Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0013627

Physical Location & Seq

Genomic Coordinates Reverse (-)

62603559 .. 62608636

Gene Structure 5078 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G038470.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2460 bp

ATGTTGGGTTCTTTGGGTCTTCATCTCACTTGTTCTTTTGAGTCTTTATCGTGTTCCAAGGACTACCTGAGTCCGAGGGTAACTCCAACTAAAGCAGTCTCTTTTCAGCTTCGACGTGGAGTATTGTCCCCTCCAGGAGGTATAAGATGTGGGAAGTTGGAGCGTAGTCCCAGAGTTTCAGCAGTTTTGACAGACGACAGCTCGAGAATGACAGTCAGTGATGACGAGATGGAAGATATTGGTGTTCTGAGAACAGATTCAAACTTGGAGCCTTACAAAGATCACTTCAAGTATCGAATGAAAAGATATTTGGAGCAGAAGAGTCTCTTTGAGAAACATGAAGGAGGTTTAGAGGAATTCGCGCAAGGTTATGTGAAATTTGGATTTAACAGAGAAGATGGTGGAATTGTGTACCGTGAATGGGCCCCTGCAGCTCAGGAGGCACAACTTGTTGGAGACTTTAATGGATGGGATGGCGGCAAATACGAGATGGAGAAGGACCAATTTGGGGTATGGAGTATTAAGATACCCGATTTTGGTGGAAGTCCAGCTATTCCTCACAATTCAAAGGTTAAATTCCGGTTCAAGCATGGAAAGGGAGTTTGGGTTGATCGTATTCCTGCTTGGATTAAATATGCCACTGTGGACCCAACAAGATTTGCAGCTCCATATGATGGTGTGTACTGGGATCCACCACCATCAGAAAGGTATCAGTTTAAGTACGATCGTCCTCCAAAACCCAAGGCTCCACGAATATATGAGGCCCATGTTGGAATGAGTAGCTCAGAACCCCGCATCAGTTCATACAGAGAATTTGCTGATAATGTTTTGCCTCGTATCCAAGCAAATAACTATAACACAGTCCAGCTGATGGCCGTGATGGAGCATTCCTATTATGCATCCTTCGGTTATCATGTGACAAACTTCTTTGCCGTAAGCAGTAGATCTGGAACACCTGAGGACCTTAAATATCTAATTGATAAAGCACATTCCCTAGGTTTGCGTGTATTGATGGATGTTGTTCACAGTCATGCAAGTAACAACGCCACTGATGGCCTCTGTGGCTTTGAAGTTGGTCAATGCACACAAGAGTCCTATTTCTACACAGGAGATAGAGGCTACCATAAGCTATGGGATAGCCGACTTTTCAACTATGCTAATTGGGAAGTTCTCCGCTTCTTTTTATCCAACTTAAGGTGGTGGCTGGAGGAGTATCAATTTGATGGGTTTCGATTTGATGGAGTAACTTCCATGCTGTATCATCACCATGGCATAAATATGGCATTTACAGGGAATTATGATGAGTACTTCAGTGAGGCAACAGATGTTGATGCTGTTGTTTATTTGATGCTTGCCAACTATCTGATCCATAAAATATTGCCATCTGCAACTGTAATTGCTGAAGATGTTTCTGGGATGCCTGGACTTGGTCGGCCTGTCTCTGAAGGAGGTGTTGGTTTTGACTACCGGCTGGCAATGGCCATCCCTGACAAGTGGATTGATTATGTGAAAAACAAGAAAGACGAAGAGTGGTCGATGAAGGAAATTTCATGGAGATTGACGAATAGGAGATACACCGAGAAGTGTGTTTCCTATGCTGAGAGTCATGACCAGGCAATTGTGGGTGACAAGACGATTGCATTCTTTTTGATGGATAAAGAAATGTACTCTGGCATGTCTTGTTTGACTGAAGCTTCTCCTACTGTTGTGCGCGGGGTTGCTCTTCACAAGCTCTCATTGTTGTTTTCCACTATTTCAGATGATACATTTTATAACATGGCATTAGGAGGCGAGGGTTACCTTAACTTTATGGGTAATGAGTTTGGACACCCTGAGTGGATCGACTTTCCAAGAGAAGGCAATGGGTGGAGTTATGAAAAGTGCAGACGCCAATGGAACCTGGTGGATACAGATCATTTAAGATATAAGTTTATGAACGCATTTGACAGAGCTATGCACTTGCTTGATGAGAAATTTTCGTTCCTTTCATCAGCGAAGCAGATAGTGAGCAGCACAAATGAAGAAGACAAGGTTATTGTCTTTGAGCGTGGAGATCTAGTTTTTGTATTTAATTTTCATCCAGTAAACACATACGATGGGTACAAAGTGGGGTGTGACTTACCTGGGAAGTACAGAGTTGCACTAGATAGTGATGCTTTTGAATTCGGTGGACATGGAAGAGTAGGCCATGTTGTAGACCACTTCACATTCCCAGAAGGAATACCAGGGGTTCCTGAAACAAATTTCAACGGCCGTCCCAACTCCTTCAGAGTACTCTCACCACCGCGAACATGCGTGGTTTATTATCGAGTGGATGAAAGTAAGGAGACTGCTGTGAAGGAGGACTCGGCAGCAGAAGTTGTGGTTGAGCCGGAGAACTCCCAATTACTTGGATCCGTGAAAGATGGAAATGTTGGTCCAGGAGTGGAAGAGACCGTCAAAGTTTCATCAGATAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003844 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0006073 GO:0008150 GO:0008152 GO:0009501 GO:0009507 GO:0009536 GO:0009987 GO:0016740 GO:0016757 GO:0016758 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase Alpha-amylase_C CBM_48

Protein Analysis

819

Amino Acids

93.21

Weight (kDa)

5.6

Isoelectric Point (pI)

33.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_48	PF02922	126 - 209	5.2e-20	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Alpha-amylase	PF00128	299 - 348	9.5e-09	Alpha amylase, catalytic domain
Alpha-amylase_C	PF02806	670 - 770	2.3e-20	Alpha amylase, C-terminal all-beta domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013627)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g37381 FvH4_2g37410
malus_domestica	MD08G1002300.v1.1
prunus_persica	Prupe.1G354000_v2.0.a1 Prupe.1G354000_v2.0.a1 Prupe.1G354000_v2.0.a1
pyrus_communis	pycom08g00150
rosa_chinensis	RchiOBHm_Chr6g0304311
rosa_laevigata	RLG00000010992
rosa_multiflora	Rmu_ssc0000218.1_g000037
rosa_roxburghii	Rroxscaffold_7G00163850
rosa_rugosa	Rorug06G0329900 Rorug06G0330000
rosa_samantha	Rh6AG443300 Rh6BG459100 Rh6CG456100 Rh6DG442800
rosa_wichuraiana	Rw6G038470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1773
AccB7I	CCANNNNNTGG	2 cut(s)	674, 871
AccI	GTMKAC	1 cut(s)	2196
AccII	CGCG	3 cut(s)	362, 1713, 2287
AciI	CCGC	5 cut(s)	477, 793, 1174, 1713, 2285
AclWI	GGATC	6 cut(s)	683, 696, 1360, 1847, 2388, 2401
AcoI	YGGCCR	3 cut(s)	873, 1479, 2251
AcsI	RAATTY	8 cut(s)	356, 377, 575, 812, 1545, 1973, 2162, 2242
AcuI	CTGAAG	5 cut(s)	1294, 1422, 1464, 1710, 2251
