Rw6G043020

Protein of unknown function (DUF1639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0015556
Physical Location & Seq
Reverse (-)
66435394 .. 66440603
5210 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G043020.1

Sequence Viewer

Length: 747 bp
ATGGAAAGAGACTATGTGAGGCACGCCAATATTAACGACACCAAGAATTCTAGAGACAGAACCGGAGGGGATAGCTCACTGCTGTTGAGATCGAGTCCTGATAAAACGACGACGTCCGAGTTCGTTTTGCAGTGGGGGAATCGCAAGCGCCTCCGGTGCATGAAGATCCAGGTCAAGGACGACTCCAGTGGTGTCCCGGTTACCAGAACTACGGTTCGGGTAGACCGCCGGGTCATTCGACCGGACAAGGACTCGTCCAATCAGCCCTCTACCACCAATAATAGCCAGAGCAACGGAGCTATGAGGGGAATTATTTCGCCTGACAGGGGCGGCGTTGCGCACGATAAGAAAGGTACAACTAACAATCACAATCATCACCATCACCATAATGACAATCATCACAACCACAAGTCCGCTGCGTCGTCGGAGACGGCGCACGATAGCAAGAAGGGCGGCTCCTCATCGGGGAGCGGCGACGCGGCAGCAGCACCGCCGATGGTGTGGCCGCCCAAGTTCGTGATTGCCTTGACCAACAAAGAGAAGGAAGAGGATTTCATGGCCATTAAGGGATCCAAGCTCCCTCAGAGACCCAAAAAGAGAGCAAAGATCATCCAACGCACCCTCAATTTTGTACTCTGTCCGGAGCGGACGACATCTAAGATTTCATATCACAAGCCAAAGGCGTTAGTTCTCGAAACAAACTCGTGCACGATATTAAGAAAGAACATAATCAATTCAAATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1639

Protein Analysis

248

Amino Acids

27.63

Weight (kDa)

10.1

Isoelectric Point (pI)

