Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0013627

Physical Location & Seq

Genomic Coordinates Forward (+)

218526 .. 222291

Gene Structure 3766 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1002300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1923 bp

ATGATGTTGGCACCGACTGGTTTGGTTCTTCGTCATCCCACTCACACTCCTCTATCATTTACCTTTACGAGCACCAACGACGAGTACCCATCCAGACTCGGGCTTGGACTCAGGGGCAAAAAGCAGCAAGCAGTGCGGTTGTTGAGGTTCGGAACATCTCAAAACTCATCATCGCTTCTTCGCAGACGTGGTTCCCCCTCAACAACAACAACAACAAGTGGATGTTCCAAGGCAGAGCCTACTTCCAAAAGTAAAGCTGTTTTCATAGACGACAGCCCCATAATAACAGACACCGACCAAAGCATGGAAAACCTTGGAATCTTGAGCATCGATCAATCCTTGCAACCATACAAGGACCACTTCAACTATAGAATCAACAGATACCTGGATCAGAGAAGGCTTATTGAGACATACGAAGGAGGTCTACAGGAATTTGCTCAAGGTTATTTGAAATTTGGATTTAACAGAGAAGAAGGTGGAATTGTGTACCGTGAATGGGCCCCTGCTGCTCAGGAGGCACAACTTATTGGGGACTTTAATGGATGGGATGGCTCCAAGCACAAGATGGACAAGAACCAATTTGGGGTTTGGAGTATCAAGATACCCGATTCTGGTGAAAATTCAGCCATTCCTCACAATTCAAGGGTTAAATTCCGGTTCAAGCATGGAGGTGGAGTTTGGGTTGATCGCATCCCTGCTTGGATTCAATATGCCACCGTGGACCCAGCAAGGTTTGCAGCACCATATGATGGTGTCTACTGGGATCCACCACCATCAGAAAGGTTTCAGTTCAAGTATCCTCGTCCTCCAAAACCCAAGGCTCCACGAATATATGAGGCACATGTTGGAATGAGTAGCTCAGAACCCCGCATCAGTTCATATAGAGAATTTGCTGATGACGTTCTACCGCGTATCCAAGCAAATAACTATAACACAGTCCAGTTGATGGCCGTGATGGAGCATTCCTATTATGCATCATTCGGTTATCATGTGACAAACTTCTTTGCCGTCAGCAGTAGATCTGGAACACCTGAGGACCTTAAATATCTAATTGACAAAGCCCATTCCCTAGGTTTGCGGGTATTGATGGATGTTATTCACAGCCATGCAAGTAACAACATTACTGATGGCCTCAATGGCTTTGAAGTTGGTCAAAGCTCACAAGAGTCCTACTTCCACACAGGAGATAGAGGCTATCATAAGTTATGGGATAGCCGACTTTTCAACTATGCTAATTGGGAAGTTCTCCGCTTCCTTTTATCCAACTTGAGGTGGTGGCTGGAGGAGTTTAAATTTGATGGTTTTCGATTTGATGGAGTAACTTCAATGTTATATCATCACCACGGGATAAACATGGCATTTTCAGGGGATTATCATGAGTATTTTAGTGAGGCAACAGATGTTGATGCTGTTGTTTATTTGATGCTTGCCAACTATCTGATCCATAAAGTTTTGCCAGATGCAACTGTAATTGCCGAAGATGTTTCTGGTATGCCCGGACTTGGTCGGCCTGTCTCTGAAGGTGGCATTGGCTTTGATTACCGGCTGGCAATGGCCATCCCTGACAAATGGATTGACTATGTGAAAAACAAGAGTGACGAAGAGTGGTCAATGAAGGAAATTTCATGGAACTTGACAAATAGGAGGTACACTGAGAAGTGTATCTCCTACGCTGAAAGTCATGACCAGGCAATTGTGGGTGACAAGACAATTGCATTCTTTTTGATGGATAGAGAAATGTATTCTGGCATGTCTTGTTTAGTTGATGCTTCTCCTACCATTGAGCGAGGGGTTGCGCTTCACAAGATGATACATTTCTTGACTATGGCATTAGGAGGCGAGGGGTATCTCAATTTTATGGGAAATGAGGTAAGCACATTGGCCAAAGTTTCTGCCTGCCAAACTATTGGTGTCACTGTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003844 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0006073 GO:0008150 GO:0008152 GO:0009501 GO:0009507 GO:0009536 GO:0009987 GO:0016740 GO:0016757 GO:0016758 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase Alpha-amylase_C CBM_48

