FvH4_2g36010

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010531
Physical Location & Seq
Forward (+)
26457048 .. 26458802
1755 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g36010.t1

Sequence Viewer

Length: 723 bp
ATGGTAAACTGTGCAAGATGTTGCTTGCACAACTCAATGAGAGCTTTGAACTTCTTTGTCAATATCTTTGCTGTGGGGGTGATCATCTACACTCTCTGGCTGCTCAAGAAGTGGCAGGATGGAGTTACTGAGCTCCCTCCAGTCCCAATGGTACTTAAACCATGGTTTATCTATGCATGTTTAGGTGCGGGGATTGCCGTCTGTTTCAGCACACTTTTCAGTTATCTGGTTGCTCATTGCATCACTGATTCTACTCTTCTTCTCATATACATAGTTTCGATCTTCTCTCTTCTTTGCATTGAAGTTGCAGGGGTTGTAGCAATTTTCTACAAGATGAACTGGGCAGAGAAACTTGCAGAGTACATAAAGGACACCAAGTTCAAGAACTTCATGATATTTCATCTGTACATGTGTCGTCTCATTGCGATTCTAATTTTGGTACCGCAGATCAAATGCACAGTTTTAGCAATCATTCTTTGGGCTATTGGTACCGAGCCACTGACTCACTGCAACTATTCTGACCATGTAACTGAGTTTAGATATTATTCATTTCTGGTGATACCAAGCCCAGCTTCTCTACTCAATATGTCGAGAAATGGCTTCAGAAATTATGAAGCCTTGCCAAGATCAGATCAATATGAATATCCGCCACGGCTAAGCTTCTTCTCACGTATTAACAGATTTTTCAGAATGCGATTTCATAGAAGAGTCACACTTGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tetraspannin

Protein Analysis

241

Amino Acids

27.91

Weight (kDa)

9.19

Isoelectric Point (pI)

