MD17G1062600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0010893
Physical Location & Seq
Reverse (-)
5080768 .. 5081282
515 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1062600.v1.1.491

Sequence Viewer

Length: 465 bp
ATGATGGAGGTGGAGACGACGGCGGCTGGCCGGCTGGAGCTGCTGTCCAGGACTGGCCAAGGTGACGTCTCCAGGGACTTGCTCACATTGTCTCGCCAGCTCCTCAATCAAGGCAAGCCTTCCCAGGCCCTCCAAGTGGTTGTTATGGCAATGAGAACAAGAGGTGGGGACGAGGCTGTATTTCAGTCCTTGCATCATGCTCGTGAGCTGTACAGAAGTAGATTGCAAGAAAGCTCTGCTGCTGATCAACTAGCTTCTTTGTTTTCCGAATGTGCAATTGCCGAAGCTCATCCTTGGAAGACTGAACCAGAAGCAGCATATAACGTGGGTGGTCCATCGGTTGGTCTTGGACCTGATGGTCAAGGAAGTTCCATTCTAGCGGAAACTGGCAGGATGCAGATTGTTTTGGATGCATTTTCAGATGGGAGTAGCTTCATTTGTTTACAATGCGAAGGTCTTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

155

Amino Acids

16.51

Weight (kDa)

4.88

Isoelectric Point (pI)