Protein Analysis

641

Amino Acids

72.6

Weight (kDa)

6.27

Isoelectric Point (pI)

41.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_48	PF02922	151 - 234	3.9e-21	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Alpha-amylase	PF00128	324 - 392	5.6e-09	Alpha amylase, catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013627)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g37381 FvH4_2g37410
malus_domestica	MD08G1002300.v1.1
prunus_persica	Prupe.1G354000_v2.0.a1 Prupe.1G354000_v2.0.a1 Prupe.1G354000_v2.0.a1
pyrus_communis	pycom08g00150
rosa_chinensis	RchiOBHm_Chr6g0304311
rosa_laevigata	RLG00000010992
rosa_multiflora	Rmu_ssc0000218.1_g000037
rosa_roxburghii	Rroxscaffold_7G00163850
rosa_rugosa	Rorug06G0329900 Rorug06G0330000
rosa_samantha	Rh6AG443300 Rh6BG459100 Rh6CG456100 Rh6DG442800
rosa_wichuraiana	Rw6G038470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	10
AccB7I	CCANNNNNTGG	3 cut(s)	304, 749, 946
AccI	GTMKAC	2 cut(s)	424, 756
AccII	CGCG	1 cut(s)	910
AciI	CCGC	5 cut(s)	136, 868, 908, 1078, 1249
AclWI	GGATC	4 cut(s)	396, 758, 771, 1435
AcoI	YGGCCR	3 cut(s)	948, 1554, 1881
AcsI	RAATTY	7 cut(s)	431, 452, 619, 650, 887, 1292, 1620
AcuI	CTGAAG	1 cut(s)	1539
AfaI	GTAC	3 cut(s)	86, 488, 1649
AfiI	CCNNNNNNNGG	9 cut(s)	99, 304, 496, 583, 611, 749, 946, 1269, 1502
AflIII	ACRYGT	1 cut(s)	841
AgsI	TTSAA	9 cut(s)	364, 451, 642, 661, 707, 793, 1145, 1225, 1326
AjiI	CACGTC	1 cut(s)	188
AjnI	CCWGG	2 cut(s)	384, 1686
AjuI	GAANNNNNNNTTGG	2 cut(s)	1512, 1544
AluBI	AGCT	3 cut(s)	257, 858, 1158
AluI	AGCT	3 cut(s)	257, 858, 1158
Alw21I	GWGCWC	1 cut(s)	74
Alw26I	GTCTC	2 cut(s)	401, 1519
AlwI	GGATC	4 cut(s)	396, 758, 771, 1435
Ama87I	CYCGRG	1 cut(s)	98
AoxI	GGCC	6 cut(s)	498, 948, 1129, 1508, 1554, 1881
ApaI	GGGCCC	1 cut(s)	502
ApeKI	GCWGC	3 cut(s)	124, 506, 737
ApoI	RAATTY	7 cut(s)	431, 452, 619, 650, 887, 1292, 1620
Asp700I	GAANNNNTTC	1 cut(s)	783
AspA2I	CCTAGG	1 cut(s)	1069
AspLEI	GCGC	1 cut(s)	1798
AspS9I	GGNCC	5 cut(s)	355, 498, 499, 721, 1036
AsuC2I	CCSGG	1 cut(s)	1497
AsuHPI	GGTGA	3 cut(s)	626, 1331, 1712
AvaI	CYCGRG	1 cut(s)	98
AvaII	GGWCC	3 cut(s)	355, 721, 1036