40.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 439, 488
AccB1I GGYRCC 2 cut(s) 439, 488
AciI CCGC 3 cut(s) 188, 443, 647
AcuI CTGAAG 1 cut(s) 586
AfaI GTAC 5 cut(s) 153, 362, 407, 441, 490
AflIII ACRYGT 1 cut(s) 408
AgsI TTSAA 3 cut(s) 49, 302, 382
AjuI GAANNNNNNNTTGG 2 cut(s) 556, 588
AloI GAACNNNNNNTCC 2 cut(s) 362, 394
AluBI AGCT 4 cut(s) 44, 133, 572, 660
AluI AGCT 4 cut(s) 44, 133, 572, 660
Alw21I GWGCWC 1 cut(s) 135
Alw26I GTCTC 1 cut(s) 422
ApeKI GCWGC 1 cut(s) 100
Asp718I GGTACC 2 cut(s) 439, 488
AsuHPI GGTGA 2 cut(s) 91, 568
BanI GGYRCC 2 cut(s) 439, 488
BanII GRGCYC 1 cut(s) 135
Bbv12I GWGCWC 1 cut(s) 135
BbvI GCAGC 1 cut(s) 87
BccI CCATC 1 cut(s) 113
BceAI ACGGC 2 cut(s) 182, 668
BclI TGATCA 1 cut(s) 81
BcoDI GTCTC 1 cut(s) 422
BisI GCNGC 1 cut(s) 101
BlpI GCTNAGC 1 cut(s) 656
BlsI GCNGC 1 cut(s) 102
BmiI GGNNCC 2 cut(s) 441, 490
BmrI ACTGGG 1 cut(s) 349
BmsI GCATC 1 cut(s) 249
BmuI ACTGGG 1 cut(s) 349
BpmI CTGGAG 1 cut(s) 123
Bpu1102I GCTNAGC 1 cut(s) 656
BpuEI CTTGAG 1 cut(s) 89
BsaAI YACGTR 1 cut(s) 671
BsaJI CCNNGG 2 cut(s) 161, 650
Bse1I ACTGG 2 cut(s) 140, 344
Bse3DI GCAATG 2 cut(s) 235, 420
BseDI CCNNGG 2 cut(s) 161, 650
BseGI GGATG 1 cut(s) 124
BseMI GCAATG 2 cut(s) 235, 420
BseMII CTCAG 2 cut(s) 120, 522
BseNI ACTGG 2 cut(s) 140, 344
BseXI GCAGC 1 cut(s) 87
BseYI CCCAGC 1 cut(s) 568
BshNI GGYRCC 2 cut(s) 439, 488
BsiHKAI GWGCWC 1 cut(s) 135
BslFI GGGAC 1 cut(s) 128
BsmAI GTCTC 1 cut(s) 422
BsmBI CGTCTC 1 cut(s) 422
BsmFI GGGAC 1 cut(s) 128
BsmI GAATGC 1 cut(s) 696
Bsp1286I GDGCHC 1 cut(s) 135
Bsp1407I TGTACA 1 cut(s) 405
Bsp143I GATC 5 cut(s) 81, 279, 447, 626, 631
Bsp1720I GCTNAGC 1 cut(s) 656
Bsp19I CCATGG 1 cut(s) 161
BspACI CCGC 3 cut(s) 188, 443, 647
BspCNI CTCAG 2 cut(s) 121, 523
BspHI TCATGA 1 cut(s) 390
BspLI GGNNCC 2 cut(s) 441, 490
BspT107I GGYRCC 2 cut(s) 439, 488
BsrDI GCAATG 2 cut(s) 235, 420
BsrGI TGTACA 1 cut(s) 405
BsrI ACTGG 2 cut(s) 140, 344
BssECI CCNNGG 2 cut(s) 161, 650
BssMI GATC 5 cut(s) 81, 279, 447, 626, 631
BssT1I CCWWGG 1 cut(s) 161
Bst4CI ACNGT 2 cut(s) 11, 460
Bst6I CTCTTC 3 cut(s) 261, 294, 700
BstAUI TGTACA 1 cut(s) 405
BstBAI YACGTR 1 cut(s) 671
BstC8I GCNNGC 1 cut(s) 26
BstDEI CTNAG 3 cut(s) 129, 531, 656
BstDSI CCRYGG 2 cut(s) 161, 650
BstF5I GGATG 1 cut(s) 124
BstKTI GATC 5 cut(s) 84, 282, 450, 629, 634
BstMAI GTCTC 1 cut(s) 422
BstMBI GATC 5 cut(s) 81, 279, 447, 626, 631
BstMWI GCNNNNNNNGC 1 cut(s) 194
BstNSI RCATGY 2 cut(s) 180, 412
BstV1I GCAGC 1 cut(s) 87
BtgI CCRYGG 2 cut(s) 161, 650
BtsCI GGATG 1 cut(s) 124
BtsI GCAGTG 1 cut(s) 505
BtsIMutI CAGTG 3 cut(s) 243, 497, 505
Cac8I GCNNGC 1 cut(s) 26
CciI TCATGA 1 cut(s) 390
Csp6I GTAC 5 cut(s) 152, 361, 406, 440, 489
CviAII CATG 5 cut(s) 162, 177, 391, 409, 524
CviQI GTAC 5 cut(s) 152, 361, 406, 440, 489
DdeI CTNAG 3 cut(s) 129, 531, 656
DpnI GATC 5 cut(s) 83, 281, 449, 628, 633
DpnII GATC 5 cut(s) 81, 279, 447, 626, 631
Eam1104I CTCTTC 3 cut(s) 261, 294, 700
EarI CTCTTC 3 cut(s) 261, 294, 700
EciI GGCGGA 1 cut(s) 636
Ecl136II GAGCTC 1 cut(s) 133
Eco130I CCWWGG 1 cut(s) 161
Eco24I GRGCYC 1 cut(s) 135
Eco53kI GAGCTC 1 cut(s) 133
Eco57I CTGAAG 1 cut(s) 586
EcoICRI GAGCTC 1 cut(s) 133
EcoT14I CCWWGG 1 cut(s) 161
EcoT22I ATGCAT 1 cut(s) 178
