Rh6BG452700

domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0005356
Physical Location & Seq
Reverse (-)
66652241 .. 66657456
5216 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG452700.1

Sequence Viewer

Length: 1317 bp
ATGGCAACAAAACCAAAAAATAGTACTACTCCTGGGAGGGAGAGGAGAAGTAGCACATCACCAGCTAGCCTCACCAAAAGACCCACAAAACCAACTACTCCTGACAAACCTTCTTCAACCTCAAACTCATCATCAACAGCAAAACCAGTGCCAAACTATTTGAGATCAACCGCAAGCTCATCACGCCAGACTCCTTCTTTGACCAAGTCCAGGTCATTAAAGAAACATCATGTCTCTGATAATCAAGACTCCAACTCCCCAAAGCCTACACTAAATAGAAGAAGATCCTTTGACAAGCCACCATCACCTTCAGGACCACGGAAAATCATCTCCCCAGGAAGAGAGTCAAGAGACCCAGCACTTCGATCTAAATCTTTTTCAGTTAAAAGCATCTCAAATGCTCCAAAACCCACCAGTAAATCTGTATCTGTATCTGGAACCAAACCACAGGCTTCGTTTGCAAAGAGTAGTGGCACTAGTTTGAGGAGAAGCACTAGTGGTATCACTTCTGGTTCAAGTAAGAAGGAAAGTAGAAGTAGTAGAAGTACTACTGGTAGTTCAAGAGCTCCGAGGAGTCATGACATAACACAAATTCTTAATCTTGAAACTAATTTGGAAGCTTTAGATCATCATGAAGTTGGAGAGGTGGATAAGATTGAGAGTATTGAGGAGGACAACACTCAAGACCTTCCGGACCCTAAATTAGCTGAAGAAGATCTTGATGATCAGCAGCAACATCATGCTTCAGATAGTGATCAAGCTAATGGTCATGACACTACTTCTGTTAAAGATGCAGCAGAGGAATCAAAAGCAAATGTGGATGTCAATAATGAAGAAGTTACTCAAGCAGAGAAAAATATTGATGGTCACTATGATCAAGTTGAAGAGGTTAATCACATTAGCAAAGATCATGAAAGCAATGACATTGATCACCACCCAGAAGAGGGTTCGGGTCTGACAAGCGAGGAAACAATAGCTGAGGAGGTGATCAAAGAAAAGAAAGAAGATGAAAACAAGAATGAGGTGGATATGGAAGTCGTTGATGGAGGAAGCAATGAGCTTGATCCAAAGGAAGGGCAAGATGGAGATATCAAAGAAGAAGCAAAGCCTGAGGAACCAGAGGTAACAGAAACGCCGCAAGCGGCTGCTGCTACTACAGGGAATAACAAGAAGGAGGCGGCTCCTGCATACAATGATGTGATTGAGGAGACTGCGAGTAAGCTGATGGCCCAAGAGAAGAGGAGGAACAAGGTCAAAGCTTTGGTTGGGGCTTTTGAGACTGTCATAGATCATGAATCAGGATCCAAATTAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaM_binding

Protein Analysis

438

Amino Acids

47.55

Weight (kDa)

5.52

Isoelectric Point (pI)

