Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0013627

Physical Location & Seq

Genomic Coordinates Forward (+)

5858728 .. 5864085

Gene Structure 5358 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000010992

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2130 bp

ATGTTGGGTTCTTTGGGTCTTCATCTCACTTGTTCTTTTGGGTCTTTATCGTGTTCCAAGGACTACCTGAGTCCGAGGGTAACTCAAACTAAAGCAGTCTCTTTTCAGCTTCGACATGGAGTATTGTCCCCTCCAGGAGGTTTAAGATGTGGGAAGTTGGAGCGTAGTCCCAGAGTTTCAGCAGTTTTGACAGACGACAGCTCGACAATGTCAGTCAGTGATGAAGAGATGGAAGATATTGGTGTTCTGAGAACAGATTCAAACTTGGAGCCATACAAAGATCATTTCAAGTATCGAATAAAAAGATATTTGGAGCAGAAGAGTCTCTTTGAGAAATATGAAGGAAGTTTAGAGGAATTCGCGCAAGGTTATGTGAAATTTGGATTTAACAGAGAAGATGGTGGAATTGTGTACCGTGAATGGGCCCCTGCAGCTCAGGAGGCACAACTTGTTGGAGAATTTAATGGATGGGATGGCTGCAAACACGAGATGGAGAAGGGCCAATTTGGGGTATGGAGTATTAAGATACCCGATTTTGGTGGAAGTCCAGCTATTCCTCACAATTCAAAGGTTAAATTCCGGTTCAAGCATGGAAATGGAGTTTGGGTTGATCGTATTCCTGCTTGGATTAAATATGCCACTGTGGACCCAACAAGATTTGCAGCTCCATATGATGGTGTGTACTGGGATCCACCACCATCAGAAAGGTATCAGTTTAAGTACGGTCGTCCTCCAAAACCCAAGGCTCCACGAATATATGAGGCCCATGTTGGAATGAGTAGCTCAGAACCCCGCATCAGTTCATACAGAGAATTTGCTGATAATGTTTTGCCTCGTATCCAAGCAAATAACTATAACACGGTCCAGCTGATGGCCGTGATGGAGCATTCCTATTATGCATCCTTCGGTTATCATGTGACAAACTTCTTTGCCGTAAGCAGTAGATCTGGAACACCTGAGGACCTTAAATATCTAATTGATAAAGCACATTCCCTAGGTTTGCGTGTGTTGATGGATGTTGTTCACAGTCATGCAAGTAATAACGTCACTGATGGCCTCAGTGGCTTTGAAGTTGGTCAATGCACACAAGATTCCTATTTCCACACAGGAGATAGAGGCTACCATAAGCTATGGGATAGCCGACTTTTCAACTATGCTAATTGGGAAGTTCTCCGCTTCTTTTTATCCAACTTAAGGTGGTGGCTGGAGGAGTATCAATTTGATGGGTTTCGATTTGATGGAGTAACTTCCATGCTGTATCATCACCATGGCATAAATATGGCATTTACAGGGAATTATGATGAGTACTTCAGTGAGGCAACAGATGTTGATGCTGTTGTTTATCTGATGCTTGCCAACTATCTGATCCATAAAATATTGCCATCTGCAACTGTAATTGCTGAAGATGTTTCTGGGATGCCTGGACTTGGTCGGCCTGTCTCTGAAGGAGGTGTTGGTTTTGACTACCGGCTGGCAATGGCCATCCCTGACAAGTGGATTGATTATGTGAAAAACAAGAAAGACGAAGAGTGGTCGATGAAGGAAATTTCATGGAGCTTGACGAATAGGAGATACACCGAGAAGTGTGTTTCCTATGCTGAGAGTCATGACCAGGCAATTGTGGGTGACAAGACGATTGCATTCTTTTTGATGGATAAAGAAATGTACTCTGGCATGTCTTGTTTGAATGAAGCATCTCCTACTGTTGAGCGCGGGGTTGCTCTTCACAAGATGATACATTTTATAACTATGGCATTAGGAGGCGAGGGTTACCTTAACTTTATGGGTAATGAGTTTGGACACCCTGAGTGGATCGACTTTCCAAGAGAAGGCAATGGGTGGAGTTATGAAAAGTGCAGACGCCAATGGAACCTGGTGGATACAGATCATTTAAGATATAAGTTTATGAACGCATTTGACAGAGCTATGCACTTGCTTGATGAGAAATTTTTGTTCCTTTCATCAACGAAGCAGATAGTGAGCAGCACAAATGAAGAAGACAAGGTTATTGTCTTTGAGCGTGGAGATCTAGTTTTTGTATTTAATTTTCATCCAGTAAACACATACGATGGGTATATTTCCTTTTGCTTATTTGTTGATGTAGCTGCATGCTTGATAGAAGCAGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003844 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0006073 GO:0008150 GO:0008152 GO:0009501 GO:0009507 GO:0009536 GO:0009987 GO:0016740 GO:0016757 GO:0016758 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase Alpha-amylase_C CBM_48

