FvH4_2g37840

Belongs to the glycosyl hydrolase 13 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0006625
Physical Location & Seq
Reverse (-)
27466215 .. 27471195
4981 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g37840.t5

Sequence Viewer

Length: 1248 bp
ATGGGTGACCGTAGCATTGTGCAGGATTATCAGGAACACAATCAACAGACTGATATTGGTGCATCAATACGTAATGGAAGAGAAATTCTTTTACAGGCCTTTAACTGGGAATCTCATAAACATGACTGGTGGAAACATTTAGAAAGTAAAGTTCCAGATATTGCAAAATCTGGATTTACATCAGCATGGTTGCCACCATCTACTCATTCCATTGCCCCAGAAGGTTACCTCCCTCAGAACATATATTCCCTCAATTCTAAATATGGATCTGAGCACCAATTAAAAGGTTTACTTCAAAAAATGAATCAATGTAAAGTTAGACCGATGGCTGACATAGTTATCAATCATCGTGTTGGCACTACCCAAGGGCATGGTGGAATATACAATCGTTATGATGGAATTCCATTGTCATGGGATGAACATGCCGTCACATCTTGCACTGGTGGACTGGGGAATCGAAGTACTGGAGACAATTTCAATGGGGTTCCAAATATTGATCATAGTCAACCTTTTGTTAGAAAGGATATTACTGCATGGCTACTGTGGCTTCGCCATACTGTTGGTTTTCAGGATTTTCGATTTGATTTTGCAAAGGGTTATGCAGCTAAATATGTGAAAGAGTATATTGAGGGAGCAAAACCAATTTTTTCTGTTGGGGAGTATTGGAATTCTTGCAACTACAATTCTGATGGCTTGGATTACAATCAAGATAGCCACAGACAGCAGATAATAAATTGGATTGATGGCACAGGACAGCTATCAACTGCATTTGACTTCACAACCAAGGGAATTCTTCAGGAAGCTGTCAAGGGACAATTATGGCGCCTACGTGATCCCCAAGGGAAGCCACCGGGTGTACTTGGATTGTGGCCTTCAAGAGCTGTCACATTCTTAGATAACCATGACACAGGCTCAACACAGGCTCACTGGCCATTCCCTTCAAATCATATCATGGAGGGTTATGCATATATATTCACTCACCCTGGAATACCTTCCGTCTTCTATGACCACTTCTATGATTGGGGTGATTCCATTCATGATCAGATTGTGAAACTGATTCAAATTAGGAAGCAGAAAGACATTCACAGCCGATCATCTATAAGGATCCTGGAGGCCCAGCCAAACCTCTATTCTGCTATAATTGGGGAGAAAGTGTGTATGAAAATTGGGGATAGTTCCTGGTGCCCAGACGTGAGGGAGTGGACGCTGGCAACCTCTGGCGACAGATATGCTGTTTGGCATAAGTAA

Protein Analysis

416

Amino Acids

47.37

Weight (kDa)

6.7

Isoelectric Point (pI)

