FvH4_2g36751

domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0005356
Physical Location & Seq
Reverse (-)
26856853 .. 26859450
2598 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g36751.t2

Sequence Viewer

Length: 1302 bp
ATGGCAACTAAATCAAAAGGTAGTACAACTCCTGGGAGGGAGAAGAGAAGTGGCAGCACATCACCAGCTAGCCTCACCAGAAGACCCACAAAACCAACTGCTCCTGATAAACCATCTTCAACTTCCTCAAACTCATCATCAACAGCTAAACAACTCCCAAATTATTTGAGATCAACCGCAAGCTCATCGCGCCAGCTTCCTTCCTTGACCAAGTCCAGTTCATTAAAGAAACATCATGATTCTGATAACCAAGAATCCAAGTCCCCAAAGCCTACACTAAATAGAAGAAGATCCTTTGACAAGCCGCCGTCGCCTTCAGGACTACAGAAGTCACTCAACTCCCCAGGAAGAGAGTTGAAGGACGCACCACACCGGTCCAAATCTCTTTTGGTTAGAAGCGTGTCAAATGCTCCAAAACCCACCAGTAAATCTGTATCCGGAACCAAACCACAGGTTTCGCATGCAAAGACTAGTGGCACTAGTTTGAGGAGAAGCACTAGCGGCATTACTTCTGGTTCAAGTAAGAAGGATAGTAGAAATAATAGAAGTACTACTAGTACTACTGGTAGTTCAAGAGCTCCAAGGAGTCAGGACATAACAGAAATTCTCAATCTTGAAGCTAATTTGGATGCCTTGAATCGTCATGAGGTTGGAGAGGTAGATAAGATTGAGAGTATTGAGGAGGACAACTGTCAAGACCTTCCGGACCCTAAATTAGCTGAAGAAGATCTTCATGAACAGCACCAACATCATGCTTCAGATACTGGTCATGACATTTCTTCTGTTAATGATGCAGCAGAGGAATCAAAAGCAAATGTGGATGCCGAAAATGAAACAGTTATCCAAGCAGAGAAAAATTTTGATGGTCACCATGATCAAGTTGAAGAGGCTAATCACATTAGCAAAGATCATGAAAGCAATGGCATTGATCAGGGTTCGGGTTTGACAAGCGAGGAAACAAAAGTTGAGGAGGCGATCAAAGAAGAGAAAGAAGATGAAAACAAGAATGAGGAGGATATGGAAGTTGTGGATGGAGTAAGTCATGAGCTTGATCCAAAGGAAGGGCAAGATGGAGATGTCAAAGAAGAAGCAAAGCCTGACGAGGAAAAGGCGACAGAAAAGCCACAAGAGGCTGCGGGTACTGCAGGGAATAACAAGAAGGAGGCAGCTCCTGCATACAATGATGTGATTGAGGAGACTGCAAGTAAGCTTATGGCACAAGAGAAGAGGAGGAACAAGGTCAAAGCTTTGGTTGGGGCATTTGAGACTGTCATAGATTATGAATCAGGATCCAAACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaM_binding

Protein Analysis

434

Amino Acids

46.91

Weight (kDa)

5.69

Isoelectric Point (pI)

