Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0013627

Physical Location & Seq

Genomic Coordinates Forward (+)

6015901 .. 6021749

Gene Structure 5849 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00163850.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2331 bp

ATGTTGGGTACTTTGGGTCTTCATCTCACTTGTTCTTTTGGGTCTTTATCGTGTTCCAAGGACTACCTGAGTCCGAGTCCGAGGGTAACTCAAACTAAAGCAGTCTCTTTTCAGCTTCGACATAGAGTATTGTCCCCTCCAGGAGGTTTAAGTTGTGGGAAGTTGGAGCGTAGTCCCAGAGTTTCAGCAGTTTTGACAGACGACAGCTCGAGAATGACAGTCAGTGATGAAGAGGTGGAAGATATTGGTGTTCTGAGAACAGATTCAAACTTGGAGCCATACAAAGATCACTTCAAGTGTCGAATAAAACGATATTTGGAGCAGAAGAGTCTCTTTGAGAAATATGAAGGAGGTTTAGAGGAATTCGCGCAAGGTTATGTGAAATTTGGATTTAACAGAGAAGATGGTGGAATTGTGTACCGTGAATGGGCCCCTGCAGCTCAGGAGGCACAACTTGTTGGAGACTTTAATGGATGGGATGGCAGCAAATACGAGATGGAGAAGGACCAATTTGGGGTATGGAGTATTAAGATACCCGATTTTGGTGGAAGTCCAGCTATTCCTCACAATTCAAAGGTTAAATTCCGGTTCAAGCACGGAAATGGAGTTTGGGTTGATCGTATTCCTGCTTGGATTAAATATGCCACTGTGGACCCAACAAGATTTGCAGCTCCATATGATGGTGTGTACTGGGATCCACCACCATCAGAAAGGTATCAGTTTAAGTATGGTCGTCCTCCAAAACCCAAGGCTCCACGAATATATGAGGCCCATGTTGGAATGAGTAGCTCAGAACCCCGCATCAGTTCATACAGAGAATTTGCTGATAATGTTTTGCCTCGTATCCAAGCAAATAACTATAACACAGTCCAGCTGATGGCCGTGATGGAGCATTCCTATTATGCATCCTTCGGTTATCATGTGACAAACTTCTTTGCCGTAAGCAGTAGATCTGGAACACCTGAGGACCTTAAATATCTAATTGATAAAGCACATTCCCTAGGTTTGCGTGTATTGATGGATGTTGTTCACAGCCATGCAAGTAATAACGTCACTGATGGCCTCAGTGGCTTTGAAGTTGGTCAAAGCACACAAGATTCCTATTTCCACACAGGAGATAGAGGCTACCATAAGCTATGGGATAGCCGACTTTTCAACTATGCTAATTGGGAAGTTCTCCGCTTCTTTTTATCCAACTTAAGGTGGTGGCTGGAGGAGTATCAATTTGATGGGTTTCGATTTGATGGAGTAACTTCCATGCTGTATCATCACCATGGCATAAATATGGCATTTACAGGGAATTATGATGAGTACTTCAGTGAGGCAACAGATGTTGATGCTGTTGTTTATTTGATGCTTGCCAACTATCTGATCCATAAAATATTGCCATCTGCAACTGTAATTGCTGAAGATGTTTCTGGGATGCCTGGACTTGGTCGGCCTGTCTCTGAAGGAGGTGTTGGTTTTGACTACCGGCTGGCAATGGCCATCCCTGACAAGTGGATTGATTATGTGAAAAACAAGAAAGATGAAGAGTGGTCAATGAAGGAAATTTCATGGAGATTGACGAATAGGAGATACACCGAGAAGTGTGTTTCCTATGCTGAGAGTCATGACCAGGCAATTGTGGGTGACAAGACGATTGCATTCTTTTTGATGGATAAAGAAATGTACTCTGGCATGTCTTGTTTGAATGAAGCATCTCCTACTGTTGAGCGCGGGGTTGCTCTTCACAAGATGATACATTTTATAACTATGGCATTAGGAGGCGAGGGTTACCTTAACTTTATGGGTAATGAGTTTATGAACGCATTTGACAGAGCTATGCACTTGCTTGATGAGAAATTTTCGTTCCTTTCATCAACGAAGCAGATAGTGAGCAGCACAAATGAAGAAGACAAGGTTATTGTCTTTGAGCGTGGAGATCTAGTTTTTGTATTTAATTTTCATCCAGTAAACACATACGATGGGTACAAAGTGGGGTGTGACTTACCTGGGAAGTACAGAGTTGCACTAGATAGTGATGGTTTTGAATTCGGTGGACATGGAAGAGTAGGCCATGATGTAGACCACTTCACATTCCCAGAAGGAATACCAGGGGTTCCTGAAACAAATTTCAACGGCCGTCCCAACTCCGTCAGAGTACTGTCACCACCTCGAACATGCGTGGTTTATTATCGAGTGGATGAAAGTAAGGAGACTGCTGTGAAGGAGGACTCGGCAGCAGAAGTTGTGGTTGGGCAGGAGAACTCCCAATTACTTGGATCCGTGAAAGATGGAAATGTTGGTCCAGGAGTGGAAGAGACCGGCAAAGTTTCATCAGATGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003844 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0006073 GO:0008150 GO:0008152 GO:0009501 GO:0009507 GO:0009536 GO:0009987 GO:0016740 GO:0016757 GO:0016758 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase Alpha-amylase_C CBM_48

Protein Analysis

776

Amino Acids

87.68

Weight (kDa)

5.58

Isoelectric Point (pI)

33.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_48	PF02922	128 - 211	1.1e-20	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Alpha-amylase	PF00128	301 - 353	3.1e-09	Alpha amylase, catalytic domain
Alpha-amylase_C	PF02806	627 - 727	3.9e-20	Alpha amylase, C-terminal all-beta domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013627)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g37381 FvH4_2g37410
malus_domestica	MD08G1002300.v1.1
prunus_persica	Prupe.1G354000_v2.0.a1 Prupe.1G354000_v2.0.a1 Prupe.1G354000_v2.0.a1
pyrus_communis	pycom08g00150
rosa_chinensis	RchiOBHm_Chr6g0304311
rosa_laevigata	RLG00000010992
rosa_multiflora	Rmu_ssc0000218.1_g000037
rosa_roxburghii	Rroxscaffold_7G00163850
rosa_rugosa	Rorug06G0329900 Rorug06G0330000
rosa_samantha	Rh6AG443300 Rh6BG459100 Rh6CG456100 Rh6DG442800
rosa_wichuraiana	Rw6G038470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1751
AccB7I	CCANNNNNTGG	2 cut(s)	680, 877
AccI	GTMKAC	1 cut(s)	2067
AccII	CGCG	2 cut(s)	368, 1719
AciI	CCGC	3 cut(s)	799, 1180, 1719
AclWI	GGATC	5 cut(s)	689, 702, 1366, 2259, 2272
AcoI	YGGCCR	3 cut(s)	879, 1485, 2122
AcsI	RAATTY	8 cut(s)	362, 383, 581, 818, 1551, 1844, 2033, 2113
AcuI	CTGAAG	3 cut(s)	1300, 1428, 1470
AfaI	GTAC	8 cut(s)	10, 419, 689, 1313, 1673, 1973, 2003, 2145
AfiI	CCNNNNNNNGG	8 cut(s)	143, 427, 514, 542, 680, 877, 1200, 1433
AflII	CTTAAG	1 cut(s)	1198
