FvH4_2g33950

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010893
Physical Location & Seq
Forward (+)
25321850 .. 25323878
2029 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g33950.t1

Sequence Viewer

Length: 537 bp
ATGGAGCGTACACACACAGAGATGATGGAGACGGAGATGGAGGTCGACACCGCCGCTACTGGGCGGCCGGAGCAGTCGTCTAATACCGGCCAAAGTGACGTCGTCAGGGACTTGCTCACATTGGCTCGCCAGCTCGTCGGTGAAGGAAAGCCTACTCAGGCCCTGCAGGCGGTTGTTATGGCAGCAAGAACCAGAGGTGGGGATGAGGCCGTTGTTCAGTCCTTGCAACGTGCTCGTGAGCTATACAGATGTAGATTGCAAGAAAGCACTGCTGTTGATCAGTTAGCCTCTTTGTTTGCTGAGTGTGCAATTGCTGAAGCTCAGCCTTCACAACCTGAATCGTCAGCAGAGAACATCAGTGGCCCATCAGTTGGACCGGATGCTCAAGGAACTTCTATACTGGCGGAAACTGGCAGGATGCAGATTGTGCTGGATGCATTTTCAGATGGGAGCAGCTTCATTTGCTTACAGTGTGGAGGTCTAGTTAGCAATCATCGGAAAGACGAGCACTATACCTACTGGTGTTGCCAAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

179

Amino Acids

19.28

Weight (kDa)

4.64

Isoelectric Point (pI)