Protein Analysis

710

Amino Acids

81.19

Weight (kDa)

5.79

Isoelectric Point (pI)

34.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_48	PF02922	126 - 209	1.1e-19	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Alpha-amylase	PF00128	299 - 349	5e-09	Alpha amylase, catalytic domain
Alpha-amylase_C	PF02806	661 - 692	3.3e-06	Alpha amylase, C-terminal all-beta domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013627)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g37381 FvH4_2g37410
malus_domestica	MD08G1002300.v1.1
prunus_persica	Prupe.1G354000_v2.0.a1 Prupe.1G354000_v2.0.a1 Prupe.1G354000_v2.0.a1
pyrus_communis	pycom08g00150
rosa_chinensis	RchiOBHm_Chr6g0304311
rosa_laevigata	RLG00000010992
rosa_multiflora	Rmu_ssc0000218.1_g000037
rosa_roxburghii	Rroxscaffold_7G00163850
rosa_rugosa	Rorug06G0329900 Rorug06G0330000
rosa_samantha	Rh6AG443300 Rh6BG459100 Rh6CG456100 Rh6DG442800
rosa_wichuraiana	Rw6G038470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1745
AccB7I	CCANNNNNTGG	2 cut(s)	674, 871
AccII	CGCG	2 cut(s)	362, 1713
AciI	CCGC	3 cut(s)	793, 1174, 1713
AclWI	GGATC	4 cut(s)	683, 696, 1360, 1820
AcoI	YGGCCR	2 cut(s)	873, 1479
AcsI	RAATTY	7 cut(s)	356, 377, 458, 575, 812, 1545, 1946
AcuI	CTGAAG	3 cut(s)	1294, 1422, 1464
AcyI	GRCGYC	1 cut(s)	1861
AfaI	GTAC	5 cut(s)	413, 683, 722, 1307, 1667
AfiI	CCNNNNNNNGG	9 cut(s)	137, 421, 508, 536, 674, 871, 1194, 1427, 1829
AflII	CTTAAG	1 cut(s)	1192
AgsI	TTSAA	7 cut(s)	261, 289, 567, 586, 1070, 1150, 1687
AjnI	CCWGG	4 cut(s)	133, 1420, 1611, 1872
AjuI	GAANNNNNNNTTGG	4 cut(s)	586, 618, 1437, 1469
Alw26I	GTCTC	3 cut(s)	103, 329, 1444
AlwI	GGATC	4 cut(s)	683, 696, 1360, 1820
AoxI	GGCC	7 cut(s)	423, 499, 762, 873, 1054, 1433, 1479
ApaI	GGGCCC	1 cut(s)	427
ApeKI	GCWGC	5 cut(s)	431, 477, 662, 1983, 2105
ApoI	RAATTY	7 cut(s)	356, 377, 458, 575, 812, 1545, 1946
Asp700I	GAANNNNTTC	1 cut(s)	256
AspA2I	CCTAGG	1 cut(s)	994
AspLEI	GCGC	2 cut(s)	364, 1713
AspS9I	GGNCC	7 cut(s)	423, 424, 499, 646, 763, 862, 961
AsuHPI	GGTGA	2 cut(s)	1256, 1637
AvaII	GGWCC	3 cut(s)	646, 862, 961
AvrII	CCTAGG	1 cut(s)	994
AxyI	CCTNAGG	1 cut(s)	957
BaeGI	GKGCMC	1 cut(s)	427
BalI	TGGCCA	1 cut(s)	1481
BamHI	GGATCC	1 cut(s)	688
BanII	GRGCYC	1 cut(s)	427
BauI	CACGAG	1 cut(s)	485