63.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaM_binding PF07839 320 - 425 4.2e-12 Plant calmodulin-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 190
AccIII TCCGGA 2 cut(s) 437, 703
AciI CCGC 4 cut(s) 177, 305, 501, 1136
AclWI GGATC 4 cut(s) 285, 1046, 1284, 1297
AcsI RAATTY 2 cut(s) 603, 856
AcuI CTGAAG 3 cut(s) 300, 741, 741
AfaI GTAC 4 cut(s) 25, 550, 559, 1141
AfiI CCNNNNNNNGG 1 cut(s) 1061
AgeI ACCGGT 1 cut(s) 372
AgsI TTSAA 7 cut(s) 120, 358, 519, 573, 617, 637, 884
AhlI ACTAGT 3 cut(s) 470, 479, 554
AjnI CCWGG 2 cut(s) 31, 343
Alw21I GWGCWC 1 cut(s) 580
Alw26I GTCTC 2 cut(s) 1190, 1259
AlwI GGATC 4 cut(s) 285, 1046, 1284, 1297
AlwNI CAGNNNCTG 2 cut(s) 764, 1172
Aor13HI TCCGGA 2 cut(s) 437, 703
ApeKI GCWGC 4 cut(s) 54, 794, 1133, 1166
ApoI RAATTY 2 cut(s) 603, 856
AsiGI ACCGGT 1 cut(s) 372
Asp700I GAANNNNTTC 1 cut(s) 729
AspLEI GCGC 1 cut(s) 192
AspS9I GGNCC 2 cut(s) 375, 706
AsuHPI GGTGA 3 cut(s) 54, 67, 860
AsuNHI GCTAGC 1 cut(s) 68
AvaII GGWCC 2 cut(s) 375, 706
BamHI GGATCC 1 cut(s) 1289
BanII GRGCYC 1 cut(s) 580
BbsI GAAGAC 1 cut(s) 88
Bbv12I GWGCWC 1 cut(s) 580
BbvI GCAGC 4 cut(s) 66, 806, 1120, 1178
BccI CCATC 4 cut(s) 121, 857, 1025, 1064
BceAI ACGGC 1 cut(s) 292
BcgI CGANNNNNNTGC 2 cut(s) 168, 202
BciT130I CCWGG 2 cut(s) 33, 345
BciVI GTATCC 1 cut(s) 445
BclI TGATCA 2 cut(s) 874, 928
BcoDI GTCTC 2 cut(s) 1190, 1259
BcuI ACTAGT 3 cut(s) 470, 479, 554
BfaI CTAG 5 cut(s) 69, 471, 480, 498, 555
BfmI CTRYAG 2 cut(s) 323, 1143
BfuI GTATCC 1 cut(s) 445
BglII AGATCT 1 cut(s) 727
BisI GCNGC 6 cut(s) 55, 305, 502, 795, 1134, 1167
BlsI GCNGC 6 cut(s) 56, 306, 503, 796, 1135, 1168
BmcAI AGTACT 2 cut(s) 550, 559
Bme1390I CCNGG 2 cut(s) 33, 345
Bme18I GGWCC 2 cut(s) 375, 706
BmgT120I GGNCC 2 cut(s) 375, 706
BmiI GGNNCC 3 cut(s) 442, 708, 1291
BmrFI CCNGG 2 cut(s) 33, 345
BmsI GCATC 3 cut(s) 619, 781, 811
BmtI GCTAGC 1 cut(s) 72
BoxI GACNNNNGTC 1 cut(s) 690
BpiI GAAGAC 1 cut(s) 88
BsaJI CCNNGG 3 cut(s) 32, 343, 581
BsaWI WCCGGW 3 cut(s) 372, 437, 703
Bsc4I CCNNNNNNNGG 1 cut(s) 1061
Bse118I RCCGGY 1 cut(s) 372
Bse1I ACTGG 4 cut(s) 216, 423, 568, 769
Bse3DI GCAATG 1 cut(s) 925
BseAI TCCGGA 2 cut(s) 437, 703
BseBI CCWGG 2 cut(s) 33, 345
BseDI CCNNGG 3 cut(s) 32, 343, 581
BseGI GGATG 3 cut(s) 634, 826, 1036
BseLI CCNNNNNNNGG 1 cut(s) 1061
BseMI GCAATG 1 cut(s) 925
BseNI ACTGG 4 cut(s) 216, 423, 568, 769
BseRI GAGGAG 6 cut(s) 502, 695, 983, 1025, 1208, 1243