AvrII	CCTAGG	1 cut(s)	1069
AxyI	CCTNAGG	1 cut(s)	1032
BaeGI	GKGCMC	1 cut(s)	502
BalI	TGGCCA	2 cut(s)	1556, 1883
BamHI	GGATCC	1 cut(s)	763
BanI	GGYRCC	1 cut(s)	10
BanII	GRGCYC	1 cut(s)	502
BarI	GAAGNNNNNNTAC	2 cut(s)	408, 440
Bbv12I	GWGCWC	1 cut(s)	74
BbvI	GCAGC	3 cut(s)	136, 493, 749
BceAI	ACGGC	2 cut(s)	935, 992
BciT130I	CCWGG	2 cut(s)	386, 1688
BciVI	GTATCC	2 cut(s)	807, 923
BcnI	CCSGG	1 cut(s)	1497
BcoDI	GTCTC	2 cut(s)	401, 1519
BfaI	CTAG	1 cut(s)	1070
BfmI	CTRYAG	2 cut(s)	367, 425
BfuI	GTATCC	2 cut(s)	807, 923
BglI	GCCNNNNNGGC	1 cut(s)	1137
BglII	AGATCT	1 cut(s)	1019
BisI	GCNGC	3 cut(s)	125, 507, 738
BlnI	CCTAGG	1 cut(s)	1069
BlsI	GCNGC	3 cut(s)	126, 508, 739
Bme1390I	CCNGG	3 cut(s)	386, 1497, 1688
Bme18I	GGWCC	3 cut(s)	355, 721, 1036
BmeT110I	CYCGRG	1 cut(s)	98
BmgBI	CACGTC	1 cut(s)	188
BmgT120I	GGNCC	5 cut(s)	355, 498, 499, 721, 1036
BmiI	GGNNCC	8 cut(s)	12, 193, 500, 501, 553, 723, 765, 822
BmrFI	CCNGG	3 cut(s)	386, 1497, 1688
BmrI	ACTGGG	1 cut(s)	769
BmsI	GCATC	8 cut(s)	336, 699, 879, 983, 1396, 1413, 1450, 1756
BmuI	ACTGGG	1 cut(s)	769
BpmI	CTGGAG	1 cut(s)	1301
Bpu10I	CCTNAGC	1 cut(s)	510
BpuEI	CTTGAG	3 cut(s)	343, 423, 1288
BpuMI	CCSGG	1 cut(s)	1497
Bsa29I	ATCGAT	1 cut(s)	330
BsaJI	CCNNGG	6 cut(s)	228, 313, 717, 816, 1069, 1342
BsaWI	WCCGGW	1 cut(s)	654
BsaXI	ACNNNNNCTCC	6 cut(s)	31, 61, 660, 666, 690, 696
Bsc4I	CCNNNNNNNGG	9 cut(s)	99, 304, 496, 583, 611, 749, 946, 1269, 1502
Bse118I	RCCGGY	1 cut(s)	1542
Bse1I	ACTGG	3 cut(s)	22, 764, 940
Bse21I	CCTNAGG	1 cut(s)	1032
Bse3DI	GCAATG	1 cut(s)	1557
BseBI	CCWGG	2 cut(s)	386, 1688
BseCI	ATCGAT	1 cut(s)	330
BseDI	CCNNGG	6 cut(s)	228, 313, 717, 816, 1069, 1342
BseGI	GGATG	8 cut(s)	34, 89, 227, 548, 553, 690, 1096, 1557
BseLI	CCNNNNNNNGG	9 cut(s)	99, 304, 496, 583, 611, 749, 946, 1269, 1502
BseMI	GCAATG	1 cut(s)	1557
BseMII	CTCAG	5 cut(s)	124, 524, 873, 1023, 1644
BseNI	ACTGG	3 cut(s)	22, 764, 940
BseRI	GAGGAG	2 cut(s)	39, 1298
BseSI	GKGCMC	1 cut(s)	502
BseXI	GCAGC	3 cut(s)	136, 493, 749
BseYI	CCCAGC	1 cut(s)	724
Bsh1236I	CGCG	1 cut(s)	910
BshFI	GGCC	6 cut(s)	500, 950, 1131, 1510, 1556, 1883
BshNI	GGYRCC	1 cut(s)	10