BbsI	GAAGAC	2 cut(s)	11, 2004
BbvI	GCAGC	5 cut(s)	443, 464, 674, 1995, 2092
BceAI	ACGGC	2 cut(s)	860, 917
BciT130I	CCWGG	4 cut(s)	135, 1422, 1613, 1874
BciVI	GTATCC	2 cut(s)	848, 1873
BcoDI	GTCTC	3 cut(s)	103, 329, 1444
BfaI	CTAG	2 cut(s)	995, 2030
BfmI	CTRYAG	1 cut(s)	429
BfrI	CTTAAG	1 cut(s)	1192
BfuI	GTATCC	2 cut(s)	848, 1873
BglI	GCCNNNNNGGC	1 cut(s)	1062
BglII	AGATCT	2 cut(s)	944, 2026
BisI	GCNGC	5 cut(s)	432, 478, 663, 1984, 2106
BlnI	CCTAGG	1 cut(s)	994
BlsI	GCNGC	5 cut(s)	433, 479, 664, 1985, 2107
BmcAI	AGTACT	1 cut(s)	1307
Bme1390I	CCNGG	4 cut(s)	135, 1422, 1613, 1874
Bme18I	GGWCC	3 cut(s)	646, 862, 961
BmgT120I	GGNCC	7 cut(s)	423, 424, 499, 646, 763, 862, 961
BmiI	GGNNCC	7 cut(s)	270, 425, 426, 648, 690, 747, 1871
BmrFI	CCNGG	4 cut(s)	135, 1422, 1613, 1874
BmrI	ACTGGG	1 cut(s)	694
BmsI	GCATC	6 cut(s)	804, 908, 1321, 1338, 1407, 1703
BmuI	ACTGGG	1 cut(s)	694
BpiI	GAAGAC	2 cut(s)	11, 2004
BplI	GAGNNNNNCTC	2 cut(s)	67, 99
BpmI	CTGGAG	2 cut(s)	117, 1226
Bpu10I	CCTNAGC	1 cut(s)	435
BsaBI	GATNNNNATC	1 cut(s)	1884
BsaHI	GRCGYC	1 cut(s)	1861
BsaJI	CCNNGG	5 cut(s)	57, 74, 741, 994, 1267
BsaWI	WCCGGW	1 cut(s)	579
BsaXI	ACNNNNNCTCC	4 cut(s)	591, 621, 2016, 2046
Bsc4I	CCNNNNNNNGG	9 cut(s)	137, 421, 508, 536, 674, 871, 1194, 1427, 1829
Bse118I	RCCGGY	1 cut(s)	1467
Bse1I	ACTGG	2 cut(s)	689, 2054
Bse21I	CCTNAGG	1 cut(s)	957
Bse3DI	GCAATG	2 cut(s)	1482, 1840
Bse8I	GATNNNNATC	1 cut(s)	1884
BseBI	CCWGG	4 cut(s)	135, 1422, 1613, 1874
BseDI	CCNNGG	5 cut(s)	57, 74, 741, 994, 1267
BseGI	GGATG	7 cut(s)	473, 478, 899, 1021, 1422, 1482, 2050
BseJI	GATNNNNATC	1 cut(s)	1884
BseLI	CCNNNNNNNGG	9 cut(s)	137, 421, 508, 536, 674, 871, 1194, 1427, 1829
BseMI	GCAATG	2 cut(s)	1482, 1840
BseMII	CTCAG	8 cut(s)	59, 239, 449, 798, 948, 1072, 1590, 1797
BseNI	ACTGG	2 cut(s)	689, 2054
BseRI	GAGGAG	1 cut(s)	1223
BseSI	GKGCMC	1 cut(s)	427
BseXI	GCAGC	5 cut(s)	443, 464, 674, 1995, 2092
BsgI	GTGCAG	1 cut(s)	1876
Bsh1236I	CGCG	2 cut(s)	362, 1713
Bsh1285I	CGRYCG	1 cut(s)	727
BshFI	GGCC	7 cut(s)	425, 501, 764, 875, 1056, 1435, 1481
BsiEI	CGRYCG	1 cut(s)	727
BsiSI	CCGG	2 cut(s)	580, 1468
BslFI	GGGAC	2 cut(s)	112, 153
BslI	CCNNNNNNNGG	9 cut(s)	137, 421, 508, 536, 674, 871, 1194, 1427, 1829
BsmAI	GTCTC	3 cut(s)	103, 329, 1444
