Rh6AG448100

domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0005356
Physical Location & Seq
Forward (+)
63463068 .. 63466021
2954 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG448100.1

Sequence Viewer

Length: 1311 bp
ATGGCAACAAAACCAAAAAATAGTACTACTCCTGGGAGGGAGAGGAGAAGTAGCACATCACCAGCTAGCCTCACCAAAAGACCCACAAAACCAACTACTCCCGATAAATCTTCTTTAACCTCAAACTCATCATCAACAGCAAAACCAGTGCCAAACTATTTGAGATCAACCGCAAGCTCATCACGCCAGACTCCTTCTTTGACCAAGTCCAGGTCATTAAAGAAACATCATGTCTCTGATAATCAAGACTCCAACTCCCCAAAGCCTACACTAAATAGAGGAAGATCCTTTGACAAGCCACCATCACCTTCAGGACCACGGAAAATCATCTCCCCAGGAAGAGAGTCAAGAGACCCAGCACTTCGGTCTAAATCTTTTTCAGTTAAAAGCATCTCAAATGCTCCAAAACCCACCAGTAAATCTGTATCTGGAACCAAACCACAGGCTTCGTTTGCAAAGAGTAGTGGCATTAGTTTGAGGAGAAGCACTAGTGGTATCACTTCTGGTTCAAGTAATAAGGAAAGTAGAAGTAGTAGAAGTACTACTGGTAGTTCAAGAGCTCCGAGGAGTCATGACATAACACAAATTCTTAATCTTGAAGCTAATTTGGAAGCTTTAGATCATCATGAAGTTGGAGAGGTGGATAAGATTGAGAGTATTGAGGAGGACAACAGTCAAGACCTTCCGGACCCTAAATTAGCTGAAGAAGATCTTGATGATCAGCAGCAACAGCATGCTTCAGATACTGATCAAGCTAATGGTCATGACACTACTTCTGTTAAAGATGCAGCAGAGGAATCAAAAGCAAATGTGGATGTCAATAATGAAGAAGTTACTCAAGCAGAGAAAAATATTGATGGTCACCATGATCAAGTTGAAGAGGTTAATCACATTAGCAAAGATCATGAAAGCAATGACATTGATCACCACCCAGAAGAGGGTTCGGGTCTGACAAGCGAGGAAACAATAGCTGAGGAGGTGATCAAAGAAAAGAAAGAAGATGAAAACAAGAATGAGGTGGATATGGAAGTCGTTGATGGAGGAAGCAATGAGCTTGATCCAAAGGAAGGGCAAGATGGAGATATCAAAGAAGAAGCAAAGCCTGAGGAACCAGAGGTAACAGAAACGCCGCAAGCGGCTGCTGCTACTACAAGGAATAACAAGAAGGAGGCGGCTCCTGCATACAATGATGTGATTGAGGAGACTGCGAGTAAGCTGATGGCCCAAGAGAAGAGGAGGAACAAGGTCAAAGCTTTGGTTGGGGCTTTTGAGACTGTCATAGATCATGAATCAGGATCCAAATTAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaM_binding

Protein Analysis

436

Amino Acids

47.31

Weight (kDa)

5.46

Isoelectric Point (pI)

