FvH4_2g37410

1,4-alpha-glucan-branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0013627
Physical Location & Seq
Forward (+)
27274069 .. 27279002
4934 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g37410.t1

Sequence Viewer

Length: 2514 bp
ATGCTGGGCTCTTTGGGTCGTCTTAATCTCACTTATTCTTCTTCTTCTGGGTCTTTCTCGTCTTCCAAGGACTACCCGAGTCCGAGGCTATCTCATCCCAAACTACCCTCTTTTCCGCTGCTTCGACATAGAGTACTGTCCCCTCCAACTAATTTAAGATGTGGGAAGTTGGAGCGTGATTGCAAAGTTAGAGCAGTTTTGACAGACGACAGCTCGATAATGACGGTGAATGAAGGAGAGACGGAAGATATTGGTATTTTGAGAACAGACTCAAACTTGGAGCCGTACAAAGATCACTTCAAGTGTAGAATAAAGAGATATTTGGAGCAGAAAAGTCTCTTTGAGAAATATGAAGGAGGTCTAGAGGAATTCGCTCGAGGTTTTGTGAAATTTGGATTTAATAGAGAAGACGATGGAGTTGTGTACCGTGAATGGGCCCCTGCAGCTCAGGAGGCCCAACTTGTTGGAGACTTTAATGGATGGGAGGGCTCCAATCACAAGATGGAGAAGGGCGAGTTTGGAGTATGGAGTATCAAGATACATGACTTTGATGGAAATCCAGCTATTCCTCACAATTCAAAGGTTAAGTTCCGGTTCAAGCATGGAGATGGAGTTTGGGTTGATCGTATTCCTGCTTGGATTAAATATGCCACTGTGGATCCAGCAAAGTTTGCAGCTCCATATGATGGTGTGTACTGGGATCCACCACCATCAGAAAGGTATCAGTTTAAGTATGGTCGTCCTCCAAAACCCAAGGCTCCACGAATATATGAGGCCCATGTTGGAATGAGTAGCTCAGAACCCCGCATCAGTTCATACAGAGAATTTGCTGATAATGTTTTGCCTCGTATTCGAGCAAATAACTATAACACAGTCCAGCTGATGGCTGTGATGGAGCATTCCTATTATGCTTCCTTCGGTTATCATGTGACAAATTTCTTTGCCGTAAGCAGTAGATCTGGAACACCTGAGGACCTTAAATATCTAATTGATAAAGCACATTCCCTAGGTTTGCGGGTATTGATGGATGTCATTCACAGTCATGCAAGTAATAATGTCACTGATGGCCTCAATGGCTTTGAAGTTGGTCAAAGCACACAAGAGTCCTACTTCCATGCAGGAGATAGAGGCTACCATAAGTTATGGGATAGCCGACTCTTCAACTATGCAAACTGGGAAGTTCTCCGCTTCTTTTTGTCCAACTTAAGGTGGTGGCTGGAGGAGTATCAGTTTGATGGTTTTCGGTTCGATGGGGTAACTTCCATGCTGTATCATCACCATGGCATAAACGTGGCATTTACAGGAGATTATAATGAGTACTTCAGTGAGGCAACAGATGTTGATGCTGTTGTTTATTTGATGCTTGCCAACTATCTGATCCATAAGATACTGCCATCTGCAACTGTAATTGCCGAAGATGTTTCTGGGATGCCTGGACTTGGTCGGCCTGTCTCTGAAGGAGGTGTTGGTTTTGACTACAGGCTGGCAATGGCCATCCCTGATAAGTGGATTGATTATGTGAAAAACAAGAAAGACGAAGAGTGGTCAGTGACAGAAATTTCATGGAGCTTGACAAATAGGAGATACACCGAGAAATGTGTCTCCTATGCTGAGAGTCATGATCAGTCAATTGTGGGTGACAAAACGATTGCATTCTTTTTGATGGATAAAGAAATGTACTCTGGTATGTCGTGTTTGAAGGAAGCTTCTCCTACTGTTGATCGCGGGATTGCCCTCCACAAGATGATACATTTTATAACTATGGCATTAGGAGGCGAGGGCTATCTTAACTTTATGGGTAATGAGTTTGGACACCCTGAATGGATTGACTTTCCAAGAGATGGCAATGGGTGGAGTTATGAAAAGTGCAGACGCCAATGGAACCTGGTGGATACAGATCATTTAAGATATAAGTTTATGAACGCATTTGACAGAGCTATGCACTTGCTTGATGAGAAATTTTCGTTCCTTTCATCAACAAAGCAGATAGTGAGCAGCACAGATGAGGAAGACAAGGTTATTGTCTTTGAGCGTGGAGATCTAGTTTTTGTATTCAATTTTCATCCAGTAAACACATACGATGGGTACAAAGTGGGGTGTGACTTACCTGGGAAGTACAGAGTTGCACTAGACAGTGATGCTTTCGAATTTGGTGGACATGGAAGAGTAGGGCATGATGTAGACCACTTCACATTTCCAGAAGGAATACCAGGGGTTCCTGAAACAAATTTCAACGGCCGTCCCAACTCCTTCAAAGTACTCTCACCACCGCGAACATCCGTGGTTTATTACCGAGTGGATGAAAGTAAGGAGGAGAACTCAACAGCAGAAGTTGTGGTTGGGCAGGAGAACAAAGAAGTTGGATACATGAAACATGAAAATGTTGGTCCAGGAGTGAATTTGTTATCGGAGAACTCAACAGCAGAAGTTGTGGTTGGGCAGGAGAACAAAGAAGTTGGATCCATGAAACATGAAAATGTTGGTCCAGGAGTGAATTTGTCATCGGATAACTGA

Protein Analysis

838

Amino Acids

95.25

Weight (kDa)

5.73

Isoelectric Point (pI)

33.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBM_48 PF02922 130 - 213 1.7e-20 Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Alpha-amylase PF00128 303 - 370 7.7e-09 Alpha amylase, catalytic domain
Alpha-amylase_C PF02806 665 - 765 1.6e-20 Alpha amylase, C-terminal all-beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013627)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g37381 FvH4_2g37410
malus_domestica MD08G1002300.v1.1
prunus_persica Prupe.1G354000_v2.0.a1 Prupe.1G354000_v2.0.a1 Prupe.1G354000_v2.0.a1
pyrus_communis pycom08g00150
rosa_chinensis RchiOBHm_Chr6g0304311
rosa_laevigata RLG00000010992
rosa_multiflora Rmu_ssc0000218.1_g000037
rosa_roxburghii Rroxscaffold_7G00163850
rosa_rugosa Rorug06G0329900 Rorug06G0330000
rosa_samantha Rh6AG443300 Rh6BG459100 Rh6CG456100 Rh6DG442800
rosa_wichuraiana Rw6G038470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1311, 1757
AccB7I CCANNNNNTGG 3 cut(s) 686, 883, 1841
AccI GTMKAC 1 cut(s) 2181
AccII CGCG 2 cut(s) 1725, 2272
AciI CCGC 6 cut(s) 116, 805, 1015, 1186, 1725, 2270
AclWI GGATC 7 cut(s) 653, 666, 695, 708, 1372, 2454, 2467
AcoI YGGCCR 2 cut(s) 1491, 2236
AcuI CTGAAG 2 cut(s) 1306, 1476
AcyI GRCGYC 1 cut(s) 1873
AfaI GTAC 9 cut(s) 135, 287, 425, 695, 1319, 1679, 2087, 2117, 2259
AfiI CCNNNNNNNGG 6 cut(s) 433, 686, 883, 1206, 1439, 1841
AflII CTTAAG 1 cut(s) 1204
AgsI TTSAA 9 cut(s) 301, 579, 598, 1082, 1162, 1699, 2056, 2233, 2254
AjnI CCWGG 6 cut(s) 1432, 1884, 2107, 2209, 2389, 2485
AjuI GAANNNNNNNTTGG 6 cut(s) 1449, 1481, 2322, 2354, 2418, 2450
AluBI AGCT 9 cut(s) 213, 446, 563, 677, 795, 880, 1569, 1706, 1937
AluI AGCT 9 cut(s) 213, 446, 563, 677, 795, 880, 1569, 1706, 1937
Alw26I GTCTC 5 cut(s) 233, 341, 462, 1456, 1606