BsmFI	GGGAC	2 cut(s)	112, 153
BsmI	GAATGC	2 cut(s)	886, 1640
BsnI	GGCC	7 cut(s)	425, 501, 764, 875, 1056, 1435, 1481
Bsp120I	GGGCCC	1 cut(s)	423
Bsp1286I	GDGCHC	1 cut(s)	427
Bsp143I	GATC	8 cut(s)	280, 610, 688, 944, 1365, 1812, 1885, 2026
Bsp19I	CCATGG	1 cut(s)	1267
BspACI	CCGC	3 cut(s)	793, 1174, 1713
BspANI	GGCC	7 cut(s)	425, 501, 764, 875, 1056, 1435, 1481
BspCNI	CTCAG	8 cut(s)	60, 240, 448, 797, 949, 1071, 1591, 1798
BspFNI	CGCG	2 cut(s)	362, 1713
BspHI	TCATGA	1 cut(s)	1606
BspLI	GGNNCC	7 cut(s)	270, 425, 426, 648, 690, 747, 1871
BspMAI	CTGCAG	1 cut(s)	433
BspPI	GGATC	4 cut(s)	683, 696, 1360, 1820
BspQI	GCTCTTC	1 cut(s)	1728
BspTI	CTTAAG	1 cut(s)	1192
BsrDI	GCAATG	2 cut(s)	1482, 1840
BsrFI	RCCGGY	1 cut(s)	1467
BsrI	ACTGG	2 cut(s)	689, 2054
BssAI	RCCGGY	1 cut(s)	1467
BssECI	CCNNGG	5 cut(s)	57, 74, 741, 994, 1267
BssMI	GATC	8 cut(s)	280, 610, 688, 944, 1365, 1812, 1885, 2026
BssNI	GRCGYC	1 cut(s)	1861
BssSI	CACGAG	1 cut(s)	485
BssT1I	CCWWGG	4 cut(s)	57, 741, 994, 1267
Bst2BI	CACGAG	1 cut(s)	485
Bst2UI	CCWGG	4 cut(s)	135, 1422, 1613, 1874
Bst4CI	ACNGT	7 cut(s)	416, 643, 725, 862, 1028, 1393, 1705
Bst6I	CTCTTC	4 cut(s)	219, 314, 1521, 1728
BstACI	GRCGYC	1 cut(s)	1861
BstAFI	CTTAAG	1 cut(s)	1192
BstC8I	GCNNGC	3 cut(s)	1353, 1473, 2110
BstDEI	CTNAG	8 cut(s)	68, 248, 435, 784, 957, 1058, 1599, 1806
BstDSI	CCRYGG	1 cut(s)	1267
BstEII	GGTNACC	1 cut(s)	1769
BstENI	CCTNNNNNAGG	1 cut(s)	135
BstF5I	GGATG	7 cut(s)	473, 478, 899, 1021, 1422, 1482, 2050
BstFNI	CGCG	2 cut(s)	362, 1713
BstHHI	GCGC	2 cut(s)	364, 1713
BstKTI	GATC	8 cut(s)	283, 613, 691, 947, 1368, 1815, 1888, 2029
BstMAI	GTCTC	3 cut(s)	103, 329, 1444
BstMBI	GATC	8 cut(s)	280, 610, 688, 944, 1365, 1812, 1885, 2026
BstMCI	CGRYCG	1 cut(s)	727
BstMWI	GCNNNNNNNGC	3 cut(s)	431, 440, 1062
BstNI	CCWGG	4 cut(s)	135, 1422, 1613, 1874
BstNSI	RCATGY	2 cut(s)	1678, 2112
BstPI	GGTNACC	1 cut(s)	1769
BstSCI	CCNGG	4 cut(s)	133, 1420, 1611, 1872
BstSFI	CTRYAG	1 cut(s)	429
BstSLI	GKGCMC	1 cut(s)	427
BstUI	CGCG	2 cut(s)	362, 1713
BstV1I	GCAGC	5 cut(s)	443, 464, 674, 1995, 2092
BstV2I	GAAGAC	2 cut(s)	11, 2004
BstX2I	RGATCY	3 cut(s)	688, 944, 2026
BstYI	RGATCY	3 cut(s)	688, 944, 2026
Bsu36I	CCTNAGG	1 cut(s)	957
BsuI	GTATCC	2 cut(s)	848, 1873
BsuRI	GGCC	7 cut(s)	425, 501, 764, 875, 1056, 1435, 1481
