Rh6BG463400

Belongs to the glycosyl hydrolase 13 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0006625
Physical Location & Seq
Reverse (-)
67324859 .. 67329578
4720 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG463400.1

Sequence Viewer

Length: 1008 bp
ATGCTTTTGGATACATATGCAGGTGCTTCAATACGTAATGGAAGAGAAATTCTTTTACAGGCCTTTAACTGGGAATCTCATAAACATGACTGGTGGAAAAATTTAGAAAGTAAAGTTCCAGATATTGCAAAATCTGGATTTACATCAGCATGGTTGCCACCATCAACTCATTCCTTTGCCCCGGAAGGTTACCTCCCTCAGAACATATATTCCCTCAATTCTAAATATGGATCTGAGCACCAATTAAAAGGTTTACTTCAAAAAATGAAGCAATGTAAAATTAGACCAATGGCTGACATAGTTATCAATCATCGTGTTGGGACTACCCAAGGGCATGGTGGAATGTACAATCGTTATGATGGAATTCCTTTGTCATGGGATGAACATGCCGTCACATCTTGTACTGGTGGACTGGGGAATCGAAGCACTGGGGACAATTTCAATGGGGTTCCAAATATTGATCATAGTCAACCTTTTGTTAGAAAGGATATTACTACATGGCTACAGTGGCTTCGCAATACTGTTGGTTTTCAGGATTTTCGATTTGATTTCGCAAGGGGTTATGCAGCTAAATATGTGAAAGAGTATATTGAAGGAGCAAAACCAATTTTTTCCGTTGGGGAGTATTGGGATTCTTGCAACTACAATGGTCATGGTTTGGACCACAACCAAGATAGCCACAGACAGCAGATAATAAGTTGGATTGATGGCACCGGACAGCTATCAACTGCATTTGACTTCACAACCAAGGGAATTCTTCAGGAAGCTGTCAATGGACAATTATGGCGATTACGTGATCCCCAAGGGAAGCCACCAGGTTTAATGGGATGGTGGCCTTCAAGATCTGTCACATTCCTAGATAACCATGATACAGGCTCAACACAGGCTCACTGGCCATTCCCTTCAAATCATATTATGGAGGTAAGAAAATGTTGTTCTGGTTGTTGGCTTGTTGTGGTGATATTATTGTGTTCTTGCCCTGGCTGCTGTTATAATCCACTGGTCTAG

Protein Analysis

335

Amino Acids

37.93

Weight (kDa)

7.62

Isoelectric Point (pI)

