Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6C

Orthogroup ID

OG0013627

Physical Location & Seq

Genomic Coordinates Reverse (-)

63141442 .. 63146509

Gene Structure 5068 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6CG456100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2448 bp

ATGATGTTGGGTTCTTTGGGTCTTCATCTCACTTGTTCTTTTGGGTCTTTATCGTGTTCCAAGGACTACCTGAGTCCGAGGGTAACTCAAACTAAAGCAGTCTCTTTTCAGCTTCGACATAGAGTATTGTCCCCTCCAGGGGGTTTTAGATGTGGGAAGTTGGAGCGTAGTCCCAGTCCCAGTCCCAGAGTTTCAGCAGTTTTGACAGACGACAGCTCGAAAATGACAGTCAGTGATGAAGAGATGGAAGATATTGGTGTTCTGAGAACAGATTCAAACTTGGAGCCATACAAAGATCACTTCAAGCATCGAATAACAAGATATTTGGAGCAGAAGAGTCTCTTTGAGAAATATGAAGGAGGTTTAGAGGAATTCGCGCAAGGTTATGTGAAATTTGGATTTAACAGAGAAGATGGTGGAATTGTGTACCGTGAATGGGCCCCTGCAGCTCAGGAGGCACAACTTGTTGGAGACTTTAATGGATGGGATGGCTGCAAATACGAGATGGAGAAGGGCGAATTTGGGGTATGGAGTATTAAGATACCCGATTTTGATGGAAGTCCAGCTATTCCTCACAATTCAAAGGTTAAATTCCGGTTCAAGCATGGAAATGGAGTTTGGGTTGATCGTATTCCTGCTTGGATTAAATATGCCACTGTGGACCCAACAAGATTTGCAGCTCCATATGATGGTGTGTACTGGGATCCACCACCATCAGAAAGGTATCAGTTTAAGTACGGTCGTCCTCCAAAACCCAAGGCTCCACGAATATATGAGGCCCATGTTGGAATGAGTAGCTCAGAACCCCGCATCAGTTCATACAGAGAATTTGCTGATAATGTTTTGCCTCGTATCCAAGCAAATAACTATAACACAGTCCAGCTGATGGCCGTGATGGAGCATTCCTATTATGCATCCTTCGGTTATCATGTGACAAACTTCTTTGCCGTAAGCAGTAGATCTGGAACACCTGAGGACCTTAAATATCTAATTGATAAAGCACATTCCCTAGGTTTGCGTGTATTGATGGATGTTGTTCACAGTCATGCAAGTAATAACGTCACTGATGGCCTCAGTGGCTTTGAAGTTGGTCAAAGCACACAAGATTCCTATTTCCACACAGGAGATAGAGGCTACCATAAGCTATGGGATAGCCGACTTTTCAACTATGCTAATTGGGAAGTTCTCCGCTTCTTTTTATCCAACTTAAGGTGGTGGCTGGAGGAGTATCAATTTGATGGGTTTCGATTTGATGGAGTAACTTCCATGCTGTATCATCACCATGGCATAAATATGGCATTTACAGGGAATTATGATGAGTACTTCAATGAGGCAACAGATGTTGATGCTGTTGTTTATTTGATGCTTGCCAACTATCTGATCCATAAAATATTGCCATCTGCAACTGTAATTGCTGAAGATGTTTCTGGGATGCCTGGACTTGGTCGGCCTGTCACTGAAGGAGGTGTTGGTTTTGACTACCGGCTGGCAATGGCCATCCCTGACAAATGGATTGATTATGTGAAAAACAAGAAAGACGAAGAGTGGTCGATGAAGGAAATTTCATGGAGCTTGACGAATAGGAGATACACTGAGAAGTGTGTTTCCTATGCTGAGAGTCATGACCAGGCAATTGTGGGTGACAAGACGATTGCATTCTTTTTGATGGATAAAGAAATGTACTCTGGCATGTCTTGTTTGAATGAAGCATCTCCTACTGTTGAGCGCGGGGTTGCTCTTCACAAGATGATACATTTTATAACTATGGCATTAGGAGGCGAGGGTTACCTTAACTTTATGGGTAATGAGTTTGGACACCCTGAGTGGATCGACTTTCCAAGAGAAGGCAATGAGTGGAGTTATGAAAAGTGCAGACGCCAATGGAACCTGGTGGATACAGATCATTTAAGATATAAGTTTATGAACGCATTTGACAGAGCTATGCACTTGCTTGATGAGAAATTTTCGTTCCTTTCATCAACGAAGCAGATAGTGAGCAGCACAAATGAAGAAGACAAGGTTATTGTCTTTGAGCGTGGAGATCTAGTTTTTGTATTTAATTTTCATCCAGTAAACACATACGATGGGTACAAAGTGGGGTGTGACTTACCTGGGAAGTACAGAGTTGCACTAGATAGTGATGCTTTTGAATTCGGTGGACCTGGAAGAGTAGGCCATGATGTAGACCACTTCACATTCCCAGAAGGAATACCAGGGGTTCCTGAAACAAATTTCAACGGCCGTCCCAACTCCTTAAGAGTACTCTCACCACCGCGAACATGCGTGGTTTATTATCGAGTGGATGAAAGTAAGGAGACTGCTGTGAAGGAGGACTCGGCAGCAGAAGTTGTGGTTGGGCGGGAGATCTCCCAATTGCTTGGATCCGTGAAAGATGGAAATGTTGGTCCAGGAGTGGAAGAGACCGTCAAAGTTTCGTCAGATAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003844 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0006073 GO:0008150 GO:0008152 GO:0009501 GO:0009507 GO:0009536 GO:0009987 GO:0016740 GO:0016757 GO:0016758 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase Alpha-amylase_C CBM_48