BtgI	CCRYGG	1 cut(s)	1267
BtsCI	GGATG	7 cut(s)	473, 478, 899, 1021, 1422, 1482, 2050
BtsIMutI	CAGTG	5 cut(s)	223, 639, 1047, 1066, 1318
Cac8I	GCNNGC	3 cut(s)	1353, 1473, 2110
CciI	TCATGA	1 cut(s)	1606
CfoI	GCGC	2 cut(s)	364, 1713
Cfr10I	RCCGGY	1 cut(s)	1467
Cfr13I	GGNCC	7 cut(s)	423, 424, 499, 646, 763, 862, 961
CseI	GACGC	1 cut(s)	1869
CsiI	ACCWGGT	1 cut(s)	1872
Csp6I	GTAC	5 cut(s)	412, 682, 721, 1306, 1666
CviQI	GTAC	5 cut(s)	412, 682, 721, 1306, 1666
DdeI	CTNAG	8 cut(s)	68, 248, 435, 784, 957, 1058, 1599, 1806
DpnI	GATC	8 cut(s)	282, 612, 690, 946, 1367, 1814, 1887, 2028
DpnII	GATC	8 cut(s)	280, 610, 688, 944, 1365, 1812, 1885, 2026
EaeI	YGGCCR	2 cut(s)	873, 1479
Eam1104I	CTCTTC	4 cut(s)	219, 314, 1521, 1728
EarI	CTCTTC	4 cut(s)	219, 314, 1521, 1728
Eco130I	CCWWGG	4 cut(s)	57, 741, 994, 1267
Eco24I	GRGCYC	1 cut(s)	427
Eco47I	GGWCC	3 cut(s)	646, 862, 961
Eco57I	CTGAAG	3 cut(s)	1294, 1422, 1464
Eco81I	CCTNAGG	1 cut(s)	957
Eco91I	GGTNACC	1 cut(s)	1769
EcoNI	CCTNNNNNAGG	1 cut(s)	135
EcoO109I	RGGNCCY	2 cut(s)	424, 961
EcoO65I	GGTNACC	1 cut(s)	1769
EcoRI	GAATTC	1 cut(s)	356
EcoRII	CCWGG	4 cut(s)	133, 1420, 1611, 1872
EcoT14I	CCWWGG	4 cut(s)	57, 741, 994, 1267
EcoT22I	ATGCAT	1 cut(s)	901
EcoT38I	GRGCYC	1 cut(s)	427
ErhI	CCWWGG	4 cut(s)	57, 741, 994, 1267
FalI	AAGNNNNNCTT	2 cut(s)	311, 343
FaqI	GGGAC	2 cut(s)	112, 153
FauI	CCCGC	2 cut(s)	800, 1706
FauNDI	CATATG	1 cut(s)	670
Fnu4HI	GCNGC	5 cut(s)	432, 478, 663, 1984, 2106
FokI	GGATG	7 cut(s)	480, 485, 886, 1028, 1429, 1469, 2037
FriOI	GRGCYC	1 cut(s)	427
Fsp4HI	GCNGC	5 cut(s)	432, 478, 663, 1984, 2106
FspBI	CTAG	2 cut(s)	995, 2030
GlaI	GCGC	2 cut(s)	363, 1712
GluI	GCNGC	5 cut(s)	432, 478, 663, 1984, 2106
GsuI	CTGGAG	2 cut(s)	117, 1226
HaeIII	GGCC	7 cut(s)	425, 501, 764, 875, 1056, 1435, 1481
HapII	CCGG	2 cut(s)	580, 1468
HgaI	GACGC	1 cut(s)	1869
HhaI	GCGC	2 cut(s)	364, 1713
Hin1I	GRCGYC	1 cut(s)	1861
Hin6I	GCGC	2 cut(s)	362, 1711
HinP1I	GCGC	2 cut(s)	362, 1711
HinfI	GANTC	5 cut(s)	70, 257, 322, 1091, 1603
HpaII	CCGG	2 cut(s)	580, 1468
HphI	GGTGA	2 cut(s)	1256, 1637
Hpy166II	GTNNAC	5 cut(s)	412, 646, 682, 1024, 2059
Hpy188I	TCNGA	7 cut(s)	75, 249, 703, 787, 1347, 1365, 1444
Hpy188III	TCNNGA	3 cut(s)	437, 948, 1607