50.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1639 PF07797 176 - 211 2e-15 Protein of unknown function (DUF1639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015556)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g32200
malus_domestica MD08G1073700.v1.1
prunus_persica Prupe.1G415100_v2.0.a1
pyrus_communis pycom08g06100 pycom15g05800
rosa_chinensis RchiOBHm_Chr6g0310471
rosa_laevigata RLG00000010480 RLG00000018734
rosa_multiflora Rmu_sc0020729.1_g000006
rosa_rugosa Rorug06G0380600
rosa_samantha Rh4DG040600 Rh6AG494600 Rh6BG504300 Rh6CG509500 Rh6DG495500
rosa_wichuraiana Rw6G043020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 230
AatII GACGTC 1 cut(s) 116
Acc16I TGCGCA 1 cut(s) 339
AccBSI CCGCTC 2 cut(s) 471, 646
AccI GTMKAC 1 cut(s) 222
AccII CGCG 1 cut(s) 479
AccIII TCCGGA 1 cut(s) 640
AciI CCGC 9 cut(s) 226, 330, 414, 453, 471, 479, 491, 506, 646
AclWI GGATC 3 cut(s) 160, 564, 577
AcoI YGGCCR 2 cut(s) 503, 558
AcsI RAATTY 1 cut(s) 46
AcyI GRCGYC 1 cut(s) 113
AfaI GTAC 2 cut(s) 355, 633
AfiI CCNNNNNNNGG 3 cut(s) 175, 326, 465
AgsI TTSAA 1 cut(s) 738
AjnI CCWGG 1 cut(s) 168
AluBI AGCT 3 cut(s) 75, 299, 577
AluI AGCT 3 cut(s) 75, 299, 577
Alw21I GWGCWC 1 cut(s) 710
Alw26I GTCTC 4 cut(s) 3, 48, 422, 580
Alw44I GTGCAC 1 cut(s) 706
AlwI GGATC 3 cut(s) 160, 564, 577
Aor13HI TCCGGA 1 cut(s) 640
AoxI GGCC 2 cut(s) 503, 558
ApaLI GTGCAC 1 cut(s) 706
ApeKI GCWGC 3 cut(s) 416, 482, 485
ApoI RAATTY 1 cut(s) 46
Asp700I GAANNNNTTC 1 cut(s) 313
AspLEI GCGC 3 cut(s) 150, 340, 436
AsuC2I CCSGG 2 cut(s) 197, 230
AsuHPI GGTGA 2 cut(s) 368, 374
BaeGI GKGCMC 1 cut(s) 710
BalI TGGCCA 1 cut(s) 560
BamHI GGATCC 1 cut(s) 569
BauI CACGAG 1 cut(s) 703
Bbv12I GWGCWC 1 cut(s) 710
BbvI GCAGC 3 cut(s) 403, 494, 497
BccI CCATC 2 cut(s) 387, 490
BceAI ACGGC 1 cut(s) 447
BciT130I CCWGG 1 cut(s) 170
BcnI CCSGG 2 cut(s) 197, 230
BcoDI GTCTC 4 cut(s) 3, 48, 422, 580
BfaI CTAG 1 cut(s) 51
BfoI RGCGCY 1 cut(s) 151
BisI GCNGC 8 cut(s) 331, 417, 454, 472, 480, 483, 486, 506
BlsI GCNGC 8 cut(s) 332, 418, 455, 473, 481, 484, 487, 507
Bme1390I CCNGG 3 cut(s) 170, 197, 230
BmiI GGNNCC 2 cut(s) 457, 571
BmrFI CCNGG 3 cut(s) 170, 197, 230
BpmI CTGGAG 1 cut(s) 169
BpuMI CCSGG 2 cut(s) 197, 230
BsaHI GRCGYC 1 cut(s) 113
BsaI GGTCTC 1 cut(s) 580
BsaWI WCCGGW 4 cut(s) 62, 153, 241, 640
Bsc4I CCNNNNNNNGG 3 cut(s) 175, 326, 465
