Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr8

Orthogroup ID

OG0013627

Physical Location & Seq

Genomic Coordinates Forward (+)

148848 .. 154453

Gene Structure 5606 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom08g00150.4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2514 bp

ATGATGATGTTGGCACCGACTGGTTTGGTTCCTCGTCATCCCACTCTCACTCCTCTATCATTTACCTTTACGAGCACCAACGACGAGTACCCATCCAGACTCGGGCTTGGACTCAGGGGCAAAGAGCAGCAAGCAGTGCGGTTGTTGAGGTTCGGAACATCTCAAAACTCATCATCGCATCTTCGCAGACGAGGTTCCCCCTCAACAACAAGAAGTAGATGTTCCAAGGCAGAGCCTACTTCCAAAAGTAAAGCTGTTTTCATAGACGACAGCCCCATAATAACAGACACCGACCAAGGCATGGAAAGCCTTGGAATCTTGAGCATCGATCAATCCTTGCAACCACACAAGGACCACTTCAACTATAGAATCAACAGATACCTGGATCAGAGAAGGCTTATTGAAACGTACGAAGGAGGTCTACAGGAATTTGCTCAAGGTTATTTGAAATTTGGATTTAACAGAGAAGAAGGTGGAATTGTGTACCGTGAATGGGCCCCTGCCGCTCAGGAGGCACAACTTATTGGGGACTTTAATGGATGGGATGGCTCCAAACACAAGATGGACAAGAACCAATTTGGGGTTTGGAGTATCAAGGTACCCGATTCTGGTGGAAATTCAGCCATTCCTCACAATTCAAGGGTTAAATTCCGGTTCAAGCATGGAGATGGAGTTTGGGTTGATCGCATCCCTGCTTGGATTCAATATGCCACCGTGGACCCAGCAAGGTTTGCAGCACCTTATGATGGTGTCTACTGGGATCCACCACCATCAGAAAGGTTTCAGTTCAAGTATCCTCGTCCTCCAAAACCCAAGGCTCCACGAGTATATGAGGCACATGTTGGAATGAGTAGCTCAGAACCCCGCATCAGTTCATATAGAGAATTTGCTGATGACGTTTTACCGCGTATCCAAGCAAATAACTATAACACAGTCCAGTTGATGGCCGTGATGGAGCATTCCTATTATGCATCATTCGGTTATCATGTGACAAACTTCTTTGCCGTCAGCAGTAGATCTGGAACACCTGAAGACCTTAAATATCTAGTTGACAAAGCCCATTCCCTAGGTTTGCGGGTATTGATGGATGTTGTTCACAGCCATGCAAGTAACAACATTACTGATGGCCTCAATGGCTTTGAAGTTGGTCAAAGCTCACAAGAGTCCTACTTCCACACAGGAGATAGAGGCTATCATAAGTTATGGGATAGCCGACTTTTCAACTATGCTAATTGGGAAGTTCTCCGCTTCCTTTTATCCAACTTGAGGTGGTGGCTGGAGGAGTTTAAATTTGATGGGTTTCGATTTGATGGAGTAACTTCAATGTTATATCATCACCACGGGATAAACATGGCATTTTCAGGGGATTATCATGAGTATTTTAGTGAGGCAACAGATGTTGATGCTGTTGTTTATTTGATGCTTGCCAACCATCTGATCCATAAAGTTTTGCCAGATGCAACTGTAATTGCCGAAGATGTTTCTGGTATGCCCGGACTTGGTCGGCCTGTCTCTGAAGGTGGCATTGGCTTTGATTACCGGCTGGCAATGGCCATCCCTGACAAATGGATTGACTATGTGAAAAACAAGAGTGACGAAGAGTGGTCAATGAAGGAAATTTCATGGAACTTGACAAATAGGAGGTACACTGAGAAGTGTATCTCCTATGCTGAAAGTCACGATCAGGCAATTGTGGGTGACAAGACAATTGCATTCTTTTTGATGGATAGAGAAATGTATTCTGGCATGTCTTGTTTAGTTGATGCTTCTCCTACCATTGAGCGAGGGGTTGCGCTTCACAAGATGATACATTTCTTGACTATGGCATTAGGTGGCGAGGGGTATCTCAATTTTATGGGAAATGAGTTTGGCCATCCTGAATGGATTGACTTTCCAAGAGAAGGCAACGGGTGGAGTTATGAGAAGTGCAGACGCCAGTGGAACCTGGTGGATACAGATCATTTGAGATATAAGTTTATGAATGCCTTTGACAAAGCTATGAATTTGCTTGATGAGAAGTTTTCATTCCTTTCGTCAACAAAGCAGATAGTGAGCAGCACAAATGAAGAAGACAAGGTTATTGTCTTTGAGCGCGGGGATTTAGTTTTTGTGTTCAATTTTCATCCAAAAAACACATACGAAGGGTATAAAGTTGGGTGTGACTTACCTGGGAAGTACAGAGTTGCACTAGATAGTGATGCTTGGGAGTTTGGTGGCCATGGAAGAGTGGGCCATGATGTAGACCACTTCACATTCCCAGAAGGAATACCGGGGGTTCCTGAAACAAATTTCAACAACCGTCCCAACTCCTTCAAAATACTCTCACCGGCTCAAACATGTGTGGTTTATTATCGAGTGGATGAAAGTGAGGAGGCAGATGCGGAGGAGACATCAATAGAAGAGGAAGTTGGGGTTGGGCAGGAGAACTTTGAAGAACAAACTGGTCCCATGAATGATGACAATGCTGTTGGTCCAAGAGCACAAGAGAGTGACCAAGGGTCATCGTCGGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003844 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0006073 GO:0008150 GO:0008152 GO:0009501 GO:0009507 GO:0009536 GO:0009987 GO:0016740 GO:0016757 GO:0016758 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase Alpha-amylase_C CBM_48