62.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaM_binding PF07839 325 - 427 1.1e-12 Plant calmodulin-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 685
AciI CCGC 4 cut(s) 171, 1130, 1136, 1172
AclWI GGATC 4 cut(s) 279, 1052, 1290, 1303
AcsI RAATTY 1 cut(s) 585
AcuI CTGAAG 3 cut(s) 294, 723, 723
AfaI GTAC 2 cut(s) 25, 541
AfiI CCNNNNNNNGG 4 cut(s) 210, 937, 938, 1067
AgsI TTSAA 4 cut(s) 510, 555, 599, 878
AhlI ACTAGT 1 cut(s) 488
AjnI CCWGG 3 cut(s) 31, 209, 334
Alw21I GWGCWC 1 cut(s) 562
Alw26I GTCTC 4 cut(s) 238, 345, 1196, 1265
AlwI GGATC 4 cut(s) 279, 1052, 1290, 1303
AlwNI CAGNNNCTG 1 cut(s) 746
Aor13HI TCCGGA 1 cut(s) 685
AoxI GGCC 1 cut(s) 1221
ApeKI GCWGC 4 cut(s) 724, 788, 1139, 1142
ApoI RAATTY 1 cut(s) 585
ArsI GACNNNNNNTTYG 2 cut(s) 181, 213
AspS9I GGNCC 3 cut(s) 314, 688, 1222
AsuHPI GGTGA 6 cut(s) 51, 64, 297, 854, 917, 991
AsuNHI GCTAGC 1 cut(s) 65
AvaII GGWCC 2 cut(s) 314, 688
AxyI CCTNAGG 1 cut(s) 1104
BamHI GGATCC 1 cut(s) 1295
BanII GRGCYC 1 cut(s) 562
Bbv12I GWGCWC 1 cut(s) 562
BbvCI CCTCAGC 1 cut(s) 972
BbvI GCAGC 4 cut(s) 736, 800, 1126, 1129
BccI CCATC 5 cut(s) 310, 851, 1031, 1070, 1213
BciT130I CCWGG 3 cut(s) 33, 211, 336
BclI TGATCA 5 cut(s) 718, 748, 868, 922, 981
BcoDI GTCTC 4 cut(s) 238, 345, 1196, 1265
BcuI ACTAGT 1 cut(s) 488
BfaI CTAG 2 cut(s) 66, 489
BglII AGATCT 1 cut(s) 709
BisI GCNGC 7 cut(s) 725, 789, 1130, 1137, 1140, 1143, 1173
BlsI GCNGC 7 cut(s) 726, 790, 1131, 1138, 1141, 1144, 1174
BmcAI AGTACT 2 cut(s) 25, 541
Bme1390I CCNGG 3 cut(s) 33, 211, 336
Bme18I GGWCC 2 cut(s) 314, 688
BmgT120I GGNCC 3 cut(s) 314, 688, 1222
BmiI GGNNCC 5 cut(s) 433, 690, 1110, 1176, 1297
BmrFI CCNGG 3 cut(s) 33, 211, 336
BmsI GCATC 2 cut(s) 399, 775
BmtI GCTAGC 1 cut(s) 69
BoxI GACNNNNGTC 1 cut(s) 672
Bpu10I CCTNAGC 1 cut(s) 972
BpuEI CTTGAG 1 cut(s) 822
BsaBI GATNNNNATC 1 cut(s) 747
BsaI GGTCTC 1 cut(s) 345
BsaJI CCNNGG 4 cut(s) 32, 317, 334, 563
BsaWI WCCGGW 1 cut(s) 685
BsaXI ACNNNNNCTCC 2 cut(s) 239, 269
Bsc4I CCNNNNNNNGG 4 cut(s) 210, 937, 938, 1067
Bse1I ACTGG 3 cut(s) 146, 414, 550
Bse21I CCTNAGG 1 cut(s) 1104
Bse3DI GCAATG 2 cut(s) 919, 1054
Bse8I GATNNNNATC 1 cut(s) 747
BseAI TCCGGA 1 cut(s) 685
BseBI CCWGG 3 cut(s) 33, 211, 336