AlwI GGATC 7 cut(s) 653, 666, 695, 708, 1372, 2454, 2467
Ama87I CYCGRG 2 cut(s) 76, 375
AoxI GGCC 7 cut(s) 435, 453, 774, 1066, 1445, 1491, 2236
ApaI GGGCCC 1 cut(s) 439
ApeKI GCWGC 4 cut(s) 118, 443, 674, 1995
AspA2I CCTAGG 1 cut(s) 1006
AspS9I GGNCC 7 cut(s) 435, 436, 454, 775, 973, 2387, 2483
AsuHPI GGTGA 4 cut(s) 238, 1268, 1649, 2256
AsuII TTCGAA 1 cut(s) 2145
AvaI CYCGRG 2 cut(s) 76, 375
AvaII GGWCC 3 cut(s) 973, 2387, 2483
AvrII CCTAGG 1 cut(s) 1006
AxyI CCTNAGG 1 cut(s) 969
BaeGI GKGCMC 1 cut(s) 439
BalI TGGCCA 1 cut(s) 1493
BamHI GGATCC 3 cut(s) 658, 700, 2459
BanII GRGCYC 3 cut(s) 11, 439, 491
BbsI GAAGAC 3 cut(s) 54, 414, 2016
BbvI GCAGC 4 cut(s) 105, 455, 686, 2007
BceAI ACGGC 4 cut(s) 268, 929, 2223, 2251
BciT130I CCWGG 6 cut(s) 1434, 1886, 2109, 2211, 2391, 2487
BciVI GTATCC 2 cut(s) 1885, 2357
BclI TGATCA 1 cut(s) 1621
BcoDI GTCTC 5 cut(s) 233, 341, 462, 1456, 1606
BfaI CTAG 4 cut(s) 362, 1007, 2042, 2129
BfmI CTRYAG 2 cut(s) 441, 1477
BfrI CTTAAG 1 cut(s) 1204
BfuI GTATCC 2 cut(s) 1885, 2357
BglI GCCNNNNNGGC 1 cut(s) 1074
BglII AGATCT 2 cut(s) 956, 2038
BisI GCNGC 4 cut(s) 119, 444, 675, 1996
BlnI CCTAGG 1 cut(s) 1006
BlsI GCNGC 4 cut(s) 120, 445, 676, 1997
BmcAI AGTACT 3 cut(s) 135, 1319, 2259
Bme1390I CCNGG 6 cut(s) 1434, 1886, 2109, 2211, 2391, 2487
Bme18I GGWCC 3 cut(s) 973, 2387, 2483
BmeT110I CYCGRG 2 cut(s) 76, 375
BmgT120I GGNCC 7 cut(s) 435, 436, 454, 775, 973, 2387, 2483
BmrFI CCNGG 6 cut(s) 1434, 1886, 2109, 2211, 2391, 2487
BmrI ACTGGG 2 cut(s) 706, 1183
BmsI GCATC 5 cut(s) 816, 1333, 1350, 1419, 2128
BmuI ACTGGG 2 cut(s) 706, 1183
BpiI GAAGAC 3 cut(s) 54, 414, 2016
BplI GAGNNNNNCTC 4 cut(s) 76, 108, 2303, 2335
BpmI CTGGAG 1 cut(s) 1238
Bpu10I CCTNAGC 1 cut(s) 447
Bpu14I TTCGAA 1 cut(s) 2145
BsaBI GATNNNNATC 2 cut(s) 555, 1896
BsaHI GRCGYC 1 cut(s) 1873
BsaJI CCNNGG 8 cut(s) 66, 83, 753, 1006, 1279, 2108, 2210, 2280
BsaWI WCCGGW 1 cut(s) 591
BsaXI ACNNNNNCTCC 8 cut(s) 597, 603, 627, 633, 1214, 1244, 2028, 2058
Bsc4I CCNNNNNNNGG 6 cut(s) 433, 686, 883, 1206, 1439, 1841
Bse1I ACTGG 3 cut(s) 701, 1178, 2066
Bse21I CCTNAGG 1 cut(s) 969
Bse3DI GCAATG 2 cut(s) 1494, 1852
Bse8I GATNNNNATC 2 cut(s) 555, 1896
BseBI CCWGG 6 cut(s) 1434, 1886, 2109, 2211, 2391, 2487
BseDI CCNNGG 8 cut(s) 66, 83, 753, 1006, 1279, 2108, 2210, 2280
