Rw6G039020

domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0005356
Physical Location & Seq
Forward (+)
63082962 .. 63086038
3077 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G039020.1

Sequence Viewer

Length: 1302 bp
ATGGCAACAAAACCAAAAAATAGTACTACTCCTGGGAGGGAGAGGAGAAGTAGCACATCACCAGCGAGCCTCACCAAAAGACCCACAAAACCAACTACTCCTGATAAATCTTCTTCAACCTCAAACTCATCATCGACAGCAAAACCAGTGCCAAACTATTTGAGATCAACCGCAAGCTCATCACGCCAGACTCCTTCTTTGACCAAGTCCAGGTCATTAAAGAAACATCATGTCTCTGATAATCAAGACTCCAACTCCCCAAAGCCTACACTAAATAGAAGAAGATCCTTTGACAAGCCACCATCACCTTCAGGACCACGGAAAATCATCTCCCCAGGAAGAGAGTCAAGAGACCCAGCACTTCGGTCTAAATCTTTTTCAGTTAAAAGCATCTCAAATGCTCCAAAACCCACCAGTAAATCTGTATCTGGAACCAAACCACAGGCTTCGTTTGCAAAGAGTAGTGGCACTAGTTTGAGGAGAAGCACTAGTGGTATCACTTCTGGTTCAAGTAAGAAGGAAAGTAGAGGTACTACTGGTAGTTCAAGAGCTCCGAGGAGTCGTGACATAACACAAATTCTTAATCTTGAAGCTAATTTGGAAGCTTTAGATCATCATGAAGTTGGAGAGGTGGATAAGATTGAGAGTATTGAGGAGGACAACAGTCAAGACCTTCCGGACCCTAAATTAGCCGAAGAAGATCTTGATGATCAGCAGCAACACCATGCTTCAGATACTGATCAAGCTAATGGTCATGACACTACTTCTGTTAAAGATGCAGCAGAGGAATCAAAAGCAAATGTGGATGTCAATAATGAAGAAGTTACTCAAGCAGAGAAAAATATTGATGGTCACAATGATCAAGTTGAAGAGGTTAATCACATTAGCAAAGATCATGAAAGCAATGACATTGATCACCACCCAGAAGAGGGTTCGGGTCTGACAAGCGAGGAAACAATAGCTGAGGAGGTGATCAAAGAAAAGAAAGAAGATGAAAACAAGAATGAGGTGGATATGGAAGTCGTTGATGGAGGAAGCAATGAGCTTGATCCAAAGGAAGGGCAAGATGGAAATATCAAAGAAGAAGCAAAGCCTGAGGAACCAGAGGTAACAGAAACGCCGCAAGCGGCTGCTGCTACTACAGGGAATAACAAGAAGGAGGCGGCTCCTGCATACAATGATGTGATTGAGGAGACTGCGAGTAAGCTGATGGCCCAAGAGAAGAGGAGGAACAAGGTCAAAGCTCTGGTTGGGGCTTTTGAGACTGTCATAGATCATGAATCAGGATCCAAATTAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaM_binding

Protein Analysis

433

Amino Acids

46.93

Weight (kDa)

5.53

Isoelectric Point (pI)

