Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0006625

Physical Location & Seq

Genomic Coordinates Reverse (-)

316369 .. 320782

Gene Structure 4414 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1005200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1245 bp

ATGGGTTACCTACGCAGCAATGATTCACGAGAAAACGCGCAGCAGAATGATATTGGTGCAGCAGTGCGCAATGGAAGAGAAATCCTTTTTCAGGCCTTCAATTGGGAGTCTCATAAACATGATTGGTGGAGAAATTTAGAAACAAAAGTTCCGGATATAGGAAGATCTGGTTTTACGTCAGCATGGTTGCCACCAGCAACTCAATCCTTTGCACCAGAAGGTTACCTTCCGCAGGACATATATTCCCTCAATTCTAAATATGGATCCGAGAACTTAATAAAATCTTTACTTCAAAAAATGAAGCAACATAAAGTTAGAGCAATGGCTGACATTGTTATCAATCATCGTGTTGGGACTACCCGAGGGCATGGTGGAATGTACAACCGCTATGATGGAATTTCATTGTCATGGGATGAACGTGCTGTCACATCTTGTACCGGTGGACTGGGTAATCGAAGTACTGGTGACAATTTCCATGGGGTTCCGAATATTGATCATAGCCAACCTTTTGTTCGAAAAGATATTACAGGATGGCTGCAGTGGCTACGTAATAATGTTGGTTTTCAGGATTTCCGATTTGATTTTGCAAGGGGTTATTCAGCAAAATATGTGAAAGAATACATTGAAGGAGCAAAACCAATTTTTTCTGTTGGGGAGTATTGGGACTCTTGCAACTATAACGATCATGGCCTGGATTACAACCAAGATAGCCACAGACAGCGGATAGTAAATTGGATCAATGGCACTGGACAGCTATCAACTGCGTTTGACTTCACAACCAAGGGAATTCTTCAGGAAGCTGTTAAAGGACAATTGTGGCGCCTGCGTGACCCCCAAGGGAAGCCACCAGGTTTAATTGGATGGTGGCCGTCGAGAGCTGTCACATTCCTTGATAACCATGATACAGGCTCAACACAGGCTCATTGGCCCTTCCCTTCAAATCATATTATGGAGGGTTACGCATACATACTCATGCACCCAGGAATACCAACAGTTTTCTACGATCATTTCTATGATTGGGGTGACTCCATTCATGATCAGATTGTGAAACTGATTGACATCAGGAAGCGTCAAGACATTCACAGCCGATCATCTATTACGATCCTGGAGGCCCAGCCGAACCTCTACTCTGCTATAATAGGGGAGAAAATCTGCATGAAAATTGGGGATGGTTCATGGTGCCCGGCCAGTAGGGAGTGGACACTGGCAACCAGCGGCCACAGATATGCTGTTTGGAACAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004553 GO:0004556 GO:0005575 GO:0005576 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0005984 GO:0005985 GO:0005987 GO:0006073 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009311 GO:0009313 GO:0009987 GO:0016052 GO:0016160 GO:0016787 GO:0016798 GO:0043170 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0046352 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko04973 map00500 map01100 map04973

Pfam Domains

Protein Families

Alpha-amyl_C2 Alpha-amylase HIRAN Tyr-DNA_phospho

Protein Analysis

415

Amino Acids

47.42

Weight (kDa)

8.21

Isoelectric Point (pI)