BseGI GGATG 8 cut(s) 94, 485, 1033, 1434, 1494, 2062, 2276, 2305
BseJI GATNNNNATC 2 cut(s) 555, 1896
BseLI CCNNNNNNNGG 6 cut(s) 433, 686, 883, 1206, 1439, 1841
BseMI GCAATG 2 cut(s) 1494, 1852
BseMII CTCAG 4 cut(s) 461, 810, 960, 1602
BseNI ACTGG 3 cut(s) 701, 1178, 2066
BseRI GAGGAG 2 cut(s) 1235, 2327
BseSI GKGCMC 1 cut(s) 439
BseX3I CGGCCG 1 cut(s) 2236
BseXI GCAGC 4 cut(s) 105, 455, 686, 2007
BseYI CCCAGC 1 cut(s) 4
BsgI GTGCAG 1 cut(s) 1888
Bsh1236I CGCG 2 cut(s) 1725, 2272
Bsh1285I CGRYCG 1 cut(s) 2239
BshFI GGCC 7 cut(s) 437, 455, 776, 1068, 1447, 1493, 2238
BsiEI CGRYCG 1 cut(s) 2239
BsiHKCI CYCGRG 2 cut(s) 76, 375
BsiSI CCGG 1 cut(s) 592
BslFI GGGAC 2 cut(s) 124, 2226
BslI CCNNNNNNNGG 6 cut(s) 433, 686, 883, 1206, 1439, 1841
BsmAI GTCTC 5 cut(s) 233, 341, 462, 1456, 1606
BsmBI CGTCTC 1 cut(s) 233
BsmFI GGGAC 2 cut(s) 124, 2226
BsmI GAATGC 2 cut(s) 898, 1652
BsnI GGCC 7 cut(s) 437, 455, 776, 1068, 1447, 1493, 2238
BsoBI CYCGRG 2 cut(s) 76, 375
Bsp119I TTCGAA 1 cut(s) 2145
Bsp120I GGGCCC 1 cut(s) 435
Bsp1286I GDGCHC 3 cut(s) 11, 439, 491
Bsp19I CCATGG 1 cut(s) 1279
BspACI CCGC 6 cut(s) 116, 805, 1015, 1186, 1725, 2270
BspANI GGCC 7 cut(s) 437, 455, 776, 1068, 1447, 1493, 2238
BspCNI CTCAG 4 cut(s) 460, 809, 961, 1603
BspFNI CGCG 2 cut(s) 1725, 2272
BspHI TCATGA 1 cut(s) 1618
BspMAI CTGCAG 1 cut(s) 445
BspPI GGATC 7 cut(s) 653, 666, 695, 708, 1372, 2454, 2467
BspT104I TTCGAA 1 cut(s) 2145
BspTI CTTAAG 1 cut(s) 1204
BsrDI GCAATG 2 cut(s) 1494, 1852
BsrI ACTGG 3 cut(s) 701, 1178, 2066
BssECI CCNNGG 8 cut(s) 66, 83, 753, 1006, 1279, 2108, 2210, 2280
BssNI GRCGYC 1 cut(s) 1873
BssT1I CCWWGG 4 cut(s) 66, 753, 1006, 1279
Bst2UI CCWGG 6 cut(s) 1434, 1886, 2109, 2211, 2391, 2487
Bst4CI ACNGT 9 cut(s) 138, 226, 428, 655, 874, 1040, 1405, 1717, 2135
Bst6I CTCTTC 3 cut(s) 1163, 1533, 2158
BstACI GRCGYC 1 cut(s) 1873
BstAFI CTTAAG 1 cut(s) 1204
BstAPI GCANNNNNTGC 1 cut(s) 671
BstBI TTCGAA 1 cut(s) 2145
BstC8I GCNNGC 2 cut(s) 1365, 1485
BstDEI CTNAG 4 cut(s) 447, 796, 969, 1611
BstDSI CCRYGG 2 cut(s) 1279, 2280
BstF5I GGATG 8 cut(s) 94, 485, 1033, 1434, 1494, 2062, 2276, 2305
BstFNI CGCG 2 cut(s) 1725, 2272
BstMAI GTCTC 5 cut(s) 233, 341, 462, 1456, 1606
BstMCI CGRYCG 1 cut(s) 2239
BstMWI GCNNNNNNNGC 4 cut(s) 443, 452, 671, 1074