AcyI	GRCGYC	1 cut(s)	1888
AfaI	GTAC	8 cut(s)	413, 683, 722, 1307, 1667, 2102, 2132, 2274
AfiI	CCNNNNNNNGG	9 cut(s)	137, 421, 508, 536, 674, 871, 1194, 1427, 1856
AflII	CTTAAG	1 cut(s)	1192
AgsI	TTSAA	8 cut(s)	261, 289, 567, 586, 1070, 1150, 2162, 2248
AjiI	CACGTC	1 cut(s)	116
AjnI	CCWGG	7 cut(s)	133, 1420, 1611, 1899, 2122, 2224, 2419
AjuI	GAANNNNNNNTTGG	6 cut(s)	293, 325, 586, 618, 1437, 1469
Alw26I	GTCTC	6 cut(s)	103, 329, 450, 1444, 2321, 2426
AlwI	GGATC	6 cut(s)	683, 696, 1360, 1847, 2388, 2401
Ama87I	CYCGRG	1 cut(s)	202
AoxI	GGCC	8 cut(s)	423, 762, 873, 1054, 1433, 1479, 2185, 2251
ApaI	GGGCCC	1 cut(s)	427
ApeKI	GCWGC	4 cut(s)	431, 662, 2010, 2351
ApoI	RAATTY	8 cut(s)	356, 377, 575, 812, 1545, 1973, 2162, 2242
Asp700I	GAANNNNTTC	1 cut(s)	256
AspA2I	CCTAGG	1 cut(s)	994
AspLEI	GCGC	2 cut(s)	364, 1713
AspS9I	GGNCC	7 cut(s)	423, 424, 499, 646, 763, 961, 2417
AsuHPI	GGTGA	3 cut(s)	1256, 1637, 2271
AvaI	CYCGRG	1 cut(s)	202
AvaII	GGWCC	4 cut(s)	499, 646, 961, 2417
AvrII	CCTAGG	1 cut(s)	994
AxyI	CCTNAGG	1 cut(s)	957
BaeGI	GKGCMC	1 cut(s)	427
BalI	TGGCCA	1 cut(s)	1481
BamHI	GGATCC	2 cut(s)	688, 2393
BanII	GRGCYC	1 cut(s)	427
BbsI	GAAGAC	2 cut(s)	11, 2031
BbvI	GCAGC	4 cut(s)	443, 674, 2022, 2363
BceAI	ACGGC	4 cut(s)	860, 917, 2238, 2266
BciT130I	CCWGG	7 cut(s)	135, 1422, 1613, 1901, 2124, 2226, 2421
BciVI	GTATCC	2 cut(s)	848, 1900
BcoDI	GTCTC	6 cut(s)	103, 329, 450, 1444, 2321, 2426
BfaI	CTAG	3 cut(s)	995, 2057, 2144
BfmI	CTRYAG	1 cut(s)	429
BfrI	CTTAAG	1 cut(s)	1192
BfuI	GTATCC	2 cut(s)	848, 1900
BglI	GCCNNNNNGGC	1 cut(s)	1062
BglII	AGATCT	2 cut(s)	944, 2053
BisI	GCNGC	5 cut(s)	432, 478, 663, 2011, 2352
BlnI	CCTAGG	1 cut(s)	994
BlsI	GCNGC	5 cut(s)	433, 479, 664, 2012, 2353
BmcAI	AGTACT	2 cut(s)	1307, 2274
Bme1390I	CCNGG	7 cut(s)	135, 1422, 1613, 1901, 2124, 2226, 2421
Bme18I	GGWCC	4 cut(s)	499, 646, 961, 2417
BmeT110I	CYCGRG	1 cut(s)	202
BmgBI	CACGTC	1 cut(s)	116
BmgT120I	GGNCC	7 cut(s)	423, 424, 499, 646, 763, 961, 2417
BmiI	GGNNCC	9 cut(s)	270, 425, 426, 648, 690, 747, 1898, 2232, 2395
BmrFI	CCNGG	7 cut(s)	135, 1422, 1613, 1901, 2124, 2226, 2421
BmrI	ACTGGG	1 cut(s)	694
BmsI	GCATC	6 cut(s)	804, 908, 1321, 1338, 1407, 2143
BmuI	ACTGGG	1 cut(s)	694
BpiI	GAAGAC	2 cut(s)	11, 2031
