Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6A

Orthogroup ID

OG0013627

Physical Location & Seq

Genomic Coordinates Reverse (-)

63013571 .. 63019204

Gene Structure 5634 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6AG443300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2196 bp

ATGTTGGGTTCTTTGGGTCTTCATCTCACTTGTTCTTTTGAGTCTTTATCGTGTTCCAAGGACTACCTGAGTCCGAGGGTAACTCAAACTAAAGCAGTCTCTTTTCAGCTTCGACATGGAGTATTGTCCCCTCCAGAAGGTTTAAGATGTGGGAAGTTGGAGCGTAGTCCCAGAGTTTCAGCAGTTTTGACGGACGACAGCTCGAGAATGACAGTCAGTGATGAAGAGATGGAAGATATTGGTGTTCTGAGAACAGATTCAAACTTGGAGCCATACAAAGATCACTTCAAGTATCGAATAAAAAGATATTTGGAGCAGAAGAGTCTCTTTGAGAAACATGAAGGAGGTTTGGAGGAATTCGCGCAAGGTTATGCGAAATTCGGATTTAACAGAGAAGATGGTGGAATTGTGTACCGTGAATGGGCCCCTGCAGCTCAGGAGGCACAACTTGTTGGAGACTTTAATGGATGGGATGGCAGCAAATACGAGATGGAGAAGGACCAATTTGGGGTATGGAGTATTAAGATACCCGATTTTGGTGGAAGTCCAGCTATTCCTCACAATTCAAAGGTTAAATTCCGGTTCAAGCATGGAAAAGGAGTTTGGGTTGATCGTATTCCTGCTTGGATTAAATATGCCACTGTGGACCCAACAAGATTTGCAGCTCCCTATGATGGTGTGTACTGGGATCCACCACCATCAGAAAGGTATCAGTTTAAGTACGGTCGTCCTCCAAAACCCAAGGCTCCACGAATATATGAGGCCCATGTTGGAATGAGTAGCTCAGAACCCCGCATCAGTTCATACAGAGAATTTGCTGATAATGTTTTGCCTCGTATCCAAGCAAATAACTATAACACAGTCCAGCTGATGGCCGTGATGGAGCATTCCTATTATGCATCCTTCGGTTATCATGTGACAAACTTCTTTGCCGTAAGCAGTAGATCTGGAACACCTGAGGACCTTAAATATCTAATTGATAAAGCACATTCCCTAGGTTTGCGTGTATTGATGGATGTTGTTCACAGTCATGCAAGTAACAACGTCACTGATGGCCTCAGTGGCTTTGAAGTTGGTCAATGCACACAAGAGTCCTATTTCCACACAGGAGATAGAGGCTACCATAAGCTATGGGATAGCCGACTTTTCAACTATGCTAATTGGGAAGTTCTCCGCTTCTTTTTATCCAACTTAAGGTGGTGGCTGGAGGAGTATCAATTTGATGGGTTTCGATTTGATGGAGTAACTTCCATGCTGTATCATCACCATGGCATAAATATGGCATTTACAGGGAATTATGATGAGTACTTCAGTGAGGCAACAGATGTTGATGCTGTTGTTTATTTGATGCTTGCCAACCATCTGATCCATAAAATTTTGCCATCTGCAACTGTAATTGCTGAAGATGTTTCTGGGATGCCTGGACTTGGTCGGCCTGTCTCTGAAGGAGGTGTTGGTTTTGAGTACCGGCTGGCAATGGCCATCCCTGACAAGTGGATTGATTATGTGAAAAACAAGAAAGATGAAGAGTGGTCGATGAAGGAAATTTCATGGAGCTTGACGAATAGGAGATACACCGAGAAGTGTGTTTCCTATGCTGAGAGTCATGACCAGGCAATTGTGGGTGACAAGACGATTGCATTCTTTTTGATGGATAAAGAAATGTACTCTGGCATGTCTTGTTTGAATGAAGCTTCTCCTACTGTTGAGCGCGGGGTTGCTCTTCACAAGATGATACATTTTATAACTATGGCATTAGGAGGCGAGGGTTACCTTAACTTTATGGGTAATGAGTTTGGACACCCTGAGTGGATCGACTTTCCAAGAGAAGGCAATGGGTGGAGTTATGAAAAGTGCAGACGCCAATGGAACCTGGTGGATACAGATCATTTAAGATATAAGTTTATGAACGCATTTGACAGAGCTATGCACTTGCTTGATGAGAAATTTTCGTTCCTTTCATCAGCGAAGCAGATAGTGAGCAGCACAAATGAAGAAGACAAGGTTATTGTCTTTGAGCGTGGAGATCTAGTTTTTGTATTTAATTTTCATCCAGTAAACACATACGATGGGTACAAAGTGGGGTGTGACTTACCTGGGAAGTACAGAGTTACACTAGATAGTGATGCTTTTGAATTCGGTGGACATGGAAGAGTAAGGATTCCCTTTACATACCTATTCCCAGTGTTGTATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003844 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0006073 GO:0008150 GO:0008152 GO:0009501 GO:0009507 GO:0009536 GO:0009987 GO:0016740 GO:0016757 GO:0016758 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase Alpha-amylase_C CBM_48

