MD08G1128300.v1.1

domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0005356
Physical Location & Seq
Reverse (-)
12030580 .. 12032004
1425 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1128300.v1.1.491

Sequence Viewer

Length: 1311 bp
ATGGCAACAAGATCTAAAGATGGCAGCCCTGCTCCAGGGAGGGAGAATAAGATTACATCACCATCATCAACCACCAAAAGGCTCACCAAGTCCCAAACGACAATCACTGAAAAATTCTCTTCAACCTCATCAGCAAAACAAGTCCCAAACTATCTCAGGCCGACGTTAAGCGCCTCGAGGCACGAAAACTCGCTGAGGTCATTGTCCGCTGGAGCCAAAAAACATGGCTCTGAAGATCATGCAGCGTCTCAAAAACCTACATTGAATAGAAGAAGATCTTTTGACAAGCCAGCATCACCTTCTAGGCTTCGGAAGGCATTGGTCTCCCCGGGAAGGGAAGCGTCACTGCGATCGTCGTCAATTTCAGCAAAAACCTCATCTAATGCTCCGAGGCCAAGAATAGAGAGGACAAAATCGAGCACTTCTGGGGCTGGAAAGCCGCAGCTTTTGCATGCAAAGAGTGTGAAGAAGAGCAGCGGAAGCAGTTCGGTTAAGAAGGAGAGTTCATCAGGTTCAACAAGAGCTCCGAAGAGTGAGGACATAAAACATACAATTAATCTCGAAGCTAATTTAGATGCTATAGAGTCTTTGGAACATCATGAGGTTGAAGAGGTATGTAAGATCTCGAGTGAGGAGCATGATAAAGTGATTCTTCCGGATCCGAAAGGCGAGGAGATTGAACACGTTGGTGATCACGACGCTGGCGTTTCTGAGGCTGAAGGGAATAGCGGCGAGGATGAGAAGGTGAAGGCTAGTGAGGGTGTAAATTCTAATGCGGAGGAATTGAAGGAAAATGTGGAGGAAAGAAGCGAAGAAAGCGGAAGTGTATTGACCGAAAAAGAGGAGAAGCAGGCAGATAATGACCAACAAGATGGGGAAGAGAAGGAGAAAAAAATTGATGAAAGCGGCGAGGCAAAGGCGGAAGAGGGTTTGAGAAGTGAAGATCAGGAAACAAAAGTTGAGGTGAAAGAAGAAAAGGAGAAGGAGAGTGAAAGTAAAGAAGAAAATACAAGGGAGGAAAGCAAAAATGTTGAGGAAAAGGCATTGGATGAGAAGGAAAGGGGAGAAGTTGTGGACGGCGGAGTTGAAGAAGCAAAGCCGCAAAAGGCTGCTGAGGTGGAGTCAGAGGAAGCGCCGAAGCAGCGGCAAGCGAGCGAGGGATCATCACAAGGAGGAGGGAGGAAGGACACAGTTCCTGCATACAATGATGTGATTGAGGAGACTACAAACAAGTTGATGGAGAAAAGGAAGAACAAGGTGAAGGCATTAGTTGGGGCATTTGAGACTGTCATAGATTACGAATCCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaM_binding

Protein Analysis

437

Amino Acids

47.54

Weight (kDa)

5.44

Isoelectric Point (pI)