AgsI	TTSAA	9 cut(s)	267, 295, 573, 592, 1076, 1156, 1693, 2033, 2119
AjnI	CCWGG	6 cut(s)	139, 1426, 1617, 1993, 2095, 2290
AjuI	GAANNNNNNNTTGG	6 cut(s)	592, 624, 1443, 1475, 2220, 2252
AluBI	AGCT	9 cut(s)	115, 207, 440, 557, 671, 789, 874, 1135, 1823
AluI	AGCT	9 cut(s)	115, 207, 440, 557, 671, 789, 874, 1135, 1823
Alw26I	GTCTC	6 cut(s)	109, 335, 456, 1450, 2192, 2297
AlwI	GGATC	5 cut(s)	689, 702, 1366, 2259, 2272
Ama87I	CYCGRG	1 cut(s)	208
AoxI	GGCC	8 cut(s)	429, 768, 879, 1060, 1439, 1485, 2056, 2122
ApaI	GGGCCC	1 cut(s)	433
ApeKI	GCWGC	5 cut(s)	437, 483, 668, 1881, 2222
ApoI	RAATTY	8 cut(s)	362, 383, 581, 818, 1551, 1844, 2033, 2113
Asp700I	GAANNNNTTC	1 cut(s)	262
AspA2I	CCTAGG	1 cut(s)	1000
AspLEI	GCGC	2 cut(s)	370, 1719
AspS9I	GGNCC	7 cut(s)	429, 430, 505, 652, 769, 967, 2288
AsuHPI	GGTGA	3 cut(s)	1262, 1643, 2142
AvaI	CYCGRG	1 cut(s)	208
AvaII	GGWCC	4 cut(s)	505, 652, 967, 2288
AvrII	CCTAGG	1 cut(s)	1000
AxyI	CCTNAGG	1 cut(s)	963
BaeGI	GKGCMC	1 cut(s)	433
BalI	TGGCCA	1 cut(s)	1487
BamHI	GGATCC	2 cut(s)	694, 2264
BanII	GRGCYC	1 cut(s)	433
BbsI	GAAGAC	2 cut(s)	11, 1902
BbvI	GCAGC	5 cut(s)	449, 495, 680, 1893, 2234
BceAI	ACGGC	4 cut(s)	866, 923, 2109, 2137
BciT130I	CCWGG	6 cut(s)	141, 1428, 1619, 1995, 2097, 2292
BciVI	GTATCC	1 cut(s)	854
BcoDI	GTCTC	6 cut(s)	109, 335, 456, 1450, 2192, 2297
BfaI	CTAG	3 cut(s)	1001, 1928, 2015
BfmI	CTRYAG	1 cut(s)	435
BfrI	CTTAAG	1 cut(s)	1198
BfuI	GTATCC	1 cut(s)	854
BglI	GCCNNNNNGGC	1 cut(s)	1068
BglII	AGATCT	2 cut(s)	950, 1924
BisI	GCNGC	5 cut(s)	438, 484, 669, 1882, 2223
BlnI	CCTAGG	1 cut(s)	1000
BlsI	GCNGC	5 cut(s)	439, 485, 670, 1883, 2224
BmcAI	AGTACT	2 cut(s)	1313, 2145
Bme1390I	CCNGG	6 cut(s)	141, 1428, 1619, 1995, 2097, 2292
Bme18I	GGWCC	4 cut(s)	505, 652, 967, 2288
BmeT110I	CYCGRG	1 cut(s)	208
BmgT120I	GGNCC	7 cut(s)	429, 430, 505, 652, 769, 967, 2288
BmiI	GGNNCC	8 cut(s)	276, 431, 432, 654, 696, 753, 2103, 2266
BmrFI	CCNGG	6 cut(s)	141, 1428, 1619, 1995, 2097, 2292
BmrI	ACTGGG	1 cut(s)	700
BmsI	GCATC	6 cut(s)	810, 914, 1327, 1344, 1413, 1709
BmuI	ACTGGG	1 cut(s)	700
BpiI	GAAGAC	2 cut(s)	11, 1902
BplI	GAGNNNNNCTC	2 cut(s)	73, 105
BpmI	CTGGAG	2 cut(s)	123, 1232
Bpu10I	CCTNAGC	1 cut(s)	441
