FvH4_2g37381

1,4-alpha-glucan-branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0013627
Physical Location & Seq
Forward (+)
27262824 .. 27270857
8034 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g37381.t1

Sequence Viewer

Length: 2403 bp
ATGCTGGGCTCTTTGGGTCGTCTTCATCTCACTTATTCTTCTTCTTCTGGGTCTTTCTCGTCTTCCAAGGACTACCCAAGTCCGAGGCTATCTCATCCCAAACTACCCTCTTTTCCGCTGCTTCGACATAGAGCACTGTGCCCTCCAACTAATTTAAGATGCGGGAAGTTGGAGCGTGATTGCAAAGTTAGAGCAGTTTTGACAGACGACAGCTCGATAATGACGGTGAATGAAGGAGAGATGGAAGATATTGGTGTTTTGAAAACAGACTCAAACTTGGAGCCGTACAAAGATCACTTCAAGTGTAGAATAAAAAGATATTTGGAGCAGAAACGTCTCTTTGAGAAATATGAAGGAGGTCTAGAGGAATTCGCTCGAGGTTTTGTGAAATTTGGATTTAATAGAGAAGACGGTGGAGTTGTATACCGTGAATGGGCTCCTCTAGCTCAGGAGGCACAACTTGTTGGAGACTTTAATGGATGGGAGGGCTCCAATCACAAGATGGAGAAGAACCAGTTTGGAGTATGGAGTATCAAGATACATGACTTTGGTGGAAATCCAGCTATTCCTCACAATTCAAAGGTTAAGTTCCGTTCCAAGGATCGAAATGGAGTTTGGGTTGATCGTATTCCTGCTTGGATTAAATATGCCACTGCGGATCCAGCAAAGTTTGCAGCTCCATATGATGGTGTGTACTGGGATCCACCACCAACAGAAAGGTATCAGTTTAAGTATGGTCGTCCTCCAAAACCCAAGGCTCCACGAATATATGAGGCCCATGTTGGAATGAGTAGCTCAGAACCCCGCATCAGTTCATACAGAGAATTTGCTGATAATGTTTTGCCTCGTATTCGAGCAAATCACTATAACACAGTCCAGCTGATGGCTGTGATGGAGCATTCCCATTATGCTTCCTTCGGTTATCATGTGACAAATTTCTTTGCCGTAAGCAGTAGATCTGGAACACCTGAGGACCTTAAATATCTAATTGATAAAGCACATTCCCTAGGTTTGCGCGTATTGATGGATGTCATTCACAGTCATGCAAGTAATAATGTCACTGATGGCCTCATTGACTTTGAAGTTGGTCAATGCACACGAGATTCCTACTTCCATGCATGTGATAGAGGCTACCATAAGTTATGGGATAGCCGACTCTTCAACTATGCAAACTGGGAAGTTCTCCGCTTCTTTTTGTCCAACTTAAGGTGGTGGCTGGAGGAGTATCAGTTTGATGGTTTTCGGTTCGATGGAGTAACTTCCATGCTGTATCATCACCATGGCATAAACGTGGCATTTACAGGAGATTATAATGAGTACTTCAGTGAGGCAACAGATGTTGATGCTGTTGTTTATTTGATGCTTGCCAACTATGTGATCCATAAGATACTGCCATCTGCAACTGTAATTGCCGAAGATGCTTCTGGGATGCCTGGACTTGGTCGGCCTGTCTCTGAAGGAGGTGTTGGTTTTGACTACCGGCTGGCAATGGCCATCCCTGATAAGTGGATTGATTATGTGAAAAACAAGAAAGACGAAGAGTGGTCAGTGACAGAAATTTCATGGAGCTTGACAAATAGGAGATACACCGAGAAATGTGTCTCCTATGCTGAGAGTCATGATCAGTCAATTGTGGGTGACAAAACGATTGCATCCTTTTTGATGGATAAAGAAATGTACTCTGGTATGTCGTGTTTGAAGGATGCTTCTCCTACTGTTGATCGCGGGATTGCCCTCCACAAGATGATACATTTTATAACTATGGCATTAGGAGGCGAGGGCTATCTTAACTTTATGGGTAGTGAGTTTGGAAACCCTGAATGGATTGACTTTCCAAGAGATGGCAATGGGTGGAGTTACGAAAAGTGCAGACGCCAATGGAACCTGGTGGATACAGATCATTTAAGATATAAGTTTATGAACGCATTTGACAGAGCTATGAACTTGCTTGATGAGAAATTTTCGTTCCTTTCATCAACAAAGCAGATAGTGAGCAGCACAAATGAGGAAGACAAGGTTATTGTCTTTGAGCGTGGAGATCTGGTTTTTGTATTCAATTTTCATCCAGTAAACACATACGATAGGTACAAAGTGGGGTGTGACTTACCTGGGAAGTACAGAGTTGCACTAGACAGTGATGCTTTCGAATTTGGTGGACATGGAAGAGTAGGGCATGATGTAGACCACTTCACATTTCCAGAAGGAATACCAGGGGTTCCTGGAACAAATTTCAACCGCCGTCCCAACTCCTTCAGAGTACTCTCACCACCGCGAACATCCGTGGTTTATTACCGAGTGGAGAACTCAACAGCAAAAGTTGTGGTTGGGCAGGAGCACAAAGAAGTTGGATCTATGAAACATGAAAATGTTGGTCCATTAGTGAATTTGTCATCGGATAACTGA