Protein Analysis

731

Amino Acids

83.95

Weight (kDa)

5.95

Isoelectric Point (pI)

34.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_48	PF02922	126 - 209	1.8e-20	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Alpha-amylase	PF00128	299 - 349	5.4e-09	Alpha amylase, catalytic domain
Alpha-amylase_C	PF02806	661 - 716	3.1e-15	Alpha amylase, C-terminal all-beta domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013627)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g37381 FvH4_2g37410
malus_domestica	MD08G1002300.v1.1
prunus_persica	Prupe.1G354000_v2.0.a1 Prupe.1G354000_v2.0.a1 Prupe.1G354000_v2.0.a1
pyrus_communis	pycom08g00150
rosa_chinensis	RchiOBHm_Chr6g0304311
rosa_laevigata	RLG00000010992
rosa_multiflora	Rmu_ssc0000218.1_g000037
rosa_roxburghii	Rroxscaffold_7G00163850
rosa_rugosa	Rorug06G0329900 Rorug06G0330000
rosa_samantha	Rh6AG443300 Rh6BG459100 Rh6CG456100 Rh6DG442800
rosa_wichuraiana	Rw6G038470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1745
AccB7I	CCANNNNNTGG	1 cut(s)	871
AccII	CGCG	2 cut(s)	362, 1713
AciI	CCGC	3 cut(s)	793, 1174, 1713
AclWI	GGATC	4 cut(s)	683, 696, 1360, 1820
AcoI	YGGCCR	2 cut(s)	873, 1479
AcsI	RAATTY	8 cut(s)	356, 377, 575, 812, 1374, 1545, 1946, 2135
AcuI	CTGAAG	3 cut(s)	1294, 1422, 1464
AcyI	GRCGYC	1 cut(s)	1861
AfaI	GTAC	8 cut(s)	413, 683, 722, 1307, 1466, 1667, 2075, 2105
AfiI	CCNNNNNNNGG	9 cut(s)	137, 421, 508, 536, 674, 871, 1194, 1427, 1829
AflII	CTTAAG	1 cut(s)	1192
AgsI	TTSAA	8 cut(s)	261, 289, 567, 586, 1070, 1150, 1687, 2135
AjnI	CCWGG	4 cut(s)	1420, 1611, 1872, 2095
AjuI	GAANNNNNNNTTGG	4 cut(s)	586, 618, 1437, 1469
Alw26I	GTCTC	4 cut(s)	103, 329, 450, 1444
AlwI	GGATC	4 cut(s)	683, 696, 1360, 1820
Ama87I	CYCGRG	1 cut(s)	202
AoxI	GGCC	6 cut(s)	423, 762, 873, 1054, 1433, 1479
ApaI	GGGCCC	1 cut(s)	427
ApeKI	GCWGC	4 cut(s)	431, 477, 662, 1983
ApoI	RAATTY	8 cut(s)	356, 377, 575, 812, 1374, 1545, 1946, 2135
Asp700I	GAANNNNTTC	1 cut(s)	256
AspA2I	CCTAGG	1 cut(s)	994