42.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alpha-amyl_C2 PF07821 311 - 366 3.6e-21 Alpha-amylase C-terminal beta-sheet domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 822, 1182
AclWI GGATC 4 cut(s) 274, 827, 1099, 1112
AcoI YGGCCR 1 cut(s) 929
AcsI RAATTY 4 cut(s) 84, 399, 667, 789
AcuI CTGAAG 1 cut(s) 779
AcyI GRCGYC 1 cut(s) 823
AdeI CACNNNGTG 1 cut(s) 854
AfaI GTAC 2 cut(s) 463, 858
AfiI CCNNNNNNNGG 1 cut(s) 105
AgsI TTSAA 5 cut(s) 296, 478, 876, 942, 1061
AjiI CACGTC 1 cut(s) 1192
AjnI CCWGG 3 cut(s) 982, 1107, 1178
AjuI GAANNNNNNNTTGG 2 cut(s) 1114, 1146
AloI GAACNNNNNNTCC 2 cut(s) 230, 262
AluBI AGCT 4 cut(s) 605, 757, 803, 881
AluI AGCT 4 cut(s) 605, 757, 803, 881
Alw21I GWGCWC 1 cut(s) 276
Alw26I GTCTC 1 cut(s) 462
AlwI GGATC 4 cut(s) 274, 827, 1099, 1112
AoxI GGCC 4 cut(s) 96, 869, 929, 1113
ApeKI GCWGC 1 cut(s) 602
ApoI RAATTY 4 cut(s) 84, 399, 667, 789
Asp700I GAANNNNTTC 1 cut(s) 991
AspLEI GCGC 1 cut(s) 825
AspS9I GGNCC 1 cut(s) 1114
AsuC2I CCSGG 1 cut(s) 852
AsuHPI GGTGA 3 cut(s) 17, 971, 1037
BaeGI GKGCMC 1 cut(s) 1187
BalI TGGCCA 1 cut(s) 931
BamHI GGATCC 1 cut(s) 1104
BanI GGYRCC 2 cut(s) 822, 1182
BbsI GAAGAC 1 cut(s) 991
Bbv12I GWGCWC 1 cut(s) 276
BbvI GCAGC 1 cut(s) 614
BccI CCATC 5 cut(s) 205, 319, 389, 683, 737
BceAI ACGGC 1 cut(s) 410
BcgI CGANNNNNNTGC 2 cut(s) 1211, 1245
BciT130I CCWGG 3 cut(s) 984, 1109, 1180
BclI TGATCA 2 cut(s) 496, 1039
BcnI CCSGG 1 cut(s) 852
BcoDI GTCTC 1 cut(s) 462
BfoI RGCGCY 1 cut(s) 826
BisI GCNGC 1 cut(s) 603
BlsI GCNGC 1 cut(s) 604
BmcAI AGTACT 1 cut(s) 463
Bme1390I CCNGG 4 cut(s) 852, 984, 1109, 1180
BmgBI CACGTC 1 cut(s) 1192
BmgT120I GGNCC 1 cut(s) 1114
BmiI GGNNCC 4 cut(s) 486, 824, 1106, 1184
BmrFI CCNGG 4 cut(s) 852, 984, 1109, 1180
BmrI ACTGGG 2 cut(s) 115, 458
BmsI GCATC 1 cut(s) 71
BmuI ACTGGG 2 cut(s) 115, 458
BpiI GAAGAC 1 cut(s) 991
BpmI CTGGAG 2 cut(s) 486, 1130
BpuMI CCSGG 1 cut(s) 852
BsaAI YACGTR 2 cut(s) 71, 830
BsaBI GATNNNNATC 2 cut(s) 178, 702
BsaHI GRCGYC 1 cut(s) 823
BsaJI CCNNGG 4 cut(s) 364, 783, 838, 982
BsaXI ACNNNNNCTCC 2 cut(s) 1139, 1169
Bsc4I CCNNNNNNNGG 1 cut(s) 105
Bse1I ACTGG 6 cut(s) 110, 131, 445, 453, 469, 932
Bse3DI GCAATG 1 cut(s) 210
Bse8I GATNNNNATC 2 cut(s) 178, 702
BseBI CCWGG 3 cut(s) 984, 1109, 1180
BseDI CCNNGG 4 cut(s) 364, 783, 838, 982
BseGI GGATG 1 cut(s) 421
BseJI GATNNNNATC 2 cut(s) 178, 702
BseLI CCNNNNNNNGG 1 cut(s) 105
BseMI GCAATG 1 cut(s) 210
BseMII CTCAG 2 cut(s) 248, 261