Protein Analysis

801

Amino Acids

91.48

Weight (kDa)

6.47

Isoelectric Point (pI)

34.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBM_48 PF02922 130 - 213 4.1e-19 Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Alpha-amylase PF00128 301 - 353 1.2e-08 Alpha amylase, catalytic domain
Alpha-amylase_C PF02806 665 - 765 3e-19 Alpha amylase, C-terminal all-beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013627)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g37381 FvH4_2g37410
malus_domestica MD08G1002300.v1.1
prunus_persica Prupe.1G354000_v2.0.a1 Prupe.1G354000_v2.0.a1 Prupe.1G354000_v2.0.a1
pyrus_communis pycom08g00150
rosa_chinensis RchiOBHm_Chr6g0304311
rosa_laevigata RLG00000010992
rosa_multiflora Rmu_ssc0000218.1_g000037
rosa_roxburghii Rroxscaffold_7G00163850
rosa_rugosa Rorug06G0329900 Rorug06G0330000
rosa_samantha Rh6AG443300 Rh6BG459100 Rh6CG456100 Rh6DG442800
rosa_wichuraiana Rw6G038470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1311, 1757
AccB7I CCANNNNNTGG 3 cut(s) 686, 883, 1841
AccI GTMKAC 2 cut(s) 423, 2181
AccII CGCG 3 cut(s) 1017, 1725, 2272
AciI CCGC 8 cut(s) 116, 162, 656, 805, 1186, 1725, 2236, 2270
AclWI GGATC 7 cut(s) 609, 653, 666, 695, 708, 1372, 2356
AcoI YGGCCR 1 cut(s) 1491
AcsI RAATTY 9 cut(s) 368, 389, 824, 934, 1557, 1958, 2147, 2227, 2383
AcuI CTGAAG 3 cut(s) 1306, 1476, 2236
AcyI GRCGYC 1 cut(s) 1873
AfaI GTAC 7 cut(s) 287, 695, 1319, 1679, 2087, 2117, 2259
AfiI CCNNNNNNNGG 7 cut(s) 433, 598, 686, 883, 1206, 1439, 1841
AflII CTTAAG 1 cut(s) 1204
AgsI TTSAA 8 cut(s) 262, 301, 579, 1082, 1162, 1699, 2056, 2233
AjnI CCWGG 5 cut(s) 1432, 1884, 2107, 2209, 2218
AjuI GAANNNNNNNTTGG 4 cut(s) 598, 630, 1449, 1481
AluBI AGCT 8 cut(s) 213, 446, 563, 677, 795, 880, 1569, 1937
AluI AGCT 8 cut(s) 213, 446, 563, 677, 795, 880, 1569, 1937
Alw21I GWGCWC 2 cut(s) 136, 2337
Alw26I GTCTC 4 cut(s) 341, 462, 1456, 1606
AlwI GGATC 7 cut(s) 609, 653, 666, 695, 708, 1372, 2356
Ama87I CYCGRG 1 cut(s) 375