Protein Analysis

838

Amino Acids

95.32

Weight (kDa)

5.48

Isoelectric Point (pI)

39.31

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013627)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g37381 FvH4_2g37410
malus_domestica	MD08G1002300.v1.1
prunus_persica	Prupe.1G354000_v2.0.a1 Prupe.1G354000_v2.0.a1 Prupe.1G354000_v2.0.a1
pyrus_communis	pycom08g00150
rosa_chinensis	RchiOBHm_Chr6g0304311
rosa_laevigata	RLG00000010992
rosa_multiflora	Rmu_ssc0000218.1_g000037
rosa_roxburghii	Rroxscaffold_7G00163850
rosa_rugosa	Rorug06G0329900 Rorug06G0330000
rosa_samantha	Rh6AG443300 Rh6BG459100 Rh6CG456100 Rh6DG442800
rosa_wichuraiana	Rw6G038470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	598
AccB1I	GGYRCC	2 cut(s)	13, 598
AccB7I	CCANNNNNTGG	2 cut(s)	301, 943
AccBSI	CCGCTC	1 cut(s)	506
AccI	GTMKAC	3 cut(s)	421, 753, 2241
AccII	CGCG	2 cut(s)	907, 2094
AciI	CCGC	8 cut(s)	139, 504, 865, 905, 1075, 1246, 2094, 2381
AclWI	GGATC	4 cut(s)	393, 755, 768, 1432
AcoI	YGGCCR	4 cut(s)	945, 1551, 1870, 2215
AcsI	RAATTY	9 cut(s)	428, 449, 616, 647, 884, 1289, 1617, 2002, 2287
AcuI	CTGAAG	2 cut(s)	1050, 1536
AcyI	GRCGYC	1 cut(s)	1933
AfaI	GTAC	6 cut(s)	89, 410, 485, 600, 1646, 2177
AflIII	ACRYGT	2 cut(s)	838, 2336
AhdI	GACNNNNNGTC	1 cut(s)	2497
AjnI	CCWGG	3 cut(s)	381, 1944, 2167
AjuI	GAANNNNNNNTTGG	6 cut(s)	1509, 1541, 2391, 2397, 2423, 2429
AluBI	AGCT	4 cut(s)	254, 855, 1155, 1997
AluI	AGCT	4 cut(s)	254, 855, 1155, 1997
Alw21I	GWGCWC	2 cut(s)	77, 2482
Alw26I	GTCTC	2 cut(s)	1516, 2381
AlwI	GGATC	4 cut(s)	393, 755, 768, 1432
Ama87I	CYCGRG	1 cut(s)	101
AoxI	GGCC	8 cut(s)	495, 945, 1126, 1505, 1551, 1870, 2215, 2230
ApaI	GGGCCC	1 cut(s)	499
ApeKI	GCWGC	3 cut(s)	127, 734, 2055
ApoI	RAATTY	9 cut(s)	428, 449, 616, 647, 884, 1289, 1617, 2002, 2287
Asp700I	GAANNNNTTC	1 cut(s)	780
Asp718I	GGTACC	1 cut(s)	598
AspA2I	CCTAGG	1 cut(s)	1066
AspLEI	GCGC	2 cut(s)	1795, 2094