Hpy8I	GTNNAC	5 cut(s)	412, 646, 682, 1024, 2059
HpyAV	CCTTC	6 cut(s)	335, 490, 913, 1439, 1534, 1823
HpyCH4III	ACNGT	7 cut(s)	416, 643, 725, 862, 1028, 1393, 1705
HpyCH4IV	ACGT	1 cut(s)	1044
HpyF10VI	GCNNNNNNNGC	3 cut(s)	431, 440, 1062
HpyF3I	CTNAG	8 cut(s)	68, 248, 435, 784, 957, 1058, 1599, 1806
HpySE526I	ACGT	1 cut(s)	1044
Hsp92I	GRCGYC	1 cut(s)	1861
HspAI	GCGC	2 cut(s)	362, 1711
Kzo9I	GATC	8 cut(s)	280, 610, 688, 944, 1365, 1812, 1885, 2026
LguI	GCTCTTC	1 cut(s)	1728
LmnI	GCTCC	7 cut(s)	160, 268, 313, 670, 751, 883, 1554
Lsp1109I	GCAGC	5 cut(s)	443, 464, 674, 1995, 2092
LweI	GCATC	6 cut(s)	804, 908, 1321, 1338, 1407, 1703
MabI	ACCWGGT	1 cut(s)	1872
MaeI	CTAG	2 cut(s)	995, 2030
MaeII	ACGT	1 cut(s)	1044
MaeIII	GTNAC	6 cut(s)	79, 916, 1045, 1243, 1625, 1769
MalI	GATC	8 cut(s)	282, 612, 690, 946, 1367, 1814, 1887, 2028
MboI	GATC	8 cut(s)	280, 610, 688, 944, 1365, 1812, 1885, 2026
MfeI	CAATTG	1 cut(s)	1617
MflI	RGATCY	3 cut(s)	688, 944, 2026
MhlI	GDGCHC	1 cut(s)	427
MlsI	TGGCCA	1 cut(s)	1481
MluNI	TGGCCA	1 cut(s)	1481
MlyI	GAGTC	3 cut(s)	79, 331, 1612
MmeI	TCCRAC	4 cut(s)	138, 433, 751, 1212
Mox20I	TGGCCA	1 cut(s)	1481
Mph1103I	ATGCAT	1 cut(s)	901
MroXI	GAANNNNTTC	1 cut(s)	256
MscI	TGGCCA	1 cut(s)	1481
MslI	CAYNNNNRTG	4 cut(s)	594, 1029, 1266, 1277
Msp20I	TGGCCA	1 cut(s)	1481
MspA1I	CMGCKG	1 cut(s)	868
MspCI	CTTAAG	1 cut(s)	1192
MspI	CCGG	2 cut(s)	580, 1468
MspR9I	CCNGG	4 cut(s)	135, 1422, 1613, 1874
MunI	CAATTG	1 cut(s)	1617
Mva1269I	GAATGC	2 cut(s)	886, 1640
MvaI	CCWGG	4 cut(s)	135, 1422, 1613, 1874
MvnI	CGCG	2 cut(s)	362, 1713
MwoI	GCNNNNNNNGC	3 cut(s)	431, 440, 1062
NcoI	CCATGG	1 cut(s)	1267
NdeI	CATATG	1 cut(s)	670
NdeII	GATC	8 cut(s)	280, 610, 688, 944, 1365, 1812, 1885, 2026
NlaIV	GGNNCC	7 cut(s)	270, 425, 426, 648, 690, 747, 1871
NmuCI	GTSAC	3 cut(s)	916, 1045, 1625
NsiI	ATGCAT	1 cut(s)	901
NspI	RCATGY	2 cut(s)	1678, 2112
PaeI	GCATGC	1 cut(s)	2112
PagI	TCATGA	1 cut(s)	1606
PciSI	GCTCTTC	1 cut(s)	1728
PctI	GAATGC	2 cut(s)	886, 1640
PdmI	GAANNNNTTC	1 cut(s)	256
PfeI	GAWTC	2 cut(s)	257, 1091
PflFI	GACNNNGTC	2 cut(s)	208, 1428
PflMI	CCANNNNNTGG	2 cut(s)	674, 871
PfoI	TCCNGGA	1 cut(s)	133
PkrI	GCNGC	5 cut(s)	433, 479, 664, 1985, 2107
PleI	GAGTC	3 cut(s)	78, 330, 1611
PpsI	GAGTC	3 cut(s)	78, 330, 1611
PpuMI	RGGWCCY	1 cut(s)	961