67.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaM_binding PF07839 322 - 431 4.9e-14 Plant calmodulin-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 175
AccIII TCCGGA 1 cut(s) 655
AclWI GGATC 3 cut(s) 653, 666, 1168
AcsI RAATTY 2 cut(s) 113, 766
AcuI CTGAAG 2 cut(s) 252, 738
AfiI CCNNNNNNNGG 4 cut(s) 35, 78, 333, 334
AflIII ACRYGT 1 cut(s) 682
AgsI TTSAA 7 cut(s) 123, 265, 516, 608, 680, 787, 1088
AjnI CCWGG 1 cut(s) 34
AleI CACNNNNGTG 1 cut(s) 687
AluBI AGCT 3 cut(s) 445, 524, 566
AluI AGCT 3 cut(s) 445, 524, 566
Alw21I GWGCWC 2 cut(s) 422, 526
Alw26I GTCTC 4 cut(s) 252, 328, 1214, 1277
AlwI GGATC 3 cut(s) 653, 666, 1168
AlwNI CAGNNNCTG 1 cut(s) 1196
Ama87I CYCGRG 3 cut(s) 175, 328, 625
Aor13HI TCCGGA 1 cut(s) 655
AoxI GGCC 2 cut(s) 158, 392
ApeKI GCWGC 6 cut(s) 24, 242, 442, 474, 1109, 1141
ApoI RAATTY 2 cut(s) 113, 766
AseI ATTAAT 1 cut(s) 555
Asp700I GAANNNNTTC 1 cut(s) 484
AspLEI GCGC 2 cut(s) 173, 1135
AsuC2I CCSGG 2 cut(s) 329, 330
AsuHPI GGTGA 7 cut(s) 51, 76, 288, 701, 757, 976, 1270
AvaI CYCGRG 3 cut(s) 175, 328, 625
BamHI GGATCC 1 cut(s) 658
BanII GRGCYC 1 cut(s) 526
Bbv12I GWGCWC 2 cut(s) 422, 526
BbvCI CCTCAGC 2 cut(s) 194, 1113
BbvI GCAGC 6 cut(s) 36, 254, 454, 486, 1096, 1153
BccI CCATC 4 cut(s) 14, 70, 866, 1231
BceAI ACGGC 1 cut(s) 1093
BciT130I CCWGG 1 cut(s) 36
BclI TGATCA 1 cut(s) 691
BcnI CCSGG 2 cut(s) 329, 330
BcoDI GTCTC 4 cut(s) 252, 328, 1214, 1277
BfaI CTAG 2 cut(s) 303, 753
BfmI CTRYAG 1 cut(s) 579
BfoI RGCGCY 2 cut(s) 174, 1136
BglII AGATCT 3 cut(s) 11, 275, 621
Bme1390I CCNGG 3 cut(s) 36, 329, 330
BmeT110I CYCGRG 3 cut(s) 175, 328, 625
BmiI GGNNCC 2 cut(s) 214, 660
BmrFI CCNGG 3 cut(s) 36, 329, 330
BmsI GCATC 2 cut(s) 302, 565
BpmI CTGGAG 2 cut(s) 18, 231
Bpu10I CCTNAGC 2 cut(s) 194, 1113
BpuMI CCSGG 2 cut(s) 329, 330
BsaI GGTCTC 1 cut(s) 328
BsaJI CCNNGG 4 cut(s) 35, 327, 328, 389
BsaWI WCCGGW 1 cut(s) 655
BsaXI ACNNNNNCTCC 4 cut(s) 508, 538, 1056, 1086
Bsc4I CCNNNNNNNGG 4 cut(s) 35, 78, 333, 334
BseAI TCCGGA 1 cut(s) 655
BseBI CCWGG 1 cut(s) 36
BseDI CCNNGG 4 cut(s) 35, 327, 328, 389
BseGI GGATG 2 cut(s) 742, 1054
BseLI CCNNNNNNNGG 4 cut(s) 35, 78, 333, 334
BseMII CTCAG 4 cut(s) 169, 185, 702, 1104
BseRI GAGGAG 5 cut(s) 647, 686, 857, 1188, 1232
BseXI GCAGC 6 cut(s) 36, 254, 454, 486, 1096, 1153
Bsh1285I CGRYCG 1 cut(s) 353
BshFI GGCC 2 cut(s) 160, 394
BsiEI CGRYCG 1 cut(s) 353