BseXI GCAGC 4 cut(s) 66, 806, 1120, 1178
Bsh1236I CGCG 1 cut(s) 190
BshTI ACCGGT 1 cut(s) 372
BsiHKAI GWGCWC 1 cut(s) 580
BsiSI CCGG 3 cut(s) 373, 438, 704
BslFI GGGAC 1 cut(s) 247
BslI CCNNNNNNNGG 1 cut(s) 1061
BsmAI GTCTC 2 cut(s) 1190, 1259
BsmFI GGGAC 1 cut(s) 247
Bsp1286I GDGCHC 1 cut(s) 580
Bsp13I TCCGGA 2 cut(s) 437, 703
Bsp143I GATC 9 cut(s) 170, 290, 727, 874, 907, 928, 975, 1051, 1289
BspACI CCGC 4 cut(s) 177, 305, 501, 1136
BspEI TCCGGA 2 cut(s) 437, 703
BspFNI CGCG 1 cut(s) 190
BspHI TCATGA 6 cut(s) 235, 643, 733, 769, 910, 1042
BspLI GGNNCC 3 cut(s) 442, 708, 1291
BspMAI CTGCAG 1 cut(s) 1147
BspOI GCTAGC 1 cut(s) 72
BspPI GGATC 4 cut(s) 285, 1046, 1284, 1297
BsrDI GCAATG 1 cut(s) 925
BsrFI RCCGGY 1 cut(s) 372
BsrI ACTGG 4 cut(s) 216, 423, 568, 769
BssAI RCCGGY 1 cut(s) 372
BssECI CCNNGG 3 cut(s) 32, 343, 581
BssMI GATC 9 cut(s) 170, 290, 727, 874, 907, 928, 975, 1051, 1289
BssT1I CCWWGG 1 cut(s) 581
Bst2UI CCWGG 2 cut(s) 33, 345
Bst4CI ACNGT 3 cut(s) 692, 838, 1270
Bst6I CTCTTC 5 cut(s) 38, 343, 879, 978, 1220
BstAPI GCANNNNNTGC 1 cut(s) 1172
BstC8I GCNNGC 4 cut(s) 70, 181, 194, 462
BstEII GGTNACC 1 cut(s) 866
BstF5I GGATG 3 cut(s) 634, 826, 1036
BstFNI CGCG 1 cut(s) 190
BstHHI GCGC 1 cut(s) 192
BstKTI GATC 9 cut(s) 173, 293, 730, 877, 910, 931, 978, 1054, 1292
BstMAI GTCTC 2 cut(s) 1190, 1259
BstMBI GATC 9 cut(s) 170, 290, 727, 874, 907, 928, 975, 1051, 1289
BstMWI GCNNNNNNNGC 5 cut(s) 189, 310, 501, 1142, 1172
BstNI CCWGG 2 cut(s) 33, 345
BstNSI RCATGY 1 cut(s) 464
BstPAI GACNNNNGTC 1 cut(s) 690
BstPI GGTNACC 1 cut(s) 866
BstSCI CCNGG 2 cut(s) 31, 343
BstSFI CTRYAG 2 cut(s) 323, 1143
BstUI CGCG 1 cut(s) 190
BstV1I GCAGC 4 cut(s) 66, 806, 1120, 1178
BstV2I GAAGAC 1 cut(s) 88
BstX2I RGATCY 3 cut(s) 290, 727, 1289
BstYI RGATCY 3 cut(s) 290, 727, 1289
BsuI GTATCC 1 cut(s) 445
BtgZI GCGATG 1 cut(s) 171
BtsCI GGATG 3 cut(s) 634, 826, 1036
Cac8I GCNNGC 4 cut(s) 70, 181, 194, 462
CaiI CAGNNNCTG 2 cut(s) 764, 1172
CciI TCATGA 6 cut(s) 235, 643, 733, 769, 910, 1042
CfoI GCGC 1 cut(s) 192
Cfr10I RCCGGY 1 cut(s) 372
Cfr13I GGNCC 2 cut(s) 375, 706
CseI GACGC 1 cut(s) 371
Csp6I GTAC 4 cut(s) 24, 549, 558, 1140
CspAI ACCGGT 1 cut(s) 372
CviAII CATG 9 cut(s) 236, 461, 644, 734, 752, 770, 872, 911, 1043
CviQI GTAC 4 cut(s) 24, 549, 558, 1140
DpnI GATC 9 cut(s) 172, 292, 729, 876, 909, 930, 977, 1053, 1291
DpnII GATC 9 cut(s) 170, 290, 727, 874, 907, 928, 975, 1051, 1289
Eam1104I CTCTTC 5 cut(s) 38, 343, 879, 978, 1220