AspS9I	GGNCC	7 cut(s)	352, 495, 496, 718, 2230, 2444, 2471
AsuC2I	CCSGG	2 cut(s)	1494, 2271
AsuHPI	GGTGA	3 cut(s)	1328, 1709, 2316
AvaI	CYCGRG	1 cut(s)	101
AvaII	GGWCC	4 cut(s)	352, 718, 2444, 2471
AvrII	CCTAGG	1 cut(s)	1066
BaeGI	GKGCMC	1 cut(s)	499
BalI	TGGCCA	3 cut(s)	1553, 1872, 2217
BamHI	GGATCC	1 cut(s)	760
BanI	GGYRCC	2 cut(s)	13, 598
BanII	GRGCYC	1 cut(s)	499
BarI	GAAGNNNNNNTAC	2 cut(s)	405, 437
BauI	CACGAG	1 cut(s)	822
BbsI	GAAGAC	2 cut(s)	1038, 2076
Bbv12I	GWGCWC	2 cut(s)	77, 2482
BbvI	GCAGC	3 cut(s)	139, 746, 2067
BceAI	ACGGC	2 cut(s)	932, 989
BciT130I	CCWGG	3 cut(s)	383, 1946, 2169
BciVI	GTATCC	3 cut(s)	804, 920, 1945
BcnI	CCSGG	2 cut(s)	1494, 2271
BcoDI	GTCTC	2 cut(s)	1516, 2381
BfaI	CTAG	3 cut(s)	1046, 1067, 2189
BfmI	CTRYAG	2 cut(s)	364, 422
BfuI	GTATCC	3 cut(s)	804, 920, 1945
BglI	GCCNNNNNGGC	1 cut(s)	1134
BglII	AGATCT	1 cut(s)	1016
BisI	GCNGC	4 cut(s)	128, 504, 735, 2056
BlnI	CCTAGG	1 cut(s)	1066
BlsI	GCNGC	4 cut(s)	129, 505, 736, 2057
Bme1390I	CCNGG	5 cut(s)	383, 1494, 1946, 2169, 2271
Bme18I	GGWCC	4 cut(s)	352, 718, 2444, 2471
BmeRI	GACNNNNNGTC	1 cut(s)	2497
BmeT110I	CYCGRG	1 cut(s)	101
BmgT120I	GGNCC	7 cut(s)	352, 495, 496, 718, 2230, 2444, 2471
BmrFI	CCNGG	5 cut(s)	383, 1494, 1946, 2169, 2271
BmrI	ACTGGG	1 cut(s)	766
BmuI	ACTGGG	1 cut(s)	766
BpiI	GAAGAC	2 cut(s)	1038, 2076
BpmI	CTGGAG	1 cut(s)	1298
Bpu10I	CCTNAGC	1 cut(s)	507
BpuEI	CTTGAG	3 cut(s)	340, 420, 1285
BpuMI	CCSGG	2 cut(s)	1494, 2271
Bsa29I	ATCGAT	1 cut(s)	327
BsaBI	GATNNNNATC	1 cut(s)	1956
BsaHI	GRCGYC	1 cut(s)	1933
BsaWI	WCCGGW	1 cut(s)	651
BsaXI	ACNNNNNCTCC	6 cut(s)	34, 64, 657, 663, 687, 693
Bse118I	RCCGGY	2 cut(s)	1539, 2326
Bse1I	ACTGG	5 cut(s)	25, 761, 937, 1936, 2446
Bse3DI	GCAATG	1 cut(s)	1554
Bse8I	GATNNNNATC	1 cut(s)	1956
BseBI	CCWGG	3 cut(s)	383, 1946, 2169
BseCI	ATCGAT	1 cut(s)	327
BseJI	GATNNNNATC	1 cut(s)	1956
BseMI	GCAATG	1 cut(s)	1554
BseMII	CTCAG	4 cut(s)	127, 521, 870, 1641
BseNI	ACTGG	5 cut(s)	25, 761, 937, 1936, 2446