BplI	GAGNNNNNCTC	2 cut(s)	67, 99
BpmI	CTGGAG	2 cut(s)	117, 1226
Bpu10I	CCTNAGC	1 cut(s)	435
BsaBI	GATNNNNATC	1 cut(s)	1911
BsaHI	GRCGYC	1 cut(s)	1888
BsaI	GGTCTC	1 cut(s)	2426
BsaJI	CCNNGG	7 cut(s)	57, 74, 741, 994, 1267, 2123, 2225
BsaWI	WCCGGW	1 cut(s)	579
BsaXI	ACNNNNNCTCC	4 cut(s)	591, 621, 2043, 2073
Bsc4I	CCNNNNNNNGG	9 cut(s)	137, 421, 508, 536, 674, 871, 1194, 1427, 1856
Bse118I	RCCGGY	1 cut(s)	1467
Bse1I	ACTGG	2 cut(s)	689, 2081
Bse21I	CCTNAGG	1 cut(s)	957
Bse3DI	GCAATG	2 cut(s)	1482, 1867
Bse8I	GATNNNNATC	1 cut(s)	1911
BseBI	CCWGG	7 cut(s)	135, 1422, 1613, 1901, 2124, 2226, 2421
BseDI	CCNNGG	7 cut(s)	57, 74, 741, 994, 1267, 2123, 2225
BseGI	GGATG	8 cut(s)	473, 478, 899, 1021, 1422, 1482, 2077, 2320
BseJI	GATNNNNATC	1 cut(s)	1911
BseLI	CCNNNNNNNGG	9 cut(s)	137, 421, 508, 536, 674, 871, 1194, 1427, 1856
BseMI	GCAATG	2 cut(s)	1482, 1867
BseMII	CTCAG	7 cut(s)	59, 239, 449, 798, 948, 1590, 1824
BseNI	ACTGG	2 cut(s)	689, 2081
BseRI	GAGGAG	1 cut(s)	1223
BseSI	GKGCMC	1 cut(s)	427
BseX3I	CGGCCG	1 cut(s)	2251
BseXI	GCAGC	4 cut(s)	443, 674, 2022, 2363
BsgI	GTGCAG	1 cut(s)	1903
Bsh1236I	CGCG	3 cut(s)	362, 1713, 2287
Bsh1285I	CGRYCG	2 cut(s)	727, 2254
BshFI	GGCC	8 cut(s)	425, 764, 875, 1056, 1435, 1481, 2187, 2253
BsiEI	CGRYCG	2 cut(s)	727, 2254
BsiHKCI	CYCGRG	1 cut(s)	202
BsiSI	CCGG	3 cut(s)	580, 1468, 2372
BslFI	GGGAC	3 cut(s)	112, 153, 2241
BslI	CCNNNNNNNGG	9 cut(s)	137, 421, 508, 536, 674, 871, 1194, 1427, 1856
BsmAI	GTCTC	6 cut(s)	103, 329, 450, 1444, 2321, 2426
BsmFI	GGGAC	3 cut(s)	112, 153, 2241
BsmI	GAATGC	2 cut(s)	886, 1640
BsnI	GGCC	8 cut(s)	425, 764, 875, 1056, 1435, 1481, 2187, 2253
Bso31I	GGTCTC	1 cut(s)	2426
BsoBI	CYCGRG	1 cut(s)	202
Bsp120I	GGGCCC	1 cut(s)	423
Bsp1286I	GDGCHC	1 cut(s)	427
Bsp19I	CCATGG	1 cut(s)	1267
BspACI	CCGC	5 cut(s)	477, 793, 1174, 1713, 2285
BspANI	GGCC	8 cut(s)	425, 764, 875, 1056, 1435, 1481, 2187, 2253
BspCNI	CTCAG	7 cut(s)	60, 240, 448, 797, 949, 1591, 1825
BspFNI	CGCG	3 cut(s)	362, 1713, 2287
BspHI	TCATGA	1 cut(s)	1606
BspLI	GGNNCC	9 cut(s)	270, 425, 426, 648, 690, 747, 1898, 2232, 2395
BspMAI	CTGCAG	1 cut(s)	433
BspPI	GGATC	6 cut(s)	683, 696, 1360, 1847, 2388, 2401
BspQI	GCTCTTC	1 cut(s)	1728
BspTI	CTTAAG	1 cut(s)	1192