64.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaM_binding PF07839 327 - 429 5.6e-13 Plant calmodulin-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 691
AciI CCGC 4 cut(s) 171, 1136, 1142, 1178
AclWI GGATC 4 cut(s) 279, 1058, 1296, 1309
AcsI RAATTY 1 cut(s) 591
AcuI CTGAAG 3 cut(s) 294, 729, 729
AfaI GTAC 2 cut(s) 25, 547
AfiI CCNNNNNNNGG 4 cut(s) 210, 943, 944, 1073
AgsI TTSAA 5 cut(s) 117, 516, 561, 605, 884
AhlI ACTAGT 2 cut(s) 476, 494
AjnI CCWGG 3 cut(s) 31, 209, 334
Alw21I GWGCWC 1 cut(s) 568
Alw26I GTCTC 4 cut(s) 238, 345, 1202, 1271
AlwI GGATC 4 cut(s) 279, 1058, 1296, 1309
Aor13HI TCCGGA 1 cut(s) 691
AoxI GGCC 1 cut(s) 1227
ApeKI GCWGC 4 cut(s) 730, 794, 1145, 1148
ApoI RAATTY 1 cut(s) 591
ArsI GACNNNNNNTTYG 2 cut(s) 181, 213
AspS9I GGNCC 3 cut(s) 314, 694, 1228
AsuHPI GGTGA 5 cut(s) 51, 64, 297, 923, 997
AsuNHI GCTAGC 1 cut(s) 65
AvaII GGWCC 2 cut(s) 314, 694
AxyI CCTNAGG 1 cut(s) 1110
BamHI GGATCC 1 cut(s) 1301
BanII GRGCYC 1 cut(s) 568
Bbv12I GWGCWC 1 cut(s) 568
BbvCI CCTCAGC 1 cut(s) 978
BbvI GCAGC 4 cut(s) 742, 806, 1132, 1135
BccI CCATC 5 cut(s) 310, 857, 1037, 1076, 1219
BciT130I CCWGG 3 cut(s) 33, 211, 336
BclI TGATCA 5 cut(s) 724, 754, 874, 928, 987
BcoDI GTCTC 4 cut(s) 238, 345, 1202, 1271
BcuI ACTAGT 2 cut(s) 476, 494
BfaI CTAG 3 cut(s) 66, 477, 495
BfmI CTRYAG 1 cut(s) 1155
BglII AGATCT 1 cut(s) 715
BisI GCNGC 7 cut(s) 731, 795, 1136, 1143, 1146, 1149, 1179
BlsI GCNGC 7 cut(s) 732, 796, 1137, 1144, 1147, 1150, 1180
BmcAI AGTACT 2 cut(s) 25, 547
Bme1390I CCNGG 3 cut(s) 33, 211, 336
Bme18I GGWCC 2 cut(s) 314, 694
BmgT120I GGNCC 3 cut(s) 314, 694, 1228
BmiI GGNNCC 5 cut(s) 439, 696, 1116, 1182, 1303
BmrFI CCNGG 3 cut(s) 33, 211, 336
BmsI GCATC 2 cut(s) 399, 781
BmtI GCTAGC 1 cut(s) 69
Bpu10I CCTNAGC 1 cut(s) 978
BpuEI CTTGAG 2 cut(s) 666, 828
BsaBI GATNNNNATC 2 cut(s) 370, 753
BsaI GGTCTC 1 cut(s) 345
BsaJI CCNNGG 4 cut(s) 32, 317, 334, 569
BsaWI WCCGGW 1 cut(s) 691
BsaXI ACNNNNNCTCC 2 cut(s) 239, 269
Bsc4I CCNNNNNNNGG 4 cut(s) 210, 943, 944, 1073
Bse1I ACTGG 3 cut(s) 146, 414, 556
Bse21I CCTNAGG 1 cut(s) 1110
Bse3DI GCAATG 2 cut(s) 925, 1060
Bse8I GATNNNNATC 2 cut(s) 370, 753
BseAI TCCGGA 1 cut(s) 691
BseBI CCWGG 3 cut(s) 33, 211, 336
BseDI CCNNGG 4 cut(s) 32, 317, 334, 569
BseGI GGATG 1 cut(s) 826
BseJI GATNNNNATC 2 cut(s) 370, 753
BseLI CCNNNNNNNGG 4 cut(s) 210, 943, 944, 1073
BseMI GCAATG 2 cut(s) 925, 1060
BseMII CTCAG 2 cut(s) 969, 1101
BseNI ACTGG 3 cut(s) 146, 414, 556
BseRI GAGGAG 7 cut(s) 58, 499, 586, 683, 995, 1220, 1255
BseXI GCAGC 4 cut(s) 742, 806, 1132, 1135
BseYI CCCAGC 1 cut(s) 355
BshFI GGCC 1 cut(s) 1229
BsiHKAI GWGCWC 1 cut(s) 568
BsiSI CCGG 1 cut(s) 692
BslI CCNNNNNNNGG 4 cut(s) 210, 943, 944, 1073
BsmAI GTCTC 4 cut(s) 238, 345, 1202, 1271
BsnI GGCC 1 cut(s) 1229
Bso31I GGTCTC 1 cut(s) 345
Bsp1286I GDGCHC 1 cut(s) 568