EarI CTCTTC 5 cut(s) 38, 343, 879, 978, 1220
Ecl136II GAGCTC 1 cut(s) 578
Eco130I CCWWGG 1 cut(s) 581
Eco24I GRGCYC 1 cut(s) 580
Eco47I GGWCC 2 cut(s) 375, 706
Eco53kI GAGCTC 1 cut(s) 578
Eco57I CTGAAG 3 cut(s) 300, 741, 741
Eco91I GGTNACC 1 cut(s) 866
EcoICRI GAGCTC 1 cut(s) 578
EcoO65I GGTNACC 1 cut(s) 866
EcoRII CCWGG 2 cut(s) 31, 343
EcoT14I CCWWGG 1 cut(s) 581
EcoT38I GRGCYC 1 cut(s) 580
ErhI CCWWGG 1 cut(s) 581
FaeI CATG 9 cut(s) 239, 464, 647, 737, 755, 773, 875, 914, 1046
FalI AAGNNNNNCTT 2 cut(s) 714, 746
FaqI GGGAC 1 cut(s) 247
FatI CATG 9 cut(s) 235, 460, 643, 733, 751, 769, 871, 910, 1042
FauI CCCGC 1 cut(s) 1129
FbaI TGATCA 2 cut(s) 874, 928
Fnu4HI GCNGC 6 cut(s) 55, 305, 502, 795, 1134, 1167
FokI GGATG 3 cut(s) 641, 833, 1043
FriOI GRGCYC 1 cut(s) 580
Fsp4HI GCNGC 6 cut(s) 55, 305, 502, 795, 1134, 1167
FspBI CTAG 5 cut(s) 69, 471, 480, 498, 555
GlaI GCGC 1 cut(s) 191
GluI GCNGC 6 cut(s) 55, 305, 502, 795, 1134, 1167
HapII CCGG 3 cut(s) 373, 438, 704
HgaI GACGC 1 cut(s) 371
HhaI GCGC 1 cut(s) 192
Hin1II CATG 9 cut(s) 239, 464, 647, 737, 755, 773, 875, 914, 1046
Hin6I GCGC 1 cut(s) 190
HinP1I GCGC 1 cut(s) 190
HindIII AAGCTT 2 cut(s) 1208, 1245
HinfI GANTC 6 cut(s) 239, 254, 586, 637, 803, 1283
HpaII CCGG 3 cut(s) 373, 438, 704
HphI GGTGA 3 cut(s) 54, 67, 860
Hpy188I TCNGA 2 cut(s) 244, 760
Hpy99I CGWCG 1 cut(s) 313
HpyAV CCTTC 7 cut(s) 210, 324, 352, 520, 710, 1055, 1153
HpyCH4III ACNGT 3 cut(s) 692, 838, 1270
HpyCH4V TGCA 5 cut(s) 464, 794, 1145, 1175, 1202
HpyF10VI GCNNNNNNNGC 5 cut(s) 189, 310, 501, 1142, 1172
Hsp92II CATG 9 cut(s) 239, 464, 647, 737, 755, 773, 875, 914, 1046
HspAI GCGC 1 cut(s) 190
Kpn2I TCCGGA 2 cut(s) 437, 703
Ksp22I TGATCA 2 cut(s) 874, 928
Kzo9I GATC 9 cut(s) 170, 290, 727, 874, 907, 928, 975, 1051, 1289
LmnI GCTCC 4 cut(s) 106, 415, 583, 1174
Lsp1109I GCAGC 4 cut(s) 66, 806, 1120, 1178
LweI GCATC 3 cut(s) 619, 781, 811
MaeI CTAG 5 cut(s) 69, 471, 480, 498, 555
MaeIII GTNAC 2 cut(s) 330, 866
MalI GATC 9 cut(s) 172, 292, 729, 876, 909, 930, 977, 1053, 1291
MboI GATC 9 cut(s) 170, 290, 727, 874, 907, 928, 975, 1051, 1289
MflI RGATCY 3 cut(s) 290, 727, 1289
MhlI GDGCHC 1 cut(s) 580
MluCI AATT 5 cut(s) 160, 603, 622, 713, 856
MlyI GAGTC 1 cut(s) 595
MmeI TCCRAC 1 cut(s) 631
MroI TCCGGA 2 cut(s) 437, 703
MroXI GAANNNNTTC 1 cut(s) 729
MseI TTAA 2 cut(s) 224, 786
MspI CCGG 3 cut(s) 373, 438, 704
MspR9I CCNGG 2 cut(s) 33, 345
MvaI CCWGG 2 cut(s) 33, 345
