Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6D

Orthogroup ID

OG0013627

Physical Location & Seq

Genomic Coordinates Reverse (-)

61808789 .. 61813828

Gene Structure 5040 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6DG442800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2436 bp

ATGTTGGGTTCTTTGGGTCTTCATCTCACTTGTTCTTTTGAGTCTTTATCGTGTTCCAAGGACTACCTGAGTCCGAGGGTAACTCAAACTAAAGCAGTCTCTTTTCAGCTTCGACATAGAGTATTGTCCCCTCCAGGGGGTTTTAGATGTGGGAAGTTGGAGCGTAGCAGTCCCAGAGTTTCAGCAGTTTTGACAGACGACAGCTCGAAAATGACAGTCAGTGATGAAGAGATGGAAGATATTGGTGTTGTGAGAACAGATTCAAACTTGGAGCCATACAAAGATCACTTCAAGCATCGAATAACAAGATATTTGGAGCAGAAGAGTCTCTTTGAGAAATATGAAGGAGGTTTAGAGGAATTCGCGCAAGGTTATGTAAAATTTGGATTTAACAGAGAAGATGGTGGAATTATGTACCGTGAATGGGCCCCTGCAGCTCAGGAGGCACAACTTGTTGGAGACTTTAATGGATGGGATGGCGGCAAATACGAGATGGAGAAGGGCGAATTTGGGGTATGGAGTATTAAGATACCCGATTTTGATGGAAGTCCAGCTATTCCTCACAATTCAAAGGTTAAATTCCGGTTCAAGCATGGAAATGGAGTTTGGGTTGATCGTATTCCTGCTTGGATTAAATATGCCACTGTGGACCCAACAAGATTTGCAGCTCCATATGATGGTGTGTACTGGGATCCACCACCATCAGAAAGGTATCAGTTTAAGTACGGTCGTCCTCCAAAACCCAAGGCTCCACGAATATATGAGGCCCATGTTGGAATGAGTAGCTCAGAACCCCGCATCAGTTCATACAGAGAATTTGCTGATAATGTTTTGCCTCGTATCCAAGCAAATAACTATAACACAGTCCAGCTGATGGCCGTGATGGAGCATTCCTATTATGCATCCTTCGGTTATCATGTGACAAACTTCTTTGCCGTAAGCAGTAGATCTGGAACACCTGAGGACCTTAAATATCTAATTGATAAAGCACATTCCCTAGGTTTGCGTGTATTGATGGATGTTGTTCACAGTCATGCAAGTAATAACGTCACTGATGGCCTCAGTGGCTTTGAAGTTGGTCAAAGCACACAAGATTCCTATTTCCACACAGGAGATAGAGGCTACCATAAGCTATGGGATAGCCGACTTTTCAACTATGCTAATTGGGAAGTTCTCCGCTTCTTTTTATCCAACTTAAGGTGGTGGCTGGAGGAGTATCAATTTGATGGGTTTCGATTTGATGGAGTAACTTCCATGCTGTATCATCACCATGGCATAAATATGGCATTTACAGGGAATTATGATGAGTACTTCAGTGAGGCAACAGATGTTGATGCTGTTGTTTATTTGATGCTTGCCAACTATCTGATCCATAAAATATTGCCATCTGCAACTGTAATTGCTGAAGATGTTTCTGGGATGCCTGGACTTGGTCGGCCTGTCTCTGAAGGAGGTGTTGGTTTTGACTACCGGCTGGCAATGGCCATCCCTGACAAATGGATTGATTATGTGAAAAACAAGAAAGACGAAGAGTGGTCGATGAAGGAAATTTCATGGAGCTTGACGAATAGGAGATACACTGAGAAGTGTGTTTCCTATGCTGAGAGTCATGACCAGGCAATTGTGGGTGACAAGACGATTGCATTCTTTTTGATGGATAAAGAAATGTACTCTGGCATGTCTTGTTTGAATGAAGCATCTCCTACTGTTGAGCGCGGGGTTGCTCTTCACAAGATGATACATTTTATAACTATGGCATTAGGAGGCGAGGGTTACCTTAACTTTATGGGTAATGAGTTTGGACACCCTGAGTGGATCGACTTTCCAAGAGAAGGCAATGAGTGGAGTTATGAAAAGTGCAGACGCCAATGGAACCTGGTGGATACAGATCATTTAAGATATAAGTTTATGAACGCATTTGACAGAGCTATGCACTTGCTTGATGAGAAATTTTCGTTCCTTTCATCAACGAAGCAGATAGTGAGCAGCACAAATGAAGAAGACAAGGTTATTGTCTTTGAGCGTGGAGATCTAGTTTTTGTATTTAATTTTCATCCAGTAAACACATACGATGGGTACAAAGTGGGGTGTGACTTACCTGGGAAGTACAGAGTTGCACTAGATAGTGATGCTTTTGAATTCGGTGGACCTGGAAGAGTAGGCCATGATGTAGACCACTTCACATTCCCAGAAGGAATACCAGGGGTTCCTGAAACAAATTTCAACGGCCGTCCCAACTCCTTAAGAGTACTCTCACCACCGCGAACATGCGTGGTTTATTATCGAGTGGATGAAAGTAAGGAGACTGCTGTGAAGGAGGACTCGGCAGCAGAAGTTGTGGTTGGGCGGGAGAACTCCCAATTGCTTGGATCCGTGAAAGATGGAAATGTTGGTCCAGGAGTGGAAGAGACCGTCAAAGTTTCGTCAGATAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003844 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0006073 GO:0008150 GO:0008152 GO:0009501 GO:0009507 GO:0009536 GO:0009987 GO:0016740 GO:0016757 GO:0016758 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase Alpha-amylase_C CBM_48