BseRI	GAGGAG	4 cut(s)	42, 1295, 2384, 2399
BseSI	GKGCMC	1 cut(s)	499
BseXI	GCAGC	3 cut(s)	139, 746, 2067
BseYI	CCCAGC	1 cut(s)	721
BsgI	GTGCAG	1 cut(s)	1948
Bsh1236I	CGCG	2 cut(s)	907, 2094
BshFI	GGCC	8 cut(s)	497, 947, 1128, 1507, 1553, 1872, 2217, 2232
BshNI	GGYRCC	2 cut(s)	13, 598
BshVI	ATCGAT	1 cut(s)	327
BsiHKAI	GWGCWC	2 cut(s)	77, 2482
BsiHKCI	CYCGRG	1 cut(s)	101
BsiSI	CCGG	5 cut(s)	652, 1494, 1540, 2270, 2327
BsiWI	CGTACG	1 cut(s)	408
BslFI	GGGAC	3 cut(s)	542, 2286, 2430
BsmAI	GTCTC	2 cut(s)	1516, 2381
BsmFI	GGGAC	3 cut(s)	542, 2286, 2430
BsmI	GAATGC	3 cut(s)	958, 1712, 1987
BsnI	GGCC	8 cut(s)	497, 947, 1128, 1507, 1553, 1872, 2217, 2232
BsoBI	CYCGRG	1 cut(s)	101
Bsp120I	GGGCCC	1 cut(s)	495
Bsp1286I	GDGCHC	3 cut(s)	77, 499, 2482
Bsp143I	GATC	8 cut(s)	328, 385, 682, 760, 1016, 1437, 1681, 1957
Bsp19I	CCATGG	1 cut(s)	2218
BspACI	CCGC	8 cut(s)	139, 504, 865, 905, 1075, 1246, 2094, 2381
BspANI	GGCC	8 cut(s)	497, 947, 1128, 1507, 1553, 1872, 2217, 2232
BspCNI	CTCAG	4 cut(s)	126, 520, 869, 1642
BspDI	ATCGAT	1 cut(s)	327
BspFNI	CGCG	2 cut(s)	907, 2094
BspHI	TCATGA	1 cut(s)	1372
BspPI	GGATC	4 cut(s)	393, 755, 768, 1432
BspT107I	GGYRCC	2 cut(s)	13, 598
BsrBI	CCGCTC	1 cut(s)	506
BsrDI	GCAATG	1 cut(s)	1554
BsrFI	RCCGGY	2 cut(s)	1539, 2326
BsrI	ACTGG	5 cut(s)	25, 761, 937, 1936, 2446
BssAI	RCCGGY	2 cut(s)	1539, 2326
BssMI	GATC	8 cut(s)	328, 385, 682, 760, 1016, 1437, 1681, 1957
BssNI	GRCGYC	1 cut(s)	1933
BssSI	CACGAG	1 cut(s)	822
BssT1I	CCWWGG	7 cut(s)	225, 295, 310, 813, 1066, 2218, 2494
Bst2BI	CACGAG	1 cut(s)	822
Bst2UI	CCWGG	3 cut(s)	383, 1946, 2169
Bst4CI	ACNGT	5 cut(s)	488, 715, 934, 1465, 2300
Bst6I	CTCTTC	3 cut(s)	1593, 2218, 2394
BstACI	GRCGYC	1 cut(s)	1933
BstAPI	GCANNNNNTGC	2 cut(s)	136, 731
BstC8I	GCNNGC	3 cut(s)	132, 1425, 1545
BstDEI	CTNAG	4 cut(s)	113, 507, 856, 1650
BstDSI	CCRYGG	3 cut(s)	714, 1339, 2218
BstFNI	CGCG	2 cut(s)	907, 2094
BstHHI	GCGC	2 cut(s)	1795, 2094