BseDI CCNNGG 4 cut(s) 32, 317, 334, 563
BseGI GGATG 1 cut(s) 820
BseJI GATNNNNATC 1 cut(s) 747
BseLI CCNNNNNNNGG 4 cut(s) 210, 937, 938, 1067
BseMI GCAATG 2 cut(s) 919, 1054
BseMII CTCAG 2 cut(s) 963, 1095
BseNI ACTGG 3 cut(s) 146, 414, 550
BseRI GAGGAG 7 cut(s) 58, 493, 580, 677, 989, 1214, 1249
BseXI GCAGC 4 cut(s) 736, 800, 1126, 1129
BseYI CCCAGC 1 cut(s) 355
BshFI GGCC 1 cut(s) 1223
BsiHKAI GWGCWC 1 cut(s) 562
BsiSI CCGG 1 cut(s) 686
BslI CCNNNNNNNGG 4 cut(s) 210, 937, 938, 1067
BsmAI GTCTC 4 cut(s) 238, 345, 1196, 1265
BsnI GGCC 1 cut(s) 1223
Bso31I GGTCTC 1 cut(s) 345
Bsp1286I GDGCHC 1 cut(s) 562
Bsp13I TCCGGA 1 cut(s) 685
BspACI CCGC 4 cut(s) 171, 1130, 1136, 1172
BspANI GGCC 1 cut(s) 1223
BspCNI CTCAG 2 cut(s) 964, 1096
BspEI TCCGGA 1 cut(s) 685
BspHI TCATGA 5 cut(s) 571, 625, 763, 904, 1285
BspLI GGNNCC 5 cut(s) 433, 690, 1110, 1176, 1297
BspOI GCTAGC 1 cut(s) 69
BspPI GGATC 4 cut(s) 279, 1052, 1290, 1303
BspTNI GGTCTC 1 cut(s) 345
BsrDI GCAATG 2 cut(s) 919, 1054
BsrI ACTGG 3 cut(s) 146, 414, 550
BssECI CCNNGG 4 cut(s) 32, 317, 334, 563
Bst2UI CCWGG 3 cut(s) 33, 211, 336
Bst4CI ACNGT 2 cut(s) 674, 1276
Bst6I CTCTTC 4 cut(s) 334, 873, 930, 1226
BstC8I GCNNGC 4 cut(s) 67, 175, 735, 1134
BstDEI CTNAG 2 cut(s) 972, 1104
BstDSI CCRYGG 1 cut(s) 317
BstEII GGTNACC 1 cut(s) 860
BstF5I GGATG 1 cut(s) 820
BstMAI GTCTC 4 cut(s) 238, 345, 1196, 1265
BstMWI GCNNNNNNNGC 5 cut(s) 183, 452, 730, 1142, 1178
BstNI CCWGG 3 cut(s) 33, 211, 336
BstNSI RCATGY 1 cut(s) 737
BstPAI GACNNNNGTC 1 cut(s) 672
BstPI GGTNACC 1 cut(s) 860
BstSCI CCNGG 3 cut(s) 31, 209, 334
BstV1I GCAGC 4 cut(s) 736, 800, 1126, 1129
BstX2I RGATCY 3 cut(s) 284, 709, 1295
BstYI RGATCY 3 cut(s) 284, 709, 1295
Bsu36I CCTNAGG 1 cut(s) 1104
BsuRI GGCC 1 cut(s) 1223
BtgI CCRYGG 1 cut(s) 317
BtsCI GGATG 1 cut(s) 820
BtsIMutI CAGTG 1 cut(s) 153
Cac8I GCNNGC 4 cut(s) 67, 175, 735, 1134
CaiI CAGNNNCTG 1 cut(s) 746
CciI TCATGA 5 cut(s) 571, 625, 763, 904, 1285
Cfr13I GGNCC 3 cut(s) 314, 688, 1222
Csp6I GTAC 2 cut(s) 24, 540
CviAII CATG 8 cut(s) 230, 572, 626, 734, 764, 866, 905, 1286
CviQI GTAC 2 cut(s) 24, 540
DdeI CTNAG 2 cut(s) 972, 1104
Eam1104I CTCTTC 4 cut(s) 334, 873, 930, 1226
EarI CTCTTC 4 cut(s) 334, 873, 930, 1226