PsiI	TTATAA	1 cut(s)	1745
Psp5II	RGGWCCY	1 cut(s)	961
Psp6I	CCWGG	4 cut(s)	133, 1420, 1611, 1872
PspEI	GGTNACC	1 cut(s)	1769
PspGI	CCWGG	4 cut(s)	133, 1420, 1611, 1872
PspN4I	GGNNCC	7 cut(s)	270, 425, 426, 648, 690, 747, 1871
PspOMI	GGGCCC	1 cut(s)	423
PspPI	GGNCC	7 cut(s)	423, 424, 499, 646, 763, 862, 961
PspPPI	RGGWCCY	1 cut(s)	961
PstI	CTGCAG	1 cut(s)	433
PsuI	RGATCY	3 cut(s)	688, 944, 2026
PsyI	GACNNNGTC	2 cut(s)	208, 1428
PvuII	CAGCTG	1 cut(s)	868
RsaI	GTAC	5 cut(s)	413, 683, 722, 1307, 1667
RsaNI	GTAC	5 cut(s)	412, 682, 721, 1306, 1666
RseI	CAYNNNNRTG	4 cut(s)	594, 1029, 1266, 1277
SapI	GCTCTTC	1 cut(s)	1728
SatI	GCNGC	5 cut(s)	432, 478, 663, 1984, 2106
Sau3AI	GATC	8 cut(s)	280, 610, 688, 944, 1365, 1812, 1885, 2026
Sau96I	GGNCC	7 cut(s)	423, 424, 499, 646, 763, 862, 961
ScaI	AGTACT	1 cut(s)	1307
SchI	GAGTC	3 cut(s)	79, 331, 1612
ScrFI	CCNGG	4 cut(s)	135, 1422, 1613, 1874
SduI	GDGCHC	1 cut(s)	427
SexAI	ACCWGGT	1 cut(s)	1872
SfaNI	GCATC	6 cut(s)	804, 908, 1321, 1338, 1407, 1703
SfcI	CTRYAG	1 cut(s)	429
SinI	GGWCC	3 cut(s)	646, 862, 961
SmiMI	CAYNNNNRTG	4 cut(s)	594, 1029, 1266, 1277
SmlI	CTYRAG	1 cut(s)	1192
SmoI	CTYRAG	1 cut(s)	1192
SphI	GCATGC	1 cut(s)	2112
SsiI	CCGC	3 cut(s)	793, 1174, 1713
SspI	AATATT	1 cut(s)	1377
SspMI	CTAG	2 cut(s)	995, 2030
StyD4I	CCNGG	4 cut(s)	133, 1420, 1611, 1872
StyI	CCWWGG	4 cut(s)	57, 741, 994, 1267
TaaI	ACNGT	7 cut(s)	416, 643, 725, 862, 1028, 1393, 1705
TaiI	ACGT	1 cut(s)	1047
TaqI	TCGA	6 cut(s)	112, 203, 295, 1231, 1535, 1815
TatI	WGTACW	3 cut(s)	681, 1305, 1665
TfiI	GAWTC	2 cut(s)	257, 1091
TscAI	CASTG	5 cut(s)	223, 646, 1054, 1066, 1318
TseFI	GTSAC	3 cut(s)	916, 1045, 1625
TseI	GCWGC	5 cut(s)	431, 477, 662, 1983, 2105
Tsp45I	GTSAC	3 cut(s)	916, 1045, 1625
TspRI	CASTG	5 cut(s)	223, 646, 1054, 1066, 1318
Tth111I	GACNNNGTC	2 cut(s)	208, 1428
Van91I	CCANNNNNTGG	2 cut(s)	674, 871
Vha464I	CTTAAG	1 cut(s)	1192
VpaK11BI	GGWCC	3 cut(s)	646, 862, 961
XagI	CCTNNNNNAGG	1 cut(s)	135
XapI	RAATTY	7 cut(s)	356, 377, 458, 575, 812, 1545, 1946
XceI	RCATGY	2 cut(s)	1678, 2112
XmaJI	CCTAGG	1 cut(s)	994
XmnI	GAANNNNTTC	1 cut(s)	256
XspI	CTAG	2 cut(s)	995, 2030
ZrmI	AGTACT	1 cut(s)	1307
Zsp2I	ATGCAT	1 cut(s)	901

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.