42.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C2HC_3 PF25017 123 - 153 3.6e-14 C2HC zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010893)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G47830 AT5G47830
fragaria_vesca FvH4_2g33950
malus_domestica MD06G1210300.v1.1 MD08G1095400.v1.1 MD15G1077900.v1.1 MD17G1062600.v1.1
prunus_persica Prupe.1G431400_v2.0.a1
pyrus_communis pycom15g07260
rosa_chinensis RchiOBHm_Chr6g0308461
rosa_laevigata RLG00000010641
rosa_multiflora Rmu_co8427491.1_g000001 Rmu_sc0011796.1_g000007
rosa_roxburghii Rroxscaffold_7G00160110
rosa_rugosa Rorug06G0367000
rosa_samantha Rh6AG476700 Rh6BG486900 Rh6CG492000 Rh6DG478100
rosa_wichuraiana Rw6G041580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 357
AatII GACGTC 1 cut(s) 69
AccB7I CCANNNNNTGG 1 cut(s) 341
AciI CCGC 2 cut(s) 23, 380
AcoI YGGCCR 2 cut(s) 28, 55
AcyI GRCGYC 1 cut(s) 66
AfaI GTAC 1 cut(s) 212
AfiI CCNNNNNNNGG 2 cut(s) 136, 341
AgsI TTSAA 1 cut(s) 461
AjnI CCWGG 3 cut(s) 47, 71, 123
AluBI AGCT 7 cut(s) 40, 100, 208, 234, 254, 287, 432
AluI AGCT 7 cut(s) 40, 100, 208, 234, 254, 287, 432
Alw26I GTCTC 3 cut(s) 8, 73, 96
AoxI GGCC 3 cut(s) 28, 55, 126
ApeKI GCWGC 3 cut(s) 40, 239, 314
Asp700I GAANNNNTTC 1 cut(s) 456
AspS9I GGNCC 3 cut(s) 127, 332, 350
AsuHPI GGTGA 1 cut(s) 74
AvaII GGWCC 2 cut(s) 332, 350
BalI TGGCCA 1 cut(s) 57
BauI CACGAG 1 cut(s) 201
BbsI GAAGAC 2 cut(s) 305, 449
BbvI GCAGC 3 cut(s) 27, 226, 326
BccI CCATC 3 cut(s) 343, 350, 416
BceAI ACGGC 1 cut(s) 36
BcgI CGANNNNNNTGC 2 cut(s) 182, 216
BciT130I CCWGG 3 cut(s) 49, 73, 125
BclI TGATCA 1 cut(s) 244
BcoDI GTCTC 3 cut(s) 8, 73, 96
BfaI CTAG 2 cut(s) 251, 377
BisI GCNGC 4 cut(s) 24, 41, 240, 315
BlsI GCNGC 4 cut(s) 25, 42, 241, 316
Bme1390I CCNGG 3 cut(s) 49, 73, 125
Bme18I GGWCC 2 cut(s) 332, 350
BmgT120I GGNCC 3 cut(s) 127, 332, 350
BmrFI CCNGG 3 cut(s) 49, 73, 125
BmsI GCATC 3 cut(s) 202, 384, 400
BpiI GAAGAC 2 cut(s) 305, 449
BpmI CTGGAG 2 cut(s) 55, 56
BsaHI GRCGYC 1 cut(s) 66
BsaJI CCNNGG 4 cut(s) 58, 72, 123, 293
BsaXI ACNNNNNCTCC 2 cut(s) 29, 59
Bsc4I CCNNNNNNNGG 2 cut(s) 136, 341
Bse118I RCCGGY 1 cut(s) 30
Bse1I ACTGG 2 cut(s) 58, 391
Bse3DI GCAATG 1 cut(s) 156
BseBI CCWGG 3 cut(s) 49, 73, 125
BseDI CCNNGG 4 cut(s) 58, 72, 123, 293
BseGI GGATG 3 cut(s) 289, 399, 415
BseLI CCNNNNNNNGG 2 cut(s) 136, 341
BseMI GCAATG 1 cut(s) 156
BseNI ACTGG 2 cut(s) 58, 391
BseRI GAGGAG 1 cut(s) 92
BseXI GCAGC 3 cut(s) 27, 226, 326
BshFI GGCC 3 cut(s) 30, 57, 128
BsiSI CCGG 1 cut(s) 31
BslFI GGGAC 2 cut(s) 89, 182
BslI CCNNNNNNNGG 2 cut(s) 136, 341
BsmAI GTCTC 3 cut(s) 8, 73, 96
BsmBI CGTCTC 2 cut(s) 8, 73
BsmFI GGGAC 2 cut(s) 89, 182
BsnI GGCC 3 cut(s) 30, 57, 128
Bsp1407I TGTACA 1 cut(s) 210
Bsp143I GATC 1 cut(s) 244
BspACI CCGC 2 cut(s) 23, 380
BspANI GGCC 3 cut(s) 30, 57, 128
BsrDI GCAATG 1 cut(s) 156
BsrFI RCCGGY 1 cut(s) 30
BsrGI TGTACA 1 cut(s) 210
BsrI ACTGG 2 cut(s) 58, 391
BssAI RCCGGY 1 cut(s) 30
BssECI CCNNGG 4 cut(s) 58, 72, 123, 293
BssMI GATC 1 cut(s) 244
BssNI GRCGYC 1 cut(s) 66
BssSI CACGAG 1 cut(s) 201
BssT1I CCWWGG 2 cut(s) 58, 293
Bst2BI CACGAG 1 cut(s) 201
Bst2UI CCWGG 3 cut(s) 49, 73, 125
BstACI GRCGYC 1 cut(s) 66
BstAUI TGTACA 1 cut(s) 210
BstC8I GCNNGC 4 cut(s) 28, 32, 98, 116
BstF5I GGATG 3 cut(s) 289, 399, 415
BstKTI GATC 1 cut(s) 247
BstMAI GTCTC 3 cut(s) 8, 73, 96
BstMBI GATC 1 cut(s) 244
BstMWI GCNNNNNNNGC 1 cut(s) 40
BstNI CCWGG 3 cut(s) 49, 73, 125
BstSCI CCNGG 3 cut(s) 47, 71, 123
BstV1I GCAGC 3 cut(s) 27, 226, 326
BstV2I GAAGAC 2 cut(s) 305, 449
BsuRI GGCC 3 cut(s) 30, 57, 128