BspTNI	GGTCTC	1 cut(s)	2426
BsrDI	GCAATG	2 cut(s)	1482, 1867
BsrFI	RCCGGY	1 cut(s)	1467
BsrI	ACTGG	2 cut(s)	689, 2081
BssAI	RCCGGY	1 cut(s)	1467
BssECI	CCNNGG	7 cut(s)	57, 74, 741, 994, 1267, 2123, 2225
BssNI	GRCGYC	1 cut(s)	1888
BssT1I	CCWWGG	4 cut(s)	57, 741, 994, 1267
Bst2UI	CCWGG	7 cut(s)	135, 1422, 1613, 1901, 2124, 2226, 2421
Bst4CI	ACNGT	8 cut(s)	214, 416, 643, 862, 1028, 1393, 1705, 2437
Bst6I	CTCTTC	5 cut(s)	314, 1521, 1728, 2173, 2424
BstACI	GRCGYC	1 cut(s)	1888
BstAFI	CTTAAG	1 cut(s)	1192
BstC8I	GCNNGC	2 cut(s)	1353, 1473
BstDEI	CTNAG	7 cut(s)	68, 248, 435, 784, 957, 1599, 1833
BstDSI	CCRYGG	1 cut(s)	1267
BstEII	GGTNACC	1 cut(s)	1796
BstENI	CCTNNNNNAGG	1 cut(s)	135
BstF5I	GGATG	8 cut(s)	473, 478, 899, 1021, 1422, 1482, 2077, 2320
BstFNI	CGCG	3 cut(s)	362, 1713, 2287
BstHHI	GCGC	2 cut(s)	364, 1713
BstMAI	GTCTC	6 cut(s)	103, 329, 450, 1444, 2321, 2426
BstMCI	CGRYCG	2 cut(s)	727, 2254
BstMWI	GCNNNNNNNGC	3 cut(s)	431, 440, 1062
BstNI	CCWGG	7 cut(s)	135, 1422, 1613, 1901, 2124, 2226, 2421
BstNSI	RCATGY	2 cut(s)	1678, 2295
BstPI	GGTNACC	1 cut(s)	1796
BstSCI	CCNGG	7 cut(s)	133, 1420, 1611, 1899, 2122, 2224, 2419
BstSFI	CTRYAG	1 cut(s)	429
BstSLI	GKGCMC	1 cut(s)	427
BstUI	CGCG	3 cut(s)	362, 1713, 2287
BstV1I	GCAGC	4 cut(s)	443, 674, 2022, 2363
BstV2I	GAAGAC	2 cut(s)	11, 2031
BstX2I	RGATCY	4 cut(s)	688, 944, 2053, 2393
BstXI	CCANNNNNNTGG	1 cut(s)	2390
BstYI	RGATCY	4 cut(s)	688, 944, 2053, 2393
BstZI	CGGCCG	1 cut(s)	2251
Bsu36I	CCTNAGG	1 cut(s)	957
BsuI	GTATCC	2 cut(s)	848, 1900
BsuRI	GGCC	8 cut(s)	425, 764, 875, 1056, 1435, 1481, 2187, 2253
BtgI	CCRYGG	1 cut(s)	1267
BtrI	CACGTC	1 cut(s)	116
BtsCI	GGATG	8 cut(s)	473, 478, 899, 1021, 1422, 1482, 2077, 2320
BtsIMutI	CAGTG	4 cut(s)	223, 639, 1047, 1318
Cac8I	GCNNGC	2 cut(s)	1353, 1473
CciI	TCATGA	1 cut(s)	1606
CfoI	GCGC	2 cut(s)	364, 1713
Cfr10I	RCCGGY	1 cut(s)	1467
Cfr13I	GGNCC	7 cut(s)	423, 424, 499, 646, 763, 961, 2417
CseI	GACGC	1 cut(s)	1896
CsiI	ACCWGGT	1 cut(s)	1899
Csp6I	GTAC	8 cut(s)	412, 682, 721, 1306, 1666, 2101, 2131, 2273
CviQI	GTAC	8 cut(s)	412, 682, 721, 1306, 1666, 2101, 2131, 2273
DdeI	CTNAG	7 cut(s)	68, 248, 435, 784, 957, 1599, 1833
EaeI	YGGCCR	3 cut(s)	873, 1479, 2251