BseNI ACTGG 6 cut(s) 110, 131, 445, 453, 469, 932
BseSI GKGCMC 1 cut(s) 1187
BseXI GCAGC 1 cut(s) 614
BseYI CCCAGC 1 cut(s) 1116
BsgI GTGCAG 1 cut(s) 41
BshFI GGCC 4 cut(s) 98, 871, 931, 1115
BshNI GGYRCC 2 cut(s) 822, 1182
BsiHKAI GWGCWC 1 cut(s) 276
BsiSI CCGG 1 cut(s) 851
BslFI GGGAC 1 cut(s) 825
BslI CCNNNNNNNGG 1 cut(s) 105
BsmAI GTCTC 1 cut(s) 462
BsmFI GGGAC 1 cut(s) 825
BsnI GGCC 4 cut(s) 98, 871, 931, 1115
Bsp1286I GDGCHC 2 cut(s) 276, 1187
Bsp143I GATC 6 cut(s) 266, 496, 832, 1039, 1091, 1104
BspANI GGCC 4 cut(s) 98, 871, 931, 1115
BspCNI CTCAG 2 cut(s) 247, 262
BspHI TCATGA 1 cut(s) 1036
BspLI GGNNCC 4 cut(s) 486, 824, 1106, 1184
BspPI GGATC 4 cut(s) 274, 827, 1099, 1112
BspT107I GGYRCC 2 cut(s) 822, 1182
BsrDI GCAATG 1 cut(s) 210
BsrI ACTGG 6 cut(s) 110, 131, 445, 453, 469, 932
BssECI CCNNGG 4 cut(s) 364, 783, 838, 982
BssMI GATC 6 cut(s) 266, 496, 832, 1039, 1091, 1104
BssNI GRCGYC 1 cut(s) 823
BssT1I CCWWGG 3 cut(s) 364, 783, 838
Bst2UI CCWGG 3 cut(s) 984, 1109, 1180
Bst4CI ACNGT 3 cut(s) 11, 543, 559
Bst6I CTCTTC 1 cut(s) 73
BstACI GRCGYC 1 cut(s) 823
BstBAI YACGTR 2 cut(s) 71, 830
BstC8I GCNNGC 1 cut(s) 1209
BstDEI CTNAG 3 cut(s) 234, 270, 892
BstEII GGTNACC 2 cut(s) 5, 224
BstF5I GGATG 1 cut(s) 421
BstH2I RGCGCY 1 cut(s) 826
BstHHI GCGC 1 cut(s) 825
BstKTI GATC 6 cut(s) 269, 499, 835, 1042, 1094, 1107
BstMAI GTCTC 1 cut(s) 462
BstMBI GATC 6 cut(s) 266, 496, 832, 1039, 1091, 1104
BstMWI GCNNNNNNNGC 1 cut(s) 544
BstNI CCWGG 3 cut(s) 984, 1109, 1180
BstNSI RCATGY 1 cut(s) 425
BstPI GGTNACC 2 cut(s) 5, 224
BstSCI CCNGG 4 cut(s) 850, 982, 1107, 1178
BstSLI GKGCMC 1 cut(s) 1187
BstSNI TACGTA 1 cut(s) 71
BstV1I GCAGC 1 cut(s) 614
BstV2I GAAGAC 1 cut(s) 991
BstX2I RGATCY 2 cut(s) 266, 1104
BstXI CCANNNNNNTGG 3 cut(s) 371, 411, 560
BstYI RGATCY 2 cut(s) 266, 1104
BsuRI GGCC 4 cut(s) 98, 871, 931, 1115
BtrI CACGTC 1 cut(s) 1192
BtsCI GGATG 1 cut(s) 421
BtsIMutI CAGTG 2 cut(s) 438, 925
Cac8I GCNNGC 1 cut(s) 1209
CciI TCATGA 1 cut(s) 1036
CfoI GCGC 1 cut(s) 825
Cfr13I GGNCC 1 cut(s) 1114
CseI GACGC 1 cut(s) 1213
Csp6I GTAC 2 cut(s) 462, 857
CviAII CATG 9 cut(s) 122, 186, 371, 411, 422, 534, 902, 952, 1037
CviQI GTAC 2 cut(s) 462, 857
DdeI CTNAG 3 cut(s) 234, 270, 892
DinI GGCGCC 1 cut(s) 824
DpnI GATC 6 cut(s) 268, 498, 834, 1041, 1093, 1106
DpnII GATC 6 cut(s) 266, 496, 832, 1039, 1091, 1104
DraIII CACNNNGTG 1 cut(s) 854
EaeI YGGCCR 1 cut(s) 929
Eam1104I CTCTTC 1 cut(s) 73