EcoT38I GRGCYC 1 cut(s) 135
ErhI CCWWGG 1 cut(s) 161
Esp3I CGTCTC 1 cut(s) 422
FaeI CATG 5 cut(s) 165, 180, 394, 412, 527
FalI AAGNNNNNCTT 2 cut(s) 556, 588
FaqI GGGAC 1 cut(s) 128
FatI CATG 5 cut(s) 161, 176, 390, 408, 523
FauI CCCGC 1 cut(s) 181
FbaI TGATCA 1 cut(s) 81
Fnu4HI GCNGC 1 cut(s) 101
FokI GGATG 1 cut(s) 131
FriOI GRGCYC 1 cut(s) 135
Fsp4HI GCNGC 1 cut(s) 101
GluI GCNGC 1 cut(s) 101
GsaI CCCAGC 1 cut(s) 572
GsuI CTGGAG 1 cut(s) 123
Hin1II CATG 5 cut(s) 165, 180, 394, 412, 527
HindIII AAGCTT 1 cut(s) 658
HinfI GANTC 4 cut(s) 248, 427, 502, 708
HphI GGTGA 2 cut(s) 91, 568
Hpy166II GTNNAC 1 cut(s) 7
Hpy188I TCNGA 4 cut(s) 520, 605, 631, 689
Hpy188III TCNNGA 4 cut(s) 106, 382, 391, 591
Hpy8I GTNNAC 1 cut(s) 7
HpyCH4III ACNGT 2 cut(s) 11, 460
HpyCH4IV ACGT 1 cut(s) 670
HpyCH4V TGCA 9 cut(s) 14, 28, 176, 240, 297, 308, 356, 456, 510
HpyF10VI GCNNNNNNNGC 1 cut(s) 194
HpyF3I CTNAG 3 cut(s) 129, 531, 656
HpySE526I ACGT 1 cut(s) 670
Hsp92II CATG 5 cut(s) 165, 180, 394, 412, 527
KpnI GGTACC 2 cut(s) 443, 492
Ksp22I TGATCA 1 cut(s) 81
Kzo9I GATC 5 cut(s) 81, 279, 447, 626, 631
LmnI GCTCC 1 cut(s) 138
LpnPI CCDG 8 cut(s) 82, 101, 153, 212, 294, 325, 539, 582
Lsp1109I GCAGC 1 cut(s) 87
LweI GCATC 1 cut(s) 249
MaeII ACGT 1 cut(s) 670
MaeIII GTNAC 3 cut(s) 124, 526, 709
MalI GATC 5 cut(s) 83, 281, 449, 628, 633
MboI GATC 5 cut(s) 81, 279, 447, 626, 631
MboII GAAGA 6 cut(s) 248, 251, 274, 281, 655, 717
MhlI GDGCHC 1 cut(s) 135
MluCI AATT 3 cut(s) 321, 432, 607
MlyI GAGTC 2 cut(s) 496, 717
MnlI CCTC 1 cut(s) 147
Mph1103I ATGCAT 1 cut(s) 178
MseI TTAA 2 cut(s) 156, 675
Mva1269I GAATGC 1 cut(s) 696
MwoI GCNNNNNNNGC 1 cut(s) 194
NcoI CCATGG 1 cut(s) 161
NdeII GATC 5 cut(s) 81, 279, 447, 626, 631
NlaIII CATG 5 cut(s) 165, 180, 394, 412, 527
NlaIV GGNNCC 2 cut(s) 441, 490
NmuCI GTSAC 1 cut(s) 709
NsiI ATGCAT 1 cut(s) 178
NspI RCATGY 2 cut(s) 180, 412
PagI TCATGA 1 cut(s) 390
PciI ACATGT 1 cut(s) 408
PctI GAATGC 1 cut(s) 696
PfeI GAWTC 2 cut(s) 248, 427
PkrI GCNGC 1 cut(s) 102
PleI GAGTC 2 cut(s) 496, 716
PpsI GAGTC 2 cut(s) 496, 716
Ppu21I YACGTR 1 cut(s) 671
PscI ACATGT 1 cut(s) 408
Psp124BI GAGCTC 1 cut(s) 135
PspFI CCCAGC 1 cut(s) 568
PspN4I GGNNCC 2 cut(s) 441, 490
RsaI GTAC 5 cut(s) 153, 362, 407, 441, 490
RsaNI GTAC 5 cut(s) 152, 361, 406, 440, 489
SacI GAGCTC 1 cut(s) 135
SaqAI TTAA 2 cut(s) 156, 675
SatI GCNGC 1 cut(s) 101
Sau3AI GATC 5 cut(s) 81, 279, 447, 626, 631
SchI GAGTC 2 cut(s) 496, 717
SduI GDGCHC 1 cut(s) 135
SetI ASST 6 cut(s) 46, 135, 187, 574, 662, 673
SfaNI GCATC 1 cut(s) 249
SmlI CTYRAG 1 cut(s) 104
SmoI CTYRAG 1 cut(s) 104
Sse9I AATT 3 cut(s) 321, 432, 607
SsiI CCGC 3 cut(s) 188, 443, 647
SstI GAGCTC 1 cut(s) 135
StyI CCWWGG 1 cut(s) 161
TaaI ACNGT 2 cut(s) 11, 460
TaiI ACGT 1 cut(s) 673
TaqI TCGA 2 cut(s) 278, 590
TasI AATT 3 cut(s) 321, 432, 607
TatI WGTACW 2 cut(s) 360, 405
TfiI GAWTC 2 cut(s) 248, 427
Tru1I TTAA 2 cut(s) 156, 675
Tru9I TTAA 2 cut(s) 156, 675
TscAI CASTG 3 cut(s) 250, 504, 512
TseFI GTSAC 1 cut(s) 709
TseI GCWGC 1 cut(s) 100
Tsp45I GTSAC 1 cut(s) 709
TspDTI ATGAA 7 cut(s) 350, 379, 389, 537, 627, 654, 689
TspRI CASTG 3 cut(s) 250, 504, 512
XceI RCATGY 2 cut(s) 180, 412
Zsp2I ATGCAT 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.