BsiHKAI GWGCWC 2 cut(s) 422, 526
BsiHKCI CYCGRG 3 cut(s) 175, 328, 625
BsiSI CCGG 2 cut(s) 329, 656
BslFI GGGAC 2 cut(s) 76, 128
BslI CCNNNNNNNGG 4 cut(s) 35, 78, 333, 334
BsmAI GTCTC 4 cut(s) 252, 328, 1214, 1277
BsmBI CGTCTC 1 cut(s) 252
BsmFI GGGAC 2 cut(s) 76, 128
BsnI GGCC 2 cut(s) 160, 394
Bso31I GGTCTC 1 cut(s) 328
BsoBI CYCGRG 3 cut(s) 175, 328, 625
Bsp1286I GDGCHC 2 cut(s) 422, 526
Bsp13I TCCGGA 1 cut(s) 655
Bsp143I GATC 9 cut(s) 11, 235, 275, 350, 621, 658, 691, 943, 1160
BspANI GGCC 2 cut(s) 160, 394
BspCNI CTCAG 4 cut(s) 168, 186, 703, 1105
BspEI TCCGGA 1 cut(s) 655
BspHI TCATGA 1 cut(s) 598
BspLI GGNNCC 2 cut(s) 214, 660
BspPI GGATC 3 cut(s) 653, 666, 1168
BspQI GCTCTTC 1 cut(s) 464
BspTNI GGTCTC 1 cut(s) 328
BssECI CCNNGG 4 cut(s) 35, 327, 328, 389
BssMI GATC 9 cut(s) 11, 235, 275, 350, 621, 658, 691, 943, 1160
Bst2UI CCWGG 1 cut(s) 36
Bst4CI ACNGT 2 cut(s) 1192, 1288
Bst6I CTCTTC 6 cut(s) 124, 464, 524, 603, 873, 918
BstAPI GCANNNNNTGC 1 cut(s) 448
BstC8I GCNNGC 6 cut(s) 291, 453, 703, 852, 1149, 1153
BstDEI CTNAG 4 cut(s) 155, 194, 711, 1113
BstENI CCTNNNNNAGG 1 cut(s) 33
BstF5I GGATG 2 cut(s) 742, 1054
BstH2I RGCGCY 2 cut(s) 174, 1136
BstHHI GCGC 2 cut(s) 173, 1135
BstKTI GATC 9 cut(s) 14, 238, 278, 353, 624, 661, 694, 946, 1163
BstMAI GTCTC 4 cut(s) 252, 328, 1214, 1277
BstMBI GATC 9 cut(s) 11, 235, 275, 350, 621, 658, 691, 943, 1160
BstMCI CGRYCG 1 cut(s) 353
BstMWI GCNNNNNNNGC 4 cut(s) 448, 480, 816, 1141
BstNI CCWGG 1 cut(s) 36
BstNSI RCATGY 1 cut(s) 455
BstSCI CCNGG 3 cut(s) 34, 327, 328
BstSFI CTRYAG 1 cut(s) 579
BstV1I GCAGC 6 cut(s) 36, 254, 454, 486, 1096, 1153
BstX2I RGATCY 4 cut(s) 11, 275, 621, 658
BstXI CCANNNNNNTGG 1 cut(s) 872
BstYI RGATCY 4 cut(s) 11, 275, 621, 658
BsuRI GGCC 2 cut(s) 160, 394
BtsCI GGATG 2 cut(s) 742, 1054
BtsI GCAGTG 1 cut(s) 344
BtsIMutI CAGTG 2 cut(s) 105, 344
Cac8I GCNNGC 6 cut(s) 291, 453, 703, 852, 1149, 1153
CaiI CAGNNNCTG 1 cut(s) 1196
CciI TCATGA 1 cut(s) 598
CfoI GCGC 2 cut(s) 173, 1135
Cfr9I CCCGGG 1 cut(s) 328
CseI GACGC 3 cut(s) 234, 330, 707
CviAII CATG 5 cut(s) 224, 239, 452, 599, 638
DdeI CTNAG 4 cut(s) 155, 194, 711, 1113
DpnI GATC 9 cut(s) 13, 237, 277, 352, 623, 660, 693, 945, 1162
DpnII GATC 9 cut(s) 11, 235, 275, 350, 621, 658, 691, 943, 1160
Eam1104I CTCTTC 6 cut(s) 124, 464, 524, 603, 873, 918
EarI CTCTTC 6 cut(s) 124, 464, 524, 603, 873, 918
EciI GGCGGA 2 cut(s) 935, 1095