BshVI	ATCGAT	1 cut(s)	330
BsiHKAI	GWGCWC	1 cut(s)	74
BsiHKCI	CYCGRG	1 cut(s)	98
BsiSI	CCGG	3 cut(s)	655, 1497, 1543
BslFI	GGGAC	1 cut(s)	545
BslI	CCNNNNNNNGG	9 cut(s)	99, 304, 496, 583, 611, 749, 946, 1269, 1502
BsmAI	GTCTC	2 cut(s)	401, 1519
BsmFI	GGGAC	1 cut(s)	545
BsmI	GAATGC	2 cut(s)	961, 1715
BsnI	GGCC	6 cut(s)	500, 950, 1131, 1510, 1556, 1883
BsoBI	CYCGRG	1 cut(s)	98
Bsp120I	GGGCCC	1 cut(s)	498
Bsp1286I	GDGCHC	2 cut(s)	74, 502
Bsp143I	GATC	6 cut(s)	331, 388, 685, 763, 1019, 1440
BspACI	CCGC	5 cut(s)	136, 868, 908, 1078, 1249
BspANI	GGCC	6 cut(s)	500, 950, 1131, 1510, 1556, 1883
BspCNI	CTCAG	5 cut(s)	123, 523, 872, 1024, 1645
BspDI	ATCGAT	1 cut(s)	330
BspFNI	CGCG	1 cut(s)	910
BspHI	TCATGA	2 cut(s)	1375, 1681
BspLI	GGNNCC	8 cut(s)	12, 193, 500, 501, 553, 723, 765, 822
BspPI	GGATC	4 cut(s)	396, 758, 771, 1435
BspT107I	GGYRCC	1 cut(s)	10
BsrDI	GCAATG	1 cut(s)	1557
BsrFI	RCCGGY	1 cut(s)	1542
BsrI	ACTGG	3 cut(s)	22, 764, 940
BssAI	RCCGGY	1 cut(s)	1542
BssECI	CCNNGG	6 cut(s)	228, 313, 717, 816, 1069, 1342
BssMI	GATC	6 cut(s)	331, 388, 685, 763, 1019, 1440
BssT1I	CCWWGG	4 cut(s)	228, 313, 816, 1069
Bst2UI	CCWGG	2 cut(s)	386, 1688
Bst4CI	ACNGT	5 cut(s)	491, 718, 937, 1468, 1918
Bst6I	CTCTTC	1 cut(s)	1596
BstAPI	GCANNNNNTGC	2 cut(s)	133, 734
BstC8I	GCNNGC	4 cut(s)	129, 1428, 1548, 1897
BstDEI	CTNAG	5 cut(s)	110, 510, 859, 1032, 1653
BstDSI	CCRYGG	2 cut(s)	717, 1342
BstF5I	GGATG	8 cut(s)	34, 89, 227, 548, 553, 690, 1096, 1557
BstFNI	CGCG	1 cut(s)	910
BstHHI	GCGC	1 cut(s)	1798
BstKTI	GATC	6 cut(s)	334, 391, 688, 766, 1022, 1443
BstMAI	GTCTC	2 cut(s)	401, 1519
BstMBI	GATC	6 cut(s)	331, 388, 685, 763, 1019, 1440
BstMWI	GCNNNNNNNGC	5 cut(s)	133, 506, 515, 734, 1137
BstNI	CCWGG	2 cut(s)	386, 1688
BstNSI	RCATGY	2 cut(s)	845, 1753
BstSCI	CCNGG	3 cut(s)	384, 1495, 1686
BstSFI	CTRYAG	2 cut(s)	367, 425
BstSLI	GKGCMC	1 cut(s)	502
BstUI	CGCG	1 cut(s)	910
BstV1I	GCAGC	3 cut(s)	136, 493, 749
BstX2I	RGATCY	2 cut(s)	763, 1019
BstXI	CCANNNNNNTGG	1 cut(s)	1907
BstYI	RGATCY	2 cut(s)	763, 1019
Bsu15I	ATCGAT	1 cut(s)	330
Bsu36I	CCTNAGG	1 cut(s)	1032
BsuI	GTATCC	2 cut(s)	807, 923