AoxI GGCC 4 cut(s) 774, 1066, 1445, 1491
ApeKI GCWGC 3 cut(s) 118, 674, 1995
ApoI RAATTY 9 cut(s) 368, 389, 824, 934, 1557, 1958, 2147, 2227, 2383
AspA2I CCTAGG 1 cut(s) 1006
AspLEI GCGC 1 cut(s) 1017
AspS9I GGNCC 3 cut(s) 775, 973, 2372
AsuHPI GGTGA 4 cut(s) 238, 1268, 1649, 2256
AsuII TTCGAA 1 cut(s) 2145
AvaI CYCGRG 1 cut(s) 375
AvaII GGWCC 2 cut(s) 973, 2372
AvrII CCTAGG 1 cut(s) 1006
AxyI CCTNAGG 1 cut(s) 969
BaeGI GKGCMC 1 cut(s) 143
BalI TGGCCA 1 cut(s) 1493
BamHI GGATCC 2 cut(s) 658, 700
BanII GRGCYC 3 cut(s) 11, 439, 491
BauI CACGAG 1 cut(s) 1098
BbsI GAAGAC 4 cut(s) 14, 54, 414, 2016
Bbv12I GWGCWC 2 cut(s) 136, 2337
BbvI GCAGC 3 cut(s) 105, 686, 2007
BceAI ACGGC 3 cut(s) 268, 929, 2223
BciT130I CCWGG 5 cut(s) 1434, 1886, 2109, 2211, 2220
BciVI GTATCC 1 cut(s) 1885
BclI TGATCA 1 cut(s) 1621
BcoDI GTCTC 4 cut(s) 341, 462, 1456, 1606
BfaI CTAG 4 cut(s) 362, 443, 1007, 2129
BfrI CTTAAG 1 cut(s) 1204
BfuI GTATCC 1 cut(s) 1885
BglII AGATCT 2 cut(s) 956, 2038
BisI GCNGC 3 cut(s) 119, 675, 1996
BlnI CCTAGG 1 cut(s) 1006
BlsI GCNGC 3 cut(s) 120, 676, 1997
BmcAI AGTACT 2 cut(s) 1319, 2259
Bme1390I CCNGG 5 cut(s) 1434, 1886, 2109, 2211, 2220
Bme18I GGWCC 2 cut(s) 973, 2372
BmeT110I CYCGRG 1 cut(s) 375
BmgT120I GGNCC 3 cut(s) 775, 973, 2372
BmiI GGNNCC 8 cut(s) 282, 438, 490, 660, 702, 759, 1883, 2217
BmrFI CCNGG 5 cut(s) 1434, 1886, 2109, 2211, 2220
BmrI ACTGGG 2 cut(s) 706, 1183
BmsI GCATC 9 cut(s) 149, 816, 1333, 1350, 1408, 1419, 1661, 1693, 2128
BmuI ACTGGG 2 cut(s) 706, 1183
BpiI GAAGAC 4 cut(s) 14, 54, 414, 2016
BplI GAGNNNNNCTC 2 cut(s) 76, 108
BpmI CTGGAG 1 cut(s) 1238
Bpu10I CCTNAGC 1 cut(s) 447
Bpu14I TTCGAA 1 cut(s) 2145
BsaBI GATNNNNATC 1 cut(s) 1896
BsaHI GRCGYC 1 cut(s) 1873
BsaJI CCNNGG 9 cut(s) 66, 83, 597, 753, 1006, 1279, 2108, 2210, 2280
BsaXI ACNNNNNCTCC 6 cut(s) 603, 633, 1214, 1244, 2028, 2058
Bsc4I CCNNNNNNNGG 7 cut(s) 433, 598, 686, 883, 1206, 1439, 1841
Bse118I RCCGGY 1 cut(s) 1479
Bse1I ACTGG 4 cut(s) 514, 701, 1178, 2066
Bse21I CCTNAGG 1 cut(s) 969
Bse3DI GCAATG 2 cut(s) 1494, 1852
Bse8I GATNNNNATC 1 cut(s) 1896
BseBI CCWGG 5 cut(s) 1434, 1886, 2109, 2211, 2220
BseDI CCNNGG 9 cut(s) 66, 83, 597, 753, 1006, 1279, 2108, 2210, 2280
BseGI GGATG 9 cut(s) 94, 485, 1033, 1434, 1494, 1652, 1708, 2062, 2276
BseJI GATNNNNATC 1 cut(s) 1896
BseLI CCNNNNNNNGG 7 cut(s) 433, 598, 686, 883, 1206, 1439, 1841