Ecl136II GAGCTC 1 cut(s) 524
Eco24I GRGCYC 1 cut(s) 526
Eco31I GGTCTC 1 cut(s) 328
Eco53kI GAGCTC 1 cut(s) 524
Eco57I CTGAAG 2 cut(s) 252, 738
Eco88I CYCGRG 3 cut(s) 175, 328, 625
EcoICRI GAGCTC 1 cut(s) 524
EcoNI CCTNNNNNAGG 1 cut(s) 33
EcoRII CCWGG 1 cut(s) 34
EcoT38I GRGCYC 1 cut(s) 526
Esp3I CGTCTC 1 cut(s) 252
FaeI CATG 5 cut(s) 227, 242, 455, 602, 641
FalI AAGNNNNNCTT 4 cut(s) 262, 294, 636, 668
FaqI GGGAC 2 cut(s) 76, 128
FatI CATG 5 cut(s) 223, 238, 451, 598, 637
FbaI TGATCA 1 cut(s) 691
FokI GGATG 2 cut(s) 749, 1061
FriOI GRGCYC 1 cut(s) 526
FspBI CTAG 2 cut(s) 303, 753
GlaI GCGC 2 cut(s) 172, 1134
GsuI CTGGAG 2 cut(s) 18, 231
HaeII RGCGCY 2 cut(s) 174, 1136
HaeIII GGCC 2 cut(s) 160, 394
HapII CCGG 2 cut(s) 329, 656
HgaI GACGC 3 cut(s) 234, 330, 707
HhaI GCGC 2 cut(s) 173, 1135
Hin1II CATG 5 cut(s) 227, 242, 455, 602, 641
Hin6I GCGC 2 cut(s) 171, 1133
HinP1I GCGC 2 cut(s) 171, 1133
HinfI GANTC 4 cut(s) 584, 649, 1121, 1301
HpaII CCGG 2 cut(s) 329, 656
HphI GGTGA 7 cut(s) 51, 76, 288, 701, 757, 976, 1270
Hpy166II GTNNAC 1 cut(s) 1075
Hpy188I TCNGA 7 cut(s) 232, 312, 390, 528, 663, 712, 1126
Hpy188III TCNNGA 6 cut(s) 560, 599, 625, 656, 695, 947
Hpy8I GTNNAC 1 cut(s) 1075
Hpy99I CGWCG 3 cut(s) 166, 358, 701
HpyCH4III ACNGT 2 cut(s) 1192, 1288
HpyCH4IV ACGT 2 cut(s) 164, 684
HpyCH4V TGCA 4 cut(s) 242, 451, 455, 1199
HpyF10VI GCNNNNNNNGC 4 cut(s) 448, 480, 816, 1141
HpyF3I CTNAG 4 cut(s) 155, 194, 711, 1113
HpySE526I ACGT 2 cut(s) 164, 684
Hsp92II CATG 5 cut(s) 227, 242, 455, 602, 641
HspAI GCGC 2 cut(s) 171, 1133
Kpn2I TCCGGA 1 cut(s) 655
Ksp22I TGATCA 1 cut(s) 691
Kzo9I GATC 9 cut(s) 11, 235, 275, 350, 621, 658, 691, 943, 1160
LguI GCTCTTC 1 cut(s) 464
LmnI GCTCC 5 cut(s) 37, 212, 391, 529, 634
Lsp1109I GCAGC 6 cut(s) 36, 254, 454, 486, 1096, 1153
LweI GCATC 2 cut(s) 302, 565
MaeI CTAG 2 cut(s) 303, 753
MaeII ACGT 2 cut(s) 164, 684
MaeIII GTNAC 1 cut(s) 342
MalI GATC 9 cut(s) 13, 237, 277, 352, 623, 660, 693, 945, 1162
MboI GATC 9 cut(s) 11, 235, 275, 350, 621, 658, 691, 943, 1160
MflI RGATCY 4 cut(s) 11, 275, 621, 658
MhlI GDGCHC 2 cut(s) 422, 526
MluCI AATT 7 cut(s) 113, 360, 552, 568, 766, 782, 894
MlyI GAGTC 2 cut(s) 593, 1130
MroI TCCGGA 1 cut(s) 655
MroXI GAANNNNTTC 1 cut(s) 484
MseI TTAA 3 cut(s) 167, 492, 555
MslI CAYNNNNRTG 1 cut(s) 687
MspA1I CMGCKG 3 cut(s) 209, 477, 1144
MspI CCGG 2 cut(s) 329, 656