Bse1I ACTGG 1 cut(s) 186
BseAI TCCGGA 1 cut(s) 640
BseBI CCWGG 1 cut(s) 170
BseGI GGATG 1 cut(s) 609
BseLI CCNNNNNNNGG 3 cut(s) 175, 326, 465
BseMII CTCAG 1 cut(s) 596
BseNI ACTGG 1 cut(s) 186
BseRI GAGGAG 1 cut(s) 448
BseSI GKGCMC 1 cut(s) 710
BseXI GCAGC 3 cut(s) 403, 494, 497
Bsh1236I CGCG 1 cut(s) 479
Bsh1285I CGRYCG 1 cut(s) 242
BshFI GGCC 2 cut(s) 505, 560
BsiEI CGRYCG 1 cut(s) 242
BsiHKAI GWGCWC 1 cut(s) 710
BsiSI CCGG 6 cut(s) 63, 154, 197, 229, 242, 641
BslFI GGGAC 1 cut(s) 179
BslI CCNNNNNNNGG 3 cut(s) 175, 326, 465
BsmAI GTCTC 4 cut(s) 3, 48, 422, 580
BsmBI CGTCTC 1 cut(s) 422
BsmFI GGGAC 1 cut(s) 179
BsnI GGCC 2 cut(s) 505, 560
Bso31I GGTCTC 1 cut(s) 580
Bsp1286I GDGCHC 1 cut(s) 710
Bsp13I TCCGGA 1 cut(s) 640
Bsp143I GATC 4 cut(s) 89, 165, 569, 606
BspACI CCGC 9 cut(s) 226, 330, 414, 453, 471, 479, 491, 506, 646
BspANI GGCC 2 cut(s) 505, 560
BspCNI CTCAG 1 cut(s) 595
BspEI TCCGGA 1 cut(s) 640
BspFNI CGCG 1 cut(s) 479
BspLI GGNNCC 2 cut(s) 457, 571
BspPI GGATC 3 cut(s) 160, 564, 577
BspTNI GGTCTC 1 cut(s) 580
BsrBI CCGCTC 2 cut(s) 471, 646
BsrI ACTGG 1 cut(s) 186
BssMI GATC 4 cut(s) 89, 165, 569, 606
BssNI GRCGYC 1 cut(s) 113
BssSI CACGAG 1 cut(s) 703
Bst2BI CACGAG 1 cut(s) 703
Bst2UI CCWGG 1 cut(s) 170
Bst4CI ACNGT 1 cut(s) 214
Bst6I CTCTTC 1 cut(s) 540
BstACI GRCGYC 1 cut(s) 113
BstC8I GCNNGC 2 cut(s) 24, 146
BstDEI CTNAG 2 cut(s) 582, 657
BstEII GGTNACC 1 cut(s) 199
BstF5I GGATG 1 cut(s) 609
BstFNI CGCG 1 cut(s) 479
BstH2I RGCGCY 1 cut(s) 151
BstHHI GCGC 3 cut(s) 150, 340, 436
BstKTI GATC 4 cut(s) 92, 168, 572, 609
BstMAI GTCTC 4 cut(s) 3, 48, 422, 580
BstMBI GATC 4 cut(s) 89, 165, 569, 606
BstMCI CGRYCG 1 cut(s) 242
BstMWI GCNNNNNNNGC 3 cut(s) 156, 450, 485
BstNI CCWGG 1 cut(s) 170
BstPI GGTNACC 1 cut(s) 199
BstSCI CCNGG 3 cut(s) 168, 195, 228
BstSLI GKGCMC 1 cut(s) 710
BstUI CGCG 1 cut(s) 479
BstV1I GCAGC 3 cut(s) 403, 494, 497
BstX2I RGATCY 2 cut(s) 165, 569
BstYI RGATCY 2 cut(s) 165, 569
BsuRI GGCC 2 cut(s) 505, 560
BtsCI GGATG 1 cut(s) 609
BtsI GCAGTG 2 cut(s) 77, 137
BtsIMutI CAGTG 3 cut(s) 77, 137, 193
Cac8I GCNNGC 2 cut(s) 24, 146
CfoI GCGC 3 cut(s) 150, 340, 436
CseI GACGC 2 cut(s) 408, 485
Csp6I GTAC 2 cut(s) 354, 632
CviAII CATG 2 cut(s) 160, 556
CviJI RGCY 9 cut(s) 75, 265, 285, 299, 456, 505, 560, 577, 676
CviKI_1 RGCY 9 cut(s) 75, 265, 285, 299, 456, 505, 560, 577, 676