BsuRI	GGCC	6 cut(s)	500, 950, 1131, 1510, 1556, 1883
BsuTUI	ATCGAT	1 cut(s)	330
BtgI	CCRYGG	2 cut(s)	717, 1342
BtgZI	GCGATG	1 cut(s)	156
BtrI	CACGTC	1 cut(s)	188
BtsCI	GGATG	8 cut(s)	34, 89, 227, 548, 553, 690, 1096, 1557
BtsI	GCAGTG	1 cut(s)	138
BtsIMutI	CAGTG	3 cut(s)	138, 1650, 1914
Cac8I	GCNNGC	4 cut(s)	129, 1428, 1548, 1897
CciI	TCATGA	2 cut(s)	1375, 1681
CfoI	GCGC	1 cut(s)	1798
Cfr10I	RCCGGY	1 cut(s)	1542
Cfr13I	GGNCC	5 cut(s)	355, 498, 499, 721, 1036
ClaI	ATCGAT	1 cut(s)	330
Csp6I	GTAC	3 cut(s)	85, 487, 1648
CviQI	GTAC	3 cut(s)	85, 487, 1648
DdeI	CTNAG	5 cut(s)	110, 510, 859, 1032, 1653
DpnI	GATC	6 cut(s)	333, 390, 687, 765, 1021, 1442
DpnII	GATC	6 cut(s)	331, 388, 685, 763, 1019, 1440
DraI	TTTAAA	1 cut(s)	1291
EaeI	YGGCCR	3 cut(s)	948, 1554, 1881
Eam1104I	CTCTTC	1 cut(s)	1596
EarI	CTCTTC	1 cut(s)	1596
Eco130I	CCWWGG	4 cut(s)	228, 313, 816, 1069
Eco24I	GRGCYC	1 cut(s)	502
Eco47I	GGWCC	3 cut(s)	355, 721, 1036
Eco57I	CTGAAG	1 cut(s)	1539
Eco81I	CCTNAGG	1 cut(s)	1032
Eco88I	CYCGRG	1 cut(s)	98
EcoO109I	RGGNCCY	2 cut(s)	499, 1036
EcoRII	CCWGG	2 cut(s)	384, 1686
EcoT14I	CCWWGG	4 cut(s)	228, 313, 816, 1069
EcoT22I	ATGCAT	1 cut(s)	976
EcoT38I	GRGCYC	1 cut(s)	502
ErhI	CCWWGG	4 cut(s)	228, 313, 816, 1069
FalI	AAGNNNNNCTT	2 cut(s)	344, 376
FaqI	GGGAC	1 cut(s)	545
FauI	CCCGC	2 cut(s)	875, 1071
FauNDI	CATATG	1 cut(s)	745
FblI	GTMKAC	2 cut(s)	424, 756
Fnu4HI	GCNGC	3 cut(s)	125, 507, 738
FokI	GGATG	8 cut(s)	21, 76, 234, 555, 560, 677, 1103, 1544
FriOI	GRGCYC	1 cut(s)	502
Fsp4HI	GCNGC	3 cut(s)	125, 507, 738
FspBI	CTAG	1 cut(s)	1070
GlaI	GCGC	1 cut(s)	1797
GluI	GCNGC	3 cut(s)	125, 507, 738
GsaI	CCCAGC	1 cut(s)	728
GsuI	CTGGAG	1 cut(s)	1301
HaeIII	GGCC	6 cut(s)	500, 950, 1131, 1510, 1556, 1883
HapII	CCGG	3 cut(s)	655, 1497, 1543
HhaI	GCGC	1 cut(s)	1798
Hin6I	GCGC	1 cut(s)	1796
HinP1I	GCGC	1 cut(s)	1796
HinfI	GANTC	7 cut(s)	96, 108, 318, 372, 608, 703, 1166
HpaII	CCGG	3 cut(s)	655, 1497, 1543
HphI	GGTGA	3 cut(s)	626, 1331, 1712
Hpy166II	GTNNAC	5 cut(s)	425, 487, 721, 757, 1650
Hpy188I	TCNGA	6 cut(s)	152, 393, 778, 862, 1440, 1519
Hpy188III	TCNNGA	8 cut(s)	93, 322, 512, 598, 1023, 1376, 1682, 1819