BsaI	GGTCTC	1 cut(s)	2297
BsaJI	CCNNGG	7 cut(s)	57, 80, 747, 1000, 1273, 1994, 2096
BsaWI	WCCGGW	1 cut(s)	585
BsaXI	ACNNNNNCTCC	4 cut(s)	597, 627, 1914, 1944
Bsc4I	CCNNNNNNNGG	8 cut(s)	143, 427, 514, 542, 680, 877, 1200, 1433
Bse118I	RCCGGY	2 cut(s)	1473, 2306
Bse1I	ACTGG	2 cut(s)	695, 1952
Bse21I	CCTNAGG	1 cut(s)	963
Bse3DI	GCAATG	1 cut(s)	1488
BseBI	CCWGG	6 cut(s)	141, 1428, 1619, 1995, 2097, 2292
BseDI	CCNNGG	7 cut(s)	57, 80, 747, 1000, 1273, 1994, 2096
BseGI	GGATG	8 cut(s)	479, 484, 905, 1027, 1428, 1488, 1948, 2191
BseLI	CCNNNNNNNGG	8 cut(s)	143, 427, 514, 542, 680, 877, 1200, 1433
BseMI	GCAATG	1 cut(s)	1488
BseMII	CTCAG	7 cut(s)	59, 245, 455, 804, 954, 1078, 1596
BseNI	ACTGG	2 cut(s)	695, 1952
BseRI	GAGGAG	1 cut(s)	1229
BseSI	GKGCMC	1 cut(s)	433
BseX3I	CGGCCG	1 cut(s)	2122
BseXI	GCAGC	5 cut(s)	449, 495, 680, 1893, 2234
Bsh1236I	CGCG	2 cut(s)	368, 1719
Bsh1285I	CGRYCG	1 cut(s)	2125
BshFI	GGCC	8 cut(s)	431, 770, 881, 1062, 1441, 1487, 2058, 2124
BsiEI	CGRYCG	1 cut(s)	2125
BsiHKCI	CYCGRG	1 cut(s)	208
BsiSI	CCGG	3 cut(s)	586, 1474, 2307
BslFI	GGGAC	3 cut(s)	118, 159, 2112
BslI	CCNNNNNNNGG	8 cut(s)	143, 427, 514, 542, 680, 877, 1200, 1433
BsmAI	GTCTC	6 cut(s)	109, 335, 456, 1450, 2192, 2297
BsmFI	GGGAC	3 cut(s)	118, 159, 2112
BsmI	GAATGC	2 cut(s)	892, 1646
BsnI	GGCC	8 cut(s)	431, 770, 881, 1062, 1441, 1487, 2058, 2124
Bso31I	GGTCTC	1 cut(s)	2297
BsoBI	CYCGRG	1 cut(s)	208
Bsp120I	GGGCCC	1 cut(s)	429
Bsp1286I	GDGCHC	1 cut(s)	433
Bsp143I	GATC	7 cut(s)	286, 616, 694, 950, 1371, 1924, 2264
Bsp19I	CCATGG	1 cut(s)	1273
BspACI	CCGC	3 cut(s)	799, 1180, 1719
BspANI	GGCC	8 cut(s)	431, 770, 881, 1062, 1441, 1487, 2058, 2124
BspCNI	CTCAG	7 cut(s)	60, 246, 454, 803, 955, 1077, 1597
BspFNI	CGCG	2 cut(s)	368, 1719
BspHI	TCATGA	1 cut(s)	1612
BspLI	GGNNCC	8 cut(s)	276, 431, 432, 654, 696, 753, 2103, 2266
BspMAI	CTGCAG	1 cut(s)	439
BspPI	GGATC	5 cut(s)	689, 702, 1366, 2259, 2272
BspQI	GCTCTTC	1 cut(s)	1734
BspTI	CTTAAG	1 cut(s)	1198
BspTNI	GGTCTC	1 cut(s)	2297
BsrDI	GCAATG	1 cut(s)	1488
BsrFI	RCCGGY	2 cut(s)	1473, 2306
BsrI	ACTGG	2 cut(s)	695, 1952
BssAI	RCCGGY	2 cut(s)	1473, 2306
BssECI	CCNNGG	7 cut(s)	57, 80, 747, 1000, 1273, 1994, 2096
BssMI	GATC	7 cut(s)	286, 616, 694, 950, 1371, 1924, 2264
