(s) 39, 116
BssT1I CCWWGG 1 cut(s) 846
Bst2UI CCWGG 1 cut(s) 173
Bst4CI ACNGT 3 cut(s) 217, 318, 783
Bst6I CTCTTC 2 cut(s) 651, 809
BstACI GRCGYC 2 cut(s) 39, 116
BstC8I GCNNGC 3 cut(s) 24, 149, 379
BstDEI CTNAG 3 cut(s) 342, 353, 693
BstDSI CCRYGG 2 cut(s) 213, 846
BstEII GGTNACC 1 cut(s) 202
BstF5I GGATG 1 cut(s) 720
BstFNI CGCG 1 cut(s) 590
BstH2I RGCGCY 1 cut(s) 154
BstHHI GCGC 3 cut(s) 153, 445, 547
BstKTI GATC 6 cut(s) 92, 171, 683, 720, 769, 807
BstMAI GTCTC 6 cut(s) 3, 48, 359, 533, 691, 811
BstMBI GATC 6 cut(s) 89, 168, 680, 717, 766, 804
BstMCI CGRYCG 2 cut(s) 245, 317
BstMWI GCNNNNNNNGC 4 cut(s) 159, 387, 561, 596
BstNI CCWGG 1 cut(s) 173
BstPI GGTNACC 1 cut(s) 202
BstSCI CCNGG 5 cut(s) 171, 198, 231, 747, 748
BstSLI GKGCMC 1 cut(s) 845
BstUI CGCG 1 cut(s) 590
BstV1I GCAGC 3 cut(s) 514, 605, 608
BstX2I RGATCY 3 cut(s) 168, 680, 804
BstYI RGATCY 3 cut(s) 168, 680, 804
BsuRI GGCC 3 cut(s) 390, 616, 671
BtgI CCRYGG 2 cut(s) 213, 846
BtgZI GCGATG 1 cut(s) 851
BtsCI GGATG 1 cut(s) 720
BtsI GCAGTG 2 cut(s) 77, 140
BtsIMutI CAGTG 3 cut(s) 77, 140, 196
Cac8I GCNNGC 3 cut(s) 24, 149, 379
CfoI GCGC 3 cut(s) 153, 445, 547
Cfr9I CCCGGG 1 cut(s) 748
CseI GACGC 4 cut(s) 47, 519, 596, 781
Csp6I GTAC 2 cut(s) 459, 784
CviAII CATG 5 cut(s) 163, 382, 667, 756, 847
CviQI GTAC 2 cut(s) 459, 784
DdeI CTNAG 3 cut(s) 342, 353, 693
DpnI GATC 6 cut(s) 91, 170, 682, 719, 768, 806
DpnII GATC 6 cut(s) 89, 168, 680, 717, 766, 804
DrdI GACNNNNNNGTC 2 cut(s) 115, 233
DseDI GACNNNNNNGTC 2 cut(s) 115, 233
EaeI YGGCCR 2 cut(s) 614, 669
Eam1104I CTCTTC 2 cut(s) 651, 809
EarI CTCTTC 2 cut(s) 651, 809
Eco130I CCWWGG 1 cut(s) 846
Eco31I GGTCTC 2 cut(s) 691, 811
Eco88I CYCGRG 1 cut(s) 748
Eco91I GGTNACC 1 cut(s) 202
EcoO65I GGTNACC 1 cut(s) 202
EcoRI GAATTC 1 cut(s) 46
EcoRII CCWGG 1 cut(s) 171
EcoT14I CCWWGG 1 cut(s) 846
ErhI CCWWGG 1 cut(s) 846
Esp3I CGTCTC 1 cut(s) 533
FaeI CATG 5 cut(s) 166, 385, 670, 759, 850
FaiI YATR 8 cut(s) 15, 164, 383, 407, 498, 668, 757, 848
FaqI GGGAC 1 cut(s) 182
FatI CATG 5 cut(s) 162, 381, 666, 755, 846
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 9 cut(s) 326, 436, 528, 565, 583, 591, 594, 597, 617
FokI GGATG 1 cut(s) 707