EarI CTCTTC 1 cut(s) 73
Eco105I TACGTA 1 cut(s) 71
Eco130I CCWWGG 3 cut(s) 364, 783, 838
Eco147I AGGCCT 1 cut(s) 98
Eco57I CTGAAG 1 cut(s) 779
Eco91I GGTNACC 2 cut(s) 5, 224
EcoO65I GGTNACC 2 cut(s) 5, 224
EcoRI GAATTC 3 cut(s) 399, 667, 789
EcoRII CCWGG 3 cut(s) 982, 1107, 1178
EcoT14I CCWWGG 3 cut(s) 364, 783, 838
EcoT22I ATGCAT 1 cut(s) 967
EgeI GGCGCC 1 cut(s) 824
EheI GGCGCC 1 cut(s) 824
ErhI CCWWGG 3 cut(s) 364, 783, 838
FaeI CATG 9 cut(s) 125, 189, 374, 414, 425, 537, 905, 955, 1040
FalI AAGNNNNNCTT 2 cut(s) 276, 308
FaqI GGGAC 1 cut(s) 825
FatI CATG 9 cut(s) 121, 185, 370, 410, 421, 533, 901, 951, 1036
FbaI TGATCA 2 cut(s) 496, 1039
Fnu4HI GCNGC 1 cut(s) 603
FokI GGATG 1 cut(s) 428
Fsp4HI GCNGC 1 cut(s) 603
GlaI GCGC 1 cut(s) 824
GluI GCNGC 1 cut(s) 603
GsaI CCCAGC 1 cut(s) 1120
GsuI CTGGAG 2 cut(s) 486, 1130
HaeII RGCGCY 1 cut(s) 826
HaeIII GGCC 4 cut(s) 98, 871, 931, 1115
HapII CCGG 1 cut(s) 851
HgaI GACGC 1 cut(s) 1213
HhaI GCGC 1 cut(s) 825
Hin1I GRCGYC 1 cut(s) 823
Hin1II CATG 9 cut(s) 125, 189, 374, 414, 425, 537, 905, 955, 1040
Hin6I GCGC 1 cut(s) 823
HinP1I GCGC 1 cut(s) 823
HincII GTYRAC 1 cut(s) 506
HindII GTYRAC 1 cut(s) 506
HinfI GANTC 5 cut(s) 110, 304, 454, 1028, 1057
HpaII CCGG 1 cut(s) 851
HphI GGTGA 3 cut(s) 17, 971, 1037
Hpy166II GTNNAC 5 cut(s) 290, 446, 506, 857, 1203
Hpy188I TCNGA 4 cut(s) 237, 271, 688, 1044
Hpy188III TCNNGA 8 cut(s) 32, 155, 171, 569, 707, 797, 876, 1037
Hpy8I GTNNAC 5 cut(s) 290, 446, 506, 857, 1203
HpyAV CCTTC 4 cut(s) 215, 882, 948, 1002
HpyCH4III ACNGT 3 cut(s) 11, 543, 559
HpyCH4IV ACGT 3 cut(s) 70, 829, 1191
HpyF10VI GCNNNNNNNGC 1 cut(s) 544
HpyF3I CTNAG 3 cut(s) 234, 270, 892
HpySE526I ACGT 3 cut(s) 70, 829, 1191
Hsp92I GRCGYC 1 cut(s) 823
Hsp92II CATG 9 cut(s) 125, 189, 374, 414, 425, 537, 905, 955, 1040
HspAI GCGC 1 cut(s) 823
KasI GGCGCC 1 cut(s) 822
Ksp22I TGATCA 2 cut(s) 496, 1039
Kzo9I GATC 6 cut(s) 266, 496, 832, 1039, 1091, 1104
LmnI GCTCC 1 cut(s) 632
Lsp1109I GCAGC 1 cut(s) 614
LweI GCATC 1 cut(s) 71
MaeII ACGT 3 cut(s) 70, 829, 1191
MaeIII GTNAC 4 cut(s) 5, 224, 427, 883
MalI GATC 6 cut(s) 268, 498, 834, 1041, 1093, 1106
MboI GATC 6 cut(s) 266, 496, 832, 1039, 1091, 1104
MboII GAAGA 3 cut(s) 90, 785, 991
MflI RGATCY 2 cut(s) 266, 1104
MhlI GDGCHC 2 cut(s) 276, 1187
MlsI TGGCCA 1 cut(s) 931
MluNI TGGCCA 1 cut(s) 931
Mly113I GGCGCC 1 cut(s) 823
MnlI CCTC 9 cut(s) 239, 243, 260, 622, 949, 1105, 1136, 1188, 1225