Hpy8I	GTNNAC	5 cut(s)	425, 487, 721, 757, 1650
Hpy99I	CGWCG	1 cut(s)	83
HpyAV	CCTTC	5 cut(s)	390, 410, 467, 1514, 1609
HpyCH4III	ACNGT	5 cut(s)	491, 718, 937, 1468, 1918
HpyCH4IV	ACGT	2 cut(s)	187, 900
HpyCH4V	TGCA	6 cut(s)	343, 737, 974, 1109, 1463, 1715
HpyF10VI	GCNNNNNNNGC	5 cut(s)	133, 506, 515, 734, 1137
HpyF3I	CTNAG	5 cut(s)	110, 510, 859, 1032, 1653
HpySE526I	ACGT	2 cut(s)	187, 900
HspAI	GCGC	1 cut(s)	1796
Kzo9I	GATC	6 cut(s)	331, 388, 685, 763, 1019, 1440
LmnI	GCTCC	3 cut(s)	557, 826, 958
Lsp1109I	GCAGC	3 cut(s)	136, 493, 749
LweI	GCATC	8 cut(s)	336, 699, 879, 983, 1396, 1413, 1450, 1756
MaeI	CTAG	1 cut(s)	1070
MaeII	ACGT	2 cut(s)	187, 900
MaeIII	GTNAC	6 cut(s)	991, 1112, 1318, 1595, 1700, 1912
MalI	GATC	6 cut(s)	333, 390, 687, 765, 1021, 1442
MboI	GATC	6 cut(s)	331, 388, 685, 763, 1019, 1440
MboII	GAAGA	5 cut(s)	20, 170, 482, 1490, 1613
MfeI	CAATTG	2 cut(s)	1692, 1710
MflI	RGATCY	2 cut(s)	763, 1019
MhlI	GDGCHC	2 cut(s)	74, 502
MlsI	TGGCCA	2 cut(s)	1556, 1883
MluNI	TGGCCA	2 cut(s)	1556, 1883
MlyI	GAGTC	3 cut(s)	90, 102, 1175
MmeI	TCCRAC	2 cut(s)	826, 1287
Mox20I	TGGCCA	2 cut(s)	1556, 1883
Mph1103I	ATGCAT	1 cut(s)	976
MroXI	GAANNNNTTC	1 cut(s)	783
MscI	TGGCCA	2 cut(s)	1556, 1883
MseI	TTAA	5 cut(s)	462, 537, 648, 1041, 1290
MslI	CAYNNNNRTG	2 cut(s)	669, 1104
Msp20I	TGGCCA	2 cut(s)	1556, 1883
MspI	CCGG	3 cut(s)	655, 1497, 1543
MspR9I	CCNGG	3 cut(s)	386, 1497, 1688
MunI	CAATTG	2 cut(s)	1692, 1710
Mva1269I	GAATGC	2 cut(s)	961, 1715
MvaI	CCWGG	2 cut(s)	386, 1688
MvnI	CGCG	1 cut(s)	910
MwoI	GCNNNNNNNGC	5 cut(s)	133, 506, 515, 734, 1137
NciI	CCSGG	1 cut(s)	1497
NdeI	CATATG	1 cut(s)	745
NdeII	GATC	6 cut(s)	331, 388, 685, 763, 1019, 1440
NlaIV	GGNNCC	8 cut(s)	12, 193, 500, 501, 553, 723, 765, 822
NmuCI	GTSAC	4 cut(s)	991, 1595, 1700, 1912
NsiI	ATGCAT	1 cut(s)	976
NspI	RCATGY	2 cut(s)	845, 1753
PagI	TCATGA	2 cut(s)	1375, 1681
PciI	ACATGT	1 cut(s)	841
PctI	GAATGC	2 cut(s)	961, 1715
PdmI	GAANNNNTTC	1 cut(s)	783
PfeI	GAWTC	4 cut(s)	318, 372, 608, 703
PflFI	GACNNNGTC	1 cut(s)	1503
PflMI	CCANNNNNTGG	3 cut(s)	304, 749, 946
PkrI	GCNGC	3 cut(s)	126, 508, 739
PleI	GAGTC	3 cut(s)	90, 102, 1174