Protein Analysis

815

Amino Acids

92.73

Weight (kDa)

5.61

Isoelectric Point (pI)

33.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_48	PF02922	131 - 214	7.4e-20	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Alpha-amylase	PF00128	304 - 356	3.6e-09	Alpha amylase, catalytic domain
Alpha-amylase_C	PF02806	666 - 766	5e-20	Alpha amylase, C-terminal all-beta domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013627)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g37381 FvH4_2g37410
malus_domestica	MD08G1002300.v1.1
prunus_persica	Prupe.1G354000_v2.0.a1 Prupe.1G354000_v2.0.a1 Prupe.1G354000_v2.0.a1
pyrus_communis	pycom08g00150
rosa_chinensis	RchiOBHm_Chr6g0304311
rosa_laevigata	RLG00000010992
rosa_multiflora	Rmu_ssc0000218.1_g000037
rosa_roxburghii	Rroxscaffold_7G00163850
rosa_rugosa	Rorug06G0329900 Rorug06G0330000
rosa_samantha	Rh6AG443300 Rh6BG459100 Rh6CG456100 Rh6DG442800
rosa_wichuraiana	Rw6G038470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1760
AccB7I	CCANNNNNTGG	2 cut(s)	689, 886
AccI	GTMKAC	1 cut(s)	2184
AccII	CGCG	3 cut(s)	377, 1728, 2275
AciI	CCGC	5 cut(s)	808, 1189, 1728, 2273, 2359
AclWI	GGATC	6 cut(s)	698, 711, 1375, 1835, 2376, 2389
AcoI	YGGCCR	3 cut(s)	888, 1494, 2239
AcsI	RAATTY	9 cut(s)	371, 392, 518, 590, 827, 1560, 1961, 2150, 2230
AcuI	CTGAAG	2 cut(s)	1437, 1479
AcyI	GRCGYC	1 cut(s)	1876
AfaI	GTAC	8 cut(s)	428, 698, 737, 1322, 1682, 2090, 2120, 2262
AfiI	CCNNNNNNNGG	9 cut(s)	138, 139, 140, 436, 689, 886, 1209, 1442, 1844
AflII	CTTAAG	2 cut(s)	1207, 2254
AjnI	CCWGG	8 cut(s)	136, 1435, 1626, 1887, 2110, 2161, 2212, 2407
AjuI	GAANNNNNNNTTGG	6 cut(s)	601, 633, 1452, 1484, 2337, 2369
Alw26I	GTCTC	5 cut(s)	106, 344, 465, 2309, 2414
AlwI	GGATC	6 cut(s)	698, 711, 1375, 1835, 2376, 2389
AoxI	GGCC	8 cut(s)	438, 777, 888, 1069, 1448, 1494, 2173, 2239
ApaI	GGGCCC	1 cut(s)	442
ApeKI	GCWGC	5 cut(s)	446, 492, 677, 1998, 2339
ApoI	RAATTY	9 cut(s)	371, 392, 518, 590, 827, 1560, 1961, 2150, 2230
Asp700I	GAANNNNTTC	1 cut(s)	271
AspA2I	CCTAGG	1 cut(s)	1009
AspLEI	GCGC	2 cut(s)	379, 1728
