MD15G1077900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010893
Physical Location & Seq
Reverse (-)
5319134 .. 5320819
1686 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1077900.v1.1.491

Sequence Viewer

Length: 510 bp
ATGAACCATACGGACACGGAGATGATGGAGACGGCGGCGCCGTCCAACACCGGCCAAGGCGACGTCGTCAGGGACTTGCTCACATTGTCTCGCCAGCTCCTTAATCAAGGCAAGCCTTCCCAGGCCCTTCAAGCGGTTGTTATGGCAATGAGAACAAGGGGTGGGGAGGAGGCTGTATTTCAGTCCTTACATCGTGCTCGTGAGCTGTACAGAAGTAGATTGCAAGAAAGCGCTGCTGCTGATCAACTAGCTTCTTTGTTTGCGGAGTGTGCAATTGCCGAAGCTCATCCTTGGAAGACTGAACCAGAAGCAGCAGGTAACGTGGGTGGTCCGTCAGTTGGTCCTGGACCTGATGATCAAGGAAGTTCCATACTGGCGGAAACTGGCAGGATGCAGGTTGTGTTGGATGCATTTTCAGATGGGAGTAGCTTCATTTGTTTACAGTGTGGAGGTCTAGTTAGCAATCACCGCAAAGACGAGCACTACACATACTGGTGTTGCCAAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

170

Amino Acids

18.24

Weight (kDa)

5.01

Isoelectric Point (pI)

