MD08G1095400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0010893
Physical Location & Seq
Forward (+)
7987192 .. 7989220
2029 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1095400.v1.1.491

Sequence Viewer

Length: 534 bp
ATGAACCATACGGATATGGAAATGATGGAGGTGGAGACGACGGCGGCTGGGCGGCCGGAGCTGCCGTCCAGCACCGGCCAAGGTGACGTCGCCAGGGACTTGCTCACATTGTCTCGCCAGCTCCTCAATCAAGGCAAGCCCTCCCAGGCCCTCCAAGCGGTTGTTATGGCAATGAGAACAAGAGGTGGGGACGAGGCTGTATTTCAGTCCTTGCATCGTGCTCGTGAGCTGTACAAAAGTAGATTGCAAGAAAGCTCTGCTGCTGATCAACTAGCTTCTTTGTTTGCCGAGTGTGCAATTGCCGAAGCTCATCCTTCGAAGACTGAACCAGAAGCAGCATGTAACATGGGTGGTCCATCGGTTGGTCTTGGACCCGATGGTCAAGGAAGTTCCATACTGGCGGAAACTGGCAGGATGCAGATTGTTTTGGATGCATTTTCAGATGGGAGTAGCTTCATTTGTTTACAATGCGGAGGTCTTGTTAGCAATCACCGCAAAGACGAGCACTACGCATACTGGTGCTGCCAAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

178

Amino Acids

18.99

Weight (kDa)

5.04

Isoelectric Point (pI)