AspS9I	GGNCC	7 cut(s)	438, 439, 661, 778, 976, 2159, 2405
AsuHPI	GGTGA	3 cut(s)	1271, 1652, 2259
AvaII	GGWCC	4 cut(s)	661, 976, 2159, 2405
AvrII	CCTAGG	1 cut(s)	1009
AxyI	CCTNAGG	1 cut(s)	972
BaeGI	GKGCMC	1 cut(s)	442
BalI	TGGCCA	1 cut(s)	1496
BamHI	GGATCC	2 cut(s)	703, 2381
BanII	GRGCYC	1 cut(s)	442
BbsI	GAAGAC	2 cut(s)	14, 2019
BbvI	GCAGC	5 cut(s)	458, 479, 689, 2010, 2351
BceAI	ACGGC	4 cut(s)	875, 932, 2226, 2254
BciT130I	CCWGG	8 cut(s)	138, 1437, 1628, 1889, 2112, 2163, 2214, 2409
BciVI	GTATCC	2 cut(s)	863, 1888
BcoDI	GTCTC	5 cut(s)	106, 344, 465, 2309, 2414
BfaI	CTAG	3 cut(s)	1010, 2045, 2132
BfmI	CTRYAG	1 cut(s)	444
BfrI	CTTAAG	2 cut(s)	1207, 2254
BfuI	GTATCC	2 cut(s)	863, 1888
BglI	GCCNNNNNGGC	1 cut(s)	1077
BglII	AGATCT	3 cut(s)	959, 2041, 2364
BisI	GCNGC	5 cut(s)	447, 493, 678, 1999, 2340
BlnI	CCTAGG	1 cut(s)	1009
BlsI	GCNGC	5 cut(s)	448, 494, 679, 2000, 2341
BmcAI	AGTACT	2 cut(s)	1322, 2262
Bme1390I	CCNGG	8 cut(s)	138, 1437, 1628, 1889, 2112, 2163, 2214, 2409
Bme18I	GGWCC	4 cut(s)	661, 976, 2159, 2405
BmgT120I	GGNCC	7 cut(s)	438, 439, 661, 778, 976, 2159, 2405
BmiI	GGNNCC	9 cut(s)	285, 440, 441, 663, 705, 762, 1886, 2220, 2383
BmrFI	CCNGG	8 cut(s)	138, 1437, 1628, 1889, 2112, 2163, 2214, 2409
BmrI	ACTGGG	3 cut(s)	168, 174, 709
BmsI	GCATC	8 cut(s)	316, 819, 923, 1336, 1353, 1422, 1718, 2131
BmuI	ACTGGG	3 cut(s)	168, 174, 709
BpiI	GAAGAC	2 cut(s)	14, 2019
BplI	GAGNNNNNCTC	2 cut(s)	70, 102
BpmI	CTGGAG	2 cut(s)	120, 1241
Bpu10I	CCTNAGC	1 cut(s)	450
BsaBI	GATNNNNATC	1 cut(s)	1899
BsaHI	GRCGYC	1 cut(s)	1876
BsaI	GGTCTC	1 cut(s)	2414
BsaJI	CCNNGG	8 cut(s)	60, 77, 137, 756, 1009, 1282, 2111, 2213
BsaWI	WCCGGW	1 cut(s)	594
BsaXI	ACNNNNNCTCC	4 cut(s)	606, 636, 2031, 2061
Bsc4I	CCNNNNNNNGG	9 cut(s)	138, 139, 140, 436, 689, 886, 1209, 1442, 1844
Bse118I	RCCGGY	1 cut(s)	1482
Bse1I	ACTGG	4 cut(s)	174, 180, 704, 2069
Bse21I	CCTNAGG	1 cut(s)	972
Bse3DI	GCAATG	2 cut(s)	1497, 1855
Bse8I	GATNNNNATC	1 cut(s)	1899
BseBI	CCWGG	8 cut(s)	138, 1437, 1628, 1889, 2112, 2163, 2214, 2409
BseDI	CCNNGG	8 cut(s)	60, 77, 137, 756, 1009, 1282, 2111, 2213