Bsp13I TCCGGA 1 cut(s) 691
BspACI CCGC 4 cut(s) 171, 1136, 1142, 1178
BspANI GGCC 1 cut(s) 1229
BspCNI CTCAG 2 cut(s) 970, 1102
BspEI TCCGGA 1 cut(s) 691
BspHI TCATGA 5 cut(s) 577, 631, 769, 910, 1291
BspLI GGNNCC 5 cut(s) 439, 696, 1116, 1182, 1303
BspOI GCTAGC 1 cut(s) 69
BspPI GGATC 4 cut(s) 279, 1058, 1296, 1309
BspTNI GGTCTC 1 cut(s) 345
BsrDI GCAATG 2 cut(s) 925, 1060
BsrI ACTGG 3 cut(s) 146, 414, 556
BssECI CCNNGG 4 cut(s) 32, 317, 334, 569
Bst2UI CCWGG 3 cut(s) 33, 211, 336
Bst4CI ACNGT 1 cut(s) 1282
Bst6I CTCTTC 4 cut(s) 334, 879, 936, 1232
BstC8I GCNNGC 3 cut(s) 67, 175, 1140
BstDEI CTNAG 2 cut(s) 978, 1110
BstDSI CCRYGG 1 cut(s) 317
BstF5I GGATG 1 cut(s) 826
BstMAI GTCTC 4 cut(s) 238, 345, 1202, 1271
BstMWI GCNNNNNNNGC 4 cut(s) 183, 458, 1148, 1184
BstNI CCWGG 3 cut(s) 33, 211, 336
BstSCI CCNGG 3 cut(s) 31, 209, 334
BstSFI CTRYAG 1 cut(s) 1155
BstV1I GCAGC 4 cut(s) 742, 806, 1132, 1135
BstX2I RGATCY 3 cut(s) 284, 715, 1301
BstYI RGATCY 3 cut(s) 284, 715, 1301
Bsu36I CCTNAGG 1 cut(s) 1110
BsuRI GGCC 1 cut(s) 1229
BtgI CCRYGG 1 cut(s) 317
BtsCI GGATG 1 cut(s) 826
BtsIMutI CAGTG 1 cut(s) 153
Cac8I GCNNGC 3 cut(s) 67, 175, 1140
CciI TCATGA 5 cut(s) 577, 631, 769, 910, 1291
Cfr13I GGNCC 3 cut(s) 314, 694, 1228
Csp6I GTAC 2 cut(s) 24, 546
CviAII CATG 7 cut(s) 230, 578, 632, 740, 770, 911, 1292
CviQI GTAC 2 cut(s) 24, 546
DdeI CTNAG 2 cut(s) 978, 1110
Eam1104I CTCTTC 4 cut(s) 334, 879, 936, 1232
EarI CTCTTC 4 cut(s) 334, 879, 936, 1232
Ecl136II GAGCTC 1 cut(s) 566
Eco24I GRGCYC 1 cut(s) 568
Eco31I GGTCTC 1 cut(s) 345
Eco32I GATATC 1 cut(s) 1090
Eco47I GGWCC 2 cut(s) 314, 694
Eco53kI GAGCTC 1 cut(s) 566
Eco57I CTGAAG 3 cut(s) 294, 729, 729
Eco81I CCTNAGG 1 cut(s) 1110
EcoICRI GAGCTC 1 cut(s) 566
EcoRII CCWGG 3 cut(s) 31, 209, 334
EcoRV GATATC 1 cut(s) 1090
EcoT38I GRGCYC 1 cut(s) 568
FaeI CATG 7 cut(s) 233, 581, 635, 743, 773, 914, 1295
FalI AAGNNNNNCTT 2 cut(s) 702, 734
FatI CATG 7 cut(s) 229, 577, 631, 739, 769, 910, 1291
FbaI TGATCA 5 cut(s) 724, 754, 874, 928, 987
Fnu4HI GCNGC 7 cut(s) 731, 795, 1136, 1143, 1146, 1149, 1179
FokI GGATG 1 cut(s) 833
FriOI GRGCYC 1 cut(s) 568
Fsp4HI GCNGC 7 cut(s) 731, 795, 1136, 1143, 1146, 1149, 1179
FspBI CTAG 3 cut(s) 66, 477, 495
GluI GCNGC 7 cut(s) 731, 795, 1136, 1143, 1146, 1149, 1179
GsaI CCCAGC 1 cut(s) 359
HaeIII GGCC 1 cut(s) 1229
HapII CCGG 1 cut(s) 692
Hin1II CATG 7 cut(s) 233, 581, 635, 743, 773, 914, 1295
HindIII AAGCTT 2 cut(s) 618, 1257
HinfI GANTC 6 cut(s) 190, 248, 344, 574, 803, 1295
HpaII CCGG 1 cut(s) 692
HphI GGTGA 5 cut(s) 51, 64, 297, 923, 997
Hpy188I TCNGA 4 cut(s) 238, 570, 748, 957
HpyAV CCTTC 7 cut(s) 120, 204, 318, 517, 698, 1067, 1165
HpyCH4III ACNGT 1 cut(s) 1282
HpyCH4V TGCA 3 cut(s) 461, 794, 1187
HpyF10VI GCNNNNNNNGC 4 cut(s) 183, 458, 1148, 1184
HpyF3I CTNAG 2 cut(s) 978, 1110