44.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C2HC_3 PF25017 130 - 175 2.9e-26 C2HC zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010893)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G47830 AT5G47830
fragaria_vesca FvH4_2g33950
malus_domestica MD06G1210300.v1.1 MD08G1095400.v1.1 MD15G1077900.v1.1 MD17G1062600.v1.1
prunus_persica Prupe.1G431400_v2.0.a1
pyrus_communis pycom15g07260
rosa_chinensis RchiOBHm_Chr6g0308461
rosa_laevigata RLG00000010641
rosa_multiflora Rmu_co8427491.1_g000001 Rmu_sc0011796.1_g000007
rosa_roxburghii Rroxscaffold_7G00160110
rosa_rugosa Rorug06G0367000
rosa_samantha Rh6AG476700 Rh6BG486900 Rh6CG492000 Rh6DG478100
rosa_wichuraiana Rw6G041580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 378
AatII GACGTC 1 cut(s) 90
AccB7I CCANNNNNTGG 1 cut(s) 362
AciI CCGC 6 cut(s) 44, 52, 158, 401, 471, 493
AcoI YGGCCR 2 cut(s) 53, 76
AcyI GRCGYC 1 cut(s) 87
AfaI GTAC 1 cut(s) 233
AfiI CCNNNNNNNGG 2 cut(s) 157, 362
AjnI CCWGG 2 cut(s) 92, 144
AluBI AGCT 7 cut(s) 61, 121, 229, 255, 275, 308, 453
AluI AGCT 7 cut(s) 61, 121, 229, 255, 275, 308, 453
Alw21I GWGCWC 2 cut(s) 223, 507
Alw26I GTCTC 2 cut(s) 29, 117
AoxI GGCC 3 cut(s) 53, 76, 147
ApeKI GCWGC 4 cut(s) 61, 260, 335, 522
AspS9I GGNCC 3 cut(s) 148, 353, 371
AsuHPI GGTGA 2 cut(s) 95, 482
AsuII TTCGAA 1 cut(s) 317
AvaII GGWCC 2 cut(s) 353, 371
BauI CACGAG 1 cut(s) 222
BbsI GAAGAC 1 cut(s) 326
Bbv12I GWGCWC 2 cut(s) 223, 507
BbvI GCAGC 4 cut(s) 48, 247, 347, 509
BccI CCATC 4 cut(s) 19, 364, 371, 437
BceAI ACGGC 2 cut(s) 49, 57
BcgI CGANNNNNNTGC 2 cut(s) 203, 237
BciT130I CCWGG 2 cut(s) 94, 146
BclI TGATCA 1 cut(s) 265
BcoDI GTCTC 2 cut(s) 29, 117
BfaI CTAG 1 cut(s) 272
BisI GCNGC 6 cut(s) 45, 53, 62, 261, 336, 523
BlsI GCNGC 6 cut(s) 46, 54, 63, 262, 337, 524
Bme1390I CCNGG 2 cut(s) 94, 146
Bme18I GGWCC 2 cut(s) 353, 371
BmgT120I GGNCC 3 cut(s) 148, 353, 371
BmiI GGNNCC 1 cut(s) 373
BmrFI CCNGG 2 cut(s) 94, 146
BmsI GCATC 3 cut(s) 223, 405, 421
BpiI GAAGAC 1 cut(s) 326
Bpu14I TTCGAA 1 cut(s) 317
BsaHI GRCGYC 1 cut(s) 87
BsaJI CCNNGG 3 cut(s) 79, 93, 144
BsaXI ACNNNNNCTCC 4 cut(s) 50, 80, 465, 495
Bsc4I CCNNNNNNNGG 2 cut(s) 157, 362
Bse118I RCCGGY 1 cut(s) 74
Bse1I ACTGG 3 cut(s) 402, 412, 521
Bse3DI GCAATG 1 cut(s) 177
BseBI CCWGG 2 cut(s) 94, 146
BseDI CCNNGG 3 cut(s) 79, 93, 144
BseGI GGATG 3 cut(s) 310, 420, 436
BseLI CCNNNNNNNGG 2 cut(s) 157, 362
BseMI GCAATG 1 cut(s) 177
BseNI ACTGG 3 cut(s) 402, 412, 521
BseRI GAGGAG 1 cut(s) 113
BseX3I CGGCCG 1 cut(s) 53
BseXI GCAGC 4 cut(s) 48, 247, 347, 509