BstKTI	GATC	8 cut(s)	331, 388, 685, 763, 1019, 1440, 1684, 1960
BstMAI	GTCTC	2 cut(s)	1516, 2381
BstMBI	GATC	8 cut(s)	328, 385, 682, 760, 1016, 1437, 1681, 1957
BstMWI	GCNNNNNNNGC	6 cut(s)	136, 306, 503, 512, 731, 1134
BstNI	CCWGG	3 cut(s)	383, 1946, 2169
BstNSI	RCATGY	3 cut(s)	842, 1750, 2340
BstSCI	CCNGG	5 cut(s)	381, 1492, 1944, 2167, 2269
BstSFI	CTRYAG	2 cut(s)	364, 422
BstSLI	GKGCMC	1 cut(s)	499
BstUI	CGCG	2 cut(s)	907, 2094
BstV1I	GCAGC	3 cut(s)	139, 746, 2067
BstV2I	GAAGAC	2 cut(s)	1038, 2076
BstX2I	RGATCY	2 cut(s)	760, 1016
BstYI	RGATCY	2 cut(s)	760, 1016
Bsu15I	ATCGAT	1 cut(s)	327
BsuI	GTATCC	3 cut(s)	804, 920, 1945
BsuRI	GGCC	8 cut(s)	497, 947, 1128, 1507, 1553, 1872, 2217, 2232
BsuTUI	ATCGAT	1 cut(s)	327
BtgI	CCRYGG	3 cut(s)	714, 1339, 2218
BtgZI	GCGATG	1 cut(s)	159
BtsI	GCAGTG	1 cut(s)	141
BtsIMutI	CAGTG	3 cut(s)	141, 1647, 1943
Cac8I	GCNNGC	3 cut(s)	132, 1425, 1545
CciI	TCATGA	1 cut(s)	1372
CfoI	GCGC	2 cut(s)	1795, 2094
Cfr10I	RCCGGY	2 cut(s)	1539, 2326
Cfr13I	GGNCC	7 cut(s)	352, 495, 496, 718, 2230, 2444, 2471
ClaI	ATCGAT	1 cut(s)	327
CseI	GACGC	1 cut(s)	1941
CsiI	ACCWGGT	1 cut(s)	1944
Csp6I	GTAC	6 cut(s)	88, 409, 484, 599, 1645, 2176
CviQI	GTAC	6 cut(s)	88, 409, 484, 599, 1645, 2176
DdeI	CTNAG	4 cut(s)	113, 507, 856, 1650
DpnI	GATC	8 cut(s)	330, 387, 684, 762, 1018, 1439, 1683, 1959
DpnII	GATC	8 cut(s)	328, 385, 682, 760, 1016, 1437, 1681, 1957
DraI	TTTAAA	1 cut(s)	1288
DriI	GACNNNNNGTC	1 cut(s)	2497
EaeI	YGGCCR	4 cut(s)	945, 1551, 1870, 2215
Eam1104I	CTCTTC	3 cut(s)	1593, 2218, 2394
Eam1105I	GACNNNNNGTC	1 cut(s)	2497
EarI	CTCTTC	3 cut(s)	1593, 2218, 2394
Eco130I	CCWWGG	7 cut(s)	225, 295, 310, 813, 1066, 2218, 2494
Eco24I	GRGCYC	1 cut(s)	499
Eco47I	GGWCC	4 cut(s)	352, 718, 2444, 2471
Eco57I	CTGAAG	2 cut(s)	1050, 1536
Eco88I	CYCGRG	1 cut(s)	101
EcoO109I	RGGNCCY	1 cut(s)	496
EcoRII	CCWGG	3 cut(s)	381, 1944, 2167
EcoT14I	CCWWGG	7 cut(s)	225, 295, 310, 813, 1066, 2218, 2494