MspR9I CCNGG 3 cut(s) 36, 329, 330
MvaI CCWGG 1 cut(s) 36
MwoI GCNNNNNNNGC 4 cut(s) 448, 480, 816, 1141
NciI CCSGG 2 cut(s) 329, 330
NdeII GATC 9 cut(s) 11, 235, 275, 350, 621, 658, 691, 943, 1160
NlaIII CATG 5 cut(s) 227, 242, 455, 602, 641
NlaIV GGNNCC 2 cut(s) 214, 660
NmuCI GTSAC 1 cut(s) 342
NspI RCATGY 1 cut(s) 455
OliI CACNNNNGTG 1 cut(s) 687
PaeI GCATGC 1 cut(s) 455
PaeR7I CTCGAG 2 cut(s) 175, 625
PagI TCATGA 1 cut(s) 598
PciSI GCTCTTC 1 cut(s) 464
PcsI WCGNNNNNNNCGW 1 cut(s) 702
PdmI GAANNNNTTC 1 cut(s) 484
PfeI GAWTC 2 cut(s) 649, 1301
Ple19I CGATCG 1 cut(s) 353
PleI GAGTC 2 cut(s) 592, 1129
PpsI GAGTC 2 cut(s) 592, 1129
PshBI ATTAAT 1 cut(s) 555
Psp124BI GAGCTC 1 cut(s) 526
Psp6I CCWGG 1 cut(s) 34
PspGI CCWGG 1 cut(s) 34
PspN4I GGNNCC 2 cut(s) 214, 660
PspXI VCTCGAGB 1 cut(s) 175
PstNI CAGNNNCTG 1 cut(s) 1196
PsuI RGATCY 4 cut(s) 11, 275, 621, 658
PvuI CGATCG 1 cut(s) 353
RseI CAYNNNNRTG 1 cut(s) 687
SacI GAGCTC 1 cut(s) 526
SapI GCTCTTC 1 cut(s) 464
SaqAI TTAA 3 cut(s) 167, 492, 555
Sau3AI GATC 9 cut(s) 11, 235, 275, 350, 621, 658, 691, 943, 1160
SchI GAGTC 2 cut(s) 593, 1130
ScrFI CCNGG 3 cut(s) 36, 329, 330
SduI GDGCHC 2 cut(s) 422, 526
SfaNI GCATC 2 cut(s) 302, 565
SfcI CTRYAG 1 cut(s) 579
Sfr274I CTCGAG 2 cut(s) 175, 625
SlaI CTCGAG 2 cut(s) 175, 625
SmaI CCCGGG 1 cut(s) 330
SmiMI CAYNNNNRTG 1 cut(s) 687
SmlI CTYRAG 2 cut(s) 175, 625
SmoI CTYRAG 2 cut(s) 175, 625
SphI GCATGC 1 cut(s) 455
Sse9I AATT 7 cut(s) 113, 360, 552, 568, 766, 782, 894
SspMI CTAG 2 cut(s) 303, 753
SstI GAGCTC 1 cut(s) 526
StyD4I CCNGG 3 cut(s) 34, 327, 328
TaaI ACNGT 2 cut(s) 1192, 1288
TaiI ACGT 2 cut(s) 167, 687
TaqI TCGA 4 cut(s) 176, 416, 561, 626
TaqII GACCGA 1 cut(s) 848
TasI AATT 7 cut(s) 113, 360, 552, 568, 766, 782, 894
TauI GCSGC 5 cut(s) 442, 732, 909, 1102, 1147
TfiI GAWTC 2 cut(s) 649, 1301
Tru1I TTAA 3 cut(s) 167, 492, 555
Tru9I TTAA 3 cut(s) 167, 492, 555
TscAI CASTG 2 cut(s) 112, 351
TseFI GTSAC 1 cut(s) 342
TseI GCWGC 6 cut(s) 24, 242, 442, 474, 1109, 1141
Tsp45I GTSAC 1 cut(s) 342
TspDTI ATGAA 2 cut(s) 495, 915
TspMI CCCGGG 1 cut(s) 328
TspRI CASTG 2 cut(s) 112, 351
VspI ATTAAT 1 cut(s) 555
XagI CCTNNNNNAGG 1 cut(s) 33
XapI RAATTY 2 cut(s) 113, 766
XceI RCATGY 1 cut(s) 455
XhoI CTCGAG 2 cut(s) 175, 625
XmaI CCCGGG 1 cut(s) 328
XmnI GAANNNNTTC 1 cut(s) 484
XspI CTAG 2 cut(s) 303, 753
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.