Protein Analysis

811

Amino Acids

92.32

Weight (kDa)

5.58

Isoelectric Point (pI)

33.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_48	PF02922	127 - 210	1.1e-19	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Alpha-amylase	PF00128	300 - 352	3.3e-09	Alpha amylase, catalytic domain
Alpha-amylase_C	PF02806	662 - 762	5e-20	Alpha amylase, C-terminal all-beta domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013627)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g37381 FvH4_2g37410
malus_domestica	MD08G1002300.v1.1
prunus_persica	Prupe.1G354000_v2.0.a1 Prupe.1G354000_v2.0.a1 Prupe.1G354000_v2.0.a1
pyrus_communis	pycom08g00150
rosa_chinensis	RchiOBHm_Chr6g0304311
rosa_laevigata	RLG00000010992
rosa_multiflora	Rmu_ssc0000218.1_g000037
rosa_roxburghii	Rroxscaffold_7G00163850
rosa_rugosa	Rorug06G0329900 Rorug06G0330000
rosa_samantha	Rh6AG443300 Rh6BG459100 Rh6CG456100 Rh6DG442800
rosa_wichuraiana	Rw6G038470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1748
AccB7I	CCANNNNNTGG	2 cut(s)	677, 874
AccI	GTMKAC	1 cut(s)	2172
AccII	CGCG	3 cut(s)	365, 1716, 2263
AciI	CCGC	6 cut(s)	480, 796, 1177, 1716, 2261, 2347
AclWI	GGATC	6 cut(s)	686, 699, 1363, 1823, 2364, 2377
AcoI	YGGCCR	3 cut(s)	876, 1482, 2227
AcsI	RAATTY	9 cut(s)	359, 380, 506, 578, 815, 1548, 1949, 2138, 2218
AcuI	CTGAAG	3 cut(s)	1297, 1425, 1467
AcyI	GRCGYC	1 cut(s)	1864
AfaI	GTAC	8 cut(s)	416, 686, 725, 1310, 1670, 2078, 2108, 2250
AfiI	CCNNNNNNNGG	9 cut(s)	135, 136, 137, 424, 677, 874, 1197, 1430, 1832
AflII	CTTAAG	2 cut(s)	1195, 2242
AgsI	TTSAA	9 cut(s)	264, 292, 570, 589, 1073, 1153, 1690, 2138, 2224
AjnI	CCWGG	8 cut(s)	133, 1423, 1614, 1875, 2098, 2149, 2200, 2395
AjuI	GAANNNNNNNTTGG	6 cut(s)	589, 621, 1440, 1472, 2325, 2357
Alw26I	GTCTC	6 cut(s)	103, 332, 453, 1447, 2297, 2402
AlwI	GGATC	6 cut(s)	686, 699, 1363, 1823, 2364, 2377
AoxI	GGCC	8 cut(s)	426, 765, 876, 1057, 1436, 1482, 2161, 2227
ApaI	GGGCCC	1 cut(s)	430
ApeKI	GCWGC	4 cut(s)	434, 665, 1986, 2327
ApoI	RAATTY	9 cut(s)	359, 380, 506, 578, 815, 1548, 1949, 2138, 2218
Asp700I	GAANNNNTTC	1 cut(s)	259
AspA2I	CCTAGG	1 cut(s)	997
AspLEI	GCGC	2 cut(s)	367, 1716