42.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alpha-amylase PF00128 35 - 105 6.5e-09 Alpha amylase, catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 993
AarI CACCTGC 1 cut(s) 11
Acc36I ACCTGC 1 cut(s) 11
AccB1I GGYRCC 1 cut(s) 710
AclWI GGATC 2 cut(s) 238, 791
AcoI YGGCCR 1 cut(s) 893
AcsI RAATTY 4 cut(s) 48, 100, 363, 753
AcuI CTGAAG 1 cut(s) 743
AfaI GTAC 2 cut(s) 347, 403
AfiI CCNNNNNNNGG 1 cut(s) 69
AgsI TTSAA 6 cut(s) 30, 260, 442, 593, 840, 906
AjnI CCWGG 2 cut(s) 814, 979
AloI GAACNNNNNNTCC 2 cut(s) 194, 226
AluBI AGCT 3 cut(s) 569, 721, 767
AluI AGCT 3 cut(s) 569, 721, 767
Alw21I GWGCWC 1 cut(s) 240
AlwI GGATC 2 cut(s) 238, 791
AoxI GGCC 3 cut(s) 60, 833, 893
ApeKI GCWGC 2 cut(s) 566, 984
ApoI RAATTY 4 cut(s) 48, 100, 363, 753
AspS9I GGNCC 1 cut(s) 661
AsuC2I CCSGG 1 cut(s) 182
AsuHPI GGTGA 1 cut(s) 970
AvaII GGWCC 1 cut(s) 661
BalI TGGCCA 1 cut(s) 895
BanI GGYRCC 1 cut(s) 710
Bbv12I GWGCWC 1 cut(s) 240
BbvI GCAGC 2 cut(s) 578, 971
BccI CCATC 4 cut(s) 169, 353, 701, 822
BceAI ACGGC 1 cut(s) 374
BciT130I CCWGG 2 cut(s) 816, 981
BciVI GTATCC 1 cut(s) 4
BclI TGATCA 1 cut(s) 460
BcnI CCSGG 1 cut(s) 182
BfaI CTAG 2 cut(s) 857, 1006
BfmI CTRYAG 1 cut(s) 503
BfuAI ACCTGC 1 cut(s) 11
BfuI GTATCC 1 cut(s) 4
BglII AGATCT 1 cut(s) 842
BisI GCNGC 2 cut(s) 567, 985
BlsI GCNGC 2 cut(s) 568, 986
Bme1390I CCNGG 3 cut(s) 182, 816, 981
Bme18I GGWCC 1 cut(s) 661
BmgT120I GGNCC 1 cut(s) 661
BmiI GGNNCC 2 cut(s) 450, 712
BmrFI CCNGG 3 cut(s) 182, 816, 981
BmrI ACTGGG 3 cut(s) 79, 422, 438
BmuI ACTGGG 3 cut(s) 79, 422, 438
BpuMI CCSGG 1 cut(s) 182
BsaAI YACGTR 2 cut(s) 35, 794
BsaBI GATNNNNATC 1 cut(s) 142
BsaJI CCNNGG 5 cut(s) 180, 328, 747, 802, 979
BsaWI WCCGGW 1 cut(s) 713
Bsc4I CCNNNNNNNGG 1 cut(s) 69
Bse1I ACTGG 7 cut(s) 74, 95, 409, 417, 433, 896, 1005
Bse3DI GCAATG 1 cut(s) 278
Bse8I GATNNNNATC 1 cut(s) 142
BseBI CCWGG 2 cut(s) 816, 981
BseDI CCNNGG 5 cut(s) 180, 328, 747, 802, 979
BseGI GGATG 2 cut(s) 385, 833
BseJI GATNNNNATC 1 cut(s) 142
BseLI CCNNNNNNNGG 1 cut(s) 69
BseMI GCAATG 1 cut(s) 278
BseMII CTCAG 2 cut(s) 212, 225
BseNI ACTGG 7 cut(s) 74, 95, 409, 417, 433, 896, 1005
BseXI GCAGC 2 cut(s) 578, 971
BshFI GGCC 3 cut(s) 62, 835, 895
BshNI GGYRCC 1 cut(s) 710
BsiHKAI GWGCWC 1 cut(s) 240
BsiSI CCGG 2 cut(s) 182, 714
BslFI GGGAC 2 cut(s) 334, 446
BslI CCNNNNNNNGG 1 cut(s) 69
BsmFI GGGAC 2 cut(s) 334, 446
BsnI GGCC 3 cut(s) 62, 835, 895
Bsp1286I GDGCHC 1 cut(s) 240
Bsp1407I TGTACA 1 cut(s) 345
Bsp143I GATC 4 cut(s) 230, 460, 796, 842
BspANI GGCC 3 cut(s) 62, 835, 895
BspCNI CTCAG 2 cut(s) 211, 226
BspLI GGNNCC 2 cut(s) 450, 712
BspMI ACCTGC 1 cut(s) 11
BspPI GGATC 2 cut(s) 238, 791
BspT107I GGYRCC 1 cut(s) 710
BsrDI GCAATG 1 cut(s) 278