47.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Alpha-amylase	PF00128	53 - 116	1.9e-08	Alpha amylase, catalytic domain
Alpha-amyl_C2	PF07821	357 - 412	1.3e-21	Alpha-amylase C-terminal beta-sheet domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006625)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G76130 AT1G76130
fragaria_vesca	FvH4_2g37840 FvH4_2g37840 FvH4_2g37840 FvH4_2g37840 FvH4_2g37840 FvH4_2g37840 FvH4_2g37840
malus_domestica	MD08G1006000.v1.1 MD15G1005200.v1.1
prunus_persica	Prupe.1G357800_v2.0.a1 Prupe.1G357800_v2.0.a1
pyrus_communis	pycom15g00370
rosa_chinensis	RchiOBHm_Chr6g0303841
rosa_laevigata	RLG00000011039
rosa_multiflora	Rmu_sc0004644.1_g000001 Rmu_sc0007179.1_g000002
rosa_roxburghii	Rroxscaffold_7G00164360
rosa_rugosa	Rorug06G0325300 Rorug06G0325400 Rorug06G0325500
rosa_samantha	Rh6BG463400 Rh6CG451900 Rh6CG452000
rosa_wichuraiana	Rw6G038060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	68
AccB1I	GGYRCC	2 cut(s)	819, 1179
AccII	CGCG	1 cut(s)	38
AccIII	TCCGGA	1 cut(s)	151
AciI	CCGC	4 cut(s)	230, 385, 721, 1215
AclWI	GGATC	4 cut(s)	258, 271, 743, 1096
AcoI	YGGCCR	3 cut(s)	866, 1185, 1216
AcsI	RAATTY	3 cut(s)	133, 396, 786
AcuI	CTGAAG	1 cut(s)	776
AcyI	GRCGYC	1 cut(s)	820
AfaI	GTAC	3 cut(s)	380, 436, 460
AfiI	CCNNNNNNNGG	4 cut(s)	91, 102, 158, 232
AgeI	ACCGGT	1 cut(s)	437
AgsI	TTSAA	4 cut(s)	100, 293, 626, 939
AjnI	CCWGG	4 cut(s)	690, 847, 979, 1104
AjuI	GAANNNNNNNTTGG	2 cut(s)	495, 527
AluBI	AGCT	3 cut(s)	754, 800, 878
AluI	AGCT	3 cut(s)	754, 800, 878
Alw26I	GTCTC	1 cut(s)	114
AlwI	GGATC	4 cut(s)	258, 271, 743, 1096
Ama87I	CYCGRG	1 cut(s)	360
Aor13HI	TCCGGA	1 cut(s)	151
AoxI	GGCC	7 cut(s)	93, 688, 866, 926, 1110, 1185, 1216
ApeKI	GCWGC	4 cut(s)	15, 40, 59, 535
ApoI	RAATTY	3 cut(s)	133, 396, 786
AsiGI	ACCGGT	1 cut(s)	437
AspLEI	GCGC	3 cut(s)	40, 69, 822
AspS9I	GGNCC	2 cut(s)	927, 1111
AsuC2I	CCSGG	1 cut(s)	1184
AsuHPI	GGTGA	2 cut(s)	476, 1034
AsuII	TTCGAA	1 cut(s)	514
AvaI	CYCGRG	1 cut(s)	360
BaeGI	GKGCMC	1 cut(s)	1184
BamHI	GGATCC	1 cut(s)	263
BanI	GGYRCC	2 cut(s)	819, 1179
BauI	CACGAG	1 cut(s)	27
BbvI	GCAGC	4 cut(s)	27, 52, 71, 522
BccI	CCATC	4 cut(s)	386, 525, 855, 1163
BceAI	ACGGC	1 cut(s)	853
BciT130I	CCWGG	4 cut(s)	692, 849, 981, 1106
BclI	TGATCA	2 cut(s)	493, 1036
BcnI	CCSGG	1 cut(s)	1184
BcoDI	GTCTC	1 cut(s)	114
BfmI	CTRYAG	1 cut(s)	536