BseGI	GGATG	8 cut(s)	488, 493, 914, 1036, 1437, 1497, 2065, 2308
BseJI	GATNNNNATC	1 cut(s)	1899
BseLI	CCNNNNNNNGG	9 cut(s)	138, 139, 140, 436, 689, 886, 1209, 1442, 1844
BseMI	GCAATG	2 cut(s)	1497, 1855
BseMII	CTCAG	9 cut(s)	62, 254, 464, 813, 963, 1087, 1584, 1605, 1812
BseNI	ACTGG	4 cut(s)	174, 180, 704, 2069
BseRI	GAGGAG	1 cut(s)	1238
BseSI	GKGCMC	1 cut(s)	442
BseX3I	CGGCCG	1 cut(s)	2239
BseXI	GCAGC	5 cut(s)	458, 479, 689, 2010, 2351
BsgI	GTGCAG	1 cut(s)	1891
Bsh1236I	CGCG	3 cut(s)	377, 1728, 2275
Bsh1285I	CGRYCG	2 cut(s)	742, 2242
BshFI	GGCC	8 cut(s)	440, 779, 890, 1071, 1450, 1496, 2175, 2241
BsiEI	CGRYCG	2 cut(s)	742, 2242
BsiSI	CCGG	2 cut(s)	595, 1483
BslFI	GGGAC	5 cut(s)	115, 156, 162, 168, 2229
BslI	CCNNNNNNNGG	9 cut(s)	138, 139, 140, 436, 689, 886, 1209, 1442, 1844
BsmAI	GTCTC	5 cut(s)	106, 344, 465, 2309, 2414
BsmFI	GGGAC	5 cut(s)	115, 156, 162, 168, 2229
BsmI	GAATGC	2 cut(s)	901, 1655
BsnI	GGCC	8 cut(s)	440, 779, 890, 1071, 1450, 1496, 2175, 2241
Bso31I	GGTCTC	1 cut(s)	2414
Bsp120I	GGGCCC	1 cut(s)	438
Bsp1286I	GDGCHC	1 cut(s)	442
Bsp19I	CCATGG	1 cut(s)	1282
BspACI	CCGC	5 cut(s)	808, 1189, 1728, 2273, 2359
BspANI	GGCC	8 cut(s)	440, 779, 890, 1071, 1450, 1496, 2175, 2241
BspCNI	CTCAG	9 cut(s)	63, 255, 463, 812, 964, 1086, 1585, 1606, 1813
BspFNI	CGCG	3 cut(s)	377, 1728, 2275
BspHI	TCATGA	1 cut(s)	1621
BspLI	GGNNCC	9 cut(s)	285, 440, 441, 663, 705, 762, 1886, 2220, 2383
BspMAI	CTGCAG	1 cut(s)	448
BspPI	GGATC	6 cut(s)	698, 711, 1375, 1835, 2376, 2389
BspQI	GCTCTTC	1 cut(s)	1743
BspTI	CTTAAG	2 cut(s)	1207, 2254
BspTNI	GGTCTC	1 cut(s)	2414
BsrDI	GCAATG	2 cut(s)	1497, 1855
BsrFI	RCCGGY	1 cut(s)	1482
BsrI	ACTGG	4 cut(s)	174, 180, 704, 2069
BssAI	RCCGGY	1 cut(s)	1482
BssECI	CCNNGG	8 cut(s)	60, 77, 137, 756, 1009, 1282, 2111, 2213
BssNI	GRCGYC	1 cut(s)	1876
BssT1I	CCWWGG	4 cut(s)	60, 756, 1009, 1282
Bst2UI	CCWGG	8 cut(s)	138, 1437, 1628, 1889, 2112, 2163, 2214, 2409
Bst4CI	ACNGT	9 cut(s)	229, 431, 658, 740, 877, 1043, 1408, 1720, 2425
Bst6I	CTCTTC	6 cut(s)	234, 329, 1536, 1743, 2161, 2412