AspLEI	GCGC	2 cut(s)	364, 1713
AspS9I	GGNCC	6 cut(s)	423, 424, 499, 646, 763, 961
AsuHPI	GGTGA	2 cut(s)	1256, 1637
AvaI	CYCGRG	1 cut(s)	202
AvaII	GGWCC	3 cut(s)	499, 646, 961
AvrII	CCTAGG	1 cut(s)	994
AxyI	CCTNAGG	1 cut(s)	957
BaeGI	GKGCMC	1 cut(s)	427
BalI	TGGCCA	1 cut(s)	1481
BamHI	GGATCC	1 cut(s)	688
BanII	GRGCYC	1 cut(s)	427
BbsI	GAAGAC	2 cut(s)	11, 2004
BbvI	GCAGC	4 cut(s)	443, 489, 674, 1995
BceAI	ACGGC	2 cut(s)	860, 917
BciT130I	CCWGG	4 cut(s)	1422, 1613, 1874, 2097
BciVI	GTATCC	2 cut(s)	848, 1873
BcoDI	GTCTC	4 cut(s)	103, 329, 450, 1444
BfaI	CTAG	3 cut(s)	995, 2030, 2117
BfmI	CTRYAG	1 cut(s)	429
BfrI	CTTAAG	1 cut(s)	1192
BfuI	GTATCC	2 cut(s)	848, 1873
BglI	GCCNNNNNGGC	1 cut(s)	1062
BglII	AGATCT	2 cut(s)	944, 2026
BisI	GCNGC	4 cut(s)	432, 478, 663, 1984
BlnI	CCTAGG	1 cut(s)	994
BlsI	GCNGC	4 cut(s)	433, 479, 664, 1985
BmcAI	AGTACT	1 cut(s)	1307
Bme1390I	CCNGG	4 cut(s)	1422, 1613, 1874, 2097
Bme18I	GGWCC	3 cut(s)	499, 646, 961
BmeT110I	CYCGRG	1 cut(s)	202
BmgT120I	GGNCC	6 cut(s)	423, 424, 499, 646, 763, 961
BmiI	GGNNCC	7 cut(s)	270, 425, 426, 648, 690, 747, 1871
BmrFI	CCNGG	4 cut(s)	1422, 1613, 1874, 2097
BmrI	ACTGGG	2 cut(s)	694, 2177
BmsI	GCATC	6 cut(s)	804, 908, 1321, 1338, 1407, 2116
BmuI	ACTGGG	2 cut(s)	694, 2177
BpiI	GAAGAC	2 cut(s)	11, 2004
BplI	GAGNNNNNCTC	2 cut(s)	67, 99
BpmI	CTGGAG	2 cut(s)	117, 1226
Bpu10I	CCTNAGC	1 cut(s)	435
BsaBI	GATNNNNATC	1 cut(s)	1884
BsaHI	GRCGYC	1 cut(s)	1861
BsaJI	CCNNGG	6 cut(s)	57, 74, 741, 994, 1267, 2096
BsaWI	WCCGGW	1 cut(s)	579
BsaXI	ACNNNNNCTCC	4 cut(s)	591, 621, 2016, 2046
Bsc4I	CCNNNNNNNGG	9 cut(s)	137, 421, 508, 536, 674, 871, 1194, 1427, 1829
Bse118I	RCCGGY	1 cut(s)	1467
Bse1I	ACTGG	3 cut(s)	689, 2054, 2183
Bse21I	CCTNAGG	1 cut(s)	957
Bse3DI	GCAATG	2 cut(s)	1482, 1840
Bse8I	GATNNNNATC	1 cut(s)	1884
BseBI	CCWGG	4 cut(s)	1422, 1613, 1874, 2097
BseDI	CCNNGG	6 cut(s)	57, 74, 741, 994, 1267, 2096
BseGI	GGATG	7 cut(s)	473, 478, 899, 1021, 1422, 1482, 2050
BseJI	GATNNNNATC	1 cut(s)	1884
