MD08G1117200.v1.1

Aspartate aminotransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0010531
Physical Location & Seq
Forward (+)
10729843 .. 10731898
2056 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1117200.v1.1.491

Sequence Viewer

Length: 459 bp
ATGGTGGGCAGTGCAAGGCTTTGCTTGCACAACTCCATGAGAGCTGTGAATTTATTTGTCAACGTTTGTGGAGTTGGAGTCATCATCTACTCCCTTTGGCTCATCAAGAAATGGCAAGTTGGAGTCTCTGAGCTCCCTCCCGTCGCGCATGTCCCCAAGCCATGGTTTATATATACATGTTTAGGTATGGGAATTGCTGTCTGCCTGAGCACCCTTTGCGGTCATATGGTTGCCAATTCCATCAGCAGTTTCATTCTCTTCATTTACATAATCTCCATCTTCTGTCTTCTTGGCATTGAGGTTGGAGTGATCGTCAATATTTTCTATAGGATGGACTGGGAAGTGCAATTTTCAAAGTACATTGATGAGCATCACACCCTTTTCAAAAACTTCTTGACATTTCATCTAAACATGTGCCGCCTCATTGTAGTTCTCATCTTGATACCACAGGTAAAATAA

Protein Analysis

153

Amino Acids

17.29

Weight (kDa)

8.73

Isoelectric Point (pI)