BfoI	RGCGCY	1 cut(s)	823
BglII	AGATCT	1 cut(s)	164
BisI	GCNGC	5 cut(s)	16, 41, 60, 536, 1216
BlsI	GCNGC	5 cut(s)	17, 42, 61, 537, 1217
BmcAI	AGTACT	1 cut(s)	460
Bme1390I	CCNGG	5 cut(s)	692, 849, 981, 1106, 1184
BmeT110I	CYCGRG	1 cut(s)	360
BmgT120I	GGNCC	2 cut(s)	927, 1111
BmiI	GGNNCC	4 cut(s)	265, 483, 821, 1181
BmrFI	CCNGG	5 cut(s)	692, 849, 981, 1106, 1184
BmrI	ACTGGG	1 cut(s)	455
BmuI	ACTGGG	1 cut(s)	455
BpmI	CTGGAG	1 cut(s)	1127
Bpu14I	TTCGAA	1 cut(s)	514
BpuMI	CCSGG	1 cut(s)	1184
BsaAI	YACGTR	1 cut(s)	548
BsaBI	GATNNNNATC	1 cut(s)	1058
BsaHI	GRCGYC	1 cut(s)	820
BsaJI	CCNNGG	5 cut(s)	361, 475, 780, 835, 979
BsaWI	WCCGGW	2 cut(s)	151, 437
Bsc4I	CCNNNNNNNGG	4 cut(s)	91, 102, 158, 232
Bse118I	RCCGGY	1 cut(s)	437
Bse1I	ACTGG	5 cut(s)	450, 466, 751, 1188, 1209
Bse3DI	GCAATG	3 cut(s)	25, 76, 327
Bse8I	GATNNNNATC	1 cut(s)	1058
BseAI	TCCGGA	1 cut(s)	151
BseBI	CCWGG	4 cut(s)	692, 849, 981, 1106
BseDI	CCNNGG	5 cut(s)	361, 475, 780, 835, 979
BseGI	GGATG	4 cut(s)	418, 536, 866, 1174
BseJI	GATNNNNATC	1 cut(s)	1058
BseLI	CCNNNNNNNGG	4 cut(s)	91, 102, 158, 232
BseMI	GCAATG	3 cut(s)	25, 76, 327
BseNI	ACTGG	5 cut(s)	450, 466, 751, 1188, 1209
BseSI	GKGCMC	1 cut(s)	1184
BseXI	GCAGC	4 cut(s)	27, 52, 71, 522
BseYI	CCCAGC	1 cut(s)	1113
BsgI	GTGCAG	1 cut(s)	78
Bsh1236I	CGCG	1 cut(s)	38
BshFI	GGCC	7 cut(s)	95, 690, 868, 928, 1112, 1187, 1218
BshNI	GGYRCC	2 cut(s)	819, 1179
BshTI	ACCGGT	1 cut(s)	437
BsiHKCI	CYCGRG	1 cut(s)	360
BsiSI	CCGG	3 cut(s)	152, 438, 1184
BslFI	GGGAC	2 cut(s)	367, 677
BslI	CCNNNNNNNGG	4 cut(s)	91, 102, 158, 232
BsmAI	GTCTC	1 cut(s)	114
BsmFI	GGGAC	2 cut(s)	367, 677
BsnI	GGCC	7 cut(s)	95, 690, 868, 928, 1112, 1187, 1218
BsoBI	CYCGRG	1 cut(s)	360
Bsp119I	TTCGAA	1 cut(s)	514
Bsp1286I	GDGCHC	1 cut(s)	1184
Bsp13I	TCCGGA	1 cut(s)	151
Bsp1407I	TGTACA	1 cut(s)	378
Bsp143I	GATC	9 cut(s)	164, 263, 493, 682, 735, 1003, 1036, 1088, 1101
Bsp19I	CCATGG	1 cut(s)	475
BspACI	CCGC	4 cut(s)	230, 385, 721, 1215
BspANI	GGCC	7 cut(s)	95, 690, 868, 928, 1112, 1187, 1218
BspEI	TCCGGA	1 cut(s)	151
BspFNI	CGCG	1 cut(s)	38
BspHI	TCATGA	1 cut(s)	1033
BspLI	GGNNCC	4 cut(s)	265, 483, 821, 1181
BspMAI	CTGCAG	1 cut(s)	540
BspPI	GGATC	4 cut(s)	258, 271, 743, 1096
BspT104I	TTCGAA	1 cut(s)	514
BspT107I	GGYRCC	2 cut(s)	819, 1179
BsrDI	GCAATG	3 cut(s)	25, 76, 327
BsrFI	RCCGGY	1 cut(s)	437
BsrGI	TGTACA	1 cut(s)	378
BsrI	ACTGG	5 cut(s)	450, 466, 751, 1188, 1209