EcoT22I	ATGCAT	1 cut(s)	973
EcoT38I	GRGCYC	1 cut(s)	499
ErhI	CCWWGG	7 cut(s)	225, 295, 310, 813, 1066, 2218, 2494
FalI	AAGNNNNNCTT	2 cut(s)	341, 373
FaqI	GGGAC	3 cut(s)	542, 2286, 2430
FauI	CCCGC	3 cut(s)	872, 1068, 2087
FblI	GTMKAC	3 cut(s)	421, 753, 2241
Fnu4HI	GCNGC	4 cut(s)	128, 504, 735, 2056
FriOI	GRGCYC	1 cut(s)	499
Fsp4HI	GCNGC	4 cut(s)	128, 504, 735, 2056
FspBI	CTAG	3 cut(s)	1046, 1067, 2189
GlaI	GCGC	2 cut(s)	1794, 2093
GluI	GCNGC	4 cut(s)	128, 504, 735, 2056
GsaI	CCCAGC	1 cut(s)	725
GsuI	CTGGAG	1 cut(s)	1298
HaeIII	GGCC	8 cut(s)	497, 947, 1128, 1507, 1553, 1872, 2217, 2232
HapII	CCGG	5 cut(s)	652, 1494, 1540, 2270, 2327
HgaI	GACGC	1 cut(s)	1941
HhaI	GCGC	2 cut(s)	1795, 2094
Hin1I	GRCGYC	1 cut(s)	1933
Hin6I	GCGC	2 cut(s)	1793, 2092
HinP1I	GCGC	2 cut(s)	1793, 2092
HincII	GTYRAC	2 cut(s)	1051, 2037
HindII	GTYRAC	2 cut(s)	1051, 2037
HinfI	GANTC	7 cut(s)	99, 111, 315, 369, 605, 700, 1163
HpaII	CCGG	5 cut(s)	652, 1494, 1540, 2270, 2327
HphI	GGTGA	3 cut(s)	1328, 1709, 2316
Hpy166II	GTNNAC	9 cut(s)	422, 484, 718, 754, 1051, 1096, 1647, 2037, 2242
Hpy188I	TCNGA	7 cut(s)	155, 390, 775, 859, 1437, 1516, 2509
Hpy188III	TCNNGA	9 cut(s)	96, 319, 509, 1020, 1373, 1679, 1816, 1877, 2279
Hpy8I	GTNNAC	9 cut(s)	422, 484, 718, 754, 1051, 1096, 1647, 2037, 2242
Hpy99I	CGWCG	2 cut(s)	86, 2509
HpyAV	CCTTC	9 cut(s)	387, 407, 464, 1511, 1606, 1895, 2135, 2255, 2320
HpyCH4III	ACNGT	5 cut(s)	488, 715, 934, 1465, 2300
HpyCH4IV	ACGT	2 cut(s)	407, 897
HpyCH4V	TGCA	8 cut(s)	340, 734, 971, 1106, 1460, 1712, 1929, 2186
HpyF10VI	GCNNNNNNNGC	6 cut(s)	136, 306, 503, 512, 731, 1134
HpyF3I	CTNAG	4 cut(s)	113, 507, 856, 1650
HpySE526I	ACGT	2 cut(s)	407, 897
Hsp92I	GRCGYC	1 cut(s)	1933
HspAI	GCGC	2 cut(s)	1793, 2092
KpnI	GGTACC	1 cut(s)	602
Kzo9I	GATC	8 cut(s)	328, 385, 682, 760, 1016, 1437, 1681, 1957
LmnI	GCTCC	3 cut(s)	554, 823, 955
Lsp1109I	GCAGC	3 cut(s)	139, 746, 2067
MabI	ACCWGGT	1 cut(s)	1944