BstNI CCWGG 6 cut(s) 1434, 1886, 2109, 2211, 2391, 2487
BstSCI CCNGG 6 cut(s) 1432, 1884, 2107, 2209, 2389, 2485
BstSFI CTRYAG 2 cut(s) 441, 1477
BstSLI GKGCMC 1 cut(s) 439
BstUI CGCG 2 cut(s) 1725, 2272
BstV1I GCAGC 4 cut(s) 105, 455, 686, 2007
BstV2I GAAGAC 3 cut(s) 54, 414, 2016
BstX2I RGATCY 5 cut(s) 658, 700, 956, 2038, 2459
BstXI CCANNNNNNTGG 1 cut(s) 464
BstYI RGATCY 5 cut(s) 658, 700, 956, 2038, 2459
BstZI CGGCCG 1 cut(s) 2236
Bsu36I CCTNAGG 1 cut(s) 969
BsuI GTATCC 2 cut(s) 1885, 2357
BsuRI GGCC 7 cut(s) 437, 455, 776, 1068, 1447, 1493, 2238
BtgI CCRYGG 2 cut(s) 1279, 2280
BtsCI GGATG 8 cut(s) 94, 485, 1033, 1434, 1494, 2062, 2276, 2305
BtsIMutI CAGTG 5 cut(s) 651, 1059, 1330, 1554, 2140
Cac8I GCNNGC 2 cut(s) 1365, 1485
CciI TCATGA 1 cut(s) 1618
Cfr13I GGNCC 7 cut(s) 435, 436, 454, 775, 973, 2387, 2483
CseI GACGC 1 cut(s) 1881
CsiI ACCWGGT 1 cut(s) 1884
Csp6I GTAC 9 cut(s) 134, 286, 424, 694, 1318, 1678, 2086, 2116, 2258
CviQI GTAC 9 cut(s) 134, 286, 424, 694, 1318, 1678, 2086, 2116, 2258
DdeI CTNAG 4 cut(s) 447, 796, 969, 1611
EaeI YGGCCR 2 cut(s) 1491, 2236
EagI CGGCCG 1 cut(s) 2236
Eam1104I CTCTTC 3 cut(s) 1163, 1533, 2158
EarI CTCTTC 3 cut(s) 1163, 1533, 2158
EclXI CGGCCG 1 cut(s) 2236
Eco130I CCWWGG 4 cut(s) 66, 753, 1006, 1279
Eco24I GRGCYC 3 cut(s) 11, 439, 491
Eco47I GGWCC 3 cut(s) 973, 2387, 2483
Eco52I CGGCCG 1 cut(s) 2236
Eco57I CTGAAG 2 cut(s) 1306, 1476
Eco81I CCTNAGG 1 cut(s) 969
Eco88I CYCGRG 2 cut(s) 76, 375
EcoO109I RGGNCCY 2 cut(s) 436, 973
EcoRI GAATTC 1 cut(s) 368
EcoRII CCWGG 6 cut(s) 1432, 1884, 2107, 2209, 2389, 2485
EcoT14I CCWWGG 4 cut(s) 66, 753, 1006, 1279
EcoT38I GRGCYC 3 cut(s) 11, 439, 491
ErhI CCWWGG 4 cut(s) 66, 753, 1006, 1279
Esp3I CGTCTC 1 cut(s) 233
FaqI GGGAC 2 cut(s) 124, 2226
FauI CCCGC 3 cut(s) 812, 1008, 1718
FauNDI CATATG 1 cut(s) 682
FbaI TGATCA 1 cut(s) 1621
FblI GTMKAC 1 cut(s) 2181
Fnu4HI GCNGC 4 cut(s) 119, 444, 675, 1996
FokI GGATG 8 cut(s) 81, 492, 1040, 1441, 1481, 2049, 2263, 2312
FriOI GRGCYC 3 cut(s) 11, 439, 491
Fsp4HI GCNGC 4 cut(s) 119, 444, 675, 1996
FspBI CTAG 4 cut(s) 362, 1007, 2042, 2129
GluI GCNGC 4 cut(s) 119, 444, 675, 1996
GsaI CCCAGC 1 cut(s) 8
GsuI CTGGAG 1 cut(s) 1238
HaeIII GGCC 7 cut(s) 437, 455, 776, 1068, 1447, 1493, 2238
HapII CCGG 1 cut(s) 592
HgaI GACGC 1 cut(s) 1881