BseLI	CCNNNNNNNGG	9 cut(s)	137, 421, 508, 536, 674, 871, 1194, 1427, 1829
BseMI	GCAATG	2 cut(s)	1482, 1840
BseMII	CTCAG	8 cut(s)	59, 239, 449, 798, 948, 1072, 1590, 1797
BseNI	ACTGG	3 cut(s)	689, 2054, 2183
BseRI	GAGGAG	1 cut(s)	1223
BseSI	GKGCMC	1 cut(s)	427
BseXI	GCAGC	4 cut(s)	443, 489, 674, 1995
BsgI	GTGCAG	1 cut(s)	1876
Bsh1236I	CGCG	2 cut(s)	362, 1713
Bsh1285I	CGRYCG	1 cut(s)	727
BshFI	GGCC	6 cut(s)	425, 764, 875, 1056, 1435, 1481
BsiEI	CGRYCG	1 cut(s)	727
BsiHKCI	CYCGRG	1 cut(s)	202
BsiSI	CCGG	2 cut(s)	580, 1468
BslFI	GGGAC	2 cut(s)	112, 153
BslI	CCNNNNNNNGG	9 cut(s)	137, 421, 508, 536, 674, 871, 1194, 1427, 1829
BsmAI	GTCTC	4 cut(s)	103, 329, 450, 1444
BsmFI	GGGAC	2 cut(s)	112, 153
BsmI	GAATGC	2 cut(s)	886, 1640
BsnI	GGCC	6 cut(s)	425, 764, 875, 1056, 1435, 1481
BsoBI	CYCGRG	1 cut(s)	202
Bsp120I	GGGCCC	1 cut(s)	423
Bsp1286I	GDGCHC	1 cut(s)	427
Bsp143I	GATC	8 cut(s)	280, 610, 688, 944, 1365, 1812, 1885, 2026
Bsp19I	CCATGG	1 cut(s)	1267
BspACI	CCGC	3 cut(s)	793, 1174, 1713
BspANI	GGCC	6 cut(s)	425, 764, 875, 1056, 1435, 1481
BspCNI	CTCAG	8 cut(s)	60, 240, 448, 797, 949, 1071, 1591, 1798
BspFNI	CGCG	2 cut(s)	362, 1713
BspHI	TCATGA	1 cut(s)	1606
BspLI	GGNNCC	7 cut(s)	270, 425, 426, 648, 690, 747, 1871
BspMAI	CTGCAG	1 cut(s)	433
BspPI	GGATC	4 cut(s)	683, 696, 1360, 1820
BspQI	GCTCTTC	1 cut(s)	1728
BspTI	CTTAAG	1 cut(s)	1192
BsrDI	GCAATG	2 cut(s)	1482, 1840
BsrFI	RCCGGY	1 cut(s)	1467
BsrI	ACTGG	3 cut(s)	689, 2054, 2183
BssAI	RCCGGY	1 cut(s)	1467
BssECI	CCNNGG	6 cut(s)	57, 74, 741, 994, 1267, 2096
BssMI	GATC	8 cut(s)	280, 610, 688, 944, 1365, 1812, 1885, 2026
BssNI	GRCGYC	1 cut(s)	1861
BssT1I	CCWWGG	4 cut(s)	57, 741, 994, 1267
Bst2UI	CCWGG	4 cut(s)	1422, 1613, 1874, 2097
Bst4CI	ACNGT	8 cut(s)	214, 416, 643, 725, 862, 1028, 1393, 1705
Bst6I	CTCTTC	5 cut(s)	219, 314, 1521, 1728, 2146
BstACI	GRCGYC	1 cut(s)	1861
BstAFI	CTTAAG	1 cut(s)	1192
BstC8I	GCNNGC	2 cut(s)	1353, 1473
BstDEI	CTNAG	8 cut(s)	68, 248, 435, 784, 957, 1058, 1599, 1806
BstDSI	CCRYGG	1 cut(s)	1267