Ecl136II GAGCTC 1 cut(s) 560
Eco24I GRGCYC 1 cut(s) 562
Eco31I GGTCTC 1 cut(s) 345
Eco32I GATATC 1 cut(s) 1084
Eco47I GGWCC 2 cut(s) 314, 688
Eco53kI GAGCTC 1 cut(s) 560
Eco57I CTGAAG 3 cut(s) 294, 723, 723
Eco81I CCTNAGG 1 cut(s) 1104
Eco91I GGTNACC 1 cut(s) 860
EcoICRI GAGCTC 1 cut(s) 560
EcoO65I GGTNACC 1 cut(s) 860
EcoRII CCWGG 3 cut(s) 31, 209, 334
EcoRV GATATC 1 cut(s) 1084
EcoT38I GRGCYC 1 cut(s) 562
FaeI CATG 8 cut(s) 233, 575, 629, 737, 767, 869, 908, 1289
FalI AAGNNNNNCTT 2 cut(s) 696, 728
FatI CATG 8 cut(s) 229, 571, 625, 733, 763, 865, 904, 1285
FbaI TGATCA 5 cut(s) 718, 748, 868, 922, 981
Fnu4HI GCNGC 7 cut(s) 725, 789, 1130, 1137, 1140, 1143, 1173
FokI GGATG 1 cut(s) 827
FriOI GRGCYC 1 cut(s) 562
Fsp4HI GCNGC 7 cut(s) 725, 789, 1130, 1137, 1140, 1143, 1173
FspBI CTAG 2 cut(s) 66, 489
GluI GCNGC 7 cut(s) 725, 789, 1130, 1137, 1140, 1143, 1173
GsaI CCCAGC 1 cut(s) 359
HaeIII GGCC 1 cut(s) 1223
HapII CCGG 1 cut(s) 686
Hin1II CATG 8 cut(s) 233, 575, 629, 737, 767, 869, 908, 1289
HindIII AAGCTT 2 cut(s) 612, 1251
HinfI GANTC 6 cut(s) 190, 248, 344, 568, 797, 1289
HpaII CCGG 1 cut(s) 686
HphI GGTGA 6 cut(s) 51, 64, 297, 854, 917, 991
Hpy188I TCNGA 4 cut(s) 238, 564, 742, 951
HpyAV CCTTC 5 cut(s) 204, 318, 692, 1061, 1159
HpyCH4III ACNGT 2 cut(s) 674, 1276
HpyCH4V TGCA 3 cut(s) 455, 788, 1181
HpyF10VI GCNNNNNNNGC 5 cut(s) 183, 452, 730, 1142, 1178
HpyF3I CTNAG 2 cut(s) 972, 1104
Hsp92II CATG 8 cut(s) 233, 575, 629, 737, 767, 869, 908, 1289
Kpn2I TCCGGA 1 cut(s) 685
Ksp22I TGATCA 5 cut(s) 718, 748, 868, 922, 981
LmnI GCTCC 3 cut(s) 406, 565, 1180
Lsp1109I GCAGC 4 cut(s) 736, 800, 1126, 1129
LweI GCATC 2 cut(s) 399, 775
MaeI CTAG 2 cut(s) 66, 489
MaeIII GTNAC 3 cut(s) 832, 860, 1117
MflI RGATCY 3 cut(s) 284, 709, 1295
MhlI GDGCHC 1 cut(s) 562
MluCI AATT 5 cut(s) 585, 604, 695, 1301, 1306
MlyI GAGTC 4 cut(s) 184, 242, 353, 577
MmeI TCCRAC 2 cut(s) 276, 613
MroI TCCGGA 1 cut(s) 685
MseI TTAA 8 cut(s) 116, 218, 384, 591, 780, 885, 1304, 1309
MspI CCGG 1 cut(s) 686
MspR9I CCNGG 3 cut(s) 33, 211, 336
MvaI CCWGG 3 cut(s) 33, 211, 336
MwoI GCNNNNNNNGC 5 cut(s) 183, 452, 730, 1142, 1178
NheI GCTAGC 1 cut(s) 65
NlaIII CATG 8 cut(s) 233, 575, 629, 737, 767, 869, 908, 1289