BssAI	RCCGGY	1 cut(s)	437
BssECI	CCNNGG	5 cut(s)	361, 475, 780, 835, 979
BssMI	GATC	9 cut(s)	164, 263, 493, 682, 735, 1003, 1036, 1088, 1101
BssNI	GRCGYC	1 cut(s)	820
BssSI	CACGAG	1 cut(s)	27
BssT1I	CCWWGG	3 cut(s)	475, 780, 835
Bst2BI	CACGAG	1 cut(s)	27
Bst2UI	CCWGG	4 cut(s)	692, 849, 981, 1106
Bst4CI	ACNGT	1 cut(s)	994
Bst6I	CTCTTC	1 cut(s)	70
BstACI	GRCGYC	1 cut(s)	820
BstAUI	TGTACA	1 cut(s)	378
BstBAI	YACGTR	1 cut(s)	548
BstBI	TTCGAA	1 cut(s)	514
BstC8I	GCNNGC	1 cut(s)	824
BstDSI	CCRYGG	1 cut(s)	475
BstEII	GGTNACC	2 cut(s)	5, 221
BstENI	CCTNNNNNAGG	2 cut(s)	89, 230
BstF5I	GGATG	4 cut(s)	418, 536, 866, 1174
BstFNI	CGCG	1 cut(s)	38
BstH2I	RGCGCY	1 cut(s)	823
BstHHI	GCGC	3 cut(s)	40, 69, 822
BstKTI	GATC	9 cut(s)	167, 266, 496, 685, 738, 1006, 1039, 1091, 1104
BstMAI	GTCTC	1 cut(s)	114
BstMBI	GATC	9 cut(s)	164, 263, 493, 682, 735, 1003, 1036, 1088, 1101
BstMWI	GCNNNNNNNGC	1 cut(s)	541
BstNI	CCWGG	4 cut(s)	692, 849, 981, 1106
BstPI	GGTNACC	2 cut(s)	5, 221
BstSCI	CCNGG	5 cut(s)	690, 847, 979, 1104, 1182
BstSFI	CTRYAG	1 cut(s)	536
BstSLI	GKGCMC	1 cut(s)	1184
BstSNI	TACGTA	1 cut(s)	548
BstUI	CGCG	1 cut(s)	38
BstV1I	GCAGC	4 cut(s)	27, 52, 71, 522
BstX2I	RGATCY	2 cut(s)	164, 263
BstYI	RGATCY	2 cut(s)	164, 263
BsuRI	GGCC	7 cut(s)	95, 690, 868, 928, 1112, 1187, 1218
BtgI	CCRYGG	1 cut(s)	475
BtsCI	GGATG	4 cut(s)	418, 536, 866, 1174
BtsI	GCAGTG	2 cut(s)	69, 545
BtsIMutI	CAGTG	4 cut(s)	69, 545, 744, 1202
Cac8I	GCNNGC	1 cut(s)	824
CciI	TCATGA	1 cut(s)	1033
CfoI	GCGC	3 cut(s)	40, 69, 822
Cfr10I	RCCGGY	1 cut(s)	437
Cfr13I	GGNCC	2 cut(s)	927, 1111
CseI	GACGC	1 cut(s)	1058
CsiI	ACCWGGT	1 cut(s)	847
Csp6I	GTAC	3 cut(s)	379, 435, 459
CspAI	ACCGGT	1 cut(s)	437
CviQI	GTAC	3 cut(s)	379, 435, 459
DinI	GGCGCC	1 cut(s)	821
DpnI	GATC	9 cut(s)	166, 265, 495, 684, 737, 1005, 1038, 1090, 1103
DpnII	GATC	9 cut(s)	164, 263, 493, 682, 735, 1003, 1036, 1088, 1101
EaeI	YGGCCR	3 cut(s)	866, 1185, 1216
Eam1104I	CTCTTC	1 cut(s)	70
EarI	CTCTTC	1 cut(s)	70
Eco105I	TACGTA	1 cut(s)	548
Eco130I	CCWWGG	3 cut(s)	475, 780, 835
Eco147I	AGGCCT	1 cut(s)	95
Eco57I	CTGAAG	1 cut(s)	776
Eco88I	CYCGRG	1 cut(s)	360
Eco91I	GGTNACC	2 cut(s)	5, 221
EcoNI	CCTNNNNNAGG	2 cut(s)	89, 230
EcoO65I	GGTNACC	2 cut(s)	5, 221
EcoRI	GAATTC	1 cut(s)	786
EcoRII	CCWGG	4 cut(s)	690, 847, 979, 1104
EcoT14I	CCWWGG	3 cut(s)	475, 780, 835
EgeI	GGCGCC	1 cut(s)	821