42.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C2HC_3 PF25017 131 - 176 3.5e-26 C2HC zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010893)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G47830 AT5G47830
fragaria_vesca FvH4_2g33950
malus_domestica MD06G1210300.v1.1 MD08G1095400.v1.1 MD15G1077900.v1.1 MD17G1062600.v1.1
prunus_persica Prupe.1G431400_v2.0.a1
pyrus_communis pycom15g07260
rosa_chinensis RchiOBHm_Chr6g0308461
rosa_laevigata RLG00000010641
rosa_multiflora Rmu_co8427491.1_g000001 Rmu_sc0011796.1_g000007
rosa_roxburghii Rroxscaffold_7G00160110
rosa_rugosa Rorug06G0367000
rosa_samantha Rh6AG476700 Rh6BG486900 Rh6CG492000 Rh6DG478100
rosa_wichuraiana Rw6G041580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 102
AccB7I CCANNNNNTGG 1 cut(s) 371
AccI GTMKAC 1 cut(s) 45
AciI CCGC 5 cut(s) 51, 54, 64, 170, 404
AcoI YGGCCR 2 cut(s) 65, 88
AcsI RAATTY 1 cut(s) 531
AcuI CTGAAG 1 cut(s) 336
AcyI GRCGYC 1 cut(s) 99
AfaI GTAC 1 cut(s) 10
AfiI CCNNNNNNNGG 4 cut(s) 60, 169, 198, 371
AluBI AGCT 4 cut(s) 133, 241, 320, 456
AluI AGCT 4 cut(s) 133, 241, 320, 456
Alw21I GWGCWC 2 cut(s) 235, 510
Alw26I GTCTC 1 cut(s) 23
AlwNI CAGNNNCTG 1 cut(s) 163
AoxI GGCC 5 cut(s) 65, 88, 159, 207, 361
ApeKI GCWGC 2 cut(s) 182, 453
ApoI RAATTY 1 cut(s) 531
AspS9I GGNCC 3 cut(s) 160, 362, 374
AsuHPI GGTGA 1 cut(s) 152
AvaII GGWCC 1 cut(s) 374
BauI CACGAG 1 cut(s) 234
Bbv12I GWGCWC 2 cut(s) 235, 510
BbvI GCAGC 2 cut(s) 194, 465
BccI CCATC 4 cut(s) 19, 31, 373, 440
BceAI ACGGC 1 cut(s) 194
BcgI CGANNNNNNTGC 2 cut(s) 215, 249
BclI TGATCA 1 cut(s) 277
BcoDI GTCTC 1 cut(s) 23
BfaI CTAG 1 cut(s) 482
BfmI CTRYAG 1 cut(s) 164
BglI GCCNNNNNGGC 1 cut(s) 167
BisI GCNGC 4 cut(s) 54, 65, 183, 454
BlpI GCTNAGC 1 cut(s) 321
BlsI GCNGC 4 cut(s) 55, 66, 184, 455
Bme18I GGWCC 1 cut(s) 374
BmgT120I GGNCC 3 cut(s) 160, 362, 374
BmrI ACTGGG 1 cut(s) 69
BmsI GCATC 3 cut(s) 370, 408, 424
BmuI ACTGGG 1 cut(s) 69
Bpu1102I GCTNAGC 1 cut(s) 321
BpuEI CTTGAG 1 cut(s) 369
BsaHI GRCGYC 1 cut(s) 99
BsaWI WCCGGW 1 cut(s) 376
BsaXI ACNNNNNCTCC 4 cut(s) 62, 92, 468, 498
Bsc4I CCNNNNNNNGG 4 cut(s) 60, 169, 198, 371
Bse118I RCCGGY 1 cut(s) 86
Bse1I ACTGG 4 cut(s) 64, 405, 415, 524
BseGI GGATG 4 cut(s) 208, 385, 423, 439
BseLI CCNNNNNNNGG 4 cut(s) 60, 169, 198, 371
BseMII CTCAG 3 cut(s) 170, 291, 335
BseNI ACTGG 4 cut(s) 64, 405, 415, 524
BseX3I CGGCCG 1 cut(s) 65
BseXI GCAGC 2 cut(s) 194, 465
Bsh1285I CGRYCG 1 cut(s) 68
BshFI GGCC 5 cut(s) 67, 90, 161, 209, 363
BsiEI CGRYCG 1 cut(s) 68
BsiHKAI GWGCWC 2 cut(s) 235, 510
BsiSI CCGG 3 cut(s) 68, 87, 377
BslFI GGGAC 1 cut(s) 122
BslI CCNNNNNNNGG 4 cut(s) 60, 169, 198, 371
BsmAI GTCTC 1 cut(s) 23
BsmBI CGTCTC 1 cut(s) 23
BsmFI GGGAC 1 cut(s) 122
BsnI GGCC 5 cut(s) 67, 90, 161, 209, 363
Bsp1286I GDGCHC 2 cut(s) 235, 510
Bsp143I GATC 1 cut(s) 277
Bsp1720I GCTNAGC 1 cut(s) 321
BspACI CCGC 5 cut(s) 51, 54, 64, 170, 404
BspANI GGCC 5 cut(s) 67, 90, 161, 209, 363
BspCNI CTCAG 3 cut(s) 169, 292, 334
BspMAI CTGCAG 1 cut(s) 168
BsrFI RCCGGY 1 cut(s) 86
BsrI ACTGG 4 cut(s) 64, 405, 415, 524
BssAI RCCGGY 1 cut(s) 86
BssMI GATC 1 cut(s) 277
BssNI GRCGYC 1 cut(s) 99
BssSI CACGAG 1 cut(s) 234
Bst2BI CACGAG 1 cut(s) 234
Bst4CI ACNGT 1 cut(s) 471
BstACI GRCGYC 1 cut(s) 99
BstAPI GCANNNNNTGC 1 cut(s) 427
BstC8I GCNNGC 3 cut(s) 127, 131, 168
BstDEI CTNAG 3 cut(s) 156, 300, 321
BstF5I GGATG 4 cut(s) 208, 385, 423, 439
BstKTI GATC 1 cut(s) 280
BstMAI GTCTC 1 cut(s) 23
BstMBI GATC 1 cut(s) 277
BstMCI CGRYCG 1 cut(s) 68
BstMWI GCNNNNNNNGC 5 cut(s) 70, 167, 305, 427, 462
BstSFI CTRYAG 1 cut(s) 164
BstV1I GCAGC 2 cut(s) 194, 465
BstZI CGGCCG 1 cut(s) 65
BsuRI GGCC 5 cut(s) 67, 90, 161, 209, 363