PpsI	GAGTC	3 cut(s)	90, 102, 1174
PpuMI	RGGWCCY	1 cut(s)	1036
PscI	ACATGT	1 cut(s)	841
Psp5II	RGGWCCY	1 cut(s)	1036
Psp6I	CCWGG	2 cut(s)	384, 1686
PspFI	CCCAGC	1 cut(s)	724
PspGI	CCWGG	2 cut(s)	384, 1686
PspN4I	GGNNCC	8 cut(s)	12, 193, 500, 501, 553, 723, 765, 822
PspOMI	GGGCCC	1 cut(s)	498
PspPI	GGNCC	5 cut(s)	355, 498, 499, 721, 1036
PspPPI	RGGWCCY	1 cut(s)	1036
PsuI	RGATCY	2 cut(s)	763, 1019
PsyI	GACNNNGTC	1 cut(s)	1503
RsaI	GTAC	3 cut(s)	86, 488, 1649
RsaNI	GTAC	3 cut(s)	85, 487, 1648
RseI	CAYNNNNRTG	2 cut(s)	669, 1104
SaqAI	TTAA	5 cut(s)	462, 537, 648, 1041, 1290
SatI	GCNGC	3 cut(s)	125, 507, 738
Sau3AI	GATC	6 cut(s)	331, 388, 685, 763, 1019, 1440
Sau96I	GGNCC	5 cut(s)	355, 498, 499, 721, 1036
SchI	GAGTC	3 cut(s)	90, 102, 1175
ScrFI	CCNGG	3 cut(s)	386, 1497, 1688
SduI	GDGCHC	2 cut(s)	74, 502
SfaNI	GCATC	8 cut(s)	336, 699, 879, 983, 1396, 1413, 1450, 1756
SfcI	CTRYAG	2 cut(s)	367, 425
SinI	GGWCC	3 cut(s)	355, 721, 1036
SmiMI	CAYNNNNRTG	2 cut(s)	669, 1104
SmlI	CTYRAG	3 cut(s)	322, 438, 1267
SmoI	CTYRAG	3 cut(s)	322, 438, 1267
SsiI	CCGC	5 cut(s)	136, 868, 908, 1078, 1249
SspMI	CTAG	1 cut(s)	1070
StyD4I	CCNGG	3 cut(s)	384, 1495, 1686
StyI	CCWWGG	4 cut(s)	228, 313, 816, 1069
TaaI	ACNGT	5 cut(s)	491, 718, 937, 1468, 1918
TaiI	ACGT	2 cut(s)	190, 903
TaqI	TCGA	2 cut(s)	330, 1306
TfiI	GAWTC	4 cut(s)	318, 372, 608, 703
Tru1I	TTAA	5 cut(s)	462, 537, 648, 1041, 1290
Tru9I	TTAA	5 cut(s)	462, 537, 648, 1041, 1290
TscAI	CASTG	3 cut(s)	138, 1657, 1921
TseFI	GTSAC	4 cut(s)	991, 1595, 1700, 1912
TseI	GCWGC	3 cut(s)	124, 506, 737
Tsp45I	GTSAC	4 cut(s)	991, 1595, 1700, 1912
TspDTI	ATGAA	4 cut(s)	253, 867, 1614, 1628
TspRI	CASTG	3 cut(s)	138, 1657, 1921
Tth111I	GACNNNGTC	1 cut(s)	1503
Van91I	CCANNNNNTGG	3 cut(s)	304, 749, 946
VpaK11BI	GGWCC	3 cut(s)	355, 721, 1036
XapI	RAATTY	7 cut(s)	431, 452, 619, 650, 887, 1292, 1620
XceI	RCATGY	2 cut(s)	845, 1753
XcmI	CCANNNNNNNNNTGG	1 cut(s)	562
XmaJI	CCTAGG	1 cut(s)	1069
XmiI	GTMKAC	2 cut(s)	424, 756
XmnI	GAANNNNTTC	1 cut(s)	783
XspI	CTAG	1 cut(s)	1070
Zsp2I	ATGCAT	1 cut(s)	976

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.