BsrGI TGTACA 1 cut(s) 345
BsrI ACTGG 7 cut(s) 74, 95, 409, 417, 433, 896, 1005
BssECI CCNNGG 5 cut(s) 180, 328, 747, 802, 979
BssMI GATC 4 cut(s) 230, 460, 796, 842
BssT1I CCWWGG 3 cut(s) 328, 747, 802
Bst2UI CCWGG 2 cut(s) 816, 981
Bst4CI ACNGT 2 cut(s) 507, 523
Bst6I CTCTTC 1 cut(s) 37
BstAUI TGTACA 1 cut(s) 345
BstBAI YACGTR 2 cut(s) 35, 794
BstDEI CTNAG 2 cut(s) 198, 234
BstEII GGTNACC 1 cut(s) 188
BstF5I GGATG 2 cut(s) 385, 833
BstKTI GATC 4 cut(s) 233, 463, 799, 845
BstMBI GATC 4 cut(s) 230, 460, 796, 842
BstMWI GCNNNNNNNGC 2 cut(s) 508, 984
BstNI CCWGG 2 cut(s) 816, 981
BstNSI RCATGY 1 cut(s) 389
BstPI GGTNACC 1 cut(s) 188
BstSCI CCNGG 3 cut(s) 180, 814, 979
BstSFI CTRYAG 1 cut(s) 503
BstSNI TACGTA 1 cut(s) 35
BstV1I GCAGC 2 cut(s) 578, 971
BstX2I RGATCY 2 cut(s) 230, 842
BstXI CCANNNNNNTGG 1 cut(s) 335
BstYI RGATCY 2 cut(s) 230, 842
BsuI GTATCC 1 cut(s) 4
BsuRI GGCC 3 cut(s) 62, 835, 895
BtsCI GGATG 2 cut(s) 385, 833
BtsIMutI CAGTG 4 cut(s) 426, 512, 889, 998
BveI ACCTGC 1 cut(s) 11
Cfr13I GGNCC 1 cut(s) 661
CsiI ACCWGGT 1 cut(s) 814
Csp6I GTAC 2 cut(s) 346, 402
CviAII CATG 8 cut(s) 86, 150, 335, 375, 386, 498, 653, 866
CviQI GTAC 2 cut(s) 346, 402
DdeI CTNAG 2 cut(s) 198, 234
DpnI GATC 4 cut(s) 232, 462, 798, 844
DpnII GATC 4 cut(s) 230, 460, 796, 842
EaeI YGGCCR 1 cut(s) 893
Eam1104I CTCTTC 1 cut(s) 37
EarI CTCTTC 1 cut(s) 37
Eco105I TACGTA 1 cut(s) 35
Eco130I CCWWGG 3 cut(s) 328, 747, 802
Eco147I AGGCCT 1 cut(s) 62
Eco47I GGWCC 1 cut(s) 661
Eco57I CTGAAG 1 cut(s) 743
Eco91I GGTNACC 1 cut(s) 188
EcoO65I GGTNACC 1 cut(s) 188
EcoRI GAATTC 2 cut(s) 363, 753
EcoRII CCWGG 2 cut(s) 814, 979
EcoT14I CCWWGG 3 cut(s) 328, 747, 802
ErhI CCWWGG 3 cut(s) 328, 747, 802
FaeI CATG 8 cut(s) 89, 153, 338, 378, 389, 501, 656, 869
FalI AAGNNNNNCTT 2 cut(s) 240, 272
FaqI GGGAC 2 cut(s) 334, 446
FatI CATG 8 cut(s) 85, 149, 334, 374, 385, 497, 652, 865
FauNDI CATATG 1 cut(s) 16
FbaI TGATCA 1 cut(s) 460
Fnu4HI GCNGC 2 cut(s) 567, 985
FokI GGATG 2 cut(s) 392, 840
Fsp4HI GCNGC 2 cut(s) 567, 985
FspBI CTAG 2 cut(s) 857, 1006
GluI GCNGC 2 cut(s) 567, 985
HaeIII GGCC 3 cut(s) 62, 835, 895
HapII CCGG 2 cut(s) 182, 714
Hin1II CATG 8 cut(s) 89, 153, 338, 378, 389, 501, 656, 869
HincII GTYRAC 1 cut(s) 470
HindII GTYRAC 1 cut(s) 470
HinfI GANTC 3 cut(s) 74, 418, 632
HpaII CCGG 2 cut(s) 182, 714
HphI GGTGA 1 cut(s) 970
Hpy166II GTNNAC 3 cut(s) 254, 410, 470
Hpy188I TCNGA 2 cut(s) 201, 235
Hpy188III TCNNGA 5 cut(s) 119, 135, 533, 761, 840
Hpy8I GTNNAC 3 cut(s) 254, 410, 470
HpyAV CCTTC 4 cut(s) 179, 587, 846, 912
HpyCH4III ACNGT 2 cut(s) 507, 523
HpyCH4IV ACGT 2 cut(s) 34, 793
HpyCH4V TGCA 5 cut(s) 20, 128, 566, 639, 731
HpyF10VI GCNNNNNNNGC 2 cut(s) 508, 984
HpyF3I CTNAG 2 cut(s) 198, 234