BstEII	GGTNACC	1 cut(s)	1769
BstENI	CCTNNNNNAGG	1 cut(s)	135
BstF5I	GGATG	7 cut(s)	473, 478, 899, 1021, 1422, 1482, 2050
BstFNI	CGCG	2 cut(s)	362, 1713
BstHHI	GCGC	2 cut(s)	364, 1713
BstKTI	GATC	8 cut(s)	283, 613, 691, 947, 1368, 1815, 1888, 2029
BstMAI	GTCTC	4 cut(s)	103, 329, 450, 1444
BstMBI	GATC	8 cut(s)	280, 610, 688, 944, 1365, 1812, 1885, 2026
BstMCI	CGRYCG	1 cut(s)	727
BstMWI	GCNNNNNNNGC	3 cut(s)	431, 440, 1062
BstNI	CCWGG	4 cut(s)	1422, 1613, 1874, 2097
BstNSI	RCATGY	1 cut(s)	1678
BstPI	GGTNACC	1 cut(s)	1769
BstSCI	CCNGG	4 cut(s)	1420, 1611, 1872, 2095
BstSFI	CTRYAG	1 cut(s)	429
BstSLI	GKGCMC	1 cut(s)	427
BstUI	CGCG	2 cut(s)	362, 1713
BstV1I	GCAGC	4 cut(s)	443, 489, 674, 1995
BstV2I	GAAGAC	2 cut(s)	11, 2004
BstX2I	RGATCY	3 cut(s)	688, 944, 2026
BstYI	RGATCY	3 cut(s)	688, 944, 2026
Bsu36I	CCTNAGG	1 cut(s)	957
BsuI	GTATCC	2 cut(s)	848, 1873
BsuRI	GGCC	6 cut(s)	425, 764, 875, 1056, 1435, 1481
BtgI	CCRYGG	1 cut(s)	1267
BtsCI	GGATG	7 cut(s)	473, 478, 899, 1021, 1422, 1482, 2050
BtsIMutI	CAGTG	6 cut(s)	223, 639, 1047, 1066, 1318, 2190
Cac8I	GCNNGC	2 cut(s)	1353, 1473
CciI	TCATGA	1 cut(s)	1606
CfoI	GCGC	2 cut(s)	364, 1713
Cfr10I	RCCGGY	1 cut(s)	1467
Cfr13I	GGNCC	6 cut(s)	423, 424, 499, 646, 763, 961
CseI	GACGC	1 cut(s)	1869
CsiI	ACCWGGT	1 cut(s)	1872
Csp6I	GTAC	8 cut(s)	412, 682, 721, 1306, 1465, 1666, 2074, 2104
CviQI	GTAC	8 cut(s)	412, 682, 721, 1306, 1465, 1666, 2074, 2104
DdeI	CTNAG	8 cut(s)	68, 248, 435, 784, 957, 1058, 1599, 1806
DpnI	GATC	8 cut(s)	282, 612, 690, 946, 1367, 1814, 1887, 2028
DpnII	GATC	8 cut(s)	280, 610, 688, 944, 1365, 1812, 1885, 2026
EaeI	YGGCCR	2 cut(s)	873, 1479
Eam1104I	CTCTTC	5 cut(s)	219, 314, 1521, 1728, 2146
EarI	CTCTTC	5 cut(s)	219, 314, 1521, 1728, 2146
Eco130I	CCWWGG	4 cut(s)	57, 741, 994, 1267
Eco24I	GRGCYC	1 cut(s)	427
Eco47I	GGWCC	3 cut(s)	499, 646, 961
Eco57I	CTGAAG	3 cut(s)	1294, 1422, 1464
Eco81I	CCTNAGG	1 cut(s)	957
Eco88I	CYCGRG	1 cut(s)	202
Eco91I	GGTNACC	1 cut(s)	1769