Hin1I GRCGYC 1 cut(s) 1873
HindIII AAGCTT 1 cut(s) 1704
HinfI GANTC 5 cut(s) 79, 269, 1103, 1155, 1615
HpaII CCGG 1 cut(s) 592
HphI GGTGA 4 cut(s) 238, 1268, 1649, 2256
Hpy166II GTNNAC 5 cut(s) 424, 694, 2071, 2156, 2182
Hpy188I TCNGA 7 cut(s) 84, 715, 799, 1377, 1456, 2410, 2506
Hpy188III TCNNGA 7 cut(s) 362, 449, 535, 960, 1619, 2198, 2219
Hpy8I GTNNAC 5 cut(s) 424, 694, 2071, 2156, 2182
HpyAV CCTTC 8 cut(s) 227, 347, 502, 925, 1451, 1693, 2195, 2260
HpyCH4III ACNGT 9 cut(s) 138, 226, 428, 655, 874, 1040, 1405, 1717, 2135
HpyCH4IV ACGT 1 cut(s) 1290
HpyF10VI GCNNNNNNNGC 4 cut(s) 443, 452, 671, 1074
HpyF3I CTNAG 4 cut(s) 447, 796, 969, 1611
HpySE526I ACGT 1 cut(s) 1290
Hsp92I GRCGYC 1 cut(s) 1873
Ksp22I TGATCA 1 cut(s) 1621
LmnI GCTCC 8 cut(s) 172, 280, 325, 494, 682, 763, 895, 1566
Lsp1109I GCAGC 4 cut(s) 105, 455, 686, 2007
LweI GCATC 5 cut(s) 816, 1333, 1350, 1419, 2128
MabI ACCWGGT 1 cut(s) 1884
MaeI CTAG 4 cut(s) 362, 1007, 2042, 2129
MaeII ACGT 1 cut(s) 1290
MaeIII GTNAC 6 cut(s) 928, 1057, 1255, 1549, 1637, 2099
MfeI CAATTG 1 cut(s) 1629
MflI RGATCY 5 cut(s) 658, 700, 956, 2038, 2459
MhlI GDGCHC 3 cut(s) 11, 439, 491
MlsI TGGCCA 1 cut(s) 1493
MluNI TGGCCA 1 cut(s) 1493
MlyI GAGTC 5 cut(s) 88, 263, 1112, 1149, 1624
MmeI TCCRAC 7 cut(s) 150, 170, 445, 763, 1224, 2341, 2437
Mox20I TGGCCA 1 cut(s) 1493
MscI TGGCCA 1 cut(s) 1493
MslI CAYNNNNRTG 6 cut(s) 606, 1041, 1278, 1289, 2379, 2475
Msp20I TGGCCA 1 cut(s) 1493
MspA1I CMGCKG 2 cut(s) 118, 880
MspCI CTTAAG 1 cut(s) 1204
MspI CCGG 1 cut(s) 592
MspR9I CCNGG 6 cut(s) 1434, 1886, 2109, 2211, 2391, 2487
MunI CAATTG 1 cut(s) 1629
Mva1269I GAATGC 2 cut(s) 898, 1652
MvaI CCWGG 6 cut(s) 1434, 1886, 2109, 2211, 2391, 2487
MvnI CGCG 2 cut(s) 1725, 2272
MwoI GCNNNNNNNGC 4 cut(s) 443, 452, 671, 1074
NcoI CCATGG 1 cut(s) 1279
NdeI CATATG 1 cut(s) 682
NmuCI GTSAC 5 cut(s) 928, 1057, 1549, 1637, 2099
NspV TTCGAA 1 cut(s) 2145
PaeR7I CTCGAG 1 cut(s) 375
PagI TCATGA 1 cut(s) 1618
PctI GAATGC 2 cut(s) 898, 1652
PflFI GACNNNGTC 1 cut(s) 1440
PflMI CCANNNNNTGG 3 cut(s) 686, 883, 1841
PfoI TCCNGGA 2 cut(s) 2389, 2485
PkrI GCNGC 4 cut(s) 120, 445, 676, 1997
PleI GAGTC 5 cut(s) 87, 263, 1111, 1149, 1623
PpsI GAGTC 5 cut(s) 87, 263, 1111, 1149, 1623
PpuMI RGGWCCY 1 cut(s) 973
PsiI TTATAA 2 cut(s) 1311, 1757
Psp5II RGGWCCY 1 cut(s) 973