37.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C2HC_3 PF25017 122 - 167 4.6e-26 C2HC zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010893)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G47830 AT5G47830
fragaria_vesca FvH4_2g33950
malus_domestica MD06G1210300.v1.1 MD08G1095400.v1.1 MD15G1077900.v1.1 MD17G1062600.v1.1
prunus_persica Prupe.1G431400_v2.0.a1
pyrus_communis pycom15g07260
rosa_chinensis RchiOBHm_Chr6g0308461
rosa_laevigata RLG00000010641
rosa_multiflora Rmu_co8427491.1_g000001 Rmu_sc0011796.1_g000007
rosa_roxburghii Rroxscaffold_7G00160110
rosa_rugosa Rorug06G0367000
rosa_samantha Rh6AG476700 Rh6BG486900 Rh6CG492000 Rh6DG478100
rosa_wichuraiana Rw6G041580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 66
Acc36I ACCTGC 2 cut(s) 305, 385
AccB1I GGYRCC 1 cut(s) 37
AciI CCGC 5 cut(s) 35, 134, 263, 377, 469
AcoI YGGCCR 1 cut(s) 52
AcyI GRCGYC 2 cut(s) 38, 63
AfaI GTAC 1 cut(s) 209
AfeI AGCGCT 1 cut(s) 232
AfiI CCNNNNNNNGG 2 cut(s) 133, 338
AgsI TTSAA 1 cut(s) 131
AjnI CCWGG 2 cut(s) 120, 343
AluBI AGCT 5 cut(s) 97, 205, 251, 284, 429
AluI AGCT 5 cut(s) 97, 205, 251, 284, 429
Alw21I GWGCWC 2 cut(s) 199, 483
Alw26I GTCTC 2 cut(s) 23, 93
Aor51HI AGCGCT 1 cut(s) 232
AoxI GGCC 2 cut(s) 52, 123
ApeKI GCWGC 3 cut(s) 233, 236, 311
AspLEI GCGC 2 cut(s) 40, 233
AspS9I GGNCC 4 cut(s) 124, 329, 341, 347
AsuHPI GGTGA 1 cut(s) 458
AvaII GGWCC 3 cut(s) 329, 341, 347
BanI GGYRCC 1 cut(s) 37
BauI CACGAG 1 cut(s) 198
BbsI GAAGAC 1 cut(s) 302
Bbv12I GWGCWC 2 cut(s) 199, 483
BbvI GCAGC 3 cut(s) 220, 223, 323
BccI CCATC 2 cut(s) 19, 413
BceAI ACGGC 2 cut(s) 25, 48
BciT130I CCWGG 2 cut(s) 122, 345
BclI TGATCA 2 cut(s) 241, 355
BcoDI GTCTC 2 cut(s) 23, 93
BfaI CTAG 2 cut(s) 248, 455
BfoI RGCGCY 2 cut(s) 41, 234
BfuAI ACCTGC 2 cut(s) 305, 385
BisI GCNGC 4 cut(s) 36, 234, 237, 312
BlsI GCNGC 4 cut(s) 37, 235, 238, 313
Bme1390I CCNGG 2 cut(s) 122, 345
Bme18I GGWCC 3 cut(s) 329, 341, 347
BmgT120I GGNCC 4 cut(s) 124, 329, 341, 347
BmiI GGNNCC 1 cut(s) 39
BmrFI CCNGG 2 cut(s) 122, 345
BmsI GCATC 2 cut(s) 381, 397
BpiI GAAGAC 1 cut(s) 302
BsaHI GRCGYC 2 cut(s) 38, 63
BsaJI CCNNGG 3 cut(s) 55, 120, 290
BsaXI ACNNNNNCTCC 2 cut(s) 441, 471
Bsc4I CCNNNNNNNGG 2 cut(s) 133, 338
Bse118I RCCGGY 1 cut(s) 50
Bse1I ACTGG 3 cut(s) 378, 388, 497
Bse3DI GCAATG 1 cut(s) 153
BseBI CCWGG 2 cut(s) 122, 345
BseDI CCNNGG 3 cut(s) 55, 120, 290
BseGI GGATG 3 cut(s) 286, 396, 412
BseLI CCNNNNNNNGG 2 cut(s) 133, 338
BseMI GCAATG 1 cut(s) 153
BseNI ACTGG 3 cut(s) 378, 388, 497
BseRI GAGGAG 1 cut(s) 182
BseXI GCAGC 3 cut(s) 220, 223, 323
BshFI GGCC 2 cut(s) 54, 125
BshNI GGYRCC 1 cut(s) 37
BsiHKAI GWGCWC 2 cut(s) 199, 483