EcoNI	CCTNNNNNAGG	1 cut(s)	135
EcoO109I	RGGNCCY	2 cut(s)	424, 961
EcoO65I	GGTNACC	1 cut(s)	1769
EcoRI	GAATTC	2 cut(s)	356, 2135
EcoRII	CCWGG	4 cut(s)	1420, 1611, 1872, 2095
EcoT14I	CCWWGG	4 cut(s)	57, 741, 994, 1267
EcoT22I	ATGCAT	1 cut(s)	901
EcoT38I	GRGCYC	1 cut(s)	427
ErhI	CCWWGG	4 cut(s)	57, 741, 994, 1267
FalI	AAGNNNNNCTT	2 cut(s)	311, 343
FaqI	GGGAC	2 cut(s)	112, 153
FauI	CCCGC	2 cut(s)	800, 1706
Fnu4HI	GCNGC	4 cut(s)	432, 478, 663, 1984
FokI	GGATG	7 cut(s)	480, 485, 886, 1028, 1429, 1469, 2037
FriOI	GRGCYC	1 cut(s)	427
Fsp4HI	GCNGC	4 cut(s)	432, 478, 663, 1984
FspBI	CTAG	3 cut(s)	995, 2030, 2117
GlaI	GCGC	2 cut(s)	363, 1712
GluI	GCNGC	4 cut(s)	432, 478, 663, 1984
GsuI	CTGGAG	2 cut(s)	117, 1226
HaeIII	GGCC	6 cut(s)	425, 764, 875, 1056, 1435, 1481
HapII	CCGG	2 cut(s)	580, 1468
HgaI	GACGC	1 cut(s)	1869
HhaI	GCGC	2 cut(s)	364, 1713
Hin1I	GRCGYC	1 cut(s)	1861
Hin6I	GCGC	2 cut(s)	362, 1711
HinP1I	GCGC	2 cut(s)	362, 1711
HindIII	AAGCTT	1 cut(s)	1692
HinfI	GANTC	7 cut(s)	41, 70, 257, 322, 1091, 1603, 2161
HpaII	CCGG	2 cut(s)	580, 1468
HphI	GGTGA	2 cut(s)	1256, 1637
Hpy166II	GTNNAC	6 cut(s)	412, 646, 682, 1024, 2059, 2144
Hpy188I	TCNGA	7 cut(s)	75, 249, 383, 703, 787, 1365, 1444
Hpy188III	TCNNGA	5 cut(s)	134, 204, 437, 948, 1607
Hpy8I	GTNNAC	6 cut(s)	412, 646, 682, 1024, 2059, 2144
HpyAV	CCTTC	7 cut(s)	131, 335, 490, 913, 1439, 1534, 1823
HpyCH4III	ACNGT	8 cut(s)	214, 416, 643, 725, 862, 1028, 1393, 1705
HpyCH4IV	ACGT	1 cut(s)	1044
HpyCH4V	TGCA	9 cut(s)	431, 662, 899, 1034, 1083, 1388, 1640, 1857, 1930
HpyF10VI	GCNNNNNNNGC	3 cut(s)	431, 440, 1062
HpyF3I	CTNAG	8 cut(s)	68, 248, 435, 784, 957, 1058, 1599, 1806
HpySE526I	ACGT	1 cut(s)	1044
Hsp92I	GRCGYC	1 cut(s)	1861
HspAI	GCGC	2 cut(s)	362, 1711
Kzo9I	GATC	8 cut(s)	280, 610, 688, 944, 1365, 1812, 1885, 2026
LguI	GCTCTTC	1 cut(s)	1728
LmnI	GCTCC	7 cut(s)	160, 268, 313, 670, 751, 883, 1554
Lsp1109I	GCAGC	4 cut(s)	443, 489, 674, 1995
LweI	GCATC	6 cut(s)	804, 908, 1321, 1338, 1407, 2116
MabI	ACCWGGT	1 cut(s)	1872