CviQI GTAC 2 cut(s) 354, 632
DdeI CTNAG 2 cut(s) 582, 657
DpnI GATC 4 cut(s) 91, 167, 571, 608
DpnII GATC 4 cut(s) 89, 165, 569, 606
DrdI GACNNNNNNGTC 1 cut(s) 230
DseDI GACNNNNNNGTC 1 cut(s) 230
EaeI YGGCCR 2 cut(s) 503, 558
Eam1104I CTCTTC 1 cut(s) 540
EarI CTCTTC 1 cut(s) 540
Eco31I GGTCTC 1 cut(s) 580
Eco91I GGTNACC 1 cut(s) 199
EcoO65I GGTNACC 1 cut(s) 199
EcoRI GAATTC 1 cut(s) 46
EcoRII CCWGG 1 cut(s) 168
Esp3I CGTCTC 1 cut(s) 422
FaeI CATG 2 cut(s) 163, 559
FaiI YATR 7 cut(s) 15, 161, 302, 387, 557, 667, 728
FaqI GGGAC 1 cut(s) 179
FatI CATG 2 cut(s) 159, 555
FblI GTMKAC 1 cut(s) 222
Fnu4HI GCNGC 8 cut(s) 331, 417, 454, 472, 480, 483, 486, 506
FokI GGATG 1 cut(s) 596
Fsp4HI GCNGC 8 cut(s) 331, 417, 454, 472, 480, 483, 486, 506
FspBI CTAG 1 cut(s) 51
FspI TGCGCA 1 cut(s) 339
GlaI GCGC 3 cut(s) 149, 339, 435
GluI GCNGC 8 cut(s) 331, 417, 454, 472, 480, 483, 486, 506
GsuI CTGGAG 1 cut(s) 169
HaeII RGCGCY 1 cut(s) 151
HaeIII GGCC 2 cut(s) 505, 560
HapII CCGG 6 cut(s) 63, 154, 197, 229, 242, 641
HgaI GACGC 2 cut(s) 408, 485
HhaI GCGC 3 cut(s) 150, 340, 436
Hin1I GRCGYC 1 cut(s) 113
Hin1II CATG 2 cut(s) 163, 559
Hin6I GCGC 3 cut(s) 148, 338, 434
HinP1I GCGC 3 cut(s) 148, 338, 434
HinfI GANTC 4 cut(s) 94, 139, 182, 251
HpaII CCGG 6 cut(s) 63, 154, 197, 229, 242, 641
HphI GGTGA 2 cut(s) 368, 374
Hpy166II GTNNAC 2 cut(s) 223, 708
Hpy188I TCNGA 3 cut(s) 118, 427, 585
Hpy188III TCNNGA 5 cut(s) 51, 98, 517, 641, 692
Hpy8I GTNNAC 2 cut(s) 223, 708
Hpy99I CGWCG 5 cut(s) 112, 115, 424, 427, 479
HpyAV CCTTC 2 cut(s) 442, 535
HpyCH4III ACNGT 1 cut(s) 214
HpyCH4IV ACGT 1 cut(s) 113
HpyCH4V TGCA 3 cut(s) 130, 159, 708
HpyF10VI GCNNNNNNNGC 3 cut(s) 156, 450, 485
HpyF3I CTNAG 2 cut(s) 582, 657
HpySE526I ACGT 1 cut(s) 113
Hsp92I GRCGYC 1 cut(s) 113
Hsp92II CATG 2 cut(s) 163, 559
HspAI GCGC 3 cut(s) 148, 338, 434
Kpn2I TCCGGA 1 cut(s) 640
Kzo9I GATC 4 cut(s) 89, 165, 569, 606
LmnI GCTCC 5 cut(s) 296, 461, 468, 582, 643
Lsp1109I GCAGC 3 cut(s) 403, 494, 497
MaeI CTAG 1 cut(s) 51
MaeII ACGT 1 cut(s) 113
MaeIII GTNAC 1 cut(s) 199
MalI GATC 4 cut(s) 91, 167, 571, 608
MbiI CCGCTC 2 cut(s) 471, 646
MboI GATC 4 cut(s) 89, 165, 569, 606
MboII GAAGA 2 cut(s) 175, 557
MflI RGATCY 2 cut(s) 165, 569
MhlI GDGCHC 1 cut(s) 710
MlsI TGGCCA 1 cut(s) 560
MluCI AATT 4 cut(s) 46, 309, 625, 733