BstACI	GRCGYC	1 cut(s)	1876
BstAFI	CTTAAG	2 cut(s)	1207, 2254
BstC8I	GCNNGC	2 cut(s)	1368, 1488
BstDEI	CTNAG	9 cut(s)	71, 263, 450, 799, 972, 1073, 1593, 1614, 1821
BstDSI	CCRYGG	1 cut(s)	1282
BstEII	GGTNACC	1 cut(s)	1784
BstF5I	GGATG	8 cut(s)	488, 493, 914, 1036, 1437, 1497, 2065, 2308
BstFNI	CGCG	3 cut(s)	377, 1728, 2275
BstHHI	GCGC	2 cut(s)	379, 1728
BstMAI	GTCTC	5 cut(s)	106, 344, 465, 2309, 2414
BstMCI	CGRYCG	2 cut(s)	742, 2242
BstMWI	GCNNNNNNNGC	3 cut(s)	446, 455, 1077
BstNI	CCWGG	8 cut(s)	138, 1437, 1628, 1889, 2112, 2163, 2214, 2409
BstNSI	RCATGY	2 cut(s)	1693, 2283
BstPI	GGTNACC	1 cut(s)	1784
BstSCI	CCNGG	8 cut(s)	136, 1435, 1626, 1887, 2110, 2161, 2212, 2407
BstSFI	CTRYAG	1 cut(s)	444
BstSLI	GKGCMC	1 cut(s)	442
BstUI	CGCG	3 cut(s)	377, 1728, 2275
BstV1I	GCAGC	5 cut(s)	458, 479, 689, 2010, 2351
BstV2I	GAAGAC	2 cut(s)	14, 2019
BstX2I	RGATCY	5 cut(s)	703, 959, 2041, 2364, 2381
BstXI	CCANNNNNNTGG	1 cut(s)	2378
BstYI	RGATCY	5 cut(s)	703, 959, 2041, 2364, 2381
BstZI	CGGCCG	1 cut(s)	2239
Bsu36I	CCTNAGG	1 cut(s)	972
BsuI	GTATCC	2 cut(s)	863, 1888
BsuRI	GGCC	8 cut(s)	440, 779, 890, 1071, 1450, 1496, 2175, 2241
BtgI	CCRYGG	1 cut(s)	1282
BtsCI	GGATG	8 cut(s)	488, 493, 914, 1036, 1437, 1497, 2065, 2308
BtsIMutI	CAGTG	6 cut(s)	238, 654, 1062, 1081, 1455, 1590
Cac8I	GCNNGC	2 cut(s)	1368, 1488
CciI	TCATGA	1 cut(s)	1621
CfoI	GCGC	2 cut(s)	379, 1728
Cfr10I	RCCGGY	1 cut(s)	1482
Cfr13I	GGNCC	7 cut(s)	438, 439, 661, 778, 976, 2159, 2405
CseI	GACGC	1 cut(s)	1884
CsiI	ACCWGGT	1 cut(s)	1887
Csp6I	GTAC	8 cut(s)	427, 697, 736, 1321, 1681, 2089, 2119, 2261
CviQI	GTAC	8 cut(s)	427, 697, 736, 1321, 1681, 2089, 2119, 2261
DdeI	CTNAG	9 cut(s)	71, 263, 450, 799, 972, 1073, 1593, 1614, 1821
EaeI	YGGCCR	3 cut(s)	888, 1494, 2239
EagI	CGGCCG	1 cut(s)	2239
Eam1104I	CTCTTC	6 cut(s)	234, 329, 1536, 1743, 2161, 2412
EarI	CTCTTC	6 cut(s)	234, 329, 1536, 1743, 2161, 2412
EclXI	CGGCCG	1 cut(s)	2239
Eco130I	CCWWGG	4 cut(s)	60, 756, 1009, 1282
Eco24I	GRGCYC	1 cut(s)	442
Eco31I	GGTCTC	1 cut(s)	2414
Eco47I	GGWCC	4 cut(s)	661, 976, 2159, 2405