Psp6I CCWGG 6 cut(s) 1432, 1884, 2107, 2209, 2389, 2485
PspFI CCCAGC 1 cut(s) 4
PspGI CCWGG 6 cut(s) 1432, 1884, 2107, 2209, 2389, 2485
PspOMI GGGCCC 1 cut(s) 435
PspPI GGNCC 7 cut(s) 435, 436, 454, 775, 973, 2387, 2483
PspPPI RGGWCCY 1 cut(s) 973
PspXI VCTCGAGB 1 cut(s) 375
PstI CTGCAG 1 cut(s) 445
PsuI RGATCY 5 cut(s) 658, 700, 956, 2038, 2459
PsyI GACNNNGTC 1 cut(s) 1440
PvuII CAGCTG 1 cut(s) 880
RsaI GTAC 9 cut(s) 135, 287, 425, 695, 1319, 1679, 2087, 2117, 2259
RsaNI GTAC 9 cut(s) 134, 286, 424, 694, 1318, 1678, 2086, 2116, 2258
RseI CAYNNNNRTG 6 cut(s) 606, 1041, 1278, 1289, 2379, 2475
SatI GCNGC 4 cut(s) 119, 444, 675, 1996
Sau96I GGNCC 7 cut(s) 435, 436, 454, 775, 973, 2387, 2483
ScaI AGTACT 3 cut(s) 135, 1319, 2259
SchI GAGTC 5 cut(s) 88, 263, 1112, 1149, 1624
ScrFI CCNGG 6 cut(s) 1434, 1886, 2109, 2211, 2391, 2487
SduI GDGCHC 3 cut(s) 11, 439, 491
SexAI ACCWGGT 1 cut(s) 1884
SfaNI GCATC 5 cut(s) 816, 1333, 1350, 1419, 2128
SfcI CTRYAG 2 cut(s) 441, 1477
Sfr274I CTCGAG 1 cut(s) 375
SfuI TTCGAA 1 cut(s) 2145
SinI GGWCC 3 cut(s) 973, 2387, 2483
SlaI CTCGAG 1 cut(s) 375
SmiMI CAYNNNNRTG 6 cut(s) 606, 1041, 1278, 1289, 2379, 2475
SmlI CTYRAG 2 cut(s) 375, 1204
SmoI CTYRAG 2 cut(s) 375, 1204
SsiI CCGC 6 cut(s) 116, 805, 1015, 1186, 1725, 2270
SspMI CTAG 4 cut(s) 362, 1007, 2042, 2129
StyD4I CCNGG 6 cut(s) 1432, 1884, 2107, 2209, 2389, 2485
StyI CCWWGG 4 cut(s) 66, 753, 1006, 1279
TaaI ACNGT 9 cut(s) 138, 226, 428, 655, 874, 1040, 1405, 1717, 2135
TaiI ACGT 1 cut(s) 1293
TaqI TCGA 6 cut(s) 124, 215, 376, 853, 1248, 2145
TatI WGTACW 6 cut(s) 133, 693, 1317, 1677, 2115, 2257
TscAI CASTG 5 cut(s) 658, 1066, 1330, 1554, 2140
TseFI GTSAC 5 cut(s) 928, 1057, 1549, 1637, 2099
TseI GCWGC 4 cut(s) 118, 443, 674, 1995
Tsp45I GTSAC 5 cut(s) 928, 1057, 1549, 1637, 2099
TspGWI ACGGA 2 cut(s) 257, 2269
TspRI CASTG 5 cut(s) 658, 1066, 1330, 1554, 2140
Tth111I GACNNNGTC 1 cut(s) 1440
Van91I CCANNNNNTGG 3 cut(s) 686, 883, 1841
Vha464I CTTAAG 1 cut(s) 1204
VpaK11BI GGWCC 3 cut(s) 973, 2387, 2483
XbaI TCTAGA 1 cut(s) 361
XcmI CCANNNNNNNNNTGG 1 cut(s) 499
XhoI CTCGAG 1 cut(s) 375
XmaJI CCTAGG 1 cut(s) 1006
XmiI GTMKAC 1 cut(s) 2181
XspI CTAG 4 cut(s) 362, 1007, 2042, 2129
ZrmI AGTACT 3 cut(s) 135, 1319, 2259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.