63.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaM_binding PF07839 322 - 424 6.1e-13 Plant calmodulin-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 676
AciI CCGC 4 cut(s) 171, 1121, 1127, 1163
AclWI GGATC 4 cut(s) 279, 1043, 1281, 1294
AcsI RAATTY 1 cut(s) 576
AcuI CTGAAG 2 cut(s) 294, 714
AfaI GTAC 2 cut(s) 25, 532
AfiI CCNNNNNNNGG 4 cut(s) 210, 928, 929, 1058
AgsI TTSAA 5 cut(s) 117, 510, 546, 590, 869
AhlI ACTAGT 2 cut(s) 470, 488
AjnI CCWGG 3 cut(s) 31, 209, 334
AluBI AGCT 9 cut(s) 177, 551, 593, 605, 746, 962, 1045, 1207, 1244
AluI AGCT 9 cut(s) 177, 551, 593, 605, 746, 962, 1045, 1207, 1244
Alw21I GWGCWC 1 cut(s) 553
Alw26I GTCTC 4 cut(s) 238, 345, 1187, 1256
AlwI GGATC 4 cut(s) 279, 1043, 1281, 1294
AlwNI CAGNNNCTG 1 cut(s) 737
Aor13HI TCCGGA 1 cut(s) 676
AoxI GGCC 1 cut(s) 1212
ApeKI GCWGC 4 cut(s) 715, 779, 1130, 1133
ApoI RAATTY 1 cut(s) 576
ArsI GACNNNNNNTTYG 2 cut(s) 181, 213
AspS9I GGNCC 3 cut(s) 314, 679, 1213
AsuHPI GGTGA 5 cut(s) 51, 64, 297, 908, 982
AvaII GGWCC 2 cut(s) 314, 679
AxyI CCTNAGG 1 cut(s) 1095
BamHI GGATCC 1 cut(s) 1286
BanII GRGCYC 1 cut(s) 553
Bbv12I GWGCWC 1 cut(s) 553
BbvCI CCTCAGC 1 cut(s) 963
BbvI GCAGC 4 cut(s) 727, 791, 1117, 1120
BccI CCATC 5 cut(s) 310, 842, 1022, 1061, 1204
BciT130I CCWGG 3 cut(s) 33, 211, 336
BclI TGATCA 5 cut(s) 709, 739, 859, 913, 972
BcoDI GTCTC 4 cut(s) 238, 345, 1187, 1256
BcuI ACTAGT 2 cut(s) 470, 488
BfaI CTAG 2 cut(s) 471, 489
BfmI CTRYAG 1 cut(s) 1140
BglII AGATCT 1 cut(s) 700
BisI GCNGC 7 cut(s) 716, 780, 1121, 1128, 1131, 1134, 1164
BlsI GCNGC 7 cut(s) 717, 781, 1122, 1129, 1132, 1135, 1165
BmcAI AGTACT 1 cut(s) 25
Bme1390I CCNGG 3 cut(s) 33, 211, 336
Bme18I GGWCC 2 cut(s) 314, 679
BmgT120I GGNCC 3 cut(s) 314, 679, 1213
BmiI GGNNCC 5 cut(s) 433, 681, 1101, 1167, 1288
BmrFI CCNGG 3 cut(s) 33, 211, 336
BmsI GCATC 2 cut(s) 399, 766
BoxI GACNNNNGTC 1 cut(s) 663
Bpu10I CCTNAGC 1 cut(s) 963
BpuEI CTTGAG 1 cut(s) 813
BsaBI GATNNNNATC 1 cut(s) 738
BsaI GGTCTC 1 cut(s) 345
BsaJI CCNNGG 4 cut(s) 32, 317, 334, 554
BsaWI WCCGGW 1 cut(s) 676
BsaXI ACNNNNNCTCC 2 cut(s) 239, 269
Bsc4I CCNNNNNNNGG 4 cut(s) 210, 928, 929, 1058
Bse1I ACTGG 3 cut(s) 146, 414, 541
Bse21I CCTNAGG 1 cut(s) 1095
Bse3DI GCAATG 2 cut(s) 910, 1045
Bse8I GATNNNNATC 1 cut(s) 738
BseAI TCCGGA 1 cut(s) 676
BseBI CCWGG 3 cut(s) 33, 211, 336
BseDI CCNNGG 4 cut(s) 32, 317, 334, 554
BseGI GGATG 1 cut(s) 811
BseJI GATNNNNATC 1 cut(s) 738
BseLI CCNNNNNNNGG 4 cut(s) 210, 928, 929, 1058
BseMI GCAATG 2 cut(s) 910, 1045
BseMII CTCAG 2 cut(s) 954, 1086
BseNI ACTGG 3 cut(s) 146, 414, 541
BseRI GAGGAG 7 cut(s) 58, 493, 571, 668, 980, 1205, 1240
BseXI GCAGC 4 cut(s) 727, 791, 1117, 1120
BseYI CCCAGC 1 cut(s) 355
BshFI GGCC 1 cut(s) 1214
BsiHKAI GWGCWC 1 cut(s) 553
BsiSI CCGG 1 cut(s) 677
BslI CCNNNNNNNGG 4 cut(s) 210, 928, 929, 1058
BsmAI GTCTC 4 cut(s) 238, 345, 1187, 1256