BtsCI GGATG 4 cut(s) 208, 385, 423, 439
BtsI GCAGTG 1 cut(s) 267
BtsIMutI CAGTG 3 cut(s) 267, 364, 476
Cac8I GCNNGC 3 cut(s) 127, 131, 168
CaiI CAGNNNCTG 1 cut(s) 163
Cfr10I RCCGGY 1 cut(s) 86
Cfr13I GGNCC 3 cut(s) 160, 362, 374
Csp6I GTAC 1 cut(s) 9
CviQI GTAC 1 cut(s) 9
DdeI CTNAG 3 cut(s) 156, 300, 321
DpnI GATC 1 cut(s) 279
DpnII GATC 1 cut(s) 277
EaeI YGGCCR 2 cut(s) 65, 88
EagI CGGCCG 1 cut(s) 65
EciI GGCGGA 1 cut(s) 419
EclXI CGGCCG 1 cut(s) 65
Eco47I GGWCC 1 cut(s) 374
Eco52I CGGCCG 1 cut(s) 65
Eco57I CTGAAG 1 cut(s) 336
EcoO109I RGGNCCY 1 cut(s) 160
EcoT22I ATGCAT 1 cut(s) 439
Esp3I CGTCTC 1 cut(s) 23
FaiI YATR 4 cut(s) 179, 244, 398, 513
FaqI GGGAC 1 cut(s) 122
FbaI TGATCA 1 cut(s) 277
FblI GTMKAC 1 cut(s) 45
Fnu4HI GCNGC 4 cut(s) 54, 65, 183, 454
FokI GGATG 4 cut(s) 215, 392, 430, 446
Fsp4HI GCNGC 4 cut(s) 54, 65, 183, 454
FspBI CTAG 1 cut(s) 482
GluI GCNGC 4 cut(s) 54, 65, 183, 454
HaeIII GGCC 5 cut(s) 67, 90, 161, 209, 363
HapII CCGG 3 cut(s) 68, 87, 377
Hin1I GRCGYC 1 cut(s) 99
HincII GTYRAC 1 cut(s) 46
HindII GTYRAC 1 cut(s) 46
HinfI GANTC 1 cut(s) 338
HpaII CCGG 3 cut(s) 68, 87, 377
HphI GGTGA 1 cut(s) 152
Hpy166II GTNNAC 2 cut(s) 11, 46
Hpy188I TCNGA 2 cut(s) 445, 498
Hpy188III TCNNGA 1 cut(s) 236
Hpy8I GTNNAC 2 cut(s) 11, 46
Hpy99I CGWCG 2 cut(s) 104, 140
HpyAV CCTTC 2 cut(s) 137, 336
HpyCH4III ACNGT 1 cut(s) 471
HpyCH4IV ACGT 2 cut(s) 99, 229
HpyCH4V TGCA 6 cut(s) 166, 226, 259, 308, 421, 437
HpyF10VI GCNNNNNNNGC 5 cut(s) 70, 167, 305, 427, 462
HpyF3I CTNAG 3 cut(s) 156, 300, 321
HpySE526I ACGT 2 cut(s) 99, 229
Hsp92I GRCGYC 1 cut(s) 99
Ksp22I TGATCA 1 cut(s) 277
Kzo9I GATC 1 cut(s) 277
LmnI GCTCC 3 cut(s) 4, 70, 450
Lsp1109I GCAGC 2 cut(s) 194, 465
LweI GCATC 3 cut(s) 370, 408, 424
MaeI CTAG 1 cut(s) 482
MaeII ACGT 2 cut(s) 99, 229
MaeIII GTNAC 1 cut(s) 95
MalI GATC 1 cut(s) 279
MboI GATC 1 cut(s) 277
MfeI CAATTG 1 cut(s) 309
MhlI GDGCHC 2 cut(s) 235, 510
MluCI AATT 2 cut(s) 309, 531
MmeI TCCRAC 1 cut(s) 352
MnlI CCTC 5 cut(s) 34, 188, 199, 298, 470
Mph1103I ATGCAT 1 cut(s) 439
MslI CAYNNNNRTG 1 cut(s) 20
MspI CCGG 3 cut(s) 68, 87, 377
MunI CAATTG 1 cut(s) 309
MwoI GCNNNNNNNGC 5 cut(s) 70, 167, 305, 427, 462
NdeII GATC 1 cut(s) 277
NmuCI GTSAC 1 cut(s) 95
NsiI ATGCAT 1 cut(s) 439
PfeI GAWTC 1 cut(s) 338
PflFI GACNNNGTC 1 cut(s) 101
PflMI CCANNNNNTGG 1 cut(s) 371
PkrI GCNGC 4 cut(s) 55, 66, 184, 455
PspPI GGNCC 3 cut(s) 160, 362, 374
PstI CTGCAG 1 cut(s) 168
PstNI CAGNNNCTG 1 cut(s) 163
PsyI GACNNNGTC 1 cut(s) 101
RsaI GTAC 1 cut(s) 10
RsaNI GTAC 1 cut(s) 9
RseI CAYNNNNRTG 1 cut(s) 20
SalI GTCGAC 1 cut(s) 44
SatI GCNGC 4 cut(s) 54, 65, 183, 454
Sau3AI GATC 1 cut(s) 277
Sau96I GGNCC 3 cut(s) 160, 362, 374
SbfI CCTGCAGG 1 cut(s) 168
SdaI CCTGCAGG 1 cut(s) 168
SduI GDGCHC 2 cut(s) 235, 510
SfaNI GCATC 3 cut(s) 370, 408, 424
SfcI CTRYAG 1 cut(s) 164
SinI GGWCC 1 cut(s) 374
SmiMI CAYNNNNRTG 1 cut(s) 20
SmlI CTYRAG 1 cut(s) 384
SmoI CTYRAG 1 cut(s) 384
Sse8387I CCTGCAGG 1 cut(s) 168
Sse9I AATT 2 cut(s) 309, 531
SsiI CCGC 5 cut(s) 51, 54, 64, 170, 404
SspMI CTAG 1 cut(s) 482
TaaI ACNGT 1 cut(s) 471
TaiI ACGT 2 cut(s) 102, 232
TaqI TCGA 1 cut(s) 45
TasI AATT 2 cut(s) 309, 531
TauI GCSGC 2 cut(s) 56, 67
TfiI GAWTC 1 cut(s) 338
TscAI CASTG 3 cut(s) 274, 364, 476
TseFI GTSAC 1 cut(s) 95
TseI GCWGC 2 cut(s) 182, 453
Tsp45I GTSAC 1 cut(s) 95
TspDTI ATGAA 1 cut(s) 448
TspGWI ACGGA 1 cut(s) 47
TspRI CASTG 3 cut(s) 274, 364, 476
Tth111I GACNNNGTC 1 cut(s) 101
Van91I CCANNNNNTGG 1 cut(s) 371
VpaK11BI GGWCC 1 cut(s) 374
XapI RAATTY 1 cut(s) 531
XmiI GTMKAC 1 cut(s) 45
XspI CTAG 1 cut(s) 482
ZraI GACGTC 1 cut(s) 100
Zsp2I ATGCAT 1 cut(s) 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.