Eco52I	CGGCCG	1 cut(s)	2239
Eco57I	CTGAAG	2 cut(s)	1437, 1479
Eco81I	CCTNAGG	1 cut(s)	972
Eco91I	GGTNACC	1 cut(s)	1784
EcoO109I	RGGNCCY	2 cut(s)	439, 976
EcoO65I	GGTNACC	1 cut(s)	1784
EcoRI	GAATTC	2 cut(s)	371, 2150
EcoRII	CCWGG	8 cut(s)	136, 1435, 1626, 1887, 2110, 2161, 2212, 2407
EcoT14I	CCWWGG	4 cut(s)	60, 756, 1009, 1282
EcoT22I	ATGCAT	1 cut(s)	916
EcoT38I	GRGCYC	1 cut(s)	442
ErhI	CCWWGG	4 cut(s)	60, 756, 1009, 1282
FalI	AAGNNNNNCTT	2 cut(s)	326, 358
FaqI	GGGAC	5 cut(s)	115, 156, 162, 168, 2229
FauI	CCCGC	3 cut(s)	815, 1721, 2352
FauNDI	CATATG	1 cut(s)	685
FblI	GTMKAC	1 cut(s)	2184
Fnu4HI	GCNGC	5 cut(s)	447, 493, 678, 1999, 2340
FokI	GGATG	8 cut(s)	495, 500, 901, 1043, 1444, 1484, 2052, 2315
FriOI	GRGCYC	1 cut(s)	442
Fsp4HI	GCNGC	5 cut(s)	447, 493, 678, 1999, 2340
FspBI	CTAG	3 cut(s)	1010, 2045, 2132
GlaI	GCGC	2 cut(s)	378, 1727
GluI	GCNGC	5 cut(s)	447, 493, 678, 1999, 2340
GsuI	CTGGAG	2 cut(s)	120, 1241
HaeIII	GGCC	8 cut(s)	440, 779, 890, 1071, 1450, 1496, 2175, 2241
HapII	CCGG	2 cut(s)	595, 1483
HgaI	GACGC	1 cut(s)	1884
HhaI	GCGC	2 cut(s)	379, 1728
Hin1I	GRCGYC	1 cut(s)	1876
Hin6I	GCGC	2 cut(s)	377, 1726
HinP1I	GCGC	2 cut(s)	377, 1726
HinfI	GANTC	6 cut(s)	73, 272, 337, 1106, 1618, 2333
HpaII	CCGG	2 cut(s)	595, 1483
HphI	GGTGA	3 cut(s)	1271, 1652, 2259
Hpy166II	GTNNAC	7 cut(s)	427, 661, 697, 1039, 2074, 2159, 2185
Hpy188I	TCNGA	6 cut(s)	78, 264, 718, 802, 1380, 2440
Hpy188III	TCNNGA	4 cut(s)	452, 963, 1622, 2222
Hpy8I	GTNNAC	7 cut(s)	427, 661, 697, 1039, 2074, 2159, 2185
HpyAV	CCTTC	8 cut(s)	350, 505, 928, 1454, 1549, 1838, 2198, 2320
HpyCH4III	ACNGT	9 cut(s)	229, 431, 658, 740, 877, 1043, 1408, 1720, 2425
HpyCH4IV	ACGT	1 cut(s)	1059
HpyF10VI	GCNNNNNNNGC	3 cut(s)	446, 455, 1077
HpyF3I	CTNAG	9 cut(s)	71, 263, 450, 799, 972, 1073, 1593, 1614, 1821
HpySE526I	ACGT	1 cut(s)	1059
Hsp92I	GRCGYC	1 cut(s)	1876
HspAI	GCGC	2 cut(s)	377, 1726
LguI	GCTCTTC	1 cut(s)	1743
LmnI	GCTCC	7 cut(s)	163, 283, 328, 685, 766, 898, 1569
Lsp1109I	GCAGC	5 cut(s)	458, 479, 689, 2010, 2351
LweI	GCATC	8 cut(s)	316, 819, 923, 1336, 1353, 1422, 1718, 2131