EheI	GGCGCC	1 cut(s)	821
ErhI	CCWWGG	3 cut(s)	475, 780, 835
FalI	AAGNNNNNCTT	2 cut(s)	210, 242
FaqI	GGGAC	2 cut(s)	367, 677
FbaI	TGATCA	2 cut(s)	493, 1036
Fnu4HI	GCNGC	5 cut(s)	16, 41, 60, 536, 1216
FokI	GGATG	4 cut(s)	425, 543, 873, 1181
Fsp4HI	GCNGC	5 cut(s)	16, 41, 60, 536, 1216
FspI	TGCGCA	1 cut(s)	68
GlaI	GCGC	3 cut(s)	39, 68, 821
GluI	GCNGC	5 cut(s)	16, 41, 60, 536, 1216
GsaI	CCCAGC	1 cut(s)	1117
GsuI	CTGGAG	1 cut(s)	1127
HaeII	RGCGCY	1 cut(s)	823
HaeIII	GGCC	7 cut(s)	95, 690, 868, 928, 1112, 1187, 1218
HapII	CCGG	3 cut(s)	152, 438, 1184
HgaI	GACGC	1 cut(s)	1058
HhaI	GCGC	3 cut(s)	40, 69, 822
Hin1I	GRCGYC	1 cut(s)	820
Hin6I	GCGC	3 cut(s)	38, 67, 820
HinP1I	GCGC	3 cut(s)	38, 67, 820
HinfI	GANTC	4 cut(s)	23, 107, 665, 1025
HpaII	CCGG	3 cut(s)	152, 438, 1184
HphI	GGTGA	2 cut(s)	476, 1034
Hpy166II	GTNNAC	2 cut(s)	443, 1200
Hpy188I	TCNGA	4 cut(s)	268, 486, 575, 1041
Hpy188III	TCNNGA	8 cut(s)	27, 152, 566, 794, 873, 1034, 1063, 1073
Hpy8I	GTNNAC	2 cut(s)	443, 1200
Hpy99I	CGWCG	1 cut(s)	874
HpyAV	CCTTC	6 cut(s)	106, 212, 236, 620, 940, 945
HpyCH4III	ACNGT	1 cut(s)	994
HpyCH4IV	ACGT	3 cut(s)	176, 418, 547
HpyCH4V	TGCA	7 cut(s)	59, 212, 538, 587, 672, 976, 1155
HpyF10VI	GCNNNNNNNGC	1 cut(s)	541
HpySE526I	ACGT	3 cut(s)	176, 418, 547
Hsp92I	GRCGYC	1 cut(s)	820
HspAI	GCGC	3 cut(s)	38, 67, 820
KasI	GGCGCC	1 cut(s)	819
Kpn2I	TCCGGA	1 cut(s)	151
Ksp22I	TGATCA	2 cut(s)	493, 1036
Kzo9I	GATC	9 cut(s)	164, 263, 493, 682, 735, 1003, 1036, 1088, 1101
LmnI	GCTCC	1 cut(s)	629
Lsp1109I	GCAGC	4 cut(s)	27, 52, 71, 522
MabI	ACCWGGT	1 cut(s)	847
MaeII	ACGT	3 cut(s)	176, 418, 547
MaeIII	GTNAC	8 cut(s)	5, 221, 424, 464, 827, 880, 956, 1022
MalI	GATC	9 cut(s)	166, 265, 495, 684, 737, 1005, 1038, 1090, 1103
MboI	GATC	9 cut(s)	164, 263, 493, 682, 735, 1003, 1036, 1088, 1101
MboII	GAAGA	3 cut(s)	87, 174, 782
MfeI	CAATTG	2 cut(s)	100, 812
MflI	RGATCY	2 cut(s)	164, 263
MhlI	GDGCHC	1 cut(s)	1184
Mly113I	GGCGCC	1 cut(s)	820
MlyI	GAGTC	3 cut(s)	116, 659, 1019
MnlI	CCTC	5 cut(s)	257, 356, 946, 1102, 1133
MroI	TCCGGA	1 cut(s)	151
MseI	TTAA	3 cut(s)	275, 804, 854
MslI	CAYNNNNRTG	5 cut(s)	117, 406, 971, 1011, 1224
MspA1I	CMGCKG	2 cut(s)	721, 1215
MspI	CCGG	3 cut(s)	152, 438, 1184
MspR9I	CCNGG	5 cut(s)	692, 849, 981, 1106, 1184
MunI	CAATTG	2 cut(s)	100, 812
MvaI	CCWGG	4 cut(s)	692, 849, 981, 1106
MvnI	CGCG	1 cut(s)	38