MvnI CGCG 1 cut(s) 190
MwoI GCNNNNNNNGC 5 cut(s) 189, 310, 501, 1142, 1172
NdeII GATC 9 cut(s) 170, 290, 727, 874, 907, 928, 975, 1051, 1289
NheI GCTAGC 1 cut(s) 68
NlaIII CATG 9 cut(s) 239, 464, 647, 737, 755, 773, 875, 914, 1046
NlaIV GGNNCC 3 cut(s) 442, 708, 1291
NmuCI GTSAC 2 cut(s) 330, 866
NspI RCATGY 1 cut(s) 464
PaeI GCATGC 1 cut(s) 464
PagI TCATGA 6 cut(s) 235, 643, 733, 769, 910, 1042
PdmI GAANNNNTTC 1 cut(s) 729
PfeI GAWTC 5 cut(s) 239, 254, 637, 803, 1283
PflFI GACNNNGTC 1 cut(s) 211
PinAI ACCGGT 1 cut(s) 372
PkrI GCNGC 6 cut(s) 56, 306, 503, 796, 1135, 1168
PleI GAGTC 1 cut(s) 594
PpsI GAGTC 1 cut(s) 594
PshAI GACNNNNGTC 1 cut(s) 690
Psp124BI GAGCTC 1 cut(s) 580
Psp6I CCWGG 2 cut(s) 31, 343
PspEI GGTNACC 1 cut(s) 866
PspGI CCWGG 2 cut(s) 31, 343
PspN4I GGNNCC 3 cut(s) 442, 708, 1291
PspPI GGNCC 2 cut(s) 375, 706
PstI CTGCAG 1 cut(s) 1147
PstNI CAGNNNCTG 2 cut(s) 764, 1172
PsuI RGATCY 3 cut(s) 290, 727, 1289
PsyI GACNNNGTC 1 cut(s) 211
RsaI GTAC 4 cut(s) 25, 550, 559, 1141
RsaNI GTAC 4 cut(s) 24, 549, 558, 1140
SacI GAGCTC 1 cut(s) 580
SaqAI TTAA 2 cut(s) 224, 786
SatI GCNGC 6 cut(s) 55, 305, 502, 795, 1134, 1167
Sau3AI GATC 9 cut(s) 170, 290, 727, 874, 907, 928, 975, 1051, 1289
Sau96I GGNCC 2 cut(s) 375, 706
ScaI AGTACT 2 cut(s) 550, 559
SchI GAGTC 1 cut(s) 595
ScrFI CCNGG 2 cut(s) 33, 345
SduI GDGCHC 1 cut(s) 580
SfaNI GCATC 3 cut(s) 619, 781, 811
SfcI CTRYAG 2 cut(s) 323, 1143
SinI GGWCC 2 cut(s) 375, 706
SpeI ACTAGT 3 cut(s) 470, 479, 554
SphI GCATGC 1 cut(s) 464
Sse9I AATT 5 cut(s) 160, 603, 622, 713, 856
SsiI CCGC 4 cut(s) 177, 305, 501, 1136
SspMI CTAG 5 cut(s) 69, 471, 480, 498, 555
SstI GAGCTC 1 cut(s) 580
StyD4I CCNGG 2 cut(s) 31, 343
StyI CCWWGG 1 cut(s) 581
TaaI ACNGT 3 cut(s) 692, 838, 1270
TasI AATT 5 cut(s) 160, 603, 622, 713, 856
TatI WGTACW 3 cut(s) 23, 548, 557
TauI GCSGC 2 cut(s) 307, 504
TfiI GAWTC 5 cut(s) 239, 254, 637, 803, 1283
Tru1I TTAA 2 cut(s) 224, 786
Tru9I TTAA 2 cut(s) 224, 786
TseFI GTSAC 2 cut(s) 330, 866
TseI GCWGC 4 cut(s) 54, 794, 1133, 1166
Tsp45I GTSAC 2 cut(s) 330, 866
TspDTI ATGAA 7 cut(s) 210, 722, 750, 846, 927, 1011, 1296
Tth111I GACNNNGTC 1 cut(s) 211
VpaK11BI GGWCC 2 cut(s) 375, 706
XapI RAATTY 2 cut(s) 603, 856
XceI RCATGY 1 cut(s) 464
XcmI CCANNNNNNNNNTGG 1 cut(s) 385
XmnI GAANNNNTTC 1 cut(s) 729
XspI CTAG 5 cut(s) 69, 471, 480, 498, 555
ZrmI AGTACT 2 cut(s) 550, 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.