NlaIV GGNNCC 5 cut(s) 433, 690, 1110, 1176, 1297
NmuCI GTSAC 1 cut(s) 860
NspI RCATGY 1 cut(s) 737
PaeI GCATGC 1 cut(s) 737
PagI TCATGA 5 cut(s) 571, 625, 763, 904, 1285
PfeI GAWTC 2 cut(s) 797, 1289
PflFI GACNNNGTC 1 cut(s) 205
PkrI GCNGC 7 cut(s) 726, 790, 1131, 1138, 1141, 1144, 1174
PleI GAGTC 4 cut(s) 184, 242, 352, 576
PpsI GAGTC 4 cut(s) 184, 242, 352, 576
PshAI GACNNNNGTC 1 cut(s) 672
Psp124BI GAGCTC 1 cut(s) 562
Psp6I CCWGG 3 cut(s) 31, 209, 334
PspEI GGTNACC 1 cut(s) 860
PspFI CCCAGC 1 cut(s) 355
PspGI CCWGG 3 cut(s) 31, 209, 334
PspN4I GGNNCC 5 cut(s) 433, 690, 1110, 1176, 1297
PspPI GGNCC 3 cut(s) 314, 688, 1222
PstNI CAGNNNCTG 1 cut(s) 746
PsuI RGATCY 3 cut(s) 284, 709, 1295
PsyI GACNNNGTC 1 cut(s) 205
RsaI GTAC 2 cut(s) 25, 541
RsaNI GTAC 2 cut(s) 24, 540
SacI GAGCTC 1 cut(s) 562
SaqAI TTAA 8 cut(s) 116, 218, 384, 591, 780, 885, 1304, 1309
SatI GCNGC 7 cut(s) 725, 789, 1130, 1137, 1140, 1143, 1173
Sau96I GGNCC 3 cut(s) 314, 688, 1222
ScaI AGTACT 2 cut(s) 25, 541
SchI GAGTC 4 cut(s) 184, 242, 353, 577
ScrFI CCNGG 3 cut(s) 33, 211, 336
SduI GDGCHC 1 cut(s) 562
SfaNI GCATC 2 cut(s) 399, 775
SinI GGWCC 2 cut(s) 314, 688
SmlI CTYRAG 1 cut(s) 837
SmoI CTYRAG 1 cut(s) 837
SpeI ACTAGT 1 cut(s) 488
SphI GCATGC 1 cut(s) 737
Sse9I AATT 5 cut(s) 585, 604, 695, 1301, 1306
SsiI CCGC 4 cut(s) 171, 1130, 1136, 1172
SspI AATATT 1 cut(s) 853
SspMI CTAG 2 cut(s) 66, 489
SstI GAGCTC 1 cut(s) 562
StyD4I CCNGG 3 cut(s) 31, 209, 334
TaaI ACNGT 2 cut(s) 674, 1276
TaqII GACCGA 1 cut(s) 354
TasI AATT 5 cut(s) 585, 604, 695, 1301, 1306
TatI WGTACW 2 cut(s) 23, 539
TauI GCSGC 3 cut(s) 1132, 1139, 1175
TfiI GAWTC 2 cut(s) 797, 1289
Tru1I TTAA 8 cut(s) 116, 218, 384, 591, 780, 885, 1304, 1309
Tru9I TTAA 8 cut(s) 116, 218, 384, 591, 780, 885, 1304, 1309
TscAI CASTG 1 cut(s) 153
TseFI GTSAC 1 cut(s) 860
TseI GCWGC 4 cut(s) 724, 788, 1139, 1142
Tsp45I GTSAC 1 cut(s) 860
TspDTI ATGAA 5 cut(s) 642, 840, 921, 1017, 1302
TspGWI ACGGA 1 cut(s) 334
TspRI CASTG 1 cut(s) 153
Tth111I GACNNNGTC 1 cut(s) 205
VpaK11BI GGWCC 2 cut(s) 314, 688
XapI RAATTY 1 cut(s) 585
XceI RCATGY 1 cut(s) 737
XspI CTAG 2 cut(s) 66, 489
ZrmI AGTACT 2 cut(s) 25, 541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.