BtsCI GGATG 3 cut(s) 289, 399, 415
Cac8I GCNNGC 4 cut(s) 28, 32, 98, 116
Cfr10I RCCGGY 1 cut(s) 30
Cfr13I GGNCC 3 cut(s) 127, 332, 350
Csp6I GTAC 1 cut(s) 211
CviAII CATG 1 cut(s) 197
CviQI GTAC 1 cut(s) 211
DpnI GATC 1 cut(s) 246
DpnII GATC 1 cut(s) 244
DrdI GACNNNNNNGTC 1 cut(s) 357
DseDI GACNNNNNNGTC 1 cut(s) 357
EaeI YGGCCR 2 cut(s) 28, 55
Eco130I CCWWGG 2 cut(s) 58, 293
Eco47I GGWCC 2 cut(s) 332, 350
EcoO109I RGGNCCY 1 cut(s) 127
EcoRII CCWGG 3 cut(s) 47, 71, 123
EcoT14I CCWWGG 2 cut(s) 58, 293
EcoT22I ATGCAT 1 cut(s) 415
ErhI CCWWGG 2 cut(s) 58, 293
Esp3I CGTCTC 2 cut(s) 8, 73
FaeI CATG 1 cut(s) 200
FaiI YATR 4 cut(s) 146, 198, 319, 321
FaqI GGGAC 2 cut(s) 89, 182
FatI CATG 1 cut(s) 196
FbaI TGATCA 1 cut(s) 244
Fnu4HI GCNGC 4 cut(s) 24, 41, 240, 315
FokI GGATG 3 cut(s) 276, 406, 422
Fsp4HI GCNGC 4 cut(s) 24, 41, 240, 315
FspBI CTAG 2 cut(s) 251, 377
GluI GCNGC 4 cut(s) 24, 41, 240, 315
GsuI CTGGAG 2 cut(s) 55, 56
HaeIII GGCC 3 cut(s) 30, 57, 128
HapII CCGG 1 cut(s) 31
Hin1I GRCGYC 1 cut(s) 66
Hin1II CATG 1 cut(s) 200
HpaII CCGG 1 cut(s) 31
HphI GGTGA 1 cut(s) 74
Hpy166II GTNNAC 1 cut(s) 443
Hpy188I TCNGA 2 cut(s) 268, 421
Hpy188III TCNNGA 1 cut(s) 203
Hpy8I GTNNAC 1 cut(s) 443
Hpy99I CGWCG 1 cut(s) 22
HpyAV CCTTC 2 cut(s) 129, 446
HpyCH4IV ACGT 2 cut(s) 66, 324
HpyCH4V TGCA 5 cut(s) 193, 226, 275, 397, 413
HpyF10VI GCNNNNNNNGC 1 cut(s) 40
HpySE526I ACGT 2 cut(s) 66, 324
Hsp92I GRCGYC 1 cut(s) 66
Hsp92II CATG 1 cut(s) 200
KroI GCCGGC 1 cut(s) 30
KroNI GCCGGC 1 cut(s) 32
Ksp22I TGATCA 1 cut(s) 244
Kzo9I GATC 1 cut(s) 244
LmnI GCTCC 2 cut(s) 37, 105
Lsp1109I GCAGC 3 cut(s) 27, 226, 326
LweI GCATC 3 cut(s) 202, 384, 400
MaeI CTAG 2 cut(s) 251, 377
MaeII ACGT 2 cut(s) 66, 324
MaeIII GTNAC 1 cut(s) 62
MalI GATC 1 cut(s) 246
MboI GATC 1 cut(s) 244
MboII GAAGA 2 cut(s) 310, 449
MfeI CAATTG 1 cut(s) 276
MlsI TGGCCA 1 cut(s) 57
MluCI AATT 1 cut(s) 276
MluNI TGGCCA 1 cut(s) 57
MnlI CCTC 4 cut(s) 113, 140, 155, 166
Mox20I TGGCCA 1 cut(s) 57
Mph1103I ATGCAT 1 cut(s) 415
MroNI GCCGGC 1 cut(s) 30
MroXI GAANNNNTTC 1 cut(s) 456
MscI TGGCCA 1 cut(s) 57
MslI CAYNNNNRTG 1 cut(s) 201
Msp20I TGGCCA 1 cut(s) 57
MspI CCGG 1 cut(s) 31
MspR9I CCNGG 3 cut(s) 49, 73, 125
MunI CAATTG 1 cut(s) 276
MvaI CCWGG 3 cut(s) 49, 73, 125
MwoI GCNNNNNNNGC 1 cut(s) 40
NaeI GCCGGC 1 cut(s) 32
NdeII GATC 1 cut(s) 244
NgoMIV GCCGGC 1 cut(s) 30
NlaIII CATG 1 cut(s) 200
NmuCI GTSAC 1 cut(s) 62
NsiI ATGCAT 1 cut(s) 415
PdiI GCCGGC 1 cut(s) 32
PdmI GAANNNNTTC 1 cut(s) 456
PflMI CCANNNNNTGG 1 cut(s) 341
PfoI TCCNGGA 1 cut(s) 47
PkrI GCNGC 4 cut(s) 25, 42, 241, 316
Psp6I CCWGG 3 cut(s) 47, 71, 123
PspGI CCWGG 3 cut(s) 47, 71, 123
PspPI GGNCC 3 cut(s) 127, 332, 350
RsaI GTAC 1 cut(s) 212
RsaNI GTAC 1 cut(s) 211
RseI CAYNNNNRTG 1 cut(s) 201
SatI GCNGC 4 cut(s) 24, 41, 240, 315
Sau3AI GATC 1 cut(s) 244
Sau96I GGNCC 3 cut(s) 127, 332, 350
ScrFI CCNGG 3 cut(s) 49, 73, 125
SfaNI GCATC 3 cut(s) 202, 384, 400
SinI GGWCC 2 cut(s) 332, 350
SmiMI CAYNNNNRTG 1 cut(s) 201
Sse9I AATT 1 cut(s) 276
SsiI CCGC 2 cut(s) 23, 380
SspMI CTAG 2 cut(s) 251, 377
StyD4I CCNGG 3 cut(s) 47, 71, 123
StyI CCWWGG 2 cut(s) 58, 293
TaiI ACGT 2 cut(s) 69, 327
TasI AATT 1 cut(s) 276
TatI WGTACW 1 cut(s) 210
TauI GCSGC 1 cut(s) 26
TseFI GTSAC 1 cut(s) 62
TseI GCWGC 3 cut(s) 40, 239, 314
Tsp45I GTSAC 1 cut(s) 62
TspDTI ATGAA 1 cut(s) 424
Van91I CCANNNNNTGG 1 cut(s) 341
VpaK11BI GGWCC 2 cut(s) 332, 350
XmnI GAANNNNTTC 1 cut(s) 456
XspI CTAG 2 cut(s) 251, 377
ZraI GACGTC 1 cut(s) 67
Zsp2I ATGCAT 1 cut(s) 415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.