MaeI	CTAG	3 cut(s)	995, 2030, 2117
MaeII	ACGT	1 cut(s)	1044
MaeIII	GTNAC	9 cut(s)	79, 916, 1037, 1045, 1243, 1625, 1769, 2087, 2110
MalI	GATC	8 cut(s)	282, 612, 690, 946, 1367, 1814, 1887, 2028
MboI	GATC	8 cut(s)	280, 610, 688, 944, 1365, 1812, 1885, 2026
MfeI	CAATTG	1 cut(s)	1617
MflI	RGATCY	3 cut(s)	688, 944, 2026
MhlI	GDGCHC	1 cut(s)	427
MlsI	TGGCCA	1 cut(s)	1481
MluNI	TGGCCA	1 cut(s)	1481
MlyI	GAGTC	5 cut(s)	50, 79, 331, 1100, 1612
MmeI	TCCRAC	4 cut(s)	138, 433, 751, 1212
Mox20I	TGGCCA	1 cut(s)	1481
Mph1103I	ATGCAT	1 cut(s)	901
MroXI	GAANNNNTTC	1 cut(s)	256
MscI	TGGCCA	1 cut(s)	1481
MslI	CAYNNNNRTG	3 cut(s)	1029, 1266, 1277
Msp20I	TGGCCA	1 cut(s)	1481
MspA1I	CMGCKG	1 cut(s)	868
MspCI	CTTAAG	1 cut(s)	1192
MspI	CCGG	2 cut(s)	580, 1468
MspR9I	CCNGG	4 cut(s)	1422, 1613, 1874, 2097
MunI	CAATTG	1 cut(s)	1617
Mva1269I	GAATGC	2 cut(s)	886, 1640
MvaI	CCWGG	4 cut(s)	1422, 1613, 1874, 2097
MvnI	CGCG	2 cut(s)	362, 1713
MwoI	GCNNNNNNNGC	3 cut(s)	431, 440, 1062
NcoI	CCATGG	1 cut(s)	1267
NdeII	GATC	8 cut(s)	280, 610, 688, 944, 1365, 1812, 1885, 2026
NlaIV	GGNNCC	7 cut(s)	270, 425, 426, 648, 690, 747, 1871
NmuCI	GTSAC	4 cut(s)	916, 1045, 1625, 2087
NsiI	ATGCAT	1 cut(s)	901
NspI	RCATGY	1 cut(s)	1678
PaeR7I	CTCGAG	1 cut(s)	202
PagI	TCATGA	1 cut(s)	1606
PciSI	GCTCTTC	1 cut(s)	1728
PctI	GAATGC	2 cut(s)	886, 1640
PdmI	GAANNNNTTC	1 cut(s)	256
PfeI	GAWTC	2 cut(s)	257, 2161
PflFI	GACNNNGTC	1 cut(s)	1428
PflMI	CCANNNNNTGG	1 cut(s)	871
PkrI	GCNGC	4 cut(s)	433, 479, 664, 1985
PleI	GAGTC	5 cut(s)	49, 78, 330, 1099, 1611
PpsI	GAGTC	5 cut(s)	49, 78, 330, 1099, 1611
PpuMI	RGGWCCY	1 cut(s)	961
PsiI	TTATAA	1 cut(s)	1745
Psp5II	RGGWCCY	1 cut(s)	961
Psp6I	CCWGG	4 cut(s)	1420, 1611, 1872, 2095
PspEI	GGTNACC	1 cut(s)	1769
PspGI	CCWGG	4 cut(s)	1420, 1611, 1872, 2095
PspN4I	GGNNCC	7 cut(s)	270, 425, 426, 648, 690, 747, 1871
PspOMI	GGGCCC	1 cut(s)	423
PspPI	GGNCC	6 cut(s)	423, 424, 499, 646, 763, 961
PspPPI	RGGWCCY	1 cut(s)	961
PstI	CTGCAG	1 cut(s)	433