BsnI GGCC 1 cut(s) 1214
Bso31I GGTCTC 1 cut(s) 345
Bsp1286I GDGCHC 1 cut(s) 553
Bsp13I TCCGGA 1 cut(s) 676
BspACI CCGC 4 cut(s) 171, 1121, 1127, 1163
BspANI GGCC 1 cut(s) 1214
BspCNI CTCAG 2 cut(s) 955, 1087
BspEI TCCGGA 1 cut(s) 676
BspHI TCATGA 4 cut(s) 616, 754, 895, 1276
BspLI GGNNCC 5 cut(s) 433, 681, 1101, 1167, 1288
BspPI GGATC 4 cut(s) 279, 1043, 1281, 1294
BspTNI GGTCTC 1 cut(s) 345
BsrDI GCAATG 2 cut(s) 910, 1045
BsrI ACTGG 3 cut(s) 146, 414, 541
BssECI CCNNGG 4 cut(s) 32, 317, 334, 554
Bst2UI CCWGG 3 cut(s) 33, 211, 336
Bst4CI ACNGT 2 cut(s) 665, 1267
Bst6I CTCTTC 4 cut(s) 334, 864, 921, 1217
BstC8I GCNNGC 3 cut(s) 67, 175, 1125
BstDEI CTNAG 2 cut(s) 963, 1095
BstDSI CCRYGG 1 cut(s) 317
BstF5I GGATG 1 cut(s) 811
BstMAI GTCTC 4 cut(s) 238, 345, 1187, 1256
BstMWI GCNNNNNNNGC 4 cut(s) 183, 452, 1133, 1169
BstNI CCWGG 3 cut(s) 33, 211, 336
BstPAI GACNNNNGTC 1 cut(s) 663
BstSCI CCNGG 3 cut(s) 31, 209, 334
BstSFI CTRYAG 1 cut(s) 1140
BstV1I GCAGC 4 cut(s) 727, 791, 1117, 1120
BstX2I RGATCY 3 cut(s) 284, 700, 1286
BstYI RGATCY 3 cut(s) 284, 700, 1286
Bsu36I CCTNAGG 1 cut(s) 1095
BsuRI GGCC 1 cut(s) 1214
BtgI CCRYGG 1 cut(s) 317
BtsCI GGATG 1 cut(s) 811
BtsIMutI CAGTG 1 cut(s) 153
Cac8I GCNNGC 3 cut(s) 67, 175, 1125
CaiI CAGNNNCTG 1 cut(s) 737
CciI TCATGA 4 cut(s) 616, 754, 895, 1276
Cfr13I GGNCC 3 cut(s) 314, 679, 1213
Csp6I GTAC 2 cut(s) 24, 531
CviAII CATG 6 cut(s) 230, 617, 725, 755, 896, 1277
CviQI GTAC 2 cut(s) 24, 531
DdeI CTNAG 2 cut(s) 963, 1095
Eam1104I CTCTTC 4 cut(s) 334, 864, 921, 1217
EarI CTCTTC 4 cut(s) 334, 864, 921, 1217
Ecl136II GAGCTC 1 cut(s) 551
Eco24I GRGCYC 1 cut(s) 553
Eco31I GGTCTC 1 cut(s) 345
Eco47I GGWCC 2 cut(s) 314, 679
Eco53kI GAGCTC 1 cut(s) 551
Eco57I CTGAAG 2 cut(s) 294, 714
Eco81I CCTNAGG 1 cut(s) 1095
EcoICRI GAGCTC 1 cut(s) 551
EcoRII CCWGG 3 cut(s) 31, 209, 334
EcoT38I GRGCYC 1 cut(s) 553
FaeI CATG 6 cut(s) 233, 620, 728, 758, 899, 1280
FalI AAGNNNNNCTT 2 cut(s) 687, 719
FatI CATG 6 cut(s) 229, 616, 724, 754, 895, 1276
FbaI TGATCA 5 cut(s) 709, 739, 859, 913, 972
Fnu4HI GCNGC 7 cut(s) 716, 780, 1121, 1128, 1131, 1134, 1164
FokI GGATG 1 cut(s) 818
FriOI GRGCYC 1 cut(s) 553
Fsp4HI GCNGC 7 cut(s) 716, 780, 1121, 1128, 1131, 1134, 1164
FspBI CTAG 2 cut(s) 471, 489
GluI GCNGC 7 cut(s) 716, 780, 1121, 1128, 1131, 1134, 1164
GsaI CCCAGC 1 cut(s) 359
HaeIII GGCC 1 cut(s) 1214
HapII CCGG 1 cut(s) 677
Hin1II CATG 6 cut(s) 233, 620, 728, 758, 899, 1280
HindIII AAGCTT 1 cut(s) 603
HinfI GANTC 6 cut(s) 190, 248, 344, 559, 788, 1280
HpaII CCGG 1 cut(s) 677
HphI GGTGA 5 cut(s) 51, 64, 297, 908, 982
Hpy188I TCNGA 4 cut(s) 238, 555, 733, 942
HpyAV CCTTC 6 cut(s) 204, 318, 511, 683, 1052, 1150
HpyCH4III ACNGT 2 cut(s) 665, 1267
HpyCH4V TGCA 3 cut(s) 455, 779, 1172
HpyF10VI GCNNNNNNNGC 4 cut(s) 183, 452, 1133, 1169
HpyF3I CTNAG 2 cut(s) 963, 1095