BsiSI CCGG 1 cut(s) 51
BslFI GGGAC 1 cut(s) 86
BslI CCNNNNNNNGG 2 cut(s) 133, 338
BsmAI GTCTC 2 cut(s) 23, 93
BsmBI CGTCTC 1 cut(s) 23
BsmFI GGGAC 1 cut(s) 86
BsnI GGCC 2 cut(s) 54, 125
Bsp1286I GDGCHC 2 cut(s) 199, 483
Bsp1407I TGTACA 1 cut(s) 207
Bsp143I GATC 2 cut(s) 241, 355
BspACI CCGC 5 cut(s) 35, 134, 263, 377, 469
BspANI GGCC 2 cut(s) 54, 125
BspLI GGNNCC 1 cut(s) 39
BspMI ACCTGC 2 cut(s) 305, 385
BspT107I GGYRCC 1 cut(s) 37
BsrDI GCAATG 1 cut(s) 153
BsrFI RCCGGY 1 cut(s) 50
BsrGI TGTACA 1 cut(s) 207
BsrI ACTGG 3 cut(s) 378, 388, 497
BssAI RCCGGY 1 cut(s) 50
BssECI CCNNGG 3 cut(s) 55, 120, 290
BssMI GATC 2 cut(s) 241, 355
BssNI GRCGYC 2 cut(s) 38, 63
BssSI CACGAG 1 cut(s) 198
BssT1I CCWWGG 2 cut(s) 55, 290
Bst2BI CACGAG 1 cut(s) 198
Bst2UI CCWGG 2 cut(s) 122, 345
Bst4CI ACNGT 1 cut(s) 444
BstACI GRCGYC 2 cut(s) 38, 63
BstAUI TGTACA 1 cut(s) 207
BstC8I GCNNGC 2 cut(s) 95, 113
BstF5I GGATG 3 cut(s) 286, 396, 412
BstH2I RGCGCY 2 cut(s) 41, 234
BstHHI GCGC 2 cut(s) 40, 233
BstKTI GATC 2 cut(s) 244, 358
BstMAI GTCTC 2 cut(s) 23, 93
BstMBI GATC 2 cut(s) 241, 355
BstMWI GCNNNNNNNGC 3 cut(s) 131, 269, 468
BstNI CCWGG 2 cut(s) 122, 345
BstSCI CCNGG 2 cut(s) 120, 343
BstV1I GCAGC 3 cut(s) 220, 223, 323
BstV2I GAAGAC 1 cut(s) 302
BsuRI GGCC 2 cut(s) 54, 125
BtsCI GGATG 3 cut(s) 286, 396, 412
BtsIMutI CAGTG 1 cut(s) 449
BveI ACCTGC 2 cut(s) 305, 385
Cac8I GCNNGC 2 cut(s) 95, 113
CfoI GCGC 2 cut(s) 40, 233
Cfr10I RCCGGY 1 cut(s) 50
Cfr13I GGNCC 4 cut(s) 124, 329, 341, 347
Csp6I GTAC 1 cut(s) 208
CviJI RGCY 9 cut(s) 54, 97, 115, 125, 173, 205, 251, 284, 429
CviKI_1 RGCY 9 cut(s) 54, 97, 115, 125, 173, 205, 251, 284, 429
CviQI GTAC 1 cut(s) 208
DinI GGCGCC 1 cut(s) 39
DpnI GATC 2 cut(s) 243, 357
DpnII GATC 2 cut(s) 241, 355
EaeI YGGCCR 1 cut(s) 52
EciI GGCGGA 1 cut(s) 392
Eco130I CCWWGG 2 cut(s) 55, 290
Eco47I GGWCC 3 cut(s) 329, 341, 347
Eco47III AGCGCT 1 cut(s) 232
EcoO109I RGGNCCY 1 cut(s) 124
EcoRII CCWGG 2 cut(s) 120, 343
EcoT14I CCWWGG 2 cut(s) 55, 290
EcoT22I ATGCAT 1 cut(s) 412
EgeI GGCGCC 1 cut(s) 39
EheI GGCGCC 1 cut(s) 39
ErhI CCWWGG 2 cut(s) 55, 290
Esp3I CGTCTC 1 cut(s) 23
FaiI YATR 4 cut(s) 9, 143, 371, 490
FaqI GGGAC 1 cut(s) 86
FbaI TGATCA 2 cut(s) 241, 355
Fnu4HI GCNGC 4 cut(s) 36, 234, 237, 312
FokI GGATG 3 cut(s) 273, 403, 419
Fsp4HI GCNGC 4 cut(s) 36, 234, 237, 312
FspBI CTAG 2 cut(s) 248, 455
GlaI GCGC 2 cut(s) 39, 232
GluI GCNGC 4 cut(s) 36, 234, 237, 312
HaeII RGCGCY 2 cut(s) 41, 234
HaeIII GGCC 2 cut(s) 54, 125
HapII CCGG 1 cut(s) 51
HhaI GCGC 2 cut(s) 40, 233
Hin1I GRCGYC 2 cut(s) 38, 63
Hin6I GCGC 2 cut(s) 38, 231
HinP1I GCGC 2 cut(s) 38, 231
HpaII CCGG 1 cut(s) 51