BseYI CCCAGC 1 cut(s) 47
Bsh1285I CGRYCG 1 cut(s) 56
BshFI GGCC 3 cut(s) 55, 78, 149
BsiEI CGRYCG 1 cut(s) 56
BsiHKAI GWGCWC 2 cut(s) 223, 507
BsiSI CCGG 2 cut(s) 56, 75
BslFI GGGAC 2 cut(s) 110, 203
BslI CCNNNNNNNGG 2 cut(s) 157, 362
BsmAI GTCTC 2 cut(s) 29, 117
BsmBI CGTCTC 1 cut(s) 29
BsmFI GGGAC 2 cut(s) 110, 203
BsnI GGCC 3 cut(s) 55, 78, 149
Bsp119I TTCGAA 1 cut(s) 317
Bsp1286I GDGCHC 2 cut(s) 223, 507
Bsp1407I TGTACA 1 cut(s) 231
Bsp143I GATC 1 cut(s) 265
BspACI CCGC 6 cut(s) 44, 52, 158, 401, 471, 493
BspANI GGCC 3 cut(s) 55, 78, 149
BspLI GGNNCC 1 cut(s) 373
BspT104I TTCGAA 1 cut(s) 317
BsrDI GCAATG 1 cut(s) 177
BsrFI RCCGGY 1 cut(s) 74
BsrGI TGTACA 1 cut(s) 231
BsrI ACTGG 3 cut(s) 402, 412, 521
BssAI RCCGGY 1 cut(s) 74
BssECI CCNNGG 3 cut(s) 79, 93, 144
BssMI GATC 1 cut(s) 265
BssNI GRCGYC 1 cut(s) 87
BssSI CACGAG 1 cut(s) 222
BssT1I CCWWGG 1 cut(s) 79
Bst2BI CACGAG 1 cut(s) 222
Bst2UI CCWGG 2 cut(s) 94, 146
BstACI GRCGYC 1 cut(s) 87
BstAUI TGTACA 1 cut(s) 231
BstBI TTCGAA 1 cut(s) 317
BstC8I GCNNGC 2 cut(s) 119, 137
BstF5I GGATG 3 cut(s) 310, 420, 436
BstKTI GATC 1 cut(s) 268
BstMAI GTCTC 2 cut(s) 29, 117
BstMBI GATC 1 cut(s) 265
BstMCI CGRYCG 1 cut(s) 56
BstMWI GCNNNNNNNGC 5 cut(s) 58, 61, 155, 293, 492
BstNI CCWGG 2 cut(s) 94, 146
BstNSI RCATGY 1 cut(s) 342
BstSCI CCNGG 2 cut(s) 92, 144
BstV1I GCAGC 4 cut(s) 48, 247, 347, 509
BstV2I GAAGAC 1 cut(s) 326
BstZI CGGCCG 1 cut(s) 53
BsuRI GGCC 3 cut(s) 55, 78, 149
BtsCI GGATG 3 cut(s) 310, 420, 436
Cac8I GCNNGC 2 cut(s) 119, 137
Cfr10I RCCGGY 1 cut(s) 74
Cfr13I GGNCC 3 cut(s) 148, 353, 371
Csp6I GTAC 1 cut(s) 232
CviAII CATG 2 cut(s) 339, 346
CviQI GTAC 1 cut(s) 232
DpnI GATC 1 cut(s) 267
DpnII GATC 1 cut(s) 265
DrdI GACNNNNNNGTC 1 cut(s) 378
DseDI GACNNNNNNGTC 1 cut(s) 378
EaeI YGGCCR 2 cut(s) 53, 76
EagI CGGCCG 1 cut(s) 53
EciI GGCGGA 1 cut(s) 416
EclXI CGGCCG 1 cut(s) 53
Eco130I CCWWGG 1 cut(s) 79
Eco47I GGWCC 2 cut(s) 353, 371
Eco52I CGGCCG 1 cut(s) 53
EcoO109I RGGNCCY 1 cut(s) 148
EcoRII CCWGG 2 cut(s) 92, 144
EcoT14I CCWWGG 1 cut(s) 79
EcoT22I ATGCAT 1 cut(s) 436
ErhI CCWWGG 1 cut(s) 79
Esp3I CGTCTC 1 cut(s) 29
FaeI CATG 2 cut(s) 342, 349
FaiI YATR 7 cut(s) 9, 17, 167, 340, 347, 395, 514
FaqI GGGAC 2 cut(s) 110, 203
FatI CATG 2 cut(s) 338, 345
FbaI TGATCA 1 cut(s) 265
Fnu4HI GCNGC 6 cut(s) 45, 53, 62, 261, 336, 523
FokI GGATG 3 cut(s) 297, 427, 443
Fsp4HI GCNGC 6 cut(s) 45, 53, 62, 261, 336, 523
FspBI CTAG 1 cut(s) 272
GluI GCNGC 6 cut(s) 45, 53, 62, 261, 336, 523
GsaI CCCAGC 1 cut(s) 51
HaeIII GGCC 3 cut(s) 55, 78, 149
HapII CCGG 2 cut(s) 56, 75