EagI	CGGCCG	1 cut(s)	2251
Eam1104I	CTCTTC	5 cut(s)	314, 1521, 1728, 2173, 2424
EarI	CTCTTC	5 cut(s)	314, 1521, 1728, 2173, 2424
EclXI	CGGCCG	1 cut(s)	2251
Eco130I	CCWWGG	4 cut(s)	57, 741, 994, 1267
Eco24I	GRGCYC	1 cut(s)	427
Eco31I	GGTCTC	1 cut(s)	2426
Eco47I	GGWCC	4 cut(s)	499, 646, 961, 2417
Eco52I	CGGCCG	1 cut(s)	2251
Eco57I	CTGAAG	5 cut(s)	1294, 1422, 1464, 1710, 2251
Eco81I	CCTNAGG	1 cut(s)	957
Eco88I	CYCGRG	1 cut(s)	202
Eco91I	GGTNACC	1 cut(s)	1796
EcoNI	CCTNNNNNAGG	1 cut(s)	135
EcoO109I	RGGNCCY	2 cut(s)	424, 961
EcoO65I	GGTNACC	1 cut(s)	1796
EcoRI	GAATTC	2 cut(s)	356, 2162
EcoRII	CCWGG	7 cut(s)	133, 1420, 1611, 1899, 2122, 2224, 2419
EcoT14I	CCWWGG	4 cut(s)	57, 741, 994, 1267
EcoT22I	ATGCAT	1 cut(s)	901
EcoT38I	GRGCYC	1 cut(s)	427
ErhI	CCWWGG	4 cut(s)	57, 741, 994, 1267
FalI	AAGNNNNNCTT	2 cut(s)	311, 343
FaqI	GGGAC	3 cut(s)	112, 153, 2241
FauI	CCCGC	2 cut(s)	800, 1706
FauNDI	CATATG	1 cut(s)	670
FblI	GTMKAC	1 cut(s)	2196
Fnu4HI	GCNGC	5 cut(s)	432, 478, 663, 2011, 2352
FokI	GGATG	8 cut(s)	480, 485, 886, 1028, 1429, 1469, 2064, 2327
FriOI	GRGCYC	1 cut(s)	427
Fsp4HI	GCNGC	5 cut(s)	432, 478, 663, 2011, 2352
FspBI	CTAG	3 cut(s)	995, 2057, 2144
GlaI	GCGC	2 cut(s)	363, 1712
GluI	GCNGC	5 cut(s)	432, 478, 663, 2011, 2352
GsuI	CTGGAG	2 cut(s)	117, 1226
HaeIII	GGCC	8 cut(s)	425, 764, 875, 1056, 1435, 1481, 2187, 2253
HapII	CCGG	3 cut(s)	580, 1468, 2372
HgaI	GACGC	1 cut(s)	1896
HhaI	GCGC	2 cut(s)	364, 1713
Hin1I	GRCGYC	1 cut(s)	1888
Hin6I	GCGC	2 cut(s)	362, 1711
HinP1I	GCGC	2 cut(s)	362, 1711
HindIII	AAGCTT	1 cut(s)	1692
HinfI	GANTC	7 cut(s)	41, 70, 257, 322, 1091, 1603, 2345
HpaII	CCGG	3 cut(s)	580, 1468, 2372
HphI	GGTGA	3 cut(s)	1256, 1637, 2271
Hpy166II	GTNNAC	7 cut(s)	412, 646, 682, 1024, 2086, 2171, 2197
Hpy188I	TCNGA	9 cut(s)	75, 249, 703, 787, 1365, 1444, 1759, 2270, 2452
Hpy188III	TCNNGA	5 cut(s)	204, 437, 948, 1607, 2234
Hpy8I	GTNNAC	7 cut(s)	412, 646, 682, 1024, 2086, 2171, 2197
Hpy99I	CGWCG	1 cut(s)	117
HpyAV	CCTTC	9 cut(s)	335, 490, 913, 1439, 1534, 1850, 2210, 2275, 2332
HpyCH4III	ACNGT	8 cut(s)	214, 416, 643, 862, 1028, 1393, 1705, 2437
HpyCH4IV	ACGT	1 cut(s)	115
HpyF10VI	GCNNNNNNNGC	3 cut(s)	431, 440, 1062
HpyF3I	CTNAG	7 cut(s)	68, 248, 435, 784, 957, 1599, 1833