BssT1I	CCWWGG	4 cut(s)	57, 747, 1000, 1273
Bst2UI	CCWGG	6 cut(s)	141, 1428, 1619, 1995, 2097, 2292
Bst4CI	ACNGT	7 cut(s)	220, 422, 649, 868, 1399, 1711, 2148
Bst6I	CTCTTC	6 cut(s)	225, 320, 1527, 1734, 2044, 2295
BstAFI	CTTAAG	1 cut(s)	1198
BstC8I	GCNNGC	2 cut(s)	1359, 1479
BstDEI	CTNAG	7 cut(s)	68, 254, 441, 790, 963, 1064, 1605
BstDSI	CCRYGG	1 cut(s)	1273
BstEII	GGTNACC	1 cut(s)	1775
BstENI	CCTNNNNNAGG	1 cut(s)	141
BstF5I	GGATG	8 cut(s)	479, 484, 905, 1027, 1428, 1488, 1948, 2191
BstFNI	CGCG	2 cut(s)	368, 1719
BstHHI	GCGC	2 cut(s)	370, 1719
BstKTI	GATC	7 cut(s)	289, 619, 697, 953, 1374, 1927, 2267
BstMAI	GTCTC	6 cut(s)	109, 335, 456, 1450, 2192, 2297
BstMBI	GATC	7 cut(s)	286, 616, 694, 950, 1371, 1924, 2264
BstMCI	CGRYCG	1 cut(s)	2125
BstMWI	GCNNNNNNNGC	3 cut(s)	437, 446, 1068
BstNI	CCWGG	6 cut(s)	141, 1428, 1619, 1995, 2097, 2292
BstNSI	RCATGY	2 cut(s)	1684, 2166
BstPI	GGTNACC	1 cut(s)	1775
BstSCI	CCNGG	6 cut(s)	139, 1426, 1617, 1993, 2095, 2290
BstSFI	CTRYAG	1 cut(s)	435
BstSLI	GKGCMC	1 cut(s)	433
BstUI	CGCG	2 cut(s)	368, 1719
BstV1I	GCAGC	5 cut(s)	449, 495, 680, 1893, 2234
BstV2I	GAAGAC	2 cut(s)	11, 1902
BstX2I	RGATCY	4 cut(s)	694, 950, 1924, 2264
BstXI	CCANNNNNNTGG	1 cut(s)	2261
BstYI	RGATCY	4 cut(s)	694, 950, 1924, 2264
BstZI	CGGCCG	1 cut(s)	2122
Bsu36I	CCTNAGG	1 cut(s)	963
BsuI	GTATCC	1 cut(s)	854
BsuRI	GGCC	8 cut(s)	431, 770, 881, 1062, 1441, 1487, 2058, 2124
BtgI	CCRYGG	1 cut(s)	1273
BtsCI	GGATG	8 cut(s)	479, 484, 905, 1027, 1428, 1488, 1948, 2191
BtsIMutI	CAGTG	5 cut(s)	229, 645, 1053, 1072, 1324
Cac8I	GCNNGC	2 cut(s)	1359, 1479
CciI	TCATGA	1 cut(s)	1612
CfoI	GCGC	2 cut(s)	370, 1719
Cfr10I	RCCGGY	2 cut(s)	1473, 2306
Cfr13I	GGNCC	7 cut(s)	429, 430, 505, 652, 769, 967, 2288
Csp6I	GTAC	8 cut(s)	9, 418, 688, 1312, 1672, 1972, 2002, 2144
CviQI	GTAC	8 cut(s)	9, 418, 688, 1312, 1672, 1972, 2002, 2144
DdeI	CTNAG	7 cut(s)	68, 254, 441, 790, 963, 1064, 1605
DpnI	GATC	7 cut(s)	288, 618, 696, 952, 1373, 1926, 2266
DpnII	GATC	7 cut(s)	286, 616, 694, 950, 1371, 1924, 2264
EaeI	YGGCCR	3 cut(s)	879, 1485, 2122
EagI	CGGCCG	1 cut(s)	2122
Eam1104I	CTCTTC	6 cut(s)	225, 320, 1527, 1734, 2044, 2295
EarI	CTCTTC	6 cut(s)	225, 320, 1527, 1734, 2044, 2295