MwoI	GCNNNNNNNGC	1 cut(s)	541
NarI	GGCGCC	1 cut(s)	820
NciI	CCSGG	1 cut(s)	1184
NcoI	CCATGG	1 cut(s)	475
NdeII	GATC	9 cut(s)	164, 263, 493, 682, 735, 1003, 1036, 1088, 1101
NlaIV	GGNNCC	4 cut(s)	265, 483, 821, 1181
NmuCI	GTSAC	5 cut(s)	424, 464, 827, 880, 1022
NsbI	TGCGCA	1 cut(s)	68
NspV	TTCGAA	1 cut(s)	514
PagI	TCATGA	1 cut(s)	1033
PceI	AGGCCT	1 cut(s)	95
PfeI	GAWTC	1 cut(s)	23
PfoI	TCCNGGA	1 cut(s)	1104
PinAI	ACCGGT	1 cut(s)	437
PkrI	GCNGC	5 cut(s)	17, 42, 61, 537, 1217
PleI	GAGTC	3 cut(s)	115, 659, 1019
PluTI	GGCGCC	1 cut(s)	823
PpsI	GAGTC	3 cut(s)	115, 659, 1019
Ppu21I	YACGTR	1 cut(s)	548
Psp6I	CCWGG	4 cut(s)	690, 847, 979, 1104
PspEI	GGTNACC	2 cut(s)	5, 221
PspFI	CCCAGC	1 cut(s)	1113
PspGI	CCWGG	4 cut(s)	690, 847, 979, 1104
PspN4I	GGNNCC	4 cut(s)	265, 483, 821, 1181
PspPI	GGNCC	2 cut(s)	927, 1111
PstI	CTGCAG	1 cut(s)	540
PsuI	RGATCY	2 cut(s)	164, 263
RsaI	GTAC	3 cut(s)	380, 436, 460
RsaNI	GTAC	3 cut(s)	379, 435, 459
RseI	CAYNNNNRTG	5 cut(s)	117, 406, 971, 1011, 1224
SaqAI	TTAA	3 cut(s)	275, 804, 854
SatI	GCNGC	5 cut(s)	16, 41, 60, 536, 1216
Sau3AI	GATC	9 cut(s)	164, 263, 493, 682, 735, 1003, 1036, 1088, 1101
Sau96I	GGNCC	2 cut(s)	927, 1111
ScaI	AGTACT	1 cut(s)	460
SchI	GAGTC	3 cut(s)	116, 659, 1019
ScrFI	CCNGG	5 cut(s)	692, 849, 981, 1106, 1184
SduI	GDGCHC	1 cut(s)	1184
SexAI	ACCWGGT	1 cut(s)	847
SfcI	CTRYAG	1 cut(s)	536
SfoI	GGCGCC	1 cut(s)	821
SfuI	TTCGAA	1 cut(s)	514
SmiMI	CAYNNNNRTG	5 cut(s)	117, 406, 971, 1011, 1224
SnaBI	TACGTA	1 cut(s)	548
SseBI	AGGCCT	1 cut(s)	95
SsiI	CCGC	4 cut(s)	230, 385, 721, 1215
SspDI	GGCGCC	1 cut(s)	819
SspI	AATATT	1 cut(s)	490
StuI	AGGCCT	1 cut(s)	95
StyD4I	CCNGG	5 cut(s)	690, 847, 979, 1104, 1182
StyI	CCWWGG	3 cut(s)	475, 780, 835
TaaI	ACNGT	1 cut(s)	994
TaiI	ACGT	3 cut(s)	179, 421, 550
TaqI	TCGA	3 cut(s)	454, 514, 872
TatI	WGTACW	2 cut(s)	378, 458
TauI	GCSGC	1 cut(s)	1218
TfiI	GAWTC	1 cut(s)	23
Tru1I	TTAA	3 cut(s)	275, 804, 854
Tru9I	TTAA	3 cut(s)	275, 804, 854
TscAI	CASTG	4 cut(s)	69, 545, 751, 1209
TseFI	GTSAC	5 cut(s)	424, 464, 827, 880, 1022
TseI	GCWGC	4 cut(s)	15, 40, 59, 535
Tsp45I	GTSAC	5 cut(s)	424, 464, 827, 880, 1022
TspDTI	ATGAA	6 cut(s)	314, 390, 429, 1022, 1164, 1172
TspRI	CASTG	4 cut(s)	69, 545, 751, 1209
XagI	CCTNNNNNAGG	2 cut(s)	89, 230
XapI	RAATTY	3 cut(s)	133, 396, 786
ZrmI	AGTACT	1 cut(s)	460

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.