Hsp92II CATG 7 cut(s) 233, 581, 635, 743, 773, 914, 1295
Kpn2I TCCGGA 1 cut(s) 691
Ksp22I TGATCA 5 cut(s) 724, 754, 874, 928, 987
LmnI GCTCC 3 cut(s) 406, 571, 1186
Lsp1109I GCAGC 4 cut(s) 742, 806, 1132, 1135
LweI GCATC 2 cut(s) 399, 781
MaeI CTAG 3 cut(s) 66, 477, 495
MaeIII GTNAC 3 cut(s) 838, 866, 1123
MflI RGATCY 3 cut(s) 284, 715, 1301
MhlI GDGCHC 1 cut(s) 568
MluCI AATT 5 cut(s) 591, 610, 701, 1307, 1312
MlyI GAGTC 4 cut(s) 184, 242, 353, 583
MmeI TCCRAC 2 cut(s) 276, 619
MroI TCCGGA 1 cut(s) 691
MseI TTAA 7 cut(s) 218, 384, 597, 786, 891, 1310, 1315
MspI CCGG 1 cut(s) 692
MspR9I CCNGG 3 cut(s) 33, 211, 336
MvaI CCWGG 3 cut(s) 33, 211, 336
MwoI GCNNNNNNNGC 4 cut(s) 183, 458, 1148, 1184
NheI GCTAGC 1 cut(s) 65
NlaIII CATG 7 cut(s) 233, 581, 635, 743, 773, 914, 1295
NlaIV GGNNCC 5 cut(s) 439, 696, 1116, 1182, 1303
NmuCI GTSAC 1 cut(s) 866
PagI TCATGA 5 cut(s) 577, 631, 769, 910, 1291
PfeI GAWTC 2 cut(s) 803, 1295
PflFI GACNNNGTC 1 cut(s) 205
PkrI GCNGC 7 cut(s) 732, 796, 1137, 1144, 1147, 1150, 1180
PleI GAGTC 4 cut(s) 184, 242, 352, 582
PpsI GAGTC 4 cut(s) 184, 242, 352, 582
Psp124BI GAGCTC 1 cut(s) 568
Psp6I CCWGG 3 cut(s) 31, 209, 334
PspFI CCCAGC 1 cut(s) 355
PspGI CCWGG 3 cut(s) 31, 209, 334
PspN4I GGNNCC 5 cut(s) 439, 696, 1116, 1182, 1303
PspPI GGNCC 3 cut(s) 314, 694, 1228
PsuI RGATCY 3 cut(s) 284, 715, 1301
PsyI GACNNNGTC 1 cut(s) 205
RsaI GTAC 2 cut(s) 25, 547
RsaNI GTAC 2 cut(s) 24, 546
SacI GAGCTC 1 cut(s) 568
SaqAI TTAA 7 cut(s) 218, 384, 597, 786, 891, 1310, 1315
SatI GCNGC 7 cut(s) 731, 795, 1136, 1143, 1146, 1149, 1179
Sau96I GGNCC 3 cut(s) 314, 694, 1228
ScaI AGTACT 2 cut(s) 25, 547
SchI GAGTC 4 cut(s) 184, 242, 353, 583
ScrFI CCNGG 3 cut(s) 33, 211, 336
SduI GDGCHC 1 cut(s) 568
SfaNI GCATC 2 cut(s) 399, 781
SfcI CTRYAG 1 cut(s) 1155
SinI GGWCC 2 cut(s) 314, 694
SmlI CTYRAG 2 cut(s) 681, 843
SmoI CTYRAG 2 cut(s) 681, 843
SpeI ACTAGT 2 cut(s) 476, 494
Sse9I AATT 5 cut(s) 591, 610, 701, 1307, 1312
SsiI CCGC 4 cut(s) 171, 1136, 1142, 1178
SspI AATATT 1 cut(s) 859
SspMI CTAG 3 cut(s) 66, 477, 495
SstI GAGCTC 1 cut(s) 568
StyD4I CCNGG 3 cut(s) 31, 209, 334
TaaI ACNGT 1 cut(s) 1282
TaqI TCGA 1 cut(s) 364
TasI AATT 5 cut(s) 591, 610, 701, 1307, 1312
TatI WGTACW 2 cut(s) 23, 545
TauI GCSGC 3 cut(s) 1138, 1145, 1181
TfiI GAWTC 2 cut(s) 803, 1295
Tru1I TTAA 7 cut(s) 218, 384, 597, 786, 891, 1310, 1315
Tru9I TTAA 7 cut(s) 218, 384, 597, 786, 891, 1310, 1315
TscAI CASTG 1 cut(s) 153
TseFI GTSAC 1 cut(s) 866
TseI GCWGC 4 cut(s) 730, 794, 1145, 1148
Tsp45I GTSAC 1 cut(s) 866
TspDTI ATGAA 5 cut(s) 648, 846, 927, 1023, 1308
TspGWI ACGGA 1 cut(s) 334
TspRI CASTG 1 cut(s) 153
Tth111I GACNNNGTC 1 cut(s) 205
VpaK11BI GGWCC 2 cut(s) 314, 694
XapI RAATTY 1 cut(s) 591
XspI CTAG 3 cut(s) 66, 477, 495
ZrmI AGTACT 2 cut(s) 25, 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.