AspS9I	GGNCC	7 cut(s)	426, 427, 649, 766, 964, 2147, 2393
AsuHPI	GGTGA	3 cut(s)	1259, 1640, 2247
AvaII	GGWCC	4 cut(s)	649, 964, 2147, 2393
AvrII	CCTAGG	1 cut(s)	997
AxyI	CCTNAGG	1 cut(s)	960
BaeGI	GKGCMC	1 cut(s)	430
BalI	TGGCCA	1 cut(s)	1484
BamHI	GGATCC	2 cut(s)	691, 2369
BanII	GRGCYC	1 cut(s)	430
BbsI	GAAGAC	2 cut(s)	11, 2007
BbvI	GCAGC	4 cut(s)	446, 677, 1998, 2339
BceAI	ACGGC	4 cut(s)	863, 920, 2214, 2242
BciT130I	CCWGG	8 cut(s)	135, 1425, 1616, 1877, 2100, 2151, 2202, 2397
BciVI	GTATCC	2 cut(s)	851, 1876
BcoDI	GTCTC	6 cut(s)	103, 332, 453, 1447, 2297, 2402
BfaI	CTAG	3 cut(s)	998, 2033, 2120
BfmI	CTRYAG	1 cut(s)	432
BfrI	CTTAAG	2 cut(s)	1195, 2242
BfuI	GTATCC	2 cut(s)	851, 1876
BglI	GCCNNNNNGGC	1 cut(s)	1065
BglII	AGATCT	2 cut(s)	947, 2029
BisI	GCNGC	5 cut(s)	435, 481, 666, 1987, 2328
BlnI	CCTAGG	1 cut(s)	997
BlsI	GCNGC	5 cut(s)	436, 482, 667, 1988, 2329
BmcAI	AGTACT	2 cut(s)	1310, 2250
Bme1390I	CCNGG	8 cut(s)	135, 1425, 1616, 1877, 2100, 2151, 2202, 2397
Bme18I	GGWCC	4 cut(s)	649, 964, 2147, 2393
BmgT120I	GGNCC	7 cut(s)	426, 427, 649, 766, 964, 2147, 2393
BmiI	GGNNCC	9 cut(s)	273, 428, 429, 651, 693, 750, 1874, 2208, 2371
BmrFI	CCNGG	8 cut(s)	135, 1425, 1616, 1877, 2100, 2151, 2202, 2397
BmrI	ACTGGG	1 cut(s)	697
BmsI	GCATC	8 cut(s)	304, 807, 911, 1324, 1341, 1410, 1706, 2119
BmuI	ACTGGG	1 cut(s)	697
BpiI	GAAGAC	2 cut(s)	11, 2007
BplI	GAGNNNNNCTC	2 cut(s)	67, 99
BpmI	CTGGAG	2 cut(s)	117, 1229
Bpu10I	CCTNAGC	1 cut(s)	438
BsaBI	GATNNNNATC	1 cut(s)	1887
BsaHI	GRCGYC	1 cut(s)	1864
BsaI	GGTCTC	1 cut(s)	2402
BsaJI	CCNNGG	8 cut(s)	57, 74, 134, 744, 997, 1270, 2099, 2201
BsaWI	WCCGGW	1 cut(s)	582
BsaXI	ACNNNNNCTCC	4 cut(s)	594, 624, 2019, 2049
Bsc4I	CCNNNNNNNGG	9 cut(s)	135, 136, 137, 424, 677, 874, 1197, 1430, 1832
Bse118I	RCCGGY	1 cut(s)	1470
Bse1I	ACTGG	2 cut(s)	692, 2057
Bse21I	CCTNAGG	1 cut(s)	960
Bse3DI	GCAATG	2 cut(s)	1485, 1843
Bse8I	GATNNNNATC	1 cut(s)	1887
BseBI	CCWGG	8 cut(s)	135, 1425, 1616, 1877, 2100, 2151, 2202, 2397
BseDI	CCNNGG	8 cut(s)	57, 74, 134, 744, 997, 1270, 2099, 2201
BseGI	GGATG	8 cut(s)	476, 481, 902, 1024, 1425, 1485, 2053, 2296
BseJI	GATNNNNATC	1 cut(s)	1887
BseLI	CCNNNNNNNGG	9 cut(s)	135, 136, 137, 424, 677, 874, 1197, 1430, 1832