Hsp92II CATG 6 cut(s) 233, 620, 728, 758, 899, 1280
Kpn2I TCCGGA 1 cut(s) 676
Ksp22I TGATCA 5 cut(s) 709, 739, 859, 913, 972
LmnI GCTCC 3 cut(s) 406, 556, 1171
Lsp1109I GCAGC 4 cut(s) 727, 791, 1117, 1120
LweI GCATC 2 cut(s) 399, 766
MaeI CTAG 2 cut(s) 471, 489
MaeIII GTNAC 4 cut(s) 563, 823, 851, 1108
MflI RGATCY 3 cut(s) 284, 700, 1286
MhlI GDGCHC 1 cut(s) 553
MluCI AATT 5 cut(s) 576, 595, 686, 1292, 1297
MlyI GAGTC 4 cut(s) 184, 242, 353, 568
MmeI TCCRAC 2 cut(s) 276, 604
MroI TCCGGA 1 cut(s) 676
MseI TTAA 7 cut(s) 218, 384, 582, 771, 876, 1295, 1300
MspI CCGG 1 cut(s) 677
MspR9I CCNGG 3 cut(s) 33, 211, 336
MvaI CCWGG 3 cut(s) 33, 211, 336
MwoI GCNNNNNNNGC 4 cut(s) 183, 452, 1133, 1169
NlaIII CATG 6 cut(s) 233, 620, 728, 758, 899, 1280
NlaIV GGNNCC 5 cut(s) 433, 681, 1101, 1167, 1288
NmuCI GTSAC 2 cut(s) 563, 851
PagI TCATGA 4 cut(s) 616, 754, 895, 1276
PfeI GAWTC 2 cut(s) 788, 1280
PflFI GACNNNGTC 1 cut(s) 205
PkrI GCNGC 7 cut(s) 717, 781, 1122, 1129, 1132, 1135, 1165
PleI GAGTC 4 cut(s) 184, 242, 352, 567
PpsI GAGTC 4 cut(s) 184, 242, 352, 567
PshAI GACNNNNGTC 1 cut(s) 663
Psp124BI GAGCTC 1 cut(s) 553
Psp6I CCWGG 3 cut(s) 31, 209, 334
PspFI CCCAGC 1 cut(s) 355
PspGI CCWGG 3 cut(s) 31, 209, 334
PspN4I GGNNCC 5 cut(s) 433, 681, 1101, 1167, 1288
PspPI GGNCC 3 cut(s) 314, 679, 1213
PstNI CAGNNNCTG 1 cut(s) 737
PsuI RGATCY 3 cut(s) 284, 700, 1286
PsyI GACNNNGTC 1 cut(s) 205
RsaI GTAC 2 cut(s) 25, 532
RsaNI GTAC 2 cut(s) 24, 531
SacI GAGCTC 1 cut(s) 553
SaqAI TTAA 7 cut(s) 218, 384, 582, 771, 876, 1295, 1300
SatI GCNGC 7 cut(s) 716, 780, 1121, 1128, 1131, 1134, 1164
Sau96I GGNCC 3 cut(s) 314, 679, 1213
ScaI AGTACT 1 cut(s) 25
SchI GAGTC 4 cut(s) 184, 242, 353, 568
ScrFI CCNGG 3 cut(s) 33, 211, 336
SduI GDGCHC 1 cut(s) 553
SfaNI GCATC 2 cut(s) 399, 766
SfcI CTRYAG 1 cut(s) 1140
SinI GGWCC 2 cut(s) 314, 679
SmlI CTYRAG 1 cut(s) 828
SmoI CTYRAG 1 cut(s) 828
SpeI ACTAGT 2 cut(s) 470, 488
Sse9I AATT 5 cut(s) 576, 595, 686, 1292, 1297
SsiI CCGC 4 cut(s) 171, 1121, 1127, 1163
SspI AATATT 1 cut(s) 844
SspMI CTAG 2 cut(s) 471, 489
SstI GAGCTC 1 cut(s) 553
StyD4I CCNGG 3 cut(s) 31, 209, 334
TaaI ACNGT 2 cut(s) 665, 1267
TaqI TCGA 1 cut(s) 134
TaqII GACCGA 1 cut(s) 354
TasI AATT 5 cut(s) 576, 595, 686, 1292, 1297
TatI WGTACW 1 cut(s) 23
TauI GCSGC 3 cut(s) 1123, 1130, 1166
TfiI GAWTC 2 cut(s) 788, 1280
Tru1I TTAA 7 cut(s) 218, 384, 582, 771, 876, 1295, 1300
Tru9I TTAA 7 cut(s) 218, 384, 582, 771, 876, 1295, 1300
TscAI CASTG 1 cut(s) 153
TseFI GTSAC 2 cut(s) 563, 851
TseI GCWGC 4 cut(s) 715, 779, 1130, 1133
Tsp45I GTSAC 2 cut(s) 563, 851
TspDTI ATGAA 5 cut(s) 633, 831, 912, 1008, 1293
TspGWI ACGGA 1 cut(s) 334
TspRI CASTG 1 cut(s) 153
Tth111I GACNNNGTC 1 cut(s) 205
VpaK11BI GGWCC 2 cut(s) 314, 679
XapI RAATTY 1 cut(s) 576
XspI CTAG 2 cut(s) 471, 489
ZrmI AGTACT 1 cut(s) 25
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.