MabI	ACCWGGT	1 cut(s)	1887
MaeI	CTAG	3 cut(s)	1010, 2045, 2132
MaeII	ACGT	1 cut(s)	1059
MaeIII	GTNAC	8 cut(s)	82, 931, 1060, 1258, 1453, 1640, 1784, 2102
MfeI	CAATTG	2 cut(s)	1632, 2372
MflI	RGATCY	5 cut(s)	703, 959, 2041, 2364, 2381
MhlI	GDGCHC	1 cut(s)	442
MlsI	TGGCCA	1 cut(s)	1496
MluNI	TGGCCA	1 cut(s)	1496
MlyI	GAGTC	4 cut(s)	82, 346, 1627, 2327
MmeI	TCCRAC	4 cut(s)	141, 448, 766, 1227
Mox20I	TGGCCA	1 cut(s)	1496
Mph1103I	ATGCAT	1 cut(s)	916
MroXI	GAANNNNTTC	1 cut(s)	271
MscI	TGGCCA	1 cut(s)	1496
MslI	CAYNNNNRTG	4 cut(s)	609, 1044, 1281, 1292
Msp20I	TGGCCA	1 cut(s)	1496
MspA1I	CMGCKG	1 cut(s)	883
MspCI	CTTAAG	2 cut(s)	1207, 2254
MspI	CCGG	2 cut(s)	595, 1483
MspR9I	CCNGG	8 cut(s)	138, 1437, 1628, 1889, 2112, 2163, 2214, 2409
MunI	CAATTG	2 cut(s)	1632, 2372
Mva1269I	GAATGC	2 cut(s)	901, 1655
MvaI	CCWGG	8 cut(s)	138, 1437, 1628, 1889, 2112, 2163, 2214, 2409
MvnI	CGCG	3 cut(s)	377, 1728, 2275
MwoI	GCNNNNNNNGC	3 cut(s)	446, 455, 1077
NcoI	CCATGG	1 cut(s)	1282
NdeI	CATATG	1 cut(s)	685
NlaIV	GGNNCC	9 cut(s)	285, 440, 441, 663, 705, 762, 1886, 2220, 2383
NmeAIII	GCCGAG	1 cut(s)	2315
NmuCI	GTSAC	5 cut(s)	931, 1060, 1453, 1640, 2102
NsiI	ATGCAT	1 cut(s)	916
NspI	RCATGY	2 cut(s)	1693, 2283
PagI	TCATGA	1 cut(s)	1621
PciSI	GCTCTTC	1 cut(s)	1743
PctI	GAATGC	2 cut(s)	901, 1655
PdmI	GAANNNNTTC	1 cut(s)	271
PfeI	GAWTC	2 cut(s)	272, 1106
PflFI	GACNNNGTC	1 cut(s)	1443
PflMI	CCANNNNNTGG	2 cut(s)	689, 886
PfoI	TCCNGGA	1 cut(s)	2407
PkrI	GCNGC	5 cut(s)	448, 494, 679, 2000, 2341
PleI	GAGTC	4 cut(s)	81, 345, 1626, 2327
PpsI	GAGTC	4 cut(s)	81, 345, 1626, 2327
PpuMI	RGGWCCY	1 cut(s)	976
PsiI	TTATAA	1 cut(s)	1760
Psp5II	RGGWCCY	1 cut(s)	976
Psp6I	CCWGG	8 cut(s)	136, 1435, 1626, 1887, 2110, 2161, 2212, 2407
PspEI	GGTNACC	1 cut(s)	1784
PspGI	CCWGG	8 cut(s)	136, 1435, 1626, 1887, 2110, 2161, 2212, 2407
PspN4I	GGNNCC	9 cut(s)	285, 440, 441, 663, 705, 762, 1886, 2220, 2383
PspOMI	GGGCCC	1 cut(s)	438
PspPI	GGNCC	7 cut(s)	438, 439, 661, 778, 976, 2159, 2405
PspPPI	RGGWCCY	1 cut(s)	976
PstI	CTGCAG	1 cut(s)	448