HphI GGTGA 1 cut(s) 458
Hpy166II GTNNAC 1 cut(s) 440
Hpy188I TCNGA 1 cut(s) 418
Hpy188III TCNNGA 1 cut(s) 200
Hpy8I GTNNAC 1 cut(s) 440
Hpy99I CGWCG 2 cut(s) 65, 68
HpyAV CCTTC 2 cut(s) 126, 137
HpyCH4III ACNGT 1 cut(s) 444
HpyCH4IV ACGT 2 cut(s) 63, 321
HpyCH4V TGCA 4 cut(s) 223, 272, 394, 410
HpyF10VI GCNNNNNNNGC 3 cut(s) 131, 269, 468
HpySE526I ACGT 2 cut(s) 63, 321
Hsp92I GRCGYC 2 cut(s) 38, 63
HspAI GCGC 2 cut(s) 38, 231
KasI GGCGCC 1 cut(s) 37
Ksp22I TGATCA 2 cut(s) 241, 355
Kzo9I GATC 2 cut(s) 241, 355
LmnI GCTCC 1 cut(s) 102
Lsp1109I GCAGC 3 cut(s) 220, 223, 323
LweI GCATC 2 cut(s) 381, 397
MaeI CTAG 2 cut(s) 248, 455
MaeII ACGT 2 cut(s) 63, 321
MaeIII GTNAC 1 cut(s) 317
MalI GATC 2 cut(s) 243, 357
MboI GATC 2 cut(s) 241, 355
MboII GAAGA 1 cut(s) 307
MfeI CAATTG 1 cut(s) 273
MhlI GDGCHC 2 cut(s) 199, 483
MluCI AATT 1 cut(s) 273
Mly113I GGCGCC 1 cut(s) 38
MmeI TCCRAC 2 cut(s) 69, 384
MnlI CCTC 3 cut(s) 160, 163, 443
Mph1103I ATGCAT 1 cut(s) 412
MseI TTAA 1 cut(s) 102
MslI CAYNNNNRTG 2 cut(s) 20, 493
MspI CCGG 1 cut(s) 51
MspR9I CCNGG 2 cut(s) 122, 345
MunI CAATTG 1 cut(s) 273
MvaI CCWGG 2 cut(s) 122, 345
MwoI GCNNNNNNNGC 3 cut(s) 131, 269, 468
NarI GGCGCC 1 cut(s) 38
NdeII GATC 2 cut(s) 241, 355
NlaIV GGNNCC 1 cut(s) 39
NsiI ATGCAT 1 cut(s) 412
PcsI WCGNNNNNNNCGW 1 cut(s) 38
PflFI GACNNNGTC 1 cut(s) 65
PfoI TCCNGGA 1 cut(s) 343
PkrI GCNGC 4 cut(s) 37, 235, 238, 313
PluTI GGCGCC 1 cut(s) 41
Psp6I CCWGG 2 cut(s) 120, 343
PspGI CCWGG 2 cut(s) 120, 343
PspN4I GGNNCC 1 cut(s) 39
PspPI GGNCC 4 cut(s) 124, 329, 341, 347
PsyI GACNNNGTC 1 cut(s) 65
RsaI GTAC 1 cut(s) 209
RsaNI GTAC 1 cut(s) 208
RseI CAYNNNNRTG 2 cut(s) 20, 493
SaqAI TTAA 1 cut(s) 102
SatI GCNGC 4 cut(s) 36, 234, 237, 312
Sau3AI GATC 2 cut(s) 241, 355
Sau96I GGNCC 4 cut(s) 124, 329, 341, 347
ScrFI CCNGG 2 cut(s) 122, 345
SduI GDGCHC 2 cut(s) 199, 483
SfaNI GCATC 2 cut(s) 381, 397
SfoI GGCGCC 1 cut(s) 39
SinI GGWCC 3 cut(s) 329, 341, 347
SmiMI CAYNNNNRTG 2 cut(s) 20, 493
Sse9I AATT 1 cut(s) 273
SsiI CCGC 5 cut(s) 35, 134, 263, 377, 469
SspDI GGCGCC 1 cut(s) 37
SspMI CTAG 2 cut(s) 248, 455
StyD4I CCNGG 2 cut(s) 120, 343
StyI CCWWGG 2 cut(s) 55, 290
TaaI ACNGT 1 cut(s) 444
TaiI ACGT 2 cut(s) 66, 324
TasI AATT 1 cut(s) 273
TatI WGTACW 1 cut(s) 207
TauI GCSGC 1 cut(s) 38
Tru1I TTAA 1 cut(s) 102
Tru9I TTAA 1 cut(s) 102
TscAI CASTG 1 cut(s) 449
TseI GCWGC 3 cut(s) 233, 236, 311
TspDTI ATGAA 2 cut(s) 17, 421
TspGWI ACGGA 3 cut(s) 26, 32, 321
TspRI CASTG 1 cut(s) 449
Tth111I GACNNNGTC 1 cut(s) 65
VpaK11BI GGWCC 3 cut(s) 329, 341, 347
XspI CTAG 2 cut(s) 248, 455
ZraI GACGTC 1 cut(s) 64
Zsp2I ATGCAT 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.