BseMI	GCAATG	2 cut(s)	1485, 1843
BseMII	CTCAG	8 cut(s)	59, 452, 801, 951, 1075, 1572, 1593, 1800
BseNI	ACTGG	2 cut(s)	692, 2057
BseRI	GAGGAG	1 cut(s)	1226
BseSI	GKGCMC	1 cut(s)	430
BseX3I	CGGCCG	1 cut(s)	2227
BseXI	GCAGC	4 cut(s)	446, 677, 1998, 2339
BsgI	GTGCAG	1 cut(s)	1879
Bsh1236I	CGCG	3 cut(s)	365, 1716, 2263
Bsh1285I	CGRYCG	2 cut(s)	730, 2230
BshFI	GGCC	8 cut(s)	428, 767, 878, 1059, 1438, 1484, 2163, 2229
BsiEI	CGRYCG	2 cut(s)	730, 2230
BsiSI	CCGG	2 cut(s)	583, 1471
BslFI	GGGAC	3 cut(s)	112, 156, 2217
BslI	CCNNNNNNNGG	9 cut(s)	135, 136, 137, 424, 677, 874, 1197, 1430, 1832
BsmAI	GTCTC	6 cut(s)	103, 332, 453, 1447, 2297, 2402
BsmFI	GGGAC	3 cut(s)	112, 156, 2217
BsmI	GAATGC	2 cut(s)	889, 1643
BsnI	GGCC	8 cut(s)	428, 767, 878, 1059, 1438, 1484, 2163, 2229
Bso31I	GGTCTC	1 cut(s)	2402
Bsp120I	GGGCCC	1 cut(s)	426
Bsp1286I	GDGCHC	1 cut(s)	430
Bsp143I	GATC	9 cut(s)	283, 613, 691, 947, 1368, 1815, 1888, 2029, 2369
Bsp19I	CCATGG	1 cut(s)	1270
BspACI	CCGC	6 cut(s)	480, 796, 1177, 1716, 2261, 2347
BspANI	GGCC	8 cut(s)	428, 767, 878, 1059, 1438, 1484, 2163, 2229
BspCNI	CTCAG	8 cut(s)	60, 451, 800, 952, 1074, 1573, 1594, 1801
BspFNI	CGCG	3 cut(s)	365, 1716, 2263
BspHI	TCATGA	1 cut(s)	1609
BspLI	GGNNCC	9 cut(s)	273, 428, 429, 651, 693, 750, 1874, 2208, 2371
BspMAI	CTGCAG	1 cut(s)	436
BspPI	GGATC	6 cut(s)	686, 699, 1363, 1823, 2364, 2377
BspQI	GCTCTTC	1 cut(s)	1731
BspTI	CTTAAG	2 cut(s)	1195, 2242
BspTNI	GGTCTC	1 cut(s)	2402
BsrDI	GCAATG	2 cut(s)	1485, 1843
BsrFI	RCCGGY	1 cut(s)	1470
BsrI	ACTGG	2 cut(s)	692, 2057
BssAI	RCCGGY	1 cut(s)	1470
BssECI	CCNNGG	8 cut(s)	57, 74, 134, 744, 997, 1270, 2099, 2201
BssMI	GATC	9 cut(s)	283, 613, 691, 947, 1368, 1815, 1888, 2029, 2369
BssNI	GRCGYC	1 cut(s)	1864
BssT1I	CCWWGG	4 cut(s)	57, 744, 997, 1270
Bst2UI	CCWGG	8 cut(s)	135, 1425, 1616, 1877, 2100, 2151, 2202, 2397
Bst4CI	ACNGT	9 cut(s)	217, 419, 646, 728, 865, 1031, 1396, 1708, 2413
Bst6I	CTCTTC	6 cut(s)	222, 317, 1524, 1731, 2149, 2400
BstACI	GRCGYC	1 cut(s)	1864
BstAFI	CTTAAG	2 cut(s)	1195, 2242
BstC8I	GCNNGC	2 cut(s)	1356, 1476
BstDEI	CTNAG	8 cut(s)	68, 438, 787, 960, 1061, 1581, 1602, 1809
BstDSI	CCRYGG	1 cut(s)	1270