HpySE526I ACGT 2 cut(s) 34, 793
Hsp92II CATG 8 cut(s) 89, 153, 338, 378, 389, 501, 656, 869
Ksp22I TGATCA 1 cut(s) 460
Kzo9I GATC 4 cut(s) 230, 460, 796, 842
LmnI GCTCC 1 cut(s) 596
Lsp1109I GCAGC 2 cut(s) 578, 971
MabI ACCWGGT 1 cut(s) 814
MaeI CTAG 2 cut(s) 857, 1006
MaeII ACGT 2 cut(s) 34, 793
MaeIII GTNAC 3 cut(s) 188, 391, 847
MalI GATC 4 cut(s) 232, 462, 798, 844
MboI GATC 4 cut(s) 230, 460, 796, 842
MboII GAAGA 2 cut(s) 54, 749
MflI RGATCY 2 cut(s) 230, 842
MhlI GDGCHC 1 cut(s) 240
MlsI TGGCCA 1 cut(s) 895
MluNI TGGCCA 1 cut(s) 895
MmeI TCCRAC 1 cut(s) 680
MnlI CCTC 4 cut(s) 203, 207, 224, 913
Mox20I TGGCCA 1 cut(s) 895
MscI TGGCCA 1 cut(s) 895
MseI TTAA 3 cut(s) 66, 245, 821
MslI CAYNNNNRTG 2 cut(s) 84, 148
Msp20I TGGCCA 1 cut(s) 895
MspI CCGG 2 cut(s) 182, 714
MspR9I CCNGG 3 cut(s) 182, 816, 981
MvaI CCWGG 2 cut(s) 816, 981
MwoI GCNNNNNNNGC 2 cut(s) 508, 984
NciI CCSGG 1 cut(s) 182
NdeI CATATG 1 cut(s) 16
NdeII GATC 4 cut(s) 230, 460, 796, 842
NlaIII CATG 8 cut(s) 89, 153, 338, 378, 389, 501, 656, 869
NlaIV GGNNCC 2 cut(s) 450, 712
NmuCI GTSAC 2 cut(s) 391, 847
NspI RCATGY 1 cut(s) 389
PaqCI CACCTGC 1 cut(s) 11
PceI AGGCCT 1 cut(s) 62
PfeI GAWTC 3 cut(s) 74, 418, 632
PkrI GCNGC 2 cut(s) 568, 986
Ppu21I YACGTR 2 cut(s) 35, 794
PsiI TTATAA 1 cut(s) 993
Psp6I CCWGG 2 cut(s) 814, 979
PspEI GGTNACC 1 cut(s) 188
PspGI CCWGG 2 cut(s) 814, 979
PspN4I GGNNCC 2 cut(s) 450, 712
PspPI GGNCC 1 cut(s) 661
PsuI RGATCY 2 cut(s) 230, 842
RsaI GTAC 2 cut(s) 347, 403
RsaNI GTAC 2 cut(s) 346, 402
RseI CAYNNNNRTG 2 cut(s) 84, 148
SaqAI TTAA 3 cut(s) 66, 245, 821
SatI GCNGC 2 cut(s) 567, 985
Sau3AI GATC 4 cut(s) 230, 460, 796, 842
Sau96I GGNCC 1 cut(s) 661
ScrFI CCNGG 3 cut(s) 182, 816, 981
SduI GDGCHC 1 cut(s) 240
SexAI ACCWGGT 1 cut(s) 814
SfcI CTRYAG 1 cut(s) 503
SinI GGWCC 1 cut(s) 661
SmiMI CAYNNNNRTG 2 cut(s) 84, 148
SnaBI TACGTA 1 cut(s) 35
SseBI AGGCCT 1 cut(s) 62
SspI AATATT 1 cut(s) 457
SspMI CTAG 2 cut(s) 857, 1006
StuI AGGCCT 1 cut(s) 62
StyD4I CCNGG 3 cut(s) 180, 814, 979
StyI CCWWGG 3 cut(s) 328, 747, 802
TaaI ACNGT 2 cut(s) 507, 523
TaiI ACGT 2 cut(s) 37, 796
TaqI TCGA 2 cut(s) 421, 541
TatI WGTACW 2 cut(s) 345, 401
TfiI GAWTC 3 cut(s) 74, 418, 632
Tru1I TTAA 3 cut(s) 66, 245, 821
Tru9I TTAA 3 cut(s) 66, 245, 821
TscAI CASTG 4 cut(s) 433, 512, 896, 1005
TseFI GTSAC 2 cut(s) 391, 847
TseI GCWGC 2 cut(s) 566, 984
Tsp45I GTSAC 2 cut(s) 391, 847
TspDTI ATGAA 2 cut(s) 281, 396
TspGWI ACGGA 1 cut(s) 604
TspRI CASTG 4 cut(s) 433, 512, 896, 1005
VpaK11BI GGWCC 1 cut(s) 661
XapI RAATTY 4 cut(s) 48, 100, 363, 753
XceI RCATGY 1 cut(s) 389
XcmI CCANNNNNNNNNTGG 1 cut(s) 335
XspI CTAG 2 cut(s) 857, 1006
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.