EclXI	CGGCCG	1 cut(s)	2122
Eco130I	CCWWGG	4 cut(s)	57, 747, 1000, 1273
Eco24I	GRGCYC	1 cut(s)	433
Eco31I	GGTCTC	1 cut(s)	2297
Eco47I	GGWCC	4 cut(s)	505, 652, 967, 2288
Eco52I	CGGCCG	1 cut(s)	2122
Eco57I	CTGAAG	3 cut(s)	1300, 1428, 1470
Eco81I	CCTNAGG	1 cut(s)	963
Eco88I	CYCGRG	1 cut(s)	208
Eco91I	GGTNACC	1 cut(s)	1775
EcoNI	CCTNNNNNAGG	1 cut(s)	141
EcoO109I	RGGNCCY	2 cut(s)	430, 967
EcoO65I	GGTNACC	1 cut(s)	1775
EcoRI	GAATTC	2 cut(s)	362, 2033
EcoRII	CCWGG	6 cut(s)	139, 1426, 1617, 1993, 2095, 2290
EcoT14I	CCWWGG	4 cut(s)	57, 747, 1000, 1273
EcoT22I	ATGCAT	1 cut(s)	907
EcoT38I	GRGCYC	1 cut(s)	433
ErhI	CCWWGG	4 cut(s)	57, 747, 1000, 1273
FalI	AAGNNNNNCTT	2 cut(s)	317, 349
FaqI	GGGAC	3 cut(s)	118, 159, 2112
FauI	CCCGC	2 cut(s)	806, 1712
FauNDI	CATATG	1 cut(s)	676
FblI	GTMKAC	1 cut(s)	2067
Fnu4HI	GCNGC	5 cut(s)	438, 484, 669, 1882, 2223
FokI	GGATG	8 cut(s)	486, 491, 892, 1034, 1435, 1475, 1935, 2198
FriOI	GRGCYC	1 cut(s)	433
Fsp4HI	GCNGC	5 cut(s)	438, 484, 669, 1882, 2223
FspBI	CTAG	3 cut(s)	1001, 1928, 2015
GlaI	GCGC	2 cut(s)	369, 1718
GluI	GCNGC	5 cut(s)	438, 484, 669, 1882, 2223
GsuI	CTGGAG	2 cut(s)	123, 1232
HaeIII	GGCC	8 cut(s)	431, 770, 881, 1062, 1441, 1487, 2058, 2124
HapII	CCGG	3 cut(s)	586, 1474, 2307
HhaI	GCGC	2 cut(s)	370, 1719
Hin6I	GCGC	2 cut(s)	368, 1717
HinP1I	GCGC	2 cut(s)	368, 1717
HinfI	GANTC	7 cut(s)	70, 76, 263, 328, 1097, 1609, 2216
HpaII	CCGG	3 cut(s)	586, 1474, 2307
HphI	GGTGA	3 cut(s)	1262, 1643, 2142
Hpy166II	GTNNAC	7 cut(s)	418, 652, 688, 1030, 1957, 2042, 2068
Hpy188I	TCNGA	9 cut(s)	75, 81, 255, 709, 793, 1371, 1450, 2141, 2323
Hpy188III	TCNNGA	5 cut(s)	210, 443, 954, 1613, 2105
Hpy8I	GTNNAC	7 cut(s)	418, 652, 688, 1030, 1957, 2042, 2068
HpyAV	CCTTC	7 cut(s)	341, 496, 919, 1445, 1540, 2081, 2203
HpyCH4III	ACNGT	7 cut(s)	220, 422, 649, 868, 1399, 1711, 2148
HpyCH4IV	ACGT	1 cut(s)	1050
HpyCH4V	TGCA	8 cut(s)	437, 668, 905, 1040, 1394, 1646, 1828, 2012
HpyF10VI	GCNNNNNNNGC	3 cut(s)	437, 446, 1068
HpyF3I	CTNAG	7 cut(s)	68, 254, 441, 790, 963, 1064, 1605
HpySE526I	ACGT	1 cut(s)	1050
HspAI	GCGC	2 cut(s)	368, 1717
Kzo9I	GATC	7 cut(s)	286, 616, 694, 950, 1371, 1924, 2264
LguI	GCTCTTC	1 cut(s)	1734
LmnI	GCTCC	6 cut(s)	166, 274, 319, 676, 757, 889