BstEII	GGTNACC	1 cut(s)	1772
BstF5I	GGATG	8 cut(s)	476, 481, 902, 1024, 1425, 1485, 2053, 2296
BstFNI	CGCG	3 cut(s)	365, 1716, 2263
BstHHI	GCGC	2 cut(s)	367, 1716
BstKTI	GATC	9 cut(s)	286, 616, 694, 950, 1371, 1818, 1891, 2032, 2372
BstMAI	GTCTC	6 cut(s)	103, 332, 453, 1447, 2297, 2402
BstMBI	GATC	9 cut(s)	283, 613, 691, 947, 1368, 1815, 1888, 2029, 2369
BstMCI	CGRYCG	2 cut(s)	730, 2230
BstMWI	GCNNNNNNNGC	3 cut(s)	434, 443, 1065
BstNI	CCWGG	8 cut(s)	135, 1425, 1616, 1877, 2100, 2151, 2202, 2397
BstNSI	RCATGY	2 cut(s)	1681, 2271
BstPI	GGTNACC	1 cut(s)	1772
BstSCI	CCNGG	8 cut(s)	133, 1423, 1614, 1875, 2098, 2149, 2200, 2395
BstSFI	CTRYAG	1 cut(s)	432
BstSLI	GKGCMC	1 cut(s)	430
BstUI	CGCG	3 cut(s)	365, 1716, 2263
BstV1I	GCAGC	4 cut(s)	446, 677, 1998, 2339
BstV2I	GAAGAC	2 cut(s)	11, 2007
BstX2I	RGATCY	4 cut(s)	691, 947, 2029, 2369
BstXI	CCANNNNNNTGG	1 cut(s)	2366
BstYI	RGATCY	4 cut(s)	691, 947, 2029, 2369
BstZI	CGGCCG	1 cut(s)	2227
Bsu36I	CCTNAGG	1 cut(s)	960
BsuI	GTATCC	2 cut(s)	851, 1876
BsuRI	GGCC	8 cut(s)	428, 767, 878, 1059, 1438, 1484, 2163, 2229
BtgI	CCRYGG	1 cut(s)	1270
BtsCI	GGATG	8 cut(s)	476, 481, 902, 1024, 1425, 1485, 2053, 2296
BtsIMutI	CAGTG	6 cut(s)	226, 642, 1050, 1069, 1321, 1578
Cac8I	GCNNGC	2 cut(s)	1356, 1476
CciI	TCATGA	1 cut(s)	1609
CfoI	GCGC	2 cut(s)	367, 1716
Cfr10I	RCCGGY	1 cut(s)	1470
Cfr13I	GGNCC	7 cut(s)	426, 427, 649, 766, 964, 2147, 2393
CseI	GACGC	1 cut(s)	1872
CsiI	ACCWGGT	1 cut(s)	1875
Csp6I	GTAC	8 cut(s)	415, 685, 724, 1309, 1669, 2077, 2107, 2249
CviQI	GTAC	8 cut(s)	415, 685, 724, 1309, 1669, 2077, 2107, 2249
DdeI	CTNAG	8 cut(s)	68, 438, 787, 960, 1061, 1581, 1602, 1809
DpnI	GATC	9 cut(s)	285, 615, 693, 949, 1370, 1817, 1890, 2031, 2371
DpnII	GATC	9 cut(s)	283, 613, 691, 947, 1368, 1815, 1888, 2029, 2369
EaeI	YGGCCR	3 cut(s)	876, 1482, 2227
EagI	CGGCCG	1 cut(s)	2227
Eam1104I	CTCTTC	6 cut(s)	222, 317, 1524, 1731, 2149, 2400
EarI	CTCTTC	6 cut(s)	222, 317, 1524, 1731, 2149, 2400
EclXI	CGGCCG	1 cut(s)	2227
Eco130I	CCWWGG	4 cut(s)	57, 744, 997, 1270
Eco24I	GRGCYC	1 cut(s)	430
Eco31I	GGTCTC	1 cut(s)	2402
Eco47I	GGWCC	4 cut(s)	649, 964, 2147, 2393
Eco52I	CGGCCG	1 cut(s)	2227
Eco57I	CTGAAG	3 cut(s)	1297, 1425, 1467