Fsp4HI GCNGC 9 cut(s) 326, 436, 528, 565, 583, 591, 594, 597, 617
FspBI CTAG 1 cut(s) 51
FspI TGCGCA 1 cut(s) 444
GlaI GCGC 3 cut(s) 152, 444, 546
GluI GCNGC 9 cut(s) 326, 436, 528, 565, 583, 591, 594, 597, 617
GsuI CTGGAG 1 cut(s) 172
HaeII RGCGCY 1 cut(s) 154
HaeIII GGCC 3 cut(s) 390, 616, 671
HapII CCGG 6 cut(s) 63, 157, 200, 232, 245, 749
HgaI GACGC 4 cut(s) 47, 519, 596, 781
HhaI GCGC 3 cut(s) 153, 445, 547
Hin1I GRCGYC 2 cut(s) 39, 116
Hin1II CATG 5 cut(s) 166, 385, 670, 759, 850
Hin6I GCGC 3 cut(s) 151, 443, 545
HinP1I GCGC 3 cut(s) 151, 443, 545
HincII GTYRAC 1 cut(s) 311
HindII GTYRAC 1 cut(s) 311
HinfI GANTC 6 cut(s) 94, 142, 185, 254, 854, 860
HpaII CCGG 6 cut(s) 63, 157, 200, 232, 245, 749
HphI GGTGA 2 cut(s) 479, 485
Hpy166II GTNNAC 2 cut(s) 226, 311
Hpy188I TCNGA 9 cut(s) 121, 356, 364, 538, 696, 771, 794, 859, 865
Hpy188III TCNNGA 3 cut(s) 51, 98, 628
Hpy8I GTNNAC 2 cut(s) 226, 311
Hpy99I CGWCG 6 cut(s) 41, 112, 115, 118, 121, 590
HpyAV CCTTC 3 cut(s) 553, 646, 826
HpyCH4III ACNGT 3 cut(s) 217, 318, 783
HpyCH4IV ACGT 1 cut(s) 116
HpyCH4V TGCA 4 cut(s) 133, 162, 377, 755
HpyF10VI GCNNNNNNNGC 4 cut(s) 159, 387, 561, 596
HpyF3I CTNAG 3 cut(s) 342, 353, 693
HpySE526I ACGT 1 cut(s) 116
Hsp92I GRCGYC 2 cut(s) 39, 116
Hsp92II CATG 5 cut(s) 166, 385, 670, 759, 850
HspAI GCGC 3 cut(s) 151, 443, 545
Kzo9I GATC 6 cut(s) 89, 168, 680, 717, 766, 804
LmnI GCTCC 4 cut(s) 401, 572, 579, 693
Lsp1109I GCAGC 3 cut(s) 514, 605, 608
MaeI CTAG 1 cut(s) 51
MaeII ACGT 1 cut(s) 116
MaeIII GTNAC 2 cut(s) 202, 287
MalI GATC 6 cut(s) 91, 170, 682, 719, 768, 806
MbiI CCGCTC 1 cut(s) 582
MboI GATC 6 cut(s) 89, 168, 680, 717, 766, 804
MboII GAAGA 5 cut(s) 178, 668, 811, 814, 826
MflI RGATCY 3 cut(s) 168, 680, 804
MhlI GDGCHC 1 cut(s) 845
MlsI TGGCCA 1 cut(s) 671
MluCI AATT 2 cut(s) 46, 414
MluNI TGGCCA 1 cut(s) 671
MlyI GAGTC 5 cut(s) 103, 179, 248, 854, 863
MmeI TCCRAC 3 cut(s) 99, 748, 837
Mox20I TGGCCA 1 cut(s) 671
MroXI GAANNNNTTC 1 cut(s) 418
MscI TGGCCA 1 cut(s) 671
MseI TTAA 2 cut(s) 33, 675
MslI CAYNNNNRTG 3 cut(s) 498, 760, 848
Msp20I TGGCCA 1 cut(s) 671
MspA1I CMGCKG 1 cut(s) 527
MspI CCGG 6 cut(s) 63, 157, 200, 232, 245, 749
MspR9I CCNGG 5 cut(s) 173, 200, 233, 749, 750