25.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 13 - 121 2e-06 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 162
AccII CGCG 1 cut(s) 146
AciI CCGC 2 cut(s) 219, 418
AclI AACGTT 1 cut(s) 63
AcsI RAATTY 1 cut(s) 49
AfaI GTAC 1 cut(s) 359
AfiI CCNNNNNNNGG 1 cut(s) 162
AflIII ACRYGT 2 cut(s) 176, 411
AgsI TTSAA 2 cut(s) 354, 385
AluBI AGCT 2 cut(s) 44, 133
AluI AGCT 2 cut(s) 44, 133
Alw21I GWGCWC 2 cut(s) 135, 212
Alw26I GTCTC 1 cut(s) 130
ApoI RAATTY 1 cut(s) 49
AspLEI GCGC 1 cut(s) 148
BanII GRGCYC 1 cut(s) 135
BbsI GAAGAC 1 cut(s) 278
Bbv12I GWGCWC 2 cut(s) 135, 212
BccI CCATC 3 cut(s) 248, 284, 325
BcoDI GTCTC 1 cut(s) 130
BfmI CTRYAG 1 cut(s) 325
BisI GCNGC 1 cut(s) 418
BlsI GCNGC 1 cut(s) 419
BmrI ACTGGG 1 cut(s) 346
BmsI GCATC 1 cut(s) 379
BmuI ACTGGG 1 cut(s) 346
BpiI GAAGAC 1 cut(s) 278
Bpu10I CCTNAGC 1 cut(s) 206
BsaBI GATNNNNATC 1 cut(s) 369
BsaJI CCNNGG 1 cut(s) 161
BsaXI ACNNNNNCTCC 6 cut(s) 63, 93, 257, 287, 297, 327
Bsc4I CCNNNNNNNGG 1 cut(s) 162
Bse1I ACTGG 1 cut(s) 341
Bse8I GATNNNNATC 1 cut(s) 369
BseDI CCNNGG 1 cut(s) 161
BseGI GGATG 1 cut(s) 336
BseJI GATNNNNATC 1 cut(s) 369
BseLI CCNNNNNNNGG 1 cut(s) 162
BseMII CTCAG 2 cut(s) 120, 197
BseNI ACTGG 1 cut(s) 341
Bsh1236I CGCG 1 cut(s) 146
BsiHKAI GWGCWC 2 cut(s) 135, 212
BslFI GGGAC 1 cut(s) 137
BslI CCNNNNNNNGG 1 cut(s) 162
BsmAI GTCTC 1 cut(s) 130
BsmFI GGGAC 1 cut(s) 137
Bsp1286I GDGCHC 2 cut(s) 135, 212
Bsp143I GATC 1 cut(s) 309
Bsp19I CCATGG 1 cut(s) 161
BspACI CCGC 2 cut(s) 219, 418
BspCNI CTCAG 2 cut(s) 121, 198
BspFNI CGCG 1 cut(s) 146
BsrI ACTGG 1 cut(s) 341
BssECI CCNNGG 1 cut(s) 161
BssMI GATC 1 cut(s) 309
BssT1I CCWWGG 1 cut(s) 161
Bst6I CTCTTC 1 cut(s) 263
BstAPI GCANNNNNTGC 1 cut(s) 216
BstC8I GCNNGC 1 cut(s) 26
BstDEI CTNAG 2 cut(s) 129, 206
BstDSI CCRYGG 1 cut(s) 161
BstF5I GGATG 1 cut(s) 336
BstFNI CGCG 1 cut(s) 146
BstHHI GCGC 1 cut(s) 148
BstKTI GATC 1 cut(s) 312
BstMAI GTCTC 1 cut(s) 130
BstMBI GATC 1 cut(s) 309
BstMWI GCNNNNNNNGC 2 cut(s) 25, 216
BstNSI RCATGY 3 cut(s) 152, 180, 415
BstSFI CTRYAG 1 cut(s) 325
BstUI CGCG 1 cut(s) 146
BstV2I GAAGAC 1 cut(s) 278
BtgI CCRYGG 1 cut(s) 161
BtsCI GGATG 1 cut(s) 336
BtsI GCAGTG 1 cut(s) 16
BtsIMutI CAGTG 1 cut(s) 16
Cac8I GCNNGC 1 cut(s) 26
CfoI GCGC 1 cut(s) 148
Csp6I GTAC 1 cut(s) 358
CspCI CAANNNNNGTGG 2 cut(s) 49, 84
CviAII CATG 5 cut(s) 37, 149, 162, 177, 412
CviJI RGCY 5 cut(s) 19, 44, 100, 133, 160
CviKI_1 RGCY 5 cut(s) 19, 44, 100, 133, 160
CviQI GTAC 1 cut(s) 358
DdeI CTNAG 2 cut(s) 129, 206
DpnI GATC 1 cut(s) 311
DpnII GATC 1 cut(s) 309
Eam1104I CTCTTC 1 cut(s) 263
EarI CTCTTC 1 cut(s) 263
Ecl136II GAGCTC 1 cut(s) 133
Eco130I CCWWGG 1 cut(s) 161
Eco24I GRGCYC 1 cut(s) 135
Eco53kI GAGCTC 1 cut(s) 133
EcoICRI GAGCTC 1 cut(s) 133
EcoT14I CCWWGG 1 cut(s) 161
EcoT38I GRGCYC 1 cut(s) 135
ErhI CCWWGG 1 cut(s) 161
FaeI CATG 5 cut(s) 40, 152, 165, 180, 415
FaqI GGGAC 1 cut(s) 137
FatI CATG 5 cut(s) 36, 148, 161, 176, 411
FauNDI CATATG 1 cut(s) 225
Fnu4HI GCNGC 1 cut(s) 418
FokI GGATG 1 cut(s) 343
FriOI GRGCYC 1 cut(s) 135
Fsp4HI GCNGC 1 cut(s) 418
GlaI GCGC 1 cut(s) 147
GluI GCNGC 1 cut(s) 418
HhaI GCGC 1 cut(s) 148
Hin1II CATG 5 cut(s) 40, 152, 165, 180, 415
Hin6I GCGC 1 cut(s) 146
HinP1I GCGC 1 cut(s) 146
HincII GTYRAC 1 cut(s) 61
HindII GTYRAC 1 cut(s) 61
HinfI GANTC 2 cut(s) 78, 123
Hpy166II GTNNAC 1 cut(s) 61
Hpy188I TCNGA 1 cut(s) 130
Hpy188III TCNNGA 3 cut(s) 106, 394, 439
Hpy8I GTNNAC 1 cut(s) 61
Hpy99I CGWCG 1 cut(s) 146
HpyCH4IV ACGT 1 cut(s) 63
HpyCH4V TGCA 3 cut(s) 14, 28, 346
HpyF10VI GCNNNNNNNGC 2 cut(s) 25, 216
HpyF3I CTNAG 2 cut(s) 129, 206
HpySE526I ACGT 1 cut(s) 63
Hsp92II CATG 5 cut(s) 40, 152, 165, 180, 415
HspAI GCGC 1 cut(s) 146
Kzo9I GATC 1 cut(s) 309
LmnI GCTCC 1 cut(s) 138
LpnPI CCDG 3 cut(s) 218, 322, 434
LweI GCATC 1 cut(s) 379
MaeII ACGT 1 cut(s) 63
MalI GATC 1 cut(s) 311
MboI GATC 1 cut(s) 309
MboII GAAGA 3 cut(s) 250, 271, 278
MhlI GDGCHC 2 cut(s) 135, 212
MluCI AATT 4 cut(s) 49, 192, 235, 347
MlyI GAGTC 2 cut(s) 87, 132
MmeI TCCRAC 3 cut(s) 55, 100, 283
MnlI CCTC 3 cut(s) 147, 292, 431
MvnI CGCG 1 cut(s) 146
MwoI GCNNNNNNNGC 2 cut(s) 25, 216
NcoI CCATGG 1 cut(s) 161
NdeI CATATG 1 cut(s) 225
NdeII GATC 1 cut(s) 309
NlaIII CATG 5 cut(s) 40, 152, 165, 180, 415
NspI RCATGY 3 cut(s) 152, 180, 415
PciI ACATGT 2 cut(s) 176, 411
PflMI CCANNNNNTGG 1 cut(s) 162
PkrI GCNGC 1 cut(s) 419
PleI GAGTC 2 cut(s) 86, 131
PpsI GAGTC 2 cut(s) 86, 131
PscI ACATGT 2 cut(s) 176, 411
Psp124BI GAGCTC 1 cut(s) 135
Psp1406I AACGTT 1 cut(s) 63
RsaI GTAC 1 cut(s) 359
RsaNI GTAC 1 cut(s) 358
SacI GAGCTC 1 cut(s) 135
SatI GCNGC 1 cut(s) 418
Sau3AI GATC 1 cut(s) 309
SchI GAGTC 2 cut(s) 87, 132
SduI GDGCHC 2 cut(s) 135, 212
SetI ASST 6 cut(s) 46, 66, 135, 187, 303, 453
SfaNI GCATC 1 cut(s) 379
SfcI CTRYAG 1 cut(s) 325
Sse9I AATT 4 cut(s) 49, 192, 235, 347
SsiI CCGC 2 cut(s) 219, 418
SspI AATATT 1 cut(s) 319
SstI GAGCTC 1 cut(s) 135
StyI CCWWGG 1 cut(s) 161
TaiI ACGT 1 cut(s) 66
TasI AATT 4 cut(s) 49, 192, 235, 347
TatI WGTACW 1 cut(s) 357
TauI GCSGC 1 cut(s) 420
TscAI CASTG 1 cut(s) 16
TspDTI ATGAA 3 cut(s) 241, 250, 392
TspRI CASTG 1 cut(s) 16
Van91I CCANNNNNTGG 1 cut(s) 162
XapI RAATTY 1 cut(s) 49
XceI RCATGY 3 cut(s) 152, 180, 415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.