Eco81I	CCTNAGG	1 cut(s)	960
Eco91I	GGTNACC	1 cut(s)	1772
EcoO109I	RGGNCCY	2 cut(s)	427, 964
EcoO65I	GGTNACC	1 cut(s)	1772
EcoRI	GAATTC	2 cut(s)	359, 2138
EcoRII	CCWGG	8 cut(s)	133, 1423, 1614, 1875, 2098, 2149, 2200, 2395
EcoT14I	CCWWGG	4 cut(s)	57, 744, 997, 1270
EcoT22I	ATGCAT	1 cut(s)	904
EcoT38I	GRGCYC	1 cut(s)	430
ErhI	CCWWGG	4 cut(s)	57, 744, 997, 1270
FalI	AAGNNNNNCTT	2 cut(s)	314, 346
FaqI	GGGAC	3 cut(s)	112, 156, 2217
FauI	CCCGC	3 cut(s)	803, 1709, 2340
FauNDI	CATATG	1 cut(s)	673
FblI	GTMKAC	1 cut(s)	2172
Fnu4HI	GCNGC	5 cut(s)	435, 481, 666, 1987, 2328
FokI	GGATG	8 cut(s)	483, 488, 889, 1031, 1432, 1472, 2040, 2303
FriOI	GRGCYC	1 cut(s)	430
Fsp4HI	GCNGC	5 cut(s)	435, 481, 666, 1987, 2328
FspBI	CTAG	3 cut(s)	998, 2033, 2120
GlaI	GCGC	2 cut(s)	366, 1715
GluI	GCNGC	5 cut(s)	435, 481, 666, 1987, 2328
GsuI	CTGGAG	2 cut(s)	117, 1229
HaeIII	GGCC	8 cut(s)	428, 767, 878, 1059, 1438, 1484, 2163, 2229
HapII	CCGG	2 cut(s)	583, 1471
HgaI	GACGC	1 cut(s)	1872
HhaI	GCGC	2 cut(s)	367, 1716
Hin1I	GRCGYC	1 cut(s)	1864
Hin6I	GCGC	2 cut(s)	365, 1714
HinP1I	GCGC	2 cut(s)	365, 1714
HinfI	GANTC	7 cut(s)	41, 70, 260, 325, 1094, 1606, 2321
HpaII	CCGG	2 cut(s)	583, 1471
HphI	GGTGA	3 cut(s)	1259, 1640, 2247
Hpy166II	GTNNAC	6 cut(s)	649, 685, 1027, 2062, 2147, 2173
Hpy188I	TCNGA	6 cut(s)	75, 706, 790, 1368, 1447, 2428
Hpy188III	TCNNGA	4 cut(s)	440, 951, 1610, 2210
Hpy8I	GTNNAC	6 cut(s)	649, 685, 1027, 2062, 2147, 2173
HpyAV	CCTTC	8 cut(s)	338, 493, 916, 1442, 1537, 1826, 2186, 2308
HpyCH4III	ACNGT	9 cut(s)	217, 419, 646, 728, 865, 1031, 1396, 1708, 2413
HpyCH4IV	ACGT	1 cut(s)	1047
HpyCH4V	TGCA	9 cut(s)	434, 665, 902, 1037, 1391, 1643, 1860, 1933, 2117
HpyF10VI	GCNNNNNNNGC	3 cut(s)	434, 443, 1065
HpyF3I	CTNAG	8 cut(s)	68, 438, 787, 960, 1061, 1581, 1602, 1809
HpySE526I	ACGT	1 cut(s)	1047
Hsp92I	GRCGYC	1 cut(s)	1864
HspAI	GCGC	2 cut(s)	365, 1714
Kzo9I	GATC	9 cut(s)	283, 613, 691, 947, 1368, 1815, 1888, 2029, 2369
LguI	GCTCTTC	1 cut(s)	1731
LmnI	GCTCC	7 cut(s)	160, 271, 316, 673, 754, 886, 1557
Lsp1109I	GCAGC	4 cut(s)	446, 677, 1998, 2339
LweI	GCATC	8 cut(s)	304, 807, 911, 1324, 1341, 1410, 1706, 2119
MabI	ACCWGGT	1 cut(s)	1875