BseMI GCAATG 2 cut(s) 1494, 1852
BseMII CTCAG 4 cut(s) 461, 810, 960, 1602
BseNI ACTGG 4 cut(s) 514, 701, 1178, 2066
BseRI GAGGAG 2 cut(s) 429, 1235
BseSI GKGCMC 1 cut(s) 143
BseXI GCAGC 3 cut(s) 105, 686, 2007
BseYI CCCAGC 1 cut(s) 4
BsgI GTGCAG 1 cut(s) 1888
Bsh1236I CGCG 3 cut(s) 1017, 1725, 2272
BshFI GGCC 4 cut(s) 776, 1068, 1447, 1493
BsiHKAI GWGCWC 2 cut(s) 136, 2337
BsiHKCI CYCGRG 1 cut(s) 375
BsiSI CCGG 1 cut(s) 1480
BslFI GGGAC 1 cut(s) 2226
BslI CCNNNNNNNGG 7 cut(s) 433, 598, 686, 883, 1206, 1439, 1841
BsmAI GTCTC 4 cut(s) 341, 462, 1456, 1606
BsmBI CGTCTC 1 cut(s) 341
BsmFI GGGAC 1 cut(s) 2226
BsmI GAATGC 1 cut(s) 898
BsnI GGCC 4 cut(s) 776, 1068, 1447, 1493
BsoBI CYCGRG 1 cut(s) 375
Bsp119I TTCGAA 1 cut(s) 2145
Bsp1286I GDGCHC 6 cut(s) 11, 136, 143, 439, 491, 2337
Bsp19I CCATGG 1 cut(s) 1279
BspACI CCGC 8 cut(s) 116, 162, 656, 805, 1186, 1725, 2236, 2270
BspANI GGCC 4 cut(s) 776, 1068, 1447, 1493
BspCNI CTCAG 4 cut(s) 460, 809, 961, 1603
BspFNI CGCG 3 cut(s) 1017, 1725, 2272
BspHI TCATGA 1 cut(s) 1618
BspLI GGNNCC 8 cut(s) 282, 438, 490, 660, 702, 759, 1883, 2217
BspPI GGATC 7 cut(s) 609, 653, 666, 695, 708, 1372, 2356
BspT104I TTCGAA 1 cut(s) 2145
BspTI CTTAAG 1 cut(s) 1204
BsrDI GCAATG 2 cut(s) 1494, 1852
BsrFI RCCGGY 1 cut(s) 1479
BsrI ACTGG 4 cut(s) 514, 701, 1178, 2066
BssAI RCCGGY 1 cut(s) 1479
BssECI CCNNGG 9 cut(s) 66, 83, 597, 753, 1006, 1279, 2108, 2210, 2280
BssNAI GTATAC 1 cut(s) 424
BssNI GRCGYC 1 cut(s) 1873
BssSI CACGAG 1 cut(s) 1098
BssT1I CCWWGG 5 cut(s) 66, 597, 753, 1006, 1279
Bst1107I GTATAC 1 cut(s) 424
Bst2BI CACGAG 1 cut(s) 1098
Bst2UI CCWGG 5 cut(s) 1434, 1886, 2109, 2211, 2220
Bst4CI ACNGT 9 cut(s) 138, 226, 413, 428, 874, 1040, 1405, 1717, 2135
Bst6I CTCTTC 3 cut(s) 1163, 1533, 2158
BstACI GRCGYC 1 cut(s) 1873
BstAFI CTTAAG 1 cut(s) 1204
BstAPI GCANNNNNTGC 1 cut(s) 671
BstBI TTCGAA 1 cut(s) 2145
BstC8I GCNNGC 2 cut(s) 1365, 1485
BstDEI CTNAG 4 cut(s) 447, 796, 969, 1611
BstDSI CCRYGG 2 cut(s) 1279, 2280
BstF5I GGATG 9 cut(s) 94, 485, 1033, 1434, 1494, 1652, 1708, 2062, 2276
BstFNI CGCG 3 cut(s) 1017, 1725, 2272
BstHHI GCGC 1 cut(s) 1017
BstMAI GTCTC 4 cut(s) 341, 462, 1456, 1606
BstMWI GCNNNNNNNGC 5 cut(s) 443, 452, 662, 671, 1418
BstNI CCWGG 5 cut(s) 1434, 1886, 2109, 2211, 2220
BstNSI RCATGY 1 cut(s) 1122