HpySE526I	ACGT	1 cut(s)	115
Hsp92I	GRCGYC	1 cut(s)	1888
HspAI	GCGC	2 cut(s)	362, 1711
LguI	GCTCTTC	1 cut(s)	1728
LmnI	GCTCC	6 cut(s)	160, 268, 313, 670, 751, 883
Lsp1109I	GCAGC	4 cut(s)	443, 674, 2022, 2363
LweI	GCATC	6 cut(s)	804, 908, 1321, 1338, 1407, 2143
MabI	ACCWGGT	1 cut(s)	1899
MaeI	CTAG	3 cut(s)	995, 2057, 2144
MaeII	ACGT	1 cut(s)	115
MaeIII	GTNAC	7 cut(s)	79, 916, 1037, 1243, 1625, 1796, 2114
MfeI	CAATTG	1 cut(s)	1617
MflI	RGATCY	4 cut(s)	688, 944, 2053, 2393
MhlI	GDGCHC	1 cut(s)	427
MlsI	TGGCCA	1 cut(s)	1481
MluNI	TGGCCA	1 cut(s)	1481
MlyI	GAGTC	6 cut(s)	50, 79, 331, 1100, 1612, 2339
MmeI	TCCRAC	5 cut(s)	110, 138, 433, 751, 1212
Mox20I	TGGCCA	1 cut(s)	1481
Mph1103I	ATGCAT	1 cut(s)	901
MroXI	GAANNNNTTC	1 cut(s)	256
MscI	TGGCCA	1 cut(s)	1481
MslI	CAYNNNNRTG	3 cut(s)	1029, 1266, 1277
Msp20I	TGGCCA	1 cut(s)	1481
MspA1I	CMGCKG	1 cut(s)	868
MspCI	CTTAAG	1 cut(s)	1192
MspI	CCGG	3 cut(s)	580, 1468, 2372
MspR9I	CCNGG	7 cut(s)	135, 1422, 1613, 1901, 2124, 2226, 2421
MunI	CAATTG	1 cut(s)	1617
Mva1269I	GAATGC	2 cut(s)	886, 1640
MvaI	CCWGG	7 cut(s)	135, 1422, 1613, 1901, 2124, 2226, 2421
MvnI	CGCG	3 cut(s)	362, 1713, 2287
MwoI	GCNNNNNNNGC	3 cut(s)	431, 440, 1062
NcoI	CCATGG	1 cut(s)	1267
NdeI	CATATG	1 cut(s)	670
NlaIV	GGNNCC	9 cut(s)	270, 425, 426, 648, 690, 747, 1898, 2232, 2395
NmeAIII	GCCGAG	1 cut(s)	2327
NmuCI	GTSAC	3 cut(s)	916, 1625, 2114
NsiI	ATGCAT	1 cut(s)	901
NspI	RCATGY	2 cut(s)	1678, 2295
PaeR7I	CTCGAG	1 cut(s)	202
PagI	TCATGA	1 cut(s)	1606
PciSI	GCTCTTC	1 cut(s)	1728
PctI	GAATGC	2 cut(s)	886, 1640
PdmI	GAANNNNTTC	1 cut(s)	256
PfeI	GAWTC	1 cut(s)	257
PflFI	GACNNNGTC	1 cut(s)	1428
PflMI	CCANNNNNTGG	2 cut(s)	674, 871
PfoI	TCCNGGA	2 cut(s)	133, 2419
PkrI	GCNGC	5 cut(s)	433, 479, 664, 2012, 2353
Ple19I	CGATCG	1 cut(s)	727
PleI	GAGTC	6 cut(s)	49, 78, 330, 1099, 1611, 2339
PpsI	GAGTC	6 cut(s)	49, 78, 330, 1099, 1611, 2339
PpuMI	RGGWCCY	1 cut(s)	961
PsiI	TTATAA	1 cut(s)	1773
Psp5II	RGGWCCY	1 cut(s)	961
Psp6I	CCWGG	7 cut(s)	133, 1420, 1611, 1899, 2122, 2224, 2419
PspEI	GGTNACC	1 cut(s)	1796
PspGI	CCWGG	7 cut(s)	133, 1420, 1611, 1899, 2122, 2224, 2419
PspN4I	GGNNCC	9 cut(s)	270, 425, 426, 648, 690, 747, 1898, 2232, 2395
PspOMI	GGGCCC	1 cut(s)	423