MaeI	CTAG	3 cut(s)	998, 2033, 2120
MaeII	ACGT	1 cut(s)	1047
MaeIII	GTNAC	7 cut(s)	79, 919, 1048, 1246, 1628, 1772, 2090
MalI	GATC	9 cut(s)	285, 615, 693, 949, 1370, 1817, 1890, 2031, 2371
MboI	GATC	9 cut(s)	283, 613, 691, 947, 1368, 1815, 1888, 2029, 2369
MfeI	CAATTG	2 cut(s)	1620, 2360
MflI	RGATCY	4 cut(s)	691, 947, 2029, 2369
MhlI	GDGCHC	1 cut(s)	430
MlsI	TGGCCA	1 cut(s)	1484
MluNI	TGGCCA	1 cut(s)	1484
MlyI	GAGTC	5 cut(s)	50, 79, 334, 1615, 2315
MmeI	TCCRAC	4 cut(s)	138, 436, 754, 1215
Mox20I	TGGCCA	1 cut(s)	1484
Mph1103I	ATGCAT	1 cut(s)	904
MroXI	GAANNNNTTC	1 cut(s)	259
MscI	TGGCCA	1 cut(s)	1484
MslI	CAYNNNNRTG	4 cut(s)	597, 1032, 1269, 1280
Msp20I	TGGCCA	1 cut(s)	1484
MspA1I	CMGCKG	1 cut(s)	871
MspCI	CTTAAG	2 cut(s)	1195, 2242
MspI	CCGG	2 cut(s)	583, 1471
MspR9I	CCNGG	8 cut(s)	135, 1425, 1616, 1877, 2100, 2151, 2202, 2397
MunI	CAATTG	2 cut(s)	1620, 2360
Mva1269I	GAATGC	2 cut(s)	889, 1643
MvaI	CCWGG	8 cut(s)	135, 1425, 1616, 1877, 2100, 2151, 2202, 2397
MvnI	CGCG	3 cut(s)	365, 1716, 2263
MwoI	GCNNNNNNNGC	3 cut(s)	434, 443, 1065
NcoI	CCATGG	1 cut(s)	1270
NdeI	CATATG	1 cut(s)	673
NdeII	GATC	9 cut(s)	283, 613, 691, 947, 1368, 1815, 1888, 2029, 2369
NlaIV	GGNNCC	9 cut(s)	273, 428, 429, 651, 693, 750, 1874, 2208, 2371
NmeAIII	GCCGAG	1 cut(s)	2303
NmuCI	GTSAC	4 cut(s)	919, 1048, 1628, 2090
NsiI	ATGCAT	1 cut(s)	904
NspI	RCATGY	2 cut(s)	1681, 2271
PagI	TCATGA	1 cut(s)	1609
PciSI	GCTCTTC	1 cut(s)	1731
PctI	GAATGC	2 cut(s)	889, 1643
PdmI	GAANNNNTTC	1 cut(s)	259
PfeI	GAWTC	2 cut(s)	260, 1094
PflFI	GACNNNGTC	1 cut(s)	1431
PflMI	CCANNNNNTGG	2 cut(s)	677, 874
PfoI	TCCNGGA	1 cut(s)	2395
PkrI	GCNGC	5 cut(s)	436, 482, 667, 1988, 2329
PleI	GAGTC	5 cut(s)	49, 78, 333, 1614, 2315
PpsI	GAGTC	5 cut(s)	49, 78, 333, 1614, 2315
PpuMI	RGGWCCY	1 cut(s)	964
PsiI	TTATAA	1 cut(s)	1748
Psp5II	RGGWCCY	1 cut(s)	964
Psp6I	CCWGG	8 cut(s)	133, 1423, 1614, 1875, 2098, 2149, 2200, 2395
PspEI	GGTNACC	1 cut(s)	1772
PspGI	CCWGG	8 cut(s)	133, 1423, 1614, 1875, 2098, 2149, 2200, 2395
PspN4I	GGNNCC	9 cut(s)	273, 428, 429, 651, 693, 750, 1874, 2208, 2371
PspOMI	GGGCCC	1 cut(s)	426
PspPI	GGNCC	7 cut(s)	426, 427, 649, 766, 964, 2147, 2393
PspPPI	RGGWCCY	1 cut(s)	964