Mox20I TGGCCA 1 cut(s) 931
Mph1103I ATGCAT 1 cut(s) 967
MroXI GAANNNNTTC 1 cut(s) 991
MscI TGGCCA 1 cut(s) 931
MseI TTAA 2 cut(s) 102, 281
MslI CAYNNNNRTG 4 cut(s) 120, 184, 409, 1014
Msp20I TGGCCA 1 cut(s) 931
MspI CCGG 1 cut(s) 851
MspR9I CCNGG 4 cut(s) 852, 984, 1109, 1180
MvaI CCWGG 3 cut(s) 984, 1109, 1180
MwoI GCNNNNNNNGC 1 cut(s) 544
NarI GGCGCC 1 cut(s) 823
NciI CCSGG 1 cut(s) 852
NdeII GATC 6 cut(s) 266, 496, 832, 1039, 1091, 1104
NlaIII CATG 9 cut(s) 125, 189, 374, 414, 425, 537, 905, 955, 1040
NlaIV GGNNCC 4 cut(s) 486, 824, 1106, 1184
NmuCI GTSAC 3 cut(s) 5, 427, 883
NsiI ATGCAT 1 cut(s) 967
NspI RCATGY 1 cut(s) 425
PagI TCATGA 1 cut(s) 1036
PceI AGGCCT 1 cut(s) 98
PdmI GAANNNNTTC 1 cut(s) 991
PfeI GAWTC 5 cut(s) 110, 304, 454, 1028, 1057
PfoI TCCNGGA 1 cut(s) 1107
PkrI GCNGC 1 cut(s) 604
PluTI GGCGCC 1 cut(s) 826
Ppu21I YACGTR 2 cut(s) 71, 830
Psp6I CCWGG 3 cut(s) 982, 1107, 1178
PspEI GGTNACC 2 cut(s) 5, 224
PspFI CCCAGC 1 cut(s) 1116
PspGI CCWGG 3 cut(s) 982, 1107, 1178
PspN4I GGNNCC 4 cut(s) 486, 824, 1106, 1184
PspPI GGNCC 1 cut(s) 1114
PsuI RGATCY 2 cut(s) 266, 1104
RsaI GTAC 2 cut(s) 463, 858
RsaNI GTAC 2 cut(s) 462, 857
RseI CAYNNNNRTG 4 cut(s) 120, 184, 409, 1014
SaqAI TTAA 2 cut(s) 102, 281
SatI GCNGC 1 cut(s) 603
Sau3AI GATC 6 cut(s) 266, 496, 832, 1039, 1091, 1104
Sau96I GGNCC 1 cut(s) 1114
ScaI AGTACT 1 cut(s) 463
ScrFI CCNGG 4 cut(s) 852, 984, 1109, 1180
SduI GDGCHC 2 cut(s) 276, 1187
SfaNI GCATC 1 cut(s) 71
SfoI GGCGCC 1 cut(s) 824
SmiMI CAYNNNNRTG 4 cut(s) 120, 184, 409, 1014
SnaBI TACGTA 1 cut(s) 71
SseBI AGGCCT 1 cut(s) 98
SspDI GGCGCC 1 cut(s) 822
SspI AATATT 1 cut(s) 493
StuI AGGCCT 1 cut(s) 98
StyD4I CCNGG 4 cut(s) 850, 982, 1107, 1178
StyI CCWWGG 3 cut(s) 364, 783, 838
TaaI ACNGT 3 cut(s) 11, 543, 559
TaiI ACGT 3 cut(s) 73, 832, 1194
TaqI TCGA 2 cut(s) 457, 577
TaqII GACCGA 1 cut(s) 337
TatI WGTACW 2 cut(s) 461, 856
TfiI GAWTC 5 cut(s) 110, 304, 454, 1028, 1057
Tru1I TTAA 2 cut(s) 102, 281
Tru9I TTAA 2 cut(s) 102, 281
TscAI CASTG 2 cut(s) 445, 932
TseFI GTSAC 3 cut(s) 5, 427, 883
TseI GCWGC 1 cut(s) 602
Tsp45I GTSAC 3 cut(s) 5, 427, 883
TspDTI ATGAA 4 cut(s) 317, 432, 1025, 1175
TspGWI ACGGA 1 cut(s) 985
TspRI CASTG 2 cut(s) 445, 932
XapI RAATTY 4 cut(s) 84, 399, 667, 789
XceI RCATGY 1 cut(s) 425
XcmI CCANNNNNNNNNTGG 1 cut(s) 371
XmnI GAANNNNTTC 1 cut(s) 991
ZrmI AGTACT 1 cut(s) 463
Zsp2I ATGCAT 1 cut(s) 967
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.