MvaI CCWGG 1 cut(s) 173
MvnI CGCG 1 cut(s) 590
MwoI GCNNNNNNNGC 4 cut(s) 159, 387, 561, 596
NciI CCSGG 4 cut(s) 200, 233, 749, 750
NcoI CCATGG 1 cut(s) 846
NdeII GATC 6 cut(s) 89, 168, 680, 717, 766, 804
NlaIII CATG 5 cut(s) 166, 385, 670, 759, 850
NlaIV GGNNCC 3 cut(s) 568, 682, 844
NsbI TGCGCA 1 cut(s) 444
OliI CACNNNNGTG 1 cut(s) 848
PcsI WCGNNNNNNNCGW 1 cut(s) 113
PdmI GAANNNNTTC 1 cut(s) 418
PfeI GAWTC 1 cut(s) 142
PflFI GACNNNGTC 1 cut(s) 115
PkrI GCNGC 9 cut(s) 327, 437, 529, 566, 584, 592, 595, 598, 618
PleI GAGTC 5 cut(s) 102, 179, 248, 854, 862
PpsI GAGTC 5 cut(s) 102, 179, 248, 854, 862
Psp6I CCWGG 1 cut(s) 171
PspEI GGTNACC 1 cut(s) 202
PspGI CCWGG 1 cut(s) 171
PspN4I GGNNCC 3 cut(s) 568, 682, 844
PsuI RGATCY 3 cut(s) 168, 680, 804
PsyI GACNNNGTC 1 cut(s) 115
RsaI GTAC 2 cut(s) 460, 785
RsaNI GTAC 2 cut(s) 459, 784
RseI CAYNNNNRTG 3 cut(s) 498, 760, 848
SaqAI TTAA 2 cut(s) 33, 675
SatI GCNGC 9 cut(s) 326, 436, 528, 565, 583, 591, 594, 597, 617
Sau3AI GATC 6 cut(s) 89, 168, 680, 717, 766, 804
SchI GAGTC 5 cut(s) 103, 179, 248, 854, 863
ScrFI CCNGG 5 cut(s) 173, 200, 233, 749, 750
SduI GDGCHC 1 cut(s) 845
SetI ASST 9 cut(s) 77, 119, 177, 306, 343, 406, 460, 690, 792
SmaI CCCGGG 1 cut(s) 750
SmiMI CAYNNNNRTG 3 cut(s) 498, 760, 848
Sse9I AATT 2 cut(s) 46, 414
SsiI CCGC 9 cut(s) 229, 326, 435, 525, 564, 582, 590, 602, 617
SspI AATATT 1 cut(s) 31
SspMI CTAG 1 cut(s) 51
StyD4I CCNGG 5 cut(s) 171, 198, 231, 747, 748
StyI CCWWGG 1 cut(s) 846
TaaI ACNGT 3 cut(s) 217, 318, 783
TaiI ACGT 1 cut(s) 119
TaqI TCGA 2 cut(s) 92, 241
TaqII GACCGA 1 cut(s) 835
TasI AATT 2 cut(s) 46, 414
TauI GCSGC 6 cut(s) 328, 438, 567, 585, 593, 619
TfiI GAWTC 1 cut(s) 142
Tru1I TTAA 2 cut(s) 33, 675
Tru9I TTAA 2 cut(s) 33, 675
TscAI CASTG 3 cut(s) 84, 140, 196
TseI GCWGC 3 cut(s) 527, 593, 596
TspDTI ATGAA 2 cut(s) 179, 655
TspGWI ACGGA 2 cut(s) 296, 414
TspMI CCCGGG 1 cut(s) 748
TspRI CASTG 3 cut(s) 84, 140, 196
Tth111I GACNNNGTC 1 cut(s) 115
XapI RAATTY 1 cut(s) 46
XbaI TCTAGA 1 cut(s) 50
XmaI CCCGGG 1 cut(s) 748
XmiI GTMKAC 1 cut(s) 225
XmnI GAANNNNTTC 1 cut(s) 418
XspI CTAG 1 cut(s) 51
ZraI GACGTC 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.