PstI	CTGCAG	1 cut(s)	436
PsuI	RGATCY	4 cut(s)	691, 947, 2029, 2369
PsyI	GACNNNGTC	1 cut(s)	1431
PvuII	CAGCTG	1 cut(s)	871
RsaI	GTAC	8 cut(s)	416, 686, 725, 1310, 1670, 2078, 2108, 2250
RsaNI	GTAC	8 cut(s)	415, 685, 724, 1309, 1669, 2077, 2107, 2249
RseI	CAYNNNNRTG	4 cut(s)	597, 1032, 1269, 1280
SapI	GCTCTTC	1 cut(s)	1731
SatI	GCNGC	5 cut(s)	435, 481, 666, 1987, 2328
Sau3AI	GATC	9 cut(s)	283, 613, 691, 947, 1368, 1815, 1888, 2029, 2369
Sau96I	GGNCC	7 cut(s)	426, 427, 649, 766, 964, 2147, 2393
ScaI	AGTACT	2 cut(s)	1310, 2250
SchI	GAGTC	5 cut(s)	50, 79, 334, 1615, 2315
ScrFI	CCNGG	8 cut(s)	135, 1425, 1616, 1877, 2100, 2151, 2202, 2397
SduI	GDGCHC	1 cut(s)	430
SexAI	ACCWGGT	1 cut(s)	1875
SfaNI	GCATC	8 cut(s)	304, 807, 911, 1324, 1341, 1410, 1706, 2119
SfcI	CTRYAG	1 cut(s)	432
SinI	GGWCC	4 cut(s)	649, 964, 2147, 2393
SmiMI	CAYNNNNRTG	4 cut(s)	597, 1032, 1269, 1280
SmlI	CTYRAG	2 cut(s)	1195, 2242
SmoI	CTYRAG	2 cut(s)	1195, 2242
SsiI	CCGC	6 cut(s)	480, 796, 1177, 1716, 2261, 2347
SspI	AATATT	1 cut(s)	1380
SspMI	CTAG	3 cut(s)	998, 2033, 2120
StyD4I	CCNGG	8 cut(s)	133, 1423, 1614, 1875, 2098, 2149, 2200, 2395
StyI	CCWWGG	4 cut(s)	57, 744, 997, 1270
TaaI	ACNGT	9 cut(s)	217, 419, 646, 728, 865, 1031, 1396, 1708, 2413
TaiI	ACGT	1 cut(s)	1050
TaqI	TCGA	7 cut(s)	112, 206, 298, 1234, 1538, 1818, 2284
TatI	WGTACW	5 cut(s)	684, 1308, 1668, 2106, 2248
TauI	GCSGC	1 cut(s)	483
TfiI	GAWTC	2 cut(s)	260, 1094
TscAI	CASTG	6 cut(s)	226, 649, 1057, 1069, 1321, 1585
TseFI	GTSAC	4 cut(s)	919, 1048, 1628, 2090
TseI	GCWGC	4 cut(s)	434, 665, 1986, 2327
Tsp45I	GTSAC	4 cut(s)	919, 1048, 1628, 2090
TspGWI	ACGGA	1 cut(s)	2362
TspRI	CASTG	6 cut(s)	226, 649, 1057, 1069, 1321, 1585
Tth111I	GACNNNGTC	1 cut(s)	1431
Van91I	CCANNNNNTGG	2 cut(s)	677, 874
Vha464I	CTTAAG	2 cut(s)	1195, 2242
VpaK11BI	GGWCC	4 cut(s)	649, 964, 2147, 2393
XapI	RAATTY	9 cut(s)	359, 380, 506, 578, 815, 1548, 1949, 2138, 2218
XceI	RCATGY	2 cut(s)	1681, 2271
XmaJI	CCTAGG	1 cut(s)	997
XmiI	GTMKAC	1 cut(s)	2172
XmnI	GAANNNNTTC	1 cut(s)	259
XspI	CTAG	3 cut(s)	998, 2033, 2120
ZrmI	AGTACT	2 cut(s)	1310, 2250
Zsp2I	ATGCAT	1 cut(s)	904

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.