PspPI	GGNCC	7 cut(s)	423, 424, 499, 646, 763, 961, 2417
PspPPI	RGGWCCY	1 cut(s)	961
PstI	CTGCAG	1 cut(s)	433
PsuI	RGATCY	4 cut(s)	688, 944, 2053, 2393
PsyI	GACNNNGTC	1 cut(s)	1428
PvuI	CGATCG	1 cut(s)	727
PvuII	CAGCTG	1 cut(s)	868
RsaI	GTAC	8 cut(s)	413, 683, 722, 1307, 1667, 2102, 2132, 2274
RsaNI	GTAC	8 cut(s)	412, 682, 721, 1306, 1666, 2101, 2131, 2273
RseI	CAYNNNNRTG	3 cut(s)	1029, 1266, 1277
SapI	GCTCTTC	1 cut(s)	1728
SatI	GCNGC	5 cut(s)	432, 478, 663, 2011, 2352
Sau96I	GGNCC	7 cut(s)	423, 424, 499, 646, 763, 961, 2417
ScaI	AGTACT	2 cut(s)	1307, 2274
SchI	GAGTC	6 cut(s)	50, 79, 331, 1100, 1612, 2339
ScrFI	CCNGG	7 cut(s)	135, 1422, 1613, 1901, 2124, 2226, 2421
SduI	GDGCHC	1 cut(s)	427
SexAI	ACCWGGT	1 cut(s)	1899
SfaNI	GCATC	6 cut(s)	804, 908, 1321, 1338, 1407, 2143
SfcI	CTRYAG	1 cut(s)	429
Sfr274I	CTCGAG	1 cut(s)	202
SinI	GGWCC	4 cut(s)	499, 646, 961, 2417
SlaI	CTCGAG	1 cut(s)	202
SmiMI	CAYNNNNRTG	3 cut(s)	1029, 1266, 1277
SmlI	CTYRAG	2 cut(s)	202, 1192
SmoI	CTYRAG	2 cut(s)	202, 1192
SsiI	CCGC	5 cut(s)	477, 793, 1174, 1713, 2285
SspI	AATATT	1 cut(s)	1377
SspMI	CTAG	3 cut(s)	995, 2057, 2144
StyD4I	CCNGG	7 cut(s)	133, 1420, 1611, 1899, 2122, 2224, 2419
StyI	CCWWGG	4 cut(s)	57, 741, 994, 1267
TaaI	ACNGT	8 cut(s)	214, 416, 643, 862, 1028, 1393, 1705, 2437
TaiI	ACGT	1 cut(s)	118
TaqI	TCGA	7 cut(s)	112, 203, 295, 1231, 1535, 1842, 2308
TatI	WGTACW	5 cut(s)	681, 1305, 1665, 2130, 2272
TauI	GCSGC	1 cut(s)	480
TfiI	GAWTC	1 cut(s)	257
TscAI	CASTG	4 cut(s)	223, 646, 1054, 1318
TseFI	GTSAC	3 cut(s)	916, 1625, 2114
TseI	GCWGC	4 cut(s)	431, 662, 2010, 2351
Tsp45I	GTSAC	3 cut(s)	916, 1625, 2114
TspGWI	ACGGA	1 cut(s)	2386
TspRI	CASTG	4 cut(s)	223, 646, 1054, 1318
Tth111I	GACNNNGTC	1 cut(s)	1428
Van91I	CCANNNNNTGG	2 cut(s)	674, 871
Vha464I	CTTAAG	1 cut(s)	1192
VpaK11BI	GGWCC	4 cut(s)	499, 646, 961, 2417
XagI	CCTNNNNNAGG	1 cut(s)	135
XapI	RAATTY	8 cut(s)	356, 377, 575, 812, 1545, 1973, 2162, 2242
XceI	RCATGY	2 cut(s)	1678, 2295
XhoI	CTCGAG	1 cut(s)	202
XmaJI	CCTAGG	1 cut(s)	994
XmiI	GTMKAC	1 cut(s)	2196
XmnI	GAANNNNTTC	1 cut(s)	256
XspI	CTAG	3 cut(s)	995, 2057, 2144
ZrmI	AGTACT	2 cut(s)	1307, 2274
Zsp2I	ATGCAT	1 cut(s)	901

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.