PsuI	RGATCY	3 cut(s)	688, 944, 2026
PsyI	GACNNNGTC	1 cut(s)	1428
PvuII	CAGCTG	1 cut(s)	868
RsaI	GTAC	8 cut(s)	413, 683, 722, 1307, 1466, 1667, 2075, 2105
RsaNI	GTAC	8 cut(s)	412, 682, 721, 1306, 1465, 1666, 2074, 2104
RseI	CAYNNNNRTG	3 cut(s)	1029, 1266, 1277
SapI	GCTCTTC	1 cut(s)	1728
SatI	GCNGC	4 cut(s)	432, 478, 663, 1984
Sau3AI	GATC	8 cut(s)	280, 610, 688, 944, 1365, 1812, 1885, 2026
Sau96I	GGNCC	6 cut(s)	423, 424, 499, 646, 763, 961
ScaI	AGTACT	1 cut(s)	1307
SchI	GAGTC	5 cut(s)	50, 79, 331, 1100, 1612
ScrFI	CCNGG	4 cut(s)	1422, 1613, 1874, 2097
SduI	GDGCHC	1 cut(s)	427
SexAI	ACCWGGT	1 cut(s)	1872
SfaNI	GCATC	6 cut(s)	804, 908, 1321, 1338, 1407, 2116
SfcI	CTRYAG	1 cut(s)	429
Sfr274I	CTCGAG	1 cut(s)	202
SinI	GGWCC	3 cut(s)	499, 646, 961
SlaI	CTCGAG	1 cut(s)	202
SmiMI	CAYNNNNRTG	3 cut(s)	1029, 1266, 1277
SmlI	CTYRAG	2 cut(s)	202, 1192
SmoI	CTYRAG	2 cut(s)	202, 1192
SsiI	CCGC	3 cut(s)	793, 1174, 1713
SspMI	CTAG	3 cut(s)	995, 2030, 2117
StyD4I	CCNGG	4 cut(s)	1420, 1611, 1872, 2095
StyI	CCWWGG	4 cut(s)	57, 741, 994, 1267
TaaI	ACNGT	8 cut(s)	214, 416, 643, 725, 862, 1028, 1393, 1705
TaiI	ACGT	1 cut(s)	1047
TaqI	TCGA	6 cut(s)	112, 203, 295, 1231, 1535, 1815
TatI	WGTACW	4 cut(s)	681, 1305, 1665, 2103
TfiI	GAWTC	2 cut(s)	257, 2161
TscAI	CASTG	6 cut(s)	223, 646, 1054, 1066, 1318, 2190
TseFI	GTSAC	4 cut(s)	916, 1045, 1625, 2087
TseI	GCWGC	4 cut(s)	431, 477, 662, 1983
Tsp45I	GTSAC	4 cut(s)	916, 1045, 1625, 2087
TspGWI	ACGGA	1 cut(s)	206
TspRI	CASTG	6 cut(s)	223, 646, 1054, 1066, 1318, 2190
Tth111I	GACNNNGTC	1 cut(s)	1428
Van91I	CCANNNNNTGG	1 cut(s)	871
Vha464I	CTTAAG	1 cut(s)	1192
VpaK11BI	GGWCC	3 cut(s)	499, 646, 961
XagI	CCTNNNNNAGG	1 cut(s)	135
XapI	RAATTY	8 cut(s)	356, 377, 575, 812, 1374, 1545, 1946, 2135
XceI	RCATGY	1 cut(s)	1678
XhoI	CTCGAG	1 cut(s)	202
XmaJI	CCTAGG	1 cut(s)	994
XmnI	GAANNNNTTC	1 cut(s)	256
XspI	CTAG	3 cut(s)	995, 2030, 2117
ZrmI	AGTACT	1 cut(s)	1307
Zsp2I	ATGCAT	1 cut(s)	901

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.