BstSCI CCNGG 5 cut(s) 1432, 1884, 2107, 2209, 2218
BstSLI GKGCMC 1 cut(s) 143
BstUI CGCG 3 cut(s) 1017, 1725, 2272
BstV1I GCAGC 3 cut(s) 105, 686, 2007
BstV2I GAAGAC 4 cut(s) 14, 54, 414, 2016
BstX2I RGATCY 5 cut(s) 658, 700, 956, 2038, 2348
BstYI RGATCY 5 cut(s) 658, 700, 956, 2038, 2348
BstZ17I GTATAC 1 cut(s) 424
Bsu36I CCTNAGG 1 cut(s) 969
BsuI GTATCC 1 cut(s) 1885
BsuRI GGCC 4 cut(s) 776, 1068, 1447, 1493
BtgI CCRYGG 2 cut(s) 1279, 2280
BtsCI GGATG 9 cut(s) 94, 485, 1033, 1434, 1494, 1652, 1708, 2062, 2276
BtsI GCAGTG 1 cut(s) 651
BtsIMutI CAGTG 6 cut(s) 134, 651, 1059, 1330, 1554, 2140
Cac8I GCNNGC 2 cut(s) 1365, 1485
CciI TCATGA 1 cut(s) 1618
CfoI GCGC 1 cut(s) 1017
Cfr10I RCCGGY 1 cut(s) 1479
Cfr13I GGNCC 3 cut(s) 775, 973, 2372
CseI GACGC 1 cut(s) 1881
CsiI ACCWGGT 1 cut(s) 1884
Csp6I GTAC 7 cut(s) 286, 694, 1318, 1678, 2086, 2116, 2258
CspCI CAANNNNNGTGG 2 cut(s) 2301, 2336
CviQI GTAC 7 cut(s) 286, 694, 1318, 1678, 2086, 2116, 2258
DdeI CTNAG 4 cut(s) 447, 796, 969, 1611
EaeI YGGCCR 1 cut(s) 1491
Eam1104I CTCTTC 3 cut(s) 1163, 1533, 2158
EarI CTCTTC 3 cut(s) 1163, 1533, 2158
Eco130I CCWWGG 5 cut(s) 66, 597, 753, 1006, 1279
Eco24I GRGCYC 3 cut(s) 11, 439, 491
Eco47I GGWCC 2 cut(s) 973, 2372
Eco57I CTGAAG 3 cut(s) 1306, 1476, 2236
Eco81I CCTNAGG 1 cut(s) 969
Eco88I CYCGRG 1 cut(s) 375
EcoO109I RGGNCCY 1 cut(s) 973
EcoRI GAATTC 1 cut(s) 368
EcoRII CCWGG 5 cut(s) 1432, 1884, 2107, 2209, 2218
EcoT14I CCWWGG 5 cut(s) 66, 597, 753, 1006, 1279
EcoT22I ATGCAT 1 cut(s) 1120
EcoT38I GRGCYC 3 cut(s) 11, 439, 491
ErhI CCWWGG 5 cut(s) 66, 597, 753, 1006, 1279
Esp3I CGTCTC 1 cut(s) 341
FaqI GGGAC 1 cut(s) 2226
FauI CCCGC 3 cut(s) 155, 812, 1718
FauNDI CATATG 1 cut(s) 682
FbaI TGATCA 1 cut(s) 1621
FblI GTMKAC 2 cut(s) 423, 2181
Fnu4HI GCNGC 3 cut(s) 119, 675, 1996
FokI GGATG 9 cut(s) 81, 492, 1040, 1441, 1481, 1639, 1715, 2049, 2263
FriOI GRGCYC 3 cut(s) 11, 439, 491
Fsp4HI GCNGC 3 cut(s) 119, 675, 1996
FspBI CTAG 4 cut(s) 362, 443, 1007, 2129
GlaI GCGC 1 cut(s) 1016
GluI GCNGC 3 cut(s) 119, 675, 1996
GsaI CCCAGC 1 cut(s) 8
GsuI CTGGAG 1 cut(s) 1238
HaeIII GGCC 4 cut(s) 776, 1068, 1447, 1493
HapII CCGG 1 cut(s) 1480
HgaI GACGC 1 cut(s) 1881
HhaI GCGC 1 cut(s) 1017
Hin1I GRCGYC 1 cut(s) 1873
Hin6I GCGC 1 cut(s) 1015
HinP1I GCGC 1 cut(s) 1015
HinfI GANTC 4 cut(s) 269, 1103, 1155, 1615
HpaII CCGG 1 cut(s) 1480