MaeI	CTAG	3 cut(s)	1046, 1067, 2189
MaeII	ACGT	2 cut(s)	407, 897
MaeIII	GTNAC	8 cut(s)	988, 1109, 1315, 1592, 1676, 1697, 2159, 2489
MalI	GATC	8 cut(s)	330, 387, 684, 762, 1018, 1439, 1683, 1959
MbiI	CCGCTC	1 cut(s)	506
MboI	GATC	8 cut(s)	328, 385, 682, 760, 1016, 1437, 1681, 1957
MfeI	CAATTG	2 cut(s)	1689, 1707
MflI	RGATCY	2 cut(s)	760, 1016
MhlI	GDGCHC	3 cut(s)	77, 499, 2482
MlsI	TGGCCA	3 cut(s)	1553, 1872, 2217
MluNI	TGGCCA	3 cut(s)	1553, 1872, 2217
MlyI	GAGTC	3 cut(s)	93, 105, 1172
MmeI	TCCRAC	3 cut(s)	823, 1284, 2487
Mox20I	TGGCCA	3 cut(s)	1553, 1872, 2217
Mph1103I	ATGCAT	1 cut(s)	973
MroXI	GAANNNNTTC	1 cut(s)	780
MscI	TGGCCA	3 cut(s)	1553, 1872, 2217
MseI	TTAA	5 cut(s)	459, 534, 645, 1038, 1287
MslI	CAYNNNNRTG	2 cut(s)	666, 1101
Msp20I	TGGCCA	3 cut(s)	1553, 1872, 2217
MspI	CCGG	5 cut(s)	652, 1494, 1540, 2270, 2327
MspR9I	CCNGG	5 cut(s)	383, 1494, 1946, 2169, 2271
MunI	CAATTG	2 cut(s)	1689, 1707
Mva1269I	GAATGC	3 cut(s)	958, 1712, 1987
MvaI	CCWGG	3 cut(s)	383, 1946, 2169
MvnI	CGCG	2 cut(s)	907, 2094
MwoI	GCNNNNNNNGC	6 cut(s)	136, 306, 503, 512, 731, 1134
NciI	CCSGG	2 cut(s)	1494, 2271
NcoI	CCATGG	1 cut(s)	2218
NdeII	GATC	8 cut(s)	328, 385, 682, 760, 1016, 1437, 1681, 1957
NmuCI	GTSAC	6 cut(s)	988, 1592, 1676, 1697, 2159, 2489
NsiI	ATGCAT	1 cut(s)	973
NspI	RCATGY	3 cut(s)	842, 1750, 2340
PagI	TCATGA	1 cut(s)	1372
PciI	ACATGT	2 cut(s)	838, 2336
PctI	GAATGC	3 cut(s)	958, 1712, 1987
PdmI	GAANNNNTTC	1 cut(s)	780
PfeI	GAWTC	4 cut(s)	315, 369, 605, 700
Pfl23II	CGTACG	1 cut(s)	408
PflFI	GACNNNGTC	1 cut(s)	1500
PflMI	CCANNNNNTGG	2 cut(s)	301, 943
PkrI	GCNGC	4 cut(s)	129, 505, 736, 2057
PleI	GAGTC	3 cut(s)	93, 105, 1171
PpsI	GAGTC	3 cut(s)	93, 105, 1171
PscI	ACATGT	2 cut(s)	838, 2336
Psp6I	CCWGG	3 cut(s)	381, 1944, 2167
PspFI	CCCAGC	1 cut(s)	721
PspGI	CCWGG	3 cut(s)	381, 1944, 2167
PspLI	CGTACG	1 cut(s)	408
PspOMI	GGGCCC	1 cut(s)	495
PspPI	GGNCC	7 cut(s)	352, 495, 496, 718, 2230, 2444, 2471