PsuI	RGATCY	5 cut(s)	703, 959, 2041, 2364, 2381
PsyI	GACNNNGTC	1 cut(s)	1443
PvuII	CAGCTG	1 cut(s)	883
RsaI	GTAC	8 cut(s)	428, 698, 737, 1322, 1682, 2090, 2120, 2262
RsaNI	GTAC	8 cut(s)	427, 697, 736, 1321, 1681, 2089, 2119, 2261
RseI	CAYNNNNRTG	4 cut(s)	609, 1044, 1281, 1292
SapI	GCTCTTC	1 cut(s)	1743
SatI	GCNGC	5 cut(s)	447, 493, 678, 1999, 2340
Sau96I	GGNCC	7 cut(s)	438, 439, 661, 778, 976, 2159, 2405
ScaI	AGTACT	2 cut(s)	1322, 2262
SchI	GAGTC	4 cut(s)	82, 346, 1627, 2327
ScrFI	CCNGG	8 cut(s)	138, 1437, 1628, 1889, 2112, 2163, 2214, 2409
SduI	GDGCHC	1 cut(s)	442
SexAI	ACCWGGT	1 cut(s)	1887
SfaNI	GCATC	8 cut(s)	316, 819, 923, 1336, 1353, 1422, 1718, 2131
SfcI	CTRYAG	1 cut(s)	444
SinI	GGWCC	4 cut(s)	661, 976, 2159, 2405
SmiMI	CAYNNNNRTG	4 cut(s)	609, 1044, 1281, 1292
SmlI	CTYRAG	2 cut(s)	1207, 2254
SmoI	CTYRAG	2 cut(s)	1207, 2254
SsiI	CCGC	5 cut(s)	808, 1189, 1728, 2273, 2359
SspI	AATATT	1 cut(s)	1392
SspMI	CTAG	3 cut(s)	1010, 2045, 2132
StyD4I	CCNGG	8 cut(s)	136, 1435, 1626, 1887, 2110, 2161, 2212, 2407
StyI	CCWWGG	4 cut(s)	60, 756, 1009, 1282
TaaI	ACNGT	9 cut(s)	229, 431, 658, 740, 877, 1043, 1408, 1720, 2425
TaiI	ACGT	1 cut(s)	1062
TaqI	TCGA	7 cut(s)	115, 218, 310, 1246, 1550, 1830, 2296
TatI	WGTACW	5 cut(s)	696, 1320, 1680, 2118, 2260
TfiI	GAWTC	2 cut(s)	272, 1106
TscAI	CASTG	6 cut(s)	238, 661, 1069, 1081, 1462, 1597
TseFI	GTSAC	5 cut(s)	931, 1060, 1453, 1640, 2102
TseI	GCWGC	5 cut(s)	446, 492, 677, 1998, 2339
Tsp45I	GTSAC	5 cut(s)	931, 1060, 1453, 1640, 2102
TspGWI	ACGGA	1 cut(s)	2374
TspRI	CASTG	6 cut(s)	238, 661, 1069, 1081, 1462, 1597
Tth111I	GACNNNGTC	1 cut(s)	1443
Van91I	CCANNNNNTGG	2 cut(s)	689, 886
Vha464I	CTTAAG	2 cut(s)	1207, 2254
VpaK11BI	GGWCC	4 cut(s)	661, 976, 2159, 2405
XapI	RAATTY	9 cut(s)	371, 392, 518, 590, 827, 1560, 1961, 2150, 2230
XceI	RCATGY	2 cut(s)	1693, 2283
XmaJI	CCTAGG	1 cut(s)	1009
XmiI	GTMKAC	1 cut(s)	2184
XmnI	GAANNNNTTC	1 cut(s)	271
XspI	CTAG	3 cut(s)	1010, 2045, 2132
ZrmI	AGTACT	2 cut(s)	1322, 2262
Zsp2I	ATGCAT	1 cut(s)	916

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.