MluNI TGGCCA 1 cut(s) 560
MlyI GAGTC 3 cut(s) 103, 176, 245
MmeI TCCRAC 2 cut(s) 405, 637
MnlI CCTC 9 cut(s) 12, 59, 161, 277, 297, 469, 541, 591, 632
Mox20I TGGCCA 1 cut(s) 560
MroI TCCGGA 1 cut(s) 640
MroXI GAANNNNTTC 1 cut(s) 313
MscI TGGCCA 1 cut(s) 560
MseI TTAA 3 cut(s) 33, 564, 716
MslI CAYNNNNRTG 1 cut(s) 387
Msp20I TGGCCA 1 cut(s) 560
MspA1I CMGCKG 1 cut(s) 416
MspI CCGG 6 cut(s) 63, 154, 197, 229, 242, 641
MspR9I CCNGG 3 cut(s) 170, 197, 230
MvaI CCWGG 1 cut(s) 170
MvnI CGCG 1 cut(s) 479
MwoI GCNNNNNNNGC 3 cut(s) 156, 450, 485
NciI CCSGG 2 cut(s) 197, 230
NdeII GATC 4 cut(s) 89, 165, 569, 606
NlaIII CATG 2 cut(s) 163, 559
NlaIV GGNNCC 2 cut(s) 457, 571
NsbI TGCGCA 1 cut(s) 339
PdmI GAANNNNTTC 1 cut(s) 313
PfeI GAWTC 1 cut(s) 139
PflFI GACNNNGTC 1 cut(s) 112
PkrI GCNGC 8 cut(s) 332, 418, 455, 473, 481, 484, 487, 507
PleI GAGTC 3 cut(s) 102, 176, 245
PpsI GAGTC 3 cut(s) 102, 176, 245
Psp6I CCWGG 1 cut(s) 168
PspEI GGTNACC 1 cut(s) 199
PspGI CCWGG 1 cut(s) 168
PspN4I GGNNCC 2 cut(s) 457, 571
PsuI RGATCY 2 cut(s) 165, 569
PsyI GACNNNGTC 1 cut(s) 112
RsaI GTAC 2 cut(s) 355, 633
RsaNI GTAC 2 cut(s) 354, 632
RseI CAYNNNNRTG 1 cut(s) 387
SaqAI TTAA 3 cut(s) 33, 564, 716
SatI GCNGC 8 cut(s) 331, 417, 454, 472, 480, 483, 486, 506
Sau3AI GATC 4 cut(s) 89, 165, 569, 606
SchI GAGTC 3 cut(s) 103, 176, 245
ScrFI CCNGG 3 cut(s) 170, 197, 230
SduI GDGCHC 1 cut(s) 710
SetI ASST 6 cut(s) 77, 116, 174, 301, 355, 579
SmiMI CAYNNNNRTG 1 cut(s) 387
Sse9I AATT 4 cut(s) 46, 309, 625, 733
SsiI CCGC 9 cut(s) 226, 330, 414, 453, 471, 479, 491, 506, 646
SspI AATATT 1 cut(s) 31
SspMI CTAG 1 cut(s) 51
StyD4I CCNGG 3 cut(s) 168, 195, 228
TaaI ACNGT 1 cut(s) 214
TaiI ACGT 1 cut(s) 116
TaqI TCGA 3 cut(s) 92, 238, 693
TasI AATT 4 cut(s) 46, 309, 625, 733
TatI WGTACW 1 cut(s) 631
TauI GCSGC 5 cut(s) 333, 456, 474, 482, 508
TfiI GAWTC 1 cut(s) 139
Tru1I TTAA 3 cut(s) 33, 564, 716
Tru9I TTAA 3 cut(s) 33, 564, 716
TscAI CASTG 3 cut(s) 84, 137, 193
TseI GCWGC 3 cut(s) 416, 482, 485
TspDTI ATGAA 3 cut(s) 176, 544, 654
TspGWI ACGGA 1 cut(s) 309
TspRI CASTG 3 cut(s) 84, 137, 193
Tth111I GACNNNGTC 1 cut(s) 112
VneI GTGCAC 1 cut(s) 706
XapI RAATTY 1 cut(s) 46
XbaI TCTAGA 1 cut(s) 50
XmiI GTMKAC 1 cut(s) 222
XmnI GAANNNNTTC 1 cut(s) 313
XspI CTAG 1 cut(s) 51
ZraI GACGTC 1 cut(s) 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.