Lsp1109I	GCAGC	5 cut(s)	449, 495, 680, 1893, 2234
LweI	GCATC	6 cut(s)	810, 914, 1327, 1344, 1413, 1709
MaeI	CTAG	3 cut(s)	1001, 1928, 2015
MaeII	ACGT	1 cut(s)	1050
MaeIII	GTNAC	8 cut(s)	85, 922, 1051, 1249, 1631, 1775, 1985, 2148
MalI	GATC	7 cut(s)	288, 618, 696, 952, 1373, 1926, 2266
MboI	GATC	7 cut(s)	286, 616, 694, 950, 1371, 1924, 2264
MfeI	CAATTG	1 cut(s)	1623
MflI	RGATCY	4 cut(s)	694, 950, 1924, 2264
MhlI	GDGCHC	1 cut(s)	433
MlsI	TGGCCA	1 cut(s)	1487
MluNI	TGGCCA	1 cut(s)	1487
MlyI	GAGTC	5 cut(s)	79, 85, 337, 1618, 2210
MmeI	TCCRAC	4 cut(s)	144, 439, 757, 1218
Mox20I	TGGCCA	1 cut(s)	1487
Mph1103I	ATGCAT	1 cut(s)	907
MroXI	GAANNNNTTC	1 cut(s)	262
MscI	TGGCCA	1 cut(s)	1487
MslI	CAYNNNNRTG	4 cut(s)	600, 1035, 1272, 1283
Msp20I	TGGCCA	1 cut(s)	1487
MspA1I	CMGCKG	1 cut(s)	874
MspCI	CTTAAG	1 cut(s)	1198
MspI	CCGG	3 cut(s)	586, 1474, 2307
MspR9I	CCNGG	6 cut(s)	141, 1428, 1619, 1995, 2097, 2292
MunI	CAATTG	1 cut(s)	1623
Mva1269I	GAATGC	2 cut(s)	892, 1646
MvaI	CCWGG	6 cut(s)	141, 1428, 1619, 1995, 2097, 2292
MvnI	CGCG	2 cut(s)	368, 1719
MwoI	GCNNNNNNNGC	3 cut(s)	437, 446, 1068
NcoI	CCATGG	1 cut(s)	1273
NdeI	CATATG	1 cut(s)	676
NdeII	GATC	7 cut(s)	286, 616, 694, 950, 1371, 1924, 2264
NlaIV	GGNNCC	8 cut(s)	276, 431, 432, 654, 696, 753, 2103, 2266
NmeAIII	GCCGAG	1 cut(s)	2198
NmuCI	GTSAC	5 cut(s)	922, 1051, 1631, 1985, 2148
NsiI	ATGCAT	1 cut(s)	907
NspI	RCATGY	2 cut(s)	1684, 2166
PaeR7I	CTCGAG	1 cut(s)	208
PagI	TCATGA	1 cut(s)	1612
PciSI	GCTCTTC	1 cut(s)	1734
PcsI	WCGNNNNNNNCGW	1 cut(s)	307
PctI	GAATGC	2 cut(s)	892, 1646
PdmI	GAANNNNTTC	1 cut(s)	262
PfeI	GAWTC	2 cut(s)	263, 1097
PflFI	GACNNNGTC	1 cut(s)	1434
PflMI	CCANNNNNTGG	2 cut(s)	680, 877
PfoI	TCCNGGA	2 cut(s)	139, 2290
PkrI	GCNGC	5 cut(s)	439, 485, 670, 1883, 2224
PleI	GAGTC	5 cut(s)	78, 84, 336, 1617, 2210
PpsI	GAGTC	5 cut(s)	78, 84, 336, 1617, 2210
PpuMI	RGGWCCY	1 cut(s)	967
PsiI	TTATAA	1 cut(s)	1751
Psp5II	RGGWCCY	1 cut(s)	967
Psp6I	CCWGG	6 cut(s)	139, 1426, 1617, 1993, 2095, 2290
PspEI	GGTNACC	1 cut(s)	1775
PspGI	CCWGG	6 cut(s)	139, 1426, 1617, 1993, 2095, 2290
PspN4I	GGNNCC	8 cut(s)	276, 431, 432, 654, 696, 753, 2103, 2266
PspOMI	GGGCCC	1 cut(s)	429