HphI GGTGA 4 cut(s) 238, 1268, 1649, 2256
Hpy166II GTNNAC 5 cut(s) 424, 694, 2071, 2156, 2182
Hpy188I TCNGA 5 cut(s) 84, 799, 1456, 2255, 2395
Hpy188III TCNNGA 6 cut(s) 362, 449, 535, 960, 1619, 2198
Hpy8I GTNNAC 5 cut(s) 424, 694, 2071, 2156, 2182
HpyAV CCTTC 7 cut(s) 227, 347, 925, 1451, 1693, 2195, 2260
HpyCH4III ACNGT 9 cut(s) 138, 226, 413, 428, 874, 1040, 1405, 1717, 2135
HpyCH4IV ACGT 2 cut(s) 334, 1290
HpyF10VI GCNNNNNNNGC 5 cut(s) 443, 452, 662, 671, 1418
HpyF3I CTNAG 4 cut(s) 447, 796, 969, 1611
HpySE526I ACGT 2 cut(s) 334, 1290
Hsp92I GRCGYC 1 cut(s) 1873
HspAI GCGC 1 cut(s) 1015
Ksp22I TGATCA 1 cut(s) 1621
Lsp1109I GCAGC 3 cut(s) 105, 686, 2007
LweI GCATC 9 cut(s) 149, 816, 1333, 1350, 1408, 1419, 1661, 1693, 2128
MabI ACCWGGT 1 cut(s) 1884
MaeI CTAG 4 cut(s) 362, 443, 1007, 2129
MaeII ACGT 2 cut(s) 334, 1290
MaeIII GTNAC 7 cut(s) 928, 1057, 1255, 1549, 1637, 1856, 2099
MfeI CAATTG 1 cut(s) 1629
MflI RGATCY 5 cut(s) 658, 700, 956, 2038, 2348
MhlI GDGCHC 6 cut(s) 11, 136, 143, 439, 491, 2337
MlsI TGGCCA 1 cut(s) 1493
MluNI TGGCCA 1 cut(s) 1493
MlyI GAGTC 3 cut(s) 263, 1149, 1624
MmeI TCCRAC 6 cut(s) 150, 170, 445, 763, 1224, 2326
Mox20I TGGCCA 1 cut(s) 1493
Mph1103I ATGCAT 1 cut(s) 1120
MscI TGGCCA 1 cut(s) 1493
MslI CAYNNNNRTG 5 cut(s) 1041, 1119, 1278, 1289, 2364
Msp20I TGGCCA 1 cut(s) 1493
MspA1I CMGCKG 2 cut(s) 118, 880
MspCI CTTAAG 1 cut(s) 1204
MspI CCGG 1 cut(s) 1480
MspR9I CCNGG 5 cut(s) 1434, 1886, 2109, 2211, 2220
MunI CAATTG 1 cut(s) 1629
Mva1269I GAATGC 1 cut(s) 898
MvaI CCWGG 5 cut(s) 1434, 1886, 2109, 2211, 2220
MvnI CGCG 3 cut(s) 1017, 1725, 2272
MwoI GCNNNNNNNGC 5 cut(s) 443, 452, 662, 671, 1418
NcoI CCATGG 1 cut(s) 1279
NdeI CATATG 1 cut(s) 682
NlaIV GGNNCC 8 cut(s) 282, 438, 490, 660, 702, 759, 1883, 2217
NmuCI GTSAC 5 cut(s) 928, 1057, 1549, 1637, 2099
NsiI ATGCAT 1 cut(s) 1120
NspI RCATGY 1 cut(s) 1122
NspV TTCGAA 1 cut(s) 2145
PaeR7I CTCGAG 1 cut(s) 375
PagI TCATGA 1 cut(s) 1618
PctI GAATGC 1 cut(s) 898
PfeI GAWTC 1 cut(s) 1103
PflFI GACNNNGTC 1 cut(s) 1440
PflMI CCANNNNNTGG 3 cut(s) 686, 883, 1841
PfoI TCCNGGA 1 cut(s) 2218
PkrI GCNGC 3 cut(s) 120, 676, 1997
PleI GAGTC 3 cut(s) 263, 1149, 1623
PpsI GAGTC 3 cut(s) 263, 1149, 1623
PpuMI RGGWCCY 1 cut(s) 973
PsiI TTATAA 2 cut(s) 1311, 1757
Psp5II RGGWCCY 1 cut(s) 973