Hin1I GRCGYC 1 cut(s) 87
Hin1II CATG 2 cut(s) 342, 349
HpaII CCGG 2 cut(s) 56, 75
HphI GGTGA 2 cut(s) 95, 482
Hpy166II GTNNAC 1 cut(s) 464
Hpy188I TCNGA 1 cut(s) 442
Hpy188III TCNNGA 1 cut(s) 224
Hpy8I GTNNAC 1 cut(s) 464
Hpy99I CGWCG 2 cut(s) 43, 92
HpyAV CCTTC 1 cut(s) 324
HpyCH4IV ACGT 1 cut(s) 87
HpyCH4V TGCA 5 cut(s) 214, 247, 296, 418, 434
HpyF10VI GCNNNNNNNGC 5 cut(s) 58, 61, 155, 293, 492
HpySE526I ACGT 1 cut(s) 87
Hsp92I GRCGYC 1 cut(s) 87
Hsp92II CATG 2 cut(s) 342, 349
Ksp22I TGATCA 1 cut(s) 265
Kzo9I GATC 1 cut(s) 265
LmnI GCTCC 2 cut(s) 58, 126
Lsp1109I GCAGC 4 cut(s) 48, 247, 347, 509
LweI GCATC 3 cut(s) 223, 405, 421
MaeI CTAG 1 cut(s) 272
MaeII ACGT 1 cut(s) 87
MaeIII GTNAC 2 cut(s) 83, 341
MalI GATC 1 cut(s) 267
MboI GATC 1 cut(s) 265
MboII GAAGA 1 cut(s) 331
MfeI CAATTG 1 cut(s) 297
MhlI GDGCHC 2 cut(s) 223, 507
MluCI AATT 1 cut(s) 297
MnlI CCTC 7 cut(s) 22, 134, 151, 161, 176, 187, 467
Mph1103I ATGCAT 1 cut(s) 436
MslI CAYNNNNRTG 1 cut(s) 517
MspI CCGG 2 cut(s) 56, 75
MspR9I CCNGG 2 cut(s) 94, 146
MunI CAATTG 1 cut(s) 297
MvaI CCWGG 2 cut(s) 94, 146
MwoI GCNNNNNNNGC 5 cut(s) 58, 61, 155, 293, 492
NdeII GATC 1 cut(s) 265
NlaIII CATG 2 cut(s) 342, 349
NlaIV GGNNCC 1 cut(s) 373
NmeAIII GCCGAG 1 cut(s) 313
NmuCI GTSAC 1 cut(s) 83
NsiI ATGCAT 1 cut(s) 436
NspI RCATGY 1 cut(s) 342
NspV TTCGAA 1 cut(s) 317
PflMI CCANNNNNTGG 1 cut(s) 362
PkrI GCNGC 6 cut(s) 46, 54, 63, 262, 337, 524
Psp6I CCWGG 2 cut(s) 92, 144
PspFI CCCAGC 1 cut(s) 47
PspGI CCWGG 2 cut(s) 92, 144
PspN4I GGNNCC 1 cut(s) 373
PspPI GGNCC 3 cut(s) 148, 353, 371
RsaI GTAC 1 cut(s) 233
RsaNI GTAC 1 cut(s) 232
RseI CAYNNNNRTG 1 cut(s) 517
SatI GCNGC 6 cut(s) 45, 53, 62, 261, 336, 523
Sau3AI GATC 1 cut(s) 265
Sau96I GGNCC 3 cut(s) 148, 353, 371
ScrFI CCNGG 2 cut(s) 94, 146
SduI GDGCHC 2 cut(s) 223, 507
SfaNI GCATC 3 cut(s) 223, 405, 421
SfuI TTCGAA 1 cut(s) 317
SinI GGWCC 2 cut(s) 353, 371
SmiMI CAYNNNNRTG 1 cut(s) 517
Sse9I AATT 1 cut(s) 297
SsiI CCGC 6 cut(s) 44, 52, 158, 401, 471, 493
SspMI CTAG 1 cut(s) 272
StyD4I CCNGG 2 cut(s) 92, 144
StyI CCWWGG 1 cut(s) 79
TaiI ACGT 1 cut(s) 90
TaqI TCGA 1 cut(s) 317
TasI AATT 1 cut(s) 297
TatI WGTACW 1 cut(s) 231
TauI GCSGC 2 cut(s) 47, 55
TseFI GTSAC 1 cut(s) 83
TseI GCWGC 4 cut(s) 61, 260, 335, 522
Tsp45I GTSAC 1 cut(s) 83
TspDTI ATGAA 2 cut(s) 17, 445
TspGWI ACGGA 1 cut(s) 26
Van91I CCANNNNNTGG 1 cut(s) 362
VpaK11BI GGWCC 2 cut(s) 353, 371
XceI RCATGY 1 cut(s) 342
XspI CTAG 1 cut(s) 272
ZraI GACGTC 1 cut(s) 88
Zsp2I ATGCAT 1 cut(s) 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.