PspPI	GGNCC	7 cut(s)	429, 430, 505, 652, 769, 967, 2288
PspPPI	RGGWCCY	1 cut(s)	967
PstI	CTGCAG	1 cut(s)	439
PsuI	RGATCY	4 cut(s)	694, 950, 1924, 2264
PsyI	GACNNNGTC	1 cut(s)	1434
PvuII	CAGCTG	1 cut(s)	874
RsaI	GTAC	8 cut(s)	10, 419, 689, 1313, 1673, 1973, 2003, 2145
RsaNI	GTAC	8 cut(s)	9, 418, 688, 1312, 1672, 1972, 2002, 2144
RseI	CAYNNNNRTG	4 cut(s)	600, 1035, 1272, 1283
SapI	GCTCTTC	1 cut(s)	1734
SatI	GCNGC	5 cut(s)	438, 484, 669, 1882, 2223
Sau3AI	GATC	7 cut(s)	286, 616, 694, 950, 1371, 1924, 2264
Sau96I	GGNCC	7 cut(s)	429, 430, 505, 652, 769, 967, 2288
ScaI	AGTACT	2 cut(s)	1313, 2145
SchI	GAGTC	5 cut(s)	79, 85, 337, 1618, 2210
ScrFI	CCNGG	6 cut(s)	141, 1428, 1619, 1995, 2097, 2292
SduI	GDGCHC	1 cut(s)	433
SfaNI	GCATC	6 cut(s)	810, 914, 1327, 1344, 1413, 1709
SfcI	CTRYAG	1 cut(s)	435
Sfr274I	CTCGAG	1 cut(s)	208
SinI	GGWCC	4 cut(s)	505, 652, 967, 2288
SlaI	CTCGAG	1 cut(s)	208
SmiMI	CAYNNNNRTG	4 cut(s)	600, 1035, 1272, 1283
SmlI	CTYRAG	2 cut(s)	208, 1198
SmoI	CTYRAG	2 cut(s)	208, 1198
SsiI	CCGC	3 cut(s)	799, 1180, 1719
SspI	AATATT	1 cut(s)	1383
SspMI	CTAG	3 cut(s)	1001, 1928, 2015
StyD4I	CCNGG	6 cut(s)	139, 1426, 1617, 1993, 2095, 2290
StyI	CCWWGG	4 cut(s)	57, 747, 1000, 1273
TaaI	ACNGT	7 cut(s)	220, 422, 649, 868, 1399, 1711, 2148
TaiI	ACGT	1 cut(s)	1053
TaqI	TCGA	6 cut(s)	118, 209, 301, 1237, 2158, 2179
TatI	WGTACW	5 cut(s)	687, 1311, 1671, 2001, 2143
TfiI	GAWTC	2 cut(s)	263, 1097
TscAI	CASTG	5 cut(s)	229, 652, 1060, 1072, 1324
TseFI	GTSAC	5 cut(s)	922, 1051, 1631, 1985, 2148
TseI	GCWGC	5 cut(s)	437, 483, 668, 1881, 2222
Tsp45I	GTSAC	5 cut(s)	922, 1051, 1631, 1985, 2148
TspGWI	ACGGA	3 cut(s)	612, 2125, 2257
TspRI	CASTG	5 cut(s)	229, 652, 1060, 1072, 1324
Tth111I	GACNNNGTC	1 cut(s)	1434
Van91I	CCANNNNNTGG	2 cut(s)	680, 877
Vha464I	CTTAAG	1 cut(s)	1198
VpaK11BI	GGWCC	4 cut(s)	505, 652, 967, 2288
XagI	CCTNNNNNAGG	1 cut(s)	141
XapI	RAATTY	8 cut(s)	362, 383, 581, 818, 1551, 1844, 2033, 2113
XceI	RCATGY	2 cut(s)	1684, 2166
XhoI	CTCGAG	1 cut(s)	208
XmaJI	CCTAGG	1 cut(s)	1000
XmiI	GTMKAC	1 cut(s)	2067
XmnI	GAANNNNTTC	1 cut(s)	262
XspI	CTAG	3 cut(s)	1001, 1928, 2015
ZrmI	AGTACT	2 cut(s)	1313, 2145
Zsp2I	ATGCAT	1 cut(s)	907

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.