Psp6I CCWGG 5 cut(s) 1432, 1884, 2107, 2209, 2218
PspFI CCCAGC 1 cut(s) 4
PspGI CCWGG 5 cut(s) 1432, 1884, 2107, 2209, 2218
PspN4I GGNNCC 8 cut(s) 282, 438, 490, 660, 702, 759, 1883, 2217
PspPI GGNCC 3 cut(s) 775, 973, 2372
PspPPI RGGWCCY 1 cut(s) 973
PspXI VCTCGAGB 1 cut(s) 375
PsuI RGATCY 5 cut(s) 658, 700, 956, 2038, 2348
PsyI GACNNNGTC 1 cut(s) 1440
PvuII CAGCTG 1 cut(s) 880
RsaI GTAC 7 cut(s) 287, 695, 1319, 1679, 2087, 2117, 2259
RsaNI GTAC 7 cut(s) 286, 694, 1318, 1678, 2086, 2116, 2258
RseI CAYNNNNRTG 5 cut(s) 1041, 1119, 1278, 1289, 2364
SatI GCNGC 3 cut(s) 119, 675, 1996
Sau96I GGNCC 3 cut(s) 775, 973, 2372
ScaI AGTACT 2 cut(s) 1319, 2259
SchI GAGTC 3 cut(s) 263, 1149, 1624
ScrFI CCNGG 5 cut(s) 1434, 1886, 2109, 2211, 2220
SduI GDGCHC 6 cut(s) 11, 136, 143, 439, 491, 2337
SexAI ACCWGGT 1 cut(s) 1884
SfaNI GCATC 9 cut(s) 149, 816, 1333, 1350, 1408, 1419, 1661, 1693, 2128
Sfr274I CTCGAG 1 cut(s) 375
SfuI TTCGAA 1 cut(s) 2145
SinI GGWCC 2 cut(s) 973, 2372
SlaI CTCGAG 1 cut(s) 375
SmiMI CAYNNNNRTG 5 cut(s) 1041, 1119, 1278, 1289, 2364
SmlI CTYRAG 2 cut(s) 375, 1204
SmoI CTYRAG 2 cut(s) 375, 1204
SsiI CCGC 8 cut(s) 116, 162, 656, 805, 1186, 1725, 2236, 2270
SspMI CTAG 4 cut(s) 362, 443, 1007, 2129
StyD4I CCNGG 5 cut(s) 1432, 1884, 2107, 2209, 2218
StyI CCWWGG 5 cut(s) 66, 597, 753, 1006, 1279
TaaI ACNGT 9 cut(s) 138, 226, 413, 428, 874, 1040, 1405, 1717, 2135
TaiI ACGT 2 cut(s) 337, 1293
TaqI TCGA 7 cut(s) 124, 215, 376, 604, 853, 1248, 2145
TatI WGTACW 5 cut(s) 693, 1317, 1677, 2115, 2257
TfiI GAWTC 1 cut(s) 1103
TscAI CASTG 6 cut(s) 141, 658, 1066, 1330, 1554, 2140
TseFI GTSAC 5 cut(s) 928, 1057, 1549, 1637, 2099
TseI GCWGC 3 cut(s) 118, 674, 1995
Tsp45I GTSAC 5 cut(s) 928, 1057, 1549, 1637, 2099
TspGWI ACGGA 2 cut(s) 581, 2269
TspRI CASTG 6 cut(s) 141, 658, 1066, 1330, 1554, 2140
Tth111I GACNNNGTC 1 cut(s) 1440
Van91I CCANNNNNTGG 3 cut(s) 686, 883, 1841
Vha464I CTTAAG 1 cut(s) 1204
VpaK11BI GGWCC 2 cut(s) 973, 2372
XapI RAATTY 9 cut(s) 368, 389, 824, 934, 1557, 1958, 2147, 2227, 2383
XbaI TCTAGA 1 cut(s) 361
XceI RCATGY 1 cut(s) 1122
XcmI CCANNNNNNNNNTGG 1 cut(s) 499
XhoI CTCGAG 1 cut(s) 375
XmaJI CCTAGG 1 cut(s) 1006
XmiI GTMKAC 2 cut(s) 423, 2181
XspI CTAG 4 cut(s) 362, 443, 1007, 2129
ZrmI AGTACT 2 cut(s) 1319, 2259
Zsp2I ATGCAT 1 cut(s) 1120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.