PsuI	RGATCY	2 cut(s)	760, 1016
PsyI	GACNNNGTC	1 cut(s)	1500
RsaI	GTAC	6 cut(s)	89, 410, 485, 600, 1646, 2177
RsaNI	GTAC	6 cut(s)	88, 409, 484, 599, 1645, 2176
RseI	CAYNNNNRTG	2 cut(s)	666, 1101
SaqAI	TTAA	5 cut(s)	459, 534, 645, 1038, 1287
SatI	GCNGC	4 cut(s)	128, 504, 735, 2056
Sau3AI	GATC	8 cut(s)	328, 385, 682, 760, 1016, 1437, 1681, 1957
Sau96I	GGNCC	7 cut(s)	352, 495, 496, 718, 2230, 2444, 2471
SchI	GAGTC	3 cut(s)	93, 105, 1172
ScrFI	CCNGG	5 cut(s)	383, 1494, 1946, 2169, 2271
SduI	GDGCHC	3 cut(s)	77, 499, 2482
SexAI	ACCWGGT	1 cut(s)	1944
SfcI	CTRYAG	2 cut(s)	364, 422
SinI	GGWCC	4 cut(s)	352, 718, 2444, 2471
SmiMI	CAYNNNNRTG	2 cut(s)	666, 1101
SmlI	CTYRAG	3 cut(s)	319, 435, 1264
SmoI	CTYRAG	3 cut(s)	319, 435, 1264
SsiI	CCGC	8 cut(s)	139, 504, 865, 905, 1075, 1246, 2094, 2381
SspMI	CTAG	3 cut(s)	1046, 1067, 2189
StyD4I	CCNGG	5 cut(s)	381, 1492, 1944, 2167, 2269
StyI	CCWWGG	7 cut(s)	225, 295, 310, 813, 1066, 2218, 2494
TaaI	ACNGT	5 cut(s)	488, 715, 934, 1465, 2300
TaiI	ACGT	2 cut(s)	410, 900
TaqI	TCGA	3 cut(s)	327, 1303, 2353
TatI	WGTACW	1 cut(s)	2175
TauI	GCSGC	1 cut(s)	506
TfiI	GAWTC	4 cut(s)	315, 369, 605, 700
Tru1I	TTAA	5 cut(s)	459, 534, 645, 1038, 1287
Tru9I	TTAA	5 cut(s)	459, 534, 645, 1038, 1287
TscAI	CASTG	3 cut(s)	141, 1654, 1943
TseFI	GTSAC	6 cut(s)	988, 1592, 1676, 1697, 2159, 2489
TseI	GCWGC	3 cut(s)	127, 734, 2055
Tsp45I	GTSAC	6 cut(s)	988, 1592, 1676, 1697, 2159, 2489
TspRI	CASTG	3 cut(s)	141, 1654, 1943
Tth111I	GACNNNGTC	1 cut(s)	1500
Van91I	CCANNNNNTGG	2 cut(s)	301, 943
VpaK11BI	GGWCC	4 cut(s)	352, 718, 2444, 2471
XapI	RAATTY	9 cut(s)	428, 449, 616, 647, 884, 1289, 1617, 2002, 2287
XceI	RCATGY	3 cut(s)	842, 1750, 2340
XcmI	CCANNNNNNNNNTGG	1 cut(s)	559
XmaJI	CCTAGG	1 cut(s)	1066
XmiI	GTMKAC	3 cut(s)	421, 753, 2241
XmnI	GAANNNNTTC	1 cut(s)	780
XspI	CTAG	3 cut(s)	1046, 1067, 2189
Zsp2I	ATGCAT	1 cut(s)	973

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.